2,629 results match your criteria Cellular microbiology[Journal]


Cholix toxin, an eEF2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.

Cell Microbiol 2019 Apr 22:e13033. Epub 2019 Apr 22.

Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Vibrio cholerae produced-Cholix toxin (Cholix) is a cytotoxin that ADP-ribosylates eukaryotic elongation factor 2, inhibiting protein synthesis, and inducing apoptosis. Here, we identified prohibitin (PHB) 1 and 2 as novel Cholix-interacting membrane proteins in immortalized human hepatocytes and HepG2 cells by Cholix immunoprecipitation assays. The expression level of PHB1 was decreased by Cholix after a 12 h incubation. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13033
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http://dx.doi.org/10.1111/cmi.13033DOI Listing
April 2019
1 Read

Haemophilus parasuis infection in 3D4/21 cells induces autophagy through the AMPK pathway.

Cell Microbiol 2019 Apr 11:e13031. Epub 2019 Apr 11.

Key Laboratory of Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Haemophilus parasuis (H. parasuis) is a common commensal in the upper respiratory tract of pigs, but causes Glässer's disease in stress conditions. To date, many studies focused on the immune evasion and virulence of H. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13031
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http://dx.doi.org/10.1111/cmi.13031DOI Listing
April 2019
5 Reads

Disulfide bond of Mycoplasma pneumoniae community acquired respiratory distress syndrome toxin is essential to maintain the ADP-ribosylating and vacuolating activities.

Cell Microbiol 2019 Apr 11:e13032. Epub 2019 Apr 11.

Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health Science Center at San Antonio, TX.

Mycoplasma pneumoniae is the leading cause of bacterial community acquired pneumonia among hospitalized children in United States and worldwide. Community acquired respiratory distress syndrome (CARDS) toxin is a key virulence determinant of M. pneumoniae. Read More

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http://dx.doi.org/10.1111/cmi.13032DOI Listing
April 2019
1 Read

Neutralizing antibodies block the function of Rh5/Ripr/CyRPA complex during invasion of Plasmodium falciparum into human erythrocytes.

Cell Microbiol 2019 Apr 9:e13030. Epub 2019 Apr 9.

Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.

An effective vaccine is a priority for malaria control and elimination. The leading candidate in the Plasmodium falciparum blood stage is PfRh5. PfRh5 assembles into trimeric complex with PfRipr and PfCyRPA in the parasite and this complex is essential for erythrocyte invasion. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13030
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http://dx.doi.org/10.1111/cmi.13030DOI Listing
April 2019
2 Reads
4.915 Impact Factor

A small intergenic region of lp17 is required for evasion of adaptive immunity and induction of pathology by the Lyme disease spirochete.

Cell Microbiol 2019 Apr 3:e13029. Epub 2019 Apr 3.

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.

The causative agent of Lyme disease, Borrelia burgdorferi, harbours a single linear chromosome and upwards of 23 linear and circular plasmids. Only a minority of these plasmids, including linear plasmid 17, are maintained with near-absolute fidelity during extended in vitro passage, and characterisation of any putative virulence determinants they encode has only recently begun. In this work, a mutant lacking a ~4. Read More

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http://dx.doi.org/10.1111/cmi.13029DOI Listing
April 2019
4 Reads

Eimeria tenella ROP kinase EtROP1 induces G0/G1 cell cycle arrest and inhibits host cell apoptosis.

Cell Microbiol 2019 Apr 2:e13027. Epub 2019 Apr 2.

Infectiologie et Santé Publique, INRA, Université de Tours, UMR 1282, Nouzilly, France.

Coccidia are obligate intracellular protozoan parasites responsible for human and veterinary diseases. Eimeria tenella, the etiologic agent of cecal coccidiosis, is a major pathogen of chickens. In Toxoplasma gondii, some kinases from the rhoptry compartment (ROP) are key virulence factors. Read More

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http://dx.doi.org/10.1111/cmi.13027DOI Listing
April 2019
1 Read

The Plasmodium berghei serine protease PbSUB1 plays an important role in male gamete egress.

Cell Microbiol 2019 Apr 2:e13028. Epub 2019 Apr 2.

Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy.

The Plasmodium subtilisin-like serine protease SUB1 is expressed in hepatic and both asexual and sexual blood parasite stages. SUB1 is required for egress of invasive forms of the parasite from both erythrocytes and hepatocytes, but its subcellular localization, function and potential substrates in the sexual stages are unknown. Here we have characterized the expression profile and subcellular localization of SUB1 in P. Read More

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http://dx.doi.org/10.1111/cmi.13028DOI Listing

TLR9 Limits Enteric Antimicrobial Responses and Promotes Microbiota Based Colonization Resistance during Citrobacter rodentium Infection.

Cell Microbiol 2019 Mar 20:e13026. Epub 2019 Mar 20.

Division of Gastroenterology, Hepatology and Nutrition, BC Children's Hospital Research Institute and the University of British Columbia, Vancouver, Canada.

Mammalian cells express an array of toll-like receptors (TLRs) to detect and respond to microbial pathogens, including enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). These clinically important attaching and effacing (A/E) pathogens infect the apical surface of intestinal epithelial cells, causing inflammation as well as severe diarrheal disease. Read More

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http://dx.doi.org/10.1111/cmi.13026DOI Listing
March 2019
1 Read

Co-opting oxylipin signals in microbial disease.

Cell Microbiol 2019 Mar 13:e13025. Epub 2019 Mar 13.

Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin.

Oxylipins, or oxygenated lipids, are universal signaling molecules across all kingdoms of life. These molecules, either produced by microbial pathogens or their mammalian host, regulate inflammation during microbial infection. In this review, we summarize current literature on the biosynthesis pathways of microbial oxylipins and their biological activity towards mammalian cells. Read More

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http://doi.wiley.com/10.1111/cmi.13025
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http://dx.doi.org/10.1111/cmi.13025DOI Listing
March 2019
2 Reads

Histamine releasing factor and elongation factor 1 alpha secreted via malaria parasites extracellular vesicles promote immune evasion by inhibiting specific T cell responses.

Cell Microbiol 2019 Mar 5:e13021. Epub 2019 Mar 5.

Institut Pasteur, Unité de Biologie des Interactions Hôte Parasites, Paris, France.

Protozoan pathogens secrete nanosized particles called extracellular vesicles (EVs) to facilitate their survival and chronic infection. Here, we show the inhibition by Plasmodium berghei NK65 blood stage-derived EVs of the proliferative response of CD4 T cells in response to antigen presentation. Importantly, these results were confirmed in vivo by the capacity of EVs to diminish the ovalbumin-specific delayed type hypersensitivity response. Read More

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http://dx.doi.org/10.1111/cmi.13021DOI Listing
March 2019
2 Reads

Intravital microscopy: Imaging host-parasite interactions in the brain.

Cell Microbiol 2019 May 28;21(5):e13024. Epub 2019 Mar 28.

Parasitology-Centre for Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany.

Intravital fluorescence microscopy (IVM) is a powerful technique for imaging multiple organs, including the brain of living mice and rats. It enables the direct visualisation of cells in situ providing a real-life view of biological processes that in vitro systems cannot. In addition, to the technological advances in microscopy over the last decade, there have been supporting innovations in data storage and analytical packages that enable the visualisation and analysis of large data sets. Read More

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http://dx.doi.org/10.1111/cmi.13024DOI Listing
May 2019
4 Reads

Intravital imaging of host-parasite interactions in skin and adipose tissues.

Cell Microbiol 2019 May 3;21(5):e13023. Epub 2019 Apr 3.

Integrative Parasitology, Centre for Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany.

Intravital microscopy allows the visualisation of how pathogens interact with host cells and tissues in living animals in real time. This method has enabled key advances in our understanding of host-parasite interactions under physiological conditions. A combination of genetics, microscopy techniques, and image analysis have recently facilitated the understanding of biological phenomena in living animals at cellular and subcellular resolution. Read More

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http://dx.doi.org/10.1111/cmi.13023DOI Listing

How many protein molecules are secreted by single Helicobacter pylori cells: Quantification of serine protease HtrA.

Cell Microbiol 2019 Mar 1:e13022. Epub 2019 Mar 1.

Department of Biology, Division of Microbiology, Friedrich Alexander University Erlangen, Erlangen, Germany.

Infection with Helicobacter pylori represents a major risk for developing peptic ulcer disease, gastric adenocarcinoma, and various other gastric and nongastric sicknesses. A series of H. pylori virulence factors can be secreted into the cell culture supernatant, and the secretome contains more than 100 different proteins. Read More

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http://dx.doi.org/10.1111/cmi.13022DOI Listing
March 2019
10 Reads

Urinary exosome as a potential biomarker for urinary tract infection.

Cell Microbiol 2019 Feb 28:e13020. Epub 2019 Feb 28.

Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Unlike urinary tract infection (UTI), asymptomatic bacteriuria (ABU) should not be treated, with some exceptions such as pregnant women and patients who will undergo traumatic urologic interventions. However, there has been no clinically available marker for their differential diagnosis. Exosomes or small extracellular vesicles carry proteins contained in cells from which they are derived, thus having the potential as a biomarker of several diseases. Read More

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http://dx.doi.org/10.1111/cmi.13020DOI Listing
February 2019

Infection by Brucella melitensis or Brucella papionis modifies essential physiological functions of human trophoblasts.

Cell Microbiol 2019 Feb 28:e13019. Epub 2019 Feb 28.

VBMI, INSERM, Université de Montpellier, Nîmes, France.

Brucellosis is a zoonosis caused by bacteria of the Brucella genus. In ruminants, brucellosis causes abortion, followed by chronic infection and secretion of bacteria in milk. In humans, it usually presents as flu-like symptoms, with serious complications if untreated. Read More

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http://dx.doi.org/10.1111/cmi.13019DOI Listing
February 2019
2 Reads

Biogenesis and secretion of micronemes in Toxoplasma gondii.

Cell Microbiol 2019 May 18;21(5):e13018. Epub 2019 Mar 18.

Department of Microbiology and Molecular Medicine, University of Geneva CMU, Geneva, Switzerland.

One of the hallmarks of the parasitic phylum of Apicomplexa is the presence of highly specialised, apical secretory organelles, called the micronemes and rhoptries that play critical roles in ensuring survival and dissemination. Upon exocytosis, the micronemes release adhesin complexes, perforins, and proteases that are crucially implicated in egress from infected cells, gliding motility, migration across biological barriers, and host cell invasion. Recent studies on Toxoplasma gondii and Plasmodium species have shed more light on the signalling events and the machinery that trigger microneme secretion. Read More

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http://dx.doi.org/10.1111/cmi.13018DOI Listing

α-Haemolysin production, as a single factor, causes fulminant sepsis in a model of Escherichia coli-induced bacteraemia.

Cell Microbiol 2019 Feb 13:e13017. Epub 2019 Feb 13.

Department of Biomedicine, Aarhus University, Aarhus C, Denmark.

α-Haemolysin (HlyA) from uropathogenic Escherichia coli has been demonstrated to be a significant virulence factor for ascending urinary tract infections. Once the E. coli reach the well-vascularised kidneys, there is a high risk of bacteraemia and a subsequent septic host response. Read More

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http://dx.doi.org/10.1111/cmi.13017DOI Listing
February 2019
2 Reads

Integrity under stress: Host membrane remodelling and damage by fungal pathogens.

Cell Microbiol 2019 Apr 7;21(4):e13016. Epub 2019 Mar 7.

Keenan Research Centre for Biomedical Sciences, St. Michael's Hospital, Toronto, Ontario, Canada.

Membrane bilayers of eukaryotic cells are an amalgam of lipids and proteins that distinguish organelles and compartmentalise cellular functions. The mammalian cell has evolved mechanisms to sense membrane tension or damage and respond as needed. In the case of the plasma membrane and phagosomal membrane, these bilayers act as a barrier to microorganisms and are a conduit by which the host interacts with pathogens, including fungi such as Candida, Cryptococcus, Aspergillus, or Histoplasma species. Read More

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http://dx.doi.org/10.1111/cmi.13016DOI Listing
April 2019
3 Reads

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Cell Microbiol 2019 Jan 31:e13014. Epub 2019 Jan 31.

School of life sciences, Sun Yat-sen University, Guangzhou, China.

The major virulence determinant of Legionella pneumophila is the type IVB secretion system (T4BSS), which delivers approximately 330 effector proteins into the host cell to modulate various cellular processes. However, the functions of most effector proteins remain unclear. WipA, an effector, was the first phosphotyrosine phosphatase of Legionella with unknown function. Read More

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http://dx.doi.org/10.1111/cmi.13014DOI Listing
January 2019
2 Reads

Mycoplasma pneumoniae protects infected epithelial cells from hydrogen peroxide-induced cell detachment.

Cell Microbiol 2019 Jan 31:e13015. Epub 2019 Jan 31.

Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, Kurume, Japan.

Epithelial cell shedding is a defence mechanism against infectious microbes that use these cells as an infection foothold and that eliminate microbes from infection foci by removing infected cells. Mycoplasma pneumoniae, a causative agent of respiratory infections, is known to adhere to and colonise the surface of ciliated airway epithelial cells; it produces a large amount of hydrogen peroxide, indicating its capability of regulating hydrogen peroxide-induced infected cell detachment. In this study, we found that M. Read More

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http://dx.doi.org/10.1111/cmi.13015DOI Listing
January 2019
4.915 Impact Factor

Dysregulated haemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish.

Cell Microbiol 2019 Jan 31:e13010. Epub 2019 Jan 31.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

Inflammasomes are important innate immune components in mammals. However, the bacterial factors modulating inflammasome activation in fish, and the mechanisms by which they alter fish immune defences, remain to be investigated. In this work, a mutant of the fish pathogen Edwardsiella piscicida (E. Read More

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http://dx.doi.org/10.1111/cmi.13010DOI Listing
January 2019
2 Reads

SH3-class Ras guanine nucleotide exchange factors are essential for Aspergillus fumigatus invasive growth.

Cell Microbiol 2019 Jan 30:e13013. Epub 2019 Jan 30.

Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Proper hyphal morphogenesis is essential for the establishment and progression of invasive disease caused by filamentous fungi. In the human pathogen Aspergillus fumigatus, signalling cascades driven by Ras and Ras-like proteins orchestrate a wide variety of cellular processes required for hyphal growth. For activation, these proteins require interactions with Ras-subfamily-specific guanine nucleotide exchange factors (RasGEFs). Read More

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http://dx.doi.org/10.1111/cmi.13013DOI Listing
January 2019
2 Reads

Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli.

Cell Microbiol 2019 Jan 23:e13012. Epub 2019 Jan 23.

Norwich Medical School, University of East Anglia, Norwich, UK.

Enteroaggregative Escherichia coli (EAEC) are important intestinal pathogens causing acute and persistent diarrhoeal illness worldwide. Although many putative EAEC virulence factors have been identified, their association with pathogenesis remains unclear. As environmental cues can modulate bacterial virulence, we investigated the effect of oxygen and human intestinal epithelium on EAEC virulence gene expression to determine the involvement of respective gene products in intestinal colonisation and pathogenesis. Read More

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http://dx.doi.org/10.1111/cmi.13012DOI Listing
January 2019

Calcium negatively regulates secretion from dense granules in Toxoplasma gondii.

Cell Microbiol 2019 Jan 23:e13011. Epub 2019 Jan 23.

Department of Biochemistry, University of Cambridge, Cambridge, UK.

Apicomplexan parasites including Toxoplasma gondii and Plasmodium spp. manufacture a complex arsenal of secreted proteins used to interact with and manipulate their host environment. These proteins are organised into three principle exocytotic compartment types according to their functions: micronemes for extracellular attachment and motility, rhoptries for host cell penetration, and dense granules for subsequent manipulation of the host intracellular environment. Read More

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http://doi.wiley.com/10.1111/cmi.13011
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http://dx.doi.org/10.1111/cmi.13011DOI Listing
January 2019
7 Reads

Distinct Mycobacterium marinum phosphatases determine pathogen vacuole phosphoinositide pattern, phagosome maturation, and escape to the cytosol.

Cell Microbiol 2019 Jan 17:e13008. Epub 2019 Jan 17.

Faculty of Medicine, Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.

The causative agent of tuberculosis, Mycobacterium tuberculosis, and its close relative Mycobacterium marinum manipulate phagocytic host cells, thereby creating a replication-permissive compartment termed the Mycobacterium-containing vacuole (MCV). The phosphoinositide (PI) lipid pattern is a crucial determinant of MCV formation and is targeted by mycobacterial PI phosphatases. In this study, we establish an efficient phage transduction protocol to construct defined M. Read More

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http://doi.wiley.com/10.1111/cmi.13008
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http://dx.doi.org/10.1111/cmi.13008DOI Listing
January 2019
7 Reads

Illuminating how malaria parasites export proteins into host erythrocytes.

Cell Microbiol 2019 Apr 12;21(4):e13009. Epub 2019 Feb 12.

School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia.

Plasmodium parasites that cause the disease malaria have developed an elaborate trafficking pathway to facilitate the export of hundreds of effector proteins into their host cell, the erythrocyte. In this review, we outline how certain effector proteins contribute to parasite survival, virulence, and immune evasion. We also highlight how parasite proteins destined for export are recognised at the endoplasmic reticulum to facilitate entry into the export pathway and how the effector proteins are able to transverse the bounding parasitophorous vaculoar membrane via the Plasmodium translocon of exported proteins to gain access to the host cell. Read More

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http://dx.doi.org/10.1111/cmi.13009DOI Listing
April 2019
1 Read

Cellular microbiology interview-Dr. Jason King.

Authors:
Jason S King

Cell Microbiol 2019 Jan 16:e13007. Epub 2019 Jan 16.

Royal Society University Research Fellow, Department of Biomedical Science, The University of Sheffield, Sheffield, UK.

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http://dx.doi.org/10.1111/cmi.13007DOI Listing
January 2019
2 Reads

miR-193b represses influenza A virus infection by inhibiting Wnt/β-catenin signalling.

Cell Microbiol 2019 May 25;21(5):e13001. Epub 2019 Jan 25.

Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.

Due to an increasing emergence of new and drug-resistant strains of the influenza A virus (IAV), developing novel measures to combat influenza is necessary. We have previously shown that inhibiting Wnt/β-catenin pathway reduces IAV infection. In this study, we aimed to identify antiviral human microRNAs (miRNAs) that target the Wnt/β-catenin signalling pathway. Read More

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http://doi.wiley.com/10.1111/cmi.13001
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http://dx.doi.org/10.1111/cmi.13001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459727PMC
May 2019
21 Reads

The lipoprotein HP1454 of Helicobacter pylori regulates T-cell response by shaping T-cell receptor signalling.

Cell Microbiol 2019 May 31;21(5):e13006. Epub 2019 Jan 31.

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Helicobacter pylori (HP) is a Gram-negative bacterium that chronically infects the stomach of more than 50% of human population and represents a major cause of gastric cancer, gastric lymphoma, gastric autoimmunity, and peptic ulcer. It still remains to be elucidated, which HP virulence factors are important in the development of gastric disorders. Here, we analysed the role of the HP protein HP1454 in the host-pathogen interaction. Read More

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http://dx.doi.org/10.1111/cmi.13006DOI Listing
May 2019
7 Reads
4.915 Impact Factor

Role of distinct type-IV-secretion systems and secreted effector sets in host adaptation by pathogenic Bartonella species.

Cell Microbiol 2019 Mar 6;21(3):e13004. Epub 2019 Feb 6.

Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland.

The α-proteobacterial genus Bartonella comprises a large number of facultative intracellular pathogens that share a common lifestyle hallmarked by hemotrophic infection and arthropod transmission. Speciation in the four deep-branching lineages (L1-L4) occurred by host adaptation facilitating the establishment of long lasting bacteraemia in specific mammalian reservoir host(s). Two distinct type-IV-secretion systems (T4SSs) acquired horizontally by different Bartonella lineages mediate essential host interactions during infection and represent key innovations for host adaptation. Read More

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http://dx.doi.org/10.1111/cmi.13004DOI Listing
March 2019
5 Reads

Multimodal analysis of Plasmodium knowlesi-infected erythrocytes reveals large invaginations, swelling of the host cell, and rheological defects.

Cell Microbiol 2019 May 11;21(5):e13005. Epub 2019 Feb 11.

Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, Melbourne, Victoria, Australia.

The simian parasite Plasmodium knowlesi causes severe and fatal malaria infections in humans, but the process of host cell remodelling that underpins the pathology of this zoonotic parasite is only poorly understood. We have used serial block-face scanning electron microscopy to explore the topography of P. knowlesi-infected red blood cells (RBCs) at different stages of asexual development. Read More

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http://dx.doi.org/10.1111/cmi.13005DOI Listing
May 2019
5 Reads

Cellular microbiology interview-Dr. Melanie Blokesch.

Authors:
Melanie Blokesch

Cell Microbiol 2019 May 15;21(5):e13002. Epub 2019 Jan 15.

Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

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http://dx.doi.org/10.1111/cmi.13002DOI Listing
May 2019
1 Read

Host cell protein LAMP-2 is the receptor for Trypanosoma cruzi surface molecule gp82 that mediates invasion.

Cell Microbiol 2019 May 17;21(5):e13003. Epub 2019 Jan 17.

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

Host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is mediated by MT-specific surface molecule gp82, which binds to a still unidentified receptor, inducing lysosome spreading and exocytosis required for the parasitophorous vacuole formation. We examined the involvement of the major lysosome membrane-associated LAMP proteins in MT invasion. First, human epithelial HeLa cells were incubated with MT in the presence of antibody to LAMP-1 or LAMP-2. Read More

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http://dx.doi.org/10.1111/cmi.13003DOI Listing
May 2019
1 Read
4.915 Impact Factor

Transmission of the malaria parasite requires ferlin for gamete egress from the red blood cell.

Cell Microbiol 2019 May 18;21(5):e12999. Epub 2019 Jan 18.

Center for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, Heidelberg, Germany.

Ferlins mediate calcium-dependent vesicular fusion. Although conserved throughout eukaryotic evolution, their function in unicellular organisms including apicomplexan parasites is largely unknown. Here, we define a crucial role for a ferlin-like protein (FLP) in host-to-vector transmission of the rodent malaria parasite Plasmodium berghei. Read More

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http://doi.wiley.com/10.1111/cmi.12999
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http://dx.doi.org/10.1111/cmi.12999DOI Listing
May 2019
3 Reads

Targeting host lipid flows: Exploring new antiviral and antibiotic strategies.

Cell Microbiol 2019 Mar 17;21(3):e12996. Epub 2019 Jan 17.

Cell Structure Lab, National Centre for Biotechnology, CNB-CSIC, Madrid, Spain.

Bacteria and viruses pose serious challenges for humans because they evolve continuously. Despite ongoing efforts, antiviral drugs to treat many of the most troubling viruses have not been approved yet. The recent launch of new antimicrobials is generating hope as more and more pathogens around the world become resistant to available drugs. Read More

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http://dx.doi.org/10.1111/cmi.12996DOI Listing
March 2019
1 Read

The structure of the Shiga toxin 2a A-subunit dictates the interactions of the toxin with blood components.

Cell Microbiol 2019 May 18;21(5):e13000. Epub 2019 Jan 18.

Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.

Hemolytic uremic syndrome (eHUS) is a severe complication of human infections with Shiga toxins (Stxs)-producing Escherichia coli. A key step in the pathogenesis of eHUS is the interaction of Stxs with blood components before the targeting of renal endothelial cells. Here, we show that a single proteolytic cleavage in the Stx2a A-subunit, resulting into two fragments (A1 and A2) linked by a disulfide bridge (cleaved Stx2a), dictates different binding abilities. Read More

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http://doi.wiley.com/10.1111/cmi.13000
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http://dx.doi.org/10.1111/cmi.13000DOI Listing
May 2019
6 Reads

Discordant susceptibility of inbred C57BL/6 versus outbred CD1 mice to experimental fungal sepsis.

Cell Microbiol 2019 May 9;21(5):e12995. Epub 2019 Jan 9.

Immunoreceptors of the Innate and Adaptive System team, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.

Individual susceptibility differences to fungal infection following invasive and/or immunosuppressive medical interventions are an important clinical issue. In order to explore immune response-related factors that may be linked to fungal infection susceptibility, we have compared the response of inbred C57BL/6J and outbred CD1 mouse strains to different experimental models of fungal sepsis. The challenge of animals with the zymosan-induced generalised inflammation model revealed poorer survival rates in C57BL/6J, consistent with lower Th1 cytokine interferon (IFN)-γ serum levels, compared with CD1 mice. Read More

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http://dx.doi.org/10.1111/cmi.12995DOI Listing
May 2019
7 Reads

In or out: Phagosomal escape of Staphylococcus aureus.

Cell Microbiol 2019 Mar 18;21(3):e12997. Epub 2019 Jan 18.

Chair of Microbiology, University of Würzburg, Würzburg, Germany.

Staphylococcus aureus is internalised by host cells in vivo, and recent research results suggest that the bacteria use this intracellularity to persist in the host and form a reservoir for recurrent infections. However, in different cells types, the pathogen resorts to alternative strategies to survive phagocytosis and the antimicrobial mechanisms of host cells. In non-professional phagocytes, S. Read More

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http://dx.doi.org/10.1111/cmi.12997DOI Listing
March 2019
1 Read

Blood treatment of Lyme borreliae demonstrates the mechanism of CspZ-mediated complement evasion to promote systemic infection in vertebrate hosts.

Cell Microbiol 2019 Feb 7;21(2):e12998. Epub 2019 Jan 7.

Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY, USA.

Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most common vector-borne disease in the United States and Europe. The spirochetes are transmitted from mammalian and avian reservoir hosts to humans via ticks. Following tick bites, spirochetes colonize the host skin and then disseminate haematogenously to various organs, a process that requires this pathogen to evade host complement, an innate immune defence system. Read More

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http://doi.wiley.com/10.1111/cmi.12998
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http://dx.doi.org/10.1111/cmi.12998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336514PMC
February 2019
14 Reads

Cellular microbiology interview-Dr Marek Basler.

Authors:
Marek Basler

Cell Microbiol 2019 Apr 11;21(4):e12991. Epub 2019 Jan 11.

Biozentrum, Universitat Basel, Switzerland.

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http://dx.doi.org/10.1111/cmi.12991DOI Listing
April 2019
1 Read

The puzzling construction of the conidial outer layer of Aspergillus fumigatus.

Cell Microbiol 2019 May 18;21(5):e12994. Epub 2019 Jan 18.

Aspergillus Unit, Institut Pasteur, Paris, France.

If the mycelium of Aspergillus fumigatus is very short-lived in the laboratory, conidia can survive for years. This survival capacity and extreme resistance to environmental insults is a major biological characteristic of this fungal species. Moreover, conidia, which easily reach the host alveola, are the infective propagules. Read More

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May 2019
18 Reads

Infection of HeLa cells with Chlamydia trachomatis inhibits protein synthesis and causes multiple changes to host cell pathways.

Cell Microbiol 2019 Apr 7;21(4):e12993. Epub 2019 Jan 7.

Institute for Microbiology and Hygiene, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

The obligate intracellular bacterium Chlamydia trachomatis replicates in a cytosolic vacuole in human epithelial cells. Infection of human cells with C. trachomatis causes substantial changes to many host cell-signalling pathways, but the molecular basis of such influence is not well understood. Read More

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April 2019
4 Reads

Interferon-gamma production in Lyme arthritis synovial tissue promotes differentiation of fibroblast-like synoviocytes into immune effector cells.

Cell Microbiol 2019 Feb 4;21(2):e12992. Epub 2019 Jan 4.

Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.

Lyme arthritis (LA), a late disease manifestation of Borrelia burgdorferi infection, usually resolves with antibiotic therapy. However, some patients develop proliferative synovitis lasting months to several years after spirochetal killing, called postinfectious LA. In this study, we phenotyped haematopoietic and stromal cell populations in the synovial lesion ex vivo and used these findings to generate an in vitro model of LA using patient-derived fibroblast-like synoviocytes (FLS). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336510PMC
February 2019
5 Reads

Leptospira species promote a pro-inflammatory phenotype in human neutrophils.

Cell Microbiol 2019 Feb 21;21(2):e12990. Epub 2018 Dec 21.

Laboratory of Animal Viruses, Institute of Biotechnology and Molecular Biology, CONICET-UNLP, La Plata, Argentina.

Leptospirosis is a global zoonosis caused by pathogenic Leptospira. Neutrophils are key cells against bacterial pathogens but can also contribute to tissue damage. Because the information regarding the role of human neutrophils in leptospirosis is scant, we comparatively analysed the human neutrophil's response to saprophytic Leptospira biflexa serovar Patoc (Patoc) and the pathogenic Leptospira interrogans serovar Copenhageni (LIC). Read More

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February 2019
4 Reads

Acetylation is the most abundant actin modification in Entamoeba histolytica and modifications of actin's amino-terminal domain change cytoskeleton activities.

Cell Microbiol 2019 Apr 21;21(4):e12983. Epub 2018 Dec 21.

Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France.

Actin is one of the most conserved, abundant, and ubiquitous proteins in all eukaryotes characterised to date. Posttranslation modifications of actin modify the organisation of the actin-rich cytoskeleton. In particular, chemical modifications of actin's amino-terminal region determine how filamentous actin is organised into scaffolds. Read More

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April 2019
2 Reads

The Class IV human deacetylase, HDAC11, exhibits anti-influenza A virus properties via its involvement in host innate antiviral response.

Cell Microbiol 2019 Apr 21;21(4):e12989. Epub 2018 Dec 21.

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.

Histone deacetylase 11 (HDAC11) is most recently discovered deacetylase. Here, we demonstrate that human HDAC11 exhibits anti-influenza A virus (IAV) properties. We found that knockdown of HDAC11 expression augments IAV growth kinetics in human lung epithelial cells A549 by up to 1 log. Read More

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http://doi.wiley.com/10.1111/cmi.12989
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April 2019
13 Reads

Listeriolysin O: A phagosome-specific cytolysin revisited.

Cell Microbiol 2019 Mar 15;21(3):e12988. Epub 2019 Jan 15.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California.

Listeriolysin O (LLO) is an essential determinant of Listeria monocytogenes pathogenesis that mediates the escape of L. monocytogenes from host cell vacuoles, thereby allowing replication in the cytosol without causing appreciable cell death. As a member of the cholesterol-dependent cytolysin (CDC) family of pore-forming toxins, LLO is unique in that it is secreted by a facultative intracellular pathogen, whereas all other CDCs are produced by pathogens that are largely extracellular. Read More

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March 2019
14 Reads

The relapsing fever spirochete Borrelia turicatae persists in the highly oxidative environment of its soft-bodied tick vector.

Cell Microbiol 2019 Feb 4;21(2):e12987. Epub 2019 Jan 4.

Departments of Pediatrics and Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.

The relapsing fever spirochete Borrelia turicatae possesses a complex life cycle in its soft-bodied tick vector, Ornithodoros turicata. Spirochetes enter the tick midgut during a blood meal, and, during the following weeks, spirochetes disseminate throughout O. turicata. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454574PMC
February 2019
3 Reads

Chlamydia trachomatis impairs host base excision repair by downregulating polymerase β.

Cell Microbiol 2019 Apr 9;21(4):e12986. Epub 2019 Jan 9.

Department of Microbiology, University of Wuerzburg Biocenter, Wuerzburg, Germany.

Chlamydia trachomatis infections have been associated with ovarian cancer by several epidemiological studies. Here, we show that C. trachomatis-infected primary human ovarian epithelial cells display elevated oxidative DNA damage. Read More

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http://doi.wiley.com/10.1111/cmi.12986
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April 2019
19 Reads

Bartonella quintana type IV secretion effector BepE-induced selective autophagy by conjugation with K63 polyubiquitin chain.

Cell Microbiol 2019 Apr 13;21(4):e12984. Epub 2018 Dec 13.

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Bartonella effector proteins (named Beps) are substrates of VirB type IV secretion system for translocation into host cells evolved in Bartonella spp. Among these, BepE has been shown to protect cells from fragmentation effects triggered by other Beps and to promote in vivo dissemination of bacteria from the dermal site of inoculation to the bloodstream. Bacterial pathogens secreted effectors to modulate the interplay with host autophagy, either to combat autophagy to escape its bactericidal effect or to exploit autophagy to benefit intracellular replication. Read More

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April 2019
30 Reads
4.920 Impact Factor