5,879,852 results match your criteria Cellular & Molecular Biology Letters[Journal]

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Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population.

Clin Exp Dent Res 2020 Jul 11. Epub 2020 Jul 11.

Oral and Maxillofacial Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Background: Undifferentiated pleomorphic sarcomas are one of the most common subtypes of soft tissue sarcomas. These are aggressive mesenchymal tumors and are devoid of the major known biomarkers except vimentin. Our objective was to establish and characterize a primary cell population from a mandibular UPS specimen. Read More

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http://dx.doi.org/10.1002/cre2.301DOI Listing

Regenerative effects of human chondrocyte sheets in a xenogeneic transplantation model using immune-deficient rats.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan.

Although cell transplantation has attracted much attention in regenerative medicine, animal models continue to be used in translational research to evaluate safety and efficacy because cell sources and transplantation modalities are so diverse. In the present study, we investigated the regenerative effects of human chondrocyte sheets on articular cartilage in a xenogeneic transplantation model using immune-deficient rats. Osteochondral defects were created in the knee joints of immune-deficient rats that were treated as: Group A, untreated (without transplantation); Group B, transplantation of a layered chondrocyte sheet containing 5. Read More

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http://dx.doi.org/10.1002/term.3101DOI Listing

Two novel mutations in the MCM8 gene shared by two Chinese siblings with primary ovarian insufficiency and short stature.

Mol Genet Genomic Med 2020 Jul 11:e1396. Epub 2020 Jul 11.

Department of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: Minichromosome maintenance complex component 8 (MCM8) is responsible for homologous recombination and DNA double-strand breaks (DSBs) repair and is the cause of primary ovarian insufficiency (POI), which is seldom diagnosed in adolescents and children.

Methods: Whole-exome sequencing was performed in a 13-year-old girl, and Sanger sequencing was used to identify potentially pathogenic variants in her sister (aged 6 years and 7 months) and parents. To identify potential pathogenic mutations, DSBs were induced by mitomycin C (MMC), and the DNA repair capacity was evaluated by the histone H2AX phosphorylation level. Read More

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http://dx.doi.org/10.1002/mgg3.1396DOI Listing

Caffeine Upregulates Hepatic Sex Hormone-Binding Globulin Production by Increasing Adiponectin Through AKT/FOXO1 Pathway in White Adipose Tissue.

Mol Nutr Food Res 2020 Jul 11:e1901253. Epub 2020 Jul 11.

Diabetes and Metabolism Research Unit, Vall Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona and CIBERDEM (ISCIII), Barcelona, Spain.

Scope: Epidemiological studies have shown that caffeine increases serum sex hormone-binding globulin (SHBG) levels. The relationship between caffeine and SHBG production has never been studied before at molecular level. The aim of our study was to examine whether caffeine regulates SHBG production and to determine the associated molecular mechanisms. Read More

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http://dx.doi.org/10.1002/mnfr.201901253DOI Listing

Ageing-associated increase in SGLT2 disrupts mitochondrial/sarcoplasmic reticulum Ca homeostasis and promotes cardiac dysfunction.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Departments of Biophysics, Ankara University Faculty of Medicine, Ankara, Turkey.

The prevalence of death from cardiovascular disease is significantly higher in elderly populations; the underlying factors that contribute to the age-associated decline in cardiac performance are poorly understood. Herein, we identify the involvement of sodium/glucose co-transporter gene (SGLT2) in disrupted cellular Ca -homeostasis, and mitochondrial dysfunction in age-associated cardiac dysfunction. In contrast to younger rats (6-month of age), older rats (24-month of age) exhibited severe cardiac ultrastructural defects, including deformed, fragmented mitochondria with high electron densities. Read More

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http://dx.doi.org/10.1111/jcmm.15483DOI Listing

Enzyme Instructed Self-assembly of Naphthalimide-dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation.

Chem Asian J 2020 Jul 11. Epub 2020 Jul 11.

National Chiao Tung University, Materials Science and Engineering, 1001 University Rd., 30010, Hsinchu, TAIWAN.

Understanding the structure-morphology relationships of the self-assembled nanostructures is very crucial for developing the materials with the desired chemical and biological functions. Here, phosphate-based naphthalimide (NI) derivatives have been developed for the first time to study the enzyme instructed self-assembly process. Self-assembly of simple amino acid derivative NI-Y p resulted in non-specific amorphous aggregates in the presence of alkaline phosphatase enzyme. Read More

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http://dx.doi.org/10.1002/asia.202000575DOI Listing

MMP13 and TIMP1 are functional markers for two different potential modes of action by mesenchymal stem/stromal cells when treating osteoarthritis.

Stem Cells 2020 Jul 11. Epub 2020 Jul 11.

Institute of Lifecourse and Medical Sciences, University of Liverpool, UK.

Mesenchymal stem cells (MSCs) have been investigated as a potential injectable therapy for the treatment of knee osteoarthritis, with some evidence of success in preliminary human trials. However, optimisation and scale-up of this therapeutic approach depends on the identification of functional markers that are linked to their mechanism of action. One possible mechanism is through their chondrogenic differentiation and direct role in neo-cartilage synthesis. Read More

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http://dx.doi.org/10.1002/stem.3255DOI Listing

LINC00160 mediated paclitaxel-And doxorubicin-resistance in breast cancer cells by regulating TFF3 via transcription factor C/EBPβ.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Center for Precision Medicine, Anhui No.2 Provincial People's Hospital, Hefei, China.

Chemoresistance represents a major challenge in breast cancer (BC) treatment. This study aimed to probe the roles of LINC00160 in paclitaxel- and doxorubicin-resistant BC cells. Three pairs of BC and adjacent normal tissue were used for lncRNA microarray analysis. Read More

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http://dx.doi.org/10.1111/jcmm.15487DOI Listing

Organ care system lung resulted in lower apoptosis and inos expression in donor lungs.

Am J Transplant 2020 Jul 11. Epub 2020 Jul 11.

Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, Padova, Italy.

Ischemia/reperfusion injury after lung transplantation is still today an important complication in up to 25% of patients. The Organ Care System (OCS) Lung, an advanced normothermic ex-vivo lung perfusion system, was found to be effective in reducing primary graft dysfunction compared to standard organ care (SOC) but studies on tissue/molecular pathways that could explain these more effective clinical results are lacking. This observational longitudinal study aimed to investigate ischemia/reperfusion injury in 68 tissue specimens collected before and after reperfusion from 17 OCS and 17 SOC preserved donor lungs. Read More

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http://dx.doi.org/10.1111/ajt.16187DOI Listing

Knockdown of DNA binding protein A (dbpA) enhances the chemotherapy sensitivity of colorectal cancer via suppressing the Wnt/β-catenin/Chk1 pathway.

Cell Biol Int 2020 Jul 11. Epub 2020 Jul 11.

Department of hepatobiliary surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P.R. China.

DNA-binding protein A (dbpA) is reported to be upregulated in many cancers and associated with tumor progress. The present study aimed to investigate the role of dbpA in 5-Fluorouracil (5-FU)-resistant and Oxaliplatin (L-OHP)-resistant colorectal cancer (CRC) cells. We found that 5-FU and L-OPH treatment promoted the expression of dbpA. Read More

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http://dx.doi.org/10.1002/cbin.11416DOI Listing

Pyroglutamylated RFamide peptide 43: a putative modulator of testicular steroidogenesis.

Andrology 2020 Jul 11. Epub 2020 Jul 11.

Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.

Background: We have recently shown that QRFP and its receptor are predominantly expressed in germ cells, Sertoli cells, and Leydig cells in mice testes.

Objective: The present study investigated the role of QRFP in testicular steroidogenesis in mice.

Materials And Methods: Both ex vivo and in vivo experiments were performed. Read More

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http://dx.doi.org/10.1111/andr.12864DOI Listing

Acylglycerol kinase promotes the stemness of nasopharyngeal carcinoma cells by promoting β-catenin translocation to the nucleus through activating PI3K/Akt pathway.

Environ Toxicol 2020 Jul 11. Epub 2020 Jul 11.

Department of Otolaryngology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, China.

Recent evidences show that acylglycerol kinase (AGK) expression is related to the occurrence and development of various human cancers. However, its roles in nasopharyngeal carcinoma (NPC) progression are still unclear. This work aims to explore the roles of AGK in NPC cell stemness. Read More

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http://dx.doi.org/10.1002/tox.22994DOI Listing

Priming of microglia with IFN-γ impairs adult hippocampal neurogenesis and leads to depression-like behaviors and cognitive defects.

Glia 2020 Jul 11. Epub 2020 Jul 11.

School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.

Neuroinflammation driven by interferon-gamma (IFN-γ) and microglial activation has been linked to neurological disease. However, the effects of IFN-γ-activated microglia on hippocampal neurogenesis and behavior are unclear. In the present study, IFN-γ was administered to mice via intracerebroventricular injection. Read More

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http://dx.doi.org/10.1002/glia.23878DOI Listing

Administration of secretome from human placental stem cell conditioned media improves recovery of erectile function in the pelvic neurovascular injury model.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.

Introduction: Human placental stem cells (PSC) enhance histological and functional recovery in a rodent erectile dysfunction (ED) model. We tested the hypothesis that bioactive factors secreted by PSC (i.e. Read More

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http://dx.doi.org/10.1002/term.3105DOI Listing

Treatment of a large osseous defect in a feline tarsus using a stem cell-seeded custom implant.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Fitzpatrick Referrals, Eashing, Godalming, Surrey, United Kingdom.

Aim: To describe the treatment of an infected segmental bone defect in a cat using a novel, custom-designed titanium implant seeded with adipose-derived stem cells (AdMSCs) to facilitate osseous ingrowth and preserve limb function.

Case Report: Large bone defects occur secondary to trauma, infection, or neoplasia, and often result in amputation. We established a novel autologous AdMSC-impregnated trabecular metal spacer made using 3D printing, to bridge the distal tibia and metatarsal bones in the left pelvic limb of a cat that had previously undergone right pelvic limb amputation. Read More

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http://dx.doi.org/10.1002/term.3104DOI Listing

Clearance of therapy-induced senescent tumor cells by the senolytic ABT-263 via interference with BCL-X -BAX Interaction.

Mol Oncol 2020 Jul 11. Epub 2020 Jul 11.

Departments of Pharmacology & Toxicology, School of Medicine, Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, USA.

Tumor cells undergo senescence in response to both conventional and targeted cancer therapy. The induction of senescence in response to cancer therapy can contribute to unfavorable outcomes, potentially including cancer relapse. This work demonstrates that tumor cells induced into senescence by doxorubicin, etoposide, or radiation can give rise to viable tumors in vivo. Read More

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http://dx.doi.org/10.1002/1878-0261.12761DOI Listing

Disseminated Cryptococcosis with Cutaneous Manifestation.

Transpl Infect Dis 2020 Jul 11:e13412. Epub 2020 Jul 11.

Division of Infectious Diseases, Carolinas Medical Center, Atrium Health, Charlotte, NC, USA.

Cutaneous cryptococcosis is uncommon. It is usually a result of disseminated infection and can present with a wide of variety of skin lesions. We report a case of disseminated cryptococcosis in a kidney transplant recipient who presented with nodular lesions in the forehead following a bout of acute cellular rejection. Read More

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http://dx.doi.org/10.1111/tid.13412DOI Listing

Potentiation of calcium-activated chloride secretion and barrier dysfunction may underlie EGF receptor tyrosine kinase inhibitor-induced diarrhea.

Physiol Rep 2020 Jul;8(13):e14490

Division of Gastroenterology, Department of Medicine, University of California San Diego, La Jolla, CA, USA.

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFr TKIs) are first-line therapies for various cancers, and cause dose-limiting severe diarrhea in many patients. We hypothesized that diarrhea caused by EGFr TKIs might reflect actions on epithelial transport, barrier function, or both, which we tested using cell cultures including murine and human enteroid-derived monolayers (EDMs), analyzed using electrophysiological and other relevant methods. EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Read More

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http://dx.doi.org/10.14814/phy2.14490DOI Listing

Topical RT1640 treatment effectively reverses gray hair and stem cell loss in a mouse model of radiation-induced canities.

Pigment Cell Melanoma Res 2020 Jul 11. Epub 2020 Jul 11.

Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.

Gray hair is a visible sign of tissue degeneration during aging. Graying is attributed to dysfunction of melanocyte stem cells (McSCs) that results in depletion of their melanin-producing progeny. This non-lethal phenotype makes the hair follicle and its pigment system an attractive model for investigating mechanisms that contribute to tissue aging as well as therapeutic strategies to combat this process. Read More

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http://dx.doi.org/10.1111/pcmr.12913DOI Listing

De novo mutation and skewed X-inactivation in girl with BCAP31-related syndrome.

Hum Mutat 2020 Jul 11. Epub 2020 Jul 11.

Departments of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.

Full genome analysis of a young girl with deafness, dystonia, central hypomyelination, refractory seizure, and fluctuating liver function impairment revealed a heterozygous, de novo variant in the BCAP31 gene on chromosome Xq28 (NM_001256447.2:c.92G>A), mutations of which caused the X-linked recessive severe neurologic disorder DDCH (Deafness, Dystonia, and Cerebral Hypomyelination). Read More

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http://dx.doi.org/10.1002/humu.24080DOI Listing

Decellularised human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Laboratory of Experimental Ophthalmology, Department of Ophthalmology, Pius-Hospital, Carl von Ossietzky University, Germany.

The shortage of donor corneas as well as the limitations of tissue substitutes leads to the necessity to develop alternative materials for ocular surface reconstruction. Corneal surface substitutes must fulfill specific requirements such as high transparency, low immunogenicity, and mechanical stability combined with elasticity. This in vitro study evaluates a decellularized matrix secreted from human corneal fibroblast (hCF) as an alternative material for ocular surface reconstruction. Read More

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http://dx.doi.org/10.1002/term.3103DOI Listing

Low-magnitude vibration induces osteogenic differentiation of bone marrow mesenchymal stem cells via miR-378a-3p/Grb2 pathway to promote bone formation in a rat model of age-related bone loss.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

The dysfunction of bone marrow mesenchymal stem cells (BMSCs) in osteogenic differentiation is one of the main causes of age-related bone loss. Our previous studies have shown that low-magnitude vibration (LMV) induces the osteogenic differentiation of BMSCs derived from ovariectomized osteoporotic rats. To investigate whether LMV promotes osteogenic differentiation of BMSCs and its underlying mechanisms in aged rats, 20-month-old female Sprague-Dawley rats (n = 20) were randomly divided into LMV group (rats were vibrated at 0. Read More

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http://dx.doi.org/10.1096/fj.201902830RRRDOI Listing

MIB1-mediated degradation of WRN promotes cellular senescence in response to camptothecin treatment.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Department of Medical Genetics, Center for Medical Genetics, Peking University Health Science Center, Beijing, China.

Werner syndrome protein (WRN) plays critical roles in DNA replication, recombination, and repair, as well as transcription and cellular senescence. Ubiquitination and degradation of WRN have been reported, however, the E3 ubiquitin ligase of WRN is little known. Here, we identify mindbomb E3 ubiquitin protein ligase 1 (MIB1) as a novel E3 ubiquitin ligase for WRN protein. Read More

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http://dx.doi.org/10.1096/fj.202000268RRRDOI Listing

Nuclear β-catenin immunoexpression in scars.

J Cutan Pathol 2020 Jul 11. Epub 2020 Jul 11.

Department of Diagnostic Pathology, Shizuoka Cancer Center Hospital, Nagaizumi, Japan.

Background: Histopathologically, scars can mimic superficial fibromatoses. Superficial fibromatoses are known to show nuclear β-catenin immunoexpression, although the tumor types do not harbor CTNNB1 or APC alterations. The present study aimed to evaluate nuclear β-catenin immunoexpression in scars compared to that in superficial fibromatoses. Read More

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http://dx.doi.org/10.1111/cup.13806DOI Listing

MicroRNA-142 regulates osteoblast differentiation and apoptosis of mouse pre-osteoblast cells by targeting bone morphogenetic protein 2.

FEBS Open Bio 2020 Jul 11. Epub 2020 Jul 11.

Department of Anesthesiology, Weifang People's Hospital, Weifang, Shandong Province, 261041, P. R. China.

Osteoporosis is a common disease that can seriously impair the physical and mental health of the elderly. However, current treatment is unsatisfactory, due to the lack of effective therapeutic targets. Abnormal expression and involvement of microRNA-142 (miR-142) have been identified in many diseases, including bone-related diseases. Read More

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http://dx.doi.org/10.1002/2211-5463.12929DOI Listing

A COP1-PIF-HEC regulatory module fine-tunes photomorphogenesis in Arabidopsis.

Plant J 2020 Jul 11. Epub 2020 Jul 11.

Department of Molecular Biosciences, The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX, 78712, USA.

Light responses mediated by the photoreceptors play crucial roles in regulating different aspects of plant growth and development. An E3 ubiquitin ligase complex CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1)1/SUPPRESSOR OF PHYA (SPA), one of the central repressors of photomorphogenesis, is critical for maintaining skotomorphogenesis. It targets several positive regulators of photomorphogenesis for degradation in darkness. Read More

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http://dx.doi.org/10.1111/tpj.14908DOI Listing

DNMT3B deficiency alters mitochondrial biogenesis and α-ketoglutarate levels in human embryonic stem cells.

Stem Cells 2020 Jul 11. Epub 2020 Jul 11.

Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo and Oslo University Hospital, Oslo, Norway.

Embryonic stem cell (ESC) renewal and differentiation is regulated by metabolites that serve as co-factors for epigenetic enzymes. Increase of α-ketoglutarate (α-KG), a co-factor for histone and DNA demethylases, triggers multi-lineage differentiation in human ESCs. To gain further insight how the metabolic fluxes in pluripotent stem cells can be influenced by inactivating mutations in epigenetic enzymes, we generated human ESCs deficient for de novo DNA methyltransferases (DNMT) 3A and 3B. Read More

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http://dx.doi.org/10.1002/stem.3256DOI Listing

A cholesterosis of the common bile duct successfully diagnosed macroscopically and histologically using the transpapillary approach.

Dig Endosc 2020 Jul 11. Epub 2020 Jul 11.

Division of Gastroenterology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8575, Japan.

Cholesterosis is defined as the aggregation of foamy cells under the mucosa [1]. Although cholesterosis of the gallbladder is a common disease, cholesterosis of the common bile duct (CBD) is rare, with only <40 cases reported [2], and diagnosis is extremely difficult [3]. Since cholesterosis is basically a benign tumor, a direct visual examination and histopathological diagnosis is important to reduce unnecessary surgery [4], and endoscopic retrograde cholangiopancreatography (ERCP) and its related procedure have great benefits for patients because of its less-invasiveness and simplicity. Read More

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http://dx.doi.org/10.1111/den.13790DOI Listing

Elevated In Vitro Kinase Activity in Peripheral Blood Mononuclear Cells of Leucine-Rich Repeat Kinase 2 G2019S Carriers: A Novel Enzyme-Linked Immunosorbent Assay-Based Method.

Mov Disord 2020 Jul 11. Epub 2020 Jul 11.

Division of Basic Neurosciences, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.

Background: Leucine-rich repeat kinase 2 kinase inhibitors are being vigorously pursued as potential therapeutic options; however, there is a critical need for sensitive and quantitative assays of leucine-rich repeat kinase 2 function and target engagement.

Objectives: Our objective was to compare collection and storage protocols for peripheral blood mononuclear cells, and to determine the optimal conditions for downstream analyses of leucine-rich repeat kinase 2 in PD cohorts.

Methods: Here, we describe enzyme-linked immunosorbent assay-based assays capable of detecting multiple aspects of leucine-rich repeat kinase 2 function at endogenous levels in human tissues. Read More

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http://dx.doi.org/10.1002/mds.28175DOI Listing

Aldosterone-induced microRNAs act as feedback regulators of mineralocorticoid receptor signaling in kidney epithelia.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA.

The final steps in the Renin-Angiotensin-Aldosterone signaling System (RAAS) involve binding of the corticosteroid hormone, aldosterone to its mineralocorticoid receptor (MR). The bound MR interacts with response elements to induce or repress the transcription of aldosterone-regulated genes. A well characterized aldosterone-induced gene is the serum and glucocorticoid-induced kinase (SGK1), which acts downstream to increase sodium transport in distal kidney nephron epithelial cells. Read More

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http://dx.doi.org/10.1096/fj.201902254RRDOI Listing

Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A).

Hum Mutat 2020 Jul 11. Epub 2020 Jul 11.

Brain and Mitochondrial Research Group, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia.

Defects in the motor domain of kinesin family member 1A (KIF1A), a neuron-specific ATP-dependent anterograde axonal transporter of synaptic cargo, are well-recognized to cause a spectrum of neurological conditions, commonly known as KIF1A-associated neurological disorders (KAND). Here we report one mutation-negative female with classic Rett syndrome (RTT) harboring a de novo heterozygous novel variant [NP_001230937.1:p. Read More

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http://dx.doi.org/10.1002/humu.24079DOI Listing

Epithelial/mesenchymal heterogeneity of high-grade serous ovarian carcinoma samples correlates with miRNA let-7 levels and predicts tumor growth and metastasis.

Mol Oncol 2020 Jul 11. Epub 2020 Jul 11.

Department of Basic Sciences, Division of Biochemistry, Loma Linda University, Loma Linda, CA, USA.

Patient-derived samples present an advantage over current cell line models of high grade serous ovarian cancer (HGSOC) that are not always reliable and phenotypically faithful models of in vivo HGSOC. To improve upon cell line models of HGSOC, we set out to characterize a panel of patient-derived cells and determine their epithelial and mesenchymal characteristics. We analyzed RNA and protein expression levels in patient-derived xenograft (PDX) models of HGSOC, and functionally characterized these models using flow cytometry, wound healing assays, invasion assays and spheroid cultures. Read More

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http://dx.doi.org/10.1002/1878-0261.12762DOI Listing

Tissue-resident Mucosal-associated invariant T (MAIT) cells in the human kidney represent a functionally distinct subset.

Eur J Immunol 2020 Jul 11. Epub 2020 Jul 11.

Amsterdam UMC, University of Amsterdam, Division of Internal Medicine, Department of Nephrology, Renal Transplant Unit, Amsterdam Infection & Immunity Institute (AI&II), Meibergdreef 9, Amsterdam, The Netherlands.

Mucosal-associated invariant T (MAIT) cells are innate-like T-cells that recognize bacterial riboflavin metabolites. They are present in human blood but are abundant at barrier sites including the liver, lungs, and kidneys, where they possess a CD69 /CD103 tissue-resident phenotype. In renal tissue, MAIT cells likely defend against the ascending uropathogens responsible for urinary tract infections (UTIs), which are common, especially among renal transplant recipients (RTRs). Read More

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http://dx.doi.org/10.1002/eji.202048644DOI Listing

Reduced expression of the glucocorticoid receptor in the hippocampus of patients with drug-resistant temporal lobe epilepsy and comorbid depression.

Epilepsia 2020 Jul 11. Epub 2020 Jul 11.

Amsterdam UMC, University of Amsterdam, Department of (Neuro) Pathology, Amsterdam Neuroscience, Amsterdam, the Netherlands.

Objective: Depressive disorders are common among about 50% of the patients with drug-resistant temporal lobe epilepsy (TLE). The underlying etiology remains elusive, but hypothalamus-pituitary-adrenal (HPA) axis activation due to changes in glucocorticoid receptor (GR) protein expression could play an important role. Therefore, we set out to investigate expression of the GR in the hippocampus, an important brain region for HPA axis feedback, of patients with drug-resistant TLE, with and without comorbid depression. Read More

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http://dx.doi.org/10.1111/epi.16598DOI Listing

The impact of damage-associated molecular patterns on the neurotransmitter release and gene expression in the ex vivo rat carotid body.

Exp Physiol 2020 Jul 11. Epub 2020 Jul 11.

Department of Physiology and Pharmacology, Section for Anesthesiology and Intensive Care Medicine, Karolinska Institutet, Stockholm, Sweden.

What is the central question of this study? Testing the hypothesis that carotid bodies (CBs) are modulated by the damage-associated molecular patterns (DAMPs), humoral factors of aseptic tissue injury. What are the main findings and their importance? DAMPs, (HMGB1, S100 A8/A9) and blood plasma from the rats subjected to tibia surgery, a model of aseptic injury, stimulate the release of neurotransmitters (ATP, dopamine) and TNF-a from ex vivo rat CBs. CB RNA sequencing data indicate all-thiol HMGB1-mediated upregulation of immune-related biological pathways. Read More

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http://dx.doi.org/10.1113/EP088705DOI Listing

Molecular and functional characterization of urine-derived podocytes from patients with Alport syndrome.

J Pathol 2020 Jul 11. Epub 2020 Jul 11.

Department of Molecular Biotechnology and Health Sciences, University of Torino, Italy.

Alport syndrome (AS) is a genetic disorder involving mutations in the genes encoding collagen IV α3, α4 or α5 chains, resulting in the impairment of glomerular basement membrane. Podocytes are responsible for production and correct assembly of collagen IV isoforms; however, data on the phenotypic characteristics of human AS podocytes and their functional alterations are currently limited. The evident loss of viable podocytes into the urine of patients with active glomerular disease enables their isolation in a non-invasive way. Read More

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http://dx.doi.org/10.1002/path.5496DOI Listing

The E3 ubiquitin ligase Itch deficiency promotes antigen-driven B-cell responses in mice.

Eur J Immunol 2020 Jul 11. Epub 2020 Jul 11.

Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, 100069, China.

Deficiency of Itch, an E3 ubiquitin ligase, usually induced severe systemic and progressive autoimmune disease. The Itch function is well-studied in T cells but not in B cells. We hypothesize that B-cell-specific Itch deficiency promoted antigen-induced B-cell activation and antibody-expressing plasma cell (PC) production. Read More

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http://dx.doi.org/10.1002/eji.202048640DOI Listing

Functionally Distinct IFNγ IL-17A Th cells in experimental autoimmune uveitis: T cell heterogeneity, migration and steroid response.

Eur J Immunol 2020 Jul 11. Epub 2020 Jul 11.

UCL Institute of Ophthalmology, University College London, London, UK.

Immunopathogenic roles for both Th1 (CD4 IFNγ ) and Th17 (CD4 IL-17A ) cells have been demonstrated in experimental autoimmune uveitis (EAU). However, the role for Th17/Th1 (CD4 T cells co-expressing IFNγ and IL-17A) cells in EAU is not yet understood. Using interphotoreceptor retinoid-binding protein (IRBP) peptide-induced EAU in mice, we found increased levels of Th17/Th1 cells in EAU retinae (mean 9. Read More

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http://dx.doi.org/10.1002/eji.202048616DOI Listing

Varying Presentations and Favourable Outcomes of COVID-19 Infection in Children and Young Adults with Sickle Cell Disease: An Additional Case Series with Comparisons to Published Cases.

Br J Haematol 2020 Jul 11. Epub 2020 Jul 11.

Divisions of Hematology/ Oncology and Cellular Therapy, Cohen Children's Medical Center, New York, NY, 11040, United States.

Patients with sickle cell disease (SCD) are theoretically at greater risk for serious illness from COVID 19 due to the underlying pathophysiology of chronic inflammation with an increased risk of thrombosis, especially at the time of a vaso-occlusive event (1). Individuals living with SCD are also considered to be immunocompromised due to autoinfarction of their spleen or surgical splenectomy, often as a result of red cell splenic sequestration (2). In addition, patients with SCD often have comorbidities and secondary organ dysfunction that can put them at risk of significant morbidity and or mortality from the severe acute respiratory coronavirus 2 (SARS-CoV-2), and its subsequent disease COVID-19. Read More

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http://dx.doi.org/10.1111/bjh.17013DOI Listing

Bcl-3 inhibits lupus-like phenotypes in BL6/lpr mice.

Eur J Immunol 2020 Jul 11. Epub 2020 Jul 11.

Laboratory of Molecular Immunology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.

Bcl-3 is an atypical member of the IκB family that modulates NF-κB activity in nuclei. lpr mice carry the lpr mutation in Fas, resulting in functional loss of this death receptor; they serve as models for lupus erythematosus and ALPS. To explore the biologic roles of Bcl-3 in this disease model we generated BL6/lpr mice lacking Bcl-3. Read More

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http://dx.doi.org/10.1002/eji.202048584DOI Listing

Clinical characteristics and outcome of SARS-CoV-2 infection in Italian pediatric oncology patients: a study from the Infectious Diseases Working Group of the AIEOP.

J Pediatric Infect Dis Soc 2020 Jul 11. Epub 2020 Jul 11.

Pediatric Hematology Oncology, Department of Mother and Child, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy.

Background: Little is known as yet about the outcome of SARS-CoV-2 infection in children being treated for cancer.

Methods: We collected information on the clinical characteristics and outcomes of a cohort of 29 children (16 females and 13 males, median age 7 years, range [0-16]) diagnosed with SARS-CoV-2 infection while on chemotherapy/immunotherapy (N=26), or after stem cell transplantation (N=3) during the peak of the epidemic in Italy. These patients suffered from leukemia (N=16), lymphoma (N=3), solid tumors (N=10), and Langerhans cell histiocytosis (N=1). Read More

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http://dx.doi.org/10.1093/jpids/piaa088DOI Listing

polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice.

Aging (Albany NY) 2020 Jul 11;12. Epub 2020 Jul 11.

Department of Pathophysiology, School of Basic Medicine, Key Laboratory of Education Ministry of China for Neurological Disorders, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Polysaccharides (CPPs), a traditional Chinese medicine used for thousands of years, is a potential neuroprotective polysaccharide via a relatively poorly understood mechanism. We previously reported that CPPs attenuated tau pathology in hTau transfected mice and therefore in the current work investigated the effect of CPPs on Aβ toxicity and cognitive defects in APP/PS1 mice model. It was found that one-month intragastric administration of CPPs significantly ameliorated cognitive defects in APP/PS1 mice. Read More

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http://dx.doi.org/10.18632/aging.103445DOI Listing

Penehyclidine hydrochloride inhibits renal ischemia/reperfusion-induced acute lung injury by activating the Nrf2 pathway.

Aging (Albany NY) 2020 Jul 11;12. Epub 2020 Jul 11.

Department of Anesthesiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

The nuclear factor (NF)-κB and NOD-like receptor protein 3 (NLRP3) pathways promote inflammatory signaling that injures the kidneys, whereas the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway promotes anti-inflammatory signaling that inhibits oxidative damage. Penehyclidine hydrochloride (PHC) inhibits NF-κB and activates Nrf2 signaling. We investigated whether PHC induces communication between the Nrf2 and NF-κB/NLRP3 pathways, thereby protecting against renal ischemia/reperfusion (rI/R)-induced lung inflammation. Read More

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http://dx.doi.org/10.18632/aging.103444DOI Listing

Protein and calorie restriction may improve outcomes in living kidney donors and kidney transplant recipients.

Aging (Albany NY) 2020 Jul 11;12. Epub 2020 Jul 11.

Department of Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.

Previously, we and others showed that dietary restriction protects against renal ischemia-reperfusion injury in animals. However, clinical translation of preoperative diets is scarce, and in the setting of kidney transplantation these data are lacking. In this pilot study, we investigated the effects of five days of a preoperative protein and caloric dietary restriction (PCR) diet in living kidney donors on the perioperative effects in donors, recipients and transplanted kidneys. Read More

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http://dx.doi.org/10.18632/aging.103619DOI Listing

A cellular and attentional network explanation of consciousness.

Authors:
Gonzalo Munévar

Conscious Cogn 2020 Jul 8;83:102982. Epub 2020 Jul 8.

Lawrence Technological University, United States. Electronic address:

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http://dx.doi.org/10.1016/j.concog.2020.102982DOI Listing

Splenectomy enhances the Ly6C phenotype in hepatic macrophages by activating the ERK1/2 pathway during liver fibrosis.

Int Immunopharmacol 2020 Jul 7;86:106762. Epub 2020 Jul 7.

National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China; The Liver and Spleen Diseases Research Center, Xi'an, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, China. Electronic address:

Background And Aim: Splenectomy has been reported to attenuate liver fibrosis. In addition, phenotype transitions of infiltrating macrophages, including Ly6C and Ly6C, play an essential role in the liver fibrosis. However, whether the spleen can regulate the phenotype switch of macrophages and the underlying mechanism still remain unclear. Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106762DOI Listing

Inhibition of miR-93 promotes interferon effector signaling to suppress influenza A infection by upregulating JAK1.

Int Immunopharmacol 2020 Jul 8;86:106754. Epub 2020 Jul 8.

Institute of Organ Transplantation, Changzhen Hospital, Navy Medical University, Shanghai, China. Electronic address:

Type I interferons play a critical role in host defense against influenza virus infection. Interferon cascade induces the expression of interferon-stimulated genes then subsequently promotes antiviral immune responses. The microRNAs are important regulators of innate immunity, but microRNAs-mediated regulation of interferon cascade during influenza infection remains to be fully identified. Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106754DOI Listing

Transitional immature regulatory B cells and regulatory cytokines can discriminate chronic antibody-mediated rejection from stable graft function.

Int Immunopharmacol 2020 Jul 8;86:106750. Epub 2020 Jul 8.

Department of Immunology, School of Medicine, Tehran University of Medical Science, Tehran, Iran. Electronic address:

Background: The balance between inflammatory and anti-inflammatory responses of the immune system has been demonstrated to determine the fate of transplanted allografts. Here we analyzed CD19CD24CD38 immature transitional regulatory B (TRB) cells, as well as the gene and protein levels of interleukin (IL)-10 and transforming growth factor (TGF)-β in the three separate groups, include of stable transplanted subjects, chronic antibody-mediated rejection (cAMR) patients, and healthy individuals.

Method: Peripheral blood mononuclear cells (PBMCs) from stable subjects (n = 36), cAMR patients (n = 36) and healthy controls (n = 18) were isolated. Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106750DOI Listing

A model based on tumor-infiltrating immune cells for predicting the relapse rates of patients with testicular germ cell tumors.

Int Immunopharmacol 2020 Jul 8;86:106710. Epub 2020 Jul 8.

Department of Urology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China. Electronic address:

Objective: The activities of tumor-infiltrating immune cells (TIICs) play an important role in the outcomes of many types of cancers. Here, we sought to describe the landscape of TIICs in testicular germ cell tumors (TGCT) and to develop a prognostic model based on this information.

Methods: The Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) algorithm was used to determine the proportions of 22 types of TIICs in a TGCT dataset (n = 74). Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106710DOI Listing

Regulation of mucin 1 expression and its relationship with oral diseases.

Arch Oral Biol 2020 Jul 1;117:104791. Epub 2020 Jul 1.

Department of Oral Diagnostic Sciences, Institute of Dentistry, Faculty of Health Sciences, University of Eastern Finland, Kuopio Campus, Finland. Electronic address:

Objective: The aim of this study is to describe the polymorphic mucin 1 (MUC1), and to provide an overview of the known complex and multiple functions of MUC1 in normal oral mucosa and oral mucosal lesions in compromised situations as well as exploring the challenges associated with the heterogeneous nature of MUC1. We will review the current knowledge and provide insights into the future management possibilities of using MUC1 as a therapeutic agent.

Methods: A literature search of the electronic databases included MEDLINE (1966 -December 2019) and hand searches of cross-references were undertaken using terms related to mucins, MUC1. Read More

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http://dx.doi.org/10.1016/j.archoralbio.2020.104791DOI Listing
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