5,776 results match your criteria Cellular and molecular life sciences : CMLS[Journal]


Immunosenescence: the potential role of myeloid-derived suppressor cells (MDSC) in age-related immune deficiency.

Cell Mol Life Sci 2019 Feb 20. Epub 2019 Feb 20.

School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland.

The aging process is associated with chronic low-grade inflammation in both humans and rodents, commonly called inflammaging. At the same time, there is a gradual decline in the functional capacity of adaptive and innate immune systems, i.e. Read More

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http://dx.doi.org/10.1007/s00018-019-03048-xDOI Listing
February 2019

RDH1 suppresses adiposity by promoting brown adipose adaptation to fasting and re-feeding.

Cell Mol Life Sci 2019 Feb 20. Epub 2019 Feb 20.

Graduate Program in Metabolic Biology, Department of Nutritional Sciences and Toxicology, University of California, Berkeley, 119 Morgan Hall, Berkeley, CA, 94720-3104, USA.

RDH1 is one of the several enzymes that catalyze the first of the two reactions to convert retinol into all-trans-retinoic acid (atRA). Here, we show that Rdh1-null mice fed a low-fat diet gain more weight as adiposity (17% males, 13% females) than wild-type mice by 20 weeks old, despite neither consuming more calories nor decreasing activity. Glucose intolerance and insulin resistance develop following increased adiposity. Read More

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http://dx.doi.org/10.1007/s00018-019-03046-zDOI Listing
February 2019

Presynaptic NMDA receptors control nociceptive transmission at the spinal cord level in neuropathic pain.

Cell Mol Life Sci 2019 Feb 20. Epub 2019 Feb 20.

Department of Anesthesiology and Perioperative Medicine, Center for Neuroscience and Pain Research, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Unit 110, Houston, TX, 77030, USA.

Chronic neuropathic pain is a debilitating condition that remains challenging to treat. Glutamate N-methyl-D-aspartate receptor (NMDAR) antagonists have been used to treat neuropathic pain, but the exact sites of their actions have been unclear until recently. Although conventionally postsynaptic, NMDARs are also expressed presynaptically, particularly at the central terminals of primary sensory neurons, in the spinal dorsal horn. Read More

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http://dx.doi.org/10.1007/s00018-019-03047-yDOI Listing
February 2019

Expression and purification of recombinant extracellular sulfatase HSulf-2 allows deciphering of enzyme sub-domain coordinated role for the binding and 6-O-desulfation of heparan sulfate.

Cell Mol Life Sci 2019 Feb 20. Epub 2019 Feb 20.

Univ. Grenoble Alpes, CNRS, CEA, IBS, 38000 Grenoble, France.

Through their ability to edit 6-O-sulfation pattern of Heparan sulfate (HS) polysaccharides, Sulf extracellular endosulfatases have emerged as critical regulators of many biological processes, including tumor progression. However, study of Sulfs remains extremely intricate and progress in characterizing their functional and structural features has been hampered by limited access to recombinant enzyme. In this study, we unlock this critical bottleneck, by reporting an efficient expression and purification system of recombinant HSulf-2 in mammalian HEK293 cells. Read More

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http://dx.doi.org/10.1007/s00018-019-03027-2DOI Listing
February 2019

Protein S-nitrosylation in programmed cell death in plants.

Cell Mol Life Sci 2019 Feb 19. Epub 2019 Feb 19.

College of Horticulture, Gansu Agricultural University, Lanzhou, People's Republic of China.

Programmed cell death (PCD) is associated with different phases of plant life and provides resistance to different kinds of biotic or abiotic stress. The redox molecule nitric oxide (NO) is usually produced during the stress response and exerts dual effects on PCD regulation. S-nitrosylation, which NO attaches to the cysteine thiol of proteins, is a vital posttranslational modification and is considered as an essential way for NO to regulate cellular redox signaling. Read More

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http://dx.doi.org/10.1007/s00018-019-03045-0DOI Listing
February 2019

Helicobacter pylori infection and gastric cancer biology: tempering a double-edged sword.

Cell Mol Life Sci 2019 Feb 19. Epub 2019 Feb 19.

Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece.

Helicobacter pylori (H. pylori) infection affects an estimated 4.4 billion people globally. Read More

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http://link.springer.com/10.1007/s00018-019-03044-1
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http://dx.doi.org/10.1007/s00018-019-03044-1DOI Listing
February 2019
1 Read

Integrin-independent support of cancer drug resistance by tetraspanin CD151.

Cell Mol Life Sci 2019 Feb 18. Epub 2019 Feb 18.

Department of Cancer Immunology and Virology, Rm SM-520C, Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA, 02215, USA.

Tetraspanin protein CD151 has typically been studied as binding partner and functional regulator of laminin-binding integrins. However, we show here that CD151 supports anti-cancer drug resistance independent of integrins. CD151 ablation sensitized multiple tumor cell types to several anti-cancer drugs (e. Read More

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http://dx.doi.org/10.1007/s00018-019-03014-7DOI Listing
February 2019
1 Read

Functional high-throughput screening reveals miR-323a-5p and miR-342-5p as new tumor-suppressive microRNA for neuroblastoma.

Cell Mol Life Sci 2019 Feb 15. Epub 2019 Feb 15.

Group of Translational Research in Child and Adolescent Cancer, Vall d'Hebron Research Institute (VHIR)-Universitat Autònoma de Barcelona (UAB), Passeig Vall d'Hebron 119-129, Collserola Building. Lab 207, 08035, Barcelona, Spain.

Current therapies for most non-infectious diseases are directed at or affect functionality of the human translated genome, barely 2% of all genetic information. By contrast, the therapeutic potential of targeting the transcriptome, ~ 70% of the genome, remains largely unexplored. RNA therapeutics is an emerging field that widens the range of druggable targets and includes elements such as microRNA. Read More

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http://dx.doi.org/10.1007/s00018-019-03041-4DOI Listing
February 2019
1 Read

Cerebrospinal fluid biomarkers for understanding multiple aspects of Alzheimer's disease pathogenesis.

Cell Mol Life Sci 2019 Feb 15. Epub 2019 Feb 15.

Centre of Excellence in Alzheimer's Disease Research and Care, School of Medical and Health Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, Australia.

Alzheimer's disease (AD) is a multifactorial age-related brain disease. Numerous pathological events run forth in the brain leading to AD. There is an initial long, dormant phase before the clinical symptoms become evident. Read More

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http://dx.doi.org/10.1007/s00018-019-03040-5DOI Listing
February 2019
1 Read

Targeting angiogenesis in Duchenne muscular dystrophy.

Cell Mol Life Sci 2019 Feb 15. Epub 2019 Feb 15.

Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland.

Duchenne muscular dystrophy (DMD) represents one of the most devastating types of muscular dystrophies which affect boys already at early childhood. Despite the fact that the primary cause of the disease, namely the lack of functional dystrophin is known already for more than 30 years, DMD still remains an incurable disease. Thus, an enormous effort has been made during recent years to reveal novel mechanisms that could provide therapeutic targets for DMD, especially because glucocorticoids treatment acts mostly symptomatic and exerts many side effects, whereas the effectiveness of genetic approaches aiming at the restoration of functional dystrophin is under the constant debate. Read More

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http://dx.doi.org/10.1007/s00018-019-03006-7DOI Listing
February 2019

Exploring the molecular interface between hypoxia-inducible factor signalling and mitochondria.

Cell Mol Life Sci 2019 Feb 14. Epub 2019 Feb 14.

University of Cambridge, Cambridge Biomedical Campus, Cambridge, CB2 0AH, UK.

Oxygen is required for the survival of the majority of eukaryotic organisms, as it is important for many cellular processes. Eukaryotic cells utilize oxygen for the production of biochemical energy in the form of adenosine triphosphate (ATP) generated from the catabolism of carbon-rich fuels such as glucose, lipids and glutamine. The intracellular sites of oxygen consumption-coupled ATP production are the mitochondria, double-membraned organelles that provide a dynamic and multifaceted role in cell signalling and metabolism. Read More

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http://dx.doi.org/10.1007/s00018-019-03039-yDOI Listing
February 2019

Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution.

Cell Mol Life Sci 2019 Feb 14. Epub 2019 Feb 14.

Department of Biology of the Cell Nucleus, Institute of Molecular Genetics CAS, v.v.i., Vídeňská 1083, 142 00, Prague, Czech Republic.

The organization of the nuclear periphery is crucial for many nuclear functions. Nuclear lamins form dense network at the nuclear periphery and play a substantial role in chromatin organization, transcription regulation and in organization of nuclear pore complexes (NPCs). Here, we show that TPR, the protein located preferentially within the nuclear baskets of NPCs, associates with lamin B1. Read More

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http://dx.doi.org/10.1007/s00018-019-03037-0DOI Listing
February 2019

Establishment and maintenance of blood-lymph separation.

Cell Mol Life Sci 2019 Feb 13. Epub 2019 Feb 13.

Division of Cardiovascular Medicine, University of Massachusetts Medical School, The Albert Sherman Center, AS7-1047, 368 Plantation St, Worcester, MA, 01605, USA.

Hippocratic Corpus, a collection of Greek medical literature, described the functional anatomy of the lymphatic system in the fifth century B.C. Subsequent studies in cadavers and surgical patients firmly established that lymphatic vessels drain extravasated interstitial fluid, also known as lymph, into the venous system at the bilateral lymphovenous junctions. Read More

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http://dx.doi.org/10.1007/s00018-019-03042-3DOI Listing
February 2019
1 Read

Contribution of the eighth transmembrane segment to the function of the CFTR chloride channel pore.

Cell Mol Life Sci 2019 Feb 13. Epub 2019 Feb 13.

Department of Physiology and Biophysics, Dalhousie University, PO Box 15000, Halifax, NS, B3H 4R2, Canada.

Our molecular understanding of the cystic fibrosis transmembrane conductance regulator (CFTR)-the chloride channel that is mutated in cystic fibrosis-has been greatly enhanced by a number of recent atomic-level structures of the protein in different conformations. One surprising aspect of these structures was the finding that the eighth of CFTR's 12 membrane-spanning segments (TM8) appeared close to the channel pore. Although functional evidence supports a role for other TMs in forming the pore, such a role for TM8 has not previously been reported. Read More

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http://dx.doi.org/10.1007/s00018-019-03043-2DOI Listing
February 2019

The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin.

Cell Mol Life Sci 2019 Feb 12. Epub 2019 Feb 12.

Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre CAS, Institute of Parasitology, Branišovská 1160/31, 37005, České Budějovice, Czech Republic.

To successfully feed, ticks inject pharmacoactive molecules into the vertebrate host including cystatin cysteine protease inhibitors. However, the molecular and cellular events modulated by tick saliva remain largely unknown. Here, we describe and characterize a novel immunomodulatory cystatin, Iristatin, which is upregulated in the salivary glands of feeding Ixodes ricinus ticks. Read More

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http://dx.doi.org/10.1007/s00018-019-03034-3DOI Listing
February 2019

Tumor-associated macrophages: a short compendium.

Cell Mol Life Sci 2019 Feb 12. Epub 2019 Feb 12.

Lab of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, VIB, 3000, Leuven, Belgium.

Macrophages play an important role in tissue development and homeostasis. They serve as a nexus between adaptive and innate immunity, and employ considerable plasticity. In cancer, they play a pivotal role in chronic inflammation and tumor growth either by directly stimulating the proliferation of cancer cells or by producing angiogenic and lymphangiogenic factors. Read More

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http://link.springer.com/10.1007/s00018-018-2997-3
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http://dx.doi.org/10.1007/s00018-018-2997-3DOI Listing
February 2019
3 Reads

The coming-of-age of nucleocytoplasmic transport in motor neuron disease and neurodegeneration.

Authors:
Paulo A Ferreira

Cell Mol Life Sci 2019 Feb 11. Epub 2019 Feb 11.

Duke University Medical Center, DUEC 3802, 2351 Erwin Road, Durham, NC, 27710, USA.

The nuclear pore is the gatekeeper of nucleocytoplasmic transport and signaling through which a vast flux of information is continuously exchanged between the nuclear and cytoplasmic compartments to maintain cellular homeostasis. A unifying and organizing principle has recently emerged that cements the notion that several forms of amyotrophic lateral sclerosis (ALS), and growing number of other neurodegenerative diseases, co-opt the dysregulation of nucleocytoplasmic transport and that this impairment is a pathogenic driver of neurodegeneration. The understanding of shared pathomechanisms that underpin neurodegenerative diseases with impairments in nucleocytoplasmic transport and how these interface with current concepts of nucleocytoplasmic transport is bound to illuminate this fundamental biological process in a yet more physiological context. Read More

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http://dx.doi.org/10.1007/s00018-019-03029-0DOI Listing
February 2019
1 Read

N-acetylglucosaminyltransferases and nucleotide sugar transporters form multi-enzyme-multi-transporter assemblies in golgi membranes in vivo.

Cell Mol Life Sci 2019 Feb 8. Epub 2019 Feb 8.

Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 7A, 90220, Oulu, Finland.

Branching and processing of N-glycans in the medial-Golgi rely both on the transport of the donor UDP-N-acetylglucosamine (UDP-GlcNAc) to the Golgi lumen by the SLC35A3 nucleotide sugar transporter (NST) as well as on the addition of the GlcNAc residue to terminal mannoses in nascent N-glycans by several linkage-specific N-acetyl-glucosaminyltransferases (MGAT1-MGAT5). Previous data indicate that the MGATs and NSTs both form higher order assemblies in the Golgi membranes. Here, we investigate their specific and mutual interactions using high-throughput FRET- and BiFC-based interaction screens. Read More

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http://dx.doi.org/10.1007/s00018-019-03032-5DOI Listing
February 2019
1 Read

Exosomes: from carcinogenesis and metastasis to diagnosis and treatment of gastric cancer.

Cell Mol Life Sci 2019 Feb 8. Epub 2019 Feb 8.

Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Exosomes represent an important group of extracellular vesicles with a defined size between 40 and 150 nm and cup-shaped construction which have a pivotal role in elimination of intracellular debris and intercellular signaling networks. A line of evidence revealed the impact of different types of exosomes in initiation, progression, and metastasis of gastric cancer (GC). These bioactive vesicles mediate tumor and stromal communication network through modulation of cell signaling for carcinogenesis and pre-metastatic niche formation in distant organs. Read More

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http://dx.doi.org/10.1007/s00018-019-03035-2DOI Listing
February 2019

Characterization of functional subgroups among genetically identified cholinergic neurons in the pedunculopontine nucleus.

Cell Mol Life Sci 2019 Feb 8. Epub 2019 Feb 8.

Department of Physiology, University of Debrecen, Faculty of Medicine, Nagyerdei krt 98, Debrecen, 4012, Hungary.

The pedunculopontine nucleus (PPN) is a part of the reticular activating system which is composed of cholinergic, glutamatergic and GABAergic neurons. Early electrophysiological studies characterized and grouped PPN neurons based on certain functional properties (i.e. Read More

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http://dx.doi.org/10.1007/s00018-019-03025-4DOI Listing
February 2019

Tight junction proteins at the blood-brain barrier: far more than claudin-5.

Cell Mol Life Sci 2019 Feb 7. Epub 2019 Feb 7.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, 13125, Berlin, Germany.

At the blood-brain barrier (BBB), claudin (Cldn)-5 is thought to be the dominant tight junction (TJ) protein, with minor contributions from Cldn3 and -12, and occludin. However, the BBB appears ultrastructurally normal in Cldn5 knock-out mice, suggesting that further Cldns and/or TJ-associated marvel proteins (TAMPs) are involved. Microdissected human and murine brain capillaries, quickly frozen to recapitulate the in vivo situation, showed high transcript expression of Cldn5, -11, -12, and -25, and occludin, but also abundant levels of Cldn1 and -27 in man. Read More

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http://dx.doi.org/10.1007/s00018-019-03030-7DOI Listing
February 2019
2 Reads

Alternative polyadenylation coordinates embryonic development, sexual dimorphism and longitudinal growth in Xenopus tropicalis.

Cell Mol Life Sci 2019 Feb 7. Epub 2019 Feb 7.

Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, 99164-7620, USA.

RNA alternative polyadenylation contributes to the complexity of information transfer from genome to phenome, thus amplifying gene function. Here, we report the first X. tropicalis resource with 127,914 alternative polyadenylation (APA) sites derived from embryos and adults. Read More

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http://dx.doi.org/10.1007/s00018-019-03036-1DOI Listing
February 2019

IGFBP-3 interacts with NONO and SFPQ in PARP-dependent DNA damage repair in triple-negative breast cancer.

Cell Mol Life Sci 2019 Feb 6. Epub 2019 Feb 6.

Kolling Institute, Royal North Shore Hospital, The University of Sydney, St. Leonards, NSW, 2065, Australia.

Women with triple-negative breast cancer (TNBC) are generally treated by chemotherapy but their responsiveness may be blunted by DNA double-strand break (DSB) repair. We previously reported that IGFBP-3 forms nuclear complexes with EGFR and DNA-dependent protein kinase (DNA-PKcs) to modulate DSB repair by non-homologous end-joining (NHEJ) in TNBC cells. To discover IGFBP-3 binding partners involved in chemoresistance through stimulation of DSB repair, we analyzed the IGFBP-3 interactome by LC-MS/MS and confirmed interactions by coimmunoprecipitation and proximity ligation assay. Read More

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http://dx.doi.org/10.1007/s00018-019-03033-4DOI Listing
February 2019

Therapeutic potential of menstrual blood-derived endometrial stem cells in cardiac diseases.

Cell Mol Life Sci 2019 Feb 5. Epub 2019 Feb 5.

Institute of Chemistry and Biochemistry, Free University Berlin, 14195, Berlin, Germany.

Despite significant developments in medical and surgical strategies, cardiac diseases remain the leading causes of morbidity and mortality worldwide. Numerous studies involving preclinical and clinical trials have confirmed that stem cell transplantation can help improve cardiac function and regenerate damaged cardiac tissue, and stem cells isolated from bone marrow, heart tissue, adipose tissue and umbilical cord are the primary candidates for transplantation. During the past decade, menstrual blood-derived endometrial stem cells (MenSCs) have gradually become a promising alternative for stem cell-based therapy due to their comprehensive advantages, which include their ability to be periodically and non-invasively collected, their abundant source material, their ability to be regularly donated, their superior proliferative capacity and their ability to be used for autologous transplantation. Read More

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http://dx.doi.org/10.1007/s00018-019-03019-2DOI Listing
February 2019
1 Read

Role of bone marrow adipocytes in leukemia and chemotherapy challenges.

Cell Mol Life Sci 2019 Feb 4. Epub 2019 Feb 4.

Thalassemia and Hemoglobinopathy Research Center, Research Institute of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Adipose tissue (AT) is an extramedullary reservoir of normal hematopoietic stem cells (HSCs). Adipocytes prevent the production of normal HSCs via secretion of inflammatory factors, and adipocyte-derived free fatty acids may contribute to the development and progression of leukemia via providing energy for leukemic cells. In addition, adipocytes are able to metabolize and inactivate therapeutic agents, reducing the concentrations of active drugs in adipocyte-rich microenvironments. Read More

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http://dx.doi.org/10.1007/s00018-019-03031-6DOI Listing
February 2019
2 Reads

Evidence for skeletal muscle fiber type-specific expressions of mechanosensors.

Cell Mol Life Sci 2019 Jan 30. Epub 2019 Jan 30.

Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Cologne, Germany.

Mechanosensors govern muscle tissue integrity and constitute a subcellular structure known as costameres. Costameres physically link the muscle extracellular matrix to contractile and signaling 'hubs' inside muscle fibers mainly via integrins and are localized beneath sarcolemmas of muscle fibers. Costameres are the main mechanosensors converting mechanical cues into biological events. Read More

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http://dx.doi.org/10.1007/s00018-019-03026-3DOI Listing
January 2019

Non-obesogenic doses of fatty acids modulate the functionality of the circadian clock in the liver.

Cell Mol Life Sci 2019 Jan 29. Epub 2019 Jan 29.

Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100, Rehovot, Israel.

Saturated fatty acids, such as palmitate, lead to circadian disruption in cell culture. Moreover, information regarding the effects of unsaturated fatty acids on circadian parameters is scarce. We aimed at studying the effects of low doses of saturated as well as unsaturated fatty acids on circadian metabolism in vivo and at deciphering the mechanism by which fatty acids convey their effect. Read More

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http://dx.doi.org/10.1007/s00018-019-03023-6DOI Listing
January 2019

Dgcr8 knockout approaches to understand microRNA functions in vitro and in vivo.

Cell Mol Life Sci 2019 Jan 29. Epub 2019 Jan 29.

Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing, 100871, People's Republic of China.

Biologic function of the majority of microRNAs (miRNAs) is still unknown. Uncovering the function of miRNAs is hurdled by redundancy among different miRNAs. The deletion of Dgcr8 leads to the deficiency in producing all canonical miRNAs, therefore, overcoming the redundancy issue. Read More

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http://dx.doi.org/10.1007/s00018-019-03020-9DOI Listing
January 2019

Determinants of stem cell lineage differentiation toward chondrogenesis versus adipogenesis.

Cell Mol Life Sci 2019 Jan 28. Epub 2019 Jan 28.

Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, 64 Medical Center Drive, PO Box 9196, Morgantown, WV, 26506-9196, USA.

Adult stem cells, also termed as somatic stem cells, are undifferentiated cells, detected among differentiated cells in a tissue or an organ. Adult stem cells can differentiate toward lineage specific cell types of the tissue or organ in which they reside. They also have the ability to differentiate into mature cells of mesenchymal tissues, such as cartilage, fat and bone. Read More

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http://dx.doi.org/10.1007/s00018-019-03017-4DOI Listing
January 2019

The links between the gut microbiome and non-alcoholic fatty liver disease (NAFLD).

Cell Mol Life Sci 2019 Jan 25. Epub 2019 Jan 25.

Micalis Institute, INRA, UMR1319, Equipe AMIPEM, AgroParisTech, Université Paris-Saclay, Building 442, Domaine de Vilvert, 78350, Jouy-en-Josas, France.

NAFLD is currently the main cause of chronic liver disease in developed countries, and the number of NAFLD patients is growing worldwide. NAFLD often has similar symptoms to other metabolic disorders, including type 2 diabetes and obesity. Recently, the role of the gut microbiota in the pathophysiology of many diseases has been revealed. Read More

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http://dx.doi.org/10.1007/s00018-019-03011-wDOI Listing
January 2019
1 Read

The functional roles of exosomal long non-coding RNAs in cancer.

Cell Mol Life Sci 2019 Jan 25. Epub 2019 Jan 25.

Institute for Translational Medicine, Medical College of Qingdao University, Dengzhou Road 38, Qingdao, 266021, China.

Exosomes are extracellular membranous vesicles that are secreted by various cell types. Exosomes have become indispensable facilitators in the exchange of information between cells. More importantly, exosomes perform a crucial role in a variety of diseases including cancers. Read More

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http://dx.doi.org/10.1007/s00018-019-03018-3DOI Listing
January 2019
4 Reads
5.808 Impact Factor

Cellular responses to proteostasis perturbations reveal non-optimal feedback in chaperone networks.

Cell Mol Life Sci 2019 Jan 25. Epub 2019 Jan 25.

CSIR-Institute of Genomics and Integrative Biology, Delhi, 110025, India.

The proteostasis network (PN) comprises a plethora of proteins that are dedicated to aid in protein folding and maintenance; some with overlapping functions. Despite this, there are multiple pathophysiological states associated with depletion of chaperones. This is counter-intuitive, assuming cells have the ability to re-program transcriptional outputs in accordance with its proteostasic limitations. Read More

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http://link.springer.com/10.1007/s00018-019-03013-8
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http://dx.doi.org/10.1007/s00018-019-03013-8DOI Listing
January 2019
4 Reads

The HMG box transcription factor HBP1: a cell cycle inhibitor at the crossroads of cancer signaling pathways.

Cell Mol Life Sci 2019 Jan 25. Epub 2019 Jan 25.

Université Catholique de Louvain, de Duve Institute, Avenue Hippocrate 75, 1200, Brussels, Belgium.

HMG box protein 1 (HBP1) is a transcription factor and a potent cell cycle inhibitor in normal and cancer cells. HBP1 activates or represses the expression of different cell cycle genes (such as CDKN2A, CDKN1A, and CCND1) through direct DNA binding, cofactor recruitment, chromatin remodeling, or neutralization of other transcription factors. Among these are LEF1, TCF4, and MYC in the WNT/beta-catenin pathway. Read More

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http://dx.doi.org/10.1007/s00018-019-03012-9DOI Listing
January 2019
4 Reads

Link between the unfolded protein response and dysregulation of mitochondrial bioenergetics in Alzheimer's disease.

Cell Mol Life Sci 2019 Jan 25. Epub 2019 Jan 25.

Transfaculty Research Platform, Molecular and Cognitive Neuroscience, University of Basel, Basel, Switzerland.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder affecting more than 47.5 million people worldwide. Metabolic impairments are common hallmarks of AD, and amyloid-β (Aβ) peptide and hyperphosphorylated tau protein-the two foremost histopathological signs of AD-have been implicated in mitochondrial dysfunction. Read More

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http://dx.doi.org/10.1007/s00018-019-03009-4DOI Listing
January 2019
1 Read

Mitochondrial stress triggers a pro-survival response through epigenetic modifications of nuclear DNA.

Cell Mol Life Sci 2019 Jan 23. Epub 2019 Jan 23.

Instituto de Histología y Embriología de Mendoza (IHEM, Universidad Nacional de Cuyo, CONICET)-Centro Universitario UNCuyo, 5500, Mendoza, Argentina.

Mitochondrial dysfunction represents an important cellular stressor and when intense and persistent cells must unleash an adaptive response to prevent their extinction. Furthermore, mitochondria can induce nuclear transcriptional changes and DNA methylation can modulate cellular responses to stress. We hypothesized that mitochondrial dysfunction could trigger an epigenetically mediated adaptive response through a distinct DNA methylation patterning. Read More

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http://link.springer.com/10.1007/s00018-019-03008-5
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http://dx.doi.org/10.1007/s00018-019-03008-5DOI Listing
January 2019
4 Reads

The SEACIT complex is involved in the maintenance of vacuole-mitochondria contact sites and controls mitophagy.

Cell Mol Life Sci 2019 Jan 23. Epub 2019 Jan 23.

CNRS, UMR 8126, Université Paris-Sud 11, Institut Gustave Roussy, 114, rue Edouard Vaillant, 94805, Villejuif, France.

The major signaling pathway that regulates cell growth and metabolism is under the control of the target of rapamycin complex 1 (TORC1). In Saccharomyces cerevisiae the SEA complex is one of the TORC1 upstream regulators involved in amino acid sensing and autophagy. Here, we performed analysis of the expression, interactions and localization of SEA complex proteins under different conditions, varying parameters such as sugar source, nitrogen availability and growth phase. Read More

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http://link.springer.com/10.1007/s00018-019-03015-6
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http://dx.doi.org/10.1007/s00018-019-03015-6DOI Listing
January 2019
2 Reads

Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus.

Cell Mol Life Sci 2019 Jan 23. Epub 2019 Jan 23.

Viral Hepatitis Laboratory, Central Research Institute of Epidemiology, 3A Novogireevskaya Street, Moscow, 111123, Russian Federation.

Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is the major cause of viral persistence and chronic hepatitis B. CRISPR/Cas9 nucleases can specifically target HBV cccDNA for decay, but off-target effects of nucleases in the human genome limit their clinical utility. CRISPR/Cas9 systems from four different species were co-expressed in cell lines with guide RNAs targeting conserved regions of the HBV genome. Read More

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http://link.springer.com/10.1007/s00018-019-03021-8
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http://dx.doi.org/10.1007/s00018-019-03021-8DOI Listing
January 2019
9 Reads

Testicular germ cell tumor: a comprehensive review.

Cell Mol Life Sci 2019 Jan 22. Epub 2019 Jan 22.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing, 100101, China.

Testicular tumors are the most common tumors in adolescent and young men and germ cell tumors (TGCTs) account for most of all testicular cancers. Increasing incidence of TGCTs among males provides strong motivation to understand its biological and genetic basis. Gains of chromosome arm 12p and aneuploidy are nearly universal in TGCTs, but TGCTs have low point mutation rate. Read More

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http://dx.doi.org/10.1007/s00018-019-03022-7DOI Listing
January 2019
4 Reads

Critical role of UQCRC1 in embryo survival, brain ischemic tolerance and normal cognition in mice.

Cell Mol Life Sci 2019 Jan 21. Epub 2019 Jan 21.

Department of Anesthesiology, University of Virginia Health System, 1 Hospital Drive, PO Box 800710, Charlottesville, VA, 22908-0710, USA.

Ubiquinol cytochrome c reductase core protein I (UQCRC1) is a component of the complex III in the respiratory chain. Its biological functions are unknown. Here, we showed that knockout of UQCRC1 led to embryonic lethality. Read More

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http://dx.doi.org/10.1007/s00018-019-03007-6DOI Listing
January 2019
5.808 Impact Factor

A disease causing ATLASTIN 3 mutation affects multiple endoplasmic reticulum-related pathways.

Cell Mol Life Sci 2019 Jan 21. Epub 2019 Jan 21.

Leibniz Institut für Alternsforschung-Fritz Lipmann Institut, Beutenbergstr. 11, 07745, Jena, Germany.

Atlastins (ATLs) are membrane-bound GTPases involved in shaping of the endoplasmic reticulum (ER). Mutations in ATL1 and ATL3 cause spastic paraplegia and hereditary sensory neuropathy. We here show that the sensory neuropathy causing ATL3 Y192C mutation reduces the complexity of the tubular ER-network. Read More

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http://link.springer.com/10.1007/s00018-019-03010-x
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http://dx.doi.org/10.1007/s00018-019-03010-xDOI Listing
January 2019
4 Reads

A novel leptin receptor antagonist uncouples leptin's metabolic and immune functions.

Cell Mol Life Sci 2019 Jan 18. Epub 2019 Jan 18.

Faculty of Medicine and Health Sciences, VIB-UGent Center for Medical Biotechnology, Flanders Institute for Biotechnology, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium.

Leptin links body energy stores to high energy demanding processes like reproduction and immunity. Based on leptin's role in autoimmune diseases and cancer, several leptin and leptin receptor (LR) antagonists have been developed, but these intrinsically lead to unwanted weight gain. Here, we report on the uncoupling of leptin's metabolic and immune functions based on the cross talk with the epidermal growth factor receptor (EGFR). Read More

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http://dx.doi.org/10.1007/s00018-019-03004-9DOI Listing
January 2019
2 Reads

The role of endogenous tissue-type plasminogen activator in neuronal survival after ischemic stroke: friend or foe?

Cell Mol Life Sci 2019 Jan 17. Epub 2019 Jan 17.

Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, China.

Endogenous protease tissue-type plasminogen activator (tPA) has highly efficient fibrinolytic activity and its recombinant variants alteplase and tenecteplase are established as highly effective thrombolytic drugs for ischemic stroke. Endogenous tPA is constituted of five functional domains through which it interacts with a variety of substrates, binding proteins and receptors, thus having enzymatic and cytokine-like effects to act on all cell types of the brain. In the past 2 decades, numerous studies have explored the clinical relevance of endogenous tPA in neurological diseases, especially in ischemic stroke. Read More

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http://dx.doi.org/10.1007/s00018-019-03005-8DOI Listing
January 2019
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Monitoring activities of receptor tyrosine kinases using a universal adapter in genetically encoded split TEV assays.

Cell Mol Life Sci 2019 Jan 8. Epub 2019 Jan 8.

Department of Psychiatry and Psychotherapy, University Hospital, LMU Munich, Nussbaumstr. 7, 80336, Munich, Germany.

Receptor tyrosine kinases (RTKs) play key roles in various aspects of cell biology, including cell-to-cell communication, proliferation and differentiation, survival, and tissue homeostasis, and have been implicated in various diseases including cancer and neurodevelopmental disorders. Ligand-activated RTKs recruit adapter proteins through a phosphotyrosine (p-Tyr) motif that is present on the RTK and a p-Tyr-binding domain, like the Src homology 2 (SH2) domain found in adapter proteins. Notably, numerous combinations of RTK/adapter combinations exist, making it challenging to compare receptor activities in standardised assays. Read More

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http://dx.doi.org/10.1007/s00018-018-03003-2DOI Listing
January 2019
1 Read

Long non-coding RNA in stem cell pluripotency and lineage commitment: functions and evolutionary conservation.

Cell Mol Life Sci 2019 Jan 3. Epub 2019 Jan 3.

Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati-Traverso", CNR, 80131, Naples, Italy.

LncRNAs have recently emerged as new and fundamental transcriptional and post-transcriptional regulators acting at multiple levels of gene expression. Indeed, lncRNAs participate in a wide variety of stem cell and developmental processes, acting in cis and/or in trans in the nuclear and/or in the cytoplasmic compartments, and generating an intricate network of interactions with RNAs, enhancers, and chromatin-modifier complexes. Given the versatility of these molecules to operate in different subcellular compartments, via different modes of action and with different target specificity, the interest in this research field is rapidly growing. Read More

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http://link.springer.com/10.1007/s00018-018-3000-z
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http://dx.doi.org/10.1007/s00018-018-3000-zDOI Listing
January 2019
2 Reads

Potentiation of a neuronal nicotinic receptor via pseudo-agonist site.

Cell Mol Life Sci 2019 Jan 1. Epub 2019 Jan 1.

Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, MN, 55905, USA.

Neuronal nicotinic receptors containing α4 and β2 subunits assemble in two pentameric stoichiometries, (α4)(β2) and (α4)(β2), each with distinct pharmacological signatures; (α4)(β2) receptors are strongly potentiated by the drug NS9283, whereas (α4)(β2) receptors are unaffected. Despite this stoichiometry-selective pharmacology, the molecular identity of the target for NS9283 remains elusive. Here, studying (α4)(β2) receptors, we show that mutations at either the principal face of the β2 subunit or the complementary face of the α4 subunit prevent NS9283 potentiation of ACh-elicited single-channel currents, suggesting the drug targets the β2-α4 pseudo-agonist sites, the α4-α4 agonist site, or both sites. Read More

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http://link.springer.com/10.1007/s00018-018-2993-7
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http://dx.doi.org/10.1007/s00018-018-2993-7DOI Listing
January 2019
6 Reads

The dual face of glutamate: from a neurotoxin to a potential survival factor-metabolic implications in health and disease.

Cell Mol Life Sci 2019 Jan 1. Epub 2019 Jan 1.

Department of Biomedical Sciences and Public Health, School of Medicine, University "Politecnica delle Marche", Via Tronto 10/A, 60126, Ancona, Italy.

Glutamate is the major excitatory neurotransmitter in the central nervous system. Beyond this function, glutamate also plays a key role in intermediary metabolism in all organs and tissues, linking carbohydrate and amino acid metabolism via the tricarboxylic acid cycle. Under both physiological and pathological conditions, we have recently found that the ability of glutamate to fuel cell metabolism selectively relies on the activity of two main transporters: the sodium-calcium exchanger (NCX) and the sodium-dependent excitatory amino-acid transporters (EAATs). Read More

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http://dx.doi.org/10.1007/s00018-018-3002-xDOI Listing
January 2019
1 Read

Cellular senescence is associated with reorganization of the microtubule cytoskeleton.

Cell Mol Life Sci 2019 Jan 1. Epub 2019 Jan 1.

Department of Physiology, McGill University, Montreal, Canada.

Senescent cells undergo structural and functional changes that affect essentially every aspect of cell physiology. To date, the impact of senescence on the cytoskeleton is poorly understood. This study evaluated the cytoskeleton in two independent cellular models of kidney epithelium senescence. Read More

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http://dx.doi.org/10.1007/s00018-018-2999-1DOI Listing
January 2019
1 Read

Transcriptional repression of the ectodomain sheddase ADAM10 by TBX2 and potential implication for Alzheimer's disease.

Cell Mol Life Sci 2019 Jan 1. Epub 2019 Jan 1.

Department of Psychiatry and Psychotherapy, University Medical Center of the Johannes Gutenberg University Mainz, Untere Zahlbacher Strasse 8, 55131, Mainz, Germany.

Background: The ADAM10-mediated cleavage of transmembrane proteins regulates cellular processes such as proliferation or migration. Substrate cleavage by ADAM10 has also been implicated in pathological situations such as cancer or Morbus Alzheimer. Therefore, identifying endogenous molecules, which modulate the amount and consequently the activity of ADAM10, might contribute to a deeper understanding of the enzyme's role in both, physiology and pathology. Read More

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http://dx.doi.org/10.1007/s00018-018-2998-2DOI Listing
January 2019
2 Reads

Mammalian class I odorant receptors exhibit a conserved vestibular-binding pocket.

Cell Mol Life Sci 2019 Jan 1. Epub 2019 Jan 1.

Institute of Chemistry - Nice, UMR CNRS 7272, Université Côte d'Azur, Nice, France.

Odorant receptors represent the largest family of mammalian G protein-coupled receptors. Phylogenetically, they are split into two classes (I and II). By analyzing the entire subclass I odorant receptors sequences, we identified two class I-specific and highly conserved motifs. Read More

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http://link.springer.com/10.1007/s00018-018-2996-4
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http://dx.doi.org/10.1007/s00018-018-2996-4DOI Listing
January 2019
11 Reads

Multiple endocannabinoid-mediated mechanisms in the regulation of energy homeostasis in brain and peripheral tissues.

Cell Mol Life Sci 2019 Jan 1. Epub 2019 Jan 1.

German Resilience Center (DRZ) and Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 5, 55128, Mainz, Germany.

The endocannabinoid (eCB) system is widely expressed in many central and peripheral tissues, and is involved in a plethora of physiological processes. Among these, activity of the eCB system promotes energy intake and storage, which, however, under pathophysiological conditions, can favour the development of obesity and obesity-related disorders. It is proposed that eCB signalling is evolutionary beneficial for survival under periods of scarce food resources. Read More

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http://dx.doi.org/10.1007/s00018-018-2994-6DOI Listing
January 2019
3 Reads