372 results match your criteria Cellular And Molecular Bioengineering[Journal]


A Multiscale Model to Predict Neuronal Cell Deformation with Varying Extracellular Matrix Stiffness and Topography.

Cell Mol Bioeng 2020 Jun 4;13(3):229-245. Epub 2020 May 4.

Multiscale Materials Modeling Lab, Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR USA.

Introduction: Neuronal cells are sensitive to mechanical properties of extracellular matrix (ECM) such as stiffness and topography. Cells contract and exert a force on ECM to detect the microenvironment, which activates the signaling pathway to influence the cell functions such as differentiation, migration, and proliferation. There are numerous transmembrane proteins that transmit signals; however, integrin and neural cellular adhesion molecules (NCAM) play an important role in sensing the ECM mechanical properties. Read More

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http://dx.doi.org/10.1007/s12195-020-00615-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225237PMC

Machine Learning Classification of Articular Cartilage Integrity Using Near Infrared Spectroscopy.

Cell Mol Bioeng 2020 Jun 9;13(3):219-228. Epub 2020 Mar 9.

Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.

Introduction: Assessment of cartilage integrity during arthroscopy is limited by the subjective visual nature of the technique. To address this shortcoming in diagnostic evaluation of articular cartilage, near infrared spectroscopy (NIRS) has been proposed. In this study, we evaluated the capacity of NIRS, combined with machine learning techniques, to classify cartilage integrity. Read More

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http://dx.doi.org/10.1007/s12195-020-00612-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225230PMC

Effect of Sintering Temperature of Bioactive Glass Nanoceramics on the Hemolytic Activity and Oxidative Stress Biomarkers in Erythrocytes.

Cell Mol Bioeng 2020 Jun 3;13(3):201-218. Epub 2020 Apr 3.

Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloníki, Greece.

Introduction: The nature of the surface is critical in determining the biological activity of silica powders. A novel correlation between toxicity and surface properties of bioactive glass ceramics (BGCs) synthesized the sol-gel method was attempted in this study.

Methods: The behavior of BGCs after their attachment to the surface of red blood cells (RBCs) was evaluated and their toxic effects were determined based on hemolysis, membrane injury anti-phosphotyrosine immunoblot of Band 3, lipid peroxidation, potential to generate reactive oxygen species, and antioxidant enzyme production. Read More

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http://dx.doi.org/10.1007/s12195-020-00614-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225217PMC

Targeting Dysfunctional Vascular Endothelial Cells Using Immunoliposomes Under Flow Conditions.

Cell Mol Bioeng 2020 Jun 4;13(3):189-199. Epub 2020 May 4.

Department of Chemical and Biomolecular Engineering, Ohio University, 161 Stocker Center, Athens, OH 45701 USA.

Introduction: Atherosclerosis (ATH), the build up of fat in the arteries, is a principal cause of heart attack and stroke. Drug instability and lack of target specificity are major drawbacks of current clinical therapeutics. These undesirable effects can be eliminated by site-specific drug delivery. Read More

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http://dx.doi.org/10.1007/s12195-020-00616-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225235PMC

Evaluation of the Antihemostatic and Antithrombotic Effects of Lowering Coagulation Factor VII Levels in a Non-human Primate.

Cell Mol Bioeng 2020 Jun 23;13(3):179-187. Epub 2020 Mar 23.

Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR USA.

Introduction: Tissue factor (TF) and factor (F) VII, components of the extrinsic pathway of blood coagulation, are essential for hemostatic plug formation in response to injury; less clear are their roles in propagating thrombosis, as observational data in humans with congenital FVII deficiency suggests persistent thrombotic and bleeding risk even at significantly decreased FVII levels. We aimed to define the contribution of FVII to thrombus formation and hemostasis using a non-human primate model.

Methods: We treated baboons with a FVII antisense oligonucleotide (ASO) and measured platelet and fibrin deposition inside and distal to collagen- or TF-coated vascular grafts. Read More

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http://dx.doi.org/10.1007/s12195-020-00613-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225220PMC

A Stable Pep2-proapoptotic Peptide Inducing Apoptosis of Acute Myeloid Leukemia Cells by Down-Regulating EZH2.

Cell Mol Bioeng 2020 Apr 4;13(2):165-177. Epub 2019 Dec 4.

Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, China.

Introduction: Proapoptotic peptide, (KLAKLAK), exhibits strong anti-tumor effect with the help of cell-penetrating peptides such as Pep2, targeting TLR2 with high expression in acute myeloid leukemia (AML). However, the applications are limited due to the peptide's instability and high cost of synthesis. Recombinant PP7 bacteriophage-like particles (VLPs) can protect the peptides from degradation by proteases, based on their ability to display foreign peptides. Read More

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http://dx.doi.org/10.1007/s12195-019-00605-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048892PMC

Transplantation of IL-10-Overexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorates Diabetic-Induced Impaired Fracture Healing in Mice.

Cell Mol Bioeng 2020 Apr 23;13(2):155-163. Epub 2019 Dec 23.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, 200233 China.

Background: Diabetes mellitus is characterized by hyperglycemia which displays insufficiency or resistance to insulin. One of the complications of diabetes is the increased risk of fracture and the impairment of bone repair and regulation. There have been evidences from previous studies that mesenchymal stem cells (MSCs) from bone marrow promote cartilage and callous formation. Read More

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http://dx.doi.org/10.1007/s12195-019-00608-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048895PMC

Localization of Rolling and Firm-Adhesive Interactions Between Circulating Tumor Cells and the Microvasculature Wall.

Cell Mol Bioeng 2020 Apr 24;13(2):141-154. Epub 2020 Jan 24.

Department of Biomedical Engineering, Duke University, Durham, NC USA.

Introduction: The adhesion of tumor cells to vessel wall is a critical stage in cancer metastasis. Firm adhesion of cancer cells is usually followed by their extravasation through the endothelium. Despite previous studies identifying the influential parameters in the adhesive behavior of the cancer cell to a planer substrate, less is known about the interactions between the cancer cell and microvasculature wall and whether these interactions exhibit organ specificity. Read More

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http://dx.doi.org/10.1007/s12195-020-00610-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048902PMC

Microvascular Mimetics for the Study of Leukocyte-Endothelial Interactions.

Cell Mol Bioeng 2020 Apr 31;13(2):125-139. Epub 2020 Jan 31.

Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627 USA.

Introduction: The pathophysiological increase in microvascular permeability plays a well-known role in the onset and progression of diseases like sepsis and atherosclerosis. However, how interactions between neutrophils and the endothelium alter vessel permeability is often debated.

Methods: In this study, we introduce a microfluidic, silicon-membrane enabled vascular mimetic (μSiM-MVM) for investigating the role of neutrophils in inflammation-associated microvascular permeability. Read More

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http://dx.doi.org/10.1007/s12195-020-00611-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048879PMC

Sticking to the Problem: Engineering Adhesion in Molecular Endoscopic Imaging.

Cell Mol Bioeng 2020 Apr 21;13(2):113-124. Epub 2020 Jan 21.

Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH 45701 USA.

Cancers of the digestive tract cause nearly one quarter of the cancer deaths worldwide, and nearly half of these are due to cancers of the esophagus and colon. Early detection of cancer significantly increases the rate of survival, and thus it is critical that cancer within these organs is detected early. In this regard, endoscopy is routinely used to screen for transforming/cancerous (i. Read More

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http://dx.doi.org/10.1007/s12195-020-00609-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048886PMC

Tissue Targeting and Ultrasound-Targeted Microbubble Destruction Delivery of Plasmid DNA and Transfection .

Cell Mol Bioeng 2020 Feb 4;13(1):99-112. Epub 2019 Sep 4.

Key Laboratory of Biomedical Materials and Implant Devices, Research Institute of Tsinghua University in Shenzhen, Nanshan Hi-new Technology and Industry Park, Shenzhen, 518057 Guangzhou People's Republic of China.

Introduction: Ultrasound-targeted microbubble destruction (UTMD) has been shown a promising approach for target-specific gene delivery and treatment of many diseases in the past decade. To improve the therapeutic potential of UTMD, the gene carrier of microbubbles should possess adequate DNA condensation capability and (or) specific cell or tissue selectivity. The tissue-targeted and ultrasound-targeted cationic microbubbles were developed to meet gene therapy. Read More

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http://dx.doi.org/10.1007/s12195-019-00597-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981334PMC
February 2020

Producing Collagen Micro-stripes with Aligned Fibers for Cell Migration Assays.

Cell Mol Bioeng 2020 Feb 25;13(1):87-98. Epub 2019 Sep 25.

Laboratory for Complex Fluids and Interfaces, Mechanobiology and Soft Matter Group, Research Institute for Biosciences, University of Mons, Place du Parc, 20, 7000 Mons, Belgium.

Introduction: The orientation of collagen fibers in native tissues plays an important role in cell signaling and mediates the progression of tumor cells in breast cancer by a contact guidance mechanism. Understanding how migration of epithelial cells is directed by the alignment of collagen fibers requires assays with standardized orientations of collagen fibers.

Methods: To address this issue, we produced micro-stripes with aligned collagen fibers using an easy-to-use and versatile approach based on the aspiration of a collagen solution within a microchannel. Read More

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http://dx.doi.org/10.1007/s12195-019-00600-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981333PMC
February 2020

Synergistic Adhesiveness of Fibronectin with PHSRN Peptide in Gelatin Mixture Promotes the Therapeutic Potential of Human ES-Derived MSCs.

Cell Mol Bioeng 2020 Feb 2;13(1):73-86. Epub 2019 Dec 2.

Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 03722 Republic of Korea.

Introduction: Mesenchymal stem cells (MSCs) are promising candidates for cell therapy owing to their therapeutic effect in various diseases. In general, MSCs grow efficiently in serum-containing culture media, indicating an essential role of adhesion in their mesenchymal lineage-specific propagation. Nevertheless, the use of non-human supplements in culture (xeno-free issue) in addition to the lack of control over unknown factors in the serum hampers the clinical transition of MSCs. Read More

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http://dx.doi.org/10.1007/s12195-019-00604-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981336PMC
February 2020

Cas9 Protein Triggers Differential Expression of Inherent Genes Especially NGFR Expression in 293T Cells.

Cell Mol Bioeng 2020 Feb 2;13(1):61-72. Epub 2019 Dec 2.

College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108 China.

Introduction: CRISPR/CAS9 systems, which can be utilized biological experiments, have recently captured much attention for their important roles and benefits. However, full realization of the potential of CRISPR/CAS9 approaches requires addressing many challenges and side effects. The expression of genes and potential side effects of CRISPR/CAS9 in human cells remains to be elucidated. Read More

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http://dx.doi.org/10.1007/s12195-019-00606-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981332PMC
February 2020

Cell Communication Network Factor 4 (CCN4/WISP1) Shifts Melanoma Cells from a Fragile Proliferative State to a Resilient Metastatic State.

Cell Mol Bioeng 2020 Feb 17;13(1):45-60. Epub 2019 Oct 17.

Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26505 USA.

Introduction: Cellular communication network factor 4 (CCN4/WISP1) is a secreted matricellular protein that stimulates metastasis in multiple malignancies but has an unclear impact on phenotypic changes in melanoma. Recent data using cells edited a double-nickase CRISPR/Cas9 approach suggest that CCN4/WISP1 stimulates invasion and metastasis of melanoma cells. While these data also suggest that loss of CCN4/WISP1 increases cell proliferative, the CRISPR approach used may be an alternative explanation rather than the loss of gene function. Read More

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http://dx.doi.org/10.1007/s12195-019-00602-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981335PMC
February 2020

Differential Contributions of Actin and Myosin to the Physical Phenotypes and Invasion of Pancreatic Cancer Cells.

Cell Mol Bioeng 2020 Feb 31;13(1):27-44. Epub 2019 Oct 31.

Department of Integrative Biology and Physiology, University of California, 610 Charles E Young Dr. East, Los Angeles, CA 90095 USA.

Introduction: Metastasis is a fundamentally physical process in which cells deform through narrow gaps and generate forces to invade surrounding tissues. While it is commonly thought that increased cell deformability is an advantage for invading cells, we previously found that more invasive pancreatic ductal adenocarcinoma (PDAC) cells are stiffer than less invasive PDAC cells. Here we investigate potential mechanisms of the simultaneous increase in PDAC cell stiffness and invasion, focusing on the contributions of myosin II, Arp2/3, and formins. Read More

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http://dx.doi.org/10.1007/s12195-019-00603-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981337PMC
February 2020

Genome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function.

Cell Mol Bioeng 2020 Feb 25;13(1):17-26. Epub 2019 Sep 25.

Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, 1161 21st Ave South, S-3223 MCN, Nashville, TN 37232 USA.

Introduction: Bioengineering an implantable artificial kidney (IAK) will require renal epithelial cells capable of reabsorption of salt and water. We used genome engineering to modify cells for improved Na/H exchange and HO reabsorption. The non-viral transposon system enables genome engineering cells to stably overexpress one or more transgenes simultaneously. Read More

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http://dx.doi.org/10.1007/s12195-019-00601-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981331PMC
February 2020

Engineering of Exosomes to Target Cancer Metastasis.

Cell Mol Bioeng 2020 Feb 23;13(1):1-16. Epub 2019 Dec 23.

Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212 USA.

As a nanoscale subset of extracellular vehicles, exosomes represent a new pathway of intercellular communication by delivering cargos such as proteins and nucleic acids to recipient cells. Importantly, it has been well documented that exosome-mediated delivery of such cargo is involved in many pathological processes such as tumor progression, cancer metastasis, and development of drug resistance. Innately biocompatible and possessing ideal structural properties, exosomes offer distinct advantages for drug delivery over artificial nanoscale drug carriers. Read More

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http://dx.doi.org/10.1007/s12195-019-00607-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981329PMC
February 2020

Correction to: Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation.

Cell Mol Bioeng 2019 Oct 17;12(5):541-542. Epub 2019 Sep 17.

Toronto General Hospital Research Institute, University Health Network, 101 College St., MaRS, TMDT 3-904, Toronto, ON M5G 1L7 Canada.

[This corrects the article DOI: 10.1007/s12195-019-00574-3.]. Read More

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http://dx.doi.org/10.1007/s12195-019-00595-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816639PMC
October 2019

Cyclic Stretching of Fibrotic Microtissue Array for Evaluation of Anti-Fibrosis Drugs.

Cell Mol Bioeng 2019 Oct 28;12(5):529-540. Epub 2019 Aug 28.

Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY 14260 USA.

Introduction: Progression of pulmonary fibrosis, characterized by the deterioration of lung tissue's mechanical properties, is affected by respiratory motion-induced dynamic loading. Since the development of anti-fibrosis drugs faces major hurdles in animal tests and human clinical trials, preclinical models that can recapitulate fibrosis progression under physiologically-relevant cyclic loading hold great promise. However, the integration of these two functions has not been achieved in existing models. Read More

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http://dx.doi.org/10.1007/s12195-019-00590-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816662PMC
October 2019

Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization.

Cell Mol Bioeng 2019 Oct 24;12(5):511-528. Epub 2019 Sep 24.

Department of Biomedical Engineering, University of Wisconsin-Madison, 1550 Engineering Drive, Madison, WI 53706 USA.

Introduction: Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism by using light to control the nuclear localization of nuclease-dead Cas9, dCas9.

Methods: The dCas9 protein acts as a repressor for a gene of interest when localized to the nucleus in the presence of an appropriate guide RNA (sgRNA). Read More

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http://dx.doi.org/10.1007/s12195-019-00598-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816687PMC
October 2019

Dynamics of Non-Canonical Amino Acid-Labeled Intra- and Extracellular Proteins in the Developing Mouse.

Cell Mol Bioeng 2019 Oct 26;12(5):495-509. Epub 2019 Aug 26.

Weldon School of Biomedical Engineering, Purdue University, 206 South Martin Jischke Drive, West Lafayette, IN 47907 USA.

Introduction: Mapping protein synthesis and turnover during development will provide insight into functional tissue assembly; however, quantitative characterization has been hindered by a lack of tools. To address this gap, we previously demonstrated murine embryos can be labeled with the non-canonical amino acid azidohomoalanine (Aha), which enables the enrichment and identification of newly synthesized proteins. Using this technique, we now show how protein turnover varies as a function of both time and cellular compartment during murine development. Read More

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http://dx.doi.org/10.1007/s12195-019-00592-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816640PMC
October 2019

Coencapsulation of ISCs and MSCs Enhances Viability and Function of both Cell Types for Improved Wound Healing.

Cell Mol Bioeng 2019 Oct 1;12(5):481-493. Epub 2019 Jul 1.

Department of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854 USA.

Introduction: We previously demonstrated that insulin secreting cells (ISCs) accelerate healing of chronic wounds, and it is known that mesenchymal stem cells (MSCs) also accelerate wound healing. Here, we report that the combination of both cell types coencapsulated into a synthetic hydrogel dressing accelerates chronic wound healing 3 × faster than control and 2 × faster than each cell type delivered singly. Specifically, insulin released by ISCs activates the PI3/Akt pathway, which is vital to the function and survival of MSCs. Read More

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http://dx.doi.org/10.1007/s12195-019-00582-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816714PMC
October 2019

Tumor Cell Mechanosensing During Incorporation into the Brain Microvascular Endothelium.

Cell Mol Bioeng 2019 Oct 28;12(5):455-480. Epub 2019 Aug 28.

Fischell Department of Bioengineering, University of Maryland, College Park, College Park, MD 20742 USA.

Introduction: Tumor metastasis to the brain occurs in approximately 20% of all cancer cases and often occurs due to tumor cells crossing the blood-brain barrier (BBB). The brain microenvironment is comprised of a soft hyaluronic acid (HA)-rich extracellular matrix with an elastic modulus of 0.1-1 kPa, whose crosslinking is often altered in disease states. Read More

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http://dx.doi.org/10.1007/s12195-019-00591-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816707PMC
October 2019

Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation.

Cell Mol Bioeng 2019 Oct 21;12(5):443-454. Epub 2019 May 21.

Toronto General Hospital Research Institute, University Health Network, 101 College St., MaRS, TMDT 3-904, Toronto, ON M5G 1L7 Canada.

Introduction: The biggest bottleneck for cell-based regenerative therapy is the lack of a functional vasculature to support the grafts. This problem is exacerbated in diabetic patients, where vessel growth is inhibited. To address this issue, we aim to identify the causes of poor vascularization in 3D engineered tissues in diabetes and to reverse its negative effects. Read More

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http://dx.doi.org/10.1007/s12195-019-00574-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816716PMC
October 2019

Microparticle Depots for Controlled and Sustained Release of Endosomolytic Nanoparticles.

Cell Mol Bioeng 2019 Oct 3;12(5):429-442. Epub 2019 May 3.

Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN USA.

Introduction: Nucleic acids have gained recognition as promising immunomodulatory therapeutics. However, their potential is limited by several drug delivery barriers, and there is a need for technologies that enhance intracellular delivery of nucleic acid drugs. Furthermore, controlled and sustained release is a significant concern, as the kinetics and localization of immunomodulators can influence resultant immune responses. Read More

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http://dx.doi.org/10.1007/s12195-019-00571-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816657PMC
October 2019

Probing Single-Cell Mechanical Allostasis Using Ultrasound Tweezers.

Cell Mol Bioeng 2019 Oct 13;12(5):415-427. Epub 2019 Jun 13.

Department of Mechanical and Aerospace Engineering, New York University, Brooklyn, NY 11201 USA.

Introduction: In response to external stress, cells alter their morphology, metabolic activity, and functions to mechanically adapt to the dynamic, local environment through cell allostasis. To explore mechanotransduction in cellular allostasis, we applied an integrated micromechanical system that combines an 'ultrasound tweezers'-based mechanical stressor and a Förster resonance energy transfer (FRET)-based molecular force biosensor, termed "actinin-sstFRET," to monitor single-cell allostasis in response to transient stimulation in real time.

Methods: The ultrasound tweezers utilize 1 Hz, 10-s transient ultrasound pulses to acoustically excite a lipid-encapsulated microbubble, which is bound to the cell membrane, and apply a pico- to nano-Newton range of forces to cells through an RGD-integrin linkage. Read More

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http://dx.doi.org/10.1007/s12195-019-00578-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816625PMC
October 2019

Injectable, Hyaluronic Acid-Based Scaffolds with Macroporous Architecture for Gene Delivery.

Cell Mol Bioeng 2019 Oct 4;12(5):399-413. Epub 2019 Sep 4.

Department of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.

Introduction: Biomaterials can provide localized reservoirs for controlled release of therapeutic biomolecules and drugs for applications in tissue engineering and regenerative medicine. As carriers of gene-based therapies, biomaterial scaffolds can improve efficiency and delivery-site localization of transgene expression. Controlled delivery of gene therapy vectors from scaffolds requires cell-scale macropores to facilitate rapid host cell infiltration. Read More

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http://dx.doi.org/10.1007/s12195-019-00593-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816628PMC
October 2019

Cell Subtypes Within the Liver Microenvironment Differentially Interact with Lipid Nanoparticles.

Cell Mol Bioeng 2019 Oct 8;12(5):389-397. Epub 2019 May 8.

Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA USA.

Introduction: Lipid nanoparticles (LNPs) tend to accumulate in the liver due to physiological factors. Whereas the biological mechanisms that promote LNP delivery to hepatocytes have been reported, the mechanisms that promote delivery to other cell types within the liver microenvironment are poorly understood. Single cell profiling studies have recently identified subsets of Kupffer cells and hepatic endothelial cells with distinct gene expression patterns and biological phenotypes; we hypothesized these subtypes would differentially interact with nanoparticles. Read More

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http://dx.doi.org/10.1007/s12195-019-00573-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816632PMC
October 2019

Carrier-Free CXCR4-Targeted Nanoplexes Designed for Polarizing Macrophages to Suppress Tumor Growth.

Cell Mol Bioeng 2019 Oct 27;12(5):375-388. Epub 2019 Aug 27.

Department of Pharmaceutical Sciences, School of Pharmacy, University at Buffalo, The State University of New York, Buffalo, NY 14214 USA.

Introduction: Treatment options for cancer metastases, the primary cause of cancer mortality, are limited. The chemokine receptor CXCR4 is an attractive therapeutic target in cancer because it mediates metastasis by inducing cancer cell and macrophage migration. Here we engineered carrier-free CXCR4-targeting RNA-protein nanoplexes that not only inhibited cellular migration but also polarized macrophages to the M1 phenotype. Read More

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http://dx.doi.org/10.1007/s12195-019-00589-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816770PMC
October 2019

Dual Inhibitors-Loaded Nanotherapeutics that Target Kinase Signaling Pathways Synergize with Immune Checkpoint Inhibitor.

Cell Mol Bioeng 2019 Oct 21;12(5):357-373. Epub 2019 May 21.

Department of Chemical Engineering, University of Massachusetts, Amherst, MA USA.

Introduction: Immune checkpoint inhibitors that boost cytotoxic T cell-based immune responses have emerged as one of the most promising approaches in cancer treatment. However, it is increasingly being realized that T cell activation needs to be rationally combined with molecularly targeted therapeutics for a maximal anti-tumor outcome. Currently, two oncogenic drivers, MAPK and PI3K-mTOR have emerged as the two main molecular targets for combining with immunotherapy. Read More

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http://dx.doi.org/10.1007/s12195-019-00576-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816683PMC
October 2019

The 2019 Young Innovators of Cellular and Molecular Bioengineering.

Cell Mol Bioeng 2019 Oct 13;12(5):355-356. Epub 2019 Sep 13.

Department of Mechanical Engineering and Division of Medical Science, University of Victoria, Engineering Office Wing, Room 513, Victoria, BC Canada.

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http://dx.doi.org/10.1007/s12195-019-00599-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816748PMC
October 2019

Shaft Function of Kinesin-1's α4 Helix in the Processive Movement.

Cell Mol Bioeng 2019 Aug 25;12(4):345-354. Epub 2019 Jun 25.

Institute of Biophysics, Hebei University of Technology, Tianjin, 300401 China.

Introduction: Kinesin-1 motor is a molecular walking machine constructed with amino acids. The understanding of how those structural elements play their mechanical roles is the key to the understanding of kinesin-1 mechanism.

Methods: Using molecular dynamics simulations, we investigate the role of a helix structure, α4 (also called switch-II helix), of kinesin-1's motor domain in its processive movement along microtubule. Read More

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http://dx.doi.org/10.1007/s12195-019-00581-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816713PMC

Endothelial Cell Biomechanical Responses are Dependent on Both Fluid Shear Stress and Tensile Strain.

Cell Mol Bioeng 2019 Aug 9;12(4):311-325. Epub 2019 Jul 9.

Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794 USA.

Introduction: The goal of this study was to investigate how concurrent shear stress and tensile strain affect endothelial cell biomechanical responses.

Methods: Human coronary artery endothelial cells were exposed to concurrent pulsatile shear stress and cyclic tensile strain in a programmable shearing and stretching device. Three shear stress-tensile strain conditions were used: (1) pulsatile shear stress at 1 Pa and cyclic tensile strain at 7%, simulating normal stress/strain conditions in a healthy coronary artery; (2) shear stress at 3. Read More

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http://dx.doi.org/10.1007/s12195-019-00585-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816737PMC

Effects of Rolling-Sliding Mechanical Stimulation on Cartilage Preserved .

Cell Mol Bioeng 2019 Aug 9;12(4):301-310. Epub 2019 Jul 9.

College of Radiology, Shandong First Medical University&Shandong Academy of Medical Science, Taian, 271016 Shandong China.

Introduction: Mechanical stimulation is important for maintaining cartilage function. We used a loading device to exert rolling-sliding mechanical stimulation on cartilage preserved to investigate cartilage viability and the involved mechanisms.

Methods: Osteochondral grafts from pig knees were randomly classified into loading and control groups. Read More

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http://dx.doi.org/10.1007/s12195-019-00584-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816650PMC

Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.

Cell Mol Bioeng 2019 Aug 11;12(4):289-300. Epub 2019 Jul 11.

Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284 USA.

Introduction: Cell stretch is a method which can rapidly apply mechanical force through cell-matrix and cell-cell adhesions and can be utilized to better understand underlying biophysical questions related to intracellular force transmission and mechanotransduction.

Methods: 3D printable stretching devices suitable for live-cell fluorescent imaging were designed using finite element modeling and validated experimentally. These devices were then used along with FRET based nesprin-2G force sensitive biosensors as well as live cell fluorescent staining to understand how the nucleus responds to externally applied mechanical force in cells with both intact LINC (linker of nucleoskeleton and cytoskeleton) complex and cells with the LINC complex disrupted using expression of dominant negative KASH protein. Read More

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http://dx.doi.org/10.1007/s12195-019-00579-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816703PMC

Dynamic Model of Protease State and Inhibitor Trafficking to Predict Protease Activity in Breast Cancer Cells.

Cell Mol Bioeng 2019 Aug 19;12(4):275-288. Epub 2019 Jun 19.

Wallace H. Coulter Department of Biomedical Engineering at Georgia Institute of Technology & Emory University, 950 Atlantic Drive, Suite 3015, Atlanta, GA 30332 USA.

Introduction: Cysteine cathepsins are implicated in breast cancer progression, produced by both transformed epithelial cells and infiltrated stromal cells in tumors, but to date, no cathepsin inhibitor has been approved for clinical use due to unexpected side effects. This study explores cellular feedback to cathepsin inhibitors that might yield non-intuitive responses, and uses computational models to determine underlying cathepsin-inhibitor dynamics.

Methods: MDA-MB-231 cells treated with E64 were tested by multiplex cathepsin zymography and immunoblotting to quantify total, active, and inactive cathepsins S and L. Read More

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http://dx.doi.org/10.1007/s12195-019-00580-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816728PMC

Hierarchical-Clustering, Scaffold-Mining Exercises and Dynamics Simulations for Effectual Inhibitors Against Lipid-A Biosynthesis of .

Cell Mol Bioeng 2019 Jun 2;12(3):255-274. Epub 2019 May 2.

Bioinformatics Centre, Department of Bioinformatics, SVIMS University, Tirupati, 517507 AP India.

Introduction: Treatment failures of standard regimens and new strains egression are due to the augmented drug resistance conundrum. These confounding factors now became the drug designers spotlight to implement therapeutics against strains and to safeguard infected victims with devoid of adverse drug reactions. Thereby, to navigate the chemical space for medicine, paramount vital drug target opting considerations should be imperative. Read More

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http://dx.doi.org/10.1007/s12195-019-00572-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816686PMC
June 2019
1.318 Impact Factor

How Tumor Cells Can Make Use of Interstitial Fluid Flow in a Strategy for Metastasis.

Cell Mol Bioeng 2019 Jun 27;12(3):227-254. Epub 2019 Mar 27.

Department of Energy and Petroleum, University of Stavanger, 4068 Stavanger, Norway.

Introduction: The phenomenon of lymph node metastasis has been known for a long time. However, the underlying mechanism by which malignant tumor cells are able to break loose from the primary tumor site remains unclear. In particular, two competing fluid sensitive migration mechanisms have been reported in the experimental literature: (i) autologous chemotaxis (Shields et al. Read More

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http://dx.doi.org/10.1007/s12195-019-00569-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816778PMC

Mechanical Characterization of 3D Ovarian Cancer Nodules Using Brillouin Confocal Microscopy.

Cell Mol Bioeng 2019 Jun 7;12(3):215-226. Epub 2019 May 7.

Fischell Department of Bioengineering, University of Maryland, 4228 A. James Clark Hall, College Park, MD 20742 USA.

Introduction: The mechanical interaction between cells and their microenvironment is emerging as an important determinant of cancer progression and sensitivity to treatment, including in ovarian cancer (OvCa). However, current technologies limit mechanical analysis in 3D culture systems. Brillouin Confocal Microscopy is an optical non-contact method to assess the mechanical properties of biological materials. Read More

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http://dx.doi.org/10.1007/s12195-019-00570-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816613PMC

Nanomechanical Analysis of Extracellular Matrix and Cells in Multicellular Spheroids.

Cell Mol Bioeng 2019 Jun 30;12(3):203-214. Epub 2019 May 30.

Materials Science and Engineering Department, University of Connecticut, 97 North Eagleville Road, Unit 3136, Storrs, CT 06269-3136 USA.

Introduction: Over the last decade, atomic force microscopy (AFM) has played an important role in understanding nanomechanical properties of various cancer cell lines. This study is focused on Lewis lung carcinoma cell tumours as 3D multicellular spheroid (MS). Not much is know about the mechanical properties of the cells and the surrounding extracellular matrix (ECM) in rapidly growing tumours. Read More

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http://dx.doi.org/10.1007/s12195-019-00577-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816768PMC

Identifying Key Pathways and Components in Chemokine-Triggered T Lymphocyte Arrest Dynamics Using a Multi-Parametric Global Sensitivity Analysis.

Cell Mol Bioeng 2019 Jun 29;12(3):193-202. Epub 2019 May 29.

Department of Bioengineering, University of Pennsylvania, 240 Skirkanich Hall, 210 South 33rd St., Philadelphia, PA 19104 USA.

Introduction: The arrest of rolling T lymphocytes at specific locations is crucial to proper immune response function. We previously developed a model of chemokine-driven integrin activation, termed integrative signaling adhesive dynamics (ISAD). In addition, we have shown that loss of diacylglycerol kinase (DGK) leads to a gain of function regarding adhesion under shear flow. Read More

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http://dx.doi.org/10.1007/s12195-019-00575-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816696PMC

Modeling DNA Unwinding by AddAB Helicase-Nuclease and Modulation by Chi Sequences: Comparison with AdnAB and RecBCD.

Authors:
Ping Xie

Cell Mol Bioeng 2019 Apr 5;12(2):179-191. Epub 2018 Dec 5.

Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190 China.

Introduction: AddAB enzyme is a helicase-nuclease complex that initiates recombinational repair of double-stranded DNA breaks. It catalyzes processive DNA unwinding and concomitant resection of the unwound strands, which are modulated by the recognition of a recombination hotspot called Chi in the 3'-terminated strand. Despite extensive structural, biochemical and single molecule studies, the detailed molecular mechanism of DNA unwinding by the complex and modulation by Chi sequence remains unclear. Read More

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http://dx.doi.org/10.1007/s12195-018-00563-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816695PMC

Inference of Cellular Immune Environments in Sputum and Peripheral Blood Associated with Acute Exacerbations of COPD.

Cell Mol Bioeng 2019 Apr 15;12(2):165-177. Epub 2019 Mar 15.

Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 USA.

Introduction: Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death in the United States, with high associated costs. Most of the cost burden results from acute exacerbations of COPD (AE-COPD), events associated with heightened symptoms and mortality. Cellular mechanisms underlying AE-COPD are poorly understood, likely because they arise from dysregulation of complex immune networks across multiple tissue compartments. Read More

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http://dx.doi.org/10.1007/s12195-019-00567-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816611PMC

Differential Effects of the Antioxidants -Acetylcysteine and Pyrrolidine Dithiocarbamate on Mesenchymal Stem Cell Chondrogenesis.

Cell Mol Bioeng 2019 Apr 18;12(2):153-163. Epub 2019 Jan 18.

Department of Clinical Sciences, Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300W. Drake Road, Fort Collins, CO 80523 USA.

Introduction: Mesenchymal stem cell (MSC) chondrogenesis is associated with increases in intracellular reactive oxygen species (ROS), which may result in oxidative stress that is detrimental to cartilage regeneration. This study evaluated the ability of the antioxidants -acetylcysteine (NAC) or pyrrolidine dithiocarbamate (PDTC) to reduce intracellular ROS, and their effect on MSC chondrogenesis and maturation of cartilage-like extracellular matrix.

Methods: Equine bone marrow MSCs were cultured in serum-supplemented chondrogenic medium with or without NAC or PDTC. Read More

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http://dx.doi.org/10.1007/s12195-019-00566-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816680PMC

Announcing the Fourth Biomedical Engineering Education Summit Meeting.

Cell Mol Bioeng 2019 Apr 27;12(2):135-138. Epub 2019 Mar 27.

Vanderbilt University, Nashville, TN USA.

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http://dx.doi.org/10.1007/s12195-019-00568-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816753PMC

Correction to: Methods for 20S Immunoproteasome and 20S Constitutive Proteasome Determination Based on SPRI Biosensors.

Cell Mol Bioeng 2019 02 19;12(1):133. Epub 2018 Oct 19.

Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland.

[This corrects the article DOI: 10.1007/s12195-017-0478-7.]. Read More

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http://dx.doi.org/10.1007/s12195-018-00559-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816750PMC
February 2019

Correction to: Translating Mechanobiology to the Clinic: A Panel Discussion from the 2018 CMBE Conference.

Cell Mol Bioeng 2019 02 8;12(1):131. Epub 2018 Oct 8.

Columbia University, New York, NY USA.

[This corrects the article DOI: 10.1007/s12195-018-0556-5.]. Read More

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http://dx.doi.org/10.1007/s12195-018-00558-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816677PMC
February 2019

ROS and NO Dynamics in Endothelial Cells Exposed to Exercise-Induced Wall Shear Stress.

Cell Mol Bioeng 2019 Feb 8;12(1):107-120. Epub 2018 Oct 8.

School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian, 116024 China.

Introduction: Intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels are associated with vascular homeostasis and diseases. Exercise can modulate ROS and NO production through increasing frequency and magnitude of wall shear stress (WSS). However, the details of ROS and NO production in endothelial cells and their interplay under WSS induced by exercise at different intensities remain unclear. Read More

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http://dx.doi.org/10.1007/s12195-018-00557-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816775PMC
February 2019

Ciliotherapy Treatments to Enhance Biochemically- and Biophysically-Induced Mesenchymal Stem Cell Osteogenesis: A Comparison Study.

Cell Mol Bioeng 2019 Feb 20;12(1):53-67. Epub 2018 Nov 20.

Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College, Dublin, D02 R590 Ireland.

Introduction: New approaches to treat osteoporosis have focused on promoting bone formation through the targeting of osteoblasts and their progenitors, mesenchymal stem cells (MSCs). The primary cilium is a singular cellular extension known to play an important role in biochemical and biophysical osteogenic induction of MSCs. Defects in ciliary structure have been associated with a plethora of diseases. Read More

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http://dx.doi.org/10.1007/s12195-018-00561-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816767PMC
February 2019