533 results match your criteria Cellular reprogramming[Journal]


Generation of Induced Pluripotent Stem Cells from a Female Patient with a Xq27.3-q28 Deletion to Establish Disease Models and Identify Therapies.

Cell Reprogram 2020 Jun 30. Epub 2020 Jun 30.

Department of Pathology, University of Florida College of Medicine, Gainesville, Florida, USA.

Since it is extremely difficult to establish an animal model for human chromosomal abnormalities, induced pluripotent stem cells (iPSCs) provide a powerful alternative to study underlying mechanisms of these disorders and identify potential therapeutic interventions. In this study we established iPSCs from a young girl with a hemizygous deletion of Xq27.3-q28 who exhibited global developmental delay and intellectual disability from early in infancy. Read More

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http://dx.doi.org/10.1089/cell.2020.0012DOI Listing

Development and Mitochondrial Gene Expression in Brown Brocket Deer () Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer.

Cell Reprogram 2020 Jun 19. Epub 2020 Jun 19.

Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University (UECE), Fortaleza, Brazil.

The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. The transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. Read More

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http://dx.doi.org/10.1089/cell.2019.0069DOI Listing

Hypoxia Enhances Mesenchymal Characteristics Maintenance of Buffalo Bone Marrow-Derived Mesenchymal Stem Cells.

Cell Reprogram 2020 Jun 21;22(3):167-177. Epub 2020 May 21.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, People's Republic of China.

Bone marrow-derived mesenchymal stem cells (BMSCs) from livestock are valuable resources for veterinary therapeutics and animal reproduction. Previous studies have shown that hypoxic conditions were beneficial in maintaining the mesenchymal feature of BMSCs. However, the effects of hypoxia on buffalo BMSCs (bBMSCs) remain unclear. Read More

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http://dx.doi.org/10.1089/cell.2019.0097DOI Listing

and Interspecies Chimera Assay Using Early Pig Embryos.

Cell Reprogram 2020 Jun 19;22(3):118-133. Epub 2020 May 19.

Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt, Germany.

Chimeric pigs harboring organs derived from human stem cells are promising for patient-specific regenerative therapies. Induced pluripotent stem cells (iPSCs) can contribute to all cell types of the fetus, including germline after injection into embryos. However, ethical concerns prohibit testing human iPSCs in chimera assays. Read More

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http://dx.doi.org/10.1089/cell.2019.0107DOI Listing

Roles of Exosomes from Mesenchymal Stem Cells in Treating Osteoarthritis.

Cell Reprogram 2020 Jun 4;22(3):107-117. Epub 2020 May 4.

East Hospital, Tongji University School of Medicine, Shanghai, China.

Exosomes are small extracellular vesicles (EVs) with a diameter of 50-150 nm that play important roles in cell-to-cell communication through transportation of proteins, microRNAs, lncRNAs, and mRNAs. Some components, such as miRNAs, have been proven to be involved in inflammation regulation. Osteoarthritis (OA) is a progressive disease resulting in articular cartilage degeneration and subchondral bone deficiency. Read More

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http://dx.doi.org/10.1089/cell.2019.0098DOI Listing
June 2020
1.788 Impact Factor

Effect of Aerobic and Resistance Training on Endothelial Progenitor Cells in Mice with Type 2 Diabetes.

Cell Reprogram 2020 Apr 21. Epub 2020 Apr 21.

Department of Nursing, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Since no study has explored whether exercise could improve impaired proliferation, migration, and angiogenesis of endothelial progenitor cells (EPCs) in animal models or humans with type 2 diabetes, we aimed to explore the effect of different models of exercise on EPC function and expression of caveolin-1, PI3K, and AKT in mice with type 2 diabetes. Male db/db mice (age: 8 weeks) with type 2 diabetes were subjected to aerobic training (AT), resistance training (RT), or combined aerobic and resistance training (AT+RT) 3 or 4 days/week. Mice in the control group remained sedentary with no specific training requirement. Read More

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http://dx.doi.org/10.1089/cell.2019.0063DOI Listing

Induction of Hepatic Regeneration in an Experimental Model Using Hepatocyte-Differentiated Mesenchymal Stem Cells.

Cell Reprogram 2020 Jun 3;22(3):134-146. Epub 2020 Apr 3.

Clinical and Chemical Pathology Department, Kasr Alainy Faculty of Medicine, Cairo University, Cairo, Egypt.

Mesenchymal stem cell (MSC)-based liver tissue engineering on nanofibrous scaffold holds great promise for cell-based therapy in liver injuries and end-stage liver failure treatments. MSCs were generated from umbilical cord blood. Hepatogenic differentiation was induced on two-dimensional (2D) and three-dimensional (3D) culture system and characterized by morphology, scanning electron microscopy, immunocytochemistry, and gene expression. Read More

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http://dx.doi.org/10.1089/cell.2019.0076DOI Listing
June 2020
1.788 Impact Factor

Melatonin Enhances the Development of Porcine Cloned Embryos by Improving DNA Methylation Reprogramming.

Cell Reprogram 2020 Jun 24;22(3):156-166. Epub 2020 Mar 24.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.

Incomplete DNA methylation reprogramming in cloned embryos leads to poor cloning efficiency. Melatonin has been proven to improve the development of cloned embryos, however, the role of melatonin during somatic cell nuclear transfer remains unclear. This work demonstrated that 10 M melatonin significantly enhanced the developmental progress, reduced the arrested rate before zygotic genome activation, and upregulated the blastocyst rate of cloned embryos. Read More

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http://dx.doi.org/10.1089/cell.2019.0103DOI Listing

Differentiation of Urine-Derived Induced Pluripotent Stem Cells to Neurons, Astrocytes, and Microvascular Endothelial Cells from a Diabetic Patient.

Cell Reprogram 2020 Jun 24;22(3):147-155. Epub 2020 Mar 24.

Department of Neurology, the Second Hospital of Dalian Medical University, Dalian City, China.

Complications of central nervous system in type 2 diabetes mellitus (T2DM) often lead to cognitive impairment and seriously affect the quality of life. However, there is no individualized disease model. Urine-derived stem cells can be an ideal source for generating human induced pluripotent stem cells (hiPSCs) and progenitors, as they are easily accessible, noninvasive, and universally available. Read More

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http://dx.doi.org/10.1089/cell.2019.0088DOI Listing

Intermediate Standstill Clones Trapped in the Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells.

Cell Reprogram 2020 Apr 17;22(2):99-105. Epub 2020 Mar 17.

Zhejiang University School of Medicine First Affiliated Hospital, Hangzhou, China.

Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lines originating from cells that have exited the normal reprogramming route at an early time point. Read More

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http://dx.doi.org/10.1089/cell.2019.0083DOI Listing

The Effects of Knockout on Pluripotency and Differentiation of Mouse Induced Pluripotent Stem Cells.

Cell Reprogram 2020 Apr 5;22(2):90-98. Epub 2020 Mar 5.

Department of Histology and Embryology, Harbin Medical University, Harbin, China.

Induced pluripotent stem cell (iPSC) technology refers to the reprogramming of terminally differentiated somatic cells into pluripotent stem cells by introducing specific transcription factors that are known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. In this study, we reprogrammed the primary fibroblasts isolated from the mice, which carry the Oct4-green fluorescent protein reporter, and employed wild-type littermates as a control to induce iPSCs, then knocked out by infecting with Cre virus at the cellular level. The pluripotency and self-renewal capacity of iPSCs were determined. Read More

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http://dx.doi.org/10.1089/cell.2019.0071DOI Listing

Advances in Isolation and Culture of Chicken Embryonic Stem Cells .

Cell Reprogram 2020 Apr 5;22(2):43-54. Epub 2020 Mar 5.

Department of Veterinary Medicine, College of Animal Science and Technology, Southwest University, Chongqing, China.

Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-term maintenance of cESCs cannot be realized now, which impedes the establishment of cESC line and limits their applications. Read More

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http://dx.doi.org/10.1089/cell.2019.0080DOI Listing

Effect of Downregulating the Hippo Pathway Members YAP1 and LATS2 Transcripts on Early Development and Gene Expression Involved in Differentiation in Porcine Embryos.

Cell Reprogram 2020 Apr 5;22(2):62-70. Epub 2020 Mar 5.

The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Japan.

In mouse development, differentiation of the inner cell mass (ICM) and trophectoderm (TE) during the transition from the morula to blastocyst stage is regulated by the Hippo pathway; however, the functions of the Hippo pathway in porcine embryogenesis have not been investigated. In the present study, we examined the gene expression patterns of the Hippo pathway members yes-associated protein 1 (YAP1) and large tumor suppressor 2 (LATS2) and the functions of these genes during porcine preimplantation development using RNA interference. Both and mRNA levels were shown high in the matured oocytes and 1-cell stage embryos and fell progressively with development. Read More

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http://dx.doi.org/10.1089/cell.2019.0082DOI Listing

Yes-Associated Protein and PDZ Binding Motif: A Critical Signaling Pathway in the Control of Human Pluripotent Stem Cells Self-Renewal and Differentiation.

Cell Reprogram 2020 Apr 3;22(2):55-61. Epub 2020 Mar 3.

Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Human pluripotent stem cells (hPSCs) can self-renew indefinitely to generate cells like themselves with a normal karyotype and differentiate into other types of cells when stimulated with a proper set of internal and external signals. hPSCs including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are an alternative approach toward stem cell biology, drug discovery, disease modeling, and regenerative medicine. hESCs are commonly derived from the inner cell mass of preimplantation embryos and can maintain their pluripotency in appropriate culture media. Read More

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http://dx.doi.org/10.1089/cell.2019.0084DOI Listing
April 2020
1.788 Impact Factor

Source and Follicular Fluid Treatment During the Maturation of Recipient Oocytes Affects the Development of Cloned Pig Embryo.

Cell Reprogram 2020 Apr 3;22(2):71-81. Epub 2020 Mar 3.

National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Pig cloning technique is valuable in agriculture, biomedicine, and life sciences. However, the full-term developmental efficiency of cloned pig embryos is only about 1%, which limits pig cloning application. The quality of recipient oocytes greatly affects the developmental competence of cloned pig embryos. Read More

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http://dx.doi.org/10.1089/cell.2019.0091DOI Listing

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Cell Reprogram 2020 Apr 3;22(2):82-89. Epub 2020 Mar 3.

First Hospital, Jilin University, Changchun, Jilin, China.

Mesenchymal stem/stromal cells (MSCs) have been identified in almost all adult human tissues and been used in numerous clinical trials for a variety of diseases. Studies have shown that MSCs would undergo cellular senescence when cultured over a long term, which is brought on by increased epigenetic modifications, including DNA methylation. However, the mechanism of MSCs senescence is not well studied. Read More

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http://dx.doi.org/10.1089/cell.2019.0060DOI Listing
April 2020
1.788 Impact Factor

Effects of PXD101 and Embryo Aggregation on the Development of Mouse Parthenogenetic Embryos.

Cell Reprogram 2020 Feb;22(1):14-21

College of Animal Science & Technology, Southwest University, Chongqing, P. R. China.

To improve the isolation efficiency of parthenogenetic embryonic stem cells (pESCs) in mice, it is necessary to optimize the method to increase developmental competence of mice parthenogenetic blastocysts. Therefore, this study aims to investigate an optimal method for the production of mouse parthenogenetic blastocysts and isolation of pESC colonies by comparing the effects of two methods: (1) the treatment of histone deacetylase inhibitor PXD101 before, during, or after parthenogenetic activation; (2) parthenogenetic embryo aggregation; and (3) their combination treatment. The results suggest that application of PXD101 treatment and embryo aggregation could both improve the development of mouse parthenogenetic blastocysts (50 nM PXD101 treated 4 hours during activation and further 4 hours after activation: 40. Read More

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http://dx.doi.org/10.1089/cell.2019.0038DOI Listing
February 2020

Reprogramming of Pluripotency-Specific microRNA Signatures Is Not Essential to Generate Inducible Pluripotent Stem Cells.

Cell Reprogram 2020 Feb;22(1):1-2

Cancer Biology and Genetics Program, Center for Cell Engineering, Center for Stem Cell Biology, Sloan-Kettering Institute, Cell and Developmental Biology Program, Weill Medical College of Cornell University, New York, New York.

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http://dx.doi.org/10.1089/cell.2019.0070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041330PMC
February 2020

Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells.

Cell Reprogram 2020 Feb;22(1):22-29

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning, Guangxi, China.

In this study, Squalene epoxidase () overexpression vector was transfected into bovine skeletal muscle-derived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of regulating meat quality through myogenesis. We initially profiled the expression of in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression of during cell proliferation and differentiation. Read More

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http://dx.doi.org/10.1089/cell.2019.0077DOI Listing
February 2020

Timing of the First Cleavage and Developmental Potential of Bovine Somatic Cell Nuclear Transfer Embryos Activated by Different Protocols.

Cell Reprogram 2020 Feb 31;22(1):36-42. Epub 2019 Dec 31.

Research and Education Faculty, Kochi University, Nankoku, Japan.

The objective of this study was to examine the relationship between the timing of the first cleavage and development of somatic cell nuclear transfer (SCNT) embryos produced by different activation protocols. SCNT embryos were activated with calcium ionophore A23187 and further treated with 6-dimethylaminopurine (DMAP group), cycloheximide (CHX group), or anisomycin (ANI group). The proportion of SCNT embryos that cleaved within 18 hours after activation was significantly higher in the DMAP group (20%) than that in the CHX and ANI groups (3% and 2%, respectively). Read More

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http://dx.doi.org/10.1089/cell.2019.0074DOI Listing
February 2020

Effect of Allogenic Bone Marrow Mesenchymal Stem Cell Transplantation on T Cells of Old Mice.

Cell Reprogram 2020 Feb 31;22(1):30-35. Epub 2019 Dec 31.

International Medical Plastic and Cosmetic Centre, China Meitan General Hospital, Beijing, China.

To evaluate age-related changes in T cells and stem cell-related genes in mice and the changes in T cells and stem cell-related genes after allogenic bone marrow mesenchymal stem cell (BMSC) transplantation, BALB/c mice were divided into young (2 months,  = 5) and old (20 months,  = 5) groups and 1 × 10 BMSCs from 3-week-old C57BL/6J mice were injected into the old mice ( = 5). T lymphocytes including CD3, CD8, CD8CD28, and CD8CD44CD62LSca-1 stem cell-like memory T cells from spleens were analyzed by flow cytometry. mRNA transcriptions of the tumor suppressor and the senescence inhibiting and stem cell-related , , , , , , , , and genes were analyzed by quantitative reverse transcription-polymerase chain reaction for comparison between young and old mice and old mice after BMSC application. Read More

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http://dx.doi.org/10.1089/cell.2019.0055DOI Listing
February 2020
1.788 Impact Factor

The Positive Effect of TET2 on the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells.

Cell Reprogram 2020 Feb 12;22(1):3-13. Epub 2019 Dec 12.

Department of Traumatic Orthopedics, Jining No. 1 People's Hospital, Jining, China.

This study aims to understand the possible effects of TET2 (ten-eleven translocation 2) on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (ADSCs). The human ADSCs were transfected with TET2 siRNAs. The osteogenesis-related genes were detected by quantitative real-time reverse transcription PCR (qRT-PCR), and the osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and Alizarin Red staining. Read More

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http://dx.doi.org/10.1089/cell.2019.0045DOI Listing
February 2020
1.788 Impact Factor

Dysfunction of MiR-148a-NRP1 Functional Axis Suppresses Osteogenic Differentiation of Periodontal Ligament Stem Cells Under Inflammatory Microenvironment.

Cell Reprogram 2019 12;21(6):314-322

Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, China.

Periodontitis is a chronic inflammatory disease that can lead to the loss of periodontal bone tissue. The osteogenic potential of periodontal ligament stem cells (PDLSCs) is significantly decreased in periodontitis microenvironment. However, the mechanism is still unclear. Read More

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http://dx.doi.org/10.1089/cell.2019.0026DOI Listing
December 2019

Adipose-Derived Stem Cells Inhibited the Proliferation of Bladder Tumor Cells by S Phase Arrest and Wnt/β-Catenin Pathway.

Cell Reprogram 2019 12;21(6):331-338

Department of Radiography, Renmin Hospital of Wuhan University, Wuhan, China.

Adipose-derived stem cells (ADSCs), which are present in most organs and tissues, were evaluated as a novel medium for stem cell therapy. In this study, we investigated the effects and underlying mechanisms of ADSCs in bladder tumor (BT) cells. SV-HUC, T24, and EJ cells were cultured with ADSCs and conditioned medium from ADSCs (ADSC-CM). Read More

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http://dx.doi.org/10.1089/cell.2019.0047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918853PMC
December 2019

Rosuvastatin Attenuates Myocardial Ischemia-Reperfusion Injury via Upregulating -Mediated Autophagy.

Cell Reprogram 2019 12 15;21(6):323-330. Epub 2019 Nov 15.

Department of Cardiovascular Medicine, Jingmen No.1 People's Hospital, Jingmen, China.

Myocardial diseases usually appear ischemic. Reperfusion therapy is one of the effective methods that can improve clinical therapeutic efficacy. However, reperfusion results in myocardial injury named I/R injury. Read More

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http://dx.doi.org/10.1089/cell.2018.0053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918854PMC
December 2019

Induction of Human Embryonic Stem Cells into the Midbrain Dopaminergic Neurons and Transplantation in Cynomolgus Monkey.

Cell Reprogram 2019 12 25;21(6):285-295. Epub 2019 Oct 25.

Embryo Engineering Lab, College of Animal Science & Technology, Southwest University, Chongqing, P R. China.

A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons . It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small molecules LDN (inhibitor of BMP [bone morphogenetic protein] pathway that inhibits BMP type I receptors ALK and ALK), SB431542 (inhibitor of TGF-β/Activin/Dodal pathway that inhibits ALK, ALK, and ALK), CHIR99021 (inhibitors of GSK-3 [glycogen synthase kinase 3]), and basic fibroblast growth factor (bFGF). The dopaminergic neurons were successfully induced at day 25 (tyrosine hydroxylase [TH] expressed) and at day 32 (TH highly expressed) with high purity (TH/Tuj1: 84. Read More

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http://dx.doi.org/10.1089/cell.2019.0037DOI Listing
December 2019

Comparison of Carcass Traits, Meat Quality, and Chemical Composition of Tissues from Progeny Derived from Cloned and Noncloned Pigs.

Cell Reprogram 2019 12 25;21(6):296-300. Epub 2019 Oct 25.

National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.

Systematic studies of progeny derived from somatic cell nuclear transferred animals in their biophysical and biochemical characters are critical in assessing the safety of this kind of food. In this study, we compared the carcass traits and meat quality of 12 cloned and noncloned pigs, respectively, and chemical composition of tissues of 6 cloned and noncloned pigs, respectively. The carcass trait parameters, including body weight, carcass straight length, loin-eye area, backfat thickness at 10th and 11th interface, and rib number, were tested, and carcass yield was calculated. Read More

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http://dx.doi.org/10.1089/cell.2019.0040DOI Listing
December 2019

Whole Blood Transcriptome Analysis for Lifelong Monitoring in Elite Sniffer Dogs Produced by Somatic Cell Nuclear Transfer.

Cell Reprogram 2019 12 18;21(6):301-313. Epub 2019 Oct 18.

National Institute of Animal Science, Rural Development Administration, Isero-myeon, Wanju-gun, Republic of Korea.

Reproductive cloning by somatic cell nuclear transfer (SCNT) is a valuable method to propagate service dogs with desirable traits because of higher selection rates in cloned dogs. However, incomplete reprogramming is a major barrier to SCNT, and the assessment of reprogramming is limited to preimplantation embryos and tissues from dead and/or adult tissue. Thus, lifelong monitoring in SCNT dogs can be useful to evaluate the SCNT service dogs for propagation. Read More

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http://dx.doi.org/10.1089/cell.2019.0056DOI Listing
December 2019

Successful Derivation of an Induced Pluripotent Stem Cell Line from a Genetically Nonpermissive Enhanced Green Fluorescent Protein-Transgenic FVB/N Mouse Strain.

Cell Reprogram 2019 10;21(5):270-284

Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India.

The embryonic stem cell line derivation from nonpermissive mouse strains is a challenging and highly inefficient process. The cellular reprogramming strategy provides an alternative route for generating pluripotent stem cell (PSC) lines from such strains. In this study, we successfully derived an enhanced green fluorescent protein (EGFP)-transgenic "N9" induced pluripotent stem cell (iPS cell, iPSC) line from the FVB/N strain-derived mouse embryonic fibroblasts (MEFs). Read More

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http://dx.doi.org/10.1089/cell.2019.0019DOI Listing
October 2019
1.788 Impact Factor

DNA Methylation of the Insulin-Like Growth Factor 2-Imprinted Gene in Trophoblast Cells of Elongated Bovine Embryo: Effects of the Culture.

Cell Reprogram 2019 10;21(5):260-269

Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, Brasília, Brazil.

DNA methylation is an essential epigenetic mark for embryo development and can be susceptible to environment factors such as conditions. The aim of this study was to verify the effect of culture until Day (D) 14 of the development on the embryo size and DNA methylation pattern of the insulin-like growth factor ()-imprinted gene. To achieve this, we produced bovine embryos completely , completely , and until D7 and then up to D14. Read More

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http://dx.doi.org/10.1089/cell.2019.0041DOI Listing
October 2019

Human Menstrual Blood Stem Cell-Derived Granulosa Cells Participate in Ovarian Follicle Formation in a Rat Model of Premature Ovarian Failure .

Cell Reprogram 2019 10;21(5):249-259

Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

We recently reported the application of human menstrual blood stem cells' (HuMenSCs) transplantation as a treatment modality in a rat model of premature ovarian failure (POF). We continued to investigate further in this respect. Female rats were injected intraperitoneally with 36 mg/kg busulfan. Read More

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http://dx.doi.org/10.1089/cell.2019.0020DOI Listing
October 2019

Partially Reprogrammed Induced Pluripotent Stem Cells Using MicroRNA Cluster miR-302s in Guangxi Bama Minipig Fibroblasts.

Cell Reprogram 2019 10 3;21(5):229-237. Epub 2019 Sep 3.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, China.

Pig-induced pluripotent stem cells (piPSCs) have great potential application in regenerative medicine. The miR-302s cluster alone has been shown to reprogram mouse and human somatic cells into induced pluripotent stem cells (iPSCs) without exogenous transcription factors. However, miR-302s alone have not been reported to reprogram cells in large livestock. Read More

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http://dx.doi.org/10.1089/cell.2019.0035DOI Listing
October 2019

Overexpression of MBD3 Improves Reprogramming of Cloned Pig Embryos.

Cell Reprogram 2019 10 8;21(5):221-228. Epub 2019 Aug 8.

National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Methyl-CpG-binding domain protein 3 () is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression level of was demonstrated to be higher in somatic cell nuclear transfer-generated cloned pig embryos than in fertilization-derived porcine embryos. Read More

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http://dx.doi.org/10.1089/cell.2019.0008DOI Listing
October 2019
1 Read

Generation of Induced Pluripotent Cancer Cells from Glioblastoma Multiform Cell Lines.

Cell Reprogram 2019 10 1;21(5):238-248. Epub 2019 Aug 1.

Department Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.

Generation of induced pluripotent stem cells (iPSCs) has been described as a powerful method to dedifferentiate the specialized cells to pluripotency. However, obtaining cancer-specific iPS cells (iPCs) encounters several barriers. The generation of iPCs provides valuable experimental platforms to mimic oncogenesis and offers potentials regarding drug screening. Read More

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http://dx.doi.org/10.1089/cell.2019.0046DOI Listing
October 2019

RepSox Increases Porcine Cloning Efficiency by Improving Pluripotency of Donor Nuclei.

Cell Reprogram 2019 08 16;21(4):181-186. Epub 2019 Jul 16.

1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.

Accumulating evidence suggests that a low pluripotency of donor nuclei might lead to abnormal development of cloned embryos and underlie the inefficiency of mammalian somatic cell nuclear transfer (SCNT). To improve the pluripotency of SCNT embryo, RepSox, a defined small-molecule compound that functions in inhibiting the transforming growth factor β signaling pathway, was used to treat nuclear-transferred porcine oocytes after activation. We found that the developmental ability of porcine SCNT embryos (defined as the blastocyst rate) was significantly increased (13. Read More

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http://dx.doi.org/10.1089/cell.2018.0064DOI Listing

Do the Cytoplast and Nuclear Material of Germinal Vesicle Oocyte Support Developmental Competence Upon Reconstruction with Embryonic/Somatic Nucleus.

Cell Reprogram 2019 08 16;21(4):163-170. Epub 2019 Jul 16.

1Department of Animal & Fish Production, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsaa, Saudi Arabia.

Maturation conditions and oocytes quality have substantial roles on developmental competence of unreconstructed or reconstructed oocytes. Cloning has been reported successfully with low efficiency through embryonic or somatic nuclear transfer into enucleated metaphase II oocytes. It has been suggested that introducing embryonic or somatic nucleus to cytoplast at earlier stage might improve reprogramming of the introduced nucleus. Read More

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http://dx.doi.org/10.1089/cell.2019.0032DOI Listing

Adipose-Derived Stem/Stromal Cells Recapitulate Aging Biomarkers and Show Reduced Stem Cell Plasticity Affecting Their Adipogenic Differentiation Capacity.

Cell Reprogram 2019 08 12;21(4):187-199. Epub 2019 Jul 12.

1Department of Anatomy and Cell Biology, Psychotherapy, Psychosomatic Medicine, Martin Luther University Medical Faculty, Halle, Germany.

Stromal mesenchymal stem cells (MSCs) have the capability to self-renew and can differentiate into multiple cell types of the mesoderm germ layer, but their properties are affected by molecular aging mechanisms. MSCs can be obtained from adipose tissue termed as adipose-derived stem/stromal cells (ASCs) representing a promising tool for studying age-related diseases in detail. ASCs from young (16 weeks) and old (>108 weeks) rabbits were successfully isolated and propagated. Read More

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http://dx.doi.org/10.1089/cell.2019.0010DOI Listing
August 2019
1 Read

Analysis of Human Stem Cell Transcription Factors.

Cell Reprogram 2019 08 12;21(4):171-180. Epub 2019 Jul 12.

Laboratory for Cancer Biology, Departments of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, India.

Transcription factors , , , and are expressed in both human embryonic stem cells (hESCs) and cancer stem cells and they play a crucial role in maintaining characteristics of stemness such as self-renewal and pluripotency. This article evaluates the expression of variants of the main stem cell-specific transcription factors and critically and accurately with specific primers designed for identifying the most important variants that maintain stemness. We have examined four variants of along with a processed pseudogene and seven variants of in human teratocarcinoma cell lines (NTERA2D1, SuSa, GCT-27, and 833KE), hESCs, and ovarian cancer cells by reverse transcriptase-polymerase chain reaction. Read More

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http://dx.doi.org/10.1089/cell.2019.0005DOI Listing
August 2019
1 Read
1.788 Impact Factor

Resveratrol Promotes Osteogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells Through Wnt/Beta-Catenin Signaling Pathway.

Cell Reprogram 2018 12;20(6):371-381

1 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .

Bone marrow mesenchymal stem cells (BMSCs) can transdifferentiate into different types of cells and may serve as a cell source for tissue engineering. Resveratrol has been shown to possess many benefits, including activation of osteogenesis. Furthermore, Wnt/β-catenin signaling has been known to promote osteogenic differentiation in many cells. Read More

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http://dx.doi.org/10.1089/cell.2018.0032DOI Listing
December 2018
6 Reads

Suprachoroidal Adipose Tissue-Derived Mesenchymal Stem Cell Implantation in Patients with Dry-Type Age-Related Macular Degeneration and Stargardt's Macular Dystrophy: 6-Month Follow-Up Results of a Phase 2 Study.

Cell Reprogram 2018 12;20(6):329-336

5 Department of Ophthalmology, Faculty of Medicine, Erciyes University , Kayseri, Turkey .

This prospective clinical case series aimed to investigate the safety and efficacy of suprachoroidal adipose tissue-derived mesenchymal stem cell (ADMSC) implantation in patients with dry-type age-related macular degeneration (AMD) and Stargardt's macular dystrophy (SMD). This study included four patients with advanced-stage dry-type AMD and four patients with SMD who underwent suprachoroidal implantation of ADMSCs. The best-corrected visual acuity (BCVA) in the study was 20/200. Read More

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http://dx.doi.org/10.1089/cell.2018.0045DOI Listing
December 2018
3 Reads

Kidins220/ARMS Expression Confers Proliferation But Independent of Self-Renewal in Mouse Embryonic Stem Cells.

Cell Reprogram 2018 12;20(6):365-370

3 The Institute of Genetics, College of life sciences, Zhejiang University , Hangzhou, China .

Embryonic stem cells (ESCs) are characterized by their ability to self-renew and their potential to differentiate into any cell type. Therefore, identification of novel molecular markers to verify the pluripotent status of mouse ESCs (mESCs) is of great significance. Kinase D interacting substrate of 220 kDa (Kidins220)/ankyrin repeat-rich membrane spanning (ARMS) plays a crucial role in the integration of growth factor receptor pathways during embryonic development. Read More

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http://dx.doi.org/10.1089/cell.2018.0026DOI Listing
December 2018
1 Read

Treatment of Buffalo () Somatic Cell Nuclear Transfer Embryos with MicroRNA-29b Mimic Improves Their Quality, Reduces DNA Methylation, and Changes Gene Expression Without Affecting Their Developmental Competence.

Cell Reprogram 2019 08 14;21(4):210-219. Epub 2019 Jun 14.

Animal Biotechnology Center, ICAR-National Dairy Research Institute, Karnal, India.

microRNA-29b (miR-29b) plays an important role in controlling DNA methylation in cells. We investigated its role during early embryonic development in buffalo embryos produced by somatic cell nuclear transfer (SCNT) and fertilization (IVF). miR-29b expression was highest at the 2-cell stage, decreased ( < 0. Read More

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http://dx.doi.org/10.1089/cell.2019.0007DOI Listing
August 2019
3 Reads
1.788 Impact Factor

Selection of Reference miRNAs for Relative Quantification in Buffalo () Blastocysts Produced by Hand-Made Cloning and Fertilization.

Cell Reprogram 2019 08 14;21(4):200-209. Epub 2019 Jun 14.

1Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, India.

Very low birth rate and a high incidence of abnormalities in offspring born from cloned embryos, which have limited the application of cloning technology on a wide scale, are believed to be because of incomplete or aberrant nuclear reprogramming. MicroRNAs (miRNAs) are involved in regulating a wide range of biological processes including reprogramming and embryonic development. Selection of suitable reference miRNAs is critical for normalization of data for accurate relative quantification of miRNAs by quantitative real-time polymerase chain reaction (qRT-PCR), which is currently the most widely used technique for quantifying miRNAs. Read More

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http://dx.doi.org/10.1089/cell.2019.0022DOI Listing
August 2019
2 Reads
1.788 Impact Factor

Curcumin Promotes Proliferation of Adult Neural Stem Cells and the Birth of Neurons in Alzheimer's Disease Mice via Notch Signaling Pathway.

Cell Reprogram 2019 06;21(3):152-161

3 Department of Obstetrics and Gynecology, Reproductive Medical Center, Daping Hospital & Institute of Surgery Research, Army Medical University (Third Military Medical University), Chongqing, China.

The abnormal deposition of amyloid-β peptide, a major component of senile plaques, has been reported to be the major cause of neuronal cell death and cognitive impairment in Alzheimer's disease (AD). Adult neurogenesis is related to the amelioration of impaired neurons and cognitive impairment. In the research, we investigated the function of curcumin on endogenous neural stem cells (NSCs) and hippocampal neurogenesis in mice. Read More

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http://dx.doi.org/10.1089/cell.2018.0027DOI Listing
June 2019
2 Reads
1.788 Impact Factor

Effect of Silencing on the Autophagy of Osteoblasts.

Authors:
Han Qin Jun Cai

Cell Reprogram 2019 06;21(3):122-128

2 Department of Anesthesia, The Maternal and Child Health Hospital of Lianyungang City, Liangyungang, China.

The objective of the present work was to investigate the effect of silencing on autophagy in osteoblasts, and provide an experimental basis for studying the mechanism of dental eruption. The cells were divided into the following four groups according to their viral number: the NC group, pFU-GW-016PSC53349-1; group KD1, LVpFU-GW-016PSC66471-1; group KD2, LVpFU-GW-016PSC66472-1; and group KD3, LVpFU-GW-016PSC66473-1. The lentiviral vector was infected at MOI = 100 in the ENi. Read More

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http://dx.doi.org/10.1089/cell.2018.0051DOI Listing
June 2019
6 Reads

Preparation of Induced Pluripotent Stem Cells Using Human Peripheral Blood Monocytes.

Cell Reprogram 2018 12;20(6):347-355

1 Department of Respiratory Medicine, Fujita Health University School of Medicine , Toyoake, Japan .

Since induced pluripotent stem (iPS) cells have been established, in recent years, clinical transplantation of cells differentiated from iPS cells derived from human skin fibroblasts is been in progress. On the contrary, monocytes have complete genome information without damage and gene recombination, they are contained in the peripheral blood by ∼3%-8% and differentiate into dendritic cells that are the type of control tower for immune cells. However, generation of monocyte-derived iPS cells has only been successful when special persistent Sendai virus vectors have been used. Read More

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http://dx.doi.org/10.1089/cell.2018.0024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302673PMC
December 2018
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Effects of Cellular Extract on Epigenetic Reprogramming.

Cell Reprogram 2019 06 14;21(3):115-121. Epub 2019 May 14.

1 College of Life Science and Technology, Southwest Minzu University, Chengdu, China.

Functional reprogramming of a differentiated cell toward pluripotent cell may have long-term applications in numerous aspects, especially in regenerative medicine. Evidences accumulating from recent studies suggest that cellular extracts from stem cells or pluripotent cells can induce epigenetic reprogramming and facilitate pluripotency in otherwise highly differentiated cell types. Epigenetic reprogramming using cellular extracts has gained increasing attention and applied to recognize the functional factors, acquire the target cell types, and explain the mechanism of reprogramming. Read More

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https://www.liebertpub.com/doi/10.1089/cell.2018.0074
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http://dx.doi.org/10.1089/cell.2018.0074DOI Listing
June 2019
17 Reads

The Effect of Epigenetic Changes on the Extrusion of the First Polar Body in Pig Oocytes During Maturation.

Cell Reprogram 2019 06 14;21(3):129-140. Epub 2019 May 14.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, P.R. China.

The present study was designed to investigate the comprehensive function of maternal factors of primordial germ cell 7 () and -POU class 5 homeobox 1 (), as well as the epigenetic modification roles on the mitosis for the extrusion of first polar body (PB1) in pig maturated oocytes. First, the common distribution of histone modifications, including H3K4me2, H3K27me3, H3K9me2, and H4K12ac and DNA methylation, were detected at the high level in the nucleus. However, only one part of the chromosome was higher methylated or acetylated when the mitosis happened to extrude the PB1. Read More

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http://dx.doi.org/10.1089/cell.2018.0071DOI Listing
June 2019
2 Reads

Cryptochrome 1 Regulates Osteoblast Differentiation via the AKT Kinase and Extracellular Signal-Regulated Kinase Signaling Pathways.

Cell Reprogram 2019 06 13;21(3):141-151. Epub 2019 Apr 13.

Department of Orthopedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, P.R. China.

The many circadian clock genes build up a network structure that controls physiological processes, such as the sleep cycle, metabolism, and hormone secretion. Cryptochrome 1 (CRY1), as one of the critical circadian proteins, is closely related to bone formation. However, the regulatory function of CRY1 in osteogenic differentiation remains unclear. Read More

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http://dx.doi.org/10.1089/cell.2018.0054DOI Listing
June 2019
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Berberine Accelerates Odontoblast Differentiation by Wnt/β-Catenin Activation.

Cell Reprogram 2019 04;21(2):108-114

4 Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Berberine, a Chinese medical herbal extract, plays a key role in antidiabetic, antiangiogenesis, anti-inflammatory, antimicrobial, anticancer, and antihypercholesterolemic. Our previous studies revealed that berberine exerted odontoprotective effect by increasing odontoblast differentiation. However, the mechanisms involved in the odontoprotective effect of berberine have not been fully explored. Read More

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http://dx.doi.org/10.1089/cell.2018.0060DOI Listing
April 2019
2 Reads