5,875,617 results match your criteria Cellular physiology and biochemistry : international journal of experimental cellular physiology biochemistry and pharmacology[Journal]

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Picolinic acid, a catabolite of tryptophan, has an anabolic effect on bone in vivo.

J Bone Miner Res 2020 Jul 6. Epub 2020 Jul 6.

Neuroinflammation Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, New South Wales, Australia.

Fractures due to osteoporosis have a severe impact on our older population. Reports of side effects with commonly prescribed osteoporosis drugs have led to the investigation of new and safer treatments with novel mechanisms of action. Picolinic acid (PIC), a catabolite of tryptophan, induces in vitro osteogenic differentiation of mesenchymal stem cells. Read More

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http://dx.doi.org/10.1002/jbmr.4125DOI Listing

Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration.

Xenotransplantation 2020 Jul 6:e12625. Epub 2020 Jul 6.

Division of Orthodontics, College of Dentistry, The Ohio State University, Columbus, OH, USA.

Background: It is commonly accepted that xenogeneic stem cell transplantation for tissue engineering is faced with host immune rejection. Using a rat critical-size mandibular defect model, this study examined whether the immune rejection can be evaded by diminishing T-cell immunity.

Methods: To examine donor cell survival and host immune reaction, pig bone marrow-derived mesenchymal stem cells (BM-MSCs) were labeled with CM-DiI, loaded onto gelatin sponge (5 × 10 cells/scaffold), and transplanted into 5-mm mandibular defects of immunocompetent and T cell-deficient athymic rats. Read More

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http://dx.doi.org/10.1111/xen.12625DOI Listing

Oncogenic mutation in RAS-RAF axis leads to increased expression of GREB1 resulting in tumor proliferation in CRC.

Cancer Sci 2020 Jul 6. Epub 2020 Jul 6.

Department of Gastroenterological and Transplant Surgery, Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.

BRAF mutation accounts for up to 90 % of all BRAF mutations in human colorectal cancer (CRC), and constitutively activates the MEK-MAPK pathway. It is recognized that neutralizing monoclonal antibodies for epidermal growth factor receptor alone are not effective for CRC with BRAF mutation. Therefore, there are increasing interests in identification of the possible therapeutic targets in downstream of BRAF mutation in CRCs. Read More

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http://dx.doi.org/10.1111/cas.14558DOI Listing

Inflammatory Cell Infiltration of Adrenals in COVID-19.

Horm Metab Res 2020 Jul 6. Epub 2020 Jul 6.

Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was originated in November-December 2019 in Wuhan, China, and has rapidly spread around the world causing severe health and socioeconomical damage to the entire civilization. The key feature of coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is upper respiratory tract infection, which may be complicated by bilateral pneumonia. Angiotensin converting enzyme 2 (ACE2) has been identified as a key host factor, required for virus entry into cells. Read More

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http://dx.doi.org/10.1055/a-1191-8094DOI Listing

The Role of Streptococcus pneumoniae in Community-Acquired Pneumonia.

Semin Respir Crit Care Med 2020 Aug 13;41(4):455-469. Epub 2020 Jun 13.

Department of Immunology and Institute of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

With the notable exceptions of the United States and Canada in particular, the global burden of disease in adults due to invasive infection with the dangerous respiratory, bacterial pathogen, (pneumococcus) remains. This situation prevails despite the major successes of inclusion of polysaccharide conjugate vaccines (PCVs) in many national childhood immunization programs and associated herd protection in adults, as well as the availability of effective antimicrobial agents. Accurate assessment of the geographic variations in the prevalence of invasive pneumococcal disease (IPD) has, however, been somewhat impeded by the limitations imposed on the acquisition of reliable epidemiological data due to reliance on often insensitive, laboratory-based, pathogen identification procedures. Read More

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http://dx.doi.org/10.1055/s-0040-1702193DOI Listing

New Horizons: Novel Adrenal Regenerative Therapies.

J Clin Endocrinol Metab 2020 Jul 6. Epub 2020 Jul 6.

Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Adrenal insufficiency requires lifelong corticoid replacement therapies. However, current therapies are not able to replace the physiological circadian pattern of the adrenal cortex and are associated with many metabolic, vascular, neuroendocrine and mental perturbations. Therefore, regenerative and more curative strategies would be desirable. Read More

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http://dx.doi.org/10.1210/clinem/dgaa438DOI Listing

Analysis of Innate and Adaptive Immunological Characteristics in Patients with IgG4-Related Disease.

Int Arch Allergy Immunol 2020 Jul 6:1-6. Epub 2020 Jul 6.

Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea,

Background: Immunoglobulin G4-related disease (IgG4-RD) is a systemic immunological disorder characterized by fibro-inflammatory conditions; however, the pathobiology of IgG4-RD has not been fully identified.

Objective: This study aimed to analyze systemic differences of innate and adaptive immune cells from healthy controls and patients with IgG4-RD.

Methods: Healthy controls (n = 9) and IgG4-RD patients (n = 7) were recruited with informed consent. Read More

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http://dx.doi.org/10.1159/000508699DOI Listing

Reliable inkjet printing of chondrocytes and MSCs using reservoir agitation.

Biofabrication 2020 Jul 6. Epub 2020 Jul 6.

School of Engineering, Newcastle University, Newcastle upon Tyne, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND.

Drop-on-demand (DoD) inkjet printing has been explored for a range of applications, including those to selectively deposit cellular material, due to the high accuracy and scalability of such systems when compared with alternative bioprinting techniques. Despite this, there remains considerable limitations when handling cell suspensions due to the agglomeration and sedimentation of cells during printing, leading to a deterioration in jetting performance. The objective of this work was to design and assess the effectiveness of a custom agitation system to maintain cellular dispersion within the ink reservoir during printing. Read More

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http://dx.doi.org/10.1088/1758-5090/aba2f8DOI Listing

Osteogenic differentiation of bone marrow mesenchymal stem cells on chitosan/gelatin scaffolds: Gene expression profile and mechanical analysis.

Biomed Mater 2020 Jul 6. Epub 2020 Jul 6.

Haemopoiesis Research Laboratory, University of Crete School of Medicine, Heraklion, GREECE.

In the present study we explore the extracellular matrix produced by human bone marrow mesenchymal stem/stromal cells (BM-MSCs) induced to undergo osteogenic differentiation within porous chitosan/gelatin (CS:Gel) scaffolds by investigating their multiple gene expression profile and mechanical behavior. Initially, the efficiency of the BM-MSCs osteogenic differentiation within the constructs was confirmed by the significant rise in the expression of the osteogenesis associated genes DLX5, RUNX2, ALP and OSC. In line with these findings, OSC and Col1A1 protein expression was also detected in BM-MSCs on the CS:Gel scaffolds at day 14 of osteogenic differentiation. Read More

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http://dx.doi.org/10.1088/1748-605X/aba325DOI Listing

Does the Inion OTPSabsorbable plating system induce higher foreign-body reaction than titanium implants? An experimental randomized comparative study in rabbits.

Biomed Mater 2020 Jul 6. Epub 2020 Jul 6.

1st Orthopaedic Department, Aristoteleio Panepistemio Thessalonikes Schole Epistemon Ygeias, Thessaloniki, Thessaloniki, GREECE.

Hardware removal after complete fracture healing is a laborious and traumatic procedure that bears the risks of refracture, infection, and soft tissue damage. Absorbable materials for fracture fixation have the advantage of eliminating the need for removal, however, they may stimulate a considerable inflammatory response. For that reason, we compared in a prospective randomized experimental animal model study the incidence of foreign-body reactions between Inion OTPSTM absorbable plate implants, consisting of carbon trimethylene, L-polylactic acid, and D, L-polylactic acid, and conventional titanium plates. Read More

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http://dx.doi.org/10.1088/1748-605X/aba326DOI Listing

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition.

Aging (Albany NY) 2020 Jul 6;12. Epub 2020 Jul 6.

Department of Cardiovascular Surgery, Provincial Clinical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, P.R. China.

Long non-coding RNAs (lncRNAs) have been largely reported to contribute to the development and progression of abdominal aortic aneurysm (AAA), a common vascular degenerative disease. The present study was set out with the aim to investigate the possible role of lncRNA Sox2ot in the development of AAA. In this study, we found that lncRNA Sox2ot and early growth response factor-1 (Egr1) were highly expressed, while microRNA (miR)-145 was poorly expressed in Ang II-induced AAA mice and oxidative stress-provoked vascular smooth muscle cell (VSMC) model. Read More

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http://dx.doi.org/10.18632/aging.103077DOI Listing

Drosophila PTPMT1 Has a Function in Tracheal Air Filling.

iScience 2020 Jun 20;23(7):101285. Epub 2020 Jun 20.

Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address:

The fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal air filling. Read More

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http://dx.doi.org/10.1016/j.isci.2020.101285DOI Listing

Damage-associated molecular patterns in tumor radiotherapy.

Int Immunopharmacol 2020 Jul 3;86:106761. Epub 2020 Jul 3.

Radiology and Nuclear Medicine Department, School of Paramedical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address:

Radiotherapy is one of the most common modalities for the treatment of cancer. One of the most promising effects of radiotherapy is immunologic cell death and the release of danger alarms, which are known as damage-associated molecular patterns (DAMPs). DAMPs are able to trigger cancer cells and other cells within tumor microenvironment (TME), either for suppression or promotion of tumor growth. Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106761DOI Listing

Cu-selective NIR fluorescence sensor based on heptamethine cyanine in aqueous media and its application.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Jun 19;240:118606. Epub 2020 Jun 19.

Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand. Electronic address:

A near-infrared (NIR) colorimetric fluorescence sensor, Cy7C3, based on heptamethine cyanine dye was synthesized for determining the presence of Cu ions. The sensor showed highly sensitive fluorescence quenching toward Cu ions in acetonitrile/buffer solution at physiological pH with long emission wavelength of 718 nm. Cy7C3 also provided an excellent selectivity to Cu ions over other competing metal ions, with a low detection limit of 9 ppb, which was lower than the maximum concentration of Cu ions in drinking water of U. Read More

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http://dx.doi.org/10.1016/j.saa.2020.118606DOI Listing

A FRET-based ratiometric fluorescent probe for highly selective detection of hydrogen polysulfides based on a coumarin-rhodol derivative.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Jun 29;241:118650. Epub 2020 Jun 29.

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.

In modern biology, hydrogen polysulfides (HS, n > 1) are members of reactive sulfur species (RSS), with anti-oxidation, cell protection and redox signals in tissues and organs. Therefore, it is crucial to develop a method to monitor the changes of HS level in organisms. We designed and synthesized a ratiometric fluorescent probe for highly selective detection of HS based on the fluorescence resonance energy transfer (FRET) process. Read More

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http://dx.doi.org/10.1016/j.saa.2020.118650DOI Listing

Hematopoietic stem-cell senescence and myocardial repair - Coronary artery disease genotype/phenotype analysis of post-MI myocardial regeneration response induced by CABG/CD133+ bone marrow hematopoietic stem cell treatment in RCT PERFECT Phase 3.

EBioMedicine 2020 Jul 3;57:102862. Epub 2020 Jul 3.

Reference and Translation Center for Cardiac Stem Cell Therapy, Department Life, Light and Matter and Department of cardiac surgery, University Medicine Rostock, Schillingallee 35, 18055 Rostock, Germany. Electronic address:

Background: Bone marrow stem cell clonal dysfunction by somatic mutation is suspected to affect post-infarction myocardial regeneration after coronary bypass surgery (CABG).

Methods: Transcriptome and variant expression analysis was studied in the phase 3 PERFECT trial post myocardial infarction CABG and CD133 bone marrow derived hematopoetic stem cells showing difference in left ventricular ejection fraction (∆LVEF) myocardial regeneration Responders (n=14; ∆LVEF +16% day 180/0) and Non-responders (n=9; ∆LVEF -1.1% day 180/0). Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102862DOI Listing

Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma.

EBioMedicine 2020 Jul 3;57:102846. Epub 2020 Jul 3.

Department of Internal Medicine, National Taiwan University Hospital, No. 7, Zhongshan S Rd, Zhongzheng District, Taipei 10002, Taiwan; Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No. 1, Jen Ai Rd, Section 1, Zhongzheng District, Taipei 10051, Taiwan. Electronic address:

Background: Aberrant fucosylation plays a critical role in lung cancer progression. Nevertheless, the key fucosyltransferase with prognostic significance in lung cancer patients, the enzyme's intracellular targets, and complex molecular mechanisms underlying lung cancer metastasis remain incompletely understood.

Methods: We performed a large-scale transcriptome-clinical correlation to identify major fucosyltransferases with significant prognostic values. Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102846DOI Listing

Identification of unique and shared mitochondrial DNA mutations in neurodegeneration and cancer by single-cell mitochondrial DNA structural variation sequencing (MitoSV-seq).

EBioMedicine 2020 Jul 3;57:102868. Epub 2020 Jul 3.

Neuroinflammation Unit, Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen, Denmark. Electronic address:

Background: Point mutations and structural variations (SVs) in mitochondrial DNA (mtDNA) contribute to many neurodegenerative diseases. Technical limitations and heteroplasmy, however, have impeded their identification, preventing these changes from being examined in neurons in healthy and disease states.

Methods: We have developed a high-resolution technique-Mitochondrial DNA Structural Variation Sequencing (MitoSV-seq)-that identifies all types of mtDNA SVs and single-nucleotide variations (SNVs) in single neurons and novel variations that have been undetectable with conventional techniques. Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102868DOI Listing

Single cell genomics and developmental biology: moving beyond the generation of cell type catalogues.

Curr Opin Genet Dev 2020 Jul 3;64:66-71. Epub 2020 Jul 3.

Department of Haematology, University of Cambridge, Cambridge, UK; Wellcome-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. Electronic address:

Major developmental processes such as gastrulation and early embryogenesis rely on a complex network of cell-cell interactions, chromatin remodeling, and transcriptional regulators. This makes it challenging to study early development when using bulk populations of cells. Recent advances in single-cell technologies have allowed researchers to better understand the interactions between different molecular modalities and the heterogeneities within classically defined cell types. Read More

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http://dx.doi.org/10.1016/j.gde.2020.05.033DOI Listing

Non-nuke HIV-1 inhibitor shuttled by mesoporous silica nanoparticles effectively slows down HIV-1 replication in infected human cells.

Colloids Surf B Biointerfaces 2020 Jun 26;194:111227. Epub 2020 Jun 26.

Department of Virology, ICMR-National AIDS Research Institute, Pune, 411 026, Maharashtra, India; Savitribai Phule Pune University, Ganeshkhind, Pune 411 007, India. Electronic address:

The objectives of this study were to reduce the cytotoxic effect of nevirapine (NVP) and to enhance its anti-HIV efficacy through mesoporous silica nanoparticles (MSNPs) mediated delivery. MSNPs were synthesized and characterized by various techniques. Confocal microscopy and flow cytometry results exhibited efficient uptake of FITC-conjugated MSNPs in TZM-bl cells. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2020.111227DOI Listing

Treatment of MOG-IgG-associated disorder with rituximab: An international study of 121 patients.

Mult Scler Relat Disord 2020 Jun 2;44:102251. Epub 2020 Jun 2.

Department of Neurology, The Walton Centre NHS Foundation Trust, Liverpool, United Kingdom; Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom; Department of Neurology, The Cleveland Clinic Abu Dhabi, United Arab Emirates. Electronic address:

Objective: To assess the effect of anti-CD20 B-cell depletion with rituximab (RTX) on relapse rates in myelin oligodendrocyte glycoprotein antibody-associated disorder (MOGAD).

Methods: Retrospective review of RTX-treated MOGAD patients from 29 centres in 13 countries. The primary outcome measure was change in relapse rate after starting rituximab (Poisson regression model). Read More

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http://dx.doi.org/10.1016/j.msard.2020.102251DOI Listing

The evolution of the human brain and disease susceptibility.

Curr Opin Genet Dev 2020 Jul 3;65:91-97. Epub 2020 Jul 3.

Department of Neuroscience, Yale School of Medicine, New Haven, CT 06510, USA; Yale Child Study Center, New Haven, CT 06510, USA; Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA; Departments of Psychiatry and Comparative Medicine, Kavli Institute for Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale School of Medicine, New Haven, CT 06510, USA. Electronic address:

Evolutionary perspective is critical for understanding human biology, human medicine, and the traits that make human beings unique. One of the crucial characteristics that sets humans apart from other extant species is our cognitive ability, which allows for complex processes including symbolic thought, theory of mind, and syntactical-grammatical language, and is thought to arise from the expansion and specialization of the human nervous system. It has been hypothesized that the same evolutionary changes that allowed us to develop these valuable skills made humans susceptible to neurodevelopmental and neurodegenerative disease. Read More

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http://dx.doi.org/10.1016/j.gde.2020.05.004DOI Listing

Nutritional deficiency and placenta calcification underlie constitutive, selective embryo loss in pregnant South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha).

Theriogenology 2020 Jun 7;155:77-87. Epub 2020 Jun 7.

Centro de Estudios Biomédicos Básicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimónides, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address:

Plains vizcacha females are able to ovulate up to 800 oocytes per estrus cycle. However, just 10-12 embryos are implanted and only two of them, those located nearest the cervix, are gestated to term. Between 26 and 70 days post-coitum, a constitutive resorption occurs from the embryos located proximal to the ovary, extending progressively toward those distally implanted. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2020.06.003DOI Listing

Engineering and modeling of multicellular morphologies and patterns.

Curr Opin Genet Dev 2020 Jul 3;63:95-102. Epub 2020 Jul 3.

Department of Molecular and Cellular Biology, University of Arizona, Tucson, USA. Electronic address:

Synthetic multicellular (MC) systems have the capacity to increase our understanding of biofilms and higher organisms, and to serve as engineering platforms for developing complex products in the areas of medicine, biosynthesis and smart materials. Here we provide an interdisciplinary perspective and review on emerging approaches to engineer and model MC systems. We lay out definitions for key terms in the field and identify toolboxes of standardized parts which can be combined into various MC algorithms to achieve specific outcomes. Read More

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http://dx.doi.org/10.1016/j.gde.2020.05.039DOI Listing

Gm15575 functions as a ceRNA to up-regulate CCL7 expression through sponging miR-686 in Th17 cells.

Mol Immunol 2020 Jul 3;125:32-42. Epub 2020 Jul 3.

Jiangsu Key Laboratory of Druggability of Biopharmaceuticals and State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Compelling evidence has demonstrated that Th17 cells play an essential role in the pathogenesis of multiple sclerosis (MS). Long noncoding RNAs (lncRNAs) have been confirmed as vital regulators of immune cell differentiation and other functions. However, whether and how lncRNAs influence Th17 cell differentiation and functional behaviors remain largely unclear. Read More

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http://dx.doi.org/10.1016/j.molimm.2020.06.027DOI Listing

Degradation and deactivation of a plasmid-encoded extracellular antibiotic resistance gene during separate and combined exposures to UV and radicals.

Water Res 2020 May 16;182:115921. Epub 2020 May 16.

School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, 61005, Republic of Korea. Electronic address:

This study investigated the degradation and deactivation of an extracellular ampicillin resistance gene (amp) encoded in plasmid pUC19 during exposure to UV, OH (generated by UV/HO), and combined exposure to UV and OH (and/or SO) using UV/HO and UV/SO. The degradation rates of amp measured by quantitative polymerase chain reaction increased with increasing target amplicon length (192-851 bps). The rate constants for the degradation of pUC19 (2686 bps) were calculated as 0. Read More

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http://dx.doi.org/10.1016/j.watres.2020.115921DOI Listing

Interleukin-18 levels and mouse Leydig cell apoptosis during lipopolysaccharide-induced acute inflammatory conditions.

J Reprod Immunol 2020 Jun 20;141:103167. Epub 2020 Jun 20.

Department of Emergency, Disaster and Critical Care Medicine, Hyogo College of Medicine, 1-1, Mukogawa-cho, Nishinomiya, 663-8501 Hyogo, Japan.

Interleukin (IL)-18 is an inflammasome-mediated cytokine produced by germ cells, Leydig cells, and resident macrophages that is indispensable in the maintenance of homeostasis in the testis. We previously demonstrated that endogenous IL-18 induces testicular germ cell apoptosis during acute inflammation when plasma IL-18 levels are very high. However, the impact of acute inflammation and IL-18 on Leydig cells remained unclear. Read More

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http://dx.doi.org/10.1016/j.jri.2020.103167DOI Listing

Zebrafish brain RNA sequencing reveals that cell adhesion molecules are critical in brain aging.

Neurobiol Aging 2020 May 12;94:164-175. Epub 2020 May 12.

Stem Cell Research and Application Center, Hacettepe University, Ankara, Turkey. Electronic address:

Brain aging is a complex process, which involves multiple pathways including various components from cellular to molecular. This study aimed to investigate the gene expression changes in zebrafish brains through young-adult to adult, and adult to old age. RNA sequencing was performed on isolated neuronal cells from zebrafish brains. Read More

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http://dx.doi.org/10.1016/j.neurobiolaging.2020.04.017DOI Listing

Synergistic effect of fenpropathrin and paclobutrazol on early life stages of zebrafish (Danio rerio).

Environ Pollut 2020 Jun 22;266(Pt 3):115067. Epub 2020 Jun 22.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products / Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture / Laboratory (Hangzhou) for Risk Assessment of Agricultural Products of Ministry of Agriculture, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, China. Electronic address:

Aquatic organisms are usually exposed to various co-existing pollutants. However, toxic effects of pesticide mixtures on aquatic organisms and its potential underlying mechanism still remain unclear. The joint effects of fenpropathrin (FEN) and paclobutrazol (PAC) on zebrafish (Danio rerio) using diverse toxicological endpoints were investigated in the current work. Read More

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http://dx.doi.org/10.1016/j.envpol.2020.115067DOI Listing

Human platelets use a cytosolic Ca nanodomain to activate Ca-dependent shape change independently of platelet aggregation.

Cell Calcium 2020 Jun 23;90:102248. Epub 2020 Jun 23.

School of Medicine, Keele University, Keele, Staffordshire, ST5 5BG, UK. Electronic address:

Human platelets use a rise in cytosolic Ca concentration to activate all stages of thrombus formation, however, how they are able to decode cytosolic Ca signals to trigger each of these independently is unknown. Other cells create local Ca signals to activate Ca-sensitive effectors specifically localised to these subcellular regions. However, no previous study has demonstrated that agonist-stimulated human platelets can generate a local cytosolic Ca signal. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102248DOI Listing

Natural therapeutic agents for neurodegenerative diseases from the shells of Xanthoceras sorbifolium.

Bioorg Chem 2020 Jun 24;101:104038. Epub 2020 Jun 24.

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China. Electronic address:

Neuroinflammation is linked to neurodegenerative diseases, manifested by the microglial-released over-production of nitric oxide (NO). However, so far there is no effective strategy regarding curing or preventing neurodegenerative diseases. Triterpene saponins from Xanthoceras sorbifolium were proved to be capable of eliciting a protective effect in neurodegenerative diseases. Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.104038DOI Listing

Synthesis and antitumor activity of α,β-unsaturated carbonyl moiety- containing oleanolic acid derivatives targeting PI3K/AKT/mTOR signaling pathway.

Bioorg Chem 2020 Jun 25;101:104036. Epub 2020 Jun 25.

Department of Pharmacology, Dalian Medical University, Dalian 116044, China. Electronic address:

Oleanolic acid (OA) and its semi-synthetic derivatives have been reported to have a wide range of biological activities. The introduction of electrophilic Michael acceptor group can increase the reactivity of OA to cellular targets and thus improve the anti-tumor activity. In this work, a series of novel α,β-unsaturated carbonyl derivatives of OA were designed and synthesized. Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.104036DOI Listing

Anticancer potential of new 3-nitroaryl-6-(N-methyl)piperazin-1,2,4-triazolo[3,4-a]phthalazines targeting voltage-gated K channel: Copper-catalyzed one-pot synthesis from 4-chloro-1-phthalazinyl-arylhydrazones.

Bioorg Chem 2020 Jun 19;101:104031. Epub 2020 Jun 19.

Department of Chemistry, University of Florida, Gainesville, FL, United States. Electronic address:

A series of six 3-aryl-6-(N-methylpiperazin)-1,2,4-triazolo[3,4-a]phthalazines were prepared through a facile and efficient one-pot copper-catalyzed procedure from 4-chloro-1-phthalazinyl-arylhydrazones with relatively good yields (62-83%). The one-pot copper-catalytic procedure consists of two simultaneous reactions: (i) a direct intramolecular dehydrogentaive cyclization between ylidenic carbon and adjacent pyrazine nitrogen to form 1,2,4-triazolo ring and, (ii) a direct N-amination on carbon-chlorine bond. Then, an in vitro anticancer evaluation was performed for the synthesized compounds against five selected human cancer cells (A549, MCF-7, SKBr3, PC-3 and HeLa). Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.104031DOI Listing

Synthesis of a novel fluorescent berberine derivative convenient for its subcellular localization study.

Bioorg Chem 2020 Jun 17;101:104021. Epub 2020 Jun 17.

Biological Engineering College, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250056, Shandong Province, China.

Berberine is a naturally occurred isoquinoline alkaloid that shows great potential for developing anticancer drugs. However, the problem stays of poor understanding of the mechanisms of anticancer action of berberine. It depends on evaluation of berberine's pharmacokinetics, namely monitoring of its uptake and distribution in cells, tissues and organs. Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.104021DOI Listing

Assessment of acute toxicological effects of molybdenum(IV) disulfide nano- and microparticles after single intratracheal administration in rats.

Sci Total Environ 2020 Jun 27;742:140545. Epub 2020 Jun 27.

Nofer Institute of Occupational Medicine, Łódź, Poland. Electronic address:

Despite growing applications of molybdenum(IV) sulfide (MoS) nano- and microparticles in their capacity as lubricants, data available on their safety are scarce. In this study the effect of MoS nano- and microparticles after single intratracheal instillation in rats has been analyzed. MoS suspensions were administered at the dose of 1. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.140545DOI Listing

Intergenerational transmission of childhood trauma? Testing cellular aging in mothers exposed to sexual abuse and their children.

Psychoneuroendocrinology 2020 Jun 27;120:104781. Epub 2020 Jun 27.

Department of Biobehavioral Health, The Pennsylvania State University, University Park, PA, USA. Electronic address:

Background: Exposure to maltreatment in childhood can lead to increased risk for poor health outcomes in adulthood. Child maltreatment and later poor health may be linked by premature biological aging. We tested whether childhood sexual abuse (CSA) was associated with telomere length (TL) in adult females. Read More

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http://dx.doi.org/10.1016/j.psyneuen.2020.104781DOI Listing

Autonomic and cardiovascular consequences resulting from experimental hemorrhagic stroke in the left or right intermediate insular cortex in rats.

Auton Neurosci 2020 Jun 24;227:102695. Epub 2020 Jun 24.

Departamento de Fisiologia & Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:

Damage to the insular cortex (IC) results in serious cardiovascular consequences and evidence indicates that the characteristics are lateralized. However, a study comparing the effects of focal experimental hemorrhage between IC sides was never performed. We compared the cardiovascular, autonomic and cardiac changes produced by focal experimental hemorrhage (ICH) into the left (L) or right (R) IC. Read More

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http://dx.doi.org/10.1016/j.autneu.2020.102695DOI Listing

Prevention of heart failure with preserved ejection fraction (HFpEF): reexamining microRNA-21 inhibition in the era of oligonucleotide-based therapeutics.

Cardiovasc Pathol 2020 May 19;49:107243. Epub 2020 May 19.

The University of Texas Health Science Center at Houston, McGovern Medical School.

Heart failure with preserved ejection fraction (HFpEF) accounts for 50% of cases of heart failure, which is the most common cause of hospitalization in US patients over the age of 65. HFpEF pathogenesis is increasingly believed to be due to pathological hypertrophy and fibrosis of the myocardium that may be a result of systemic inflammation from comorbid conditions such as hypertension, diabetes mellitus, chronic obstructive pulmonary disease, anemia, chronic kidney disease and others. It is believed that oxidative stress triggers a process of pathological hypertrophy and fibrosis in cardiac endothelial cells, which leads to increased left ventricle filling pressures and, eventually, symptoms of heart failure. Read More

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http://dx.doi.org/10.1016/j.carpath.2020.107243DOI Listing

Upregulation of miR-200c-3p induced by NaF promotes endothelial apoptosis by activating Fas pathway.

Environ Pollut 2020 Jun 26;266(Pt 1):115089. Epub 2020 Jun 26.

Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, 150081, Heilongjiang Province, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Harbin Medical University, Harbin, 150081, Heilongjiang Province, China; Institution of Environmentally Related Diseases, Harbin Medical University, Harbin, Heilongjiang Province, China. Electronic address:

Fluoride has been considered as a risk factor of cardiovascular disease due to its endothelial toxicology. However, the mechanism underlying the endothelial toxicity of fluoride has not been clearly illustrated. MiR-200c-3p was strongly linked with endothelial function and its level is increased in serum of fluorosis patients, but it is unclear the role of miR-200c-3p in the fluoride induced endothelial dysfunction. Read More

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http://dx.doi.org/10.1016/j.envpol.2020.115089DOI Listing

Naphthylisoquinoline alkaloids and their synthetic analogs as potent novel inhibitors against Babesia canis in vitro.

Vet Parasitol 2020 Jun 26;283:109177. Epub 2020 Jun 26.

Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul. Głęboka 30, 20-612, Lublin, Poland. Electronic address:

Babesia canis is the predominant and clinically relevant canine Babesia species in Europe. Transmitted by vector ticks, the parasite enters red blood cells and induces a severe, potentially fatal hemolytic anemia. Here, we report on the antibabesial activities of three extracts of the West African tropical plant species Triphyophyllum peltatum (Dioncophyllaceae) and Ancistrocladus abbreviatus (Ancistrocladaceae) and of 13 genuine naphthylisoquinoline alkaloids isolated thereof. Read More

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http://dx.doi.org/10.1016/j.vetpar.2020.109177DOI Listing

Engineering the Cellular Mechanical Microenvironment to Regulate Stem Cell Chondrogenesis: Insights from a Microgel Model.

Acta Biomater 2020 Jul 3. Epub 2020 Jul 3.

Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510006, China; National Engineering Research Center for Tissue Restoration and Reconstruction (NERC-TRR), Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, P. R. China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510641, China. Electronic address:

Biophysical cues (especially mechanical cues) embedded in cellular microenvironments show a critical impact on stem cell fate. Despite the capability of traditional hydrogels to mimic the feature of extracellular matrix (ECM) and tune their physicochemical properties via diverse approaches, their relatively large size not only induces biased results, but also hinders high-throughput screening and analysis. In this paper, a microgel model is proposed to recapitulate the role of 3D mechanical microenvironment on stem cell behaviors especially chondrogenesis in vitro. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.06.046DOI Listing

BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases.

Neoplasia 2020 Jul 3;22(9):376-389. Epub 2020 Jul 3.

The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA; Masonic Cancer Center, 2231 6th St SE, Minneapolis, MN 5545, USA. Electronic address:

The development of mutant BRAF inhibitors has improved the outcome for melanoma patients with BRAF mutations. Although the initial response to these inhibitors can be dramatic, sometimes resulting in complete tumor regression, the majority of melanomas become resistant. To study resistance to BRAF inhibition, we developed a novel mouse model of melanoma using a tetracycline/doxycycline-regulated system that permits control of mutant BRAF expression. Read More

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http://dx.doi.org/10.1016/j.neo.2020.06.006DOI Listing

Dual inhibition of VEGF and PARP suppresses KRAS-mutant colorectal cancer.

Neoplasia 2020 Jul 3;22(9):365-375. Epub 2020 Jul 3.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China. Electronic address:

The addition of bevacizumab to chemotherapy has prolonged overall and progression-free survival rates for metastatic colorectal cancer (mCRC). However, KRAS-mutant (KRAS-mut) CRC, lacking an ideal targeted agent, represents an inferior-response subgroup of patients. In the present study, we investigated a combination approach of bevacizumab + olaparib in KRAS-mut CRC in a preclinical setting. Read More

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http://dx.doi.org/10.1016/j.neo.2020.06.001DOI Listing

IcyHot analgesic topical cream limits cardiac injury in rodents.

Transl Res 2020 Jul 3. Epub 2020 Jul 3.

Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA 94305, USA. Electronic address:

Little is known whether topical analgesic creams, whose natural products enter the blood stream after application, affect myocardial infarct size. Here we tested whether topical analgesic creams can trigger remote cardioprotection and the mechanism involved. Male Sprague Dawley rats were used for an in vivo rodent model consisting of 30 minutes left anterior descending coronary artery ischemia and 2 hours of reperfusion followed by infarct size assessment. Read More

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http://dx.doi.org/10.1016/j.trsl.2020.06.009DOI Listing

Role of Prx1-expressing skeletal cells and Prx1-expression in fracture repair.

Bone 2020 Jul 3:115521. Epub 2020 Jul 3.

Department of Orthopaedic Surgery, Section of Molecular Medicine, Rush University Medical Center, Chicago, IL, USA; Department of Pediatrics, Division of Pediatric Endocrinology, Rush University Medical Center, Chicago, IL, USA. Electronic address:

The healing capacity of bones after fracture implies the existence of adult regenerative cells. However, information on identification and functional role of fracture-induced progenitors is still lacking. Paired-related homeobox 1 (Prx1) is expressed during skeletogenesis. Read More

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http://dx.doi.org/10.1016/j.bone.2020.115521DOI Listing

Modulating the modulators: regulation of protein arginine methyltransferases by post-translational modifications.

Drug Discov Today 2020 Jul 3. Epub 2020 Jul 3.

Department of Pharmacology and Toxicology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA; Department of Medical and Molecular Genetics, 975 W. Walnut Street, Indianapolis, IN 46202, USA. Electronic address:

The therapeutic potential of targeting protein arginine methyltransferases (PRMTs) is inextricably linked to their key roles in various cellular functions, including splicing, proliferation, cell cycle regulation, differentiation, and DNA damage signaling. Unsurprisingly, the development of inhibitors against these enzymes has become a rapidly expanding research area. However, effective targeting of PRMTs requires a deeper understanding of the mechanistic details behind their regulation at multiple levels, involving those mechanisms that alter their activity, interactions, and localization. Read More

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http://dx.doi.org/10.1016/j.drudis.2020.06.031DOI Listing

Repositioning chloroquine as antiviral prophylaxis against COVID-19: potential and challenges.

Drug Discov Today 2020 Jul 3. Epub 2020 Jul 3.

Institute of East-West Medicine, New York, NY, USA; National Taiwan University Hospital, Taipei, Taiwan.

The Coronavirus Disease 2019 (COVID-19) pandemic is advancing globally, and pharmaceutical prophylaxis is one solution. Here, we propose repositioning chloroquine (CQ) as prophylaxis against COVID-19. CQ blocks viral attachment and entry to host cells and demonstrates efficacy against a variety of viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19. Read More

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http://dx.doi.org/10.1016/j.drudis.2020.06.030DOI Listing

Systemic Iron Overload Exacerbates Osteoarthritis in the Strain 13 Guinea Pig.

Osteoarthritis Cartilage 2020 Jul 3. Epub 2020 Jul 3.

Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA. Electronic address:

Objective: Iron is emerging as a key player in aging-associated diseases due to its propensity for driving free radical formation. Studies examining the role of iron in the pathogenesis of primary osteoarthritis (OA) are limited. Our objective was to establish a direct relationship between excess iron and OA by administering iron dextran to a guinea pig strain with decreased propensity for developing OA. Read More

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http://dx.doi.org/10.1016/j.joca.2020.06.005DOI Listing

Quantifying drug-induced structural toxicity in hepatocytes and cardiomyocytes derived from hiPSCs using a deep learning method.

J Pharmacol Toxicol Methods 2020 Jul 3:106895. Epub 2020 Jul 3.

Division of Applied Regulatory Science, Office of Translational Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA. Electronic address:

Cardiac and hepatic toxicity result from induced disruption of the functioning of cardiomyocytes and hepatocytes, respectively, which is tightly related to the organization of their subcellular structures. Cellular structure can be analyzed from microscopy imaging data. However, subtle or complex structural changes that are not easily perceived may be missed by conventional image-analysis techniques. Read More

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http://dx.doi.org/10.1016/j.vascn.2020.106895DOI Listing

Low Non-Relapse Mortality after HLA Matched Related Two-Step Hematopoietic Stem Cell Transplantation using Cyclophosphamide (CY) for Graft versus Host Disease Prophylaxis and the Potential Impact of Non-CY-Exposed T Cells on Outcomes.

Biol Blood Marrow Transplant 2020 Jul 3. Epub 2020 Jul 3.

Department of Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University.

The use of cyclophosphamide (CY) for bidirectional tolerization of recipient and donor T cells is associated with reduced rates of graft versus host disease (GVHD) and non-relapse mortality (NRM) after human leukocyte antigen (HLA) matched hematopoietic stem cell transplantation (HSCT). However, recurrent disease remains the primary barrier to long term survival. We extended our two-step approach to HLA matched related HSCT using a radiation-based myeloablative conditioning regimen combined with a high dose of T cells in an attempt to reduce relapse rates while maintaining the beneficial effects of CY tolerization. Read More

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http://dx.doi.org/10.1016/j.bbmt.2020.06.021DOI Listing
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