5,498,090 results match your criteria Cellular Immunology [Journal]

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Treatment of male stress urinary incontinence using autologous adipose-derived regenerative cells: Long-term efficacy and safety.

Int J Urol 2018 Dec 17. Epub 2018 Dec 17.

Department of Plastic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan.

Objectives: To investigate the long-term efficacy and safety of periurethral injection of autologous adipose-derived regenerative cells for the treatment of post-prostatectomy stress urinary incontinence.

Methods: A total of 13 patients with persistent stress urinary incontinence after prostate surgery (radical prostatectomy, 10 patients; holmium laser enucleation of the prostate, three patients) underwent periurethral injection of adipose-derived regenerative cells and were followed up for >4 years. A 24-h pad test was carried out for four consecutive days in each evaluation period, and changes in the mean daily leakage volume during the 4 days from baseline to 60 months after treatment were evaluated. Read More

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December 2018

Apolipoprotein CIII and diabetes. Is there a link?

Diabetes Metab Res Rev 2018 Dec 17:e3118. Epub 2018 Dec 17.

Department of Internal Medicine, School of Medicine, University of Ioannina, Ioannina, Greece.

Apolipoprotein CIII (ApoCIII), a small protein that resides on the surface of lipoprotein particles, is a key regulator of triglyceride metabolism. The inhibition of lipoprotein lipase (LPL), the increased assembly and secretion of very low-density lipoproteins (VLDL) and the decreased reuptake of triglyceride-rich lipoproteins (TRLs) by the liver are mechanisms associating elevated serum ApoCIII levels and hypertriglyceridemia. ApoCIII concentration is high in individuals with diabetes mellitus indicating a possible positive correlation with impairment of glucose metabolism. Read More

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December 2018

Primary Human Neutrophils Exhibit a Unique HIV-Directed Antibody-Dependent Phagocytosis Profile.

J Innate Immun 2018 Dec 17:1-10. Epub 2018 Dec 17.

The only clinical HIV vaccine trial to demonstrate efficacy, RV144, correlated protection with the antibodies (Abs) mediating function via the "constant" immunoglobulin region, the crystallizable fragment (Fc). These data have supported a focus on the induction of Abs by vaccines that trigger antiviral activities by relevant leukocytes via Fc receptors (FcRs). Neutrophils are phagocytes that comprise > 50% of leukocytes and display unique FcRs. Read More

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December 2018
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Towards Understanding the Molecular Basis of Nitric Oxide-Regulated Group Behaviors in Pathogenic Bacteria.

J Innate Immun 2018 Dec 17:1-11. Epub 2018 Dec 17.

Pathogenic bacteria have many strategies for causing disease in humans. One such strategy is the ability to live both as single-celled motile organisms or as part of a community of bacteria called a biofilm. Biofilms are frequently adhered to biotic or abiotic surfaces and are extremely antibiotic resistant. Read More

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December 2018

Downregulation of Microparticle Release and Pro-Inflammatory Properties of Activated Human Polymorphonuclear Neutrophils by LMW Fucoidan.

J Innate Immun 2018 Dec 17:1-17. Epub 2018 Dec 17.

Laboratório de Farmacologia Celular e Molecular, Universidade do Estado do Rio de Janeiro, Rio de Janeiro,

Exposition of neutrophils (polymorphonuclear neutrophils, PMNs) to bacterial products triggers exacerbated activation of these cells, increasing their harmful effects on host tissues. We evaluated the possibility of interfering with the classic immune innate responses of human PMNs exposed to bacterial endotoxin (lipopolysaccharide, LPS), and further stimulated with bacterial formyl peptide (N-formyl-methionine-leucine-phenylalanine, fMLP). We showed that the low- molecular-weight fucoidan (LMW-Fuc), a polysaccharide extracted from brown algae, attenuated the exacerbated activation induced by fMLP on LPS-primed PMNs, in vitro, impairing chemotaxis, NET formation, and the pro-survival and pro-oxidative effects. Read More

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December 2018

Testing the gene expression classification of the EMT spectrum.

Phys Biol 2018 Dec 17. Epub 2018 Dec 17.

Department of Bioengineering, Rice University, 6100 Main Street, MS-142, Houston, TX 77251-1892, USA, Houston, Texas, UNITED STATES.

The epithelial-mesenchymal transition (EMT) plays a central role in cancer metastasis and drug resistance - two persistent clinical challenges. Epithelial cells can undergo a partial or full EMT, attaining either a hybrid epithelial/mesenchymal (E/M) or mesenchymal phenotype, respectively. Recent studies have emphasized that hybrid E/M cells may be more aggressive than their mesenchymal counterparts. Read More

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December 2018

Fluid distribution and cell integrity indicators evaluated by bioelectrical impedance in university athletes: comparison between team sports and individual sports.

Physiol Meas 2018 Dec 17. Epub 2018 Dec 17.

Research Center in Kinanthropometry and Human Performance, Federal University of Santa Catarina, Florianopolis, BRAZIL.

The objective was to compare indicators of fluid distribution and cellular integrity in university athletes. Cross-sectional study with 167 university athletes (18-35 years), of team and individual sports. The bioimpedance was used to measure body water (TBW), intracellular water (ICW), extracellular water (ECW), ECW/ICW, impedance (Z), reactance (Xc), resistance (R), phase angle, body cell mass (BCM) and ECW/BCM at frequency 50khz. Read More

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December 2018

Controllable fabrication of hydroxybutyl chitosan/oxidized chondroitin sulfate hydrogels by 3D bioprinting technique for cartilage tissue engineering.

Biomed Mater 2018 Dec 17. Epub 2018 Dec 17.

Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, CHINA.

Biological regeneration of articular cartilage continues to be a challenge at present. Functional engineered implants with patient-specific sizes are difficult to achieve. The aim of this study is to fabricate a biocompatible cell-laden hydrogel with a designable structure. Read More

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December 2018

miR-505-3p is a repressor of the puberty onset in female mice.

J Endocrinol 2018 Dec 1. Epub 2018 Dec 1.

J Xiao, The College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.

Puberty onset is a complex trait regulated by multiple genetic and environmental factors. In this study, we narrowed a puberty related QTL region down to a 1.7 Mb region on chromosome X in female mice and inferred miR-505-3p as the functional gene. Read More

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December 2018

Non-canonical AR activity facilitates endocrine resistance in breast cancer.

Endocr Relat Cancer 2019 Feb;26(2):251-264

Garvan Institute of Medical Research, Darlinghurst, New South Wales, Australia.

The role of androgen receptor (AR) in endocrine-resistant breast cancer is controversial and clinical trials targeting AR with an AR antagonist (e.g., enzalutamide) have been initiated. Read More

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February 2019
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An insight into DNA binding properties of newly designed cationic δ,δ'‑diazacarbazoles: Spectroscopy, AFM imaging and living cells staining studies.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Dec 10;211:260-271. Epub 2018 Dec 10.

CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, PR China. Electronic address:

Two cationic δ,δ'‑diazacarbazoles, 1‑Methyl‑5H‑pyrrolo[3,2‑b:4,5‑b']dipyridinium iodide (MPDPI) and 1,5‑Dimethyl‑5H‑pyrrolo[3,2‑b:4,5‑b']dipyridinium iodide (DPDPI), were devised and synthesized. Through characterizations of the interactions between DNA and the two δ,δ'‑diazacarbazoles by various spectroscopy means, the strong interactions between the two compounds and double-strand DNA have been observed and the interaction types and mechanisms were explored. UV-Vis and fluorescent data have shown the big changes of DNA in the presence of either of the two compounds, demonstrating that both of the δ,δ'‑diazacarbazoles can bind to DNA tightly, and high ionic strength decreased the intercalative interactions. Read More

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December 2018

Regulation of inositol 1,4,5-trisphosphate-induced Ca release from the endoplasmic reticulum by AMP-activated kinase modulators.

Cell Calcium 2018 Dec 11;77:68-76. Epub 2018 Dec 11.

Institute of Biology and Molecular Genetics (IBGM), Department of Biochemistry and Molecular Biology and Physiology, Faculty of Medicine, University of Valladolid and CSIC, Ramón y Cajal, 7, E-47005 Valladolid, Spain. Electronic address:

The 5' AMP-activated protein kinase (AMPK) is a nutrient-sensitive kinase that plays a key role in the control of cellular energy metabolism. We have explored here the relationship between AMPK and Ca signaling by looking at the effect of an AMPK activator (A769662) and an AMPK inhibitor (dorsomorphin) on histamine-induced Ca-release from the endoplasmic reticulum (ER) in HeLa cells. Our data show that incubation with A769662 (EC = 29 μM) inhibited histamine-induced Ca-release from the ER in intact cells, as well as inositol-1,4,5-trisphosphate (IP)-induced Ca release in permeabilized cells. Read More

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December 2018

Current antifungal drugs and immunotherapeutic approaches as promising strategies to treatment of fungal diseases.

Biomed Pharmacother 2018 Dec 14;110:857-868. Epub 2018 Dec 14.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Invasive candidiasis (IC) can affect individuals with various underlying diseases hospitalized in different parts of hospitals. In recent decades, IC has caused 27-55% mortality in general population. Although Candida albicans (C. Read More

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December 2018

MiR-542-3p, a microRNA targeting CDK14, suppresses cell proliferation, invasiveness, and tumorigenesis of epithelial ovarian cancer.

Biomed Pharmacother 2018 Dec 14;110:850-856. Epub 2018 Dec 14.

Department of Gynaecology and Obstetrics, Jinshan Hospital affiliated to Fudan University, Shanghai 201508, China. Electronic address:

MicroRNA-542-3p (miR-542-3p) has been implicated in several cancers; however, its precise role in ovarian cancer is unclear. In this study, we found that miR-542-3p was significantly downregulated in epithelial ovarian cancer (EOC) tissues and cell lines. Functional assays showed that overexpression of miR-542-3p suppressed tumor cell proliferation, migration, and invasion in vitro, whereas miR-542-3p knockdown dramatically promoted tumor cell proliferation and invasion. Read More

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December 2018

Rapamycin ameliorates lipopolysaccharide-induced acute lung injury by inhibiting IL-1β and IL-18 production.

Int Immunopharmacol 2018 Dec 14;67:211-219. Epub 2018 Dec 14.

Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China; Department of Pulmonary and Critical Care Medicine, Center for Respiratory Diseases, China-Japan Friendship Hospital, Beijing 100029, China; National Clinical Research Center for Respiratory Diseases, Beijing 100029, China; Department of Respiratory Medicine, Capital Medical University, Beijing 100069, China; Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China. Electronic address:

Interleukin (IL)-1β and IL-18 play central and detrimental roles in the development of acute lung injury (ALI), and mammalian target of rapamycin (mTOR) is involved in regulating IL-1β and IL-18 production. However, it is not clear whether the mTOR specific inhibitor rapamycin can attenuate lipopolysaccharide (LPS)-induced ALI by modulating IL-1β and IL-18 production. In this study, we found that rapamycin ameliorated LPS-induced ALI by inhibiting NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-mediated IL-1β and IL-18 secretion. Read More

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December 2018

Interleukin-7 promotes lung-resident CD14 monocytes activity in patients with lung squamous carcinoma.

Int Immunopharmacol 2018 Dec 14;67:202-210. Epub 2018 Dec 14.

Department of Radiation Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China. Electronic address:

Interleukin (IL)-7 enhances cytokines secretion by CD14 monocytes, and induces recruitment of monocytes to endothelium. As an important regulator to different types of immune cells, the role of IL-7 in modulation of CD14 monocytes is still not fully elucidated. Thus, the aim of current study was to investigate the immunoregulatory activity of IL-7 to peripheral and lung-resident CD14 monocytes in lung squamous carcinoma patients. Read More

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December 2018

Donor T-cell-derived interleukin-22 promotes thymus regeneration and alleviates chronic graft-versus-host disease in murine allogeneic hematopoietic cell transplant.

Int Immunopharmacol 2018 Dec 14;67:194-201. Epub 2018 Dec 14.

Blood Diseases Institute, Xuzhou Medical University, Xuzhou, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, China. Electronic address:

Defect of thymus results in poor posttransplant immune recovery and dysfunction of immune tolerance after allogeneic hematopoietic cell transplants (allo-HCT). Improving thymus regeneration represents a potential strategy to accelerate recovery of T-cell immunity. IL-22 was reported to mediate thymus regeneration after injury. Read More

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December 2018
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Urinary metals and leukocyte telomere length in American Indian communities: The Strong Heart and the Strong Heart Family Study.

Environ Pollut 2018 Dec 5;246:311-318. Epub 2018 Dec 5.

Area of Cardiometabolic and Renal Risk, Biomedical Research Institute Hospital Clinic of Valencia (INCLIVA), Valencia, Spain; Department of Environmental Health and Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Chronic Diseases Epidemiology, National Center for Epidemiology, Carlos III Health Institutes, Madrid, Spain.

Introduction: While several mechanisms may explain metal-related health effects, the exact cellular processes are not fully understood. We evaluated the association between leukocyte telomere length (LTL) and urine arsenic (ΣAs), cadmium (Cd) and tungsten (W) exposure in the Strong Heart Study (SHS, N = 1702) and in the Strong Heart Family Study (SHFS, N = 1793).

Methods: Urine metal concentrations were measured using ICP-MS. Read More

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December 2018

The facile synthesis of copper oxide quantum dots on chitosan with assistance of phyto-angelica for enhancing the human osteoblast activity to the application of osteoporosis.

J Photochem Photobiol B 2018 Nov 14;191:6-12. Epub 2018 Nov 14.

Department of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, 156 Yucai Road, Hangzhou, Zhejiang 311201, PR China.

Osteoblasts are an important key factor for the pathogenesis of several bone-related diseases, notably in osteoporosis. Osteoporosis is a disorder categorized based on the bone mineral density (BMD) and an alteration in the bone micro-architecture had been considered as the major determinant for increasing the fracture risk. The available medicine for curing the osteoporosis shows a minimal or no activity against the genesis or function of osteoblasts. Read More

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November 2018

Antibacterial and photocatalytic activity of hydrothermally synthesized SnO doped GO and CNT under visible light irradiation.

J Photochem Photobiol B 2018 Dec 12;191:18-25. Epub 2018 Dec 12.

Department of Civil Engineering, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea. Electronic address:

Bacterial and dye pollution are major problems with wastewater treatment. An increasing number of photocatalysts are being used in industry to kill bacterial and reduce pollution. In the present study, highly stable SnO-doped nanocomposites have been prepared successively by a hydrothermal method. Read More

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December 2018

Biosynthesis of sorafenib coated graphene nanosheets for the treatment of gastric cancer in patients in nursing care.

J Photochem Photobiol B 2018 Nov 20;191:1-5. Epub 2018 Nov 20.

The Third Department of Tumor Surgery, Tangshan Gongren Hospital, People's Republic of China. Electronic address:

Sorafenib (SRF) is a well-known tyrosine kinase inhibiting anticancer drug which iseffectual against multiple carcinomas especially gastric cancers by targeting the Ras/Raf/Mek/Erk cascade pathway and blocking the tumor cell proliferation. In the present work, we have reduced graphene oxide (GO) in presence of sorafenib using ascorbic as green reducing agent for the treatment of gastric cancers. Sorafenib reduced graphene oxide (SRGO) were obtained with a transparent and smoothmorphology. Read More

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November 2018

Flux modeling for monolignol biosynthesis.

Curr Opin Biotechnol 2018 Dec 14;56:187-192. Epub 2018 Dec 14.

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China; Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, United States. Electronic address:

The pathway of monolignol biosynthesis involves many components interacting in a metabolic grid to regulate the supply and ratios of monolignols for lignification. The complexity of the pathway challenges any intuitive prediction of the output without mathematical modeling. Several models have been presented to quantify the metabolic flux for monolignol biosynthesis and the regulation of lignin content, composition, and structure in plant cell walls. Read More

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December 2018

Extracellular polymeric substances of biofilms: Suffering from an identity crisis.

Water Res 2018 Nov 28;151:1-7. Epub 2018 Nov 28.

Department of Biotechcnology, Delft University of Technology, Delft, the Netherlands. Electronic address:

Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Read More

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November 2018

Chronic stress exposure and daily stress appraisals relate to biological aging marker p16.

Psychoneuroendocrinology 2018 Dec 7;102:139-148. Epub 2018 Dec 7.

Cousins Center for Psychoneuroimmunology, Semel Institute for Neuroscience and Human Behavior, University of California, 300 Medical Plaza, Los Angeles, CA 90095, USA; Department of Psychology, University of California, 502 Portola Plaza, Los Angeles, CA 90095, USA. Electronic address:

Previous research has linked exposure to adverse social conditions with DNA damage and accelerated telomere shortening, raising the possibility that chronic stress may impact biological aging pathways, ultimately increasing risk for age-related diseases. Less clear, however, is whether these stress-related effects extend to additional hallmarks of biological aging, including cellular senescence, a stable state of cell cycle arrest. The present study aimed to investigate associations between psychosocial stress and two markers of cellular aging-leukocyte telomere length (LTL) and cellular senescence signal p16. Read More

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December 2018
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Oxidative damage induced by copper in testis of the red swamp crayfish Procambarus clarkii and its underlying mechanisms.

Aquat Toxicol 2018 Dec 6;207:120-131. Epub 2018 Dec 6.

Ecological Society of Shandong, Zhijinshi Jie 12, Jinan 250012, China; Shandong Institute of Environmental Science, Lishan Lu 50, Jinan 250013, China. Electronic address:

Copper (Cu) is one of the most widespread environmental pollutants and is known to exert multiple toxic effects including reproductive toxicity. In this study, we investigated the toxic effect of Cu on reproduction of the red swamp crayfish (Procambarus clarkii), an economic crustacean species, by exposing adult male crayfish to 0.03 and 3. Read More

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December 2018

Pruritus is Common and Under-treated in Patients with Primary Biliary Cholangitis in the United Kingdom.

Clin Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.

Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom; NIHR Newcastle Biomedical Research Centre, Newcastle University, Newcastle upon Tyne, United Kingdom.

Background And Aims: Little is known about the prevalence or treatment of pruritus associated with primary biliary cholangitis (PBC). We analyzed data from patients with PBC recruited from all clinical centers in the United Kingdom (UK) to characterize the prevalence, severity, progression, and treatment of pruritus.

Methods: We performed cross-sectional and longitudinal study of patients in the UK-PBC cohort to assess trajectories of pruritus. Read More

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December 2018

Characterizing the effect of Poast on Chlorella vulgaris, a non-target organism.

Chemosphere 2018 Dec 8;219:704-712. Epub 2018 Dec 8.

Marshall University Department of Chemistry, One John Marshall Dr., Huntington, WV, 25755, USA. Electronic address:

Herbicides may cause unexpected damage to non-target organisms as it is challenging to predict undesirable biotic interactions. Poast is a widely used herbicide formulation that contains sethoxydim and targets the acetyl-CoA carboxylase of perennial grasses. In this study, Chlorella vulgaris, a unicellular green microalga, was exposed to a 0. Read More

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December 2018

Differentiation of Common Variable Immunodeficiency from Igg Deficiency.

J Allergy Clin Immunol Pract 2018 Dec 14. Epub 2018 Dec 14.

Division of Clinical Immunology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York. Electronic address:

Background: Common variable immunodeficiency (CVID) and IgG deficiency are two of the more prevalent primary humoral immune defects. Whereas the former is defined by consensus with criteria for quantitative and qualitative antibody defects, the latter is used to describe patients with reduced IgG, who commonly have recurrent sinopulmonary infections but do not fulfill CVID criteria. However, these patients are often given this diagnosis. Read More

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December 2018

Profiling of miRNAs in serum of children with attention-deficit hyperactivity disorder shows significant alterations.

J Psychiatr Res 2018 Dec 8;109:185-192. Epub 2018 Dec 8.

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran; CEINGE-biotecnologie Avanzate, Naples, Italy. Electronic address:

Background: Attention-deficit/hyperactivity disorder (ADHD), a common psychiatric disorder, is identified by abnormal levels of impulsivity, inattention, and hyperactivity. MiRNAs play important roles in neural network development of the brain. Circulating miRNAs (cmiRNAs) are offered as promising noninvasive markers for psychiatric disorders. Read More

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December 2018

The Intestinal Lymphatic System: Functions and Metabolic Implications.

Cell Mol Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.

Cardiovascular Research Center and Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510-3221, USA; INSERM U970, Paris Cardiovascular Research Center, 75015 Paris, France.

The lymphatic system of the gut plays important roles in the transport of dietary lipids, as well as in immunosurveillance and removal of interstitial fluid. Historically, despite its crucial functions in intestinal homeostasis, the lymphatic system has been poorly studied. In the last two decades, identification of specific molecular mediators of lymphatic endothelial cells (LECs) growth together with novel genetic approaches and intravital imaging techniques, have advanced our understanding of the mechanisms regulating intestinal lymphatic physiology in health and disease. Read More

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December 2018
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Ligation of CD180 contributes to endotoxic shock by regulating the accumulation and immunosuppressive activity of myeloid-derived suppressor cells through STAT3.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address:

Myeloid-derived suppressor cells (MDSCs) play an immunosuppressive role in the pathogenesis of inflammatory diseases. CD180, a TLR-like protein, can regulate the proliferation and activation of immune cells. However, the roles of CD180 in regulating the accumulation and function of MDSCs have not been investigated. Read More

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December 2018

A mouse model for intellectual disability caused by mutations in the X-linked 2'‑O‑methyltransferase Ftsj1 gene.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Felix-Hausdorff-Strasse 8, 17489 Greifswald, Germany.

Mutations in the X chromosomal tRNA 2'‑O‑methyltransferase FTSJ1 cause intellectual disability (ID). Although the gene is ubiquitously expressed affected individuals present no consistent clinical features beyond ID. In order to study the pathological mechanism involved in the aetiology of FTSJ1 deficiency-related cognitive impairment, we generated and characterized an Ftsj1 deficient mouse line based on the gene trapped stem cell line RRD143. Read More

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December 2018

Multiple integrin ligands provide a highly adhesive and osteoinductive surface that improves selective cell retention technology.

Acta Biomater 2018 Dec 14. Epub 2018 Dec 14.

National & Regional United Engineering Lab of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China; Center of Regenerative and Reconstructive Engineering Technology in Chongqing City, Chongqing, China. Electronic address:

Among various bone tissue engineering strategies, selective cell retention (SCR) technology has been used as a practical clinical method for bone graft manufacturing in real time. The more mesenchymal stem cells (MSCs) are retained, the better the osteoinductive microenvironment provided by the scaffold, which in turn promotes the osteogenesis of the SCR-fabricated bone grafts. Integrin receptors are crucial to cell-matrix adhesion and signal transduction. Read More

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December 2018

Cellular responses to thermoresponsive stiffness memory elastomer nanohybrid scaffolds by 3D-TIPS.

Acta Biomater 2018 Dec 14. Epub 2018 Dec 14.

Centre for Biomaterials in Surgical Reconstruction and Regeneration, Division of Surgery & Interventional Science, University College London, London, NW3 2PF, United Kingdom. Electronic address:

Increasing evidence suggests the contribution of the dynamic mechanical properties of the extracellular matrix (ECM) to regulate tissue remodeling and regeneration. Following our recent study on a family of thermoresponsive 'stiffness memory' elastomeric nanohybrid scaffolds manufactured via an indirect 3D printing guided thermo-induced phase separation process (3D-TIPS), this work reports in vitro and in vivo cellular responses towards these scaffolds with different initial stiffness and hierarchical interconnected porous structure. The viability of mouse embryonic dermal fibroblasts in vitro and the tissue responses during the stiffness softening of the scaffolds subcutaneously implanted in rats for three months were evaluated by immunohistochemistry and histology. Read More

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December 2018

Various strategies to improve efficacy of stem cell transplantation in multiple sclerosis: Focus on mesenchymal stem cells and neuroprotection.

J Neuroimmunol 2018 Dec 1;328:20-34. Epub 2018 Dec 1.

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS) which predominantly affect young adults and undergo heavy socioeconomic burdens. Conventional therapeutic modalities for MS mostly downregulate aggressive immune responses and are almost insufficient for management of progressive course of the disease. Mesenchymal stem cells (MSCs), due to both immunomodulatory and neuroprotective properties have been known as practical cells for treatment of neurodegenerative diseases like MS. Read More

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December 2018

Macrophage functionality and homeostasis in response to oligoethyleneglycol-coated IONPs: impact of a dendritic architecture.

Int J Pharm 2018 Dec 14. Epub 2018 Dec 14.

Université de Strasbourg, CNRS, CAMB UMR 7199, F-67000 Strasbourg, France.

The engineering of iron oxide nanoparticles (IONPs) for biomedical use has received great interest over the past decade. In the present study we investigated the biocompatibility of IONPs grafted with linear (2P) or generation 1 (2PG1) or 2 (2PG2) dendronized oligoethyleneglycol units in THP-1-derived macrophages. To evaluate IONP effects on cell functionality and homeostasis, mitochondrial function (MTT assay), membrane permeability (LDH release), inflammation (IL-8), oxidative stress (reduced glutathione, GSH), NLRP3-inflammasome activation (IL-1β) and nanoparticle cellular uptake (intracellular iron content) were quantified after a 4-h or 24-h cell exposure to increasing IONP concentrations (0-300 µg Fe/mL). Read More

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December 2018
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Dissolvable microneedle patch containing doxorubicin and docetaxel is effective in 4T1 xenografted breast cancer mouse model.

Int J Pharm 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmacy, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, Telangana, India. Electronic address:

Microneedle-devices provide a promising alternative to syringe-injection-based administration of chemotherapeutics. Dissolvable polymeric microneedles provide possibility of carrying greater payload and dual drugs. Here, we report development of polyvinyl pyrrolidone and polyvinyl alcohol composite dissolvable polymeric microneedle system for co-delivery of doxorubicin HCl and docetaxel. Read More

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December 2018
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Methotrexate-loaded biodegradable polymeric micelles for lymphoma therapy.

Int J Pharm 2018 Dec 14. Epub 2018 Dec 14.

Department of Hematology and Research Laboratory of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center, Chengdu, 610041, PR China. Electronic address:

Drug resistance and recurrence are the main clinical challenges in chemotherapy of lymphoma. Methotrexate (MTX), especially high dose MTX (HD MTX), is extensively used to treat some aggressive subtypes of lymphoma, such as Burkitt's lymphoma, in order to overcome drug resistance. But poor solubility of the free drug and severe side effects of HD MTX limit its clinical application. Read More

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December 2018

RGD-modified PEGylated paclitaxel nanocrystals with enhanced stability and tumor-targeting capability.

Int J Pharm 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmaceutics, School of Pharmacy, Fudan University & Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai 201203, China.

Nanocrystals has been constructed for insoluble drugs as a novel type of nanoscale drug delivery systems with high drug loading. How to prepare nanocrystals with good stability and tumor targeting capability is still challenging. This study was to modify paclitaxel nanocrystals with polyethylene glycol (PEG) for stabilization and RGD peptide for tumor targeting. Read More

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December 2018

Single nanomolar doxorubicin exposure triggers compensatory mitochondrial responses in H9c2 cardiomyoblasts.

Food Chem Toxicol 2018 Dec 14. Epub 2018 Dec 14.

CNC - Center for Neuroscience and Cell Biology, University of Coimbra, UC Biotech Building, Biocant Park, 3060-197, Cantanhede, Portugal. Electronic address:

Dose-dependent and cumulative cardiotoxicity associated with doxorubicin (DOX) is the main limitation of anticancer therapy. Pediatric cancer survivors are particularly vulnerable, and no effective prevention measures are available. The aim of the present study was to investigate the persistent effects of nanomolar DOX concentrations and determine whether a pretreatment would induce mitochondrial adaptations in H9c2 cardiomyoblasts. Read More

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December 2018

Alpha-mangostin: Anti-inflammatory and antioxidant effects on established collagen-induced arthritis in DBA/1J mice.

Food Chem Toxicol 2018 Dec 14. Epub 2018 Dec 14.

Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria s/n, Ciudad de México, 04650, Mexico. Electronic address:

Rheumatoid arthritis (RA) is an autoimmune disease that causes physical disability in people worldwide. Despite progress made in RA treatment in the past decade, new drugs with high efficacy but few long-term adverse effects are still needed. This study focused on evaluating the therapeutic potential of α-mangostin on established collagen-induced arthritis (CIA) in DBA/1J mice. Read More

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December 2018

Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University - New Jersey Medical School and Cancer Institute of New Jersey, Newark, NJ 07103, USA. Electronic address:

Disulfide bonds (SS) are post-translational modifications important for the proper folding and stabilization of many cellular proteins with therapeutic uses, including antibodies and other biologics. With budding advances of biologics and biosimilars, there is a mounting need for a robust method for accurate identification of SS. Even though several mass spectrometry methods have emerged for this task, their practical use rests on the broad effectiveness of both sample preparation methods and bioinformatics tools. Read More

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December 2018

Identification of the proteoforms of surface localized Rod A of Aspergillus flavus and determination of the mechanism of proteoform generation.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Proteomics, Aravind Medical Research Foundation, Aravind Eye Care System, Dr. G. Venkataswamy Eye Research Institute, No:1 Anna Nagar, Madurai, TN, India. Electronic address:

Fungal keratitis is a serious, potentially sight-threatening corneal infection that is more prevalent in the tropical parts of the world including India, and A. flavus and Fusarium solani are the predominant etiological agents. The surface of fungal conidia is covered by hydrophobin family proteins, effectively masking the conidial antigens from immune cells. Read More

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December 2018

Dissecting the molecular effects of cigarette smoke on proteasome function.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany. Electronic address:

Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke, one of the main risk factors for COPD, impairs proteasome function in vitro and in vivo. In the present study, we dissected the molecular changes induced by cigarette smoke on the proteasome in lung epithelial cells and mouse lungs. Read More

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December 2018

5-methylcytosine and 5-hydroxymethylcytosine in brains of patients with multiple system atrophy and patients with Parkinson's disease.

J Chem Neuroanat 2018 Dec 14. Epub 2018 Dec 14.

Department of Neurology, University of Bonn, Germany; German Center for Neurodegenerative Diseases (DZNE), University of Bonn, Germany.

Multiple system atrophy (MSA) is a sporadic neurodegenerative disorder of unknown etiology, characterized pathologically by α-synuclein aggregates preferentially found in oligodendroglial cells. DNA methylation has emerged as a mechanism of regulation of α-synuclein expression. Reduced 5-methylcytosine (5-mC) DNA methylation of α-synuclein has been found in the brains of patients with Parkinson's disease (PD). Read More

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December 2018

A papilloma in a large cyst of the breast: A case report.

Int J Surg Case Rep 2018 Nov 16;55:1-3. Epub 2018 Nov 16.

Department of Pediatric, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, South Korea. Electronic address:

Introduction: Intracystic lesions of the breast prove to be benign more often than malignancy, most intracystic lesions are benign papillomas. Generally sizes of cysts that contain papilloma are small. Relatively cysts that contain papillary carcinoma are large. Read More

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November 2018
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Gene cloning, expression, molecular modeling and docking study of the protease SAPRH from Bacillus safensis strain RH12.

Int J Biol Macromol 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour, Km 6, P.O. Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address:

The sapRH gene, which encodes the serine alkaline protease SAPRH, from Bacillus safensis RH12, was isolated and its DNA sequence was determined. The deduced amino-acid sequence showed strong homology with other Bacillus proteases. The highest sequence identity value (97%) was obtained with SAPB from B. Read More

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December 2018

Role of anoctamin 5, a gene associated with gnathodiaphyseal dysplasia, in osteoblast and osteoclast differentiation.

Bone 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Republic of Korea; Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Republic of Korea. Electronic address:

Anoctamin 5 (Ano5) mutations are responsible for gnathodiaphyseal dysplasia, a rare skeletal syndrome. Despite the close linkage of Ano5 to bone remodeling, the molecular mechanisms underlying the role of Ano5 in bone remodeling remain unknown. In this study, we investigated whether Ano5 regulates osteoblast or osteoclast differentiation to maintain normal bone remodeling. Read More

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December 2018

Effective Effectors: How T cells Access and Infiltrate the Central nervous System.

Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.

Duke Brain Tumor Immunotherapy Program, Department of Neurosurgery, Duke University Medical Center, Durham, NC 27710., United States; The Preston Robert Tisch Brain Tumor Center, Duke University Medical Center, Durham, NC 27710., United States; Department of Neurosurgery, Duke University School of Medicine, Durham, NC 27710., United States; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27710, United States; Department of Pathology, Duke University School of Medicine, Durham, NC 27710., United States. Electronic address:

Several Phase II and III clinical trials have demonstrated that immunotherapy can induce objective responses in otherwise refractory malignancies in tumors outside the central nervous system. In large part, effector T cells mediate much of the antitumor efficacy in these trials, and potent antitumor T cells can be generated through vaccination, immune checkpoint blockade, adoptive transfer, and genetic manipulation. However, activated T cells must still traffic to, infiltrate, and persist within tumor in order to mediate tumor lysis. Read More

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December 2018
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KIT as a therapeutic target for non-oncological diseases.

Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.

Aix Marseille Université, INSERM, INRA, C2VN, Marseille, France; Clinique des Bronches, Allergies et Sommeil, Hôpital Nord, AP-, HM, Marseille, France.. Electronic address:

KIT is a receptor tyrosine kinase that after binding to its ligand stem cell factor activates signaling cascades linked to biological processes such as proliferation, differentiation, migration and cell survival. Based on studies performed on SCF and/or KIT mutant animals that presented anemia, sterility, and/or pigmentation disorders, KIT signaling was mainly considered to be involved in the regulation of hematopoiesis, gametogenesis, and melanogenesis. More recently, novel animal models and ameliorated cellular and molecular techniques have led to the discovery of a widen repertoire of tissue compartments and functions that are being modulated by KIT. Read More

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December 2018
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