10,113 results match your criteria Cellular Immunology [Journal]


Cigarette smoke extract acts directly on CD4 T cells to enhance Th1 polarization and reduce memory potential.

Cell Immunol 2018 Jun 18. Epub 2018 Jun 18.

Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA. Electronic address:

Although cigarette smoke is known to alter immune responses, whether and how CD4 T cells are affected is not well-described. We aimed to characterize how exposure to cigarette smoke extract impacts CD4 T cell effector generation in vitro under Th1-polarizing conditions. Our results demonstrate that cigarette smoke directly acts on CD4 T cells to impair effector expansion by decreasing division and increasing apoptosis. Read More

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Murine regulatory T cells induce death of effector T, B, and NK lymphocytes through a contact-independent mechanism involving telomerase suppression and telomere-associated senescence.

Cell Immunol 2018 Jun 19. Epub 2018 Jun 19.

Institute of Biomedical Chemistry, Pogodinskaya st 10/8, 119121 Moscow, Russia.

Regulatory T cells (Tregs) suppress the activity of effector T, B and NK lymphocytes and sustain immunological tolerance, but the proliferative activity of suppressed cells remains unexplored. In the present study, we report that mouse Tregs can induce replicative senescence and the death of responder mouse CD4CD25 T cells, CD8 T cells, B cells and NK cells in vitro and in vivo. Contact-independent in vitro co-cultivation with Tregs up-regulated endonuclease G (EndoG) expression and its translocation to the nucleus in responder cells. Read More

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A novel AXL chimeric antigen receptor endows T cells with anti-tumor effects against triple negative breast cancers.

Cell Immunol 2018 May 14. Epub 2018 May 14.

Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China. Electronic address:

Identifying targets for chimeric antigen receptor-modulated T lymphocyte (CAR-T) therapy against solid tumors is an urgent problem to solve. In this study, we showed for the first time that the receptor tyrosine kinase, AXL, is overexpressed in various tumor cell lines and patient tumor tissues including triple negative breast cancer (TNBC) cell lines and patient samples, making AXL a potent novel target for cancer therapy, specifically for TNBC treatment. We also engineered T cells with a CAR consisting of a novel single-chain variable fragment against AXL and revealed its antigen-specific cytotoxicity and ability to release cytokines in a TNBC cell line and other AXL-positive tumors in vitro. Read More

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A distinct dendritic cell population arises in the thymus of IL-13Rα1-sufficient but not IL-13Rα1-deficient mice.

Cell Immunol 2018 Jun 18. Epub 2018 Jun 18.

Department of Molecular Microbiology & Immunology, University of Missouri, MO, USA; Department of Child Heath, University of Missouri, MO, USA; Department of Neurology, University of Missouri, MO, USA. Electronic address:

IL-13 receptor alpha 1 (IL-13Rα1) associates with IL-4Rα to form a functional IL-4Rα/IL-13Rα1 heteroreceptor (HR) through which both IL-4 and IL-13 signal. Recently, HR expression was associated with the development of M2 type macrophages which function as antigen presenting cells (APCs). Herein, we show that a subset of thymic resident dendritic cells (DCs) expressing high CD11b (CD11b) and intermediate CD11c (CD11c) arise in HR-sufficient but not HR-deficient mice. Read More

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Bone marrow-derived inflammatory and steady state DCs are different in both functions and survival.

Cell Immunol 2018 Jun 7. Epub 2018 Jun 7.

Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China. Electronic address:

Dendritic cells (DCs) contain heterogeneous populations, with classical DCs developed at steady state and monocyte-derived DCs mobilized under inflammatory conditions, although their total numbers in vivo are scares. To obtain enough quantity for immunological study or clinical application, we have previously established that bone marrow-derived DCs in the presence of Flt-3L (FL-DCs) or GM-CSF (GM-DCs) in vitro are equivalent to the steady state DCs and inflammatory DCs in vivo respectively. What difference, however, exists between these two most commonly used culture systems in DC functions and survival, and how does it correlate to the division of works by their corresponding counterparts in vivo remain ill-defined. Read More

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The role of exosomes in allograft immunity.

Cell Immunol 2018 Jun 7. Epub 2018 Jun 7.

Extracellular vesicles are emerging as potent vehicles of intercellular communication. In this review, we focus on a subclass of extracellular vesicles called exosomes. Previously considered an unimportant catch-all, exosomes have recently been recognized for their role in various diseases and their potential for therapeutic use. Read More

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June 2018
1 Read

Activation and propagation of tumor-infiltrating lymphocytes from malignant pleural effusion and ascites with engineered cells for costimulatory enhancement.

Cell Immunol 2018 Apr 17. Epub 2018 Apr 17.

The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China. Electronic address:

Adoptive cell therapy (ACT) of autologous tumor-infiltrating lymphocytes (TILs) has shown an effect on mediating tumor regression in some patients with highly advanced, refractory metastatic malignancy. Here, the in vitro generation of TILs isolated from malignant pleural effusion and ascites was compared with which using engineered cells for costimulatory enhancement (ECCE) and 3 common γ-chain cytokines, interleukin (IL)-2, IL-7, and IL-15, alone or in combination. We showed the robust clinical-scale production of TILs with a less differentiated 'young' phenotype by expansion in the presence of ECCE combined with IL-2/7/15. Read More

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April 2018
1 Read

Phenotypic and functional changes of GM-CSF differentiated human macrophages following exposure to apoptotic neutrophils.

Cell Immunol 2018 Jun 7. Epub 2018 Jun 7.

Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address:

The engulfment of apoptotic cells by monocytes and unprimed macrophages results in M2 polarization. In the current study, we investigated whether apoptotic cells influence the phenotypic and functional characteristics of GM-CSF-differentiated human macrophages (GM-Mφ). Our results demonstrate that GM-Mφ preincubated with apoptotic neutrophils (GM-Mφ) show significantly increased expression of CD206 and FasL and decreased capacity to stimulate allogeneic T-cell proliferation thus adopting M2 features. Read More

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LAIR-1 activation inhibits inflammatory macrophage phenotype in vitro.

Cell Immunol 2018 May 31. Epub 2018 May 31.

Department of Immunology, The Fourth Military Medical University, Xi'an 710032, China. Electronic address:

Macrophages are key cell types of innate immunity and play a central role in inflammation and host defense. Leukocyte-associated Ig-like receptor-1 (LAIR-1) is highly expressed on macrophages and regulates macrophage functions in several conditions. However, whether LAIR-1 is involved in governing macrophage polarization is still not clear. Read More

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Too sweet to resist: Control of immune cell function by O-GlcNAcylation.

Cell Immunol 2018 Jun 2. Epub 2018 Jun 2.

Department of Pathology, School of Medicine, Case Western Reserve University, 6526, Wolstein Research Building, 2103 Cornell Road, Cleveland, OH 44106, USA. Electronic address:

O-linked β-N-acetyl glucosamine modification (O-GlcNAcylation) is a dynamic, reversible posttranslational modification of cytoplasmic and nuclear proteins. O-GlcNAcylation depends on nutrient availability and the hexosamine biosynthetic pathway (HBP), which produces the donor substrate UDP-GlcNAc. O-GlcNAcylation is mediated by a single enzyme, O-GlcNAc transferase (OGT), which adds GlcNAc and another enzyme, O-GlcNAcase (OGA), which removes O-GlcNAc from proteins. Read More

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Evaluation of a Leishmania hypothetical protein administered as DNA vaccine or recombinant protein against Leishmania infantum infection and its immunogenicity in humans.

Cell Immunol 2018 May 29. Epub 2018 May 29.

Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address:

Visceral leishmaniasis (VL) is a fatal disease when acute and untreated. The treatment against this disease is long and presents toxicity and/or high costs. Moreover, parasite resistance has been increasing. Read More

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May 2018
1 Read

MFG-E8 inhibits Aβ-induced microglial production of cathelicidin-related antimicrobial peptide: A suitable target against Alzheimer's disease.

Cell Immunol 2018 May 24. Epub 2018 May 24.

Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, People's Republic of China.

Neuroinflammation plays a pivotal role in the incidence and progression of Alzheimer's disease (AD). Cathelicidin-related antimicrobial peptide (CRAMP) is critically involved in the innate neuronal responses of chronic neuroinflammation in AD and thus plays a key role in the disease. Here, we show that Aβ42 induced microglial production of CRAMP, which was effectively inhibited by milk-fat globule-epidermal growth factor 8 (MFG-E8). Read More

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Engineered CAR T cells targeting mesothelin by piggyBac transposon system for the treatment of pancreatic cancer.

Cell Immunol 2018 Jul 30;329:31-40. Epub 2018 Apr 30.

Xinyuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; Shanghai Cell Therapy Research Institute, Shanghai Engineering Research Center for Cell Therapy, Shanghai 201805, China; Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University of Chinese PLA, Shanghai 201805, China. Electronic address:

Patients with pancreatic cancer have a poor prognosis largely due to the poor efficacy of the available treatment modalities. In this study, we engineered mesothelin-targeting chimeric antigen receptor T cells (mesoCAR T) using the piggyBac transposon based plasmid electroporation technique for specific targeting of pancreatic cancer cells expressing mesothelin. In vitro, mesoCAR T cells exhibited rapid and robust killing effect against ASPC1 cells with high expression levels of mesothelin with high production of IFN-γ; the cytotoxic effect on PANC1 cells with low expressions of mesothelin was relatively attenuated. Read More

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Macrophages in obesity.

Cell Immunol 2018 May 3. Epub 2018 May 3.

Obesity Laboratory, Instituto Gulbenkian de Ciência, Oeiras 2780-156, Portugal; The Howard Hughes Medical Institute (HHMI) International Research Scholars Program, Portugal; Department of Physiology, Anatomy, and Genetics, University of Oxford, OX1 3PT, UK. Electronic address:

Obesity is a worldwide public health concern yet no safe therapies are currently available. The activity of sympathetic neurons is necessary and sufficient for fat mass reduction, via norepinephrine (NE) signaling. Macrophage accumulation in the adipose tissue is thought to play the central role in the onset of obesity, yet their relation to NE has been controversial. Read More

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May 2018
2 Reads

Intravital imaging of cutaneous immune responses.

Cell Immunol 2018 May 25. Epub 2018 May 25.

Singapore Immunology Network (SIgN) and Institute of Medical Biology (IMB), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore; Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. Electronic address:

Various immune cells are present in the skin and modulate the cutaneous immune response. In order to capture such dynamic phenomena, intravital imaging is an important technique and there is a possibility to provide substantial information that is not available using conventional histological analysis. Multiphoton microscope enable direct, three-dimensional, minimally invasive imaging of biological samples with high spatiotemporal resolution, and now become the main method for intravital imaging studies. Read More

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May 2018
1 Read

Monocytic myeloid-derived suppressor cells generated from rhesus macaque bone marrow enrich for regulatory T cells.

Cell Immunol 2018 Jul 27;329:50-55. Epub 2018 Apr 27.

Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. Electronic address:

Putative monocytic myeloid-derived suppressor cells (mMDSC; lineageHLA-DR) were generated in 7-day cultures from normal rhesus macaque bone marrow (BM) cells in GM-CSF and IL-6. Three subsets were identified based on their differential expression of CD14, CD33, CD34 and CD11b. Following flow sorting, assessment of the capacity of these subsets to suppress anti-CD3/CD28-stimulated CD4 and CD8 T cell proliferation revealed that the most potent population was CD14CD33CD34CD11b. Read More

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Aire is not essential for regulating neuroinflammatory disease in mice transgenic for human autoimmune-diseases associated MHC class II genes HLA-DR2b and HLA-DR4.

Cell Immunol 2018 May 14. Epub 2018 May 14.

Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, United States. Electronic address:

The human autoimmune disease-associated HLA alleles HLA-DR2b (DRB1*1501) and HLA-DR4 (DRB1*0401) are strongly linked to increased susceptibility for multiple sclerosis (MS) and rheumatoid arthritis (RA), respectively. The underlying mechanisms are not fully understood, but these MHC alleles may shape the repertoire of pathogenic T cells via central tolerance. The transcription factor autoimmune regulator (AIRE) promotes central T cell tolerance via ectopic expression of tissue-specific antigens (TSAs). Read More

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The non-psychoactive phytocannabinoid cannabidiol (CBD) attenuates pro-inflammatory mediators, T cell infiltration, and thermal sensitivity following spinal cord injury in mice.

Cell Immunol 2018 Jul 8;329:1-9. Epub 2018 Mar 8.

Center for Substance Abuse Research, Department of Pharmacology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, United States. Electronic address:

We evaluated the effects of the non-psychoactive cannabinoid cannabidiol (CBD) on the inflammatory response and recovery of function following spinal cord injury (SCI). Female C57Bl/6 mice were exposed to spinal cord contusion injury (T9-10) and received vehicle or CBD (1.5 mg/kg IP) injections for 10 weeks following injury. Read More

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Immunosurveillance of cancer and the heat shock protein-CD91 pathway.

Authors:
Robert J Binder

Cell Immunol 2018 May 16. Epub 2018 May 16.

Department of Immunology, University of Pittsburgh, Pittsburgh, PA 15261, United States. Electronic address:

The intracellular functions of heat shock proteins (HSPs) as chaperones of macromolecules are well known. Current observations point to a role of these chaperones in initiating and modulating immune responses to tumors via receptor(s) on dendritic cells. In this article we provide an insight into, and a basis for, the importance of these HSP-mediated immune responses in rejecting nascent and emerging tumors. Read More

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Origin and specialization of splenic macrophages.

Cell Immunol 2018 May 15. Epub 2018 May 15.

Instituto Investigaciones Biomédicas "Alberto Sols", Centro Mixto Consejo Superior de Investigaciones Cientificas y Universidad Autonoma de Madrid (IIBM CSIC-UAM), IIBM Madrid, Spain; Unidad De Biomedicina (Unidad Asociada al CSIC), IIBM- Universidad Las Palmas de Gran Canaria, ULPGC, Grupo de Investigación en medio ambiente y Salud (GIMAS), Instituto Universitario de Investigaciones Biomedicas y Sanitarias (IUIBS, ULPGC), Spain.

Macrophage heterogeneity in the spleen has been long documented, with four subsets populating the different splenic compartments. The diverse environments on the splenic compartments determine their varied phenotype and functions. In the white pulp, highly phagocytic macrophages contribute to the generation of the immune response. Read More

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Immune complexes suppressed autophagy in glomerular endothelial cells.

Cell Immunol 2018 Jun 21;328:1-8. Epub 2018 Feb 21.

Department of Health Technology and Informatics, Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China. Electronic address:

Lupus nephritis is an immune-complexes mediated glomerulonephritis. Vascular lesions and endothelial cell injuries are common in lupus nephritis and important for renal damage. However, the precise mechanisms by which immune complexes lead to endothelial cell injuries are still unclear. Read More

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June 2018
1 Read

Early Env-specific CTLs effectively suppress viral replication in SHIV controller macaques.

Cell Immunol 2018 May 5. Epub 2018 May 5.

Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Haidian District, Beijing 100191, China; State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Changping District, Beijing 102206, China. Electronic address:

Early immunological events in acute HIV infection are thought to fundamentally influence long-term disease outcomes. Though the contribution of Gag-specific CD8 T cell responses to early viral control is well established, little is known about the role of Env-specific CD8 T cell responses in controlling viral replication during acute infection. In a macaque simian-human immunodeficiency virus (SHIV) model, some macaques who were able to control SHIV replication after ART interruption showed expansion of Env-specific CD8 T cell responses during acute infection, compared to macaques who progressed to viral rebound. Read More

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Emerging glycobiology tools: A renaissance in accessibility.

Cell Immunol 2018 Apr 25. Epub 2018 Apr 25.

Case Western Reserve University School of Medicine, Department of Pathology, 10900 Euclid Avenue, Cleveland, OH 44106, USA. Electronic address:

The glycobiology of the immune response is a topic that has garnered increased attention due to a number of key discoveries surrounding IgG function, the specificity of some broadly neutralizing anti-HIV antibodies, cancer immunoregulation by galectin molecules and others. This review is the opening article in a Special Edition of Cellular Immunology focused on glycoimmunology, and has the goal of setting the context for these articles by providing a mini-review of how glycans impact immunity. We also focus on some of the technological and methodological advances in the field of glycobiology that are being deployed to lower the barrier of entry into the glycosciences, and to more fully interrogate the glycome and its function. Read More

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Biochemical characterization and immunogenicity of Neureight, a recombinant full-length factor VIII produced by fed-batch process in disposable bioreactors.

Cell Immunol 2018 May 5. Epub 2018 May 5.

INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris F-75006 France; Université Pierre et Marie Curie-Paris6, UMR S 1138, Centre de Recherche des Cordeliers, Paris F-75006 France; Université Paris Descartes, UMR S 1138, Centre de Recherche des Cordeliers, Paris F-75006 France. Electronic address:

Hemophilia A is a X-linked recessive bleeding disorder consecutive to the lack of circulating pro-coagulant factor VIII (FVIII). The most efficient strategy to treat or prevent bleeding in patients with hemophilia A relies on replacement therapy using exogenous FVIII. Commercially available recombinant FVIII are produced using an expensive perfusion technology in stainless steel fermenters. Read More

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May 2018
3 Reads

The multifaceted role of the renal mononuclear phagocyte system.

Cell Immunol 2018 Apr 22. Epub 2018 Apr 22.

Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany. Electronic address:

The kidney contains a large and complex network of mononuclear phagocytes, which includes dendritic cells (DCs) and macrophages (MØs). The distinction between these cell types is traditionally based on the expression of molecular markers and morphology. However, several classification systems are used in parallel to identify DCs and MØs, leading to considerable uncertainty about their identity and functional roles. Read More

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NFKB1 mediates Th1/Th17 activation in the pathogenesis of psoriasis.

Cell Immunol 2018 May 2. Epub 2018 May 2.

Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, No. 81 Meishan Road, Hefei, Anhui 230032, China; Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui 230032, China. Electronic address:

This study was aimed to investigate whether NFKB1 participates in the pathogenesis of psoriasis by mediating Th1/Th17 cells. In this study, expression of NFKB1 was assessed in skin tissues from psoriasis patients and the healthy controls through Western blot and Immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the serum levels of IFN-γ, IL-17 (IL-17A) and IL-17RA. Read More

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Deletion of the C6orf120 gene with unknown function ameliorates autoimmune hepatitis induced by concanavalin A.

Cell Immunol 2018 May 2. Epub 2018 May 2.

Department of Center of Integrated Traditional Chinese and Western Medicine, Peking University Ditan Teaching Hospital, Beijing 100015, China; Department of Center of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. Electronic address:

The present study was conducted to characterize the C6orf120 gene, by using C6orf120 gene-deleted rats (C6orf120), to determine its role in the development and severity of autoimmune hepatitis induced by concanavalin A (Con A), as well as the underlying mechanisms. We found that following Con A injection, C6orf120 rats were less susceptible to developing autoimmune hepatitis with low levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) post challenge. Additionally, C6orf120 deficiency increased the frequency of cluster of differentiation (CD)4 CD25 Forkhead box P3 regulatory T cells (Tregs) among intrahepatic lymphocytes, splenocytes, peripheral blood mononuclear cells, and CD4 T in vitro. Read More

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May 2018
1.92 Impact Factor

Modulation of immune responses in lentiviral vector-mediated gene transfer.

Cell Immunol 2018 Apr 27. Epub 2018 Apr 27.

San Raffaele Telethon Institute for Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan, Italy; Vita Salute San Raffaele University, Milan, Italy. Electronic address:

Lentiviral vectors (LV) are widely used vehicles for gene transfer and therapy in pre-clinical animal models and clinical trials with promising safety and efficacy results. However, host immune responses against vector- and/or transgene-derived antigens remain a major obstacle to the success and broad applicability of gene therapy. Here we review the innate and adaptive immunological barriers to successful gene therapy, both in the context of ex vivo and in vivo LV gene therapy, mostly concerning systemic LV delivery and discuss possible means to overcome them, including vector design and production and immune modulatory strategies. Read More

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Class I PI3K inhibitor ZSTK474 attenuates experimental autoimmune neuritis by decreasing the frequency of Th1/Th17 cells and reducing the production of proinflammatory cytokines.

Cell Immunol 2018 Jul 26;329:41-49. Epub 2018 Apr 26.

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address:

The Class I phosphatidylinositol 3-kinase inhibitor, 2-(2-difluoromethy lbenzimidazol-1-yl)-4,6-dimorpholino-1,3,5-triazine (ZSTK474), has anti-inflammatory and immunoregulatory properties. However, whether it can be used to treat Guillain-Barré syndrome (GBS)-a neuroinflammatory disorder-is unknown. We induced experimental autoimmune neuritis (EAN) in Lewis rats, an established model of GBS. Read More

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Analysis of epitope-based vaccine candidates against the E antigen of the hepatitis B virus based on the B genotype sequence: An in silico and in vitro approach.

Cell Immunol 2018 Jul 30;329:56-65. Epub 2018 Apr 30.

Department of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address:

Chronic hepatitis B virus infection is a worldwide health problem with no current effective strategy to achieve a cure. The Hepatitis B virus (HBV) E antigen (HBeAg) has a negative effect on the immune system and a therapeutic vaccine is a promising strategy in order to treat chronic virus infection. In this study, we analyzed and identified the MHC-I, MHC-II and B cell epitopes of the HBeAg based on a B genotype sequence of HBV using a bioinformatic approach and in vitro experiments. Read More

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New concepts in macrophage ontogeny in the adult neural retina.

Authors:
Daniel R Saban

Cell Immunol 2018 Apr 22. Epub 2018 Apr 22.

Duke University School of Medicine, Department of Ophthalmology, Durham, NC, USA; Duke University School of Medicine, Department of Immunology, Durham, NC, USA. Electronic address:

The number of neurons dedicated to vision itself is thought to be greater than the sum of the four other senses combined. Yet, little attention has been payed to the retina as compared to elsewhere in the central nervous system with respect to microglia, the macrophages of the neural parenchyma. Indeed, major advancements in the understanding of microglial ontogeny and maintenance in brain and spinal cord are now widely appreciated, whereas less notice has been given to the neural retina in this regard. Read More

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IgG and leukocytes: Targets of immunomodulatory α2,6 sialic acids.

Authors:
Mark B Jones

Cell Immunol 2018 Mar 31. Epub 2018 Mar 31.

Case Western Reserve University, School of Medicine, Department of Pathology, Cleveland, OH 44106, United States. Electronic address:

ST6Gal1 is a critical sialyltransferase enzyme that controls the addition of α2,6-linked sialic acids to the termini of glycans. Attachment of sialic acids to glycoproteins as a posttranslational modification influences cellular responses, and is a well-known modifier of immune cell behavior. ST6Gal1 activity impacts processes such as: effector functions of immunoglobulin G via Fc sialylation, hematopoietic capacity by hematopoietic stem and progenitor cell surface sialylation, and lymphocyte activation thresholds though CD22 engagement and inhibition of galectins. Read More

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Intranasal delivery of influenza antigen by nanoparticles, but not NKT-cell adjuvant differentially induces the expression of B-cell activation factors in mice and swine.

Cell Immunol 2018 Jul 11;329:27-30. Epub 2018 Apr 11.

Food Animal Health Research Program, Ohio Agricultural Research and Development Center (OARDC), 1680 Madison Avenue, Wooster, OH 44691, USA; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA. Electronic address:

Intranasal vaccination of pigs with poly lactic-co-glycolic acid and polyanhydride nanoparticles delivered inactivated influenza virus provides cross-reactive T-cell response, but not antibody response, resulting in incomplete protection and no reduction in nasal virus shedding. Expression of BAFF and Th2 transcription factor GATA-3 were downregulated in lungs of pigs vaccinated with influenza nanovaccine, but in mice it upregulated the expression of BAFF and cytokine TGFβ in cervical lymph nodes. However, the intranasal iNKT cell adjuvant, α-Galctosylceramide upregulates the expression of BAFF in pig lungs. Read More

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July 2018
1 Read

The role of hepatic macrophages in liver metastasis.

Cell Immunol 2018 Apr 6. Epub 2018 Apr 6.

Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, Brussels, Belgium; Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium. Electronic address:

The liver is a major target organ for metastasis of both gastrointestinal and extra-gastrointestinal cancers. Due to its frequently inoperable nature, liver metastasis represents a leading cause of cancer-associated death worldwide. In the past years, the pivotal role of the immune system in this process is being increasingly recognised. Read More

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April 2018
1 Read

Silk fibroin preserves beta cell function under inflammatory stress while stimulating islet cell surface GLUT2 expression.

Cell Immunol 2018 Jul 9;329:10-16. Epub 2018 Apr 9.

Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. Electronic address:

Silk fibroin is a novel biomaterial for enhancing transplanted islet cell function and survival. This study investigated whether silk fibroin may have unique properties that improve islet function in the face of inflammatory-mediated stress during transplantation. Murine islet function was tested in vitro with either silk fibroin or alginate and challenged with inflammatory cytokines. Read More

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July 2018
1 Read

Protective efficacy of a plasmid DNA vaccine against transgene-specific tumors by Th1 cellular immune responses after intradermal injection.

Cell Immunol 2018 Jul 9;329:17-26. Epub 2018 Apr 9.

Department of Plastic and Reconstructive Surgery, Seoul National University Boramae Medical Center, Seoul, Republic of Korea. Electronic address:

With DNA vaccines, it is important to monitor the movement of transfectants and to overcome immune deviations. We used a pCMV-LacZ plasmid (expressing β-galactosidase) and a pcDNA-hNIS plasmid (expressing the human sodium/iodide symporter [hNIS] gene) as non-secreted visual-imaging markers. Transfectants carrying the hNIS or LacZ gene migrated to peripheral lymphoid tissues. Read More

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July 2018
3 Reads

LPS enhances platelets aggregation via TLR4, which is related to mitochondria damage caused by intracellular ROS, but not extracellular ROS.

Cell Immunol 2018 Jun 7;328:86-92. Epub 2018 Apr 7.

Department of Hematology, The Third Xiangya Hospital, Central South University, No. 138 Tongzi Road, Changsha, Hunan, PR China. Electronic address:

Platelet is an important cell contributing to hemostasis and immunity. Bacterial lipopolysaccharide (LPS), mainly functioning by stimulating toll-like receptor 4 (TLR4), mediates platelet activation and sepsis. However, the inter-relationship between these players in sepsis remains unknown. Read More

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June 2018
6 Reads

Heart macrophages and dendritic cells in sickness and in health: A tale of a complicated marriage.

Cell Immunol 2018 Apr 5. Epub 2018 Apr 5.

Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine, Ghent University, Ghent, Belgium; Department of Pulmonary Medicine, ErasmusMC, Rotterdam, The Netherlands.

Heart disease is the major cause of death and it is broadly recognized that the immune system plays a central role in healthy and injured heart. Here, we focus on the contribution of various subsets of mononuclear phagocytes in the cardiac system. Macrophages and dendritic cells reside in the healthy myocardium to fulfill homeostatic functions and rapidly increase in numbers in diseases like myocardial ischemia and myocarditis to contribute to disease or resolve it. Read More

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April 2018
1 Read

Testicular macrophages: Guardians of fertility.

Cell Immunol 2018 Apr 3. Epub 2018 Apr 3.

Centre d'Immunologie de Marseille Luminy, Aix-Marseille University, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Marseille, France; Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtzgemeinschaft (MDC), Robert-Rössle-Strasse 10, 13125 Berlin, Germany. Electronic address:

Macrophages are innate immune cells present in essentially every organ of the body with dedicated tissue specific functions. We will present in this review the unique properties and functions of macrophage populations residing in the testis, an immune-privileged organ. Testicular macrophages (tMΦ) could be seen as guardians of fertility due to their immunosuppressive functions protecting spermatogenesis from auto immune-attack. Read More

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April 2018
1 Read

4-1BB expression on MAIT cells is associated with enhanced IFN-γ production and depends on IL-2.

Cell Immunol 2018 Jun 31;328:58-69. Epub 2018 Mar 31.

Division of Research, Institute of Tuberculosis, 309(th) Hospital, Beijing, China. Electronic address:

The role of MAIT cells in immunity against Mycobacterium tuberculosis infection in humans is still largely unexplored. In this study, we investigated the functional role of 4-1BB on MAIT cells. We found that 4-1BB was highly up-regulated on MAIT cells from tuberculous pleural effusions following Mtb antigen stimulation and its level of expression correlated with IFN-γ and IL-17 production. Read More

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June 2018
2 Reads

High fiber dietary and sodium butyrate attenuate experimental autoimmune hepatitis through regulation of immune regulatory cells and intestinal barrier.

Cell Immunol 2018 Jun 22;328:24-32. Epub 2018 Mar 22.

Department of Infectious Diseases, The First Affiliated Hospital of Wenzhou Medical University, Hepatology Institute of Wenzhou Medical University, Wenzhou Key Laboratory of Hepatology, Wenzhou 325000, Zhejiang, China. Electronic address:

Autoimmune hepatitis (AIH) is chronic autoimmune liver disease accompanied with the imbalance of Treg/Th17 and increased intestinal permeability. We investigated the effects of a high fiber diet and sodium butyrate on the Treg/Th17 and intestinal barrier function in an experimental autoimmune hepatitis. Intraperitoneal injection of hepatic antigen (S100) was used to induce experimental autoimmune hepatitis mice model and mice were divided into normal control, S100 model control, S100 plus high fiber diet and S100 plus sodium butyrate. Read More

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June 2018
5 Reads

Immune active cells with 4-1BB signal enhancement inhibit hepatitis B virus replication in noncytolytic manner.

Cell Immunol 2018 Jun 2;328:79-85. Epub 2018 Apr 2.

Liver Disease Department, The Second Hospital of Nanjing, Affiliated to Medical School of Southeast University, Zhong Fu Road, Gulou District, Nanjing, Jiangsu 210003, PR China. Electronic address:

Immune active cells (IACs) have been shown to be an alternative immunotherapy for CHB patients. However, there is a practical problem of different expansion rate and function of HBV inhibition as individual variability exists. Our previous studies have confirmed that the proliferation and cytolysis of IACs were significantly up-regulated by engineered cells for costimulatory enhancement (ECCE) delivering a 4-1BBζ activating signal. Read More

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June 2018
1 Read

Mycobacterium tuberculosis protein Rv2220 induces maturation and activation of dendritic cells.

Cell Immunol 2018 Jun 31;328:70-78. Epub 2018 Mar 31.

Department of Microbiology, and Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, South Korea. Electronic address:

Tuberculosis remains a serious health problem worldwide. Characterization of the dendritic cell (DC)-activating mycobacterial proteins has driven the development of effective TB vaccine candidates besides improving the understanding of immune responses. Some studies have emphasized the essential role of protein Rv2220 from M. Read More

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June 2018
5 Reads

Galectins: Multitask signaling molecules linking fibroblast, endothelial and immune cell programs in the tumor microenvironment.

Cell Immunol 2018 Mar 21. Epub 2018 Mar 21.

Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), C1428 Ciudad de Buenos Aires, Argentina; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires, C1428 Ciudad de Buenos Aires, Buenos Aires, Argentina. Electronic address:

Tumor cells corrupt surrounding normal cells instructing them to support proliferative, pro-angiogenic and immunosuppressive networks that favor tumorigenesis and metastasis. This dynamic cross-talk is sustained by a range of intracellular signals and extracellular mediators produced by both tumoral and non-tumoral cells. Galectins -whether secreted or intracellularly expressed- play central roles in the tumorigenic process by delivering regulatory signals that contribute to reprogram fibroblasts, endothelial and immune cell programs. Read More

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March 2018
3 Reads

Paramunity-inducing Factors (PINDs) in dendritic cell (DC) cultures lead to impaired antileukemic functionality of DC-stimulated T-cells.

Cell Immunol 2018 Jun 16;328:33-48. Epub 2018 Mar 16.

Dept for Hematopoetic Transplantations, MED3, University of Munich, Germany; Helmholtz Center Munich, CCG-HCT, Munich, Germany. Electronic address:

Introduction: Paramunity-inducing-Factors (PINDs) consist of attenuated/inactivated viruses of various poxvirus-genera, used in veterinary medicine as non-antigen-specific, non-immunising stimulators of the innate immune system against infectious and malignant diseases. Their danger-signaling-interactions were tested for their capacity to improve leukemic antigen-presentation on DC generated from AML-patients' blasts ('DC') and DC-stimulation/activation of antileukemic T-cells.

Methods: We analyzed, whether the addition of PINDs during DC cultures (15 healthy, 22 leukemic donors) and mixed lymphocyte culture (MLC, n = 15) with autologous (n = 6), allogeneic (n = 2) or T-cells after stem cell transplantation (SCT; n = 7) would alter the quality and quantity of DC, the composition of T-cell-subsets, and/or their antileukemic functionality (AF) as studied by FACS and functional Fluorolysis-cytotoxicity-assays. Read More

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June 2018
5 Reads

Glycosylation in cancer: Selected roles in tumour progression, immune modulation and metastasis.

Cell Immunol 2018 Mar 20. Epub 2018 Mar 20.

I3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal; Institute of Molecular Pathology and Immunology, University of Porto, 4200-135 Porto, Portugal; Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar, University of Porto, 4050-313 Porto, Portugal. Electronic address:

Tumour metastasis is the main cause of cancer related deaths. Metastasis is an intricate multi-step process that requires the acquisition of several cancer cell features, including the modulation of tumour cell migration, adhesion, invasion, and immune evasion. Changes in the cellular glycosylation are associated with malignant transformation of cancer cells, tumour progression and ultimately, metastasis formation. Read More

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March 2018
7 Reads

Liver induced transgene tolerance with AAV vectors.

Cell Immunol 2017 Dec 5. Epub 2017 Dec 5.

Department of Pediatrics, Div. Cell and Molecular Therapy, University of Florida, United States; Department of Neuroscience, University of Florida, United States. Electronic address:

Immune tolerance is a vital component of immunity, as persistent activation of immune cells causes significant tissue damage and loss of tolerance leads to autoimmunity. Likewise, unwanted immune responses can occur in inherited disorders, such as hemophilia and Pompe disease, in which patients lack any expression of protein, during treatment with enzyme replacement therapy, or gene therapy. While the liver has long been known as being tolerogenic, it was only recently appreciated in the last decade that liver directed adeno-associated virus (AAV) gene therapy can induce systemic tolerance to a transgene. Read More

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December 2017
2 Reads

Alpha 1-antitrypsin inhibits microglia activation and facilitates the survival of iPSC grafts in hypertension mouse model.

Cell Immunol 2018 Jun 16;328:49-57. Epub 2018 Mar 16.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yet-Sen University, Guangzhou 510060, China. Electronic address:

This study was conducted to investigate the use of Alpha 1-antitrypsin (AAT) to inhibit microglia activation in chronic hypertension model and provide a permissive environment for stem cell transplantation. Chronic ocular hypertension of C57BL/6 mice using magnetic microbead injection was induced 3 weeks prior to iPSCs transplantation. The ocular hypertension model was assessed histologically and intraocular pressure was measured. Read More

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June 2018
7 Reads

Prevalence and long-term monitoring of humoral immunity against adeno-associated virus in Duchenne Muscular Dystrophy patients.

Cell Immunol 2018 Mar 16. Epub 2018 Mar 16.

Genethon and INSERM U951, Evry 91000 France. Electronic address:

Adeno-associated virus (AAV) vectors are promising candidates for gene therapy and have been explored as gene delivery vehicles in the treatment of Duchenne Muscular Dystrophy (DMD). Recent studies showed compelling evidence of therapeutic efficacy in large animal models following the intravenous delivery of AAV vectors expressing truncated forms of dystrophin. However, to translate these results to humans, careful assessment of the prevalence of anti-AAV neutralizing antibodies (NAbs) is needed, as presence of preexisting NABs to AAV in serum have been associated with a drastic diminution of vector transduction. Read More

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March 2018
2 Reads

Myeloid-derived suppressor cells accumulate among myeloid cells contributing to tumor growth in matrix metalloproteinase 12 knockout mice.

Cell Immunol 2018 May 13;327:1-12. Epub 2017 Dec 13.

Vascular Biology Research Institute, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address:

Myeloid-derived suppressor cells (MDSCs) are found frequently in patients and mice bearing tumors, which derived from immature myeloid cells. In healthy individuals, immature myeloid cells formed in the bone marrow differentiating to dendritic cells, macrophages and neutrophils. However, it is unclear whether some gene deficiency will lead to MDSCs accumulation in mice without bearing tumor. Read More

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May 2018
1 Read