1,418 results match your criteria Cells tissues organs[Journal]


Control of Hyperglycemia Using Differentiated and Undifferentiated Mesenchymal Stem Cells in Rats with Type 1 Diabetes.

Cells Tissues Organs 2020 Jul 7:1-13. Epub 2020 Jul 7.

Razi Drug Research Center and Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran,

Due to their ability in self-renewing and differentiation into a wide variety of tissues, mesenchymal stem cells (MSCs) exhibit outstanding potential for regenerative medicine. This study was aimed at investigating different aspects of MSC therapy in controlling hyperglycemia in streptozotocin-induced diabetes rats. Using an islet cell differentiation protocol, bone marrow (BM) MSCs were differentiated into insulin-producing cells (IPCs). Read More

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http://dx.doi.org/10.1159/000507790DOI Listing
July 2020
2.137 Impact Factor

Induced Pluripotent Stem Cells for the Treatment of Liver Diseases: Novel Concepts.

Cells Tissues Organs 2020 Jul 2:1-17. Epub 2020 Jul 2.

William J. von Liebig Center for Transplantation and Clinical Regeneration, Mayo Clinic, Rochester, Minnesota, USA.

Millions of people worldwide with incurable liver disease die because of inadequate treatment options and limited availability of donor organs for liver transplantation. Regenerative medicine as an innovative approach to repairing and replacing cells, tissues, and organs is undergoing a major revolution due to the unprecedented need for organs for patients around the world. Induced pluripotent stem cells (iPSCs) have been widely studied in the field of liver regeneration and are considered to be the most promising candidate therapies. Read More

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http://dx.doi.org/10.1159/000508182DOI Listing

Lipoaspirate Storage Time and Temperature: Effects on Stromal Vascular Fraction Quality and Cell Composition.

Cells Tissues Organs 2020 Jun 24:1-10. Epub 2020 Jun 24.

Department of Clinical Immunology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

The adipose tissue-derived stromal vascular fraction (SVF) is a promising candidate for use in cell therapy and tissue engineering due to its regenerative and immunomodulatory properties. Some therapies are based on using the complete SVF product, whereas others depend on the expansion of adipose-derived stromal cells (ASCs) in culture. The latter application often involves a time delay between adipose tissue harvest and SVF isolation. Read More

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http://dx.doi.org/10.1159/000507825DOI Listing

White Tea Reduced Bone Loss by Suppressing the TRAP/CTX Pathway in Ovariectomy-Induced Osteoporosis Model Rats.

Cells Tissues Organs 2020 Jun 19:1-10. Epub 2020 Jun 19.

Department of Radiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.

Osteoporosis is an important skeletal disease characterized by bone weakness and high risk of fracture in postmenopausal women. Tea consumption is known to play an important role in the prevention or alleviation of osteoporosis. However, the therapeutic effects of aqueous extracts of white tea (WT) have not been evaluated in osteoporosis rat models. Read More

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http://dx.doi.org/10.1159/000507791DOI Listing

Decellularized Porcine Epiphyseal Plate-Derived Extracellular Matrix Powder: Synthesis and Characterization.

Cells Tissues Organs 2020 Jun 15:1-9. Epub 2020 Jun 15.

Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea,

The aim of this study was to develop a porcine epiphyseal plate-derived extracellular matrix powder (PEPEP) for epiphyseal plate regeneration. PEPEP was characterized by chemical assay to determine the contents of DNA and epiphyseal plate complex chemical components (glycosaminoglycan and hydroxyproline). The effects of PEPEP on the viability, proliferation, and differentiation of human bone marrow mesenchymal stem cells (hBMSCs) were also evaluated. Read More

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http://dx.doi.org/10.1159/000507552DOI Listing

A Biobank of Stem Cells of Human Exfoliated Deciduous Teeth: Overview of Applications and Developments in Brazil.

Cells Tissues Organs 2020 Jun 15:1-6. Epub 2020 Jun 15.

Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.

A biobank is an organized collection of biological human material and its associated information stored for research according to regulations under institutional responsibility, without commercial purposes, being a mandatory and strategical activity for research, regenerative medicine, and innovation. Stem cells have largely been employed in research and frequently stored in biobanks, which have been used as an essential source of biological materials. Stem cells of human exfoliated deciduous teeth (SHED) are stem cells which have a high multipotency and can be easily obtained. Read More

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http://dx.doi.org/10.1159/000506677DOI Listing

Impact of Aging on the Characterization of Brown and White Adipose Tissue-Derived Stem Cells in Mice.

Cells Tissues Organs 2020 Jun 11:1-11. Epub 2020 Jun 11.

School of Life Sciences, Guangxi Normal University, Guilin, China,

Adipose tissue enriched with adipose tissue-derived stem cells (ASCs) is often used for stem cell-based therapies. However, the characteristics of ASCs from different types of adipose tissue have varying biochemical and functional properties. We aimed to investigate how age affected the biological and functional characteristics of ASCs from brown (BAT) and white adipose tissue (WAT). Read More

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http://dx.doi.org/10.1159/000507434DOI Listing
June 2020
2.137 Impact Factor

Lectin Profile Variation in Mesenchymal Stem Cells Derived from Different Sources.

Cells Tissues Organs 2019 28;208(3-4):101-112. Epub 2020 May 28.

Laboratory for Stem Cell Research, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

Human mesenchymal stem cells (MSCs), a promising source of stem cells for regenerative medicine, have different morphological and functional characteristics. Carbohydrate moieties on the cell surface play an important role, including cell-cell interaction and cell recognition. The objective of this study was to determine possible differences in glycoconjugate distribution patterns of MSCs derived from various sources. Read More

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http://dx.doi.org/10.1159/000505238DOI Listing

Gene Expression Profiling of Human Adipose Tissue Stem Cells during 2D versus 3D Adipogenesis.

Cells Tissues Organs 2019 28;208(3-4):113-133. Epub 2020 May 28.

Department of Biomedical, Industrial, and Human Factors Engineering, Wright State University, Dayton, Ohio, USA,

Much of the current understanding on molecular and cellular events of adipose developmental biology comes from monolayer cell culture models using preadipocyte cell lines, although in vivo adipose tissue consists of a much more complex three-dimensional microenvironment of diverse cell types, extracellular network, and tissue-specific morphological and functional features. Added to this fact, the preadipocytes, on which the adipogenesis mechanisms are mostly explored, possess some serious limitations (e.g. Read More

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http://dx.doi.org/10.1159/000507187DOI Listing

Neuropathic Pain Causes a Decrease in the Dendritic Tree Complexity of Hippocampal CA3 Pyramidal Neurons.

Cells Tissues Organs 2019 27;208(3-4):89-100. Epub 2020 May 27.

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russian Federation.

The International Pain Association defines neuropathic pain as "an unpleasant sensory and emotional experience associated with actual or potential tissue damage." Recent studies show that chronic neuropathic pain causes both morphological and functional changes within brain structures. Due to the impact of supraspinal centers on pain signal processing, patients with chronic pain often suffer from depression, anxiety, memory impairment, and learning disabilities. Read More

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http://dx.doi.org/10.1159/000506812DOI Listing

Understanding the Role of the Clitellum in the Regeneration Events of the Earthworm Eudrilus eugeniae.

Cells Tissues Organs 2019 16;208(3-4):134-141. Epub 2020 May 16.

Regeneration and Stem Cell Biology Laboratory, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India.

Regeneration is a complex mechanism to restore lost or damaged body parts. In earthworms, regeneration capability varies among different species, and it is important to explore the mechanism behind the regeneration process. Interestingly, regeneration in earthworms is either dependent or independent of clitellum segments. Read More

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http://dx.doi.org/10.1159/000507243DOI Listing

Cinnamaldehyde Enhanced Functional Recovery after Sciatic Nerve Crush Injury in Rats.

Cells Tissues Organs 2020 May 11:1-11. Epub 2020 May 11.

Neuroscience Research Center, Guilan University of Medical Sciences, Rasht, Iran,

Peripheral nerve injury is a common clinical issue induced by trauma, tumor, and damage caused by treatment. Such factors create chemical and inflammatory alterations at the injury site, which increase nerve deterioration. Thus, minimizing these modifications can lead to nerve protection after injury. Read More

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http://dx.doi.org/10.1159/000507016DOI Listing

Alleviation of Simvastatin-Induced Myopathy in Rats by the Standardized Extract of Ginkgo Biloba (EGb761): Insights into the Mechanisms of Action.

Cells Tissues Organs 2019 5;208(3-4):158-176. Epub 2020 May 5.

Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt,

Statins are the most widely prescribed cholesterol-lowering drugs to reduce the risk of cardiovascular diseases. Statin-induced myopathy is the major side effect of this class of drugs. Here, we studied whether standardized leaf extracts of ginkgo biloba (EGb761) would improve simvastatin (SIM)-induced muscle changes. Read More

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http://dx.doi.org/10.1159/000507048DOI Listing

Comparative Proteome Analyses of Ureteropelvic Junction Obstruction and Surrounding Ureteral Tissue.

Cells Tissues Organs 2020 Apr 7:1-11. Epub 2020 Apr 7.

Department of Pediatric Surgery, Faculty of Medicine, Istanbul Bilim University, Istanbul, Turkey.

Ureteropelvic junction (UPJ) obstruction is a common problem in children, but its etiology remains unclear. In this study, the proteome profiles of the obstructed segment and its surrounding distal and proximal parts were comparatively evaluated. Twelve children younger than 2 years of age with unilateral intrinsic UPJ obstruction were included. Read More

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http://dx.doi.org/10.1159/000506736DOI Listing

HT-29 and Caco2 Cell Lines Are Suitable Models for Studying the Role of Arachidonic Acid-Metabolizing Enzymes in Intestinal Cell Differentiation.

Cells Tissues Organs 2019 3;208(1-2):37-47. Epub 2020 Apr 3.

Department of Clinical and Molecular Pathology and Laboratory of Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czechia.

Introduction: Cytochrome (CYP) epoxygenases (CYP2C and CYP2J) and soluble epoxide hydrolase (sEH) participate in the metabolism of arachidonic acid and may also have a potential role in enterocyte differentiation. The first critical step in the study of intestinal cell differentiation is the determination of a suitable in vitro model, which must be as similar as possible to the conditions of a living organism. It is known that HT-29 and Caco2 cell lines derived from human colorectal carcinomas can differentiate into enterocyte-like cells in appropriate culture conditions. Read More

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http://dx.doi.org/10.1159/000506735DOI Listing

Improvement of in situ Follicular Activation and Early Development in Cryopreserved Human Ovarian Cortical Tissue by Co-Culturing with Mesenchymal Stem Cells.

Cells Tissues Organs 2019;208(1-2):48-58. Epub 2020 Mar 23.

Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran,

Follicular loss and tissue degeneration are great challenges in ovarian tissue culture systems. Mesenchymal stem cells (MSC) secrete a cocktail of growth factors and cytokines which supports adjacent cells and tissues. The aim of the current study was to investigate the impact of human bone marrow (hBM)-MSC, as co-culture cells, on human follicular development in ovarian cortical tissue (OCT) culture. Read More

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http://dx.doi.org/10.1159/000506303DOI Listing
March 2020
2.137 Impact Factor

High-Phosphate Diet Improved the Skeletal Development of Fam20c-Deficient Mice.

Cells Tissues Organs 2019 26;208(1-2):25-36. Epub 2020 Feb 26.

Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, Texas, USA.

FAM20C (family with sequence similarity 20 - member C) is a protein kinase that phosphorylates secretory proteins, including the proteins that are essential to the formation and mineralization of calcified tissues. Previously, we reported that inactivation of Fam20c in mice led to hypophosphatemic rickets/osteomalacia along with increased circulating fibroblast growth factor 23 (FGF23) levels and dental defects. In this study, we examined whether a high-phosphate (hPi) diet could rescue the skeletal defects in Fam20c-deficient mice. Read More

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http://dx.doi.org/10.1159/000506005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192785PMC
February 2020

Effect of MicroRNA-20a on Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells.

Cells Tissues Organs 2019 25;208(3-4):148-157. Epub 2020 Feb 25.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Osteogenic differentiation of human adipose tissue-derived stem cells (hASCs) is a complex process that is regulated by multiple factors, including microRNAs (miRNAs). The miRNA miR-20a was shown to promote bone formation from bone marrow-derived mesenchymal stem cells. However, the role of miR-20a in osteogenic differentiation of hASCs remains unclear. Read More

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http://dx.doi.org/10.1159/000506304DOI Listing
February 2020
2.137 Impact Factor

Investigation of the Effect of Secreted Factors from Mesenchymal Stem Cells on Disc Cells from Degenerated Discs.

Cells Tissues Organs 2019;208(1-2):76-88. Epub 2020 Feb 24.

Department of Orthopedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Low back pain is experienced by a large number of people in western countries and may be caused and influenced by many different pathologies and psychosocial factors including disc degeneration. Disc degeneration involves the increased expression of proinflammatory cytokines and matrix metalloproteinases (MMPs) in the disc environment, which leads to the loss of extracellular matrix (ECM) and the viability of the native disc cells (DCs). Treatment approaches using growth factors and cell therapy have been proposed due to the compelling results that growth factors and mesenchymal stem cells (MSCs) can influence the degenerated discs. Read More

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http://dx.doi.org/10.1159/000506350DOI Listing
February 2020

Effects of Structure Characteristics of Osteocyte Lacunae on Squeeze Damage Resistance of Osteons.

Cells Tissues Organs 2019 18;208(3-4):142-147. Epub 2020 Feb 18.

College of Aerospace Engineering, Chongqing University, Chongqing, China.

Scanning electron-microscopic observations show that there are many osteocyte lacunae between the circumferential lamellae, which are nonuniform in distribution. The lacunae are elliptical, and their major axes are along the circumferential direction of the osteon, which can be denoted as circumferential elliptical lacunae (CE). The observations also show that there are many circumferential microcracks in the osteons, and these microcracks are initiated from 2 endpoints of the major CE axis. Read More

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http://dx.doi.org/10.1159/000505135DOI Listing
February 2020

MiR-375 Mediates Chondrocyte Metabolism and Oxidative Stress in Osteoarthritis Mouse Models through the JAK2/STAT3 Signaling Pathway.

Cells Tissues Organs 2019;208(1-2):13-24. Epub 2020 Feb 11.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China,

Objective: The aim of this work was to determine the effect of miR-375 on chondrocyte metabolism and oxidative stress in osteoarthritis (OA) mouse models through the JAK2/STAT3 signaling pathway.

Methods: Chondrocytes were divided into control, IL-1β, IL-1β + miR-375 mimic, IL-1β + miR-375 inhibitor, IL-1β + miR-NC (negative control), and IL-1β + miR-375 inhibitor + siJAK2 groups. The chondrocyte proliferation was determined by MTT assay, the superoxide dismutase (SOD) and malondialdehyde (MDA) levels by corresponding kits, and the chondrocyte apoptosis by TUNEL staining. Read More

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http://dx.doi.org/10.1159/000504959DOI Listing
February 2020
2.137 Impact Factor

Investigation of Cellular Function and DNA Integrity during 2D in vitro Culture of Human Salivary Gland Epithelial Cells.

Cells Tissues Organs 2019;208(1-2):66-75. Epub 2020 Feb 5.

Department of Otorhinolaryngology, Plastic, Aesthetic, and Reconstructive Head and Neck Surgery, University Hospital Würzburg, Würzburg, Germany.

In vitro culture of human salivary gland epithelial cells (SGEC) is still a challenge. A high quantity and quality of cells are needed for the cultivation of 3D matrices. Furthermore, it is known that DNA damage is supposed to be an important factor involved in carcinogenesis. Read More

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http://dx.doi.org/10.1159/000505433DOI Listing
February 2020

Withaferin-A Protects the Nigral Dopamine Neuron and Recovers Motor Activity in Aged Rats.

Cells Tissues Organs 2019;208(1-2):59-65. Epub 2020 Jan 24.

Velammal Medical College and Hospital, Madurai, India,

Withaferin-A (WA) was evaluated for its neuroprotective efficacy on the dopamine (DA) neurons of the substantia nigra (SN) and striatum (ST) in aged rats. Wistar albino rats were divided into group I, young (3 months old); group II, aged (24 months old); group III, aged rats supplemented with WA (50 mg/kg bodyweight once per day for 30 days), and group IV, young rats supplemented with WA (50 mg/kg bodyweight). At the end of the experiment period, the animals were subjected to various motor behavior analyses, and were sacrificed by transcardial perfusion. Read More

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http://dx.doi.org/10.1159/000505183DOI Listing
January 2020

Muscle Spindle Density of Lateral Rotators of the Thigh in Japanese Macaques and a Gibbon.

Cells Tissues Organs 2019 10;208(1-2):1-12. Epub 2020 Jan 10.

Department of Biological Anthropology, Graduate School of Human Sciences, Osaka University, Suita, Japan.

We examined the six small lateral rotators of the hip joint, which is one of the most flexible joints and allows kinematically complex motions of the hindlimb, to elucidate the functional differentiation among these muscles and to test the hypothesis that species-specific characteristics in hindlimb use during locomotion are reflected in the muscle spindle density and in other parameters of the deep small hip joint rotators. For these purposes, we estimated the number of muscle spindles of the superior gemellus muscle (SG), inferior gemellus muscle, quadratus femoris muscle, obturator internus muscle (OI), obturator externus muscle, and piriformis muscle in three Japanese macaques and a gibbon, using 30-µm-thick serial sections throughout each muscle length after azan staining. The numbers of muscle spindles per 10,000 muscle fibers were determined to compare inter-muscle variation. Read More

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http://dx.doi.org/10.1159/000504958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212700PMC
January 2020

Dynamic Changes in the Localization of Neuronatin-Positive Cells during Neurogenesis in the Embryonic Rat Brain.

Cells Tissues Organs 2019 5;207(3-4):127-137. Epub 2019 Dec 5.

Division of Life Sciences, Graduate School of Agriculture, Meiji University, Kawasaki, Japan,

Neuronatin (NNAT) was first identified as a gene selectively and abundantly expressed in the cytoplasm of the newborn mouse brain, and involved in neonatal neurogenesis. However, the particular roles of NNAT in the developing prenatal brain have not been identified, especially in mid to late stages. In this study, we performed immunohistochemical analyses of NNAT and SOX2 proteins, a nuclear transcription factor and neural stem/progenitor marker, in the rat brain on embryonic days 13. Read More

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http://dx.doi.org/10.1159/000504359DOI Listing

Prenatal Effects of a 1,800-MHz Electromagnetic Field on Rat Livers.

Cells Tissues Organs 2019 4;207(3-4):187-196. Epub 2019 Dec 4.

Department of Medical Services and Techniques, Health Care Services Vocational School, Recep Tayyip Erdogan University, Rize, Turkey.

The use of devices, including mobile phones, generating electromagnetic fields (EMF) is widespread and is progressively increasing. It has also been shown that EMF may have detrimental effects. This is the first study to investigate the postnatal biochemical and histological effects of prenatal exposure of rat livers to 1,800-MHz EMF at different time intervals in uteroplacental life. Read More

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http://dx.doi.org/10.1159/000504506DOI Listing

Preparation and Comparison of Effects of Different Herbal Oil Ointments as Wound-Healing Agents.

Cells Tissues Organs 2019 27;207(3-4):177-186. Epub 2019 Nov 27.

Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran,

Wound healing is a complex process and some agents have been reported to accelerate it. The aim of this study was to evaluate the healing effect of Eucerin-based ointments of lemon, sesame and olive oils on infected full-thickness wounds in rats. Wounds were created on the dorsal surface of Male Albino Wistar rats (n = 12). Read More

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http://dx.doi.org/10.1159/000503624DOI Listing

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects.

Cells Tissues Organs 2019 15;207(3-4):138-148. Epub 2019 Nov 15.

Departamento de Ciências Morfológicas, Universidade Federal de Santa Catarina, Florianópolis, Brazil,

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Read More

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http://dx.doi.org/10.1159/000502513DOI Listing

Overexpression of miR-150 Inhibits the NF-κB Signal Pathway in Intervertebral Disc Degeneration through Targeting P2X7.

Cells Tissues Organs 2019 14;207(3-4):165-176. Epub 2019 Nov 14.

Department of Orthopedics, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou, China,

Objective: To elaborate the mechanism of miR-150 in the regulation of the NF-κB signal pathway in intervertebral disc degeneration (IDD) by targeting P2X7.

Methods: The degenerative and normal intervertebral disc tissues were collected to detect the expressions of miR-150 and P2X7. Nucleus pulposus cells were transfected and divided into different groups. Read More

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http://dx.doi.org/10.1159/000503281DOI Listing

In Search of Imprints Left by the Impairment of Nephrogenesis.

Authors:
Will W Minuth

Cells Tissues Organs 2019 31;207(2):69-82. Epub 2019 Oct 31.

Institute of Anatomy, University of Regensburg, Regensburg, Germany,

Clinical aspects dealing with the impairment of nephrogenesis in preterm and low birth weight babies were intensely researched. In this context it was shown that quite different noxae can harm nephron formation, and that the morphological damage in the fetal kidney is rather complex. Some pathological findings show that the impairment leads to changes in developing glomeruli that are restricted to the maturation zone of the outer cortex in the fetal human kidney. Read More

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http://dx.doi.org/10.1159/000504085DOI Listing

Aged Osteoporotic Bone Marrow Stromal Cells Demonstrate Defective Recruitment, Mechanosensitivity, and Matrix Deposition.

Cells Tissues Organs 2019 25;207(2):83-96. Epub 2019 Oct 25.

Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College, Dublin, Ireland,

Bone formation requires the replenishment of the osteoblast from a progenitor or stem cell population, which must be recruited, expanded, and differentiated to ensure continued anabolism. How this occurs and whether it is altered in the osteoporotic environment is poorly understood. Furthermore, given that emerging treatments for osteoporosis are targeting this progenitor population, it is critical to determine the regenerative capacity of this cell type in the setting of osteoporosis. Read More

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http://dx.doi.org/10.1159/000503444DOI Listing

Investigating the Osteoinductive Potential of a Decellularized Xenograft Bone Substitute.

Cells Tissues Organs 2019 25;207(2):97-113. Epub 2019 Oct 25.

Orthopaedic Surgery, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA,

Bone grafting is the second most common tissue transplantation procedure worldwide. One of the alternative methods for bone repair under investigation is a tissue-engineered bone substitute. An ideal property of tissue-engineered bone substitutes is osteoinductivity, defined as the ability to stimulate primitive cells to differentiate into a bone-forming lineage. Read More

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http://dx.doi.org/10.1159/000503280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935535PMC

Human Platelet Lysate Sustains the Osteogenic/Adipogenic Differentiation Potential of Adipose-Derived Mesenchymal Stromal Cells and Maintains Their DNA Integrity in vitro.

Cells Tissues Organs 2019 8;207(3-4):149-164. Epub 2019 Oct 8.

National Medical Research Centre of Radiology, Moscow, Russian Federation.

Human platelet lysate (HPL) is a promising alternative to fetal calf serum (FCS) for the expansion of adipose tissue mesenchymal stromal cells (AT-MSCs) for translational medicine applications. However, some biological effects of HPL are still to be elucidated. We aimed to compare complex characteristics, such as cell morphology, proliferative activity, differentiation potential, and especially monolayer recovery, DNA integrity, and the gene expression pattern, between AT-MSCs cultured with HPL or FCS. Read More

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http://dx.doi.org/10.1159/000502813DOI Listing

DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation.

Cells Tissues Organs 2019 1;207(3-4):115-126. Epub 2019 Oct 1.

Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Stem cells have essential applications in in vitro tissue engineering or regenerative medicine. However, there is still a need to understand more deeply the mechanisms of stem cell differentiation and to optimize the methods to control stem cell function. In this study, we first investigated the activity of DNA methyltransferases (DNMTs) during chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) and found that DNMT3A and DNMT3B were markedly upregulated during hBMSC chondrogenic differentiation. Read More

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http://dx.doi.org/10.1159/000502885DOI Listing
July 2020
1 Read

Platelet-Rich Plasma and Bone Marrow-Derived Mesenchymal Stromal Cells Prevent TGF-β1-Induced Myofibroblast Generation but Are Not Synergistic when Combined: Morphological in vitro Analysis.

Cells Tissues Organs 2018 5;206(6):283-295. Epub 2019 Aug 5.

Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Florence, Italy,

The persistence of activated myofibroblasts is a hallmark of fibrosis of many organs. Thus, the modulation of the generation/functionality of these cells may represent a strategical anti-fibrotic therapeutic option. Bone marrow-derived mesenchymal stromal cell (MSC)-based therapy has shown promising clues, but some criticisms still limit the clinical use of these cells, including the need to avoid xenogeneic compound contamination for ex vivo cell amplification and the identification of appropriate growth factors acting as a pre-conditioning agent and/or cell delivery vehicle during transplantation, thus enabling the improvement of cell survival in the host tissue microenvironment. Read More

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http://dx.doi.org/10.1159/000501499DOI Listing

Effect of Modified Bovine Pericardium on Human Gingival Fibroblasts in vitro.

Cells Tissues Organs 2018 29;206(6):296-307. Epub 2019 Jul 29.

Laboratory of Tissue Engineering and Biomedical Materials, Department of Physiology and Animal Biotechnology, University of Science, Vietnam National University, Ho Chi Minh, Vietnam,

Supportive membranes have recently been applied to treat periodontal disease in order to achieve periodontal tissue regeneration. The crucial role of these membranes is to facilitate the restoration of the structural and functional periodontium. Bovine pericardium (BP) is mainly composed of collagen type I, which was demonstrated to have good mechanical properties and biological regenerative potential. Read More

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http://dx.doi.org/10.1159/000501807DOI Listing

A CD34+ Cell Enrichment Protocol of Hematopoietic Stem Cells in a Well-Established Quality Management System.

Cells Tissues Organs 2019 29;207(1):15-20. Epub 2019 Jul 29.

Stem Cell Institute, Ankara University, Ankara, Turkey.

Allogeneic stem cell transplantation applications have improved tremendously over the past quarter of a century. The use of new immunosuppressive protocols and elimination of T cells by CD34+ cell enrichment or T cell depletion on apheresis products increases the chance of using partially matched or haploidentical grafts. This is without increasing the risk of graft-versus-host disease, which is observed as a major complication of hematopoietic stem cell transplantation. Read More

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http://dx.doi.org/10.1159/000501167DOI Listing
March 2020
3 Reads
2.137 Impact Factor

Human Bone Marrow Mesenchymal Stem Cell Conditioned Medium Promotes Wound Healing in Deep Second-Degree Burns in Male Rats.

Cells Tissues Organs 2018 24;206(6):317-329. Epub 2019 Jul 24.

Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran,

Burn wound treatment is difficult and one of the most challenging problems in the clinic. Researchers have examined the applications of mesenchymal stem cells as a cell-based therapy for skin regeneration. But the role of human bone marrow mesenchymal stem cell conditioned medium (hBM-MSC-CM) in the treatment of burn injury remains unclear. Read More

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http://dx.doi.org/10.1159/000501651DOI Listing

Will Nanotechnology Bring New Hope for Stem Cell Therapy?

Cells Tissues Organs 2018 9;206(4-5):229-241. Epub 2019 Jul 9.

Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

The potential of stem cell therapy has been shown in preclinical trials for the treatment of damage and replacement of organs and degenerative diseases. After many years of research, its clinical application is limited. Currently there is not a single stem cell therapy product or procedure. Read More

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http://dx.doi.org/10.1159/000500517DOI Listing
July 2019
2.137 Impact Factor

Effects of Infliximab against Methotrexate Toxicity in Splenic Tissue via the Regulation of CD3, CD68, and C200R in Rats.

Cells Tissues Organs 2018 8;206(6):308-316. Epub 2019 Jul 8.

Department of Internal Medicine, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.

Methotrexate (MTX), which has been used in clinical practice for approximately 70 years, is still widely employed in the treatment of rheumatoid arthritis (RA), psoriasis, and cancer. Although MTX toxicity causes nephrotoxicity, hepatotoxicity, bone marrow suppression, pulmonary fibrosis, and gastrointestinal damage, previous studies have not addressed splenic toxicity. This is the first study to examine the effectiveness of infliximab (INF) against MTX-induced toxicity in splenic tissues via the regulation of CD3, CD68, and C200R. Read More

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http://dx.doi.org/10.1159/000500905DOI Listing

Hippocampal Neurogenesis in Conditions of Chronic Stress Induced by Sciatic Nerve Injury in the Rat.

Cells Tissues Organs 2019 8;207(1):58-68. Epub 2019 Jul 8.

National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russian Federation,

The dentate gyrus of the hippocampus is the primary location of adult neurogenesis, which is affected by a variety of external and internal factors, including activity of surrounding glial cells. This study concerns alterations in hippocampal neurogenesis and changes in activity of both proinflammatory and neuroprotective microglia/macrophages after sciatic nerve injury in the rat. Here, we demonstrated that the chronic pain induced by a peripheral nerve injury manifests in the hippocampus by a decrease in proliferation (PCNA+) and neurogenesis (DCX+), an increase in proinflammatory cytokines (CD86+), and a reduction in neuroprotective (CD163+) microglia/macrophages. Read More

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http://dx.doi.org/10.1159/000501236DOI Listing
March 2020
1 Read

Pathological Effects of Cortisol on Intervertebral Disc Cells and Mesenchymal Stem Cells from Lower Back Pain Patients.

Cells Tissues Organs 2019 3;207(1):34-45. Epub 2019 Jul 3.

Department of Orthopedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

In western countries, lower back pain (LBP) is one of the most common disorders, experienced by more than 80% of the population. Chronic LBP due to disc degeneration has been linked to ongoing inflammatory processes in the disc and endplates. Pain effects the body in different ways, inducing a general stress response in which the body responds by releasing the stress hormone cortisol. Read More

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http://dx.doi.org/10.1159/000500772DOI Listing
March 2020
2.137 Impact Factor

In vitro Engineering of a Skin Substitute Based on Adipose-Derived Stem Cells.

Cells Tissues Organs 2019 1;207(1):46-57. Epub 2019 Jul 1.

Surgical, Medical and Dental Department of Morphological Sciences Related to Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.

In the field of wound healing, stem cell-based strategies are gaining importance for their regenerative potential. Adipose-derived stem cells (ADSCs) are a particular subset of mesenchymal stem cells present in the stromal-vascular fraction of the adipose tissue, today considered very attractive for their relative abundance and accessibility in the human body. However, ADSCs are still not routinely used in normal clinical practice. Read More

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http://dx.doi.org/10.1159/000501071DOI Listing
March 2020
4 Reads

Anticatabolic Effects of Morin through the Counteraction of Interleukin-1β-Induced Inflammation in Rat Primary Chondrocytes.

Cells Tissues Organs 2019 28;207(1):21-33. Epub 2019 Jun 28.

Pre-Dentistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea,

Morin, a flavonoid isolated from various medicinal herbal plants, has an anti-inflammatory effect. This study aimed to elucidate the anticatabolic effects and cellular mechanism of morin against interleukin-1β (IL-1β) in rat primary chondrocytes. Morin at 10-100 μM did not affect the viability of rat primary chondrocytes. Read More

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http://dx.doi.org/10.1159/000500323DOI Listing
March 2020
3 Reads

Morphological and Immunohistochemical Characterization of Human Intrinsic Gastric Neurons.

Cells Tissues Organs 2018 21;206(4-5):183-195. Epub 2019 Jun 21.

Institute of Anatomy and Cell Biology, Friedrich Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany,

Our knowledge about human gastric enteric neuron types is even more limited than that of human intestinal types. Here, we immunohistochemically stained wholemounts and sections of gastric specimens obtained from 18 tumor-resected patients. Myenteric wholemounts were labeled for choline acetyl transferase (ChAT), neuronal nitric oxide synthase (NOS), and the human neuronal protein HuC/D (as pan-neuronal marker for quantitative analysis) or alternatively for neurofilament (for morphological evaluation). Read More

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http://dx.doi.org/10.1159/000500566DOI Listing
June 2019
15 Reads

Unravelling the Role of MAPKs (ERK1/2) in Venous Reflux in Patients with Chronic Venous Disorder.

Cells Tissues Organs 2018 14;206(4-5):272-282. Epub 2019 Jun 14.

Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences and Networking Biomedical Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), University of Alcalá, Alcalá de Henares, Spain.

Chronic venous disorder (CVeD), is a disorder in which there is a modification in the conditions of blood return to the heart. The disorder may arise from incompetent valves and the resultant venous reflux (chronic venous insufficiency, CVI). The economic burden of CVeD on health systems is high, and research efforts have sought to elucidate the mechanisms involved as possible therapeutic targets. Read More

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http://dx.doi.org/10.1159/000500449DOI Listing
June 2019
28 Reads

The Hinrichsen Embryology Collection: Digitization of Historical Histological Human Embryonic Slides and MRI of Whole Fetuses.

Cells Tissues Organs 2019 12;207(1):1-14. Epub 2019 Jun 12.

Department of Anatomy and Molecular Embryology, Faculty of Medicine, Ruhr University Bochum, Bochum, Germany.

The number of human embryology collections is very limited worldwide. Some of these comprise the Carnegie Collection, Kyoto Collection, and the Blechschmidt Collection. One further embryonic collection is the Hinrichsen Collection of the Ruhr University Bochum, Germany, which also contains very well-preserved embryos/fetuses, along with approximately 16,000 histological sections. Read More

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http://dx.doi.org/10.1159/000500018DOI Listing
March 2020
1 Read

Morphometric, Densitometric, and Mechanical Properties of Maxillary Teeth in 5-Month-Old Polish Merino Sheep.

Cells Tissues Organs 2018 23;206(4-5):196-207. Epub 2019 May 23.

II Department of Radiology, Medical University in Lublin, Lublin, Poland.

This study was performed to determine morphological, densitometric, mechanical, and elemental characteristics of maxillary teeth in 5-month-old Polish Merino sheep. The total tooth volume (Tvol) was determined using quantitative computed tomography. Micro-computed tomography was used to determine the total enamel volume (Evol), volumetric enamel mineral density, total dentine volume (Dvol), volumetric dentine mineral density, and total tooth enamel and dentine volume (EDvol). Read More

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http://dx.doi.org/10.1159/000499479DOI Listing
May 2019
3 Reads

Modeling the Effects of Yoga on the Progression of Alzheimer's Disease in a Dish.

Cells Tissues Organs 2018 23;206(4-5):263-271. Epub 2019 May 23.

Division of Medical Sciences, University of Victoria, Victoria, British Columbia, Canada,

Alzheimer's disease (AD) accounts for 80% of all dementia cases, making it the most common form of dementia. Aging serves as the main risk factor for AD, but early onset AD can also occur in individuals younger than 65 years. AD results from progressive neurodegeneration leading to dysfunctional synaptic transmission in the brain. Read More

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http://dx.doi.org/10.1159/000499503DOI Listing
May 2019
23 Reads

Gestational Age-Dependent Fetal Fluid Dynamics in the Ovine Developmental Model: Establishment of Surrogate Markers for the Differentiation of Stem Cell Origin.

Cells Tissues Organs 2018 10;206(4-5):208-217. Epub 2019 May 10.

Institute for Regenerative Medicine (IREM), University of Zurich, Zurich, Switzerland,

The ovine developmental model represents the standard in vivo model for studies involving maternofetal physiology, amniotic fluid (AF) research, and fetal cell therapy prior to human clinical use. Although being close to the human fetal anatomy, 2 separate extraembryonic fluid compartments remain during gestation, known as the amnion and the allantois. A clear distinction between AF versus allantoic fluid (AL) is therefore indispensable for correct scientific conclusions with regard to human translation. Read More

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http://dx.doi.org/10.1159/000499504DOI Listing
May 2019
5 Reads