4,629,748 results match your criteria Chemistry Weinheim an der Bergstrasse Germany[Journal]


Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites.

Planta Med 2020 Jul 9. Epub 2020 Jul 9.

Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.

Fungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (, and ) that infest tropical fruits and 57 extracts obtained by their cocultivation. Read More

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http://dx.doi.org/10.1055/a-1200-2046DOI Listing

The impact of (E/Z)-isomerization and aggregation on the color of rhodoxanthin formulations for food and beverages.

Food Chem 2020 Jun 23;332:127370. Epub 2020 Jun 23.

Geisenheim University, Institute of Beverage Research, Analysis and Technology of Plant-based Foods, Von-Lade-Strasse 1, 65366 Geisenheim, Germany. Electronic address:

Rhodoxanthin is a scarcely explored carotenoid with an extraordinarily large chromophore and high potential to function as red pigment. We report broad ranges of color shades accessible with rhodoxanthin across an unprecedented wide range of (E/Z)-ratios by thermal (E/Z)-isomerization, lyotropic aggregation, and two different formulation techniques, covering shares of the (all-E)-configured isomer of 19-91%. At (E/Z)-ratios of 75 and 23%, rhodoxanthin (E/Z)-isomer mixtures in acetone revealed color hues (CIE-h°) ranging from 20-44° to 36-54°. Read More

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http://dx.doi.org/10.1016/j.foodchem.2020.127370DOI Listing

Ultrasonic-assisted synthesis of nano-sized metal-organic framework; a simple method to explore selective and fast Congo Red adsorption.

Ultrason Sonochem 2020 Jul 2;69:105246. Epub 2020 Jul 2.

Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14117-13116, Tehran, Islamic Republic of Iran. Electronic address:

A novel mixed linker Metal-organic Framework, [Co(NHIsoBDC)(bpfn)].DMF (TMU-69), with amide and amino functionalized spacers (bpfn = N,N'-(naphthalene-1,5-diyl)diisonicotinamide, NHIsoBDCH = 5-Aminoisophthalic acid) was synthesized through both solvothermal and ultrasonic approaches. Applying sonochemical irradiation led to ultrafast formation of Flower-shaped nanoplates of TMU-69 within 15 min with high yield while, solvothermal method takes 3 days to form the framework. Read More

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http://dx.doi.org/10.1016/j.ultsonch.2020.105246DOI Listing

Palladium(II) complexes of tridentate bis(benzazole) ligands: Structural, substitution kinetics, DNA interactions and cytotoxicity studies.

J Inorg Biochem 2020 Jun 24;210:111156. Epub 2020 Jun 24.

Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address:

Reactions of 2,6-bis(benzimidazol-2-yl)pyridine (L), 2,6-bis(benzoxazol-2-yl)pyridine (L), and 2,6-bis(benzothiazol-2-yl)pyridine (L) with [Pd(NCMe)Cl] in the presence of NaBF afforded the corresponding Pd(II) complexes, [Pd(L)Cl]BF, PdL; [Pd(L)Cl]BF, PdL; [Pd(L)Cl]BF, PdL; respectively, while reaction of bis[(1H-benzimidazol-2-yl)methyl]amine (L) with [Pd(NCMe)Cl] afforded complex [Pd(L)Cl]Cl, PdL. Characterisation of the complexes was accomplished using NMR, IR, MS, elemental analyses and single crystal X-ray crystallography. Ligand substitution kinetics of these complexes by biological nucleophiles thiourea (Tu), L-methionine (L-Met) and guanosine 5'-diphosphate disodium salt (5-GMP) were examined under pseudo-first order conditions. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2020.111156DOI Listing

Synthesis and evaluation of Na/K-ATP-ase inhibiting and cytotoxic in vitro activities of oleandrigenin and its selected 17β-(butenolidyl)- and 17β-(3-furyl)- analogues.

Eur J Med Chem 2020 Jun 22;202:112520. Epub 2020 Jun 22.

Institute of Organic Chemistry, Polish Academy of Sciences, Ul. Marcina Kasprzaka 44/52, 01-224, Warsaw, Poland. Electronic address:

Natural cardiac-active principles built upon the 14,16β-dihydroxy-5β,14β-androstane core and bearing a heterocyclic substituent at 17β, in particular, a cardenolide - oleandrin and a bufadienolide - bufotalin, are receiving a great deal of attention as potential anticancer drugs. The densely substituted and sterically shielded ring D is the particular structural feature of these compounds. The first synthesis of oleandrigenin from easily available steroid starting material is reported here. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112520DOI Listing

Design, synthesis, and biological evaluation of aryl piperazines with potential as antidiabetic agents via the stimulation of glucose uptake and inhibition of NADH:ubiquinone oxidoreductase.

Eur J Med Chem 2020 Jun 27;202:112416. Epub 2020 Jun 27.

Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland; Maynooth University Human Health Research Institute, Maynooth University, Maynooth, Co. Kildare, Ireland. Electronic address:

The management of blood glucose levels and the avoidance of diabetic hyperglycemia are common objectives of many therapies in the treatment of diabetes. An aryl piperazine compound 3a (RTC1) has been described as a promoter of glucose uptake, in part through a cellular mechanism that involves inhibition of NADH:ubiquinone oxidoreductase. We report herein the synthesis of 41 derivatives of 3a (RTC1) and a systematic structure-activity-relationship study where a number of compounds were shown to effectively stimulate glucose uptake in vitro and inhibit NADH:ubiquinone oxidoreductase. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112416DOI Listing

Validation of a UPDRS-/MDS-UPDRS-based definition of functional dependency for Parkinson's disease.

Parkinsonism Relat Disord 2020 May 30;76:49-53. Epub 2020 May 30.

Institute of Applied Health Sciences, University of Aberdeen, Aberdeen, UK. Electronic address:

Introduction: Functional dependency in basic activities of daily living (ADLs) is a key outcome in Parkinson's disease (PD). We aimed to define dependency in PD, using the original and MDS versions of the Unified Parkinson's Disease Rating Scale (UPDRS).

Methods: We developed two algorithms to define dependency from items of UPDRS Part 2 and MDS-UPDRS Part 2 relating to basic ADLs (feeding, dressing, hygiene and walking, and getting out of a chair). Read More

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http://dx.doi.org/10.1016/j.parkreldis.2020.05.034DOI Listing

The use of green spectroscopy with multivariate calibration models for simultaneous analysis of ternary drug mixture in combined oral antidiabetic pill.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Jun 23;241:118638. Epub 2020 Jun 23.

Pharmaceutical Analysis Department, Faculty of Pharmacy, Menoufia University, Egypt. Electronic address:

In this work, different chemometric calibration models were developed and validated for the purpose of determining of ternary mixture of oral antidiabetic drugs; vildagliptin (VDG), saxagliptin (SAX) and sitagliptin phosphate (STG). The used models were Partial least squares (PLS) and Artificial Neural Networks (ANN). However, on these various models the impact of genetic algorithm (GA) as a form of variable selection was also investigated. Read More

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http://dx.doi.org/10.1016/j.saa.2020.118638DOI Listing

Transcriptomic analysis identifies early cellular and molecular events by which estrogen disrupts testis differentiation and causes feminization in Xenopus laevis.

Aquat Toxicol 2020 Jun 30;226:105557. Epub 2020 Jun 30.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Extensive studies have shown that estrogenic endocrine-disrupting chemicals (EDCs) can disrupt testis differentiation and even cause feminization in vertebrates. However, little is known about the mechanisms by which estrogenic EDCs disrupt testis differentiation. Here, we employed Xenopus laevis, a model amphibian species sensitive to estrogenic EDCs, to explore the molecular and cellular events by which 17β-estradiol (E2) disrupts testis differentiation and causes feminization. Read More

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http://dx.doi.org/10.1016/j.aquatox.2020.105557DOI Listing

Green synthesis and environmental application of iron-based nanomaterials and nanocomposite: A review.

Chemosphere 2020 Jun 28;259:127509. Epub 2020 Jun 28.

Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India. Electronic address:

Green chemistry has been proven to be an efficient route for nanoparticle synthesis. Plant extract based green synthesis of various nanoparticles is extensively studied since the last decade. This paper "Green synthesis and environmental application of Iron-based nanomaterials and nanocomposite: A review" unveils all the possible greener techniques for the synthesis of iron-based nanoparticles and nanocomposites. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2020.127509DOI Listing

Enhancing membrane surface antifouling by implanting amphiphilic polymer brushes using a swelling induced entrapment technique.

Colloids Surf B Biointerfaces 2020 Jun 24;195:111212. Epub 2020 Jun 24.

Research Center for Membrane and Film Technology, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.

In this work, a swelling induced entrapment technique was developed to enhance the hydrophilicity and antifouling performances of polypropylene (PP) microfiltration membranes. By this method, three amphiphilic polymers with different chemical structures (i.e. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2020.111212DOI Listing

TiO microspheres with cross-linked cyclodextrin coating exhibit improved stability and sustained photocatalytic degradation of bisphenol A in secondary effluent.

Water Res 2020 Jun 28;183:116095. Epub 2020 Jun 28.

Nanosystems Engineering Research Center for Nanotechnology Enabled Water Treatment (NEWT), United States; Department of Civil & Environmental Engineering, Houston, TX, 77005, United States. Electronic address:

Photocatalytic water treatment has significant potential to disinfect and degrade recalcitrant organic pollutants while minimizing the need to add chemicals, but current approaches have poor energy efficiency due, in part, to inefficient utilization of photo-generated reactive oxygen species (ROS). Organic coatings such as cyclodextrin (CD) can adsorb target contaminants and bring them close to the photocatalyst surface to enhance ROS utilization efficiency, but the coatings themselves are susceptible to ROS attack. Here, we report an ROS-resistant fluorinated CD polymer (CDP) that can both adsorb contaminants and resist degradation by ROS, yielding a more efficient material for "trap and zap" water treatment. Read More

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http://dx.doi.org/10.1016/j.watres.2020.116095DOI Listing

Voltammetric/UV-Vis study of temozolomide inclusion complexes with cyclodextrin derivatives.

Bioelectrochemistry 2020 Jun 26;136:107587. Epub 2020 Jun 26.

University of Warsaw Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland; Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland. Electronic address:

Temozolomide (TMZ) - a chemotherapeutic agent possessing cytotoxic activity is used in single or combined therapies of human glioma. Difficulties in these applications, connected with low solubility and stability of temozolomide lead us to study the inclusion complexes between TMZ, and three cyclodextrins: β-cyclodextrin (βCD), monodeoxy-6-monoamino-β-cyclodextrin hydrochloride (βCDamine), and β-cyclodextrin containing galactosamine and triazole ring in the side group (βCDgal). The voltammetric and spectroscopy studies showed the improvement of the drug solubility and formation of stable complexes. Read More

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http://dx.doi.org/10.1016/j.bioelechem.2020.107587DOI Listing

BES2019.

Bioelectrochemistry 2020 May 19;136:107554. Epub 2020 May 19.

School of Chemistry, & Ryan Institute, National University of Ireland Galway, Galway, Ireland. Electronic address:

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http://dx.doi.org/10.1016/j.bioelechem.2020.107554DOI Listing

Accelerating high-resolution NMR of half-integer quadrupolar nuclei in solids: SPAM-MQMAS and SPAM-STMAS.

Solid State Nucl Magn Reson 2020 May 13;108:101668. Epub 2020 May 13.

Univ. Lille, CNRS, Centrale Lille, ENSCL, Univ. Artois, UMR 8181 - UCCS - Unit of Catalysis and Chemistry of Solids, F-59000, Lille, France; Bruker Biospin, 34 rue de l'industrie, F-67166, Wissembourg, France; RIKEN RSC NMR Science and Development Division, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama-shi, Kanagawa, 230-0045, Japan. Electronic address:

In solid-state NMR, multiple-quantum MAS (MQMAS) and satellite-transition MAS (STMAS) experiments are well-established techniques to obtain high-resolution spectra of half-integer quadrupolar nuclei. In 2004 and 2005, a soft-pulse-added-mixing (SPAM) concept was introduced by Gan and Amoureux to enhance the S/N ratio of MQMAS and STMAS experiments. Despite their robustness and simplicity, SPAM approaches have not yet been widely applied. Read More

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http://dx.doi.org/10.1016/j.ssnmr.2020.101668DOI Listing

Assessing population exposure to phthalate plasticizers in thirteen Spanish cities through the analysis of wastewater.

J Hazard Mater 2020 Jun 22;401:123272. Epub 2020 Jun 22.

Department of Analytical Chemistry, Institute of Research on Chemical and Biological Analysis (IAQBUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

Phthalates are widely used plasticizers that produce endocrine-disrupting disorders. Quantifying exposure is crucial to perform risk assessments and to develop proper health measures. Herein, a wastewater-based epidemiology approach has been applied to estimate human exposure to six of the mostly used phthalates within the Spanish population. Read More

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http://dx.doi.org/10.1016/j.jhazmat.2020.123272DOI Listing

Arsenic removal from natural groundwater using 'green rust': Solid phase stability and contaminant fate.

J Hazard Mater 2020 Jun 27;401:123327. Epub 2020 Jun 27.

Nano-Science Center, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark.

Arsenic (As) contamination in groundwater remains a pressing global challenge. In this study, we evaluated the potential of green rust (GR), a redox-active iron phase frequently occurring in anoxic environments, to treat As contamination at a former wood preservation site. We performed long-term batch experiments by exposing synthetic GR sulfate (GR) to As-free and As-spiked (6 mg L) natural groundwater at both 25 and 4 °C. Read More

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http://dx.doi.org/10.1016/j.jhazmat.2020.123327DOI Listing

Retraction notice to "Statistical and chemometric view of the variation in the concentration of selected organophosphates in peeled unwashed and unpeeled washed fruits and vegetables" [Food Chem. 300 (2019) 125220].

Authors:
Shima Behkami

Food Chem 2020 Jul 6;330:127445. Epub 2020 Jul 6.

Department of Chemistry, University of Guelph, Guelph, Ontario N1G2W1, Canada; Department of Chemistry, University of Najand, Urmia 5719883826, Iran.

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http://dx.doi.org/10.1016/j.foodchem.2020.127445DOI Listing

Ultra-stable aqueous foams induced by interfacial co-assembly of highly hydrophobic particles and hydrophilic polymer.

J Colloid Interface Sci 2020 Jun 26;579:628-636. Epub 2020 Jun 26.

Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong. Electronic address:

Hypothesis: Interfacial assembly between nanoparticles and complementary ligands can in-situ generate Janus-like particles, rendering high stability of a Pickering-based system. Hence, through the self-assembly and formation of a Janus structure at the air/water interface, which is composed of a hydrophobic particle layer in the inner air phase and then a hydrophilic polymer layer in the aqueous phase, it should be possible to fabricate ultra-stable Pickering foams.

Experiments: Foams containing different highly hydrophobic particles and hydrophilic polymers or microgels were prepared, and their stability was investigated. Read More

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http://dx.doi.org/10.1016/j.jcis.2020.06.098DOI Listing

Ag-Pi/BiVO heterojunction with efficient interface carrier transport for photoelectrochemical water splitting.

J Colloid Interface Sci 2020 Jun 30;579:619-627. Epub 2020 Jun 30.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:

The limitation of pristine BiVO photoanode severely causes the high carrier recombination efficiency and low energy conversion efficiency in the photoelectrochemical (PEC) system. In this work, the Ag-Pi/BiVOn-n heterojunction has been rationally designed and fabricated for efficient PEC water splitting, through successive ionic layer adsorption reaction method. The built-in field of Ag-Pi/BiVO significantly promotes the separation efficiency of photogenerated carriers, benefiting for the participation of abundant holes in water oxidation. Read More

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http://dx.doi.org/10.1016/j.jcis.2020.06.108DOI Listing

Electrochemical hydrogen and oxygen evolution reactions from a cobalt-porphyrin-based covalent organic polymer.

J Colloid Interface Sci 2020 Jun 30;579:598-606. Epub 2020 Jun 30.

School of Energy & Power Engineering, School of Chemistry & Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:

Covalent organic polymers have attracted much attention due to their high specific surface area, superlative porosity, and diversity in electronic structure. Herein, a novel porous cobalt-porphyrin-based covalent organic polymer (CoCOP) is fabricated through the Schiff-base condensation reaction, which is used as a difunctional electrocatalyst for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). The CoCOP possesses a high surface area and strong synergistic effect between the cobalt-porphyrins and the CN groups, resulting in efficient HER and OER performances. Read More

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http://dx.doi.org/10.1016/j.jcis.2020.06.109DOI Listing

Novel biocompatible and antimicrobial supramolecular O-carboxymethyl chitosan biguanidine/zinc physical hydrogels.

Int J Biol Macromol 2020 Jul 6. Epub 2020 Jul 6.

Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt. Electronic address:

Zinc crosslinked supramolecular hydrogels from O-carboxymethyl chitosan biguanidine were successfully prepared. The structures of the prepared hydrogels were verified by HNMR, FTIR and XRD. FE-SEM verified the porous structure of hydrogels especially at high contents of Zn ions. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.029DOI Listing

Improved immobilization of lipase from Thermomyces lanuginosus on a new chitosan-based heterofunctional support: Mixed ion exchange plus hydrophobic interactions.

Int J Biol Macromol 2020 Jul 6. Epub 2020 Jul 6.

Graduate Program in Biotechnology, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil; Institute of Chemistry, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil. Electronic address:

In this study, a new mixed heterofunctional support (Chit-GA-Gly) has been prepared by sequential activation of chitosan hydrogel (Chit) with glutaraldehyde (GA) and further functionalization with glycine (Gly). The immobilization of the lipase from Thermomyces lanuginosus (TLL) on this support was compared with that on GA-activated Chit hydrogel (Chit-GA). The supports have been characterized by FT-IR, zeta potential and TG analyses. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.021DOI Listing

On an effective approach to improve the properties and the drug release of chitosan-based microparticles.

Int J Biol Macromol 2020 Jul 6. Epub 2020 Jul 6.

Department of Chemistry and Industrial Chemistry, University of Genoa, Via Dodecaneso 31, 16146 Genoa, Italy. Electronic address:

The work investigated the possibility to develop an easy scalable treatment capable of modifying only the surface of chitosan-based materials, limiting the degradation of the bulk and the burst release of a drug, without compromising the properties of the polymeric matrix. To this aim, microparticles of CHI were superficially coated with poly-(styrene-co-maleic anhydride) (PSMA), taking advantage of the potential reactivity of chitosan amino groups and maleic functionalities of PSMA. The specific reactions/interactions occurring between the two polymers were studied by IR measurements, while FE-SEM analysis evidenced the modification of the morphology of the particles contacted with PSMA. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.016DOI Listing

Synthesis and anticancer activity of some pyrimidine derivatives with aryl urea moieties as apoptosis-inducing agents.

Bioorg Chem 2020 Jun 18;101:104028. Epub 2020 Jun 18.

Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.

A new series of pyrimidine derivatives containing aryl urea moieties was designed and synthesized. The anticancer activities of all compounds were evaluated in vitro against colon and prostat cancer cell lines by MTT assay. Among these compounds, 4b exhibited the highest cytotoxic activity against SW480 cancer cell line with IC value of 11. Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.104028DOI Listing

Cadmium Induces Iron Deficiency Anemia through the Suppression of Iron Transport in the Duodenum.

Toxicol Lett 2020 Jul 6. Epub 2020 Jul 6.

Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University, Nagoya, 464-8650, Japan. Electronic address:

Cadmium (Cd) is an environmental contaminant that triggers toxic effects in various tissues such as the kidney, liver, and lung. Cd can also cause abnormal iron metabolism, leading to anemia. Iron homeostasis is regulated by intestinal absorption. Read More

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http://dx.doi.org/10.1016/j.toxlet.2020.07.005DOI Listing

Photodynamic Inactivation using a Chlorin-Based Photosensitizer with Blue or Red-Light Irradiation against Single-species Biofilms related to Periodontitis.

Photodiagnosis Photodyn Ther 2020 Jul 6:101916. Epub 2020 Jul 6.

Department of Diagnosis and Surgery, São Paulo State University (Unesp), School of Dentistry, Rua Humaitá, 1680, 14801-903, Araraquara, SP, Brazil. Electronic address:

Chlorin-e6 (Ce6), as a photosensitizer (PS), has demonstrated significant reduction of microorganisms' viability when irradiated by red light. However, the main absorption peak of this PS is located at blue light spectrum, never investigated. This study aimed to evaluate the effect of pure-chlorin-e6-mediated photodynamic inactivation (PDI) using different light sources (450 or 660 nm) against biofilms related to periodontitis. Read More

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http://dx.doi.org/10.1016/j.pdpdt.2020.101916DOI Listing

Heavy metal contamination in recorded and unrecorded spirits. Should we worry?

Regul Toxicol Pharmacol 2020 Jul 6:104723. Epub 2020 Jul 6.

Department of Preventive Medicine, Faculty of Public Health, University of Debrecen, Debrecen, Hungary. Electronic address:

Heavy metals can be released into all alcoholic beverages during production and storage. However, there is at least a theoretical risk that they could be present in higher, and potentially toxic, concentrations in those produced in the household and small-scale stills common in Central and Eastern Europe, which lack quality control and whose products are unrecorded by authorities. Yet, so far, few studies comparing concentrations of heavy metals in recorded and unrecorded spirits have been published. Read More

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http://dx.doi.org/10.1016/j.yrtph.2020.104723DOI Listing

Human Articular Cartilage is Orthotropic where Microstructure, Micromechanics, and Chemistry Vary with Depth and Split-line Orientation.

Osteoarthritis Cartilage 2020 Jul 6. Epub 2020 Jul 6.

Department of Mechanical Engineering, University of Colorado, Boulder, CO; BioFrontiers Institute, University of Colorado, Boulder, CO; Biomedical Engineering Program, University of Colorado, Boulder, CO. Electronic address:

Objective: Quantitative, micrometer length scale assessment of human articular cartilage is essential to enable progress toward new functional tissue engineering approaches, including utilization of emerging 3D bioprinting technologies, and for improved computational modeling of the osteochondral unit. Thus the objective of this study was to characterize the structural organization, material properties, and chemical composition of human skeletally mature articular cartilage with respect to depth and defined morphological features: normal to the articulating surface, parallel to the split-line, and transverse to the split-line.

Method: Three samples from the lateral femoral condyles of 4 healthy adult donors (55-61 years old) were evaluated via histology, second harmonic generation, microindentation, and Raman spectroscopy. Read More

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http://dx.doi.org/10.1016/j.joca.2020.06.007DOI Listing

RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.

Mol Cell 2020 Jun 30. Epub 2020 Jun 30.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 26 Qiuyue Road, Pudong New District, Shanghai 201203, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Shijingshan District, Beijing 100049, China. Electronic address:

Valosin-containing protein (VCP)/p97 is an AAA-ATPase that extracts polyubiquitinated substrates from multimeric macromolecular complexes and biological membranes for proteasomal degradation. During p97-mediated extraction, the substrate is largely deubiquitinated as it is threaded through the p97 central pore. How p97-extracted substrates are targeted to the proteasome with few or no ubiquitins is unknown. Read More

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http://dx.doi.org/10.1016/j.molcel.2020.06.023DOI Listing

Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling.

Mol Cell 2020 Jul 4. Epub 2020 Jul 4.

Department of Biology, Stanford University, Stanford, CA, USA. Electronic address:

Short linear motifs (SLiMs) drive dynamic protein-protein interactions essential for signaling, but sequence degeneracy and low binding affinities make them difficult to identify. We harnessed unbiased systematic approaches for SLiM discovery to elucidate the regulatory network of calcineurin (CN)/PP2B, the Ca-activated phosphatase that recognizes LxVP and PxIxIT motifs. In vitro proteome-wide detection of CN-binding peptides, in vivo SLiM-dependent proximity labeling, and in silico modeling of motif determinants uncovered unanticipated CN interactors, including NOTCH1, which we establish as a CN substrate. Read More

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http://dx.doi.org/10.1016/j.molcel.2020.06.029DOI Listing

A scoutRNA Is Required for Some Type V CRISPR-Cas Systems.

Mol Cell 2020 Jun 30. Epub 2020 Jun 30.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Gladstone Institutes, University of California, San Francisco, San Francisco, CA 94114, USA. Electronic address:

CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection. However, Cas12c (C2c3)- and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observed. We show here that a short-complementarity untranslated RNA (scoutRNA), together with crRNA, is required for Cas12d-catalyzed DNA cutting. Read More

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http://dx.doi.org/10.1016/j.molcel.2020.06.022DOI Listing

Kolaviron Stimulates Glucose Uptake with Concomitant Modulation of Metabolic Activities Implicated in Neurodegeneration in Isolated Rat Brain, without Pertubation of Tissue Ultrastructural Morphology.

Neurosci Res 2020 Jul 6. Epub 2020 Jul 6.

Department of Biochemistry, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa. Electronic address:

Reduced glucose uptake usually occurs in type 2 diabetes due to down-regulation of brain glucose transporters. The potential of kolaviron, a biflavonoid from Garcinia kola to stimulate glucose uptake and suppress glucose-induced oxidative toxicity were investigated in rat brain. Its molecular interactions with the target proteins were investigated in silico. Read More

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http://dx.doi.org/10.1016/j.neures.2020.06.008DOI Listing

Glycosylated-Imidazole Aldoximes as Reactivators of Pesticides Inhibited AChE: Synthesis and In-vitro Reactivation Study.

Environ Toxicol Pharmacol 2020 Jul 6:103454. Epub 2020 Jul 6.

University of Hradec Kralove, Faculty of Science, Department of Chemistry, Rokitanskeho 62, Hradec Kralove, Czech Republic; University Hospital, Biomedical Research Center, Sokolska 581, 50005, Hradec Kralove, Czech Republic. Electronic address:

The present armamentarium of commercially available antidotes provides limited protection against the neurological effects of organophosphate exposure. Hence, there is an urgent need to design and develop molecules that can protect and reactivate inhibited-AChE in the central nervous system. Some natural compounds like glucose and certain amino acids (glutamate, the anion of glutamic acid) can easily cross the blood brain barrier although they are highly polar. Read More

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http://dx.doi.org/10.1016/j.etap.2020.103454DOI Listing

The interactions of a number of steroid-metabolizing cytochromes P450 with abiraterone D4A metabolite: spectral analysis and molecular docking.

Steroids 2020 Jul 6:108693. Epub 2020 Jul 6.

Pirogov Russian National Research Medical University, Ostrovitianov Street, 1, Moscow 117997, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, Moscow 119121, Russia. Electronic address:

The interactions of pharmacologically active 3-keto-Δ4-metabolite of anticancer drug abiraterone (D4A) with steroid-metabolizing cytochromes P450 (CYP51A1, CYP11A1, CYP19A1) was studied by absorption spectroscopy and molecular docking. Both abiraterone and D4A induce type I spectral changes of CYP51A1, one of the enzymes of cholesterol biosynthesis. We have revealed that D4A did not induce spectral changes of CYP11A1, the key enzyme of pregnenolone biosynthesis, unlike abiraterone. Read More

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http://dx.doi.org/10.1016/j.steroids.2020.108693DOI Listing

Structures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies.

Cell 2020 Jun 24. Epub 2020 Jun 24.

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. Electronic address:

Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal immunoglobulin Gs (IgGs) and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their focus on RBD epitopes, recognition of alpha- and beta-coronaviruses, and contributions of avidity to increased binding/neutralization of IgGs over Fabs. Read More

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http://dx.doi.org/10.1016/j.cell.2020.06.025DOI Listing

Single-and repeat-dose toxicity of HM10760A, a long-acting erythropoietin, in rats and monkeys.

Toxicol Appl Pharmacol 2020 Jul 6:115126. Epub 2020 Jul 6.

Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea. Electronic address:

Anemia is a frequent complication of chronic kidney disease (CKD) that causes an increase in morbidity and mortality and accelerates the rate of disease progression. Treatment with recombinant human erythropoietin (rhEPO) is a major breakthrough in the therapy of renal anemia. HM10760A, a long-acting EPO, has been developed as a treatment for anemia in CKD patients. Read More

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http://dx.doi.org/10.1016/j.taap.2020.115126DOI Listing

From Synapse to Supper: A Food Preference Recipe with Olfactory Synaptic Ingredients.

Neuron 2020 Jul;107(1):8-11

Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Rua Monteiro Lobato, s/n, Campinas, Sao Paulo 13083-862, Brazil. Electronic address:

C1ql3 protein and its receptor Bai3 are involved in synaptic organization and function. In this issue of Neuron, Wang et al. (2020) report that both are essential for synaptic function between the anterior olfactory nucleus and the olfactory bulb and for the generation, but not recall, of associative olfactory memories determining food preference in mice. Read More

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http://dx.doi.org/10.1016/j.neuron.2020.06.016DOI Listing

Spatiotemporal PET Imaging Reveals Differences in CAR-T Tumor Retention in Triple-Negative Breast Cancer Models.

Mol Ther 2020 Jun 27. Epub 2020 Jun 27.

Imaging Therapies and Cancer Group, Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences, King's College London, London, UK. Electronic address:

Chimeric antigen receptor T cell therapy (CAR-T) has been rolled out as a new treatment for hematological malignancies. For solid tumor treatment, CAR-T has been disappointing so far. Challenges include the quantification of CAR-T trafficking, expansion and retention in tumors, activity at target sites, toxicities, and long-term CAR-T survival. Read More

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http://dx.doi.org/10.1016/j.ymthe.2020.06.028DOI Listing

Mechanism of Nucleotidyltransfer Reaction and Role of Mg Ion in Sugar Nucleotidyltransferases.

Biophys J 2020 Jun 24. Epub 2020 Jun 24.

Department of Chemistry, Indian Institute of Technology, Kanpur, India. Electronic address:

Sugar nucleotidyl transferases (SNTs) catalyze nucleotidyltransfer reactions to form sugar-nucleotides and pyrophosphate in the presence of two Mg ions (Mg and Mg). We unveil the mechanism and free energetics of nucleotidyl transfer reaction in an SNT called GlmU through hybrid quantum mechanics-molecular mechanics molecular dynamics simulations and free energy calculations. The study identifies the roles of the active site residues and the Mg ions in catalyzing the reaction. Read More

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http://dx.doi.org/10.1016/j.bpj.2020.06.017DOI Listing

The COVID-19 pandemic.

Crit Rev Clin Lab Sci 2020 Jul 9:1-24. Epub 2020 Jul 9.

Department of Experimental Medicine, University of Tor Vergata, Rome, Italy.

In December 2019, an outbreak of pneumonia of unknown origin was reported in Wuhan, Hubei Province, China. Pneumonia cases were epidemiologically linked to the Huanan Seafood Wholesale Market. Inoculation of respiratory samples into human airway epithelial cells, Vero E6 and Huh7 cell lines, led to the isolation of a novel respiratory virus whose genome analysis showed it to be a novel coronavirus related to SARS-CoV, and therefore named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Read More

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http://dx.doi.org/10.1080/10408363.2020.1783198DOI Listing

Mechanistic studies on the drug metabolism and toxicity originating from cytochromes P450.

Drug Metab Rev 2020 Jul 9:1-29. Epub 2020 Jul 9.

Department of Medicinal Chemistry and Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Punjab, India.

Cytochromes P450 are oxidizing enzymes; a few families of cytochromes P450 are implicated in drug metabolism. These enzymatic reactions involve many processes including (i) prodrug to drug conversion, (ii) easy excretion of drug, (iii) generation of reactive metabolites, many of which cause toxicity. In this review, the fundamental biochemical mechanisms associated with the conversion of drugs into the useful or toxic metabolites have been discussed. Read More

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http://dx.doi.org/10.1080/03602532.2020.1765792DOI Listing

Substituent Effect Induces Emission Modulation of Stilbene Photoswitches by Spatial Tuning of the N/B Electronic Constraints.

Org Lett 2020 Jul 9. Epub 2020 Jul 9.

Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology of China, Beijing 102488, People's Republic of China.

We present a family of stilbene derivatives (-) ortho-functionalized with an electron donor and acceptor. Their emission was tuned by donor substitutions, dictating charge transfer dependent on the N···B spatial separation. The steric reduction of substituents on N leads to stronger emission responses upon trans-to-cis photoisomerization. Read More

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http://dx.doi.org/10.1021/acs.orglett.0c01777DOI Listing

Unusual Reactivity of 4-Vinyl Isoxazoles in the Copper-Mediated Synthesis of Pyridines, Employing DMSO as a One-Carbon Surrogate.

Org Lett 2020 Jul 9. Epub 2020 Jul 9.

Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462066, MP, India.

An efficient protocol for the synthesis of nicotinate derivatives and tetrasubstituted pyridines through a copper-mediated cleavage of isoxazoles has been developed. The highlight of the work is the observation of an unusual reactivity of 4-vinyl isoxazoles under the reaction conditions. DMSO serves as a one-carbon surrogate generating an active methylene group during the reaction to form two C-C bonds. Read More

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http://dx.doi.org/10.1021/acs.orglett.0c01935DOI Listing

Highly Selective and Divergent Acyl and Aryl Cross-Couplings of Amides via Ir-Catalyzed C-H Borylation/N-C(O) Activation.

Org Lett 2020 Jul 9. Epub 2020 Jul 9.

Department of Chemistry, Rutgers University, 73 Warren Street, Newark, New Jersey 07102, United States.

Herein, we demonstrate that amides can be readily coupled with nonactivated arenes via sequential Ir-catalyzed C-H borylation/N-C(O) activation. This methodology provides facile access to biaryl ketones and biaryls by the sterically controlled Ir-catalyzed C-H borylation and divergent acyl and decarbonylative amide N-C(O) and C-C activation. The methodology diverts the traditional acylation and arylation regioselectivity, allowing us to directly utilize readily available arenes and amides to produce valuable ketone and biaryl motifs. Read More

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http://dx.doi.org/10.1021/acs.orglett.0c02105DOI Listing

Structural and Optical Properties of Solvated PbI in γ-Butyrolactone: Insight into the Solution Chemistry of Lead Halide Perovskite Precursors.

J Phys Chem Lett 2020 Jul 9. Epub 2020 Jul 9.

We employ a fine-tuned theoretical framework, combining ab-initio molecular dynamics (AIMD), density functional theory (DFT) and time-dependent (TD) DFT methods, to investigate the interactions and optical properties of the iodoplumbates within the low coordinative γ-butyrolactone (GBL) solvent environment, widely employed in the perovskite synthesis. We uncover the extent of GBL coordination to PbI2 investigating its relation to the solvated PbI2 optical properties. The employed approach has been further validated by comparison with the experimental UV-Vis absorption spectra of PbI2 in GBL solvent. Read More

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http://dx.doi.org/10.1021/acs.jpclett.0c01890DOI Listing

Predicting the substrate scope of the flavin-dependent halogenase BrvH.

Chembiochem 2020 Jul 9. Epub 2020 Jul 9.

University of Bielefeld, Department of Organic Chemistry, PO Box 100131, 33501, Bielefeld, GERMANY.

The recently described flavin-dependent halogenase BrvH is able to catalyze both bromination and chlorination of indole, but shows significantly higher bromination activity. BrvH was annotated as a tryptophan halogenase, but does not accept tryptophan as a substrate. Its native substrate remains unknown. Read More

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http://dx.doi.org/10.1002/cbic.202000444DOI Listing

Mechanistic characterization of UDP-glucuronic acid 4-epimerase.

FEBS J 2020 Jul 9. Epub 2020 Jul 9.

Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWI Graz, Petersgasse 12, 8010, Graz, Austria.

UDP-glucuronic acid (UDP-GlcA) is a central precursor in sugar nucleotide biosynthesis and common substrate for C4-epimerases and decarboxylases releasing UDP-galacturonic acid (UDP-GalA) and UDP-pentose products, respectively. Despite the different reactions catalyzed, the enzymes are believed to share mechanistic analogy rooted in their joint membership to the short-chain dehydrogenase/reductase (SDR) protein superfamily: oxidation at the substrate C4 by enzyme-bound NAD initiates the catalytic pathway. Here, we present mechanistic characterization of the C4-epimerization of UDP-GlcA, which in comparison to the corresponding decarboxylation has been largely unexplored. Read More

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http://dx.doi.org/10.1111/febs.15478DOI Listing

Half a century of Scots pine forest ecosystem monitoring reveals long-term effects of atmospheric deposition and climate change.

Glob Chang Biol 2020 Jul 9. Epub 2020 Jul 9.

Bavarian State Institute of Forestry, Hans-Carl-von-Carlowitz-Platz 1, 85354, Freising, Germany.

At two forest sites in Germany (Pfaffenwinkel, Pustert) stocked with mature Scots pine (Pinus sylvestris L.), we investigated changes of topsoil chemistry during the recent forty years by soil inventories conducted on replicated control plots of fertilization experiments, allowing a statistical analysis. Additionally, we monitored the nutritional status of both stands from 1964 until 2019 and quantified stand growth during the monitoring period by repeated stand inventories. Read More

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http://dx.doi.org/10.1111/gcb.15265DOI Listing
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