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    1321 results match your criteria Cells tissues organs[Journal]

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    Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization.
    Cells Tissues Organs 2018 May 9:1-13. Epub 2018 May 9.
    Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cell Therapy, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
    Introduction: Diabetes is known as a worldwide disease with a great burden on society. Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered. Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells. Read More

    Innervation of the Human Incisive Papilla: Comparison with Other Oral Regions.
    Cells Tissues Organs 2018 May 7:1-12. Epub 2018 May 7.
    Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, Japan.
    Immunohistochemistry for several neurochemical substances was performed on the human incisive papilla and other oral structures. Sodium channel alpha subunit 7 (SCN7A) protein-immunoreactive (IR) Schwann cells and protein gene product 9.5 (PGP 9. Read More

    Immunolocalization of Receptor and Chemoreceptor Modules in the Sheep Vomeronasal Organ.
    Cells Tissues Organs 2018 Apr 19. Epub 2018 Apr 19.
    The vomeronasal organ (VNO) is the peripheral receptor organ of the accessory olfactory system, which is responsible for both sexual and innate behaviors. The degree of neuronal differentiation and maturation of the vomeronasal receptor cells together with the verification of the presence of the solitary chemoreceptor cells (SCCs) in the VNO of Corriedale sheep were assessed using immunofluorescence. A protein gene product 9. Read More

    The Role of Epithelial Stat3 in Amelogenesis during Mouse Incisor Renewal.
    Cells Tissues Organs 2018 Mar 16. Epub 2018 Mar 16.
    Program in Craniofacial Biology and Department of Orofacial Sciences, University of California San Francisco (UCSF), San Francisco, California, USA.
    The aim of this study was to evaluate the role of epithelial signal transducer and activator of transcription 3 (STAT3) in mouse incisor amelogenesis. Since Stat3 is expressed in the epithelial component of developing and adult mouse teeth, we generated and analyzed Krt14Cre/+;Stat3fl/fl mutant mice in which Stat3 was inactivated in epithelia including ameloblast progenitors and ameloblasts, the cells responsible for enamel formation. Histological analysis showed little enamel matrix in mutant incisors compared to controls. Read More

    Functional Morphology of Thecal Glands in the Ovary of Japanese Quails (Coturnix japonica).
    Cells Tissues Organs 2018 1;205(1):32-41. Epub 2018 Mar 1.
    The role of thecal glands in the ovary of birds remains controversial. Using transmission electron microscopy and immunohistochemistry, immunohistochemical localisation of cyclooxygenase I and II (COX-1 and COX-2), oestrogen receptor α and β (ER-α and ER-β), androgen receptor (AR) and progesterone receptor (PR), a detailed analysis of the thecal glands was performed. Our ultrastructural studies revealed that the thecal glands of the quail ovary consist of 2 cell types, steroid-producing cells (SPCs) and enclosing cells (ENCs). Read More

    Osteoconduction of Unrestricted Somatic Stem Cells on an Electrospun Polylactic-Co-Glycolic Acid Scaffold Coated with Nanohydroxyapatite.
    Cells Tissues Organs 2018 8;205(1):9-19. Epub 2018 Feb 8.
    Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
    The limitation of traditional bone grafts could be overcome by applying engineered bone constructs, which are mainly produced by seeding suitable stem cells on appropriate scaffolds. So far, bone marrow-derived stromal cells have been the most applied cells in bone tissue engineering, but current data show that unrestricted somatic stem cells (USSCs) from human cord blood might actually be a better stem cell source due to the accessibility and noninvasive procedure of collection. In this study, we cultured USSCs on a plasma-treated electrospun polylactic-co-glycolic acid (PLGA) scaffold coated with nanohydroxyapatite (nHA). Read More

    Ultramorphological Characteristics of Podocyte Development in the Human Fetal Metanephros.
    Cells Tissues Organs 2018 7;205(1):42-52. Epub 2018 Feb 7.
    Medical Faculty, University of Nis, Nis, Serbia.
    The aim of this study was to determine the developmental characteristics of podocytes in the human fetal metanephros using scanning electron microscopy, light microscopy, and transmission electron microscopy. Kidney samples of 15 human fetuses of both sexes (gestational age 10-22 weeks) were analyzed. At the S-shaped body stage, primitive podocytes were arranged in a layer of cuboidal cells beneath the vascular cleft. Read More

    Responsiveness of Infrapatellar Fat Pad Volume Change to Body Weight Loss or Gain: Data from the Osteoarthritis Initiative.
    Cells Tissues Organs 2018 1;205(1):53-62. Epub 2018 Feb 1.
    Obesity is a potent risk factor for knee osteoarthritis (OA) that is driven by mechanical and potentially endocrine mechanisms, and it affects women more frequently than men. The infrapatellar fat pat (IPFP) represents a potential link between obesity, intra-articular inflammation and structural pathology. Here we investigate whether the IPFP is responsive to body weight loss/gain in women and how its responsiveness to weight change compares to that of subcutaneous fat (SCF) of the thigh. Read More

    Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair.
    Cells Tissues Organs 2018 1;205(1):20-31. Epub 2018 Feb 1.
    The combination of bone tissue scaffolds with osteogenic induction factors is an effective strategy to facilitate bone healing processes. Here, chitosan (CS)/nano-hydroxyapatite (HA) scaffolds containing simvastatin (SIM)-loaded PLGA microspheres were fabricated by combining a freeze-drying technique with a modified water-oil-water emulsion method. The CS/HA weight ratio of 1:2 was selected by analyzing the effect of HA content on the micro-architecture, mechanical strength, and biocompatibility of the scaffold. Read More

    Expression Profile of NOTCH3 in Mouse Spermatogonia.
    Cells Tissues Organs 2017 22;204(5-6):283-292. Epub 2017 Jan 22.
    Laboratory of Molecular Embryology, Department of Genome System Science, Yokohama City University, Yokohama, Japan.
    Stable and sustainable spermatogenesis is supported by the strict regulation of self-renewal and differentiation of spermatogonial stem cells (SSC), which are a rare population of undifferentiated spermatogonia. It has been revealed that some signaling factors regulate the self-renewal of SSC; however, the molecular mechanism of SSC maintenance is still not completely understood. Notch signaling is an evolutionarily conserved juxtacrine signaling that plays important roles in the cell fate determination of various tissue stem cells. Read More

    Complete Static Repopulation of Decellularized Porcine Tissues for Heart Valve Engineering: An in vitro Study.
    Cells Tissues Organs 2017 8;204(5-6):270-282. Epub 2017 Nov 8.
    Department of Basic Medical Sciences, Ghent University, Ghent, Belgium.
    To date, a completely in vitro repopulated tissue-engineered heart valve has not been developed. This study focused on sequentially seeding 2 cell populations onto porcine decellularized heart valve leaflets (HVL) and pericardia (PER) to obtain fully repopulated tissues. For repopulation of the interstitium, porcine valvular interstitial cells (VIC) and bone marrow-derived mesenchymal stem cells (BM-MSC) or adipose tissue-derived stem cells (ADSC) were used. Read More

    Immunohistochemical Localization of Calbindin D28k, Parvalbumin, and Calretinin in the Superior Olivary Complex of Circling Mice.
    Cells Tissues Organs 2017 4;204(5-6):304-313. Epub 2017 Nov 4.
    Department of Pharmacology, Dankook University College of Medicine, Cheonan, South Korea.
    The circling mouse serves as a hearing loss model. It has spontaneous tmie gene mutations that cause hair cell and cochlear degeneration. However, little is known about the role of the tmie gene in superior olivary complex (SOC) regions, in which sound information from the two ears is integrated and primarily relayed to the nuclei of the lateral lemniscus and inferior colliculus. Read More

    Changes in the Proliferation/Apoptosis Balance in the Bovine Ovary: A Key Early Event in Follicular Persistence.
    Cells Tissues Organs 2017 3;204(5-6):314-325. Epub 2017 Nov 3.
    Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral), Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Esperanza, Argentina.
    The objective of this work was to evaluate proliferation and apoptosis in the bovine ovary in a model of follicular persistence induced by low levels of progesterone to detect incipient changes during cystic ovarian disease development on the expected day of ovulation (day 0) and after 5, 10, and 15 days of follicular persistence. We analyzed cell proliferation by evaluating the expression of Ki-67 and apoptosis by evaluating caspase-3, BAX, and BCL2 expression. Proliferation was similar in the granulosa and theca cells of antral follicles in the P0 group (treated with progesterone up to the expected day of ovulation) and in the control group. Read More

    Avian Collagen Is Useful for the Construction of Skin Equivalents.
    Cells Tissues Organs 2017 21;204(5-6):261-269. Epub 2017 Oct 21.
    Department of Biochemistry, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
    As a result of restrictions on animal experimentation, improved skin equivalents (SEs) are needed as alternative test models. This work investigated the effects of avian collagen on the construction of SEs, and to the best of our knowledge is the first study to do so. Hematoxylin and eosin and immunohistochemical staining were used to analyze the SEs. Read More

    A Novel Method to Induce Cartilage Regeneration with Cubic Microcartilage.
    Cells Tissues Organs 2017 4;204(5-6):251-260. Epub 2017 Oct 4.
    Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Kindai University, Osaka-sayama, Japan.
    Cartilage tissue is characterized by its poor regenerative properties, and the clinical performance of cartilage grafts to replace cartilage defects has been unsatisfactory. Recently, cartilage regeneration with mature chondrocytes and stem cells has been developed and applied in clinical settings. However, there are challenges with the use of mature chondrocytes and stem cells for tissue regeneration, including the high costs associated with the standard stem cell isolation methods and the decreased cell viability due to cell manipulation. Read More

    The Peritoneum: Health, Disease, and Perspectives regarding Tissue Engineering and Cell Therapies.
    Cells Tissues Organs 2017 4;204(5-6):211-217. Epub 2017 Oct 4.
    Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland.
    There are several pathologies associated with the peritoneum, such as mesothelioma and peritonitis. Moreover, the peritoneum is widely used in ultrafiltration procedures, i.e. Read More

    Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts.
    Cells Tissues Organs 2017 4;204(3-4):125-136. Epub 2017 Oct 4.
    Division of Transplant Surgery, Department of Surgery, Mayo Clinic, Rochester, MN, USA.
    Liver transplantation from deceased or living human donors remains the only proven option for patients with end-stage liver disease. However, the shortage of donor organs is a significant clinical concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. Read More

    Protective Effect of Ginger (Zingiber officinale Roscoe) Extract against Oxidative Stress and Mitochondrial Apoptosis Induced by Interleukin-1β in Cultured Chondrocytes.
    Cells Tissues Organs 2017 7;204(5-6):241-250. Epub 2017 Sep 7.
    RAZI Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
    Aims: The protective effects of ginger (Zingiber officinale Roscoe) extract on IL-1β-mediated oxidative stress and mitochondrial apoptosis were investigated in C28I2 human chondrocytes.

    Methods: The effects of various concentrations of ginger extract on C28I2 human chondrocyte viability were evaluated in order to obtain noncytotoxic concentrations of the drug by methylthiotetrazole assay. The cells were pretreated with 5 and 25 μg/mL ginger extract for 24 h, followed by incubation with IL-1β (10 ng/mL) for 24 h. Read More

    An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration.
    Cells Tissues Organs 2017 25;204(3-4):179-190. Epub 2017 Aug 25.
    Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
    Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. Read More

    Comparison of Different Sources of Mesenchymal Stem Cells: Palatal versus Lipoaspirated Adipose Tissue.
    Cells Tissues Organs 2017 26;204(5-6):228-240. Epub 2017 Aug 26.
    Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkey.
    Objectives: The purpose of this study was to compare the proliferation and differentiation potential of mesenchymal stem cells (MSCs) derived from palatal adipose tissue (PAT) and lipoaspirated adipose tissue (LAT).

    Materials And Methods: PATs were obtained from 2 healthy female patients undergoing surgery for gingival recession, and LATs were obtained from 2 healthy female patients undergoing plastic surgery. LAT- and PAT-derived MSCs were confirmed by flow cytometry using MSC-specific surface markers. Read More

    Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding.
    Cells Tissues Organs 2017 12;204(3-4):150-163. Epub 2017 Aug 12.
    3B's Research Group (Biomaterials, Biodegradables, and Biomimetics), University of Minho, Barco, Guimarães, Portugal.
    The treatment and regeneration of bone defects caused by traumatism or diseases have not been completely addressed by current therapies. Lately, advanced tools and technologies have been successfully developed for bone tissue regeneration. Functional scaffolding materials such as biopolymers and bioresorbable fillers have gained particular attention, owing to their ability to promote cell adhesion, proliferation, and extracellular matrix production, which promote new bone growth. Read More

    Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae.
    Cells Tissues Organs 2017 12;204(3-4):137-149. Epub 2017 Aug 12.
    Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
    It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. Read More

    Neuronal Differentiation Modulated by Polymeric Membrane Properties.
    Cells Tissues Organs 2017 5;204(3-4):164-178. Epub 2017 Aug 5.
    Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, Rende, Italy.
    In this study, different collagen-blend membranes were successfully constructed by blending collagen with chitosan (CHT) or poly(lactic-co-glycolic acid) (PLGA) to enhance their properties and thus create new biofunctional materials with great potential use for neuronal tissue engineering and regeneration. Collagen blending strongly affected membrane properties in the following ways: (i) it improved the surface hydrophilicity of both pure CHT and PLGA membranes, (ii) it reduced the stiffness of CHT membranes, but (iii) it did not modify the good mechanical properties of PLGA membranes. Then, we investigated the effect of the different collagen concentrations on the neuronal behavior of the membranes developed. Read More

    Tissue Engineering of Vein Valves Based on Decellularized Natural Matrices.
    Cells Tissues Organs 2017 5;204(3-4):199-209. Epub 2017 Aug 5.
    Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), and Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Hannover, Germany.
    Valvular repair or transplantation, designed to restore the venous valve function of the legs, has been proposed as treatment in chronic venous insufficiency. Available grafts or surgeries have provided limited durability so far. Generating venous valve substitutes by means of tissue engineering could be a solution. Read More

    Vascularized Cardiac Spheroids as Novel 3D in vitro Models to Study Cardiac Fibrosis.
    Cells Tissues Organs 2017 4;204(3-4):191-198. Epub 2017 Aug 4.
    Sydney Medical School, The University of Sydney, Sydney, NSW, Australia.
    Spheroid cultures are among the most explored cellular biomaterials used in cardiovascular research, due to their improved integration of biochemical and physiological features of the heart in a defined architectural three-dimensional microenvironment when compared to monolayer cultures. To further explore the potential use of spheroid cultures for research, we engineered a novel in vitro model of the heart with vascularized cardiac spheroids (VCSs), by coculturing cardiac myocytes, endothelial cells, and fibroblasts isolated from dissociated rat neonatal hearts (aged 1-3 days) in hanging drop cultures. To evaluate the validity of VCSs in recapitulating pathophysiological processes typical of the in vivo heart, such as cardiac fibrosis, we then treated VCSs with transforming growth factor beta 1 (TGFβ1), a known profibrotic agent. Read More

    Cocultivation of Mesenchymal Stem Cells and Endothelial Progenitor Cells Reveals Antiapoptotic and Proangiogenic Effects.
    Cells Tissues Organs 2017 27;204(5-6):218-227. Epub 2017 Jul 27.
    Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany.
    Integrating bioartificial tissues into the host vasculature is a prerequisite for tissue engineering applications. Endothelial progenitor cells (EPCs) display a high angiogenic potential and a low donor-site morbidity, making them ideal for tissue engineering applications. In our study we used a murine EPC cell line (T17b) and rat mesenchymal stem cells (MSCs) for cocultivation experiments. Read More

    The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors.
    Cells Tissues Organs 2017 13;204(2):105-118. Epub 2017 Jul 13.
    Department of Experimental Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
    The olfactory bulb (OB) is a unique structure in the central nervous system that retains the ability to create new neuronal connections. Glial cells isolated from the OB have been recently considered as a novel and promising tool to establish an effective therapy for central nervous system injuries. Due to the hindered access to autologous tissue for cell isolation, an allogeneic source of tissues obtained postmortem has been proposed. Read More

    TRPC1, TRPC3, and TRPC4 in Rat Orofacial Structures.
    Cells Tissues Organs 2017 12;204(5-6):293-303. Epub 2017 Jul 12.
    Division of Dento-Oral Anesthesiology, Graduate School of Dentistry, Tohoku University, Sendai, Japan.
    TRPC (transient receptor potential cation channel subfamily C) members are nonselective monovalent cation channels and control Ca2+ inflow. In this study, immunohistochemistry for TRPC1, TRPC3, and TRPC4 was performed on rat oral and craniofacial structures to elucidate their distribution and function in the peripheries. In the trigeminal ganglion (TG), 56. Read More

    Role of Mesenchymal Stem Cells in Bone Regenerative Medicine: What Is the Evidence?
    Cells Tissues Organs 2017 24;204(2):59-83. Epub 2017 Jun 24.
    Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
    Healing and regeneration of bone injuries, particularly those that are associated with large bone defects, are a complicated process. There is growing interest in the application of osteoinductive and osteogenic growth factors and mesenchymal stem cells (MSCs) in order to significantly improve bone repair and regeneration. MSCs are multipotent stromal stem cells that can be harvested from many different sources and differentiated into a variety of cell types, such as preosteogenic chondroblasts and osteoblasts. Read More

    Recovery of Vocal Fold Epithelium after Acute Phonotrauma.
    Cells Tissues Organs 2017 24;204(2):93-104. Epub 2017 Jun 24.
    Department of Otolaryngology, Vanderbilt University School of Medicine, Nashville, TN, USA.
    We investigated the timeline of tissue repair of vocal fold epithelium after acute vibration exposure using an in vivo rabbit model. Sixty-five New Zealand white breeder rabbits were randomized to 120 min of modal- or raised-intensity phonation. After the larynges were harvested at 0, 4, 8, and 24 h, and at 3 and 7 days, the vocal fold tissue was evaluated using electron microscopy and quantitative real-time polymerase chain reaction. Read More

    Constitutive Activation of Smoothened in the Renal Collecting Ducts Leads to Renal Hypoplasia, Hydronephrosis, and Hydroureter.
    Cells Tissues Organs 2017 1;204(1):38-48. Epub 2017 Jun 1.
    Department of Anatomy, Chonbuk National University Medical School and Institute for Medical Sciences, Chonbuk National University, Jeonju, Republic of Korea.
    Sonic Hedgehog (Shh) signaling plays a major role in and is essential for regulation, patterning, and proliferation during renal development. Smoothened (Smo) plays a pivot role in transducing the Shh-glioma-associated oncogene Kruppel family member. However, the cellular and molecular mechanism underlying the role of sustained Smo activation in postnatal kidney development is still not clearly understood. Read More

    Embryonic Stem Cells for Tissue Biocompatibility, Angiogenesis, and Inflammation Testing.
    Cells Tissues Organs 2017 30;204(1):1-12. Epub 2017 May 30.
    Cardiology Division, Department of Internal Medicine I, Friedrich Schiller University Jena, Jena, Germany.
    Aim: To introduce embryoid bodies derived from mouse embryonic stem (ES) cells, which differentiate blood vessel-like structures and leukocytes, as a novel in vitro model system for biocompatibility, inflammation, and angiogenesis studies.

    Methodology/results: Punched spherical discs of bioabsorbable polymers (ε-caprolactone and L-lactide in different compositions) with a diameter of 2 mm and a thickness of 0.2 mm were inoculated with embryoid bodies for cocultivation. Read More

    Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes.
    Cells Tissues Organs 2017 12;204(1):13-24. Epub 2017 May 12.
    Institute of Veterinary Physiology, Free University of Berlin, Berlin, Germany.
    To establish the influence of fetal bovine serum (FBS) and bovine serum lipids (BSL) on cell differentiation marker expression, bovine adipose-derived stem cells from subcutaneous tissue were incubated for 14 days in 4 types of differentiation media containing 10% FBS and 10 µL/mL BSL (TRT-1), no FBS and 10 µL/mL of BSL (TRT-2), 10% FBS and no BSL (TRT-3), or no supplements (TRT-4). Cells were subjected to Nile red staining, immunocytochemistry (CD73, CD90, CD105, DLK1, FabP4), and quantitative real-time PCR (CD73, CD90, CD105, FabP4). The number of cells presenting FabP4 and the percentage of mature adipocytes with large lipid droplets were increased in TRT-2, accompanied by a robust increase in FabP4 mRNA abundance and a decrease in DLK1-positive cells. Read More

    Angiotensin II/Angiotensin II Receptor Blockade Affects Osteoporosis via the AT1/AT2-Mediated cAMP-Dependent PKA Pathway.
    Cells Tissues Organs 2017 6;204(1):25-37. Epub 2017 May 6.
    Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, China.
    Animal studies have reported on the benefits of ARB on bone mass. However, the underlying mechanism for angiotensin II (AngII)/AngII receptor blockade (ARB) in regulating bone mass remains elusive. Since high levels of plasma and urine cAMP are observed in osteoporotic and hypertensive patients, we hypothesized that cAMP may be an important molecule for the downstream events of the activation of AT receptors, members of the G-protein-coupled receptor family, in regulating bone turnover. Read More

    Sensory Neurons in the Human Geniculate Ganglion.
    Cells Tissues Organs 2017 4;204(1):49-58. Epub 2017 May 4.
    Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, Japan.
    The geniculate ganglion (GG) contains visceral and somatic sensory neurons of the facial nerve. In this study, the number and cell size of sensory neurons in the human GG were investigated. The estimated number of GG neurons ranged from 1,580 to 2,561 (mean ± SD = 1,960 ± 364. Read More

    Accessory Spleen in the Greater Omentum: Embryology and Revisited Prevalence Rates.
    Cells Tissues Organs 2017 19;203(6):374-378. Epub 2017 Apr 19.
    Department of Anatomy, Ross University School of Medicine, Roseau, Commonwealth of Dominica.
    Purpose: To investigate in a large sample the prevalence rates of accessory spleens located in the greater omentum and to explain the embryological background and the vascular supply of this rare congenital disorder.

    Methods: Evaluation of the presence of accessory spleens located in the greater omentum was performed in 5 different international anatomical centers investigating a total of 1,045 body donors. Arterial and venous blood supply and the precise location of the respective vasculature within the splenic ligaments are described based on dissection of this rare condition in a male specimen. Read More

    Extracellular Matrix Mediates BMP-2 in a Model of Temporomandibular Joint Osteoarthritis.
    Cells Tissues Organs 2017 19;204(2):84-92. Epub 2017 Apr 19.
    NIDCR, National Institutes of Health, Bethesda, MD, USA.
    Temporomandibular joint (TMJ) osteoarthritis (OA) is a complex disease that affects both cartilage and subchondral bone. It is accompanied by loss of extracellular matrix (ECM) and may be controlled by bone morphogenetic protein-2 (BMP-2). We analyzed the effect of BMP-2 in both cartilage and subchondral bone in a TMJ-OA animal model that is deficient in biglycan (Bgn) and fibromodulin (Fmod) (Bgn-/-Fmod-/-). Read More

    Enhancement of Healing of Long Tubular Bone Defects in Rabbits Using a Mixture of Atelocollagen Gel and Bone Marrow Aspirate Concentrate.
    Cells Tissues Organs 2017 17;203(6):339-352. Epub 2017 Mar 17.
    Department of Orthopedic Surgery, Uijeongbu St. Mary's Hospital, College of Medicine, Catholic University, Uijeongbu-si, Republic of Korea.
    We evaluated the bone-forming potential of a mixture of atelocollagen and bone marrow aspirate concentrate which was transplanted into bone defects. Radial shaft defects of about 10 mm in size were created in 30 New Zealand white rabbits. Ten rabbits in the control group were not treated further, 10 rabbits in the first experimental group (E1) received an atelocollagen injection, and 10 rabbits in the second experimental group (E2) received an injection of a mixture of atelocollagen and bone marrow aspirate concentrate. Read More

    Application of a Parallelizable Perfusion Bioreactor for Physiologic 3D Cell Culture.
    Cells Tissues Organs 2017 15;203(5):316-326. Epub 2017 Mar 15.
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
    It is crucial but challenging to keep physiologic conditions during the cultivation of 3D cell scaffold constructs for the optimization of 3D cell culture processes. Therefore, we demonstrate the benefits of a recently developed miniaturized perfusion bioreactor together with a specialized incubator system that allows for the cultivation of multiple samples while screening different conditions. Hence, a decellularized bone matrix was tested towards its suitability for 3D osteogenic differentiation under flow perfusion conditions. Read More

    Vascular Pathology of Ischemia/Reperfusion Injury of Rat Small Intestine.
    Cells Tissues Organs 2017 9;203(6):353-364. Epub 2017 Mar 9.
    Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
    Ischemia/reperfusion (I/R) injury of the small intestine caused by occlusion of the superior mesenteric artery affects the intestinal tissue as well as components of the blood circulatory system from the microvasculature to mesenteric vessels. The aim of this work was to study the correlation between the dynamics of destruction development in the intestinal tissue, microvasculature, and mesenteric vessels in I/R of the small intestine. The microvasculature was analyzed by whole-organ continuous monitoring of the intestinal mucosal blood perfusion by laser Doppler flowmetry during the entire I/R. Read More

    Impact of Diet and/or Exercise Intervention on Infrapatellar Fat Pad Morphology: Secondary Analysis from the Intensive Diet and Exercise for Arthritis (IDEA) Trial.
    Cells Tissues Organs 2017 22;203(4):258-266. Epub 2017 Feb 22.
    Institute of Anatomy, Paracelsus Medical University Salzburg and Nuremberg, Salzburg, Austria.
    Objectives: The infrapatellar fat pad (IPFP) represents intra-articular adipose tissue that may contribute to intra-articular inflammation and pain by secretion of proinflammatory cytokines. Here we examined the impact of weight loss by diet and/or exercise interventions on the IPFP volume.

    Methods: Intensive Diet and Exercise for Arthritis (IDEA) was a single-blinded, single-center, 18-month, prospective, randomized controlled trial that enrolled 454 overweight and obese older adults with knee pain and radiographic osteoarthritis. Read More

    Therapeutic Targeting of Epithelial Plasticity Programs: Focus on the Epithelial-Mesenchymal Transition.
    Cells Tissues Organs 2017 20;203(2):114-127. Epub 2017 Feb 20.
    Department of Radiation Oncology and Molecular Radiation Sciences, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
    Mounting data points to epithelial plasticity programs such as the epithelial-mesenchymal transition (EMT) as clinically relevant therapeutic targets for the treatment of malignant tumors. In addition to the widely realized role of EMT in increasing cancer cell invasiveness during cancer metastasis, the EMT has also been implicated in allowing cancer cells to avoid tumor suppressor pathways during early tumorigenesis. In addition, data linking EMT to innate and acquired treatment resistance further points towards the desire to develop pharmacological therapies to target epithelial plasticity in cancer. Read More

    Development of Cancer Vaccines Targeting Brachyury, a Transcription Factor Associated with Tumor Epithelial-Mesenchymal Transition.
    Cells Tissues Organs 2017 20;203(2):128-138. Epub 2017 Feb 20.
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
    Epithelial-mesenchymal transition (EMT) is recognized as a relevant process during the progression of carcinomas towards metastatic disease. Epithelial cancer cells undergoing an EMT program may acquire mesenchymal features, motility, invasiveness, and resistance to a variety of anticancer therapeutics. Preventing or reverting the EMT process in carcinomas has the potential to minimize tumor dissemination and the emergence of therapeutic resistance. Read More

    The Role of Netrins and Their Receptors in Epithelial Mesenchymal Plasticity during Development.
    Cells Tissues Organs 2017 20;203(2):71-81. Epub 2017 Feb 20.
    School of Biosciences, University of Melbourne, Melbourne, VIC, Australia.
    Transitions between mesenchymal and epithelial cells are underpinned by changes in motility, adhesion, and polarity. Netrins and their receptors can control each of these cellular properties, and are emerging as important regulators of epithelial mesenchymal plasticity (EMP). Netrins were first identified in the worm Caenorhabditis elegans as secreted chemoattractants/repellents that could guide migrating mesodermal cells and axonal growth cones. Read More

    The Role of Epithelial-Mesenchymal Transition in Chronic Obstructive Pulmonary Disease.
    Cells Tissues Organs 2017 20;203(2):99-104. Epub 2017 Feb 20.
    NHMRC Centre of Research Excellence for Chronic Respiratory Disease and Lung Aging, School of Medicine, University of Tasmania, Hobart, TAS, Australia.
    Chronic obstructive pulmonary disease (COPD) is a major cause of mortality worldwide, and there is currently no treatment that can halt the progression of the disease. Over the last decade there has been increasing interest in the idea that epithelial-mesenchymal transition (EMT) may be active in COPD. Here we review the evidence for EMT in COPD as well as the current progress being made on understanding the drivers and mechanisms involved. Read More

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