2,613 results match your criteria Cellular microbiology[Journal]


α-haemolysin production, as a single factor, causes fulminant sepsis in a model of Escherichia coli induced bacteraemia.

Cell Microbiol 2019 Feb 13:e13017. Epub 2019 Feb 13.

Department of Biomedicine, Aarhus University, Aarhus C, Denmark.

α-haemolysin (HlyA) from uropathogenic Escherichia coli has been demonstrated to be a significant virulence factor for ascending urinary tract infections. Once the E. coli reach the well-vascularised kidneys, there is a high risk of bacteraemia and a subsequent septic host response. Read More

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http://dx.doi.org/10.1111/cmi.13017DOI Listing
February 2019

Integrity under Stress: Host Membrane Remodeling and Damage by Fungal Pathogens.

Cell Microbiol 2019 Feb 10:e13016. Epub 2019 Feb 10.

Keenan Research Centre for Biomedical Sciences, St. Michael's Hospital, Toronto, Canada.

Membrane bilayers of eukaryotic cells are an amalgam of lipids and proteins that distinguish organelles and compartmentalize cellular functions. The mammalian cell has evolved mechanisms to sense membrane tension or damage and respond as needed. In the case of the plasma membrane and phagosomal membrane these bilayers act as a barrier to microorganisms and are a conduit by which the host interacts with pathogens, including fungi such as Candida, Cryptococcus, Aspergillus or Histoplasma species. Read More

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http://dx.doi.org/10.1111/cmi.13016DOI Listing
February 2019

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Cell Microbiol 2019 Jan 31:e13014. Epub 2019 Jan 31.

School of life sciences, Sun Yat-sen University, Guangzhou, China.

The major virulence determinant of Legionella pneumophila is the type IVB secretion system (T4BSS), which delivers approximately 330 effector proteins into the host cell to modulate various cellular processes. However, the functions of most effector proteins remain unclear. WipA, an effector, was the first phosphotyrosine phosphatase of Legionella with unknown function. Read More

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http://dx.doi.org/10.1111/cmi.13014DOI Listing
January 2019
1 Read

Mycoplasma pneumoniae protects infected epithelial cells from hydrogen peroxide-induced cell detachment.

Cell Microbiol 2019 Jan 31:e13015. Epub 2019 Jan 31.

Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, Kurume, Japan.

Epithelial cell shedding is a defense mechanism against infectious microbes that use these cells as an infection foothold, and that eliminates microbes from infection foci by removing infected cells. Mycoplasma pneumoniae, a causative agent of respiratory infections, is known to adhere to and colonize the surface of ciliated airway epithelial cells; it produces a large amount of hydrogen peroxide, indicating its capability of regulating hydrogen peroxide-induced infected cell detachment. In this study, we found that M. Read More

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http://dx.doi.org/10.1111/cmi.13015DOI Listing
January 2019
4.915 Impact Factor

Dysregulated haemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish.

Cell Microbiol 2019 Jan 31:e13010. Epub 2019 Jan 31.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

Inflammasomes are important innate immune components in mammals. However, the bacterial factors modulating inflammasome activation in fish, and the mechanisms by which they alter fish immune defences, remain to be investigated. In this work, a mutant of the fish pathogen Edwardsiella piscicida (E. Read More

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http://dx.doi.org/10.1111/cmi.13010DOI Listing
January 2019

SH3-class Ras guanine nucleotide exchange factors are essential for Aspergillus fumigatus invasive growth.

Cell Microbiol 2019 Jan 30:e13013. Epub 2019 Jan 30.

Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Proper hyphal morphogenesis is essential for the establishment and progression of invasive disease caused by filamentous fungi. In the human pathogen Aspergillus fumigatus, signalling cascades driven by Ras and Ras-like proteins orchestrate a wide variety of cellular processes required for hyphal growth. For activation, these proteins require interactions with Ras-subfamily-specific guanine nucleotide exchange factors (RasGEFs). Read More

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http://dx.doi.org/10.1111/cmi.13013DOI Listing
January 2019
1 Read

Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli.

Cell Microbiol 2019 Jan 23:e13012. Epub 2019 Jan 23.

Norwich Medical School, University of East Anglia, Norwich, UK.

Enteroaggregative Escherichia coli (EAEC) are important intestinal pathogens causing acute and persistent diarrhoeal illness worldwide. Although many putative EAEC virulence factors have been identified, their association with pathogenesis remains unclear. As environmental cues can modulate bacterial virulence, we investigated the effect of oxygen and human intestinal epithelium on EAEC virulence gene expression to determine the involvement of respective gene products in intestinal colonisation and pathogenesis. Read More

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http://dx.doi.org/10.1111/cmi.13012DOI Listing
January 2019

Calcium negatively regulates secretion from dense granules in Toxoplasma gondii.

Cell Microbiol 2019 Jan 23:e13011. Epub 2019 Jan 23.

Department of Biochemistry, University of Cambridge, Cambridge, UK.

Apicomplexan parasites including Toxoplasma gondii and Plasmodium spp. manufacture a complex arsenal of secreted proteins used to interact with and manipulate their host environment. These proteins are organised into three principle exocytotic compartment types according to their functions: micronemes for extracellular attachment and motility, rhoptries for host cell penetration, and dense granules for subsequent manipulation of the host intracellular environment. Read More

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http://doi.wiley.com/10.1111/cmi.13011
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http://dx.doi.org/10.1111/cmi.13011DOI Listing
January 2019
2 Reads

Distinct Mycobacterium marinum phosphatases determine pathogen vacuole phosphoinositide pattern, phagosome maturation, and escape to the cytosol.

Cell Microbiol 2019 Jan 17:e13008. Epub 2019 Jan 17.

Faculty of Medicine, Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.

The causative agent of tuberculosis, Mycobacterium tuberculosis, and its close relative Mycobacterium marinum manipulate phagocytic host cells, thereby creating a replication-permissive compartment termed the Mycobacterium-containing vacuole (MCV). The phosphoinositide (PI) lipid pattern is a crucial determinant of MCV formation and is targeted by mycobacterial PI phosphatases. In this study, we establish an efficient phage transduction protocol to construct defined M. Read More

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http://doi.wiley.com/10.1111/cmi.13008
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http://dx.doi.org/10.1111/cmi.13008DOI Listing
January 2019
6 Reads

Illuminating how malaria parasites export proteins into host erythrocytes.

Cell Microbiol 2019 Jan 17:e13009. Epub 2019 Jan 17.

School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia.

Plasmodium parasites that cause the disease malaria have developed an elaborate trafficking pathway to facilitate the export of hundreds of effector proteins into their host cell, the erythrocyte. In this review, we outline how certain effector proteins contribute to parasite survival, virulence, and immune evasion. We also highlight how parasite proteins destined for export are recognised at the endoplasmic reticulum to facilitate entry into the export pathway and how the effector proteins are able to transverse the bounding parasitophorous vaculoar membrane via the Plasmodium translocon of exported proteins to gain access to the host cell. Read More

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http://dx.doi.org/10.1111/cmi.13009DOI Listing
January 2019
1 Read

Cellular microbiology interview-Dr. Jason King.

Authors:
Jason S King

Cell Microbiol 2019 Jan 16:e13007. Epub 2019 Jan 16.

Royal Society University Research Fellow, Department of Biomedical Science, The University of Sheffield, Sheffield, UK.

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http://dx.doi.org/10.1111/cmi.13007DOI Listing
January 2019
1 Read

miR-193b represses influenza A virus infection by inhibiting Wnt/β-catenin signalling.

Cell Microbiol 2019 Jan 16:e13001. Epub 2019 Jan 16.

Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.

Due to an increasing emergence of new and drug-resistant strains of the influenza A virus (IAV), developing novel measures to combat influenza is necessary. We have previously shown that inhibiting Wnt/β-catenin pathway reduces IAV infection. In this study, we aimed to identify antiviral human microRNAs (miRNAs) that target the Wnt/β-catenin signalling pathway. Read More

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http://doi.wiley.com/10.1111/cmi.13001
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http://dx.doi.org/10.1111/cmi.13001DOI Listing
January 2019
8 Reads

The lipoprotein HP1454 of Helicobacter pylori regulates T-cell response by shaping T-cell receptor signalling.

Cell Microbiol 2019 Jan 15:e13006. Epub 2019 Jan 15.

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Helicobacter pylori (HP) is a Gram-negative bacterium that chronically infects the stomach of more than 50% of human population and represents a major cause of gastric cancer, gastric lymphoma, gastric autoimmunity, and peptic ulcer. It still remains to be elucidated, which HP virulence factors are important in the development of gastric disorders. Here, we analysed the role of the HP protein HP1454 in the host-pathogen interaction. Read More

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http://dx.doi.org/10.1111/cmi.13006DOI Listing
January 2019
6 Reads
4.915 Impact Factor

Role of distinct type-IV-secretion systems and secreted effector sets in host adaptation by pathogenic Bartonella species.

Cell Microbiol 2019 Mar 6;21(3):e13004. Epub 2019 Feb 6.

Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland.

The α-proteobacterial genus Bartonella comprises a large number of facultative intracellular pathogens that share a common lifestyle hallmarked by hemotrophic infection and arthropod transmission. Speciation in the four deep-branching lineages (L1-L4) occurred by host adaptation facilitating the establishment of long lasting bacteraemia in specific mammalian reservoir host(s). Two distinct type-IV-secretion systems (T4SSs) acquired horizontally by different Bartonella lineages mediate essential host interactions during infection and represent key innovations for host adaptation. Read More

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http://dx.doi.org/10.1111/cmi.13004DOI Listing
March 2019
1 Read

Multimodal analysis of Plasmodium knowlesi-infected erythrocytes reveals large invaginations, swelling of the host cell, and rheological defects.

Cell Microbiol 2019 Jan 11:e13005. Epub 2019 Jan 11.

Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, Melbourne, Victoria, Australia.

The simian parasite Plasmodium knowlesi causes severe and fatal malaria infections in humans, but the process of host cell remodelling that underpins the pathology of this zoonotic parasite is only poorly understood. We have used serial block-face scanning electron microscopy to explore the topography of P. knowlesi-infected red blood cells (RBCs) at different stages of asexual development. Read More

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http://dx.doi.org/10.1111/cmi.13005DOI Listing
January 2019
2 Reads

Cellular microbiology interview-Dr. Melanie Blokesch.

Authors:
Melanie Blokesch

Cell Microbiol 2019 Jan 4:e13002. Epub 2019 Jan 4.

Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

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http://dx.doi.org/10.1111/cmi.13002DOI Listing
January 2019
1 Read

Host cell protein LAMP-2 is the receptor for Trypanosoma cruzi surface molecule gp82 that mediates invasion.

Cell Microbiol 2019 Jan 4:e13003. Epub 2019 Jan 4.

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

Host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is mediated by MT-specific surface molecule gp82, which binds to a still unidentified receptor, inducing lysosome spreading and exocytosis required for the parasitophorous vacuole formation. We examined the involvement of the major lysosome membrane-associated LAMP proteins in MT invasion. First, human epithelial HeLa cells were incubated with MT in the presence of antibody to LAMP-1 or LAMP-2. Read More

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http://dx.doi.org/10.1111/cmi.13003DOI Listing
January 2019
1 Read
4.915 Impact Factor

Transmission of the malaria parasite requires ferlin for gamete egress from the red blood cell.

Cell Microbiol 2018 Dec 29:e12999. Epub 2018 Dec 29.

Center for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, Heidelberg, Germany.

Ferlins mediate calcium-dependent vesicular fusion. Although conserved throughout eukaryotic evolution, their function in unicellular organisms including apicomplexan parasites is largely unknown. Here, we define a crucial role for a ferlin-like protein (FLP) in host-to-vector transmission of the rodent malaria parasite Plasmodium berghei. Read More

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http://doi.wiley.com/10.1111/cmi.12999
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http://dx.doi.org/10.1111/cmi.12999DOI Listing
December 2018
3 Reads

Targeting host lipid flows: Exploring new antiviral and antibiotic strategies.

Cell Microbiol 2019 Mar 17;21(3):e12996. Epub 2019 Jan 17.

Cell Structure Lab, National Centre for Biotechnology, CNB-CSIC, Madrid, Spain.

Bacteria and viruses pose serious challenges for humans because they evolve continuously. Despite ongoing efforts, antiviral drugs to treat many of the most troubling viruses have not been approved yet. The recent launch of new antimicrobials is generating hope as more and more pathogens around the world become resistant to available drugs. Read More

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http://dx.doi.org/10.1111/cmi.12996DOI Listing
March 2019
1 Read

The structure of the Shiga toxin 2a A-subunit dictates the interactions of the toxin with blood components.

Cell Microbiol 2018 Dec 22:e13000. Epub 2018 Dec 22.

Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.

Hemolytic uremic syndrome (eHUS) is a severe complication of human infections with Shiga toxins (Stxs)-producing Escherichia coli. A key step in the pathogenesis of eHUS is the interaction of Stxs with blood components before the targeting of renal endothelial cells. Here, we show that a single proteolytic cleavage in the Stx2a A-subunit, resulting into two fragments (A1 and A2) linked by a disulfide bridge (cleaved Stx2a), dictates different binding abilities. Read More

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http://doi.wiley.com/10.1111/cmi.13000
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http://dx.doi.org/10.1111/cmi.13000DOI Listing
December 2018
6 Reads

Discordant susceptibility of inbred C57BL/6 versus outbred CD1 mice to experimental fungal sepsis.

Cell Microbiol 2018 Dec 21:e12995. Epub 2018 Dec 21.

Immunoreceptors of the Innate and Adaptive System team, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.

Individual susceptibility differences to fungal infection following invasive and/or immunosuppressive medical interventions are an important clinical issue. In order to explore immune response-related factors that may be linked to fungal infection susceptibility, we have compared the response of inbred C57BL/6J and outbred CD1 mouse strains to different experimental models of fungal sepsis. The challenge of animals with the zymosan-induced generalised inflammation model revealed poorer survival rates in C57BL/6J, consistent with lower Th1 cytokine interferon (IFN)-γ serum levels, compared with CD1 mice. Read More

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http://dx.doi.org/10.1111/cmi.12995DOI Listing
December 2018
3 Reads

In or out: Phagosomal escape of Staphylococcus aureus.

Cell Microbiol 2019 Mar 18;21(3):e12997. Epub 2019 Jan 18.

Chair of Microbiology, University of Würzburg, Würzburg, Germany.

Staphylococcus aureus is internalised by host cells in vivo, and recent research results suggest that the bacteria use this intracellularity to persist in the host and form a reservoir for recurrent infections. However, in different cells types, the pathogen resorts to alternative strategies to survive phagocytosis and the antimicrobial mechanisms of host cells. In non-professional phagocytes, S. Read More

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http://dx.doi.org/10.1111/cmi.12997DOI Listing
March 2019
1 Read

Blood treatment of Lyme borreliae demonstrates the mechanism of CspZ-mediated complement evasion to promote systemic infection in vertebrate hosts.

Cell Microbiol 2019 Feb 7;21(2):e12998. Epub 2019 Jan 7.

Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY, USA.

Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most common vector-borne disease in the United States and Europe. The spirochetes are transmitted from mammalian and avian reservoir hosts to humans via ticks. Following tick bites, spirochetes colonize the host skin and then disseminate haematogenously to various organs, a process that requires this pathogen to evade host complement, an innate immune defence system. Read More

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http://doi.wiley.com/10.1111/cmi.12998
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http://dx.doi.org/10.1111/cmi.12998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336514PMC
February 2019
9 Reads

Cellular microbiology interview-Dr Marek Basler.

Authors:
Marek Basler

Cell Microbiol 2018 Dec 16:e12991. Epub 2018 Dec 16.

Biozentrum, Universitat Basel, Switzerland.

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http://dx.doi.org/10.1111/cmi.12991DOI Listing
December 2018
1 Read

The puzzling construction of the conidial outer layer of Aspergillus fumigatus.

Cell Microbiol 2018 Dec 15:e12994. Epub 2018 Dec 15.

Aspergillus Unit, Institut Pasteur, Paris, France.

If the mycelium of Aspergillus fumigatus is very short-lived in the laboratory, conidia can survive for years. This survival capacity and extreme resistance to environmental insults is a major biological characteristic of this fungal species. Moreover, conidia, which easily reach the host alveola, are the infective propagules. Read More

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http://dx.doi.org/10.1111/cmi.12994DOI Listing
December 2018
11 Reads

Infection of HeLa cells with Chlamydia trachomatis inhibits protein synthesis and causes multiple changes to host cell pathways.

Cell Microbiol 2018 Dec 14:e12993. Epub 2018 Dec 14.

Institute for Microbiology and Hygiene, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

The obligate intracellular bacterium Chlamydia trachomatis replicates in a cytosolic vacuole in human epithelial cells. Infection of human cells with C. trachomatis causes substantial changes to many host cell-signalling pathways, but the molecular basis of such influence is not well understood. Read More

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http://dx.doi.org/10.1111/cmi.12993DOI Listing
December 2018
4 Reads

Interferon-gamma production in Lyme arthritis synovial tissue promotes differentiation of fibroblast-like synoviocytes into immune effector cells.

Cell Microbiol 2019 Feb 4;21(2):e12992. Epub 2019 Jan 4.

Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.

Lyme arthritis (LA), a late disease manifestation of Borrelia burgdorferi infection, usually resolves with antibiotic therapy. However, some patients develop proliferative synovitis lasting months to several years after spirochetal killing, called postinfectious LA. In this study, we phenotyped haematopoietic and stromal cell populations in the synovial lesion ex vivo and used these findings to generate an in vitro model of LA using patient-derived fibroblast-like synoviocytes (FLS). Read More

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http://dx.doi.org/10.1111/cmi.12992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336510PMC
February 2019
4 Reads

Leptospira species promote a pro-inflammatory phenotype in human neutrophils.

Cell Microbiol 2019 Feb 21;21(2):e12990. Epub 2018 Dec 21.

Laboratory of Animal Viruses, Institute of Biotechnology and Molecular Biology, CONICET-UNLP, La Plata, Argentina.

Leptospirosis is a global zoonosis caused by pathogenic Leptospira. Neutrophils are key cells against bacterial pathogens but can also contribute to tissue damage. Because the information regarding the role of human neutrophils in leptospirosis is scant, we comparatively analysed the human neutrophil's response to saprophytic Leptospira biflexa serovar Patoc (Patoc) and the pathogenic Leptospira interrogans serovar Copenhageni (LIC). Read More

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http://dx.doi.org/10.1111/cmi.12990DOI Listing
February 2019
3 Reads

Acetylation is the most abundant actin modification in Entamoeba histolytica and modifications of actin's amino-terminal domain change cytoskeleton activities.

Cell Microbiol 2018 Dec 2:e12983. Epub 2018 Dec 2.

Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France.

Actin is one of the most conserved, abundant, and ubiquitous proteins in all eukaryotes characterised to date. Posttranslation modifications of actin modify the organisation of the actin-rich cytoskeleton. In particular, chemical modifications of actin's amino-terminal region determine how filamentous actin is organised into scaffolds. Read More

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http://dx.doi.org/10.1111/cmi.12983DOI Listing
December 2018
2 Reads

The Class IV human deacetylase, HDAC11, exhibits anti-influenza A virus properties via its involvement in host innate antiviral response.

Cell Microbiol 2018 Dec 4:e12989. Epub 2018 Dec 4.

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.

Histone deacetylase 11 (HDAC11) is most recently discovered deacetylase. Here, we demonstrate that human HDAC11 exhibits anti-influenza A virus (IAV) properties. We found that knockdown of HDAC11 expression augments IAV growth kinetics in human lung epithelial cells A549 by up to 1 log. Read More

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http://doi.wiley.com/10.1111/cmi.12989
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http://dx.doi.org/10.1111/cmi.12989DOI Listing
December 2018
9 Reads

Listeriolysin O: A phagosome-specific cytolysin revisited.

Cell Microbiol 2019 Mar 15;21(3):e12988. Epub 2019 Jan 15.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California.

Listeriolysin O (LLO) is an essential determinant of Listeria monocytogenes pathogenesis that mediates the escape of L. monocytogenes from host cell vacuoles, thereby allowing replication in the cytosol without causing appreciable cell death. As a member of the cholesterol-dependent cytolysin (CDC) family of pore-forming toxins, LLO is unique in that it is secreted by a facultative intracellular pathogen, whereas all other CDCs are produced by pathogens that are largely extracellular. Read More

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http://doi.wiley.com/10.1111/cmi.12988
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http://dx.doi.org/10.1111/cmi.12988DOI Listing
March 2019
9 Reads

The relapsing fever spirochete Borrelia turicatae persists in the highly oxidative environment of its soft-bodied tick vector.

Cell Microbiol 2019 Feb 4;21(2):e12987. Epub 2019 Jan 4.

Departments of Pediatrics and Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.

The relapsing fever spirochete Borrelia turicatae possesses a complex life cycle in its soft-bodied tick vector, Ornithodoros turicata. Spirochetes enter the tick midgut during a blood meal, and, during the following weeks, spirochetes disseminate throughout O. turicata. Read More

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http://dx.doi.org/10.1111/cmi.12987DOI Listing
February 2019
3 Reads

Chlamydia trachomatis impairs host base excision repair by downregulating polymerase β.

Cell Microbiol 2018 Nov 24:e12986. Epub 2018 Nov 24.

Department of Microbiology, University of Wuerzburg Biocenter, Wuerzburg, Germany.

Chlamydia trachomatis infections have been associated with ovarian cancer by several epidemiological studies. Here, we show that C. trachomatis-infected primary human ovarian epithelial cells display elevated oxidative DNA damage. Read More

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http://doi.wiley.com/10.1111/cmi.12986
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http://dx.doi.org/10.1111/cmi.12986DOI Listing
November 2018
14 Reads

Bartonella quintana type IV secretion effector BepE-induced selective autophagy by conjugation with K63 polyubiquitin chain.

Cell Microbiol 2018 Nov 21:e12984. Epub 2018 Nov 21.

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Bartonella effector proteins (named Beps) are substrates of VirB type IV secretion system for translocation into host cells evolved in Bartonella spp. Among these, BepE has been shown to protect cells from fragmentation effects triggered by other Beps and to promote in vivo dissemination of bacteria from the dermal site of inoculation to the bloodstream. Bacterial pathogens secreted effectors to modulate the interplay with host autophagy, either to combat autophagy to escape its bactericidal effect or to exploit autophagy to benefit intracellular replication. Read More

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http://dx.doi.org/10.1111/cmi.12984DOI Listing
November 2018
20 Reads
4.920 Impact Factor

MiRNA-218 regulates osteoclast differentiation and inflammation response in periodontitis rats through Mmp9.

Cell Microbiol 2018 Nov 16:e12979. Epub 2018 Nov 16.

Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.

Periodontitis is a multiple infection and inflammatory disease featured by connective tissue homeostasis loss, periodontal inflammation, and alveolar bone resorption. MicroRNAs (miRNAs) are involved in the mediation of a large scale of pathological processes. Here, we show that miRNA-218 provides protective effect on periodontitis via regulation of matrix metalloproteinase-9 (Mmp9). Read More

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http://doi.wiley.com/10.1111/cmi.12979
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http://dx.doi.org/10.1111/cmi.12979DOI Listing
November 2018
11 Reads

Photorhabdus luminescens Tc toxin is inhibited by the protease inhibitor MG132 and activated by protease cleavage resulting in increased binding to target cells.

Cell Microbiol 2019 Mar 2;21(3):e12978. Epub 2018 Dec 2.

Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Photorhabdus luminescens Tc toxins consist of the cell-binding component TcA, the linker component TcB, and the enzyme component TcC. TccC3, a specific isoform of TcC, ADP-ribosylates actin and causes redistribution of the actin cytoskeleton. TccC5, another isoform of TcC, ADP-ribosylates and activates Rho proteins. Read More

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http://dx.doi.org/10.1111/cmi.12978DOI Listing
March 2019
1 Read

LAMTOR2/LAMTOR1 complex is required for TAX1BP1-mediated xenophagy.

Cell Microbiol 2018 Nov 14:e12981. Epub 2018 Nov 14.

Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Xenophagy, also known as antibacterial autophagy, plays a role in host defence against invading pathogens such as Group A Streptococcus (GAS) and Salmonella. In xenophagy, autophagy receptors are used in the recognition of invading pathogens and in autophagosome maturation and autolysosome formation. However, the mechanism by which autophagy receptors are regulated during bacterial infection remains poorly elucidated. Read More

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http://doi.wiley.com/10.1111/cmi.12981
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http://dx.doi.org/10.1111/cmi.12981DOI Listing
November 2018
18 Reads

Processing and targeting of cathepsin L (TbCatL) to the lysosome in Trypanosoma brucei.

Cell Microbiol 2018 Nov 14:e12980. Epub 2018 Nov 14.

Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), Buffalo, New York, USA.

Cathepsin L (TbCatL) is an essential lysosomal thiol protease in African trypanosomes. TbCatL is synthesized as two precursor forms (P/X) that are activated to mature form (M) with the removal of the prodomain upon arrival in the lysosome. We examine TbCatL trafficking in a novel system: truncated TbCatL reporter without the C-terminal domain (CTD; TbCatL∆) ectopically expressed in an RNA interference (RNAi) cell line targeting the CTD/3' untranslated region (UTR) of endogenous mRNA. Read More

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http://doi.wiley.com/10.1111/cmi.12980
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http://dx.doi.org/10.1111/cmi.12980DOI Listing
November 2018
9 Reads

Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages.

Cell Microbiol 2019 Mar 7;21(3):e12976. Epub 2018 Dec 7.

Department of General Microbiology, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Recognition and internalisation of intracellular pathogens by host cells is a multifactorial process, involving both stable and transient interactions. The plasticity of the host cell plasma membrane is fundamental in this infectious process. Here, the participation of macrophage lipid microdomains during adhesion and internalisation of the fungal pathogen Histoplasma capsulatum (Hc) was investigated. Read More

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http://dx.doi.org/10.1111/cmi.12976DOI Listing
March 2019
3 Reads

Epithelial invasion by Salmonella Typhi using STIV-Met interaction.

Cell Microbiol 2019 Mar 12;21(3):e12982. Epub 2018 Dec 12.

Division of Clinical Medicine, National Institute of Cholera and Enteric Diseases, Kolkata, India.

Typhoid is a life-threatening febrile illness that affects ~24.2 million people worldwide and is caused by the intracellular bacteria Salmonella Typhi (S. Typhi). Read More

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http://doi.wiley.com/10.1111/cmi.12982
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March 2019
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Porphyromonas gingivalis induces the production of interleukin-31 by human mast cells, resulting in dysfunction of the gingival epithelial barrier.

Cell Microbiol 2019 Mar 28;21(3):e12972. Epub 2018 Nov 28.

Department of Oral Disease Research, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan.

Interleukin (IL)-31 is important for innate immunity in mucosal tissues and skin, and increased IL-31 expression participates in the pathogenesis of chronic inflammatory diseases affecting the skin, airways, lungs, and intestines. We investigated the contribution of mast cells to the induction of IL-31 production following infection with the periodontal pathogen, Porphyromonas gingivalis. We found that oral infection with P. Read More

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March 2019
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Klebsiella pneumoniae disassembles host microtubules in lung epithelial cells.

Cell Microbiol 2019 Mar 22;21(3):e12977. Epub 2018 Nov 22.

Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.

Klebsiella pneumoniae raises significant concerns to the health care industry as these microbes are the source of widespread contamination of medical equipment, cause pneumonia as well as other multiorgan metastatic infections and have gained multidrug resistance. Despite soaring mortality rates, the host cell alterations occurring during these infections remain poorly understood. Here, we show that during in vitro and in vivo K. Read More

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March 2019
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Shigella effector IpaH4.5 targets 19S regulatory particle subunit RPN13 in the 26S proteasome to dampen cytotoxic T lymphocyte activation.

Cell Microbiol 2019 Mar 5;21(3):e12974. Epub 2018 Dec 5.

Research Department, Nippon Institute for Biological Science, Tokyo, Japan.

Subversion of antigen-specific immune responses by intracellular pathogens is pivotal for successful colonisation. Bacterial pathogens, including Shigella, deliver effectors into host cells via the type III secretion system (T3SS) in order to manipulate host innate and adaptive immune responses, thereby promoting infection. However, the strategy for subverting antigen-specific immunity is not well understood. Read More

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March 2019
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Neutrophils and IL17A mediate flagellar hook protein FlgE-induced mouse acute lung inflammation.

Cell Microbiol 2019 Mar 5;21(3):e12975. Epub 2018 Dec 5.

MOH Key Lab of Thrombosis and Hemostasis, Collaborative Innovation Center of Hematology-Thrombosis and Hemostasis Group, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, College of Medicine, Soochow University, Suzhou, China.

Bacterial flagellar hook and recombinant flagellar hook protein E (FlgE) were reportedly immunostimulatory in mammalian cells or tissues. Current study focused on the mechanisms underlying FlgE stimulation. In an acute lung injury model induced by intranasal FlgE challenge, neutrophils were the predominant infiltrates in lungs, and depletion of neutrophils with anti-Ly6G antibody attenuated FlgE-induced lung damage. Read More

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March 2019
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Theileria highjacks JNK2 into a complex with the macroschizont GPI (GlycosylPhosphatidylInositol)-anchored surface protein p104.

Cell Microbiol 2019 Mar 5;21(3):e12973. Epub 2018 Dec 5.

Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Faculté de Médecine, Université Paris Descartes, Sorbonne Paris Cité, Paris, 75014, France.

Constitutive c-Jun N-terminal kinase (JNK) activity characterizes bovine T and B cells infected with Theileria parva, and B cells and macrophages infected with Theileria annulata. Here, we show that T. annulata infection of macrophages manipulates JNK activation by recruiting JNK2 and not JNK1 to the parasite surface, whereas JNK1 is found predominantly in the host cell nucleus. Read More

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March 2019
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Characterisation of infection associated microRNA and protein cargo in extracellular vesicles of Theileria annulata infected leukocytes.

Cell Microbiol 2019 Jan 9;21(1):e12969. Epub 2018 Nov 9.

Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow, UK.

The protozoan parasites Theileria annulata and Theileria parva are unique amongst intracellular eukaryotic pathogens as they induce a transformation-like phenotype in their bovine host cell. T. annulata causes tropical theileriosis, which is frequently fatal, with infected leukocytes becoming metastatic and forming foci in multiple organs resulting in destruction of the lymphoid system. Read More

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January 2019
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Evasion of phagotrophic predation by protist hosts and innate immunity of metazoan hosts by Legionella pneumophila.

Cell Microbiol 2019 Jan 15;21(1):e12971. Epub 2018 Nov 15.

Department of Microbiology and Immunology, College of Medicine, University of Louisville, Louisville, Kentucky.

Legionella pneumophila is a ubiquitous environmental bacterium that has evolved to infect and proliferate within amoebae and other protists. It is thought that accidental inhalation of contaminated water particles by humans is what has enabled this pathogen to proliferate within alveolar macrophages and cause pneumonia. However, the highly evolved macrophages are equipped with more sophisticated innate defence mechanisms than are protists, such as the evolution of phagotrophic feeding into phagocytosis with more evolved innate defence processes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296878PMC
January 2019
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Gamma-glutamyltranspeptidase expression by Helicobacter saguini, an enterohepatic Helicobacter species isolated from cotton top tamarins with chronic colitis.

Cell Microbiol 2019 Mar 14;21(3):e12968. Epub 2018 Nov 14.

Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Background: Helicobacter saguini is a novel enterohepatic Helicobacter species isolated from captive cotton top tamarins with chronic colitis and colon cancer. Monoassociated H. saguini infection in gnotobiotic IL-10 mice causes typhlocolitis and dysplasia; however, the virulent mechanisms of this species are unknown. Read More

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March 2019
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Synergistic roles of acyl-CoA binding protein (ACBP1) and sterol carrier protein 2 (SCP2) in Toxoplasma lipid metabolism.

Cell Microbiol 2019 Mar 22;21(3):e12970. Epub 2018 Nov 22.

National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Toxoplasma gondii relies on apicoplast-localised FASII pathway and endoplasmic reticulum-associated fatty acid elongation pathway for the synthesis of fatty acids, which flow through lipid metabolism mainly in the form of long-chain acyl-CoA (LCACoAs) esters. Functions of Toxoplasma acyl-CoA transporters in lipid metabolism remain unclear. Here, we investigated the roles of acyl-CoA-binding protein (TgACBP1) and a sterol carrier protein-2 (TgSCP2) as cytosolic acyl-CoA transporters in lipid metabolism. Read More

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March 2019
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Aggregatibacter actinomycetemcomitans leukotoxin causes activation of lymphocyte function-associated antigen 1.

Cell Microbiol 2019 Mar 28;21(3):e12967. Epub 2018 Nov 28.

Departments of Pathology, School of Dental Medicine, and the Division of Hematology/Oncology, University of Pennsylvania, Philadelphia, Pennsylvania.

Repeats-in-toxin leukotoxin (LtxA) produced by the oral bacterium Aggregatibacter actinomycetemcomitans kills human leukocytes in a lymphocyte function-associated antigen 1 (LFA-1, integrin α /β )-dependent manner, although the mechanism for this interaction has not been identified. The LtxA internalisation by LFA-1-expressing cells was explored with florescence resonance energy transfer (FRET) microscopy using a cell line that expresses LFA-1 with a cyan fluorescent protein-tagged cytosolic α domain and a yellow fluorescent protein-tagged β domain. Phorbol 12-myristate 13-acetate activation of LFA-1 caused transient cytosolic domain separation. Read More

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March 2019
9 Reads