5,874,524 results match your criteria Cellular oncology : the official journal of the International Society for Cellular Oncology[Journal]

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Schizandrin A inhibits cellular phenotypes of breast cancer cells by repressing miR-155.

IUBMB Life 2020 Jul 4. Epub 2020 Jul 4.

Department of Breast and Thyroid Surgery, Jining No. 1 People's Hospital, Jining, Shandong, China.

Aims: Schizandrin A (SchA) is a type of lignan with biological properties against oxidation, inflammation, and cancer. Here, we aimed to sustain the bioactive properties of SchA in proliferative and motional phenotypes of MDA-MB-231 cells and their molecular mechanism.

Methods: MDA-MB-231 cells were exposed to SchA. Read More

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http://dx.doi.org/10.1002/iub.2329DOI Listing

Establishment of a Mouse Model for Post-Inflammatory Hyperpigmentation.

Pigment Cell Melanoma Res 2020 Jul 4. Epub 2020 Jul 4.

Department of Dermatology, Yamagata University, Faculty of Medicine, Yamagata, Japan.

Post-inflammatory hyperpigmentation (PIH) is a common cutaneous condition that can cause a disfigured appearance. However, the pathophysiology of PIH remains poorly understood, at least in part, because an appropriate animal model for research has not been established. In order to analyze the pathomechanism of PIH, we successfully induced PIH in a hairless version of transgenic mice (hk14-SCF Tg/HRM) that have a human-type epidermis containing melanin by repeated hapten application of 2,4-dinitrofluorobenzene. Read More

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http://dx.doi.org/10.1111/pcmr.12911DOI Listing

Adhesive pads of gecko and anoline lizards utilize corneous and cytoskeletal proteins for setae development and renewal.

Authors:
Lorenzo Alibardi

J Exp Zool B Mol Dev Evol 2020 Jul 5. Epub 2020 Jul 5.

Comparative Histolab Padova and Department of Biology, University of Bologna, Bologna, Italy.

The formation of the complex pattern of setae in adhesive pads of geckos and anoline lizards has been analyzed by ultrastructural, autoradiographic, and immunohistochemical methods. Setae terminate with spatulated ends responsible for adhesion that allow these lizards to climb vertical substrates and conquer arboreal niches. Setae derive from a complex interfaced molding between two specialized epidermal layers of the shedding complex that determines the cyclical skin molting, Oberhautchen and clear layers. Read More

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http://dx.doi.org/10.1002/jez.b.22976DOI Listing

Facial rejuvenation using stem cell conditioned media combined with skin needling: A split face comparative study.

J Cosmet Dermatol 2020 Jul 5. Epub 2020 Jul 5.

Department of Dermatology, STD's and Andrology, Faculty of Medicine, Minia University, Al Minya, Egypt.

Background: The use of stem cells derived growth factors is representing a novel treatment modality in facial rejuvenation. Nowadays, skin needling is considered a very famous treatment of skin aging. However, the addition of such derived products, augments it's therapeutic efficacy in the management and delay of skin aging. Read More

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http://dx.doi.org/10.1111/jocd.13594DOI Listing

Enhanced local and systemic inflammatory cytokine mRNA expression in women with endometriosis evokes compensatory adaptive regulatory mRNA response that mediates immune suppression and impairs cytotoxicity.

Am J Reprod Immunol 2020 Jul 4:e13298. Epub 2020 Jul 4.

Department of Clinical Microbiology/Infection and Immunology, Umeå University, Umeå, Sweden.

Problem: Endometriosis is a disease characterized by ectopic implantation of endometrium and impaired immune responses. To explore its pathogenic mechanisms, we studied the local and systemic cytokine mRNA profiles and their role in the immunity of patients with endometriosis and healthy controls.

Method Of Study: mRNA for eleven cytokines defining cytotoxic Th1, humoral Th2, regulatory Tr1/Th3 and inflammatory cytokine profiles were characterized locally in endometriotic tissue and endometrium, and systemically in PBMCs from women with endometriosis and healthy controls, using real-time qRT-PCR. Read More

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http://dx.doi.org/10.1111/aji.13298DOI Listing

Establishment of an in vitro 3D model for neuroblastoma enables preclinical investigation of combined tumor-stroma drug targeting.

FASEB J 2020 Jul 5. Epub 2020 Jul 5.

Rheumatology Unit, Department of Medicine, Solna, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.

The majority of anti-cancer therapies target the proliferating tumor cells, while the tumor stroma, principally unaffected, survives, and provide a niche for surviving tumor cells. Combining tumor cell and stroma-targeting therapies thus have a potential to improve patient outcome. The neuroblastoma stroma contains cancer-associated fibroblasts expressing microsomal prostaglandin E synthase-1 (mPGES-1). Read More

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http://dx.doi.org/10.1096/fj.202000684RDOI Listing

Lnc00462717 regulates the permeability of the blood-brain tumor barrier through interaction with PTBP1 to inhibit the miR-186-5p/Occludin signaling pathway.

FASEB J 2020 Jul 5. Epub 2020 Jul 5.

Department of Physiology, Life Science and Biopharmaceutical Institution, Shenyang Pharmaceutical University, Shenyang, P.R. China.

Blood-brain tumor barrier (BTB) severely restricts the efficient delivery of chemotherapeutic drugs into brain tumor tissue, which is a critical obstacle for glioma treatment. Recently, long noncoding RNAs (lncRNAs) have shown as regulation factors of numerous biological processes. In this study, we identified that Lnc00462717 was upregulated in glioma endothelial cells (GECs), and that knockdown of Lnc00462717 significantly increased the BTB permeability. Read More

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http://dx.doi.org/10.1096/fj.202000045RDOI Listing

Biallelic loss-of-function variants in TBC1D2B cause a neurodevelopmental disorder with seizures and gingival overgrowth.

Hum Mutat 2020 Jul 5. Epub 2020 Jul 5.

Institute of Human Genetics, University Medical Center Hamburg-Eppendorf, Hamburg, 20246, Germany.

The family of Tre2-Bub2-Cdc16 (TBC)-domain containing GTPase activating proteins (RABGAPs) are not only known as key regulators of RAB GTPase activity, but also has GAP-independent functions. RAB GTPases are implicated in membrane trafficking pathways, such as vesicular trafficking. We report biallelic loss-of-function variants in TBC1D2B, encoding a member of the TBC/RABGAP family with yet unknown function, as the underlying cause of cognitive impairment, seizures, and/or gingival overgrowth in three individuals from unrelated families. Read More

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http://dx.doi.org/10.1002/humu.24071DOI Listing

Lrrk2 modulation of Wnt signaling during zebrafish development.

J Neurosci Res 2020 Jul 5. Epub 2020 Jul 5.

Department of Neurobiology & Behavior, Stony Brook University, Stony Brook, NY, USA.

Mutations in leucine-rich repeat kinase 2 (lrrk2) are the most common genetic cause of Parkinson's disease. Difficulty in elucidating the pathogenic mechanisms resulting from disease-associated Lrrk2 variants stems from the complexity of Lrrk2 function and activities. Lrrk2 contains multiple protein-protein interacting domains, a GTPase domain, and a kinase domain. Read More

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http://dx.doi.org/10.1002/jnr.24687DOI Listing

IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signaling pathways.

J Oral Rehabil 2020 Jul 5. Epub 2020 Jul 5.

Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.

Background: Dental Stem cell transplantation has become a new method for tooth tissue regeneration. However, its molecular mechanism of the dentinogenic differentiation is still unclear, limited its application. Our previous studies found that Insulin-like growth factor binding protein 5 (IGFBP5) can promote the osteogenic differentiation of periodontal ligament stem cells and the regeneration of periodontal tissues. Read More

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http://dx.doi.org/10.1111/joor.13047DOI Listing

Innate IFN-lambda responses to dsRNA in the human infant airway epithelium and clinical regulatory factors during viral respiratory infections in early life.

Clin Exp Allergy 2020 Jul 5. Epub 2020 Jul 5.

Division of Pediatric Pulmonary and Sleep Medicine. Children's National Medical Center, George Washington University, Washington, D.C, USA.

Introduction: IFN-lambda (type III-IFN-λ1) is a molecule primarily produced by epithelial cells that provides an important first-line defense against viral respiratory infections and has been linked to the pathogenesis of viral induced wheezing in early life. The goal of this study was to better understand the regulation of innate IFN-lambda responses in vitro in primary human infant airway epithelial cells (AECs) and in vivo using nasal aspirates during viral respiratory infections.

Methods: IFN-lambda protein levels were quantified: I) in human infant AECs exposed to (poly(I:C) dsRNA) under different experimental conditions (n=8 donors); and II) in nasal aspirates of young children (≤3 years) hospitalized with viral respiratory infection (n=138) and in uninfected controls (n= 74). Read More

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http://dx.doi.org/10.1111/cea.13701DOI Listing

A case of primary cutaneous B-cell lymphoma with immature features in an old man. Diffuse Large B-cell Lymhpoma with immature features or B-cell Lymhpoblastic Lymphoma?

J Cutan Pathol 2020 Jul 4. Epub 2020 Jul 4.

Oncology and Hematology Unit, Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.

Primary cutaneous B-cell lymphomas are a heterogeneous group of lymphoid neoplasms primarily occurring in the skin. Although most cases are represented by primary cutaneous follicle center cell lymphoma, primary cutaneous marginal zone lymphoma and leg-type diffuse large B-cell lymphoma, other diffuse large B-cell lymphomas and B-cell lymphoblastic lymphoma may rarely present primarily in the skin. In this setting, the presence of histopathologic and immunohistochemical features of cellular immaturity is exceedingly rare and may represent a diagnostic challenge. Read More

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http://dx.doi.org/10.1111/cup.13795DOI Listing

Hemocyte-specific FREP13 abrogates the exogenous bacterial population in the hemolymph and promotes midgut endosymbionts in Anopheles stephensi.

Immunol Cell Biol 2020 Jul 5. Epub 2020 Jul 5.

Laboratory of Host-Parasite Interaction Studies, ICMR-National Institute of Malaria Research, Dwarka, New Delhi, 110077, India.

The mosquito's immune blood cells, 'hemocytes' imparts a highly selective immune response against various micro-organisms/pathogens. Among several immune effectors, FREPs (Fibrinogen related proteins) have been recognized as key modulator of cellular immune responses; however, their physiological relevance has not been investigated in detail. Our ongoing comparative RNA-Seq analysis identified a total of 13 FREPs originating from naïve sugar-fed, blood-fed, bacterial challenged, and Plasmodium vivax-infected hemocytes in the mosquito Anopheles stephensi hemocytes. Read More

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http://dx.doi.org/10.1111/imcb.12374DOI Listing

Brucella abortus-infected platelets modulate activation of neutrophils.

Immunol Cell Biol 2020 Jul 5. Epub 2020 Jul 5.

Instituto de Medicina Experimental (CONICET-Academia Nacional de Medicina), Buenos Aires, Argentina.

Brucellosis is a contagious disease caused by bacteria of the genus Brucella. Platelets have been widely involved in the modulation of the immune response. We have previously reported the modulation of Brucella abortus-mediated infection of monocytes. Read More

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http://dx.doi.org/10.1111/imcb.12373DOI Listing

Ultrastructural investigation of acidocalcisomes and ATPase activity in yeasts Candida guillermondii NP-4 as "complementary" stress-targets.

Lett Appl Microbiol 2020 Jul 4. Epub 2020 Jul 4.

Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, 1 A. Manoogyan Str, 0025, Yerevan, Armenia.

As a result of electron microscopic studies of morphogenesis in yeasts Candida guilliermondii NP-4, the formation of new structures of volutin acidocalcisomes has been established within the cell cytoplasm. Under influence of X-irradiation the changes in morphometric and electron-dense properties of yeast cells were identified: in yeast cytoplasm, the electron-dense volutin granules were increased up to 400 nm in size. After 24 h post-irradiation incubation of yeasts the large volutin pellets are fragmented into smaller number particles in size up to 25-150 nm. Read More

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http://dx.doi.org/10.1111/lam.13348DOI Listing

Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy.

J Sci Food Agric 2020 Jul 5. Epub 2020 Jul 5.

College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, 210095, P.R. China.

Background: Wooden breast (WB) myopathy in broiler chickens is a growing challenge for the poultry industry. Previous multi-omic data have implied that the pathogenesis of WB is associated with the activation of immune system and inflammatory response. However, the intricate mechanisms are not fully understood. Read More

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http://dx.doi.org/10.1002/jsfa.10641DOI Listing

Trichoblastic carcinosarcoma with panfollicular differentiation (panfollicular carcinosarcoma) and CTNNB1 (beta-catenin) mutation.

J Cutan Pathol 2020 Jul 4. Epub 2020 Jul 4.

Department of Pathology, Erasmus University Medical Center, Rotterdam, The Netherlands.

We present a case of trichoblastic carcinosarcoma with panfollicular differentiation. An 80-year-old man presented with a lesion on the left ear which had been present for several months. Histopathology revealed a well-demarcated neoplasm in the dermis composed of intimately intermingled malignant epithelial and mesenchymal cells. Read More

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http://dx.doi.org/10.1111/cup.13794DOI Listing

Monte Carlo dosimetry of a realistic multicellular model of follicular lymphoma in a context of radioimmunotherapy.

Med Phys 2020 Jul 4. Epub 2020 Jul 4.

CRCT, UMR 1037 INSERM, Université Paul Sabatier, F-31037, Toulouse, France.

Purpose: Tumors geometry and radiopharmaceutical biodistribution impact the energy deposition in targeted radionuclide therapy. However, small-scale dosimetry studies generally consider an artificial environment where the tumors are spherical and the radionuclides are homogeneously biodistributed. To bring realism, we developed a dosimetric methodology based on a three-dimensional in vitro model of follicular lymphoma incubated with rituximab, an anti-CD20 monoclonal antibody used in the treatment of non-Hodgkin lymphomas, which might be combined with a radionuclide. Read More

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http://dx.doi.org/10.1002/mp.14370DOI Listing

Generation of functional lungs with pluripotent stem cells.

Allergy 2020 Jul 4. Epub 2020 Jul 4.

Department of Cardiovascular and Thoracic Sciences, Fondazione Policlinico Universitario "A Gemelli" - IRCCS, University of the Sacred Heart. Rome, Italy.

Millions of people of all ages suffer from chronic respiratory diseases (CRDs), including asthma, respiratory allergies and chronic obstructive pulmonary disease. To date, although significant advances have been made in terms of treatment, end-stage CRDs still remains one of the most frequent causes of death worldwide with a death rate that has been increasing over the past few years. Lung transplantation is the only therapeutic option when other treatments fail. Read More

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http://dx.doi.org/10.1111/all.14485DOI Listing

Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models.

Urol J 2020 Jun 23;17(4):413-421. Epub 2020 Jun 23.

Department of Urology, Ufuk University, School of Medicine, Ankara, Turkey.

Purpose: To evaluate the effect of a new mesenchymal stem cell type derived from the neonatal bladder (nMSC-B) on diabetic bladder dysfunction (DBD).

Materials And Methods: nMSC-B were harvested from neonatal male Sprague-Dawley rat's bladder and expanded in culture. nMSC-B were transferred to Type-1 diabetic rats which were induced by a single dose 45 mg/kg Streptozocin (STZ). Read More

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http://dx.doi.org/10.22037/uj.v0i0.5504DOI Listing

The relationship between the presence of HPV infection and biofilm formation in cervicovaginal smears.

Infection 2020 Jul 4. Epub 2020 Jul 4.

Department of Gynecology and Obstetrics, Faculty of Medicine, Hacettepe University, Ankara, Turkey.

Purpose: To demonstrate and understand the association of HPV infection and biofilm formation.

Methods: The study consisted of cervicovaginal samples of 72 women who were evaluated at the colposcopy unit. Papanicolaou staining was used for cytological examination while "Crystal Violet Binding" assay was performed to detect biofilm formation. Read More

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http://dx.doi.org/10.1007/s15010-020-01478-5DOI Listing

Danlou Tablet () Improves Chronic Intermittent Hypoxia-induced Dyslipidemia and Arteriosclerosis by HIF-1 α-Angptl4 mRNA Signaling Pathway.

Chin J Integr Med 2020 Jul 4. Epub 2020 Jul 4.

Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN, 55905, USA.

Objective: To detect whether Danlou Tablet (, DLT) regulates the hypoxia-induced factor (HIF)-1 α-angiopoietin-like 4 (Angptl4) mRNA signaling pathway and explore the role of DLT in treating chronic intermittent hypoxia (CIH)-induced dyslipidemia and arteriosclerosis.

Methods: The mature adipocytes were obtained from 3T3-L1 cell culturation and allocated into 8 groups including control groups (Groups 1 and 5, 0.1 mL of cell culture grade water); DLT groups (Groups 2 and 6, 0. Read More

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http://dx.doi.org/10.1007/s11655-020-3255-8DOI Listing

Role of the bone microenvironment in bone metastasis of malignant tumors - therapeutic implications.

Authors:
Xiaoting Ma Jing Yu

Cell Oncol (Dordr) 2020 Jul 4. Epub 2020 Jul 4.

Cancer Center, Beijing Friendship Hospital, Capital Medical University, No.95 Yong An Road, Xi Cheng District, Beijing, 100050, China.

Background: Bone is one of the most common sites for solid tumor metastasis. Bone metastasis of a malignant tumor seriously affects the quality of life and the overall survival of patients. Evidence has suggested that bone provides a favorable microenvironment that enables disseminated tumor cells to home, proliferate and colonize, leading to the formation of metastases. Read More

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http://dx.doi.org/10.1007/s13402-020-00512-wDOI Listing

Bufalin inhibits hepatitis B virus-associated hepatocellular carcinoma development through androgen receptor dephosphorylation and cell cycle-related kinase degradation.

Cell Oncol (Dordr) 2020 Jul 4. Epub 2020 Jul 4.

Liver Disease Department, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, No.528. Zhangheng Road, Pudong New District, Shanghai, People's Republic of China.

Purpose: Hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC), which has a male predominance, lacks effective therapeutic options. Previously, the cardiac glycoside analogue bufalin has been found to inhibit HBV infection and HCC development. As yet, however, its molecular role in HBV-associated HCC has remained obscure. Read More

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http://dx.doi.org/10.1007/s13402-020-00546-0DOI Listing

Gestational bisphenol A exposure induces fatty liver development in male offspring mice through the inhibition of HNF1b and upregulation of PPARγ.

Cell Biol Toxicol 2020 Jul 4. Epub 2020 Jul 4.

Department of Toxicology, Shaanxi Key Lab of Free Radical Biology and Medicine, the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Air Force Medical University (Fourth Military Medical University), Changle West Road 169, Xi'an, 710032, Shaanxi Province, China.

Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) associated with non-alcoholic fatty liver disease (NAFLD). The effects of gestational BPA exposure on hepatic lipid accumulation in offspring are not fully understood. Here, we investigate the sex-dependent effects of gestational BPA exposure on hepatic lipid and glucose metabolism in the offspring of mice to reveal the mechanisms underlying gestational BPA exposure-associated NAFLD. Read More

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http://dx.doi.org/10.1007/s10565-020-09535-3DOI Listing

Distinct requirements for budding yeast Rev1 and Polη in translesion DNA synthesis across different types of DNA damage.

Authors:
Zihao Wang Wei Xiao

Curr Genet 2020 Jul 4. Epub 2020 Jul 4.

Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, 100048, China.

Certain replication-blocking lesions can escape DNA repair and must be bypassed to prevent fork collapse and cell death. Budding yeast DNA-damage tolerance consists of translesion DNA synthesis (TLS) and template switch. TLS utilizes specialized DNA polymerases to insert nucleotides opposite the damage site, followed by extension, allowing continual replication in the presence of lesions on the template DNA. Read More

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http://dx.doi.org/10.1007/s00294-020-01092-wDOI Listing

Aβ40 Oligomers Promote Survival and Early Neuronal Differentiation of Dentate Gyrus-Isolated Precursor Cells Through Activation of the Akt Signaling Pathway.

Neurotox Res 2020 Jul 4. Epub 2020 Jul 4.

Department of Molecular Biomedicine, Center for Research and Advanced Studies (CINVESTAV-IPN), Av. Instituto Politécnico Nacional 2508, 07360, Mexico City, Mexico.

The amyloid beta-peptide (Aβ) is the low-abundance product of amyloid precursor protein (APP), which is produced lifelong in the healthy brain. The functional properties of Aβ40 and Aβ42 peptides have not been completely elucidated to date. Although, several studies suggest that these peptides have a number of neurotrophic and neurotoxic properties, respectively. Read More

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http://dx.doi.org/10.1007/s12640-020-00253-6DOI Listing

β-Asarone Exerts Antioxidative Effects on HO-Stimulated PC12 Cells by Activating Nrf2/HO-1 Pathway.

Neurochem Res 2020 Jul 4. Epub 2020 Jul 4.

College of Traditional Chinese Medicine·College of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Oxidative stress-mediated neuron damage is considered an important contributor to the pathogenesis and development of neurodegenerative diseases. Although β-asarone is widely known for its neuroprotective pharmacological properties, the exact mechanism of β-asarone against oxidative stress has not been fully elucidated. The aim of the present study was to investigate underlying mechanisms of β-asarone against oxidative damage in PC12 cells. Read More

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http://dx.doi.org/10.1007/s11064-020-03060-9DOI Listing

Effect of Iodine and Selenium on Proliferation, Viability, and Oxidative Stress in HTR-8/SVneo Placental Cells.

Biol Trace Elem Res 2020 Jul 5. Epub 2020 Jul 5.

School of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Adelaide, SA, 5005, Australia.

Adequate maternal micronutrition is vital for placental formation, fetal growth, and development. Oxidative stress adversely affects placental development and function and an association between deficient placental development, oxidative stress, and micronutrient deficiency has been observed. Selenium and iodine are two essential micronutrients with antioxidant properties. Read More

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http://dx.doi.org/10.1007/s12011-020-02277-7DOI Listing

IL-4 and IL-10 promotes phagocytic activity of microglia by up-regulation of TREM2.

Cytotechnology 2020 Jul 4. Epub 2020 Jul 4.

Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China.

Triggering receptor expressed on myeloid cells-2 (TREM2) is an innate immune receptor that promotes phagocytosis by microglia. However, whether TREM2 is related to the stimulus-dependent phagocytic activity of microglia is unclear. In this study, the primary cultured microglia were stimulated with interferon (IFN)-γ, interleukin (IL)-4, and interleukin (IL)-10, respectively, and their phagocytic activity against microbeads and apoptotic neural stem cells (NSCs) was measured. Read More

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http://dx.doi.org/10.1007/s10616-020-00409-4DOI Listing

Molecular link between dietary fibre, gut microbiota and health.

Mol Biol Rep 2020 Jul 4. Epub 2020 Jul 4.

Division of Animal Nutrition, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.

Natural polysaccharides cellulose, hemicelluloses, inulin etc., galactooligosaccharides (GOS), and fructooligosaccharides (FOS) play a significant role in the improvement of gut microbiota balance and human health. These polysaccharides prevent pathogen adhesion that stimulates the immune system and gut barrier function by servicing as fermentable substrates for the gut microbiota. Read More

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http://dx.doi.org/10.1007/s11033-020-05611-3DOI Listing

Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay.

Mol Biol Rep 2020 Jul 4. Epub 2020 Jul 4.

Department of Medical Biology, Faculty of Medicine, Cukurova University, Adana, Turkey.

Antimicrobial irrigation solutions are widely used under clinical settings. Their effect on dental tissue is a subject of recent research, which aims for a safer irrigant for clinical use. In this regard, here our goal was to evaluate the cytotoxicity and the genotoxicity of calcium hypochlorite (Ca(OCl)) solution, along with NaOCl, on Mouse embryonic fibroblast cells (NIH3T3). Read More

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http://dx.doi.org/10.1007/s11033-020-05624-yDOI Listing

Secondary metabolites from acridocarpus orientalis inhibits 4T1 cells and promotes mesenchymal stem cells (MSCs) proliferation.

Mol Biol Rep 2020 Jul 4. Epub 2020 Jul 4.

Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, P. O. Box: 33, Nizwa, PC, 616, Oman.

Among medicinal plants, Acridocarpus orientalis (AO) possesses a remarkable anti-cancer potential, possibly because of its anti-oxidant property. In this study, the leaf and stem extracts from AO were assessed to find the bioactive compound with selective anti-cancer properties. The MTT viability and live and dead assays revealed that around 80% and 98% of 4T1 cells survival were declined after 48 h incubation with leaf and stem extracts, respectively. Read More

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http://dx.doi.org/10.1007/s11033-020-05632-yDOI Listing

Gut microbiota and undigested food constituents modify toxin composition and suppress the genotoxicity of a naturally occurring mixture of Alternaria toxins in vitro.

Arch Toxicol 2020 Jul 4. Epub 2020 Jul 4.

Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Währinger Str. 42, 1090, Vienna, Austria.

Molds of the genus Alternaria produce several mycotoxins, some of which may pose a threat for health due to their genotoxicity. Due to the lack of adequate toxicological and occurrence data, they are currently not regulated. Interactions between mycotoxins, gut microbiota and food constituents might occur after food ingestion, modifying the bioavailability and, therefore, overall toxicity of mycotoxins. Read More

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http://dx.doi.org/10.1007/s00204-020-02831-1DOI Listing

Valproic acid promotes mitochondrial dysfunction in primary human hepatocytes in vitro; impact of C/EBPα-controlled gene expression.

Arch Toxicol 2020 Jul 4. Epub 2020 Jul 4.

Maastricht University, Maastricht, The Netherlands.

Valproic acid (VPA) is a frequently prescribed anti-epileptic drug which is known to cause liver toxicity and steatosis through mitochondrial dysfunction. Nevertheless the mechanisms underlying these adverse effects are incompletely understood. In this study, we determined the effect of relatively short (3 h) or prolonged (72 h) exposure to VPA on mitochondrial function in primary human hepatocytes (PHHs). Read More

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http://dx.doi.org/10.1007/s00204-020-02835-xDOI Listing

Photodynamic treatment with purpurin 18 effectively inhibits triple negative breast cancer by inducing cell apoptosis.

Lasers Med Sci 2020 Jul 5. Epub 2020 Jul 5.

School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.

This study aims to evaluate the photodynamic efficacy of purpurin 18 (pu-18) on triple negative breast cancer both in vitro and in vivo. Two states of 4T1 cells, 2D culture and 3D spheroids, were used to evaluate the photodynamic action of pu-18 in vitro. The in vitro study results indicated that for the 4T1 2D cell culture, the photodynamic therapy (PDT) treatment showed significant photocytotoxicity at low pu-18 concentrations following light irradiation. Read More

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http://dx.doi.org/10.1007/s10103-020-03035-wDOI Listing

The cadmium tolerance development of poplar callus is influenced by silicon.

Ecotoxicology 2020 Jul 4. Epub 2020 Jul 4.

Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia.

Cadmium-tolerant plants were studied for their possible usage in phytoremediation techniques. However, their response to cadmium cations at a cellular level has not been properly studied. Silicon is a beneficial element that seems to change the plant's response to the Cd presence. Read More

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http://dx.doi.org/10.1007/s10646-020-02242-zDOI Listing

Dipeptidyl Peptidase-4 Inhibitor Decreases Allograft Vasculopathy Via Regulating the Functions of Endothelial Progenitor Cells in Normoglycemic Rats.

Cardiovasc Drugs Ther 2020 Jul 4. Epub 2020 Jul 4.

Division of Cardiovascular Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.

Purpose: Chronic rejection induces the occurrence of orthotopic allograft transplantation (OAT) vasculopathy, which results in failure of the donor organ. Numerous studies have demonstrated that in addition to regulating blood sugar homeostasis, dipeptidyl peptidase-4 (DPP-4) inhibitors can also provide efficacious therapeutic and protective effects against cardiovascular diseases. However, their effects on OAT-induced vasculopathy remain unknown. Read More

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http://dx.doi.org/10.1007/s10557-020-07013-wDOI Listing

RP11-323N12.5 promotes the malignancy and immunosuppression of human gastric cancer by increasing YAP1 transcription.

Gastric Cancer 2020 Jul 4. Epub 2020 Jul 4.

Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210093, Jiangsu, People's Republic of China.

Background: YAP1 is a core protein of the Hippo signaling pathway and is associated with malignancy and immunosuppression. In the present study, we discovered a novel lncRNA, RP11-323N12.5, with tumor promotion and immunosuppression activities through enhancing transcription of YAP1. Read More

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http://dx.doi.org/10.1007/s10120-020-01099-9DOI Listing

Gamma linolenic acid suppresses hypoxia-induced proliferation and invasion of non-small cell lung cancer cells by inhibition of HIF1α.

Genes Genomics 2020 Jul 4. Epub 2020 Jul 4.

Department of Nutrition, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.

Background: The main therapies for cancer often results in many side effects and drug resistance. Gamma linolenic acid (GLA) is a kind of natural reagent with negligible cytotoxicity.

Objective: This work aims at detecting whether GLA possesses anti-cancer activity in NSCLC cells and elucidating the potential molecular mechanism. Read More

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http://dx.doi.org/10.1007/s13258-020-00961-5DOI Listing

Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome - a diagnostic in vitro tissue remodeling platform.

J Exp Orthop 2020 Jul 4;7(1):48. Epub 2020 Jul 4.

Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, Building 15, Groene Loper, Gemini-Zuid 4.12, PO Box 513, 5600MB, Eindhoven, The Netherlands.

Purpose: Upon anterior cruciate ligament (ACL) rupture, reconstruction is often required, with the hamstring tendon autograft as most widely used treatment. Post-operative autograft remodeling enhances graft rupture risk, which occurs in up to 10% of the patient population, increasing up to 30% of patients aged under 20 years. Therefore, this research aimed to identify potential biological predictors for graft rupture, derived from patient-specific tissue remodeling-related cell properties in an in vitro micro-tissue platform. Read More

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http://dx.doi.org/10.1186/s40634-020-00266-2DOI Listing

Extrahepatic metabolism of ibrutinib.

Invest New Drugs 2020 Jul 4. Epub 2020 Jul 4.

Division of Pharmacoepidemiology & Clinical Pharmacology, Faculty of Science, Department of Pharmaceutical Sciences, Utrecht University, Universiteitsweg 99, 3584 CG, Utrecht, The Netherlands.

Ibrutinib is a first-in-class Bruton's kinase inhibitor used in the treatment of multiple lymphomas. In addition to CYP3A4-mediated metabolism, glutathione conjugation can be observed. Subsequently, metabolism of the conjugates and finally their excretion in feces and urine occurs. Read More

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http://dx.doi.org/10.1007/s10637-020-00970-xDOI Listing

Rational design of genetically encoded reporter genes for optical imaging of apoptosis.

Apoptosis 2020 Jul 4. Epub 2020 Jul 4.

Institute of Medical Research, Northwestern Polytechnical University, Xi'an, 710072, China.

Apoptosis is a process in which cells are genetically regulated to cause a series of changes in morphology and metabolic activity, which ultimately lead to cell death. Apoptosis plays a vital role in the entire life cycle of an organism. Too much or too little apoptosis can cause a variety of diseases. Read More

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http://dx.doi.org/10.1007/s10495-020-01621-5DOI Listing

Expression of ACE2 in Human Neurons Supports the Neuro-Invasive Potential of COVID-19 Virus.

Cell Mol Neurobiol 2020 Jul 4. Epub 2020 Jul 4.

Department of Pharmacology & Experimental Therapeutics, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA, 70112, USA.

The recent outbreak of 2019 coronavirus disease (COVID-19), caused by a novel coronavirus, has now spread quickly worldwide. Like the severe acute respiratory syndrome coronavirus (SARS-CoV), this novel type of coronavirus, SARS-CoV-2, has been demonstrated to utilize angiotensin-converting enzyme 2 (ACE2) as an entry point to the cells. There is a growing body of reports indicating that COVID-19 patients, especially those in severe condition, exhibit neurological symptoms, thus supporting the possibility that SARS-CoV-2 could infect and damage neurons within the central nervous system in humans. Read More

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http://dx.doi.org/10.1007/s10571-020-00915-1DOI Listing

Agent-based modelling and parameter sensitivity analysis with a finite-element method for skin contraction.

Biomech Model Mechanobiol 2020 Jul 4. Epub 2020 Jul 4.

Delft Institute of Applied Mathematics, Delft University of Technology, Van Mourik Broekmanweg 6, 2628 XE, Delft, The Netherlands.

In this paper, we extend the model of wound healing by Boon et al. (J Biomech 49(8):1388-1401, 2016). In addition to explaining the model explicitly regarding every component, namely cells, signalling molecules and tissue bundles, we categorized fibroblasts as regular fibroblasts and myofibroblasts. Read More

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http://dx.doi.org/10.1007/s10237-020-01354-zDOI Listing

Titanium particles damage osteocytes and inhibit osteoblast differentiation.

J Exp Orthop 2020 Jul 4;7(1):47. Epub 2020 Jul 4.

Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, China.

Purposes: to study the effect of titanium particles on MLO-Y4 and the effects of osteocytes alterations on osteoblasts.

Methods: cultured MLO-Y4 osteocytes were exposed to different concentrations of titanium (Ti) particles, cell viability was measured using the Cell Counting Kit-8 (CCK-8) assay, apoptosis of MLO-Y4 cells was evaluated by flow cytometry, Real-time PCR quantification of mRNA expression of SOST, at the same time with Western Blot detection sclerosteosis protein expression levels.MC3T3-E1 cells culture with MLO-Y4 cells exposed to different concentrations of titanium (Ti) particles in vitro, in order to detection of osteoblast osteogenetic activity. Read More

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http://dx.doi.org/10.1186/s40634-020-00268-0DOI Listing

Towards reconstructing the dipteran demise of an ancient essential gene: E3 ubiquitin ligase Murinedouble minute.

Dev Genes Evol 2020 Jul 4. Epub 2020 Jul 4.

Department of Biological Sciences, Wayne State University, 5047 Gullen Mall, Detroit, MI, 48202, USA.

Genome studies have uncovered many examples of essential gene loss, raising the question of how ancient genes transition from essentiality to dispensability. We explored this process for the deeply conserved E3 ubiquitin ligase Murine double minute (Mdm), which is lacking in Drosophila despite the conservation of its main regulatory target, the cellular stress response gene p53. Conducting gene expression and knockdown experiments in the red flour beetle Tribolium castaneum, we found evidence that Mdm has remained essential in insects where it is present. Read More

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http://dx.doi.org/10.1007/s00427-020-00663-8DOI Listing

Complete Genome Sequence and Carbohydrates-Active EnZymes (CAZymes) Analysis of Lactobacillus paracasei DTA72, a Potential Probiotic Strain with Strong Capability to Use Inulin.

Curr Microbiol 2020 Jul 4. Epub 2020 Jul 4.

Department of Agronomy Food Natural Resources Animal and Environment (DAFNAE), University of Padova, viale dell'Università 16, 35020, Legnaro, PD, Italy.

The whole genome sequence of Lactobacillus paracasei DTA72, isolated from healthy infant feces, is reported, along with the Carbohydrates-Active enZymes (CAZymes) analysis and an in silico safety assessment. Strain DTA72 had previously demonstrated some interesting potential probiotic features, such as a good resistance to gastrointestinal conditions and an anti-Listeria activity. The 3. Read More

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http://dx.doi.org/10.1007/s00284-020-02089-xDOI Listing

Protein structure analysis of the interactions between SARS-CoV-2 spike protein and the human ACE2 receptor: from conformational changes to novel neutralizing antibodies.

Cell Mol Life Sci 2020 Jul 4. Epub 2020 Jul 4.

Laboratory of Biochemistry, Molecular and Structural Biology, Department of Biosciences, Biotechnologies, Biopharmaceutics, University of Bari, Via E. Orabona, 4, 70125, Bari, Italy.

The recent severe acute respiratory syndrome, known as Coronavirus Disease 2019 (COVID-19) has spread so much rapidly and severely to induce World Health Organization (WHO) to declare a state of emergency over the new coronavirus SARS-CoV-2 pandemic. While several countries have chosen the almost complete lock-down for slowing down SARS-CoV-2 spread, the scientific community is called to respond to the devastating outbreak by identifying new tools for diagnosis and treatment of the dangerous COVID-19. With this aim, we performed an in silico comparative modeling analysis, which allows gaining new insights into the main conformational changes occurring in the SARS-CoV-2 spike protein, at the level of the receptor-binding domain (RBD), along interactions with human cells angiotensin-converting enzyme 2 (ACE2) receptor, that favor human cell invasion. Read More

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http://dx.doi.org/10.1007/s00018-020-03580-1DOI Listing

Differential immune microenvironmental features of microsatellite-unstable colorectal cancers according to Fusobacterium nucleatum status.

Cancer Immunol Immunother 2020 Jul 4. Epub 2020 Jul 4.

Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.

It has been suggested that Fusobacterium nucleatum (Fn) may differentially impact tumor immune responses according to microsatellite instability (MSI) status in colorectal cancers (CRCs). We aimed to reveal the detailed relationship between intratumoral Fn and immune microenvironmental features in MSI-high CRCs. A total of 126 MSI-high CRCs were subjected to analyses for intratumoral Fn DNA load using quantitative PCR and for densities of tumor-infiltrating immune cells, including CD3 T cells, CD4 T cells, CD8 T cells, FoxP3 T cells, CD68 macrophages, CD163 macrophages, and CD177 neutrophils, at invasive margin (IM) and center of tumor (CT) areas using computational image analysis of immunohistochemistry. Read More

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http://dx.doi.org/10.1007/s00262-020-02657-xDOI Listing
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