5,540,233 results match your criteria Cellular physiology and biochemistry : international journal of experimental cellular physiology biochemistry and pharmacology[Journal]

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The Neuroprotective Peptide PACAP1-38 Contributes to Horizontal Cell Development in Postnatal Rat Retina.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):770-778

Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs, Hungary.

Purpose: PACAP1-38, a member of the secretin/glucagon superfamily, is expressed in the developing retina with documented neuroprotective effects. However, its function in retinal cell differentiation has yet to be elucidated. Our goals, therefore, were to identify PAC1 expressing cells morphologically, investigate the PACAP1-38 action functionally, and establish PACAP1-38 regulated events developmentally during the first postnatal week in rat retina. Read More

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http://dx.doi.org/10.1167/iovs.18-25719DOI Listing
February 2019

Optimising the biocompatibility of 3D printed photopolymer constructs in vitro and in vivo.

Biomed Mater 2019 Feb 22. Epub 2019 Feb 22.

Department of Surgery, University of Melbourne Faculty of Medicine Dentistry and Health Sciences, Melbourne, Victoria, AUSTRALIA.

3D printing is a rapid and accessible fabrication technology that engenders creative custom design solutions for cell scaffolds, perfusion systems and cell culture systems for tissue engineering. Critical to its success is the biocompatibility of the materials used, which should allow long-term tissue culture without affecting cell viability or inducing an inflammatory response for in vitro and in vivo applications. Polyjet 3D printers offer arguably the highest resolution with the fewest design constraints of any commercially available 3D printing systems. Read More

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http://dx.doi.org/10.1088/1748-605X/ab09c4DOI Listing
February 2019

Epidermal growth factor-nanoparticle conjugates change the activity from anti-apoptotic to pro-apoptotic at membrane rafts.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

WPI Research Initiative for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan; Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. Electronic address:

The proliferation epidermal growth factor (EGF) is known to acquire contradictory apoptotic activities upon conjugation with gold nanoparticles (GNPs) through hitherto unknown mechanisms. Here, we identified an essential role of membrane rafts in the drastic activity switching of EGF-GNPs through the following intracellular signaling. 1) In contrast to the rapid diffusion of activated EGF receptor after the soluble EGF stimulation, the receptor is confined within membrane rafts upon binding to the EGF-GNPs. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.026DOI Listing
February 2019

A transferrin receptor-binding mucoadhesive elastin-like recombinamer: in vitro and in vivo characterization.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

BIOFORGE (Group for Advanced Materials and Nanobiotechnology), CIBER-BBN, University of Valladolid, Paseo de Belén 19, 47011, Spain. Electronic address:

The development of mucoadhesive materials is of great interest and is also a major challenge. Being adsorption sites, mucosae are suitable targets for drug delivery, but as defensive barriers they are complex biological surfaces to interact with, mainly due to their protective mucus layer. As such, first- and second-generation mucoadhesives focused on material-mucus interactions, whereas the third generation of mucoadhesives introduced structural motifs that are able to interact with the cells beneath the mucus layer. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.027DOI Listing
February 2019

Effect of magnesium-degradation products and hypoxia on the angiogenesis of human umbilical vein endothelial cells.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

Institute of Materials Research, Division for Metallic Biomaterials, Helmholtz-Zentrum Geesthacht (HZG), Geesthacht, Germany. Electronic address:

Biodegradable magnesium (Mg) metals have been applied in orthopaedic and stent applications due to their biodegradability, bioabsorbability and adaptability to tissue regeneration. However, further investigations are still needed to understand how angiogenesis will respond to high concentrations of Mg and oxygen content differences, which are vital to vascular remodelling and bone fracture regeneration or tissue healing. Human primary endothelial cells were exposed to various concentrations (2 to 8 mM) of extracellular Mg degradation products under either hypoxia or normoxia. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.018DOI Listing
February 2019

Nanoparticle physicochemical properties determine the activation of intracellular complement.

Nanomedicine 2019 Feb 19. Epub 2019 Feb 19.

Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD. Electronic address:

The complement system plays an essential role in both innate and adaptive immunity. The traditional understanding of this system comes from studies investigating complement proteins produced by the liver and present in plasma to "complement" the immune cell-mediated response to invading pathogens. Recently, it has been reported that immune cells including, but not limited to, T-cells and monocytes, express complement proteins. Read More

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http://dx.doi.org/10.1016/j.nano.2019.02.002DOI Listing
February 2019

Sex biased expression of anti-Mullerian hormone (amh) gene in a live bearing fish, Gambusia holbrooki: Evolutionary implications and potential role in sex differentiation.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 19. Epub 2019 Feb 19.

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania, TAS, Australia; Inland Fisheries and Services (IFS), TAS, Australia.

The amh, a member of transforming growth factor-β (TGF-β) family, is known to play a critical role in vertebrate male sex differentiation, with its paralogue/s evolving to determine sex in few heterogametic (XX/XY) teleosts. However, it remains relatively unexplored in the reproductively unique live bearing teleosts. Therefore, this study comparatively examined the structure and content of G. Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.02.004DOI Listing
February 2019

Heterologous expression of an alternative oxidase from Moniliophthora perniciosa in Saccharomyces cerevisiae: antioxidant function and in vivo platform for the study of new drugs against witches' broom disease.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Laboratório de Biologia Molecular e Biotecnologia Industrial de Microrganismos, Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. Electronic address:

The fungus Moniliophthora perniciosa is the causal agent of witches' broom disease (WBD), one of the most devastating diseases of cacao, the chocolate tree. Many strategies to control WBD have been tested so far, including the use of agrochemicals such as the strobilurins. Strobilurins are fungicides of the QoI family, and they are used in the control of a wide array of fungal diseases in many different crops, including cereals, field crops, fruits, tree nuts, and vegetables. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.006DOI Listing
February 2019

A strikingly-angled spindle mediates nuclear migration during colonization of rice cells infected by Magnaporthe oryzae.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Plant Biology, University of Georgia, Athens, GA 30602, USA. Electronic address:

To cause rice blast disease, Magnaporthe oryzae must properly organize microtubules and position nuclei during colonization of host cells. Live cell confocal imaging of fluorescently-tagged microtubules and nuclei of M. oryzae invasive hyphae reveals that microtubules form a cage-like arrangement around nuclei during interphase and that the mitotic spindle forms and mediates nuclear migration while integrity of the nuclear envelope is lost. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.005DOI Listing
February 2019

Increased CD16 expression on NK cells is indicative of antibody-dependent cell-mediated cytotoxicity in chronic-active antibody-mediated rejection.

Transpl Immunol 2019 Feb 19. Epub 2019 Feb 19.

Department of Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands.

Chronic-active antibody mediated rejection (c-aABMR) contributes significantly to late renal allograft failure. The antibodies directed against donor-derived antigens, e.g. Read More

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http://dx.doi.org/10.1016/j.trim.2019.02.005DOI Listing
February 2019

Prevalence of antibodies to lung self-antigens (Kα1 tubulin and collagen V) and donor specific antibodies to HLA in lung transplant recipients and implications for lung transplant outcomes: Single center experience.

Transpl Immunol 2019 Feb 19. Epub 2019 Feb 19.

Division of Pulmonary and Critical Care Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States. Electronic address:

Purpose: For patients with end stage lung disease, lung transplantation (LT) remains the only definitive treatment option. Long term survival post LT is limited by acute and chronic allograft dysfunction. Antibodies to lung self-antigens Kα1Tubulin and collagen V (autoantibodies) have been implicated in adverse outcomes post LT. Read More

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http://dx.doi.org/10.1016/j.trim.2019.02.006DOI Listing
February 2019

Autologous Transplantation for Male Germ Cell Tumors: Improved Outcomes over 3 decades.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Medical College of Wisconsin, Milwaukee, WI.

Purpose: The curative potential of autologous hematopoietic cell transplantation (autoHCT) for male germ cell tumors (GCT) is well established. Optimal timing and number (single, ST vs. tandem, TT vs. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.015DOI Listing
February 2019

AUTOLOGOUS HEMATOPOIETIC CELL TRANSPLANTATION FOR TREATMENT-REFRACTORY RELAPSING MULTIPLE SCLEROSIS: POSITION STATEMENT FROM THE AMERICAN SOCIETY FOR BLOOD AND MARROW TRANSPLANTATION.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Multiple sclerosis (MS) is a chronic, disabling, immune-mediated, central nervous system demyelinating and degenerative disease. Approved disease modifying therapies may be incompletely effective in some patients with highly active relapsing disease and high risk of disability. Immunoablative or myeloablative therapy followed by autologous hematopoietic cell transplantation (AHCT) has been investigated in retrospective studies, clinical trials, and meta-analyses/systematic reviews as an approach to address this unmet clinical need. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.014DOI Listing
February 2019

Dihydrotanshinone exhibits an anti-inflammatory effect in vitro and in vivo through blocking TLR4 dimerization.

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. Electronic address:

Dihydrotanshinone (DHT), one of the major ingredients of Salvia miltiorrhiza Bunge (Danshen), displays many bioactivities. However, the activity and underlying mechanism of DHT in anti-inflammation have not yet been elucidated. In this study, we investigated the anti-inflammatory activity and molecular mechanism of action of DHT both in vitro and in vivo. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.017DOI Listing
February 2019

The acyl-glucuronide metabolite of ibuprofen has analgesic and anti-inflammatory effects via the TRPA1 channel.

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Florence, Italy. Electronic address:

Ibuprofen is a widely used non-steroidal anti-inflammatory drug (NSAID) that exerts analgesic and anti-inflammatory actions. The transient receptor potential ankyrin 1 (TRPA1) channel, expressed primarily in nociceptors, mediates the action of proalgesic and inflammatory agents. Ibuprofen metabolism yields the reactive compound, ibuprofen-acyl glucuronide, which, like other TRPA1 ligands, covalently interacts with macromolecules. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.019DOI Listing
February 2019

Aromatic monoterpenoid glycosides from rattan stems of Schisandra chinensis and their neuroprotective activities.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin 150040, China. Electronic address:

Six new monoterpenoid glycosides, including thymoquinol 5-O-α-L-arabinopyranosyl-(1 → 6)-β-D-glucopyranoside (1), cuminic acid 7-O-α-D-arabinofuranosyl-(1 → 6)-β-d-glucopyranosyl ester (2), p-cymene 7-O-α-D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (3), p-methylhyd- ratropic acid 9-O-α-D-arabinofuranosyl-(1 → 6)-β-d-glucopyranosyl ester (4), (R)-p-cymene 9-O-α- D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (5), and (R)-p-cymene 9-O-β-D-apiofuranosyl-(1 → 6)-β-D-glucopyranoside (6), together with three known compounds, such as (R)-p-cymene 9-O-β-D-glucopyranoside (7), thymoquinol 5-O-β-D-glucopyranoside (8), and thymoquinol 2-O-β-D-glucopyranoside (9) were isolated from the 70%-EtOH extract of Schisandra chinensis rattan stems. The structures of the new compounds were elucidated by detailed spectroscopic analyses. Meanwhile, the neuroprotective activities of compounds 1-9 were evaluated on the two co-culture models of microglia and neurons cells and astrocytoma and neurons cells. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.012DOI Listing
February 2019

Diverse lignans with anti-inflammatory activity from Urceola rosea.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Seventeen structurally diverse lignans, comprising six new compounds, ecdysanols A (1), B (11), C - E (13-15), and F (17), were isolated from the caulis of Urceola rosea. The structures and absolute configurations of these new compounds were elucidated by means of extensive analysis of mass and NMR data, as well as chiroptical properties. A bioassay in vitro showed that all lignans possessed anti-inflammatory effects by inhibiting the production of TNF-α, NO and/or IL-6 in LPS-stimulated RAW264. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.011DOI Listing
February 2019
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Bis-iridoid and iridoid glycosides: Viral protein R inhibitors from Picrorhiza kurroa collected in Myanmar.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama 930-0194, Japan. Electronic address:

Four new bis-iridoid glycosides, saungmaygaosides A-D (1-4), and six known iridoid glycosides (5-10) were isolated from the n-butanol extract of the stems of Picrorhiza kurroa collected in Myanmar. Their structures were elucidated by extensive spectroscopic techniques. All of the isolates were assayed for anti-Vpr activity, using TREx-HeLa-Vpr cells. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.016DOI Listing
February 2019

Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma.

Gene 2019 Feb 19. Epub 2019 Feb 19.

Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China. Electronic address:

The pathogenesis and the underlying mechanisms of hepatocellular carcinoma (HCC) remain unclear. LncRNA plasmacytoma variant translocation 1 (PVT1) is an oncogene in a variety of cancers. The role of PVT1 in HCC progression is not completely understood. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.036DOI Listing
February 2019

Self-assembly, functionality, and in-vitro properties of quercetin loaded nanoparticles based on shellac-almond gum biological macromolecules.

Int J Biol Macromol 2019 Feb 19. Epub 2019 Feb 19.

Particle and Interfacial Technology Group (PaInT), Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Gent 9000, Belgium.

Quercetin-fortified nanoparticles were prepared from almond gum (AG), a novel biological macromolecule, and Tween 80 (T80) as stabilizers and shellac (SH) as core material using an antisolvent precipitation method. The final nanoparticles were prepared by 0.67% SH, 0. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.02.071DOI Listing
February 2019

Drug repurposing for new, efficient, broad spectrum antivirals.

Virus Res 2019 Feb 19. Epub 2019 Feb 19.

Cell Structure Laboratory, National Center for Biotechnology, National Research Council, CNB-CSIC, Darwin 3, UAM, campus de Cantoblanco, 28049 Madrid, Spain. Electronic address:

Emerging viruses are a major threat to human health. Recent outbreaks have emphasized the urgent need for new antiviral treatments. For several pathogenic viruses, considerable efforts have focused on vaccine development. Read More

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http://dx.doi.org/10.1016/j.virusres.2019.02.011DOI Listing
February 2019

Co-expression of cytokeratin and vimentin in colorectal Cancer highlights a subset of tumor buds and an atypical Cancer-associated stroma.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Institute of Pathology, University of Bern, Murtenstrasse 31, Bern, CH-3008. Electronic address:

Tumor buds in colorectal cancer are hypothesized to undergo a (partial) epithelial-mesenchymal transition (EMT). If so, Cytokeratin (CK) and Vimentin (VIM) co-expression is expected. CK+/VIM+ can also be found in some stromal cells, however their origin remains unclear. Read More

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http://dx.doi.org/10.1016/j.humpath.2019.02.002DOI Listing
February 2019

FAM134B promotes esophageal squamous cell carcinoma in vitro and its correlations with clinicopathologic features.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Australia. Electronic address:

Family with sequence similarity 134, member B (FAM134B) is an autophagy regulator of endoplasmic reticulum first discovered to be involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC). The present study examined the functional behavior of FAM134B in cancer cells and the association of FAM134B expression with clinicopathologic factors in patients with ESCC. Expression at both the mRNA and protein levels was investigated using real-time polymerase chain reaction and immunohistochemistry. Read More

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http://dx.doi.org/10.1016/j.humpath.2018.11.033DOI Listing
February 2019

Tumor PD-L1 expression in malignant pleural and peritoneal mesothelioma by Dako PD-L1 22C3 pharmDx and Dako PD-L1 28-8 pharmDx assays.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pathology, University of Utah, 1950 Circle of Hope, Salt Lake City, UT 84112; ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT, 84108.

Malignant mesothelioma (MM) is an aggressive neoplasm with poor prognosis. The Dako PD-L1 22C3 and 28-8 pharmDx assays are approved by the US Food and Drug Administration (FDA) as companion and complementary diagnostics for the anti-PD-1 drugs pembrolizumab and nivolumab, respectively. Data from multiple clinical trials indicate that immunotherapy has anti-tumor activity in advanced malignant pleural (MPM) and peritoneal mesothelioma (MPeM). Read More

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http://dx.doi.org/10.1016/j.humpath.2019.02.001DOI Listing
February 2019

Reactive cholangiocytes differentiate into proliferative hepatocytes with efficient DNA repair in mice with chronic liver injury.

J Hepatol 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Hepato-gastroenterology, Institut de Recherche Expérimentale et Clinique, Université catholique de Louvain, Brussels, Belgium. Electronic address:

Background And Aim: Chronic liver diseases are characterized by expansion of the small immature cholangiocytes - a mechanism named ductular reaction (DR) - which have the capacity to differentiate in hepatocytes. We investigated the kinetics of DR differentiation into hepatocytes as well as several important features as functional maturity, clonal expansion and resistant to stress of the newly formed hepatocytes in mice with long-term liver damage.

Methods: We tracked cholangiocytes using osteopontin-iCreER and hepatocytes with AAV8-TBG-Cre. Read More

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http://dx.doi.org/10.1016/j.jhep.2019.02.003DOI Listing
February 2019

Multicellular systems biology: quantifying cellular patterning and function in plant organs using network science.

Authors:
George W Bassel

Mol Plant 2019 Feb 19. Epub 2019 Feb 19.

School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK. Electronic address:

Organ function is at least partially shaped and constrained by the organization of their constituent cells. Extensive investigation has revealed mechanisms explaining how these patterns are generated, with less being known about their functional relevance. In this paper, a methodology to discretize and quantitatively analyze cellular patterning is described. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S16742052193005
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http://dx.doi.org/10.1016/j.molp.2019.02.004DOI Listing
February 2019
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Exopolysaccharides synthesized by Bifidobacterium animalis subsp. lactis interact with TLR4 in intestinal epithelial cells.

Anaerobe 2019 Feb 19. Epub 2019 Feb 19.

Microhealth Group, Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias - Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Asturias, Spain. Electronic address:

The toll-like receptors involved in recognition of the exopolysaccharide produced by two isogenic, ropy and non-ropy, Bifidobacterium animalis subsp. lactis strains were investigated. Both strains interact with human embryonic kidney (HEK)-293 cells via TLR2, whereas purified EPSs specifically stimulate TLR4 regardless their molar mass. Read More

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http://dx.doi.org/10.1016/j.anaerobe.2019.02.014DOI Listing
February 2019

Endothelial dysfunction in pregnancy metabolic disorders.

Biochim Biophys Acta Mol Basis Dis 2019 Feb 19. Epub 2019 Feb 19.

Departamento de Ciencias Biologicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370146 Santiago, Chile; Millennium Institute on Immunology and Immunotherapy, 8331150 Santiago, Chile. Electronic address:

In recent years, the vascular endothelium has gained attention as a key player in the initiation and development of pregnancy disorders. Endothelium acts as an endocrine organ that preserves the homeostatic balance by responding to changes in metabolic status. However, in metabolic disorders, endothelial cells adopt a dysfunctional function, losing their normal responsiveness. Read More

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http://dx.doi.org/10.1016/j.bbadis.2019.02.009DOI Listing
February 2019

Influence of traditionally used Nepalese plants on wound healing and immunological properties using primary human cells in vitro.

J Ethnopharmacol 2019 Feb 19. Epub 2019 Feb 19.

Centre for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg, Breisacher Straße 115 B, 79106 Freiburg, Germany. Electronic address:

Ethnopharmacological Relevance: The improvement of wound healing has always been an important issue for both ethnopharmacological and modern medical research. In this study, we used state-of-the-art methods to investigate extracts of plants used traditionally in Nepal for more than 1,000 years to treat inflammatory skin injuries.

Aim Of The Study: We focused on the potential of the plant extracts to ameliorate wound healing and to influence immune modulatory properties. Read More

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http://dx.doi.org/10.1016/j.jep.2019.02.034DOI Listing
February 2019

Dihydrotestosterone activates AP-1 in LNCaP prostate cancer cells.

Int J Biochem Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Medical Biochemistry and Molecular Biology, D-66421 Homburg, Germany.

A cross-talk between androgen/androgen receptor signaling and the AP-1 transcription factor has been proposed. In this study, we asked whether activation of AP-1 modifies androgen-responsive gene transcription, and whether androgens effect AP-1-regulated gene transcription. We show that activation of AP-1 via expression of a constitutively active mutant of mitogen-activated/extracellular signal responsive kinase kinase (MEK) kinase-1 did not increase the activity of the androgen-responsive probasin promoter. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.02.005DOI Listing
February 2019

Anti-cancer effect of Indanone-based thiazolyl hydrazone derivative on colon cancer cell lines.

Int J Biochem Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, 11439, USA. Electronic address:

Colorectal cancer is the third leading cause of cancer related deaths in the United States. Currently, Irinotecan, a topoisomerase I inhibitor, is an approved anti-cancer drug for the treatment of patients with advanced or recurrent colorectal cancer. Considering low response rate and events of high toxicity caused by irinotecan, we evaluated a series of thirteen thiazolyl hydrazone derivatives of 1-indanone for their potential antineoplastic activity and four compounds showed promising anti-cancer activity against most of the tested colon cancer cell lines with IC values ranging from 0. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.02.004DOI Listing
February 2019

Lipid gene nanocarriers for the treatment of skin diseases: current state-of-the-art.

Eur J Pharm Biopharm 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Pharmaceutical Technology and Biopharmacy, CIRM, University of Liege, Belgium.

Nucleic acids carried by non-viral nanovectors have demonstrated high potential as a therapeutic strategy for gene-related diseases. The dermal or transdermal gene delivery allow to target local skin diseases or to reach the blood stream. However, the skin is the first defense barrier of the body and must be overcome to distribute nucleic acids. Read More

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http://dx.doi.org/10.1016/j.ejpb.2019.02.012DOI Listing
February 2019

Protein and surface expression of HCN2 and HCN4 subunits in mesocorticolimbic areas after cocaine sensitization.

Neurochem Int 2019 Feb 19. Epub 2019 Feb 19.

Physiology Department, University of Puerto Rico, School of Medicine, Medical Sciences Campus, San Juan, 00936-5067, Puerto Rico. Electronic address:

The I is a mixed depolarizing current present in neurons which, upon activation by hyperpolarization, modulates neuronal excitability in the mesocorticolimbic (MCL) system, an area which regulates emotions such as pleasure, reward, and motivation. Its biophysical properties are determined by HCN protein expression profiles, specifically HCN subunits 1-4. Previously, we reported that cocaine-induced behavioral sensitization increases HCN2 protein expression in all MCL areas with the Ventral Tegmental Area (VTA) showing the most significant increase. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.02.004DOI Listing
February 2019

Propagermanium, a CCR2 inhibitor, attenuates cerebral ischemia/reperfusion injury through inhibiting inflammatory response induced by microglia.

Neurochem Int 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Natural Medicines, School of Basic Medical Sciences and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China. Electronic address:

CCR2 could recruit immune cells migrating into brain after ischemic stroke. It is unclear whether and why Propagermanium (PG, a CCR2 inhibitor) is able to protect against ischemic injury. Middle cerebral artery occlusion (MCAO) and reperfusion injury in C57BL/6 J male mice were performed in vivo to mimic ischemic stroke. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.02.010DOI Listing
February 2019

Cell pelotons: a model of early evolutionary cell sorting, with application to slime mold D. discoideum.

Authors:
Hugh Trenchard

J Theor Biol 2019 Feb 19. Epub 2019 Feb 19.

Independent researcher, 805 647 Michigan Street, Victoria BC Canada V8V 1S9, 250-472-0718. Electronic address:

A theoretical model is presented for early evolutionary cell sorting within cellular aggregates. The model involves an energy-saving mechanism and principles of collective self-organization analogous to those observed in bicycle pelotons (groups of cyclists). The theoretical framework is applied to slime-mold slugs (Dictyostelium discoideum) and incorporated into a computer simulation which demonstrates principally the sorting of cells between the anterior and posterior slug regions. Read More

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http://dx.doi.org/10.1016/j.jtbi.2019.02.011DOI Listing
February 2019

Cytoplasmic OH scavenger TA293 attenuates cellular senescence and fibrosis by activating macrophages through oxidized phospholipids/TLR4.

Life Sci 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Physiological Chemistry, Faculty of Pharmacy, Takasaki University of Health and Welfare, 60 Nakaorui-machi, Takasaki, Gunma 370-0033, Japan.

Aims: Elucidation of the biological roles of the mitochondrial and cytoplasmic hydroxyl radical (cyto OH) is hampered by the absence of site-specific OH scavengers. Earlier findings using cyto OH scavenger, TA293, indicated that cyto OH causes cellular senescence, and senescence-associated secretory phenotype (SASP) factors secreted from cells cause macrophage infiltration, inflammation, and apoptosis. However, we found that macrophage infiltration occurs before senescent cells appear. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.02.038DOI Listing
February 2019

Baicalin exerts antidepressant effects through Akt/FOXG1 pathway promoting neuronal differentiation and survival.

Life Sci 2019 Feb 19. Epub 2019 Feb 19.

Qinba Traditional Chinese Medicine Resources Research and Development Center, AnKang University, AnKang 725000, PR China; Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, 639, Longmian Road, Nanjing 211198, China. Electronic address:

Background And Aims: Impaired neurogenesis in hippocampus may contribute to a variety of neurological diseases, such as Alzheimer's disease and depression. Baicalin (BA), which is mainly isolated from the root Scutellaria baicalensis Georgi (traditional Chinese herb), which was reported to facilitate neurogenesis, but how to play the role and the underlying molecular mechanisms are still unknown.

Main Methods: In this study, we adopted the chronic unpredictable mild stress (CUMS)-induced mouse model of depression, and then explore antidepressant-like effects and possible molecular mechanisms. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.02.033DOI Listing
February 2019

Reductively cleavable polymer-drug conjugates based on dendritic polyglycerol sulfate and monomethyl auristatin E as anticancer drugs.

J Control Release 2019 Feb 19. Epub 2019 Feb 19.

Institute for Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, Berlin 14195, Germany. Electronic address:

Stimuli-responsive polymer-drug conjugates (PDCs) provide promising approaches in anticancer treatment. Here, we report the synthesis and biological evaluation of PDCs made of the highly potent antimitotic agent monomethyl auristatin E conjugated to dendritic polyglycerol and dendritic polyglycerol sulfate via a reductively cleavable, self-immolative disulfide linker. Cell viability assays with the human cancer cell lines A549 (lung carcinoma) and HeLa (cervix carcinoma) revealed that the drug's cytotoxicity was reduced by conjugation to the polymers, with the sulfated conjugates being more effective than the non-sulfated ones. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.01.035DOI Listing
February 2019

Heparan sulfate in chronic kidney diseases: Exploring the role of 3-O-sulfation.

Biochim Biophys Acta Gen Subj 2019 Feb 19. Epub 2019 Feb 19.

Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, NE2 4HH, UK. Electronic address:

One of the main feature of chronic kidney disease is the development of renal fibrosis. Heparan Sulfate (HS) is involved in disease development by modifying the function of growth factors and cytokines and creating chemokine gradients. In this context, we aimed to understand the function of HS sulfation in renal fibrosis. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.009DOI Listing
February 2019

Iron-oxide nanoparticles target intracellular HSP90 to induce tumor radio-sensitization.

Biochim Biophys Acta Gen Subj 2019 Feb 19. Epub 2019 Feb 19.

Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India; Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400085, India. Electronic address:

Background: Nanoparticle-based therapies have emerged as a promising approach to overcome limitations of conventional chemotherapy. Present study investigates the potential of oleic acid-functionalized iron-oxide nanoparticles (MN-OA) to enhance the radiation response of fibrosarcoma tumor and elucidates its underlying mechanism.

Methods: Various cellular and molecular assays (e. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.010DOI Listing
February 2019

MicroRNA-9 affects isolated ovarian granulosa cells proliferation and apoptosis via targeting vitamin D receptor.

Mol Cell Endocrinol 2019 Feb 19. Epub 2019 Feb 19.

Obstetrical Department, Henan Provincial People's Hospital, Zhengzhou, China.

MicroRNAs (miRNAs or miRs)-9 expression was reported to be upregulated in the follicular fluid of patients with polycystic ovary syndrome (PCOS). However, whether miR-9 affects ovarian dysfunction of PCOS and the related mechanisms are still unclear. Here we detected miR-9 and vitamin D receptor (VDR) expression in women with PCOS and controls, and investigated whether miR-9 affects ovarian granulosa cells (GCs) proliferation and apoptosis by targeting VDR. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.012DOI Listing
February 2019

Genetic strain and sex differences in a hyperoxia-induced mouse model of varying severity of bronchopulmonary dysplasia.

Am J Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pediatrics, Drexel University College of Medicine, Philadelphia, PA; St. Christopher's Hospital for Children, Philadelphia, PA. Electronic address:

Bronchopulmonary dysplasia (BPD) is a disease prevalent in preterm babies with a need for supplemental oxygen resulting in impaired lung development and dysregulated vascularization. Epidemiological studies have shown that males are more prone to BPD and have a delayed recovery compared to females for reasons unknown. Here, we tried to recapitulate mild, moderate, and severe BPD, using two different strains of mice: CD1 (outbred) and C57BL/6 (inbred), in males and females. Read More

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http://dx.doi.org/10.1016/j.ajpath.2019.01.014DOI Listing
February 2019

Intrinsic Bone Defects in Cystinotic Mice.

Am J Pathol 2019 Feb 19. Epub 2019 Feb 19.

Bone Physiopathology Group, Multifactorial Disease and Complex Phenotype Research Area, Bambino Gesù Children's Hospital, Rome, Italy.

Cystinosis is a rare lysosomal storage disorder caused by loss of function mutations of the CTNS gene, encoding cystinosin, a symporter that mediates cystine efflux from lysosomes. About 95% of patients with cystinosis display renal Fanconi syndrome, short stature, osteopenia, and rickets . In this study, we investigated whether the absence of cystinosin primarily affects bone remodeling activity, apart from the influences of the Fanconi syndrome on bone mineral metabolism. Read More

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http://dx.doi.org/10.1016/j.ajpath.2019.01.015DOI Listing
February 2019

Mechanical influences on Cardiovascular Differentiation and Disease Modeling.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037. Electronic address:

Tissues are continuously exposed to forces in vivo, whether from fluid pressure in an artery from our blood or compressive forces on joints from our body weight. The forces that cells are exposed to arise almost immediately after conception; it is therefore important to understand how forces shape stem cell differentiation into lineage committed cells, how they help organize cells into tissues, and how forces can cause or exacerbate disease. No tissue is exempt, but cardiovascular tissues in particular are exposed to these forces. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.019DOI Listing
February 2019

Interleukin-6 released from differentiating human beige adipocytes improves browning.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, University of Debrecen, Faculty of Medicine, Debrecen, Hungary; MTA-DE Stem Cells, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary. Electronic address:

Brown and beige adipocytes contribute significantly to the regulation of whole body energy expenditure and systemic metabolic homeostasis not exclusively by thermogenesis through mitochondrial uncoupling. Several studies have provided evidence in rodents that brown and beige adipocytes produce a set of adipokines ("batokines") which regulate local tissue homeostasis and have beneficial effects on physiological functions of the entire body. We observed elevated secretion of Interleukin (IL) -6, IL-8 and monocyte chemoattractant protein (MCP) -1, but not tumor necrosis factor alpha (TNFα) or IL-1β pro-inflammatory cytokines, by ex vivo differentiating human beige adipocytes (induced by either PPARγ agonist or irisin) compared to white. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.015DOI Listing
February 2019

On the spatiotemporal regulation of cell tensional state.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Univ. Grenoble Alpes, CNRS, LIPhy, F-38000 Grenoble, France.

Since the emergence of mechanobiology, mechanical signals have been shown to influence almost every process in biology. Cells transduce mechanical signals into biochemical signaling pathways, adjust their behavior and/or phenotype before transmitting these signals to neighboring cells. Mechanical signals thus appear as information, which can be "written" by cells in the surrounding extracellular matrix, "transmitted" through it and "read" by other cells. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.018DOI Listing
February 2019

Loss of ALDH1L1 folate enzyme confers a selective metabolic advantage for tumor progression.

Chem Biol Interact 2019 Feb 19. Epub 2019 Feb 19.

Department of Nutrition, UNC-Chapel Hill, UNC Nutrition Research Institute, Kannapolis, NC, 28081, United States.

ALDH1L1 (cytosolic 10-formyltetrahydrofolate dehydrogenase) is the enzyme in folate metabolism commonly downregulated in human cancers. One of the mechanisms of the enzyme downregulation is methylation of the promoter of the ALDH1L1 gene. Recent studies underscored ALDH1L1 as a candidate tumor suppressor and potential marker of aggressive cancers. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.02.013DOI Listing
February 2019

Hepatic metabolic adaptation in a murine model of glutathione deficiency.

Chem Biol Interact 2019 Feb 19. Epub 2019 Feb 19.

Department of Environmental Health Sciences, Yale School of Public Health, New Haven, CT, 06521, USA. Electronic address:

Glutathione (GSH), the most abundant cellular non-protein thiol, plays a pivotal role in hepatic defense mechanisms against oxidative damage. Despite a strong association between disrupted GSH homeostasis and liver diseases of various etiologies, it was shown that GSH-deficient glutamate-cysteine ligase modifier subunit (Gclm)-null mice are protected against fatty liver development induced by a variety of dietary and environmental insults. The biochemical mechanisms underpinning this protective phenotype have not been clearly defined. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.02.015DOI Listing
February 2019

Quality by Design Driven Process Development of Cell Culture in Bioreactor for the Production of Foot-and-Mouth Veterinary Vaccine.

J Pharm Sci 2019 Feb 19. Epub 2019 Feb 19.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

Quality by design (QbD) principle has been established as a guideline to emphasize the understanding of the relationship of product quality with process control. Vaccine product have characteristics of security and high efficiency, but it also has features like complexity and rigorous regulatory for production. This case study describes an example of QbD driven process development for manufacturing a veterinary vaccine produced with baby hamster kidney-21 (BHK-21) cells. Read More

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http://dx.doi.org/10.1016/j.xphs.2019.02.004DOI Listing
February 2019

Vive ut Numquam Moriturus: Tweaking Translational Control to Regulate Longevity.

Mol Cell 2019 Feb;73(4):643-644

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA. Electronic address:

Molecular mechanisms regulating aging at the post-transcriptional level are not clear. In this issue of Molecular Cell,D'Amico et al. (2019) demonstrate that the translational inhibition of mitochondrial fission factor (MFF) regulates cellular homeostasis and aging. Read More

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http://dx.doi.org/10.1016/j.molcel.2019.02.006DOI Listing
February 2019
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