18,433 results match your criteria Chagas Disease American Trypanosomiasis


Analysis of COVID-19 under-reporting in Brazil.

Rev Bras Ter Intensiva 2020 Jun 24. Epub 2020 Jun 24.

Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz - Rio de Janeiro (RJ), Brasil.

Objective: To estimate the reporting rates of coronavirus disease 2019 (COVID-19) cases for Brazil as a whole and states.

Methods: We estimated the actual number of COVID-19 cases using the reported number of deaths in Brazil and each state, and the expected case-fatality ratio from the World Health Organization. Brazil's expected case-fatality ratio was also adjusted by the population's age pyramid. Read More

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http://dx.doi.org/10.5935/0103-507X.20200030DOI Listing

Spatial distribution of Chagas disease and its correlation with health services.

Rev Esc Enferm USP 2020 26;54:e03565. Epub 2020 Jun 26.

Universidade do Estado do Pará, Escola de Enfermagem Magalhães Barata, Belém, PA, Brazil.

Objective: To analyze the spatial distribution of Acute Chagas Disease (ACD) cases in a riverside municipality and make relationships with the notifying health services.

Method: Longitudinal, ecological, quantitative study with use of geoprocessing techniques, conducted with cases of acute Chagas disease in the municipality of Abaetetuba/Pará.

Results: Inclusion of 204 disease cases. Read More

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http://dx.doi.org/10.1590/S1980-220X2018058603565DOI Listing

BOWEL FREQUENCY AND SYMPTOMS OF CONSTIPATION AND ITS RELATION WITH THE LEVEL OF PHYSICAL ACTIVITY IN PATIENTS WITH CHAGAS DISEASE.

Arq Gastroenterol 2020 Apr-Jun;57(2):161-166

Universidade Estadual de Maringá, Departamento de Ciências Básicas da Saúde, Parasitologia, Maringá, PR, Brasil.

Background: Intestinal constipation (IC) in patients with the digestive form of Chagas disease is one of the main reasons for seeking medical care. Population data indicate that the practice of physical activity improves gastrointestinal motility.

Objective: This study evaluated the bowel frequency and symptoms of constipation and their relationship with the level of physical activity in patients with and without Chagas disease. Read More

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http://dx.doi.org/10.1590/S0004-2803.202000000-30DOI Listing
December 2019

The use of the CALL Risk Score for predicting mortality in Brazilian heart failure patients.

ESC Heart Fail 2020 Jul 1. Epub 2020 Jul 1.

Postgraduate Division, São José do Rio Preto Medical School, São José do Rio Preto, São Paulo, Brazil.

Aims: This study aimed to develop and validate a simple method for predicting long-term all-cause mortality in ambulatory patients with chronic heart failure (CHF) residing in an area where Chagas disease is endemic, which will be important not only for patients living in Latin America but also to those living in developed non-endemic countries.

Methods And Results: A total of 677 patients with a wide spectrum of aetiologies for left ventricular systolic dysfunction and receiving optimized evidence-based treatment for CHF were prospectively followed for approximately 11 years. We established a risk score using Cox proportional hazard regression models. Read More

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http://dx.doi.org/10.1002/ehf2.12770DOI Listing

Repositioning of leishmanicidal [1,2,3]Triazolo[1,5-a]pyridinium salts for Chagas disease treatment: Trypanosoma cruzi cell death involving mitochondrial membrane depolarisation and Fe-SOD inhibition.

Parasitol Res 2020 Jul 1. Epub 2020 Jul 1.

Department of Parasitology, Instituto de Investigación Biosanitaria (ibs. Granada), Hospitales Universitarios de Granada/University of Granada, Severo Ochoa s/n, 18071, Granada, Spain.

Trypanosomatidae is a family of unicellular parasites belonging to the phylum Euglenozoa, which are causative agents in high impact human diseases such as Leishmaniasis, Chagas disease and African sleeping sickness. The impact on human health and local economies, together with a lack of satisfactory chemotherapeutic treatments and effective vaccines, justifies stringent research efforts to search for new disease therapies. Here, we present in vitro trypanocidal activity data and mode of action data, repositioning leishmanicidal [1,2,3]Triazolo[1,5-a]pyridinium salts against Trypanosoma cruzi, the aetiological agent of Chagas disease. Read More

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http://dx.doi.org/10.1007/s00436-020-06779-0DOI Listing

Venom alkaloids against Chagas disease parasite: search for effective therapies.

Sci Rep 2020 Jun 30;10(1):10642. Epub 2020 Jun 30.

Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.

Chagas disease is an important disease affecting millions of patients in the New World and is caused by a protozoan transmitted by haematophagous kissing bugs. It can be treated with drugs during the early acute phase; however, effective therapy against the chronic form of Chagas disease has yet to be discovered and developed. We herein tested the activity of solenopsin alkaloids extracted from two species of fire ants against the protozoan parasite Trypanosoma cruzi, the aetiologic agent of Chagas disease. Read More

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http://dx.doi.org/10.1038/s41598-020-67324-8DOI Listing

Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019.

Pathogens 2020 Jun 27;9(7). Epub 2020 Jun 27.

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Avenida Brasil 4365, Rio de Janeiro 21040-900, Brazil.

Rotavirus A (RVA) vaccines succeeded in lowering the burden of acute gastroenteritis (AGE) worldwide, especially preventing severe disease and mortality. In 2019, Brazil completed 13 years of RVA vaccine implementation (Rotarix™) within the National Immunization Program (NIP), and as reported elsewhere, the use of Rotarix™ in the country has reduced childhood mortality and morbidity due to AGE. Even though both marketed vaccines are widely distributed, the surveillance of RVA causing AGE and the monitoring of circulating genotypes are important tools to keep tracking the epidemiological scenario and vaccines impact. Read More

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http://dx.doi.org/10.3390/pathogens9070515DOI Listing

Autochthonous Chagas Disease in the United States: How Are People Getting Infected?

Am J Trop Med Hyg 2020 Jun 29. Epub 2020 Jun 29.

Division of Infectious Diseases, Department of Medicine, University of Arizona College of Medicine, Tucson, Arizona.

In the United States, Chagas disease is diagnosed in less than 1% of the estimated > 300,000 people who have the disease. However, the actual prevalence remains unknown, and these estimates may be wide of the mark (too high or too low). The greater part of those living with the disease acquired the infection in an endemic region of Latin America, but autochthonous transmission in the United States is increasingly being described. Read More

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http://dx.doi.org/10.4269/ajtmh.19-0733DOI Listing

Efficacy of Novel Pyrazolone Phosphodiesterase Inhibitors in Experimental Mouse Models of .

Antimicrob Agents Chemother 2020 Jun 29. Epub 2020 Jun 29.

Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

Pyrazolones are heterocyclic compounds with interesting biological properties. Some derivatives inhibit phosphodiesterases (PDEs) and thereby increase the cellular concentration of cyclic AMP (cAMP), which plays a vital role in the control of metabolism in eukaryotic cells, including the protozoan , the etiological agent of Chagas disease (CD), a major Neglected Tropical Disease. phenotypic screening identified a 4-bromophenyl-dihydropyrazole dimer as anti- hit and 17 novel pyrazolone analogues with variations on the phenyl ring were investigated in a panel of phenotypic laboratory models. Read More

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http://dx.doi.org/10.1128/AAC.00414-20DOI Listing

Asymptomatic Strongyloidiasis among Latin American Migrants in Spain: A Community-Based Approach.

Pathogens 2020 Jun 24;9(6). Epub 2020 Jun 24.

Clinica Medicine Department, University Miguel Hernández de Elche, 03550 Sant Joan d'Alacant, Alicante, Spain.

infection is frequently underdiagnosed since many infections remain asymptomatic.

Aim: To estimate the prevalence and characteristics of asymptomatic infection in Latin American migrants attending a community-based screening program for Chagas disease in Spain.

Methodology: Three community-based Chagas disease screening campaigns were performed in Alicante (Spain) in 2016, 2017, and 2018. Read More

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http://dx.doi.org/10.3390/pathogens9060511DOI Listing

Voriconazole-Benznidazole Combination Treatment for Chagas Disease.

Acta Trop 2020 Jun 26:105606. Epub 2020 Jun 26.

Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños "Dr. Ricardo Gutiérrez". Ministerio de Salud. GCBA, Buenos Aires, Argentina; Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas (IMIPP), CONICET-GCBA, Buenos Aires, Argentina; Division of Pediatric Clinical Pharmacology, Department of Pediatrics, Schulich School of Medicine & Dentistry, University of Western Ontario. Electronic address:

Combination therapy has been proposed as an ideal strategy to reduce drug toxicity and improve treatment efficacy in Chagas disease. Previously, we demonstrated potent in vivo anti-Trypanosoma cruzi activity of voriconazole. In this work, we aimed to study the synergistic effect of voriconazole (VCZ) and benznidazole (BZ) both in vitro and in vivo models of T. Read More

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http://dx.doi.org/10.1016/j.actatropica.2020.105606DOI Listing

Impact of the social context on the prognosis of Chagas disease patients: Multilevel analysis of a Brazilian cohort.

PLoS Negl Trop Dis 2020 Jun 29;14(6):e0008399. Epub 2020 Jun 29.

Graduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.

The present study aims to investigate how the social context contributes to the prognosis of Chagas disease (CD). This is a multilevel study that considered individual and contextual data. Individual data came from a Brazilian cohort study that followed 1,637 patients who lived in 21 municipalities to which CD is endemic, over two years. Read More

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http://dx.doi.org/10.1371/journal.pntd.0008399DOI Listing

EIF2α phosphorylation is regulated in intracellular amastigotes for generation of infective Trypanosoma cruzi trypomastigote forms.

Cell Microbiol 2020 Jun 29:e13243. Epub 2020 Jun 29.

Departmento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.

Trypanosomatids regulate gene expression mainly at the post-transcriptional level through processing, exporting and stabilizing mRNA and control of translation. In most eukaryotes, protein synthesis is regulated by phosphorylation of eukaryotic initiation factor 2 (eIF2) at serine 51. Phosphorylation halts overall translation by decreasing availability of initiator tRNA to form translating ribosomes. Read More

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http://dx.doi.org/10.1111/cmi.13243DOI Listing

DH82 Canine and RAW264.7 Murine Macrophage Cell Lines Display Distinct Activation Profiles Upon Interaction With .

Front Cell Infect Microbiol 2020 12;10:247. Epub 2020 Jun 12.

Instituto de Veterinária, Universidade Federal Rural Do Rio de Janeiro, Seropédica, Brazil.

Leishmaniasis is an anthropozoonotic disease, and dogs are considered the main urban reservoir of the parasite. Macrophages, the target cells of ., play an important role during infection. Read More

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http://dx.doi.org/10.3389/fcimb.2020.00247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303514PMC

Paradoxical Effect on Infection.

Iran J Parasitol 2020 Apr-Jun;15(2):223-232

Pathology Laboratory, Department of Morphology, Biological Sciences National School, National Polytechnic Institute, Mexico City, Mexico.

Background: There are only two anti-trypanocidal drugs available, both have a lot of side effects. This is the pioneer study designed to evaluate the effect in -infected mice and macrophages.

Methods: was administered in vivo, and in vitro (120μL/mL; 200 μL/mL; 500 μL/mL; 852 μL/mL) as prophylaxis, and treatment. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311822PMC

Comparison of the computed tomography findings in COVID-19 and other viral pneumonia in immunocompetent adults: a systematic review and meta-analysis.

Eur Radiol 2020 Jun 27. Epub 2020 Jun 27.

Medical Imaging Research Lab, LABIMED, Department of Radiology, Pavilhão Pereira Filho Hospital, Irmandade Santa Casa de Misericórdia de Porto Alegre, Av. Independência, 75, Porto Alegre, 90020160, Brazil.

Objectives: To compare the chest computed tomography (CT) findings of coronavirus disease 2019 (COVID-19) to other non-COVID viral pneumonia.

Methods: MEDLINE, EMBASE, and Cochrane databases were searched through April 04, 2020, for published English language studies. Studies were eligible if they included immunocompetent patients with up to 14 days of viral pneumonia. Read More

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http://dx.doi.org/10.1007/s00330-020-07018-xDOI Listing

Chagas Disease. Epidemiology and Barriers to Treatment.

Authors:
Roger M Mills

Am J Med 2020 Jun 24. Epub 2020 Jun 24.

Renova Therapeutics, Inc, 8345 Granite Ct, Dexter, MI 48130, United States. Electronic address:

Chronic human infection by the protozoan parasite Trypanosoma cruzi, known as Chagas disease, results in heart failure and death in 20 to 30% of affected individuals. Recognition and treatment of the infection are difficult. Disease control requires elimination of the vector, the reduviid bug, that infests poor quality housing in endemic areas. Read More

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http://dx.doi.org/10.1016/j.amjmed.2020.05.022DOI Listing

Gene expression to contaminated or not contaminated Triatoma dimidiata faeces with Trypanosoma cruzi in vivo and in vitro assay.

Ann Parasitol 2020 (66)

LADISER Immunology and Molecular Biology, Faculty of Chemical Sciences, Veracruzana University, Prolongación Oriente 6, No.1009, Col. Rafael Alvarado, 94340 Orizaba, Veracruz, Mexico.

Triatoma dimidiata is considered one of the most important vectors in the spread of Chagas disease in Mesoamerica. Hematophagous insects may deposit faeces contaminated or not with Trypanosoma cruzi on the surface of the skin during the feeding process. In endemic areas humans can be in contact several times to this fluid before get infection. Read More

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http://dx.doi.org/10.17420/ap6602.255DOI Listing
January 2020

TLR4 agonist protects against Trypanosoma cruzi acute lethal infection by decreasing cardiac parasite burdens.

Parasite Immunol 2020 Jun 26:e12769. Epub 2020 Jun 26.

Texas Children's Hospital Center for Vaccine Development, Department of Pediatrics and National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America.

E6020 is a synthetic agonist of Toll-like receptor-4 (TLR4). The purpose of this study was to evaluate the effect of different doses of E6020-SE on T. cruzi specific immune responses and its ability to confer protection against acute lethal infection in mice. Read More

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http://dx.doi.org/10.1111/pim.12769DOI Listing

Discovery of highly potent and selective antiparasitic new oxadiazole and hydroxy-oxindole small molecule hybrids.

Eur J Med Chem 2020 May 16;201:112418. Epub 2020 May 16.

Laboratory of Synthesis of Natural Products and Drugs, Institute of Chemistry, University of Campinas, PO Box 6154, 13083-970, Campinas, SP, Brazil. Electronic address:

A series of highly active hybrids were discovered as novel antiparasitic agents. Two heterocyclic scaffolds (1,2,4-oxadiazole and 3-hydroxy-2-oxindole) were linked, and the resulting compounds showed in vitro activities against intracellular amastigotes of two protozoan parasites, Trypanosoma cruzi and Leishmania infantum. Their cytotoxicity was assessed using HFF-1 fibroblasts and HepG2 hepatocytes. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112418DOI Listing
May 2020
3.447 Impact Factor

Histone deacetylases as targets for the treatment of neurodegenerative disorders: Challenges and future opportunities.

Med Res Rev 2020 Jun 26. Epub 2020 Jun 26.

Laboratório de Avaliação e Síntese de Substâncias Bioativas (LASSBio), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Despite the applicability of histone deacetylase inhibitors (HDACis) for cancer treatment, several works in the literature have shown that these inhibitors can be used in several other diseases, such as neurodegenerative diseases (NDs). This review begins by discussing the signaling pathways of HDACs, focused on the context of NDs, presenting a discussion about the pharmacophoric features of HDACis and crystal structure analysis and discussing interesting case studies from the literature about the development of HDACis. Additionally, a discussion about the consequences of isoform-selective inhibition vs pan-HDACis on neurotoxic effects and clinical trial investigations of HDACis for NDs is also presented. Read More

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http://dx.doi.org/10.1002/med.21701DOI Listing

The Parasitic Intracellular Lifestyle of Trypanosomatids: Parasitophorous Vacuole Development and Survival.

Front Cell Dev Biol 2020 10;8:396. Epub 2020 Jun 10.

Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

The trypanosomatid (protozoan) parasites and spp. are causative agents of Chagas disease and , respectively. They display high morphological plasticity, are capable of developing in both invertebrate and vertebrate hosts, and are the only trypanosomatids that can survive and multiply inside mammalian host cells. Read More

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http://dx.doi.org/10.3389/fcell.2020.00396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297907PMC

Stop, think SCORTCH: rethinking the traditional 'TORCH' screen in an era of re-emerging syphilis.

Arch Dis Child 2020 Jun 25. Epub 2020 Jun 25.

Department of Paediatric Infectious Diseases, Imperial College Healthcare NHS Trust, London, UK.

Background: The epidemiology of congenital infections is ever changing, with a recent resurgence in syphilis infection rates seen in the UK. Identification of congenital infection is often delayed; early recognition and management of congenital infections is important. Testing modalities and investigations are often limited, leading to missed diagnostic opportunities. Read More

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http://dx.doi.org/10.1136/archdischild-2020-318841DOI Listing

Cerebrospinal fluid irisin correlates with amyloid-β, BDNF, and cognition in Alzheimer's disease.

Alzheimers Dement (Amst) 2020 21;12(1):e12034. Epub 2020 Jun 21.

Institute of Medical Biochemistry Leopoldo de Meis Federal University of Rio de Janeiro Cidade Universitaria - Rio de Janeiro Rio de Janeiro Brazil.

Introduction: Irisin is a novel hormone originally identified for its role as a regulator of peripheral metabolism and recently found to protect synapses and rescue memory in mouse models of Alzheimer's disease (AD). However, whether and how cerebrospinal fluid (CSF) irisin varies in relation to canonical AD biomarkers and cognition in humans remains unknown.

Methods: We determined CSF levels of irisin and brain-derived neurotrophic factor (BDNF) and examined their correlations with CSF amyloid beta (Aβ), total tau, and Mini-Mental State Exam (MMSE) scores in a cohort comprising AD patients ( = 14) and non-demented controls (NDC; = 25). Read More

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http://dx.doi.org/10.1002/dad2.12034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306518PMC

Mining Sudanese Medicinal Plants for Antiprotozoal Agents.

Front Pharmacol 2020 9;11:865. Epub 2020 Jun 9.

Faculty of Pharmacy, University of Khartoum, Khartoum, Sudan.

Neglected tropical diseases are major health hazards in developing countries. Annually, up to 30 million people are affected by either Chagas disease, African trypansomiasis or leishmaniasis, and more than 200 million by malaria. Most of the currently available drugs have drawbacks in terms of toxicity, limited oral availability, development of resistance, or non-affordability. Read More

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http://dx.doi.org/10.3389/fphar.2020.00865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295952PMC

Endophytic Fungi In The Fight Against Neglected Tropical Diseases.

Mini Rev Med Chem 2020 Jun 24. Epub 2020 Jun 24.

Institute of Exact and Natural Sciences, Federal University of Mato Grosso, Rondonópolis. Brazil.

Neglected tropical diseases are a serious global public health problem and they are one of the main causes of mortality and morbidity, especially in underdeveloped countries. These diseases have several implications for health and they are considered a priority in global eradication programs for disease control. The aim of this mini-review is to report recent studies on the fight against neglected diseases, namely dengue fever, chikungunya, zika, malaria protozoa, Chagas disease, leishmaniasis, schistosomiasis helminths, filariasis, and tuberculosis bacteria using extracts and isolated substances of endophytic fungi based on their bioactivity profiles in relation to these diseases. Read More

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http://dx.doi.org/10.2174/1389557520666200624193300DOI Listing

Awareness of Chagas disease and socioeconomic characteristics of Bolivian immigrants living in Sao Paulo, Brazil.

Rev Inst Med Trop Sao Paulo 2020 17;62:e39. Epub 2020 Jun 17.

Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil.

In this study which is part of a research project on Chagas disease (CD) among Bolivian immigrants in Sao Paulo, we describe socioeconomic characteristics, knowledge of CD and implications for acess to health care. We applied a structured questionnaire to a sample of 472 Bolivian adults (> 18 years) living in Sao Paulo and enrolled at the Barra Funda School Health Center. Participants' median age was 28. Read More

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http://dx.doi.org/10.1590/S1678-9946202062039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304264PMC

Efficacy of the 7-chloro-4-(3-hydroxy-benzilidenehydrazo)quinoline derivative against infection caused by Leishmania amazonensis.

Rev Soc Bras Med Trop 2020 22;53:e20200091. Epub 2020 Jun 22.

Universidade Federal de Juiz de Fora, Campus Universitário, Departamento de Parasitologia, Microbiologia e Imunologia, Juiz de Fora, MG, Brasil.

Introduction: The drugs currently available for leishmaniasis treatment have major limitations.

Methods: In vitro and in vivo studies were performed to evaluate the effect of a quinoline derivative, Hydraqui (7-chloro-4-(3-hydroxy-benzilidenehydrazo)quinoline, against Leishmania amazonensis. In silico analyses of absorption, distribution, metabolism, excretion, and toxicity (ADMET) parameters were performed. Read More

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http://dx.doi.org/10.1590/0037-8682-0091-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310358PMC

Cutaneous leishmaniasis: a pathological study of 360 cases with special emphasis on the contribution of immunohistochemistry and polymerase chain reaction to diagnosis.

J Cutan Pathol 2020 Jun 23. Epub 2020 Jun 23.

Department of Pathology, Hospital Universitario de Fuenlabrada, Madrid, Spain.

Background: Traditional methods for the diagnosis of leishmaniasis yield poor sensitivity, which limits its effectiveness in lesions with a low parasite burden.

Methods: Retrospective pathologic study of 360 cases of cutaneous leishmaniasis and analysis of the different diagnostic methods used.

Results: In 93% of the lesions, histopathology showed a dense and diffuse inflammatory infiltrate, consisting of lymphocytes, histiocytes and plasma cells, which occupied the superficial and mid dermis and variably extended to deep dermis and superficial subcutis (standard pattern). Read More

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http://dx.doi.org/10.1111/cup.13785DOI Listing

Nanoparticle-based thymulin gene therapy therapeutically reverses key pathology of experimental allergic asthma.

Sci Adv 2020 Jun 10;6(24):eaay7973. Epub 2020 Jun 10.

Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Despite long-standing efforts to enhance care for chronic asthma, symptomatic treatments remain the only option to manage this highly prevalent and debilitating disease. We demonstrate that key pathology of allergic asthma can be almost completely resolved in a therapeutic manner by inhaled gene therapy. After the disease was fully and stably established, we treated mice intratracheally with a single dose of thymulin-expressing plasmids delivered via nanoparticles engineered to have a unique ability to penetrate the airway mucus barrier. Read More

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http://dx.doi.org/10.1126/sciadv.aay7973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286682PMC

Geospatial tools and Chagas control: a critical comment on the paper of Weinberg et al., Evaluation and planning of Chagas control activities using geospatial tools. Geospat Health 2019;14:229-238.

Authors:
David E Gorla

Geospat Health 2020 Jun 22;15(1). Epub 2020 Jun 22.

Instituto de Diversidad y Ecología Animal, CONICET - Universidad Nacional de Córdoba.

The contribution of the geospatial tools to the vector control activities of Chagas disease is very clear for the academic and technology-prone communities, but not so much for the state-dependent health agents (national or provincial) that are ultimately responsible for disease control in Latin America. (.. Read More

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http://dx.doi.org/10.4081/gh.2020.901DOI Listing

CXCR3 chemokine receptor contributes to specific CD8+ T cell activation by pDC during infection with intracellular pathogens.

PLoS Negl Trop Dis 2020 Jun 23;14(6):e0008414. Epub 2020 Jun 23.

Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, Brazil.

Chemokine receptor type 3 (CXCR3) plays an important role in CD8+ T cells migration during intracellular infections, such as Trypanosoma cruzi. In addition to chemotaxis, CXCR3 receptor has been described as important to the interaction between antigen-presenting cells and effector cells. We hypothesized that CXCR3 is fundamental to T. Read More

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http://dx.doi.org/10.1371/journal.pntd.0008414DOI Listing

Combined analysis of circular RNA and micro RNA profiles and interactions in patients with diabetic foot and diabetes mellitus.

Int Wound J 2020 Jun 23. Epub 2020 Jun 23.

Department of General Surgery, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

In order to elucidate the pathogenesis and explore new biomarkers for diabetes and diabetic foot (DF), an analysis using RNA sequencing affords broader insights into gene expression regulatory networks in DF. To better explore the molecular basis of DF, we carried out an analysis of circular RNA (circRNA) and messenger RNA (mRNA) expression profiles of serum samples from DF patients and diabetes mellitus (DM) patients. The potential roles and interactions of differentially expressed circRNAs and mRNAs were classified by gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Read More

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http://dx.doi.org/10.1111/iwj.13420DOI Listing

New scheme of intermittent benznidazole administration in patients chronically infected with : Clinical, parasitological and serological assessment after three years of follow-up.

Antimicrob Agents Chemother 2020 Jun 22. Epub 2020 Jun 22.

Hospital Interzonal General de Agudos "Eva Perón"; San Martín, Buenos Aires, Argentina.

In a pilot study, we showed that intermittent administration of benznidazole in chronic Chagas disease patients resulted in a low rate of treatment suspension and therapeutic failure, as assessed by qPCR at the end of treatment. Herein, a three-year post-treatment follow-up study of the same cohort of patients is presented. The treatment scheme consisted of 12 doses of benznidazole at 5 mg/kg/day in two daily doses every 5 days. Read More

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http://dx.doi.org/10.1128/AAC.00439-20DOI Listing

Replication origin location might contribute to genetic variability in Trypanosoma cruzi.

BMC Genomics 2020 Jun 22;21(1):414. Epub 2020 Jun 22.

Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, Brazil.

Background: DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown.

Results: Here we have mapped sites of DNA replication initiation across the T. Read More

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http://dx.doi.org/10.1186/s12864-020-06803-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310030PMC

The 14th of April, Past and Present.

Trop Med Infect Dis 2020 Jun 18;5(2). Epub 2020 Jun 18.

Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro 21040-360, Brazil.

In May 2019, the World Health Organization established the "World Chagas Disease Day", to be celebrated on the 14th of April [... Read More

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http://dx.doi.org/10.3390/tropicalmed5020100DOI Listing

Access to Chagas disease treatment in the United States after the regulatory approval of benznidazole.

PLoS Negl Trop Dis 2020 Jun 22;14(6):e0008398. Epub 2020 Jun 22.

Department of Global Health and Population, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America.

Approximately 300,000 persons in the United States (US) are infected with Trypanosoma cruzi, the protozoan that causes Chagas disease, but less than 1% are estimated to have received antiparasitic treatment. Benznidazole was approved by the US Food and Drug Administration (FDA) for treatment of T. cruzi infection in 2017 and commercialized in May 2018. Read More

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http://dx.doi.org/10.1371/journal.pntd.0008398DOI Listing

Molecular data supports monophyly of Triatoma dispar complex within genus Triatoma.

Infect Genet Evol 2020 Jun 18:104429. Epub 2020 Jun 18.

Center for Research on Health in Latin America (CISeAL), School of Biological Sciences, Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del Ecuador, Calle Pambahacienda s/n y San Pedro del Valle, Campus Nayón, Quito, Ecuador; Institut de Recherche pour le Développement (IRD), UMR INTERTRYP IRD-CIRAD, University of Montpellier, TA A-17/G, International Campus in Baillarguet, Montpellier, France. Electronic address:

The genus Triatoma contains numerous species, principal or secondary vectors of Chagas disease, which have been included in the three main lineages of Triatomini tribe based on morphological and biogeographical characteristics: North American, South American, and T. dispar complex. The three members of the T. Read More

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http://dx.doi.org/10.1016/j.meegid.2020.104429DOI Listing

Emerging trends in COVID-19 treatment: learning from inflammatory conditions associated with cellular therapies.

Cytotherapy 2020 May 7. Epub 2020 May 7.

Center for Cancer and Immunology Research, Center for Cancer and Blood Disorders, Children's National Hospital and the George Washington University Cancer Center, George Washington University, Washington, DC, USA.

Coronavirus disease 2019 (SARS-CoV2) is an active global health threat for which treatments are desperately being sought. Even though most people infected experience mild to moderate respiratory symptoms and recover with supportive care, certain vulnerable hosts develop severe clinical deterioration. While several drugs are currently being investigated in clinical trials, there are currently no approved treatments or vaccines for COVID-19 and hence there is an unmet need to explore additional therapeutic options. Read More

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http://dx.doi.org/10.1016/j.jcyt.2020.04.100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252029PMC

Effect of Posaconazole in an in vitro model of cardiac fibrosis induced by Trypanosoma cruzi.

Mol Biochem Parasitol 2020 Jun 18:111283. Epub 2020 Jun 18.

Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. Electronic address:

Posaconazole (POS) is an inhibitor of ergosterol biosynthesis in clinical use for treating invasive fungal infections. POS has potent and selective anti-Trypanosoma cruzi activity and has been evaluated as a possible treatment for Chagas disease. Microtissues are a 3D culture system that has been shown to reproduce better tissue architecture and functionality than cell cultures in monolayer (2D). Read More

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http://dx.doi.org/10.1016/j.molbiopara.2020.111283DOI Listing

Factors associated with absenteeism of nursing professionals in university outpatient clinics in Brazil.

J Nurs Manag 2020 Jun 21. Epub 2020 Jun 21.

Escola de Enfermagem Anna Nery/EEAN, Universidade Federal do Rio de Janeiro/UFRJ. Rio de Janeiro, Rio de Janeiro, Brasil.

Objective: To analyze the sociodemographic, occupational and health profile of nursing professionals working in university outpatient services and their relationship with absenteeism.

Background: The organization and working conditions in outpatient services may be directly related to the illness and absenteeism in the nursing profession.

Method: Analytical cross-sectional study conducted in 11 outpatient clinics in the city of Rio de Janeiro/Brazil with nursing professionals. Read More

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http://dx.doi.org/10.1111/jonm.13073DOI Listing

Challenges in Chagas Disease Drug Development.

Molecules 2020 Jun 17;25(12). Epub 2020 Jun 17.

Department of Infection Biology, London School of Hygiene and Tropical Medicine Keppel Street, London WC1E 7HT, UK.

The protozoan parasite causes Chagas disease, an important public health problem throughout Latin America. Current therapeutic options are characterised by limited efficacy, long treatment regimens and frequent toxic side-effects. Advances in this area have been compromised by gaps in our knowledge of disease pathogenesis, parasite biology and drug activity. Read More

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http://dx.doi.org/10.3390/molecules25122799DOI Listing

Computational Drug Repositioning for Chagas Disease Using Protein-Ligand Interaction Profiling.

Int J Mol Sci 2020 Jun 16;21(12). Epub 2020 Jun 16.

Laboratorio de Biotecnología Farmacéutica, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa 88710, Mexico.

Chagas disease, caused by (), affects nearly eight million people worldwide. There are currently only limited treatment options, which cause several side effects and have drug resistance. Thus, there is a great need for a novel, improved Chagas treatment. Read More

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http://dx.doi.org/10.3390/ijms21124270DOI Listing

Theoretical studies and NMR assay of coumarins and neoflavanones derivatives as potential inhibitors of acetylcholinesterase.

Comput Biol Chem 2020 May 29;87:107293. Epub 2020 May 29.

Laboratório Integrado de Biologia Computacional e Pesquisa em Ciências Farmacêuticas, Instituto de Biodiversidade e Sustentabilidade (NUPEM/UFRJ), Universidade Federal do Rio de Janeiro, Rua São José do Barreto 764, 27965-045 Macaé, RJ, Brazil. Electronic address:

Currently Alzheimer's disease (AD) is a devastating neurological disorder that mainly affects the elderly. The treatment of AD has as main objective to increase the levels of ACh in the synaptic cleft by inhibiting the cholinesterase enzymes, which are responsible for the degradation of ACh. Twenty one synthesized coumarins and neoflavanones (4-arylcoumarins) and theoretical studies were used to select the most promising ligands for in vitro experimental studies by Nuclear Magnetic Resonance. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2020.107293DOI Listing

Protective effect of fungal extracellular vesicles against murine candidiasis.

Cell Microbiol 2020 Jun 18:e13238. Epub 2020 Jun 18.

Laboratório de Glicobiologia de Eucariotos, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Extracellular vesicles (EVs) are lipid bilayered compartments released by virtually all living cells, including fungi. Among the diverse molecules carried by fungal EVs, a number of immunogens, virulence factors and regulators have been characterized. Within EVs, these components could potentially impact disease outcomes by interacting with the host. Read More

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http://dx.doi.org/10.1111/cmi.13238DOI Listing

Planning new Trypanosoma cruzi CYP51 inhibitors using QSAR studies.

Mol Divers 2020 Jun 16. Epub 2020 Jun 16.

Programa de Pós-Graduação em Bioinformática, Faculdade de Farmácia, Universidade Federal do Rio Grande do Norte, UFRN, Rua Gen. Gustavo Cordeiro de Faria, S/N - Petrópolis, Natal, RN, 59012-570, Brazil.

Chagas disease kills over 10,000 people per year, and approximately 8 million people are infected by Trypanosoma cruzi. The reference drug for treatment of the disease, benznidazole, is the same since the 70s. In recent years, many CYP51 inhibitors were tested against this parasite's target. Read More

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http://dx.doi.org/10.1007/s11030-020-10113-2DOI Listing

Chagas disease in Virgem da Lapa, Minas Gerais, Brazil: left ventricle aneurysm and the risk of death in the 24-year interval.

Mem Inst Oswaldo Cruz 2020 12;115:e200056. Epub 2020 Jun 12.

Fundação Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Pesquisa Clínica em Doença de Chagas, Rio de Janeiro, RJ, Brasil.

Background: Left ventricular aneurysm (LVA) is indicator of high morbidity in Chagas' disease. A cross-sectional study performed identified LVA in 18.8% of the chronic chagasic patients (CCP). Read More

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http://dx.doi.org/10.1590/0074-02760200056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292011PMC

The Functional Characterization of TcMyoF Implicates a Family of Cytostome-Cytopharynx Targeted Myosins as Integral to the Endocytic Machinery of Trypanosoma cruzi.

mSphere 2020 Jun 17;5(3). Epub 2020 Jun 17.

Department of Cellular Biology, Center for Tropical and Emerging Global Diseases (CTEGD), University of Georgia, Athens, Georgia, USA

Of the pathogenic trypanosomatids, alone retains an ancient feeding apparatus known as the cytotome-cytoharynx omplex (SPC) that it uses as its primary mode of endocytosis in a manner akin to its free-living kinetoplastid relatives who capture and eat bacterial prey via this endocytic organelle. In a recent report, we began the process of dissecting how this organelle functions by identifying the first SPC-specific proteins in Here, we continued these studies and report on the identification of the first enzymatic component of the SPC, a previously identified orphan myosin motor (MyoF) specifically targeted to the SPC. We overexpressed MyoF as a dominant-negative mutant, resulting in parasites that, although viable, were completely deficient in measurable endocytosis To our surprise, however, a full deletion of MyoF demonstrated only a decrease in the overall rate of endocytosis, potentially indicative of redundant myosin motors at work. Read More

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http://dx.doi.org/10.1128/mSphere.00313-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300353PMC

HTLV-I induces lesions in the pulmonary system: A systematic review.

Life Sci 2020 Jun 15;256:117979. Epub 2020 Jun 15.

Center for Tropical Medicine, Federal University of Pará, Belém, PA, Brazil; Center for Biological and Health Sciences, State University of Pará, Belém, PA, Brazil; Evandro Chagas Institute, Ministry of Health, Ananindeua, Belém, PA, Brazil. Electronic address:

This study analyzed the relationship between infection by human T-cell lymphotropic virus type 1 (HTLV-1) and changes in the pulmonary system. Cohort and case-control study models that analyzed a causal association between HTLV-1 and changes in the pulmonary system were considered. There were no restrictions on language and publication period. Read More

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http://dx.doi.org/10.1016/j.lfs.2020.117979DOI Listing

Urbanisation, risk stratification and house infestation with a major vector of Chagas disease in an endemic municipality of the Argentine Chaco.

Parasit Vectors 2020 Jun 18;13(1):316. Epub 2020 Jun 18.

Laboratorio de Eco-Epidemiología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina.

Background: The occurrence of the major vectors of Chagas disease has historically been linked to poor rural housing, but urban or peri-urban infestations are increasingly being reported. We evaluated a simple risk index to detect houses infested with Triatoma infestans and tested whether house infestation and vector abundance increased across the urban-to-rural gradient in Avia Terai, an endemic municipality of the Argentine Chaco; whether the association between infestation and selected ecological determinants varied across the gradient; and whether urban and peri-urban infestations were associated with population settlement history.

Methods: We conducted a screening survey of house infestation in 2296 urban, peri-urban and rural dwellings to identify high-risk houses based on a simple index, and then searched for triatomines in all high-risk houses and in a systematic sample of low-risk houses. Read More

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http://dx.doi.org/10.1186/s13071-020-04182-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302373PMC