5,654 results match your criteria Cellular Physiology And Biochemistry[Journal]


New Insights in Gene Expression Alteration as Effect of Paclitaxel Drug Resistance in Triple Negative Breast Cancer Cells.

Cell Physiol Biochem 2020 Jul;54(4):648-664

Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.

Background/aims: Triple negative breast cancer (TNBC) is a highly aggressive form of cancer which lacks targeted therapy options. Thus, TNBC patients have poor outcomes and a decreased survival rate than patients with other types of breast cancers. Due to the lack of surface receptors, TNBC needs a comprehensive investigation to provide more information regarding patient's therapy, as well as to understand the way how to counteract drug resistance mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000246DOI Listing

The Critical Role of Cell Metabolism for Essential Neutrophil Functions.

Cell Physiol Biochem 2020 Jun;54(4):629-647

Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro of Sul University, Sao Paulo, Sao Paulo, Brazil.

Neutrophils were traditionally considered as short-lived cells with abundant secretory and protein synthetic activity. Recent studies, however, indicate neutrophils are in reality a heterogeneous population of cells. Neutrophils differentiate from pluripotent stem cells in the bone marrow, and can further mature in the blood stream and can have different phenotypes in health and disease conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000245DOI Listing

Protein-Bound Polysaccharides from Fungus Disrupt the Crosstalk Between Breast Cancer Cells and Macrophages through Inhibition of Angiogenic Cytokines Production and Shifting Tumour-Associated Macrophages from the M2 to M1 Subtype.

Cell Physiol Biochem 2020 Jun;54(4):615-628

Department of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Torun, Poland.

Background/aims: The tumour microenvironment is rich in multiple cells that influence cancer development. Among them, macrophages are the most abundant immune cells, which secrete factors involved in carcinogenesis. Since protein-bound polysaccharides (PBP) from the fungus are believed to inhibit the growth of cancers, in the present study, we investigated whether these PBP influence crosstalk between triple-negative 4T1 breast cancer cells and RAW 264. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000244DOI Listing

Protein-Bound Polysaccharides from Induce RIPK1/RIPK3/MLKL-Mediated Necroptosis in ER-Positive Breast Cancer and Amelanotic Melanoma Cells.

Cell Physiol Biochem 2020 Jun;54(4):591-604

Department of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Torun, Poland.

Background/aims: The induction of necroptosis, a form of caspase-independent cell death, represents one of the most promising anticancer therapeutic modalities, as necroptosis serves as an alternative way to eliminate apoptosis-resistant tumor cells. Here, we investigated whether protein-bound polysaccharides (PBPs) derived from the fungus (CV) induce the necroptotic death pathway in breast cancer and melanoma cells.

Methods: MCF-7 and SKMel-188 cells were exposed to PBPs either alone or in combination with necrostatin-1 (Nec-1), GSK'872 or necrosulfonamide (NSA), pharmacological inhibitors of the kinases receptor-interacting protein 1 kinase (RIPK1), receptor interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL), respectively, which are involved in necroptotic processes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000242DOI Listing

Osmotic Response of Dorsal Root Ganglion Neurons Expressing Wild-Type and Mutant KCC3 Transporters.

Cell Physiol Biochem 2020 Jun;54(4):577-590

Department of Anesthesiology and Neuroscience Graduate Program, Vanderbilt University School of Medicine, Nashville, TN, USA,

Background/aims: Loss-of-Function (LOF) of the potassium chloride cotransporter 3 (KCC3) results in hereditary sensorimotor neuropathy with Agenesis of the Corpus Callosum (HSMN/ACC). Our KCC3 knockout mouse recapitulated axonal swelling and tissue vacuolization observed in autopsies of individuals with HSMN/ACC. We previously documented the first human case of a KCC3 gain-of-function (GOF) in which the patient also exhibited severe peripheral neuropathy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000241DOI Listing

Evaluation of the Diaphragm Muscle Remodeling, Inflammation, Oxidative Stress and Vascularization in Smokers: An Autopsy Study.

Cell Physiol Biochem 2020 Jun;54(4):567-576

Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil.

Background/aims: Cigarette smoking is a key factor in systemic inflammation and oxidative stress, and it has also been associated with the loss of muscle strength and an elevated risk of pulmonary diseases. Thus, this study aimed to analyze the effects of cigarette smoking on the diaphragm muscle structure of postmortem samples.

Methods: Immunohistochemical techniques were used for muscle remodeling (metalloproteinases 2 and 9), inflammation (cyclooxygenase-2), oxidative stress (8-hydroxy-2'-deoxyguanosine), and vascularization (vascular endothelial growth factor). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000240DOI Listing

Fluorometric Na Evaluation in Single Cells Using Flow Cytometry: Comparison with Flame Emission Assay.

Cell Physiol Biochem 2020 May;54(4):556-566

Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia,

Background/aims: Sodium is a key player in the fundamental cell functions. Fluorescent probes are indispensable tools for monitoring intracellular sodium levels in single living cells. Since the fluorescence of sodium-sensitive dyes in cells is significantly different from that in an aqueous solution, the fluorescence signal is calibrated in situ indirectly using ionophores for equalizing external and intracellular ion concentration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000239DOI Listing

Annexin A2-S100A10 Represents the Regulatory Component of Maxi-Cl Channel Dependent on Protein Tyrosine Dephosphorylation and Intracellular Ca²⁺.

Cell Physiol Biochem 2020 May;54(4):538-555

Division of Cell Signaling, National Institute for Physiological Sciences (NIPS), Okazaki, Japan,

Background/aims: Maxi-anion channel (Maxi-Cl) is ubiquitously expressed and involved in a number of important cell functions especially by serving as an ATP release pathway. We recently identified SLCO2A1 as its essential core component. However, the regulatory component required for the channel activation/inactivation remains unidentified. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000238DOI Listing
May 2020
2.875 Impact Factor

Multi-omics Reveal that c-Src Modulates the Mitochondrial Phosphotyrosine Proteome and Metabolism According to Nutrient Availability.

Cell Physiol Biochem 2020 May;54(4):517-537

Canada Research Chair in Mitochondrial Signaling and Physiopathology, Moncton, NB, Canada,

Background/aims: Src kinase family members, including c-Src, are involved in numerous signaling pathways and have been observed inside different cellular compartments. Notably, c-Src modulates carbohydrate and fatty acid metabolism and is involved in the metabolic rewiring of cancer cells. This kinase is found within mitochondria where it targets different proteins to impact on the organelle functions and overall metabolism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000237DOI Listing

Erratum.

Authors:

Cell Physiol Biochem 2020 ;54(3):515-516

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000236DOI Listing
January 2020

Erratum.

Authors:

Cell Physiol Biochem 2020 ;54(3):510-512

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000234DOI Listing
January 2020

Erratum.

Authors:

Cell Physiol Biochem 2020 ;54(3):508-509

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000233DOI Listing
January 2020

Status Epilepticus Dynamics Predicts Latency to Spontaneous Seizures in the Kainic Acid Model.

Cell Physiol Biochem 2020 May;54(3):493-507

Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy,

Background/aims: Status epilepticus (SE) might be followed by temporal lobe epilepsy (TLE), a common neurologic disorder characterized by spontaneous recurrent seizures (SRSs). However, the relationship between SE and TLE is still incompletely characterized. For this reason, in a model of TLE we evaluated the lesion extent and the onset of SRSs to determine if they were influenced by the SE dynamics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000232DOI Listing

Erratum.

Authors:

Cell Physiol Biochem 2020 ;54(3):513-514

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000235DOI Listing
January 2020

Calcium-Mediated Interactions Regulate the Subcellular Localization of Extracellular Signal-Regulated Kinases (ERKs).

Cell Physiol Biochem 2020 May;54(3):474-492

Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, Israel,

Background/aims: The subcellular localization of ERK1 and ERK2 (ERKs) in cells, which is important for proper signaling, may be regulated through protein-protein interactions. However, the proteins involved and the way they are regulated to affect localization is not entirely understood.

Methods: In order to identify the interacting proteins upon varying conditions, we used co-immunoprecipitation of ERK, active ERK and its binding CRS mutant. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000231DOI Listing

Molecular Pathways Leading to Induction of Cell Death and Anti-Proliferative Properties by Tacrolimus and mTOR Inhibitors in Liver Cancer Cells.

Cell Physiol Biochem 2020 May;54(3):457-473

Institute of Biomedicine of Seville (IBiS), Hospital University "Virgen del Rocío"/CSIC/University of Seville, Seville, Spain,

Background/aims: Orthotopic liver transplantation (OLT) is the recommended treatment for patients at early stages of hepatocarcinoma (HCC) with portal hypertension and/or increased bilirubinemia, but without vascular-associated diseases. Tumor recurrence, which is the main drawback for the survival of patients submitted to OLT for HCC, has been related to tumor-related variables and the immunosuppressive therapies. We have previously shown that Tacrolimus (FK506) exerts a more potent pro-apoptotic and anti-proliferative effects than the mammalian target of rapamycin (mTOR) inhibitors (Sirolimus and Everolimus) in liver cancer cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000230DOI Listing

Calbindin-D is a Novel Risk Gene for Neurodegenerative Disease.

Cell Physiol Biochem 2020 May;54(3):438-456

Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea,

Background/aims: Calcium homeostasis plays a crucial role in neuronal development and disease. Calbindin-D (CaBP-9k) acts as calcium modulators and sensors in various tissues. However, the neurobiological functions of CaBP-9k are unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000229DOI Listing

Leptin Resistance in the Ovary of Obese Mice is Associated with Profound Changes in the Transcriptome of Cumulus Cells.

Cell Physiol Biochem 2020 Apr;54(3):417-437

Institute of Animal Reproduction and Food Research of PAS, Department of Reproductive Immunology and Pathology, Olsztyn, Poland,

Background/aims: Obesity is associated with infertility, decreased ovarian performance and lipotoxicity. However, little is known about the aetiology of these reproductive impairments. Here, we hypothesise that the majority of changes in ovarian physiology in diet-induced obesity (DIO) are a consequence of transcriptional changes downstream of altered leptin signalling. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000228DOI Listing

Genistein and 17β-Estradiol Protect Hepatocytes from Fatty Degeneration by Mechanisms Involving Mitochondria, Inflammasome and Kinases Activation.

Cell Physiol Biochem 2020 Apr;54(3):401-416

Laboratory of Physiology and Experimental Surgery, Department of Translational Medicine, Università del Piemonte Orientale, Novara, Italy,

Background/aims: Oxidative stress and mitochondria dysfunction could be involved in the onset of non-alcoholic fatty liver disease (NAFLD) and in its progression to non-alcoholic steatohepatitis (NASH). Estrogens/phytoestrogens could counteract liver fat deposition with beneficial effects against NAFLD by unclear mechanisms. We aimed to analyze the protective effects elicited by genistein/estradiol in hepatocytes cultured in NAFLD-like medium on cell viability, triglycerides accumulation, mitochondrial function and oxidative stress and the role of NLRP3 inflammasome, toll like receptors 4 (TLR4), Akt and 5' AMP-activated protein kinase (AMPK)α1/2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000227DOI Listing

Fat Quality Matters: Distinct Proteomic Signatures Between Lean and Obese Cardiac Visceral Adipose Tissue Underlie its Differential Myocardial Impact.

Cell Physiol Biochem 2020 Apr;54(3):384-400

Cardiovascular Research and Development Center, Faculty of Medicine of the University of Porto, Porto, Portugal,

Background/aims: Heart failure with preserved ejection fraction (HFpEF) is recognised as an important cause of cardiovascular mortality and morbidity, accounting for approximately 50% of heart failure cases. Metabolic-related complications, such as obesity, have been associated with the pathophysiology of this complex syndrome. The anatomic proximity between cardiac visceral adipose tissue (CVAT) and the myocardium has been drawing attention due to its potential pathogenic role in cardiac diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000226DOI Listing

Mechano-Pharmacological Testing of L-Type Ca Channel Modulators via Human Vascular Celldrum Model.

Cell Physiol Biochem 2020 Apr;54(3):371-383

Institute for Neurophysiology, University Cologne, Cologne, Germany.

Background/aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology.

Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000225DOI Listing

CRISPR/Cas9 Technology in Translational Biomedicine.

Cell Physiol Biochem 2020 Apr;54(3):354-370

Institute of Translational Biomedicine St. Petersburg State University, Saint Petersburg, Russia.

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) - RNA-guided Cas9 endonuclease system has provided a fast and efficient method for precise genome editing in diverse mammalian species, including humans. The CRISPR/Cas9 technology allows generation of modifications into site-specific locations of the selected genes in one major step by carrying deletions, insertions or DNA donor-directed precise sequence modifications. Cas9 forms a nucleoprotein complex with a sequence-specific guide RNA to create double-stranded breaks in complementary DNA target. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000224DOI Listing

Matrix Metalloproteinase 13 from Satellite Cells is Required for Efficient Muscle Growth and Regeneration.

Cell Physiol Biochem 2020 Apr;54(3):333-353

Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA,

Background/aims: Cell migration and extracellular matrix remodeling underlie normal mammalian development and growth as well as pathologic tumor invasion. Skeletal muscle is no exception, where satellite cell migration replenishes nuclear content in damaged tissue and extracellular matrix reforms during regeneration. A key set of enzymes that regulate these processes are matrix metalloproteinases (MMP)s. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293591PMC

4,4'-Diisothiocyanato-2,2'-Stilbenedisulfonic Acid (DIDS) Modulates the Activity of KCNQ1/KCNE1 Channels by an Interaction with the Central Pore Region.

Cell Physiol Biochem 2020 Apr;54(2):321-332

Cellular Electrophysiology and Molecular Biology, Institute for Genetics of Heart Diseases, Department of Cardiovascular Medicine, University Hospital Münster, Münster, Germany,

Background/aims: The cardiac current IKs is carried by the KCNQ1/KCNE1-channel complex. Genetic aberrations that affect the activity of KCNQ1/KCNE1 can lead to the Long QT Syndrome 1 and 5 and, thereby, to a predisposition to sudden cardiac death. This might be prevented by pharmacological modulation of KCNQ1/KCNE1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000222DOI Listing

Preferential Killing of Tetraploid Colon Cancer Cells by Targeting the Mitotic Kinase PLK1.

Cell Physiol Biochem 2020 Apr;54(2):303-320

Department of Laboratory Medicine, Translational Cancer Research, Faculty of Medicine, Lund University, Lund, Sweden,

Background/aims: Chromosomal instability is a well-known factor in the progression of different types of cancer, including colorectal cancer. Chromosomal instability results in severely rearranged karyotypes and aneuploidy. Tetraploidy constitutes an intermediate phase during the polyploidy/aneuploidy cascade in oncogenesis, and tetraploid cells are particularly resistant to chemotherapy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000221DOI Listing

Galectin-3: A Cardiomyocyte Antiapoptotic Mediator at 24-Hour Post Myocardial Infarction.

Cell Physiol Biochem 2020 Apr;54(2):287-302

Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.

Background/aims: Galectin 3 (GAL-3) is a beta galactoside binding lectin that has different roles in normal and pathophysiological conditions. GAL-3 has been associated with heart failure and was linked to increased risk of death in a number of studies. GAL-3 was found to be up regulated in animal models of heart failure as well as myocardial infarction (MI). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000220DOI Listing

Human Adipose-Derived Pericytes: Biological Characterization and Reprogramming into Induced Pluripotent Stem Cells.

Cell Physiol Biochem 2020 Apr;54(2):271-286

Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt,

Background/aims: Pericytes (PCs) are multipotent vascular precursors that play a critical physiological role in the development and maintenance of blood vessel integrity. In this study, we aim to characterize PCs isolated from human abdominal adipose tissue and develop an integration-free induced pluripotent stem cells (iPSCs) using episomal vectors.

Methods: The ultrastructure of adipose tissue-derived PCs was determined using scanning and transmission electron microscopy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000219DOI Listing

Ca/Calmodulin Binding to STIM1 Hydrophobic Residues Facilitates Slow Ca-Dependent Inactivation of the Orai1 Channel.

Cell Physiol Biochem 2020 Mar;54(2):252-270

Membrane Transport Discovery Lab, Department of Nephrology and Hypertension, Inselspital Bern, Bern, Switzerland,

Background/aims: Store-operated Ca entry (SOCE) through plasma membrane Ca channel Orai1 is essential for many cellular processes. SOCE, activated by ER Ca store-depletion, relies on the gating function of STIM1 Orai1-activating region SOAR of the ER-anchored Ca-sensing protein STIM1. Electrophysiologically, SOCE is characterized as Ca release-activated Ca current (I). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000218DOI Listing

Mitochondrial Network and Biogenesis in Response to Short and Long-Term Exposure of Human BEAS-2B Cells to Aerosol Extracts from the Tobacco Heating System 2.2.

Cell Physiol Biochem 2020 Mar;54(2):230-251

Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland,

Background/aims: Adverse effects of cigarette smoke on health are widely known. Heating rather than combusting tobacco is one of strategies to reduce the formation of toxicants. The sensitive nature of mitochondrial dynamics makes the mitochondria an early indicator of cellular stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000216DOI Listing

ATP Synthase Subunit a Supports Permeability Transition in Yeast Lacking Dimerization Subunits and Modulates yPTP Conductance.

Cell Physiol Biochem 2020 Feb;54(2):211-229

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland,

Background/aims: Mitochondrial ATP synthase, in addition to being involved in ATP synthesis, is involved in permeability transition pore (PTP) formation, which precedes apoptosis in mammalian cells and programmed cell death in yeast. Mutations in genes encoding ATP synthase subunits cause neuromuscular disorders and have been identified in cancer samples. PTP is also involved in pathology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000215DOI Listing
February 2020
2.875 Impact Factor

p90RSK Inhibition Ameliorates TGF-β1 Signaling and Pulmonary Fibrosis by Inhibiting Smad3 Transcriptional Activity.

Cell Physiol Biochem 2020 Feb;54(2):195-210

Department of Pharmacology, Yeungnam University College of Medicine, Daegu, Republic of Korea,

Background/aims: Idiopathic pulmonary fibrosis (IPF) is a specific form of progressive and chronic interstitial lung disease of unknown cause. IPF is characterized by excessive deposition of extracellular matrix (ECM) and destructive pathological remodeling due to epithelial-to-mesenchymal transition (EMT). Eventually, lung interstitium thickens and stiffens and breathing becomes difficult. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000214DOI Listing
February 2020

Experimentally Induced Convulsive Seizures Are Modulated in Part by Zinc Ions through the Pharmacoresistant Ca2.3 Calcium Channel.

Cell Physiol Biochem 2020 Feb;54(2):180-194

Institute for Neurophysiology, University of Cologne, Köln, Germany,

Background/aims: Still in 1999 the first hints were published for the pharmacoresistant Ca2.3 calcium channel to be involved in the generation of epileptic seizures, as transcripts of alpha1E (Ca2.3) and alpha1G (Ca3. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000213DOI Listing
February 2020

Aflibercept and Ranibizumab Modulate Retinal Pigment Epithelial Cells Function by Acting on Their Cross Talk with Vascular Endothelial Cells.

Cell Physiol Biochem 2020 Feb;54(2):161-179

Ophthalmology Unit, Department of Health Sciences, University East Piedmont, Azienda Ospedaliera Universitaria Maggiore della Carità, Novara, Italy,

Background/aims: We performed co-culture experiments between human RPE cells (ARPE-19) and human umbilical vascular endothelial cells (HUVEC) in order to evaluate how anti-VEGF drugs could affect NO release, mitochondrial function, the oxidative status, proliferation and migration of RPE cells through modulation of their cross talk with vascular endothelial cells.

Methods: The co-culture HUVEC/RPE, was exposed to Ranibizumab/Aflibercept in the absence/presence of the NO synthase (NOS) inhibitor, the phosphatidylinositol 3'-kinase (PI3K), the extracellular-signal-regulated kinases 1/2 (ERK1/2) and the p38 mitogen-activated protein kinase (p38 MAPK) blockers. Specific kits were used for cell viability, mitochondrial membrane potential, NO, ROS and GSH production. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000212DOI Listing
February 2020

Erratum.

Authors:

Cell Physiol Biochem 2020 02;54(1):160

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000211DOI Listing
February 2020

New Nrf2-Inducer Compound ITH12674 Slows the Progression of Retinitis Pigmentosa in the Mouse Model rd10.

Cell Physiol Biochem 2020 Feb;54(1):142-159

Departamento de Óptica, Farmacología y Anatomía, Universidad de Alicante, Alicante, Spain,

Background/aims: It is well established that oxidative stress and inflammation are common pathogenic features of retinal degenerative diseases. ITH12674 is a novel compound that induces the transcription factor Nrf2; in so doing, the molecule exhibits anti-inflammatory, and antioxidant properties, and affords neuroprotection in rat cortical neurons subjected to oxidative stress. We here tested the hypothesis that ITH12674 could slow the retinal degeneration that causes blindness in rd10 mice, a model of retinitis pigmentosa. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000210DOI Listing
February 2020

Serotonin Modulates AhR Activation by Interfering with CYP1A1-Mediated Clearance of AhR Ligands.

Cell Physiol Biochem 2020 Feb;54(1):126-141

Division of Gastroenterology & Hepatology, University of Illinois at Chicago, Chicago, IL, USA,

Background/aims: Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter and hormone with important physiological functions in many organs, including the intestine. We have previously shown that 5-HT activates the aryl hydrocarbon receptor (AhR) in intestinal epithelial cells (IECs) via a serotonin transporter (SERT)-dependent mechanism. AhR is a nuclear receptor that binds a variety of molecules including tryptophan (TRP) metabolites to regulate physiological processes in the intestine including xenobiotic detoxification and immune modulation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050772PMC
February 2020

Inhibition of Sphingolipid Synthesis as a Phenotype-Modifying Therapy in Cystic Fibrosis.

Cell Physiol Biochem 2020 01;54(1):110-125

Biochemistry and Molecular Biology Laboratory, Health Sciences Department, University of Milan, Italy,

Background/aims: Cystic Fibrosis (CF) is an inherited disease associated with a variety of mutations affecting the CFTR gene. A deletion of phenylalanine 508 (F508) affects more than 70% of patients and results in unfolded proteins accumulation, originating a proteinopathy responsible for inflammation, impaired trafficking, altered metabolism, cholesterol and lipids accumulation, impaired autophagy at the cellular level. Lung inflammation has been extensively related to the accumulation of the lipotoxin ceramide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000208DOI Listing
January 2020

Extracellular Vesicles: Cell-Derived Biomarkers of Glomerular and Tubular Injury.

Cell Physiol Biochem 2020 Jan;54(1):88-109

Kidney Research Centre, Department of Cellular and Molecular Medicine, The Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada,

Extracellular vesicles (EVs) are important mediators of intercellular communication. Since EVs are also released during pathological conditions, there has been considerable interest in their potential as sensitive biomarkers of cellular stress and/or injury. In the context of kidney disease, urinary EVs are promising indicators of glomerular and tubular damage. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000207DOI Listing
January 2020

Integrin Linked Kinase (ILK) Downregulation as an Early Event During the Development of Metabolic Alterations in a Short-Term High Fat Diet Mice Model.

Cell Physiol Biochem 2020 Jan;54(1):71-87

Department of Systems Biology, Physiology Unit, from Universidad de Alcalá, Alcalá de Henares, Madrid, Spain,

Background/aims: Diabetes type 2, metabolic syndrome or non-alcoholic fatty liver disease are insulin resistance-related metabolic disorders, which lack a better prognosis before their full establishment. We studied the importance of the intracellular scaffold protein integrin linked kinaes (ILK) as a key modulator in the initial pathogenesis and the early progression of those insulin resistance- related disorders.

Methods: Adult mice with a global transgenic downregulation of ILK expression (cKD-ILK) and littermates without that depletion (CT) were fed with either standard (STD) or high fat (HFD) diets during 2 and 6 weeks. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000206DOI Listing
January 2020

Genistein Represses HOTAIR/Chromatin Remodeling Pathways to Suppress Kidney Cancer.

Cell Physiol Biochem 2020 01;54(1):53-70

Department of Urology, San Francisco Veterans Affairs Medical Center and University of California San Francisco, San Francisco, USA,

Background/aims: Genistein, a soy isoflavone, has been shown to have anti-cancer effects in various cancers including renal cancer. Long non-coding RNA, HOX transcript antisense RNA (HOTAIR), is involved in cancer progression and metastasis, such as renal cancer. Our aim was to investigate the effects of genistein on HOTAIR chromatin remodeling functions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000205DOI Listing
January 2020

Autoimmune Regulator Enhanced the Expression of Caspase-3 and Did Not Induce Massive Germ Cell Apoptosis in GC1-Spg Cells.

Cell Physiol Biochem 2020 Jan;54(1):40-52

Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology, Thycaud, Poojappura, Kerala, India,

Background/aims: AIRE is known for its involvement in autoreactive T-cell deletion in thymic epithelium. Though extrathymic expression of AIRE is well documented, the functional relevance of AIRE in non-thymus tissues is emerging. AIRE is expressed in neonatal and adult testis, and has been implicated in sporadic germ cell apoptosis in developing testis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000204DOI Listing
January 2020
2.875 Impact Factor

CaMKII Modulates the Cardiac Transient Outward K Current through its Association with Kv4 Channels in Non-Caveolar Membrane Rafts.

Cell Physiol Biochem 2020 Jan;54(1):27-39

Department of Physiology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Vitoria, Spain.

Background/aims: To test whether the physiological regulation of the cardiac Kv4 channels by the Ca/calmodulin-dependent protein kinase II (CaMKII) is restricted to lipid rafts and whether the interactions observed in rat cardiomyocytes also occur in the human ventricle.

Methods: Ventricular myocytes were freshly isolated from Sprague-Dawley rats. I was recorded by the whole-cell Patch-Clamp technique. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000203DOI Listing
January 2020

Nanoscale Mapping Reveals Functional Differences in Ion Channels Populating the Membrane of Primary Cilia.

Cell Physiol Biochem 2020 Jan;54(1):15-26

School of Engineering and Materials Science, Queen Mary University of London, London, UK,

Background/aims: The primary cilium is a nanoscale membrane protrusion believed to act as a mechano-chemical sensor in a range of different cell types. Disruptions in its structure and signalling have been linked to a number of medical conditions, referred to as ciliopathies, but remain poorly understood due to lack of techniques capable of investigating signal transduction in cilia at nanoscale. Here we set out to use latest advances in nanopipette technology to address the question of ion channel distribution along the structure of primary cilium. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000202DOI Listing
January 2020

YOD1 Deubiquitinates NEDD4 Involved in the Hippo Signaling Pathway.

Cell Physiol Biochem 2020 Jan;54(1):1-14

Department of Biomedical Science, CHA University, Gyeonggi, Republic of Korea,

Background/aims: Deubiquitinating enzymes (DUBs) are crucially involved in controlling signal transductions, and reverse ubiquitination by removing the ubiquitin from protein substrates. The Hippo signaling has an important role in tissue growth, cell proliferation, differentiation, and apoptosis. Since disruption of the Hippo signaling is associated with a number of diseases, it is imperative to investigate the molecular mechanism of the Hippo signaling. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000201DOI Listing
January 2020

Control of Muscle Fibro-Adipogenic Progenitors by Myogenic Lineage is Altered in Aging and Duchenne Muscular Dystrophy.

Cell Physiol Biochem 2019 ;53(6):1029-1045

Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France.

Background/aims: Fibro-adipogenic progenitors (FAPs), a muscle-resident stem cell population, have recently emerged as important actors of muscle regeneration by interacting with myogenic progenitors (MPs) to promote the formation of new muscle fibers. However, FAPs are also considered as main contributors of intramuscular fibrotic and fat depositions, resulting in a poor quality of muscles and a defective regeneration in aging and Duchenne Muscular Dystrophy disease (DMD). Therefore, the understanding of the control of FAP fate is an important aspect of muscle repair and homeostasis, but little is known in humans. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000196DOI Listing
January 2020

The Contribution of Mitochondrial Ion Channels to Cancer Development and Progression.

Cell Physiol Biochem 2019 ;53(S1):63-78

Department of Biology, University of Padova, Padova, Italy,

Mitochondria play a central role in cancer development, by contributing to most of the classical hallmarks of cancer, including sustained proliferation, metabolic re-programming, apoptosis resistance, invasion and induction of angiogenesis [1]. In addition, mitochondria affect also the function of anti- and pro-tumoral immune cells in the tumor microenvironment. Mitochondria harbor a plethora of regulated ion channels whose function is related to ion/ metabolite transport and to fine-tuning of mitochondrial membrane potential as well as of reactive oxygen species release. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000198DOI Listing
January 2020

Mitochondrial Kv1.3: a New Target in Cancer Biology?

Cell Physiol Biochem 2019 ;53(S1):52-62

Department of Biology, University of Padova, Padova, Italy,

Kv1.3 is a voltage gated potassium channel located in the plasma membrane, as well as at intracellular levels, such as mitochondria (mitoKv1.3), nucleus and Golgi apparatus. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000195DOI Listing
December 2019

Clinical Development of Sphingosine as Anti-Bacterial Drug: Inhalation of Sphingosine in Mini Pigs has no Adverse Side Effects.

Cell Physiol Biochem 2019 ;53(6):1015-1028

Department of Molecular Biology, University Clinic, University of Duisburg-Essen, Essen, Germany,

Background/aims: Pulmonary infections with Pseudomonas aeruginosa (P. aeruginosa) or Staphylococcus aureus (S. aureus) are of utmost clinical relevance in patients with cystic fibrosis, chronic obstructive pulmonary disease, after trauma and burn, upon ventilation or in immuno-compromised patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000194DOI Listing
December 2019

Schlafen12 Reduces the Aggressiveness of Triple Negative Breast Cancer through Post-Transcriptional Regulation of ZEB1 That Drives Stem Cell Differentiation.

Cell Physiol Biochem 2019 ;53(6):999-1014

Department of Biomedical Sciences, University of North Dakota School of Medicine and the Health Sciences, Grand Forks, ND, USA,

Background/aims: Schlafen12 (SLFN12) promotes human intestinal and prostatic epithelial differentiation. We sought to determine whether SLFN12 reduces triple-negative breast cancer (TNBC) aggressiveness.

Methods: We validated bioinformatics analyses of publicly available databases by staining human TNBC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007982PMC
December 2019
2.875 Impact Factor