1,343 results match your criteria Cell structure and function[Journal]


Prickle2 and Igsf9b coordinately regulate the cytoarchitecture of the axon initial segment.

Cell Struct Funct 2020 Jul 8. Epub 2020 Jul 8.

Institute for Comprehensive Medical Science, Fujita Health University.

Prickle2 has been identified in genetic studies of subjects with autism spectrum disorder (ASD) and epilepsy, but the pathological mechanism of Prickle2 remains to be fully understood. Proteomic analysis of Prickle2 with mass spectrometry revealed twenty-eight Prickle2 interactors, including immunoglobulin superfamily member 9b (Igsf9b), in the brain. Here, because Igsf9 family proteins are associated with psychiatric diseases and seizures, we studied the physiological interaction between Prickle2 and Igsf9b. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20028DOI Listing

p18/Lamtor1-mTORC1 Signaling Controls Development of Mucin-producing Goblet Cells in the Intestine.

Cell Struct Funct 2020 ;45(2):93-105

Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University.

Mechanistic target of rapamycin complex 1 (mTORC1) plays a pivotal role in controlling cell growth and metabolism in response to nutrients and growth factors. The activity of mTORC1 is dually regulated by amino acids and growth factor signaling, and amino acid-dependent mTORC1 activity is regulated by mTORC1 interaction with the Ragulator-Rag GTPase complex, which is localized to the surface of lysosomes via a membrane-anchored protein, p18/Lamtor1. However, the physiological function of p18-Ragulator-dependent mTORC1 signaling remains elusive. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20018DOI Listing
January 2020

Internalization of AMPA-type glutamate receptor in the MIN6 pancreatic β-cell line.

Cell Struct Funct 2020 Jun 24. Epub 2020 Jun 24.

Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.

The activity of AMPA-type glutamate receptor is involved in insulin release from pancreatic β-cells. However, the mechanism and dynamics that underlie AMPA receptor-mediated insulin release in β-cells is largely unknown. Here, we show that AMPA induces internalization of glutamate receptor 2/3 (GluR2/3), AMPA receptor subtype, in the mouse β-cell line MIN6. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20020DOI Listing

Biliverdin reductase-A deficiency brighten and sensitize biliverdin-binding chromoproteins.

Cell Struct Funct 2020 Jun 25. Epub 2020 Jun 25.

Laboratory of Bioimaging and Cell Signaling, Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University.

Tissue absorbance, light scattering, and autofluorescence are significantly lower in the near-infrared (NIR) range than in the visible range. Because of these advantages, NIR fluorescent proteins (FPs) are in high demand for in vivo imaging. Nevertheless, application of NIR FPs such as iRFP is still limited due to their dimness in mammalian cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20010DOI Listing

Visualization of procollagen IV reveals ER-to-Golgi transport by ERGIC-independent carriers.

Cell Struct Funct 2020 Jun 18. Epub 2020 Jun 18.

Laboratory of Molecular and Cellular Biology, Institute for Frontier Life and Medical Sciences, Kyoto University.

Collagen is the most abundant protein in animal tissues and is critical for their proper organization. Nascent procollagens in the endoplasmic reticulum (ER) are considered too large to be loaded into coat protein complex II (COPII) vesicles, which have a diameter of 60-80 nm, for exit from the ER and transport to the Golgi complex. To study the transport mechanism of procollagen IV, which generates basement membranes, we introduced a cysteine-free GFP tag at the N-terminus of the triple helical region of the α1(IV) chain (cfSGFP2-col4a1), and examined the dynamics of this protein in HT-1080 cells, which produce endogenous collagen IV. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20025DOI Listing

MON2 Guides Wntless Transport to the Golgi through Recycling Endosomes.

Cell Struct Funct 2020 Jun 12;45(1):77-92. Epub 2020 May 12.

Key Laboratory of Carbohydrate Chemistry, Ministry of Education, School of Biotechnology, Jiangnan University.

Endocytic cargos are transported to recycling endosomes (RE) but how these sorting platforms are generated is not well understood. Here we describe our biochemical and live imaging studies of the conserved MON2-DOPEY complex in RE formation. MON2 mainly co-localized with RE marker RAB4B in peripheral dots and perinuclear region. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20012DOI Listing

Roles of the Translation Initiation Factor eIF2α Phosphorylation in Cell Structure and Function.

Authors:
Sung Hoon Back

Cell Struct Funct 2020 Jun 29;45(1):65-76. Epub 2020 Apr 29.

School of Biological Sciences, University of Ulsan.

It is often assumed that α-subunit phosphorylation of the eukaryotic translation initiation factor 2 (eIF2) complex is just a mechanism to control protein synthesis. However, eIF2α phosphorylation induced by multiple kinases can recognize various intracellular and extracellular stress conditions, and it is involved in various other cellular processes beyond protein synthesis. This review introduces the roles of eIF2α phosphorylation in translational regulation, the generation of reactive oxygen species, changes in mitochondria structure and shape, and mitochondrial retrograde signaling pathways in response to diverse stress conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20013DOI Listing

Centrosomal and Non-centrosomal Functions Emerged through Eliminating Centrosomes.

Cell Struct Funct 2020 May 9;45(1):57-64. Epub 2020 Apr 9.

Department of Physiological Chemistry, Graduate School of Pharmaceutical Science, The University of Tokyo.

Centrosomes are highly conserved organelles that act as the major microtubule-organizing center (MTOC) in animal somatic cells. Through their MTOC activity, centrosomes play various roles throughout the cell cycle, such as supporting cell migration in interphase and spindle organization and positioning in mitosis. Various approaches for removing centrosomes from somatic cells have been developed and applied over the past few decades to understand the precise roles of centrosomes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.20007DOI Listing

Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes.

Cell Struct Funct 2020 Mar 7;45(1):45-55. Epub 2020 Feb 7.

Laboratory of Membrane Trafficking Mechanisms, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University.

Keratinocytes uptake melanosomes from melanocytes and retain them in the perinuclear region, where they form melanin caps. Although these processes are crucial to protecting nuclear DNA against ultraviolet injury, the molecular basis of melanosome uptake and decomposition in keratinocytes is poorly understood. One of the major reasons for its being poorly understood is the lack of a specific marker protein that can be used to visualize or monitor melanosomes (or melanosome-containing compartments) that have been incorporated into keratinocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19039DOI Listing

Hierarchical Development of Motile Polarity in Durotactic Cells Just Crossing an Elasticity Boundary.

Cell Struct Funct 2020 Feb 27;45(1):33-43. Epub 2019 Dec 27.

Laboratory of Biomedical and Biophysical Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University.

Cellular durotaxis has been extensively studied in the field of mechanobiology. In principle, asymmetric mechanical field of a stiffness gradient generates motile polarity in a cell, which is a driving factor of durotaxis. However, the actual process by which the motile polarity in durotaxis develops is still unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19040DOI Listing
February 2020

Development of a Rapid in vivo Assay to Evaluate the Efficacy of IRE1-specific Inhibitors of the Unfolded Protein Response Using Medaka Fish.

Cell Struct Funct 2020 Feb 26;45(1):23-31. Epub 2019 Dec 26.

Department of Biophysics, Graduate School of Science, Kyoto University.

Three types of transmembrane protein, IRE1α/IRE1β, PERK, and ATF6α/ATF6β, are expressed ubiquitously in vertebrates as transducers of the unfolded protein response (UPR), which maintains the homeostasis of the endoplasmic reticulum. IRE1 is highly conserved from yeast to mammals, and transmits a signal by a unique mechanism, namely splicing of mRNA encoding XBP1, the transcription factor downstream of IRE1 in metazoans. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19032DOI Listing
February 2020

Reinvestigation of Disulfide-bonded Oligomeric Forms of the Unfolded Protein Response Transducer ATF6.

Cell Struct Funct 2020 Jan 19;45(1):9-21. Epub 2019 Dec 19.

Department of Biophysics, Graduate School of Science, Kyoto University.

ATF6α is an endoplasmic reticulum (ER)-embedded transcription factor which is rapidly activated by ER stress, and a major regulator of ER chaperone levels in vertebrates. We previously suggested that ATF6α occurs as a monomer, dimer and oligomer in the unstressed ER of Chinese hamster ovary cells due to the presence of two evolutionarily conserved cysteine residues in its luminal region (C467 and C618), and showed that ATF6α is reduced upon ER stress, such that only reduced monomer ATF6α is translocated to the Golgi apparatus for activation by proteolysis. However, mutagenesis analysis (C467A and C618A) revealed that the C618A mutant behaves in an unexpected manner (monomer and oligomer) during non-reducing SDS-PAGE, for reasons which remained unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19030DOI Listing
January 2020

N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.

Cell Struct Funct 2020 Jan 30;45(1):1-8. Epub 2019 Nov 30.

Division of Biological Science, Graduate School of Science, Nagoya University.

The polytopic plasma membrane protein Rim21 senses both the elevation of ambient pH and alterations in plasma membrane lipid asymmetry in the Rim101 pathway in budding yeast. Rim21 is known to undergo N-glycosylation, but the site and function of the glycosylation modification is not known. Using a systematic mutation analysis, we found that Rim21 is N-glycosylated at an unconventional motif located in the N-terminal extracellular region. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19021DOI Listing
January 2020

Localization of BCR-ABL to Stress Granules Contributes to Its Oncogenic Function.

Cell Struct Funct 2019 Dec 15;44(2):195-204. Epub 2019 Nov 15.

Department of Cell Physiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University.

The oncogenic tyrosine kinase BCR-ABL activates a variety of signaling pathways and plays a causative role in the pathogenesis of chronic myelogenous leukemia (CML); however, the subcellular distribution of this chimeric protein remains controversial. Here, we report that BCR-ABL is localized to stress granules and that its granular localization contributes to BCR-ABL-dependent leukemogenesis. BCR-ABL-positive granules were not colocalized with any markers for membrane-bound organelles but were colocalized with HSP90a, a component of RNA granules. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19033DOI Listing
December 2019

Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins.

Cell Struct Funct 2019 Dec 15;44(2):183-194. Epub 2019 Nov 15.

Department of Cell Physiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University.

The discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we constructed a color pallet comprising different organelle markers and found that FFPs targeted to the mitochondria were mislocalized when fused to certain types of FPs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19028DOI Listing
December 2019

Dispersion of Endoplasmic Reticulum-associated Compartments by 4-phenyl Butyric Acid in Yeast Cells.

Cell Struct Funct 2019 Nov 17;44(2):173-182. Epub 2019 Oct 17.

Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology.

In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy. Here we show that a mutant of the cell surface protein Pma1, Pma1-2308, was accumulated in the ERACs, as well as the heterologously expressed mammalian cystic fibrosis transmembrane conductance regulator (CFTR), in yeast cells. Pma1-2308 and CFTR were located on the same ERACs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19023DOI Listing
November 2019
1 Read

Moss Kinesin-14 KCBP Accelerates Chromatid Motility in Anaphase.

Cell Struct Funct 2019 ;44(2):95-104

Division of Biological Science, Graduate School of Science, Nagoya University.

KCBP is a microtubule (MT) minus-end-directed kinesin widely conserved in plants. It was shown in Arabidopsis that KCBP controls trichome cell shape by orchestrating MT and actin cytoskeletons using its tail and motor domains. In contrast, the KCBP knockout (KO) line in the moss Physcomitrella patens showed a defect in nuclear and organelle positioning in apical stem cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19015DOI Listing

MGSE Regulates Crosstalk from the Mucin Pathway to the TFE3 Pathway of the Golgi Stress Response.

Cell Struct Funct 2019 Oct 19;44(2):137-151. Epub 2019 Oct 19.

Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo.

The Golgi apparatus is an organelle where membrane or secretory proteins receive post-translational modifications such as glycosylation and sulfation, after which the proteins are selectively transported to their final destinations through vesicular transport. When the synthesis of secretory or membrane proteins is increased and overwhelms the capacity of the Golgi (Golgi stress), eukaryotic cells activate a homeostatic mechanism called the Golgi stress response to augment the capacity of the Golgi. Four response pathways of the Golgi stress response have been identified, namely the TFE3, CREB3, HSP47, and proteoglycan pathways, which regulate the general function of the Golgi, apoptosis, cell survival, and proteoglycan glycosylation, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19009DOI Listing
October 2019

Mutations in mxc Tumor-Suppressor Gene Induce Chromosome Instability in Drosophila Male Meiosis.

Cell Struct Funct 2019 Oct 5;44(2):121-135. Epub 2019 Sep 5.

Department of Insect Biomedical Research, Center for Advanced Insect Research Promotion, Kyoto Institute of Technology.

Drosophila Mxc protein is a component of the histone locus body (HLB), which is required for the expression of canonical histone genes, and severe mxc mutations generate tumors in larval hematopoietic tissues. A common characteristic of cancer cells is chromosomal instability (CIN), but whether mxc mutants exhibit this feature is unknown. Here, examination of post-meiotic spermatids created after male meiosis revealed that a fraction of the spermatids in hypomorphic mxc mutants contained extra micronuclei or abnormally sized nuclei, corresponding to CIN. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19022DOI Listing
October 2019

Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Cell Struct Funct 2019 Sep 31;44(2):113-119. Epub 2019 Aug 31.

Division of Biological Science, Graduate School of Science, Nagoya University.

Every organism has a different set of genes essential for its viability. This indicates that an organism can become tolerant to the loss of an essential gene under certain circumstances during evolution, via the manifestation of 'masked' alternative mechanisms. In our quest to systematically uncover masked mechanisms in eukaryotic cells, we developed an extragenic suppressor screening method using haploid spores deleted of an essential gene in the fission yeast Schizosaccharomyces pombe. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877344PMC
September 2019

Implication of a Novel Function of Sar1 in the Nucleus.

Cell Struct Funct 2019 Sep 9;44(2):105-112. Epub 2019 Aug 9.

Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo.

The coat protein complex II (COPII) generates transport carriers that deliver newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. The small GTPase Sar1 is a well-known regulator of the assembly of the COPII coat. In the present study, we demonstrate that, besides its well-established role in ER-to-Golgi trafficking, the nuclear localization of Sar1 is essential for the viability of Saccharomyces cerevisiae. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19019DOI Listing
September 2019

Towards Enhancing Therapeutic Glycoprotein Bioproduction: Interventions in the PI3K/AKT/mTOR Pathway.

Cell Struct Funct 2019 ;44(2):75-83

Institute for Drug Research, The Hebrew University of Jerusalem.

Recombinant glycoproteins produced in mammalian cells are clinically indispensable drugs used to treat a broad spectrum of diseases. Their bio-manufacturing process is laborious, time consuming, and expensive. Investment in expediting the process and reducing its cost is the subject of continued research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19013DOI Listing

Organelle Zones.

Cell Struct Funct 2019 Aug 12;44(2):85-94. Epub 2019 Jul 12.

Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo.

In research on cell biology, organelles have been a major unit of such analyses. Researchers have assumed that the inside of an organelle is almost uniform in regards to its function, even though each organelle has multiple functions. However, we are now facing conundrums that cannot be resolved so long as we regard organelles as functionally uniform units. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.19010DOI Listing

A Peptide Derived from Phosphoinositide 3-kinase Inhibits Endocytosis and Influenza Virus Infection.

Cell Struct Funct 2019 Apr 21;44(1):61-74. Epub 2019 Mar 21.

Department of Cell Physiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University.

Endocytosis mediates the internalization and ingestion of a variety of endogenous or exogenous substances, including virus particles, under the control of intracellular signaling pathways. We have previously reported that the complex formed between the small GTPase Ras and phosphoinositide 3-kinase (PI3K) translocates from the plasma membrane to endosomes, signaling from which thereby regulates clathrin-independent endocytosis, endosome maturation, influenza virus internalization, and infection. However, the molecular mechanism by which the Ras-PI3K complex is recruited to endosomes remains unclear. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/advpub/0/advpub_190
Publisher Site
http://dx.doi.org/10.1247/csf.19001DOI Listing
April 2019
12 Reads

Two Decades of Genetically Encoded Biosensors Based on Förster Resonance Energy Transfer.

Cell Struct Funct 2019 Nov 21;44(2):153-169. Epub 2019 Mar 21.

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University.

Two decades have passed since the development of the first calcium indicator based on the green fluorescent protein (GFP) and the principle of Förster resonance energy transfer (FRET). During this period, researchers have advanced many novel ideas for the improvement of such genetically encoded FRET biosensors, which have allowed them to expand their targets from small molecules to signaling proteins and physicochemical properties. Although the merits of "genetically encoded" FRET biosensors became clear once various cell lines were established and several transgenic organisms were generated, the road to these developments was not necessarily a smooth one. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18035DOI Listing
November 2019

Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p in Retinoblastoma.

Cell Struct Funct 2019 ;44(1):51-60

Department of Ophthalmology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University.

Retinoblastoma (RB) is the most common intraocular malignancy in childhood, and the prognosis in the advanced RB is poor. It is urgent to find novel therapeutic targets. Long noncoding RNAs (lncRNAs) have critical functions in cancer progression, and lncRNA LINC00202 is found associated with poor prognosis in RB. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18033DOI Listing
August 2019
1 Read

PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression.

Cell Struct Funct 2019 ;44(1):29-39

Department of Neurosurgery, the Second Hospital of Hebei Medical University.

PUM2, an RNA binding protein, is known to promote stem cell proliferation via repressing expressions of cell cycle genes. Similar with stem cells, malignant cells are characterized by unlimited proliferation and remote migration. However, roles of PUM2 in cancer development are controversial. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18030DOI Listing
August 2019
2 Reads

CCL20 Promotes Ovarian Cancer Chemotherapy Resistance by Regulating ABCB1 Expression.

Cell Struct Funct 2019 ;44(1):21-28

Department of Ultrasound, the Central Hospital of Zibo.

Ovarian cancer (OC) is one of prevalent tumors and this study aimed to explore CCL20's effects on doxorubicin resistance of OC and related mechanisms. Doxorubicin-resistant SKOV3 DR cells were established from SKOV3 cells via 6-month continuous exposure to gradient concentrations of doxorubicin. Quantitative PCR and Western blot assay showed that SKOV3 DR cells had higher level of CCL20 than SKOV3 cells, and doxorubicin upregulated CCL20 expression in SKOV3 cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18029DOI Listing
August 2019
3 Reads

MiR-187-3p Enhances Propranolol Sensitivity of Hemangioma Stem Cells.

Cell Struct Funct 2019 Mar 2;44(1):41-50. Epub 2019 Feb 2.

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine.

Infantile hemangioma is the most common soft tissue tumors in childhood. In clinic, propranolol is widely used for infantile hemangioma therapy. However, some of the infantile hemangioma patients display resistance to propranolol treatment. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/advpub/0/advpub_180
Publisher Site
http://dx.doi.org/10.1247/csf.18041DOI Listing
March 2019
40 Reads
1.684 Impact Factor

PGSE Is a Novel Enhancer Regulating the Proteoglycan Pathway of the Mammalian Golgi Stress Response.

Cell Struct Funct 2019 Jan 28;44(1):1-19. Epub 2018 Nov 28.

Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo.

The Golgi stress response is a homeostatic mechanism that augments the functional capacity of the Golgi apparatus when Golgi function becomes insufficient (Golgi stress). Three response pathways of the Golgi stress response have been identified in mammalian cells, the TFE3, HSP47 and CREB3 pathways, which augment the capacity of specific Golgi functions such as N-glycosylation, anti-apoptotic activity and pro-apoptotic activity, respectively. On the contrary, glycosylation of proteoglycans (PGs) is another important function of the Golgi, although the response pathway upregulating expression of glycosylation enzymes for PGs in response to Golgi stress remains unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18031DOI Listing
January 2019
2 Reads

Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.

Cell Struct Funct 2018 ;43(2):177-185

Faculty of Advanced Life Science, Hokkaido University.

Glycans, including glycosphingolipids, are broadly expressed in plasma membranes and play important roles in cell-cell interactions. Recently, it has been revealed that glycans participate in the regulation of malignant phenotypes of cancer cells, e.g. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/43/2/43_18026/_arti
Publisher Site
http://dx.doi.org/10.1247/csf.18026DOI Listing
February 2019
24 Reads

An Anti-tumorigenic Role of the Warburg Effect at Emergence of Transformed Cells.

Cell Struct Funct 2018 Sep 26;43(2):171-176. Epub 2018 Jul 26.

Division of Development and Aging, Research Institute for Biomedical Sciences, Tokyo University of Science.

The Warburg effect is one of the hallmarks of cancer cells, characterized by enhanced aerobic glycolysis. Despite intense research efforts, its functional relevance or biological significance to facilitate tumor progression is still debatable. Hence the question persists when and how the Warburg effect contributes to carcinogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18018DOI Listing
September 2018
3 Reads

Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation.

Cell Struct Funct 2018 Aug 26;43(2):153-169. Epub 2018 Jul 26.

Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo.

Automatic cell segmentation is a powerful method for quantifying signaling dynamics at single-cell resolution in live cell fluorescence imaging. Segmentation methods for mononuclear and round shape cells have been developed extensively. However, a segmentation method for elongated polynuclear cells, such as differentiated C2C12 myotubes, has yet to be developed. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/43/2/43_18012/_arti
Publisher Site
http://dx.doi.org/10.1247/csf.18012DOI Listing
August 2018
3 Reads

Branched Sialylated N-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H.

Cell Struct Funct 2018 Aug 20;43(2):141-152. Epub 2018 Jul 20.

Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, National Institutes of Natural Sciences.

Proper N-glycosylation of proteins is important for normal brain development and nervous system function. Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins. The present study examined the N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-). Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/43/2/43_18009/_arti
Publisher Site
http://dx.doi.org/10.1247/csf.18009DOI Listing
August 2018
47 Reads

Mesoporous Silica Nanoparticles Rescue HO-induced Inhibition of Cardiac Differentiation.

Cell Struct Funct 2018 ;43(2):109-117

Department of Cardiovascular Surgery, Peking University Shenzhen Hospital.

The anti-oxidative property of mesoporous silica nanoparticles (MSNs) has been proposed previously, which prompted us to investigate the potential protective effect of MSNs on human embryonic stem cells (hESCs) against oxidative stress. To this purpose, the cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Apoptosis was analyzed by Annexin V/propidium iodide double-staining method. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/43/2/43_18008/_arti
Publisher Site
http://dx.doi.org/10.1247/csf.18008DOI Listing
November 2018
24 Reads

A Novel Morphological Marker for the Analysis of Molecular Activities at the Single-cell Level.

Cell Struct Funct 2018 Aug 29;43(2):129-140. Epub 2018 Jun 29.

Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University.

For more than a century, hematoxylin and eosin (H&E) staining has been the de facto standard for histological studies. Consequently, the legacy of histological knowledge is largely based on H&E staining. Due to the recent advent of multi-photon excitation microscopy, the observation of live tissue is increasingly being used in many research fields. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18013DOI Listing
August 2018
50 Reads

Detailed Analysis of the Interaction of Yeast COG Complex.

Cell Struct Funct 2018 Jul 14;43(2):119-127. Epub 2018 Jun 14.

Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics.

The Golgi apparatus is a central station for protein trafficking in eukaryotic cells. A widely accepted model of protein transport within the Golgi apparatus is cisternal maturation. Each cisterna has specific resident proteins, which are thought to be maintained by COPI-mediated transport. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116515PMC
July 2018
30 Reads
1.684 Impact Factor

Long-term Culture of Human iPS Cell-derived Telencephalic Neuron Aggregates on Collagen Gel.

Cell Struct Funct 2018 ;43(1):85-94

Faculty of Advanced Life Science, Hokkaido University.

It takes several months to form the 3-dimensional morphology of the human embryonic brain. Therefore, establishing a long-term culture method for neuronal tissues derived from human induced pluripotent stem (iPS) cells is very important for studying human brain development. However, it is difficult to keep primary neurons alive for more than 3 weeks in culture. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18002DOI Listing
October 2018
4 Reads

Solo and Keratin Filaments Regulate Epithelial Tubule Morphology.

Cell Struct Funct 2018 Jun 28;43(1):95-105. Epub 2018 Apr 28.

Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University.

Epithelial tubules, consisting of the epithelial cell sheet with a central lumen, are the basic structure of many organs. Mechanical forces play an important role in epithelial tubulogenesis; however, little is known about the mechanisms controlling the mechanical forces during epithelial tubule morphogenesis. Solo (also known as ARHGEF40) is a RhoA-targeting guanine-nucleotide exchange factor that is involved in mechanical force-induced RhoA activation and stress fiber formation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18010DOI Listing
June 2018
4 Reads

Hidden Peptides Encoded by Putative Noncoding RNAs.

Cell Struct Funct 2018 May 11;43(1):75-83. Epub 2018 Apr 11.

Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University.

Although the definition of a noncoding RNA (ncRNA) is an RNA molecule that does not encode a protein, recent evidence has revealed that some ncRNAs are indeed translated to give rise to small polypeptides (usually containing fewer than 100 amino acids). Despite their small size, however, these peptides are often biologically relevant in that they are required for a variety of cellular processes. In this review, we summarize the production and functions of peptides that have been recently identified as translation products of putative ncRNAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18005DOI Listing
May 2018
17 Reads

Live-cell Imaging with Genetically Encoded Protein Kinase Activity Reporters.

Cell Struct Funct 2018 Apr 17;43(1):61-74. Epub 2018 Mar 17.

Division of Quantitative Biology, Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences.

Protein kinases play pivotal roles in intracellular signal transduction, and dysregulation of kinases leads to pathological results such as malignant tumors. Kinase activity has hitherto been measured by biochemical methods such as in vitro phosphorylation assay and western blotting. However, these methods are less useful to explore spatial and temporal changes in kinase activity and its cell-to-cell variation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18003DOI Listing
April 2018
3 Reads

Structural Insights into the Altering Function of CRMP2 by Phosphorylation.

Cell Struct Funct 2018 ;43(1):15-23

Division of Structural Medicine and Anatomy, Kobe University Graduate School of Medicine.

Collapsin response mediator protein 2 (CRMP2) regulates neuronal polarity by controlling microtubule dynamics. CRMP2 activity is regulated by semaphorin-induced phosphorylation at the C-terminal tail domain. Unphosphorylated CRMP2 induces effective axonal microtubule formation to give the axonal characteristics to a neurite, whereas phosphorylated CRMP2 leads to the apparently opposite effect, growth cone collapse. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.17025DOI Listing
October 2018
1 Read

SPIRAL2 Stabilises Endoplasmic Microtubule Minus Ends in the Moss Physcomitrella patens.

Cell Struct Funct 2018 03 15;43(1):53-60. Epub 2018 Mar 15.

Division of Biological Science, Graduate School of Science, Nagoya University.

Stabilisation of minus ends of microtubules (MTs) is critical for organising MT networks in land plant cells, in which all MTs are nucleated independent of centrosomes. Recently, Arabidopsis SPIRAL2 (SPR2) protein was shown to localise to plus and minus ends of cortical MTs, and increase stability of both ends. Here, we report molecular and functional characterisation of SPR2 of the basal land plant, the moss Physcomitrella patens. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.18001DOI Listing
March 2018
3 Reads

A Missense Mutation in the NSF Gene Causes Abnormal Golgi Morphology in Arabidopsis thaliana.

Cell Struct Funct 2018 Mar 2;43(1):41-51. Epub 2018 Mar 2.

Department of Biological Sciences, Graduate School of Science, The University of Tokyo.

The Golgi apparatus is a key station of glycosylation and membrane traffic. It consists of stacked cisternae in most eukaryotes. However, the mechanisms how the Golgi stacks are formed and maintained are still obscure. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/43/1/43_17026/_arti
Publisher Site
http://dx.doi.org/10.1247/csf.17026DOI Listing
March 2018
4 Reads

Intestinal Epithelial Cell-specific Deletion of α-Mannosidase II Ameliorates Experimental Colitis.

Cell Struct Funct 2018 Mar 18;43(1):25-39. Epub 2018 Jan 18.

Division of Biochemistry, Faculty of Pharmacy, Keio University.

Inflammatory bowel disease (IBD) is a refractory disease of the gastrointestinal tract that is believed to develop in genetically susceptible individuals. Glycosylation, a type of post-translational modification, is involved in the development of a wide range of diseases, including IBD, by modulating the function of various glycoproteins. To identify novel genes contributing to the development of IBD, we analyzed single nucleotide polymorphisms (SNPs) of glycosylation-related genes in IBD patients and identified MAN2A1, encoding alpha-mannosidase II (α-MII), as a candidate gene. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.17022DOI Listing
March 2018
10 Reads

Flagellar-associated Protein FAP85 Is a Microtubule Inner Protein That Stabilizes Microtubules.

Cell Struct Funct 2018 Feb 28;43(1):1-14. Epub 2017 Dec 28.

Department of Picobiology, Graduate School of Life Science, University of Hyogo.

Genomics and proteomics studies in Chlamydomonas have revealed that an axoneme is composed of 200-600 types of proteins, including uncharacterized proteins collectively named flagellar-associated proteins (FAPs). Nine FAPs contain the EF-hand motif; however, they have not yet been well characterized. To find components responsible for Chlamydomonas-specific waveform changes coupled with intracellular Ca concentrations, we focused on FAP85, an EF-hand motif-containing FAP specific to Chlamydomonas and its relatives. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.17023DOI Listing
February 2018
97 Reads

Expression of Ror2 Associated with Fibrosis of the Submandibular Gland.

Cell Struct Funct 2017 Dec 25;42(2):159-167. Epub 2017 Oct 25.

Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine.

The submandibular gland (SMG) is one of the major salivary glands that play important roles for variety of physiological functions, such as digestion of foods, prevention of infection, and lubrication of the mouth. Dysfunction of the SMG, often associated with a salivary inflammation, adversely influences a person's quality of life. However, the mechanism underlying inflammation-driven dysfunction of the SMG is largely unknown. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/csf/42/2/42_17019/_arti
Publisher Site
http://dx.doi.org/10.1247/csf.17019DOI Listing
December 2017
7 Reads

Bone Marrow Endothelial Cells Induce Immature and Mature B Cell Egress in Response to Erythropoietin.

Cell Struct Funct 2017 Dec 25;42(2):149-157. Epub 2017 Oct 25.

Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University.

Bone marrow stromal cells, including endothelial cells and mesenchymal stromal cells, support the maintenance, differentiation, and retention of hematopoietic stem and precursor cells under steady state conditions. At the onset of an emergency, such as severe blood loss or infection, the status of hematopoiesis in the bone marrow changes rapidly to ensure efficient production of cells of specific lineages; however, the function of stromal cells in emergency hematopoiesis has not been fully elucidated. Here, we unexpectedly found that B precursor, mature B, and T cells were released from the bone marrow into the blood circulation in the early phase of hemorrhagic anemia and phenylhydrazine-induced hemolytic anemia. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1247/csf.17018DOI Listing
December 2017
3 Reads