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Spiral column configuration for protein separation by high-speed countercurrent chromatography.

Yoichiro Ito

Chem Eng Process 2010 Jul;49(7):782-792

Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Room 8N230, 10 Center Drive, Bethesda, MD 20892-1762, USA.

Retention of the stationary phase of aqueous-aqueous polymer phase systems is improved by a spiral column configuration which utilizes the radially acting centrifugal force along the spiral pitch to retain the heavier phase in the outer portion and the lighter phase in the inner portion of the spiral channel. For the separation of proteins which has low mass transfer rates, the system needs further modification of the separation channel to interrupt the laminar flow and enhance mixing of the two phases. Two spiral column assemblies were developed, one using a disk with spiral grooves and the other, the spiral tube support which accommodates the multiple spiral layers made from a single piece of fluorinated plastic tubing. Read More

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http://dx.doi.org/10.1016/j.cep.2009.08.003DOI Listing
July 2010
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