Biochem Biophys Res Commun 2021 Jun 21;567:201-207. Epub 2021 Jun 21.
NGK Spark Plug-AIST Healthcare Materials Cooperative Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), 2266-98 Anagahora, Shimoshidami, Moriyama-ku, Nagoya, 463-8560, Japan.
We previously reported an antibody (clone ID: FR9, IgM-κ) that recognizes the sialyl oligosaccharide Neu5Acα2,6Galβ1,4GlcNAc as an epitope on glycoproteins and glycolipids. In the present study, we developed an antibody (clone ID: AFR45, IgM-κ) that recognizes Neu5Acα2,3Galβ1,4GlcNAc/Glc as an epitope on glycoproteins and glycolipids and compared the nucleotide and amino acid sequences of the immunoglobulin gene variable regions with those of FR9. The heavy chain variable (V) regions of FR9 and AFR45 were encoded by different V gene segments, each of which was composed of a characteristic D gene segment. Read More