5,578,571 results match your criteria Cellular physiology and biochemistry : international journal of experimental cellular physiology biochemistry and pharmacology[Journal]

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PTEN-deficient prostate cancer is associated with an immunosuppressive tumor microenvironment mediated by increased expression of IDO1 and infiltrating FoxP3+ T regulatory cells.

Prostate 2019 Apr 18. Epub 2019 Apr 18.

Cancer Biology and Genetics, Queen's Cancer Research Institute, Queen's University, Kingston, Canada.

Background: Accumulating evidence shows that tumor cell-specific genomic changes can influence the cross talk between cancer cells and the surrounding tumor microenvironment (TME). Loss of the PTEN tumor suppressor gene is observed in 20% to 30% of prostate cancers (PCa) when first detected and the rate increases with PCa progression and advanced disease. Recent findings implicate a role for PTEN in cellular type I interferon response and immunosuppression in PCa. Read More

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http://dx.doi.org/10.1002/pros.23808DOI Listing

Effect of Homeopathic Medicines on Intestinal Coccidia and Immune Response Cells in Spotted Rose Snapper (Lutjanus guttatus).

Homeopathy 2019 Apr 18. Epub 2019 Apr 18.

Centro de Investigaciones Biológicas del Noroeste S. C. (CIBNOR), La Paz, México.

Background:  Homeopathy has been widely applied in freshwater species but rarely in marine fish. Farm stress destabilises host-pathogen equilibrium, favouring parasites and disease. Coccidian endoparasites cause intestinal infections and cell degeneration. Read More

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http://dx.doi.org/10.1055/s-0039-1681062DOI Listing

Normal Visual Recovery after Optic Neuritis Despite Significant Loss of Retinal Ganglion Cells in Patients with Multiple Sclerosis.

Klin Monbl Augenheilkd 2019 Apr 18;236(4):425-428. Epub 2019 Apr 18.

Department of Ophthalmology, University of Lausanne, Jules-Gonin Eye Hospital, Lausanne, Switzerland.

Background: Optic neuritis (ON) is a frequent manifestation of demyelinating attack in multiple sclerosis (MS). Initial visual loss can vary from minimal to complete. Visual improvement occurs in about 95% of patients, some of them recovering to normal [visual acuity (VA), color vision, visual field (VF)]. Read More

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http://dx.doi.org/10.1055/a-0853-1721DOI Listing

A Rhesus Monkey With a Naturally Occurring Impairment of Disparity Vergence. II. Abnormal Near Response Cell Activity in the Supraoculomotor Area.

Invest Ophthalmol Vis Sci 2019 Apr;60(5):1670-1676

Washington National Primate Research Center, University of Washington, Seattle, Washington, United States.

Purpose: Convergence insufficiency is a very common disorder that can have significant adverse effects on school performance. When reading, children with this disorder often experience diplopia and headaches. We have recently obtained a rhesus monkey with a naturally occurring impairment of vergence eye movements. Read More

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http://dx.doi.org/10.1167/iovs.18-26440DOI Listing

Role of Nodal signalling in testis development and initiation of testicular cancer.

Reproduction 2019 Apr 1. Epub 2019 Apr 1.

A Jorgensen, Department of Growth and Reproduction, Rigshospitalet, Kobenhavn, Denmark.

Testicular development from the initially bipotential gonad is a tightly regulated process involving a complex signalling cascade to ensure proper sequential expression of signalling factors and secretion of steroid hormones. Initially, Sertoli cell specification facilitates differentiation of the steroidogenic fetal Leydig cells and establishment of the somatic niche, which is critical in supporting the germ cell population. Impairment of the somatic niche during fetal life may lead to development of male reproductive disorders, including arrest of gonocyte differentiation, which is considered the first step in the testicular cancer pathogenesis. Read More

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http://dx.doi.org/10.1530/REP-18-0641DOI Listing

Screening for mutations in selected miRNA genes in hypogonadotropic hypogonadism patients.

Endocr Connect 2019 May;8(5):506-509

Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland.

In approximately half of congenital hypogonadotropic hypogonadism (cHH) patients, the genetic cause remains unidentified. Since the lack of certain miRNAs in animal models has led to cHH, we sequenced human miRNAs predicted to regulate cHH-related genes (MIR7-3, MIR141, MIR429 and MIR200A-C) in 24 cHH patients with Sanger sequencing. A heterozygous variant in MIR200A (rs202051309; general population frequency of 0. Read More

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http://dx.doi.org/10.1530/EC-19-0080DOI Listing

MADD silencing enhances anti-tumor activity of TRAIL in anaplastic thyroid cancer.

Endocr Relat Cancer 2019 Jun;26(6):551-563

Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois, USA.

ATC is an aggressive disease with limited therapeutic options due to drug resistance. TRAIL is an attractive anti-cancer therapy that can trigger apoptosis in a cancer cell-selective manner. However, TRAIL resistance is a major clinical obstacle for its use as a therapeutic drug. Read More

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https://erc.bioscientifica.com/view/journals/erc/26/6/ERC-18
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http://dx.doi.org/10.1530/ERC-18-0517DOI Listing
June 2019
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Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation.

Stem Cell Res 2019 Apr 10;37:101434. Epub 2019 Apr 10.

Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA. Electronic address:

Patient-derived induced pluripotent stem cells (iPSCs) have become a promising resource for exploring genetics of complex diseases, discovering new drugs, and advancing regenerative medicine. Increasingly, laboratories are creating their own banks of iPSCs derived from diverse donors. However, there are not yet standardized guidelines for qualifying these cell lines, i. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101434DOI Listing

Multiple distinct O-Mannosylation pathways in eukaryotes.

Curr Opin Struct Biol 2019 Apr 15;56:171-178. Epub 2019 Apr 15.

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. Electronic address:

Protein O-mannosylation (O-Man), originally discovered in yeast five decades ago, is an important post-translational modification (PTM) conserved from bacteria to humans, but not found in plants or nematodes. Until recently, the homologous family of ER-located protein O-mannosyl transferases (PMT1-7 in yeast; POMT1/POMT2 in humans), were the only known enzymes involved in directing O-Man biosynthesis in eukaryotes. However, recent studies demonstrate the existence of multiple distinct O-Man glycosylation pathways indicating that the genetic and biosynthetic regulation of O-Man in eukaryotes is more complex than previously envisioned. Read More

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http://dx.doi.org/10.1016/j.sbi.2019.03.003DOI Listing

Contamination of a hospital plumbing system by persister cells of a copper-tolerant high-risk clone of Pseudomonas aeruginosa.

Water Res 2019 Apr 10;157:579-586. Epub 2019 Apr 10.

Laboratoire d'Hygiène Hospitalière, Centre Hospitalier Régional Universitaire, Besançon, France; UMR CNRS 6249, Chrono-environnement, Université de Bourgogne Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Filière Microbiologique de Besançon, Centre Hospitalier Régional Universitaire, Besançon, France. Electronic address:

Background: Pseudomonas aeruginosa (PA) is an important opportunistic pathogen that thrives best in the distal elements of plumbing and waste-water systems. Although nosocomial outbreaks of PA have been associated with water sources, the role of the plumbing system of healthcare premises as a reservoir for this pathogen is still unclear.

Materials And Methods: We collected water samples from 12 technical areas, distant from any medical activity, in a teaching hospital in France once a week for 11 weeks. Read More

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http://dx.doi.org/10.1016/j.watres.2019.04.011DOI Listing

Vieloplains A-G, seven new guaiane-type sesquiterpenoid dimers from Xylopia vielana.

Bioorg Chem 2019 Apr 10;88:102891. Epub 2019 Apr 10.

Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China; Institute of Interdisciplinary Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China; School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address:

Seven new guaiane-type sesquiterpene dimers vieloplains A-G, connecting patterns through three different direct CC bonds compounds 1-5 (C-3 to C-3', C-4 to C-1'), compound 6 (C-2 to C-3', C-4 to C-2') and compound 7 (C-2 to C-1', C-4 to C-2') were isolated from the roots of Xylopia vielana. Their absolute configurations were established by NOESY analysis, the Cu Kα X-ray crystallographic the experiment circular dichroism (ECD) and the calculated ECD. Among them, only compound 6 showed a considerable cytotoxicity against DU145 cells with IC values of 9. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.03.065DOI Listing

Sampling and multiplexing in lab-on-paper bioelectroanalytical devices for glucose determination.

Biosens Bioelectron 2019 Apr 5;135:64-70. Epub 2019 Apr 5.

Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006, Oviedo, Spain. Electronic address:

In this work, we present a multiplexed (eight simultaneous measurements) paper-based electrochemical device developed in a very simple way and using low-cost materials, such as paper, carbon ink and multifunctional connector headers. Meanwhile, we have also combined the paper-based electrochemical platform with a glass-fiber strip in order to integrate easily a sampling step. Both approaches, simultaneous measuring and sampling, have been applied to the determination of glucose using bienzymatic biosensors. Read More

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http://dx.doi.org/10.1016/j.bios.2019.04.006DOI Listing

Discovery of 7H-pyrrolo[2,3-d]pyrimidine derivatives as selective covalent irreversible inhibitors of interleukin-2-inducible T-cell kinase (Itk).

Eur J Med Chem 2019 Mar 26;173:167-183. Epub 2019 Mar 26.

State Key Laboratory of Chemical Oncogenomics, Engineering Laboratory for Chiral Drug Synthesis, School of Chemical Biology and Biotechnology, Shenzhen Graduate School, Peking University, Shenzhen, 518055, China. Electronic address:

Interleukin-2-inducible T-cell kinase (Itk) plays an important role in multiple signal transduction pathways in T and mast cells, and is a potential drug target for treating inflammatory diseases, autoimmune diseases, and T cell leukemia/lymphoma. Herein, we describe the discovery of a series of covalent Itk inhibitors based on the 7H-pyrrolo[2,3-d]pyrimidine scaffold. Placing an appropriate substitution group at a hydration site of the ATP binding pocket of Itk and using a saturated heterocyclic ring as a linker to the reactive group were crucial for selectivity. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.03.055DOI Listing

A method for the cell-cycle-specific analysis of radiation-induced chromosome aberrations and breaks.

Mutat Res 2019 Apr 6;815:10-19. Epub 2019 Apr 6.

Institute of Medical Radiation Biology, University of Duisburg-Essen Medical School, Essen, Germany. Electronic address:

The classical G-assay is widely used to assess cell-radiosensitivity and cancer phenotype: Cells are exposed to low doses of ionizing-radiation (IR) and collected for cytogenetic- analysis ˜1.5 h later. In this way, chromosome-damage is measured in cells irradiated in G-phase, without retrieving information regarding kinetics of chromosome-break-repair. Read More

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http://dx.doi.org/10.1016/j.mrfmmm.2019.04.001DOI Listing

Plant cell division - defining and finding the sweet spot for cell plate insertion.

Authors:
Sabine Müller

Curr Opin Cell Biol 2019 Apr 15;60:9-18. Epub 2019 Apr 15.

Center for Plant Molecular Biology, Auf der Morgenstelle 32, 72076 Tübingen, Germany. Electronic address:

The plant microtubules form unique arrays using acentrosomal microtubule nucleation pathways, yet utilizing evolutionary conserved centrosomal proteins. In cytokinesis, a multi-component cytoskeletal apparatus, the phragmoplast mediates the biosynthesis of the new cell plate by dynamic centrifugal expansion, a process that demands exquisite coordination of microtubule turnover and endomembrane trafficking. At the same time, the phragmoplast is guided to meet with the parental wall at a cortical site that is predefined before mitotic entry and transiently marked by the preprophase band of microtubules. Read More

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http://dx.doi.org/10.1016/j.ceb.2019.03.006DOI Listing

Comparability of biological aging measures in the National Health and Nutrition Examination Study, 1999-2002.

Psychoneuroendocrinology 2019 Apr 4;106:171-178. Epub 2019 Apr 4.

Department of Epidemiology, Columbia University Mailman School of Public Health, United States; Robert N. Butler Columbia Aging Center, Columbia University, United States. Electronic address:

Background: Biological processes of aging are thought to be modifiable causes of many different chronic diseases. Measures of biological aging could provide sensitive endpoints for studies of risk factors hypothesized to shorten healthy lifespan and/or interventions that extend it. But uncertainty remains about how to measure biological aging and if proposed measures assess the same thing. Read More

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http://dx.doi.org/10.1016/j.psyneuen.2019.03.012DOI Listing

G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands.

J Inorg Biochem 2019 Apr 6;196:110681. Epub 2019 Apr 6.

Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai 200092, PR China. Electronic address:

In this paper, three new Ruthenium(II) polypyridyl complexes containing ascididemin (ASC) as main ligand have been synthesized and characterized. Their interactions with different G-quadruplex (Htelo, c-myc and c-kit) (Htelo: human telomeric DNA, c-myc: cellular-myelocytomatosis viral oncogene, c-kit: oncogene c-kit promoter sequences) and duplex (ds26) DNA sequences were comparatively studied with the free ligand ASC by a series of spectroscopic techniques including UV-vis (ultraviolet-visible) spectroscopy, FID (fluorescent intercalator displacement) assay, and FRET (fluorescence resonance energy transfer) melting assay. Molecular docking studies were also performed to support the binding mode of the compounds with G-quadruplex DNA. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2019.03.021DOI Listing

Dopamine induces release of calcium from internal stores in layer II lateral entorhinal cortex fan cells.

Cell Calcium 2019 Apr 10;80:103-111. Epub 2019 Apr 10.

Center for Studies in Behavioral Neurobiology, Department of Psychology, Concordia University, Montréal, Québec, H4B 1R6, Canada. Electronic address:

The entorhinal cortex plays an important role in temporal lobe processes including learning and memory, object recognition, and contextual information processing. The alteration of the strength of synaptic inputs to the lateral entorhinal cortex may therefore contribute substantially to sensory and mnemonic functions. The neuromodulatory transmitter dopamine exerts powerful effects on excitatory glutamatergic synaptic transmission in the entorhinal cortex. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.04.003DOI Listing

Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts.

J Mech Behav Biomed Mater 2019 Apr 10;95:143-152. Epub 2019 Apr 10.

Department of Physics, UNESP, São Paulo State University, 17033-360, Bauru, SP, Brazil.

Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. Read More

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http://dx.doi.org/10.1016/j.jmbbm.2019.04.003DOI Listing

Baicalein attenuates pancreatic inflammatory injury through regulating MAPK, STAT 3 and NF-κB activation.

Int Immunopharmacol 2019 Apr 15;72:204-210. Epub 2019 Apr 15.

Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address:

Acute pancreatitis (AP) is a common acute abdominal disease with local or systemic inflammatory response, caused by abnormal activation of digestive enzymes. Baicalein has been shown to exert anti-inflammatory effects and to attenuate the pathological changes of AP. The aim of the research was to investigate the effects of baicalein on caerulein induced pancreatitis, and to elucidate the putative underlying mechanism. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.04.018DOI Listing

Effects of perfluorooctanoic acid on stem Leydig cell functions in the rat.

Environ Pollut 2019 Apr 9;250:206-215. Epub 2019 Apr 9.

Department of Pediatrics, Peking University First Hospital, No.1 Xi'an Men Street, West District, Beijing, 100034, China; Department of Pediatrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China. Electronic address:

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic (PFOS) are two perfluorinated chemical products widely existing in the environment. Evidence suggested that PFOA might relate to male reproductive dysfunction in rats and humans. PFOA exposure inhibited the function of Leydig cells. Read More

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http://dx.doi.org/10.1016/j.envpol.2019.03.120DOI Listing

Increasing DNA content for cost-effective oil production in Parachlorella kessleri.

Bioresour Technol 2019 Apr 11;285:121332. Epub 2019 Apr 11.

State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China; Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

The aim of this work was to study salt stress effects on DNA content and oil production processes integrating harvesting, lipid accumulation and oil extraction. Salt-induced enlargement of Parachlorella kessleri cells, with increasing content of DNA and neutral lipid were found. The 34. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.121332DOI Listing

Immobilization of recombinant Escherichia coli whole cells harboring xylose reductase and glucose dehydrogenase for xylitol production from xylose mother liquor.

Bioresour Technol 2019 Apr 13;285:121344. Epub 2019 Apr 13.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China.

In this study, recombinant E. coli BL21(DE3)/pCDFDuet-1-XR-GDH harboring xylose reductase (XR) and glucose dehydrogenase (GDH) were immobilized and applied for the production of xylitol from xylose mother liquor (XML). Various immobilization methods were screened and the cross-linking approach with diatomite and polyetherimide as the raw materials and glutaraldehyde as the cross-linking agent was the optimal one, and the recovery activity reached of 80. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.121344DOI Listing

Combined effects of environmental xeno-estrogens within multi-component mixtures: Comparison of in vitro human- and zebrafish-based estrogenicity bioassays.

Chemosphere 2019 Apr 9;227:334-344. Epub 2019 Apr 9.

Institut National de l'Environnement Industriel et des risques (INERIS), Unité Ecotoxicologie in vitro et in vivo, UMR-I 02 SEBIO, 60550, Verneuil-en-Halatte, France. Electronic address:

Some recent studies showed that in vitro bioassays based on fish or human estrogen receptor (ER) activation may have distinct responses to environmental samples, highlighting the need to better understand bioassay-specific ER response to environmental mixtures. For this purpose, we investigated a 12-compound mixture in two mixture ratios (M1 and M2) on zebrafish (zf) liver cells stably expressing zfERα (ZELHα cells) or zfERβ2 (ZELHβ2 cells) and on human ER-reporter gene (MELN) cells. The mixture included the well-known ER ligands bisphenol A (BPA) and genistein (GEN), and other compounds representatives of a freshwater background contamination. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.04.060DOI Listing

Photobiomodulation associated to cellular therapy improve wound healing of experimental full thickness burn wounds in rats.

J Photochem Photobiol B 2019 Apr 10;194:174-182. Epub 2019 Apr 10.

Laboratory of Experimental Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil. Electronic address:

Adipose derived stromal vascular fraction (SVF) is a method of cell therapy potentially applicable for treatment of full thickness burns. Here we investigated if the association of photobiomodulation (PBM) with SVF therapy could improve wound healing in experimentally induced full thickness burn wounds in rats compared to the topical agent 2% silver sulfadiazine in a dose-dependent manner. Sixty-six male Wistar rats were divided in 4 groups containing 5 animals each which received the following treatments: 2% sulfadiazine (SD), SVF, SVF plus PBM at 30 mW (SVFL30), and SVF plus PBM at 100 mW (SVFL100). Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.04.003DOI Listing

Molecular cloning and mRNA expression of IFIT5 in tissues of ducklings infected with virulent duck hepatitis A virus type 3.

Res Vet Sci 2019 Apr 8;124:256-262. Epub 2019 Apr 8.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, PR China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Interferon-induced proteins with tetratricopeptide repeats (IFITs) are a family of proteins strongly induced downstream of type I interferon signaling. The function of IFITs has been investigated extensively in mammals. IFIT5 is the sole protein in this family found in birds and little information is available about the function of avian IFIT5. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00345288183132
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http://dx.doi.org/10.1016/j.rvsc.2019.04.003DOI Listing
April 2019
1 Read

Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.

Virology 2019 Apr 3;532:22-29. Epub 2019 Apr 3.

Infection Biology Unit, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany; Faculty of Biology and Psychology, Wilhelm-Weber-Str. 2, University Göttingen, 37073 Göttingen, Germany. Electronic address:

Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated entry of retroviral-, rhabdoviral and filoviral particles depends on CatB/CatL activity and that this effect is cell line-independent. Read More

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http://dx.doi.org/10.1016/j.virol.2019.03.020DOI Listing

Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children.

Int J Pediatr Otorhinolaryngol 2019 Apr 11;122:111-116. Epub 2019 Apr 11.

Department of Pediatrics, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, Brno, Czech Republic.

Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma.

Study Design: a prospective study analysing 31 surgically removed retraction pockets. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01655876193017
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http://dx.doi.org/10.1016/j.ijporl.2019.04.008DOI Listing
April 2019
1 Read

Superparamagnetic iron oxide nanoparticles of curcumin enhance gemcitabine therapeutic response in pancreatic cancer.

Biomaterials 2019 Apr 8;208:83-97. Epub 2019 Apr 8.

Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, USA. Electronic address:

Pancreatic cancer is a complex disease accounting for fibrotic tumors and an aggressive phenotype. Gemcitabine (GEM) is used as a standard therapy, which develops chemoresistance leading to poor patient outcome. We have recently developed a superparamagnetic iron oxide nanoparticle (SPION) formulation of curcumin (SP-CUR), which is a nontoxic, bioactive anti-inflammatory/anti-cancer agent for its enhanced delivery in tumors. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.04.005DOI Listing

Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration.

Biomaterials 2019 Apr 9;208:72-82. Epub 2019 Apr 9.

HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park, Nanshan, Shenzhen, 518057, China; Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Institute of Molecular Functional Materials, And Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, 999077, China; Center for Aggregation-Induced Emission, SCUT-HKUST Joint Research Institute, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640, China. Electronic address:

Photostability is a particularly important parameter for fluorescence imaging especially long-term dynamic tracking in live samples. However, many organic fluorophores show poor photostability under one-photon and two-photon continuous irradiation. In addition, these traditional fluorophores also suffer from aggregation-caused quenching (ACQ) in aggregate state in insolvable water environment. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.04.002DOI Listing

Low-dose cadmium potentiates lung inflammatory response to 2009 pandemic H1N1 influenza virus in mice.

Environ Int 2019 Apr 15;127:720-729. Epub 2019 Apr 15.

Division of Pulmonary, Allergy and Critical Care Medicine, Emory University School of Medicine, Atlanta, GA 30322, United States of America. Electronic address:

Cadmium (Cd) is a toxic, pro-inflammatory metal ubiquitous in the diet that accumulates in body organs due to inefficient elimination. Responses to influenza virus infection are variable, particularly severity of pneumonia. We used a murine model of chronic low-dose oral exposure to Cd to test if increased lung tissue Cd worsened inflammation in response to sub-lethal H1N1 infection. Read More

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http://dx.doi.org/10.1016/j.envint.2019.03.054DOI Listing

Akebia Saponin D inhibits the formation of atherosclerosis in ApoE mice by attenuating oxidative stress-induced apoptosis in endothelial cells.

Atherosclerosis 2019 Apr 10;285:23-30. Epub 2019 Apr 10.

Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China. Electronic address:

Background And Aims: Akebia Saponin D (ASD) is a major bioactive triterpenoid saponin compound isolated from the Chinese herb Dipsacus asper wall (DSW). DSW has been long used as an anti-Alzheimer disease and anti-osteoporosis agent in clinics. However, anti-atherosclerotic effects of ASD have not been fully investigated. Read More

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http://dx.doi.org/10.1016/j.atherosclerosis.2019.04.202DOI Listing

In-vitro blood and vascular compatibility of sirolimus-eluting organic/inorganic hybrid stent coatings.

Colloids Surf B Biointerfaces 2019 Apr 9;179:405-413. Epub 2019 Apr 9.

Research Institute of Advanced Manufacturing Technology, Korea Institute of Industrial Technology, Incheon, 21999, Republic of Korea. Electronic address:

The surface characteristics of coronary stents play a pivotal role in inhibiting in-stent restenosis and late-stent thrombosis. In this study, a sol-gel-derived silica xerogel-chitosan hybrid coating was applied to Co-Cr stent and was reported, for the first time, as a biocompatible drug delivery tool in vascular stent application. A dense and uniform chitosan-silica xerogel hybrid coating (<1-μm thick) was applied on bare Co-Cr material. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.018DOI Listing

Formulation, clinical and histopathological assessment of microemulsion based hydrogel for UV protection of skin.

Colloids Surf B Biointerfaces 2019 Apr 8;179:393-404. Epub 2019 Apr 8.

Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, P.O. Box: 91388-13944, Islamic Republic of Iran.

The aim of this study was to prepare a microemulsion based hydrogel containing sesame oil and evaluate its topical application in preventing the harmful effects of UV radiation on the guinea pig's skin using histopathologic and clinical findings. Sesame oil with high antioxidant content and unique chemical and physiological properties is a suitable candidate for the evolution of UV protection on skin. Applying this natural oil in microemulsion formulation containing particles with nanometer size can enhance its efficacy. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.015DOI Listing

Strontium-incorporated mineralized PLLA nanofibrous membranes for promoting bone defect repair.

Colloids Surf B Biointerfaces 2019 Apr 6;179:363-373. Epub 2019 Apr 6.

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address:

Mineralized scaffolds, which are fabricated by electrodeposition, mimic the chemistry of natural bone and have attracted a great amount of attention due to their rapid and simple production. In this study, mineralized electrospun polylactic acid (PLLA) nanofibrous membranes containing different amounts of strontium (Sr) were fabricated by an electrodeposition method for potential use in bone regeneration applications. In vitro assays, including cell proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) grown on these membranes and in vivo cranial bone defect repair assays, were carried out. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.011DOI Listing

MiR-219a-5p enhances cisplatin sensitivity of human non-small cell lung cancer by targeting FGF9.

Biomed Pharmacother 2019 Apr 15;114:108662. Epub 2019 Apr 15.

Department of Cardiothoracic Surgery, Hwamei Hospital, University of Chinese Academy Of Science , Ningbo, Zhejiang, China.

Cisplatin (DDP) resistance is a major obstacle in the treatment of non-small cell lung cancer (NSCLC). MicroRNA-219a-5p (miR-219a-5p) has been reported to be a tumor suppressor in several cancers, but whether it regulates chemosensitivity in NSCLC remains unclear. Here, using quantitative real time PCR analysis, we observed that miR-219a-5p was down-regulated in responding tumor tissues compared with that in non-responding tumor tissues from NSCLC patients received DDP-based chemotherapy. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108662DOI Listing

Autophagy represses fascaplysin-induced apoptosis and angiogenesis inhibition via ROS and p8 in vascular endothelia cells.

Biomed Pharmacother 2019 Apr 15;114:108866. Epub 2019 Apr 15.

School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.

Fascaplysin is a natural product isolated from marine sponges that exhibits broad anticancer activity. Previous studies revealed that fascaplysin-induced apoptosis and angiogenesis inhibition in vascular endothelial cells contributed to its anticancer activity. Accumulating evidence indicates that autophagy plays a significant role in mediating the function of vascular endothelial cells (VECs) and the response to cancer therapy. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108866DOI Listing

UCA1 promotes cell viability, proliferation and migration potential through UCA1/miR-204/CCND2 pathway in primary cystitis glandularis cells.

Biomed Pharmacother 2019 Apr 15;114:108872. Epub 2019 Apr 15.

Reproductive Medicine Center, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan, 410008, China. Electronic address:

Cystitis glandularis (CG) is an unusual proliferative disorder of the urinary bladder. Increasing evidences demonstrated that long non-coding RNAs (lncRNAs) play important roles in a variety of cellular progresses. However, there are rarely reports about the role and underlying molecular mechanism of lncRNAs in CG. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108872DOI Listing

Multicenter evaluation of a novel ROS1 immunohistochemistry assay (SP384) for detection of ROS1 rearrangements in a large cohort of lung adenocarcinoma patients.

J Thorac Oncol 2019 Apr 15. Epub 2019 Apr 15.

Université Côte d'Azur, University Hospital Federation OncoAge, Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Nice, France;; Université Côte d'Azur, CNRS, INSERM, Institute of Research on Cancer and Ageing of Nice (IRCAN), University Hospital Federation OncoAge, Nice, France;; Université Côte d'Azur, University Hospital Federation OncoAge, Hospital-Related Biobank (BB-0033-00025), Pasteur Hospital, Nice, France;. Electronic address:

Introduction: The detection of a ROS1 rearrangement in advanced and metastatic lung adenocarcinoma (LUAD) lead to a targeted treatment with tyrosine kinase inhibitors, with favorable progression-free survival and overall survival of the patients. Thus, it is mandatory to screen for the ROS1 rearrangement in all these patients. ROS1 rearrangements can be detected using break-apart fluorescence in situ hybridization (FISH), which is the "gold standard"; however, ROS1 immunohistochemistry (IHC) can be used as a screening test since it is widely available, easy and rapid to perform, and cost-effective. Read More

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http://dx.doi.org/10.1016/j.jtho.2019.03.024DOI Listing

Molecular regulation of myoendothelial gap junctions.

Curr Opin Pharmacol 2019 Apr 15;45:16-22. Epub 2019 Apr 15.

Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, Biomedical Centre, Cardiovascular Physiology, LMU Munich and German Centre for Cardiovascular Research, Partner Site Munich Heart Alliance, Munich, Germany.

Myoendothelial gap junctions are involved in the regulation of vascular tone. The major connexins described in the vascular system are Cx37, Cx40, Cx43, and Cx45 with all but Cx45 found in myoendothelial connections. Although many reports on post-translational modifications of these connexins are available, only few groups have investigated their role in controlling myoendothelial communication and signal propagation. Read More

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http://dx.doi.org/10.1016/j.coph.2019.03.006DOI Listing

Role of DNA hypomethylation in lateral habenular nucleus in the development of depressive-like behavior in rats.

J Affect Disord 2019 Mar 22;252:373-381. Epub 2019 Mar 22.

Department of Physiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China. Electronic address:

Background: Lateral habenula nucleus (LHb) has recently been noted for its role in stress-induced depressive disorder. Yet little is known about the mechanisms by which external stimuli or depression induces pathological alteration in the LHb.

Methods: Chronic unpredictable mild stress (CUMS) was employed to model depressive-like behaviors in adult rats. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650327183242
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http://dx.doi.org/10.1016/j.jad.2019.03.062DOI Listing
March 2019
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Perioperative management of a patient with severe factor V deficiency presenting with chronic subdural hematoma: a clinical report.

World Neurosurg 2019 Apr 15. Epub 2019 Apr 15.

Department of Anaesthesiology University Hospital of Munich (LMU) Marchioninistraße 15 81377 Munich Germany.

Background: Severe factor V deficiency is an extremely rare coagulation disorder. Patients with factor V activity below 5% usually become symptomatic in early childhood.

Methods: We report the case of an 82 year old woman with incidentally diagnosed severe factor V deficiency, who developed a symptomatic chronic subdural hematoma, requiring burr-hole craniostomy. Read More

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http://dx.doi.org/10.1016/j.wneu.2019.04.080DOI Listing

Altered dynamics may drift pathological fibrillization in membraneless organelles.

Biochim Biophys Acta Proteins Proteom 2019 Apr 15. Epub 2019 Apr 15.

MTA-DE Laboratory of Protein Dynamics, University of Debrecen, Hungary. Electronic address:

Protein phase transition can generate non-membrane bound cellular compartments, which can convert from liquid-like to solid-like states. While the molecular driving forces of phase separation have been largely understood, much less is known about the mechanisms of material-state conversion. We apply a recently developed algorithm to describe the weak interaction network of multivalent motifs, and simulate the effect of pathological mutations. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.005DOI Listing

The up-regulation of proline synthesis in the meristematic tissues of wheat seedlings upon short-term exposure to osmotic stress.

J Plant Physiol 2019 Apr 4;237:21-29. Epub 2019 Apr 4.

Institute of Botany, University of Natural Resources and Life Sciences, Gregor Mendelstraße 33, 1180 Vienna, Austria. Electronic address:

An increase in the cellular concentration of free proline is a common response of many plants to various types of environmental stress. In this study, we monitored the accumulation of proline and the activities of Δ-pyrroline-5-carboxylate synthetase (P5CS) and Δ-pyrroline-5-carboxylate reductase (P5CR), the key enzymes of proline biosynthesis, in different parts of 4-day-old seedlings of wheat (Triticum aestivum L. cv. Read More

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http://dx.doi.org/10.1016/j.jplph.2019.03.010DOI Listing

Developing biocompatible silver nanoparticles using epigallocatechin gallate for dental use.

Arch Oral Biol 2019 Mar 28;102:106-112. Epub 2019 Mar 28.

Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address:

Objective: To develop silver nanoparticles (AgNPs) using epigallocatechin gallate (EGCG) and evaluate its biocompatibility and inhibition effect on Streptococcus mutans biofilm growth.

Design: AgNPs were synthesized using EGCG as a reducing agent. Cytotoxicity was assessed using half-maximal inhibitory concentration (IC) against human gingival fibroblast (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Read More

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http://dx.doi.org/10.1016/j.archoralbio.2019.03.022DOI Listing

Mechanisms underlying a critical period of respiratory development in the rat.

Respir Physiol Neurobiol 2019 Apr 15. Epub 2019 Apr 15.

Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive, SE, Albuquerque, New Mexico 87108, USA.

Twenty-five years ago, Filiano and Kinney (1994) proposed that a critical period of postnatal development constitutes one of the three risk factors for sudden infant death syndrome (SIDS). The underlying mechanism was poorly understood. In the last 17 years, much has been uncovered on this period in the rat. Read More

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http://dx.doi.org/10.1016/j.resp.2019.04.006DOI Listing

Simultaneous assessment of compound activity on cardiac Nav1.5 peak and late currents in an automated patch clamp platform.

J Pharmacol Toxicol Methods 2019 Apr 15. Epub 2019 Apr 15.

Drug Safety Research and Development, Pfizer Inc., La Jolla, CA 92121, United States of America.

Introduction: High throughput in vitro profiling of the cardiac Nav1.5 peak sodium current (I) is widely used in cardiac safety screening. However, there is no standardized high throughput method to measure late I. Read More

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http://dx.doi.org/10.1016/j.vascn.2019.04.001DOI Listing

Gold nanoparticles loaded with Cullin-5 DNA increase sensitivity to 17-AAG in Cullin-5 Deficient Breast Cancer Cells.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Department of Biological Sciences, Towson University, Towson, MD, United States. Electronic address:

HSP90 inhibitors have the potential to treat many types of cancer due to the dependence of tumor cells on HSP90 for cell growth and proliferation. The Cullin-5 (Cul5) E3 ubiquitin ligase is required for HSP90 inhibitors to induce client protein degradation and subsequent cell death. Cul5 is expressed at low levels in breast cancer cells compared to patient matched controls. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.022DOI Listing

Egg white coated alginate nanoparticles with electron sprayer for potential anticancer application.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen, 518057, PR China; Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, PR China. Electronic address:

To development biomimetic strategy for enhanced cancer therapy, the paclitaxel loaded egg white/sodium alginate nanoparticles were prepared by electronic spray method. Their appearance and particle size were observed by transmission electron microscope and particle size analyzer. The drug release behavior of nanoparticles was determined by high performance liquid chromatography (HPLC). Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.045DOI Listing

Transcriptome signatures of the Pacific white shrimp Litopenaeus vannamei hepatopancreas in response to stress in biofloc culture systems.

Fish Shellfish Immunol 2019 Apr 15. Epub 2019 Apr 15.

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, People's Republic of China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong Province, 266235, China.

Comparative transcriptome analysis via high throughput sequencing was applied to gain knowledge on the immune response in Litopenaeus vannamei reared in biofloc technology systems (BFT). Two types of carbon sources, namely, traditional carbon sources (molasses) and biodegradable polymers [hydroxybutyric acid-co-3-hydroxyvaleric acid (PHBV)] were used in BFT systems. Clear water systems without the addition of carbon sources were treated as the control. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.043DOI Listing
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