Dev Cell 2021 Apr 15;56(7):1000-1013.e6. Epub 2021 Mar 15.
University of Cambridge, Department of Genetics, Downing Street, Cambridge CB23EH, UK; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E, California Blvd, Pasadena, CA 91125, USA. Electronic address:
Lysosome-related organelles (LROs) are endosomal compartments carrying tissue-specific proteins, which become enlarged in Chediak-Higashi syndrome (CHS) due to mutations in LYST. Here, we show that Drosophila Mauve, a counterpart of LYST, suppresses vesicle fusion events with lipid droplets (LDs) during the formation of yolk granules (YGs), the LROs of the syncytial embryo, and opposes Rab5, which promotes fusion. Mauve localizes on YGs and at spindle poles, and it co-immunoprecipitates with the LDs' component and microtubule-associated protein Minispindles/Ch-TOG. Read More