Spectroscopic and Hydrodynamic Characterisation of DNA-Linked Gold Nanoparticle Dimers in Solution using Two-Photon Photoluminescence.
- Johanna Midelet,
- Afaf H El-Sagheer,
- Tom Brown,
- Antonios G Kanaras,
- Anne Débarre,
- Martinus H V Werts
Chemphyschem 2018 Feb 21. Epub 2018 Feb 21.
Univ Rennes, CNRS, SATIE-UMR8029, 35000, Rennes, France.
Two-photon photoluminescence (TPPL) emission spectra of DNA-gold nanoparticle (AuNP) monoconjugates and the corresponding DNA-linked AuNP dimers are obtained by photon time-of-flight spectroscopy. This technique is combined with two-photon photoluminescence fluctuation correlation spectroscopy (TPPL-FCS) to simultaneously monitor the optical and hydrodynamic behaviour of these nano-assemblies in solution, with single-particle sensitivity and microsecond temporal resolution. In this study, the AuNPs have an average core diameter of 12 nm, which renders their dark-field plasmonic light scattering too weak for single-particle imaging. Read More