142 results match your criteria Cell medicine[Journal]


Mitochondrial Respiratory Capacity is Restored in Hibernating Cardiomyocytes Following Co-Culture with Mesenchymal Stem Cells.

Cell Med 2019 6;11:2155179019834938. Epub 2019 Mar 6.

Research Service, Minneapolis VA Health Care System, USA.

Hibernating myocardium is a subset of ischemic cardiac disease characterized by viable but dysfunctional tissue. Standard treatment for hibernating myocardium is coronary artery bypass graft, which reduces arrhythmias and improves survival but does not fully restore function, presenting a gap in currently available treatments. Large animal studies of hibernating myocardium have identified impaired mitochondrial dynamics as a root cause of persistent cardiac dysfunction despite surgical revascularization. Read More

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http://dx.doi.org/10.1177/2155179019834938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404044PMC

Approval of First CAR-Ts: Have we Solved all Hurdles for ATMPs?

Cell Med 2019 22;11:2155179018822781. Epub 2019 Jan 22.

Shire, Zug, Switzerland.

T cells are known as the most potent killer cells of the immune system, designed by nature to prevent unwanted challenges. The first class of therapeutic products harnessing the power of T cells for target-specific treatment of oncological diseases was bispecific antibodies. The first T-cell engaging bispecific antibodies that obtained approval were catumaxomab and blinatumomab. Read More

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http://dx.doi.org/10.1177/2155179018822781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343443PMC
January 2019

Risk Factors for Perinatal Arterial Ischemic Stroke: A Case-Control Study.

Cell Med 2018 11;10:2155179018785341. Epub 2018 Jul 11.

Department of Pediatric Neurology and Psychiatry, San Borja Arriarán Hospital, University of Chile, Santiago, Chile.

Introduction: Arterial ischemic stroke in newborns is an important cause of neonatal morbidity and mortality. Its pathophysiology and associated risk factors are not yet clearly understood and defined.

Objective: The aim of this retrospective study was to investigate possible risk factors in diagnosed cases of PAIS (perinatal arterial ischemic stroke). Read More

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http://dx.doi.org/10.1177/2155179018785341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172995PMC

Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification.

Cell Med 2018 29;10:2155179018781343. Epub 2018 Jun 29.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. Read More

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http://dx.doi.org/10.1177/2155179018781343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172984PMC

A Comparison of Pancreatic Islet Purification using Iodixanol with University of Wisconsin Solution and with Na-Lactobionate and Histidine Solution.

Cell Med 2018 7;10:2155179018775071. Epub 2018 Jun 7.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa Prefecture, Japan.

Purification of pancreatic islets is an important step in islet isolation for islet transplantation. In this study, to investigate how a solution composed mainly of Na-lactobionate and histidine (HL) influences the purification of islets, iodixanol was added to a purified solution for porcine islet isolation. A solution (IU) made by adding iodixanol to University of Wisconsin solution and a solution (IHL) made by adding iodixanol to HL solution were used to evaluate the islet isolation performance. Read More

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http://dx.doi.org/10.1177/2155179018775071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172993PMC

3-D retina organoids: Building platforms for therapies of the future.

Cell Med 2018 8;10:2155179018773758. Epub 2018 Jun 8.

National Eye Institute, National Institutes of Health, Bethesda, USA.

Across scientific disciplines, 3-D organoid culture systems offer platforms to integrate basic research findings with clinical care. The National Eye Institute mounted a $1.1 million 3-D Retina Organoid Challenge. Read More

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http://dx.doi.org/10.1177/2155179018773758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172996PMC

The effect of age on stem cell function and utility for therapy.

Authors:
Patrick Narbonne

Cell Med 2018 8;10:2155179018773756. Epub 2018 Jun 8.

Département de Biologie Médicale, Université du Québec à Trois-Rivières, Canada.

During development, stem cells generate all of the differentiated cells that populate our tissues and organs. Stem cells are also responsible for tissue turnover and repair in adults, and as such, they hold tremendous promise for regenerative therapy. Aging, however, impairs the function of stem cells and is thus a significant roadblock to using stem cells for therapy. Read More

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http://dx.doi.org/10.1177/2155179018773756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172989PMC

Pediatric Arterial Ischemic Stroke: Clinical Presentation, Risk Factors, and Pediatric NIH Stroke Scale in a Series of Chilean Patients.

Cell Med 2018 29;10:2155179018760330. Epub 2018 May 29.

Department of Pediatric Neurology and Psychiatry, San Borja Arriarán Hospital, University of Chile, Santiago, Región Metropolitana, Chile.

Stroke is an important cause of morbidity and mortality in children. Clinical presentation is diverse, and multiple risk factors have been described. The aim of this retrospective study is to describe the clinical presentation, risk factors, and the Pediatric National Institute of Health Stroke Scale (PedNIHSS) in a series of pediatric Chilean patients with the diagnosis of arterial ischemic stroke (AIS). Read More

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http://dx.doi.org/10.1177/2155179018760330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172992PMC

Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model.

Cell Med 2018 31;10:2155179018760327. Epub 2018 May 31.

Department of Integrative Medical Biology, Section for Anatomy, Umeå University, Umeå, Sweden.

Introduction: Previously we showed that a fibrin glue conduit with human mesenchymal stem cells (hMSCs) and cyclosporine A (CsA) enhanced early nerve regeneration. In this study long term effects of this conduit are investigated.

Methods: In a rat model, the sciatic nerve was repaired with fibrin conduit containing fibrin matrix, fibrin conduit containing fibrin matrix with CsA treatment and fibrin conduit containing fibrin matrix with hMSCs and CsA treatment, and also with nerve graft as control. Read More

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http://dx.doi.org/10.1177/2155179018760327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172997PMC

Airway Delivery of Bone Marrow-Derived Mesenchymal Stem Cells Reverses Bronchopulmonary Dysplasia Superimposed with Acute Respiratory Distress Syndrome in an Infant.

Cell Med 2018 18;10:2155179018759434. Epub 2018 Jun 18.

Division of Cardiovascular Surgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.

Bronchopulmonary dysplasia (BPD), a disease affecting extremely premature infants, results from the disruption of normal pulmonary vascular and alveolar growth. Currently, there is no specific effective treatment. We report a case of a 10-mo-old female infant with BPD, who was admitted because of adenovirus pneumonia and acute respiratory distress syndrome (ARDS) with prolonged venovenous and arteriovenous extracorporeal membrane oxygenation (ECMO) support (total 125 d). Read More

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http://dx.doi.org/10.1177/2155179018759434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172994PMC

Human Cells Grown With or Without Substitutes for Fetal Bovine Serum.

Cell Med 2018 6;10:2155179018755140. Epub 2018 Jun 6.

Department of Biology, Mississippi College, Clinton, MS, USA.

Safety concerns over cell-derived pharmaceutical products being manufactured in supplements of fetal bovine serum (FBS) have ignited pleas to replace FBS. Herein, four newly marketed alternatives to FBS were compared: a xeno-free product called Cell-Ess®, a human platelet lysate marketed as GroPro®, and two mixtures of adult bovine serum varying in their proportions of neonatal growth factors, called Liporo® and FetalGro®. An endothelial cell line (C2BBe1) and a neuronal cell line (SHSY5Y) near confluency in media with 10% FBS were selectively scraped and taken through a 25-day step-wise algorithm to replace FBS, and another human endothelial cell line (HRA-19) was studied to replicate C2BBe1. Read More

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http://dx.doi.org/10.1177/2155179018755140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172986PMC

Role of Egr1 on Pancreatic Endoderm Differentiation.

Cell Med 2018 29;10:2155179017733177. Epub 2018 May 29.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

The low efficiency of in vitro differentiation of human embryonic stem cells (hESCs) or human-induced pluripotent stem cells (iPSCs) into insulin-producing cells is a crucial hurdle for the clinical implementation of human pluripotent stem cells (PSCs). Our previous investigation into the key factors for the differentiation of PSCs into insulin-producing cells suggested that the expression of GATA binding protein 6 (GATA6) and Gremlin 1 (GREM1) and inhibition of early growth response protein 1 (Egr1) may be important factors. In this study, we investigated the role of Egr1 in pancreas development. Read More

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http://dx.doi.org/10.1177/2155179017733177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172987PMC

The Development of Cancer through the Transient Overexpression of Reprogramming Factors.

Cell Med 2018 29;10:2155179017733172. Epub 2018 May 29.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

Although induced pluripotent stem (iPS) cells have significant implications for overcoming most of the ethical issues associated with embryonic stem cells, several issues related to the use of iPS cells in clinical applications remain unresolved, including the issue of teratoma formation. We previously reported that the induction of induced tissue-specific stem (iTS) cells from the pancreas (iTS-P) or liver (iTS-L) by the transient overexpression of reprogramming factors, combined with tissue-specific selection and the generation of iTS cells, could have important implications for the clinical application of stem cells. At the same time, we also generated "induced fibroblast-like (iF) cells" that were capable of self-renewal, which had a similar morphology to fibroblast cells. Read More

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http://dx.doi.org/10.1177/2155179017733172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172998PMC

Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion.

Cell Med 2018 29;10:2155179017733152. Epub 2018 May 29.

Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan.

Positioning single cells on a solid surface is a crucial technique for understanding the cellular functions and cell-cell interactions in cell culture assays. We developed a microfluidic chip for depositing single cells in microwells using a simple micropipette operation. Cells were delivered to microwells by the meniscus motion of liquid interface. Read More

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http://dx.doi.org/10.1177/2155179017733152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172985PMC

Long-term Cryopreservation of Human and other Mammalian Cells at -80 °C for 8 Years.

Cell Med 2018 29;10:2155179017733148. Epub 2018 May 29.

Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

Freezing is recognized as the most effective method of maintaining a stable supply of various cell types for long-term storage. However, cells might be damaged by environmental changes during the freezing process. There are various factors that influence the function of cells cultured after cryopreservation and thawing. Read More

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http://dx.doi.org/10.1177/2155179017733148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172990PMC

Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue.

Cell Med 2018 29;10:2155179017733090. Epub 2018 May 29.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. Read More

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http://dx.doi.org/10.1177/2155179017733090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172999PMC

Therapeutic Cocktail Approach for Treatment of Hyperhomocysteinemia in Alzheimer's Disease.

Cell Med 2018 25;10:2155179017722280. Epub 2018 Jan 25.

Department of Psychiatry and Behavioral Neurosciences, Rashid Laboratory for Developmental Neurobiology, Silver Child Development Center, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.

In the United States, Alzheimer's disease (AD) is the most common cause of dementia, accompanied by substantial economic and emotional costs. During 2015, more than 15 million family members who provided care to AD patients had an estimated total cost of 221 billion dollars. Recent studies have shown that elevated total plasma levels of homocysteine (tHcy), a condition known as hyperhomocysteinemia (HHcy), is a risk factor for AD. Read More

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http://dx.doi.org/10.1177/2155179017722280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172991PMC
January 2018

A Case of Acute Lymphocytic Leukaemia with t(3;13) and Central Nervous System Leukemia after Allogenic Cord Blood Transplantation.

Cell Med 2019 4;11:2155179019873850. Epub 2019 Sep 4.

Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, P.R. China.

Background: Acute lymphoblastic leukemia (ALL) is a neoplastic cancer characterized by clonal expansion of leukemic cells in lymph organs and bone marrow. Lots of kinds of different chromosomal translocations can be found in those leukemic cells. However, the role of abnormal chromosomes and genes in leukemogenesis is not yet fully understood. Read More

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http://dx.doi.org/10.1177/2155179019873850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728670PMC
September 2019

MicroRNAs as Key Regulators of Ovarian Cancers.

Cell Med 2019 6;11:2155179019873849. Epub 2019 Sep 6.

Department of Zoology, University of Delhi, Delhi, India.

The tumor microenvironment can be realistically viewed as an active battle ground between the host immune system and the growing tumor cells. This reactive space surrounding the tumor possesses several possibilities and facilitates the progression of a tumor from a neoplastic stage to that of metastasis. The contemporary approach of understanding the cancer biology from a "within the cell" perspective has been largely challenged with complex and intricate "outside the cell" events. Read More

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http://dx.doi.org/10.1177/2155179019873849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732848PMC
September 2019

Unsurpassed Intrahepatic Islet Engraftment - the Quest for New Sites for Beta Cell Replacement.

Cell Med 2019 24;11:2155179019857662. Epub 2019 Jun 24.

Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.

The liver is currently the site of choice for clinical islet transplantation, even though many alternative implantation sites have lately been proposed as more ideal for graft survival. The suggested sites, for example intramuscular space, omentum, bone marrow, and spleen, are sometimes difficult to compare due to differences in animal model, islet isolation procedure, and islet quality. In addition, the variation in transplanted islet mass is vast. Read More

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http://dx.doi.org/10.1177/2155179019857662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593927PMC

Bone Grafts, Bone Substitutes and Regenerative Medicine Acceptance for the Management of Bone Defects Among French Population: Issues about Ethics, Religion or Fear?

Cell Med 2019 20;11:2155179019857661. Epub 2019 Jun 20.

INSERM (French National Institute of Health and Medical Research), UMR1260, Regenerative Nanomedicine (RNM), FMTS.

Several techniques exist to manage bone defects in patients: bone grafts (autograft, allograft, xenograft), use of synthetic bone substitutes, or use of the products of bone regenerative medicine. Studies generally focus on their efficacy, but few focus on their acceptance. Our objectives were to assess their theoretical acceptance among the French general population, and to identify issues justifying refusals, by mean of an open e-questionnaire. Read More

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http://dx.doi.org/10.1177/2155179019857661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587382PMC

Higher Serum Alanine Transaminase Levels in Male Urokinase-Type Plasminogen Activator-Transgenic Mice Are Associated With Improved Engraftment of Hepatocytes but not Liver Sinusoidal Endothelial Cells.

Cell Med 2017 23;9(3):117-125. Epub 2016 Nov 23.

Blood Systems Research Institute, San Francisco, CA, USA.

The effects of sex on the degree of liver damage and human cell engraftment were investigated in immunodeficient urokinase-type plasminogen activator-transgenic (uPA-NOG) mice. Liver damage, measured by serum alanine transaminase (ALT) levels, was compared in male and female uPA-NOG mice of different ages. Male mice had significantly higher ALT levels than females with a median of 334 versus 158 U/L in transgenic homozygous mice, respectively. Read More

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http://dx.doi.org/10.3727/215517916X693375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509021PMC
November 2016
34 Reads

Human Adipose-Derived Mesenchymal Stem Cells Respond to Short-Term Hypoxia by Secreting Factors Beneficial for Human Islets In Vitro and Potentiate Antidiabetic Effect In Vivo.

Cell Med 2017 14;9(3):103-116. Epub 2017 Apr 14.

Department of Transplant Medicine, Oslo University Hospital, Oslo, Norway.

Adipose-derived mesenchymal stem cells (ASCs) release factors beneficial for islets in vitro and protect against hyperglycemia in rodent models of diabetes. Oxygen tension has been shown to induce metabolic changes and alter ASCs' release of soluble factors. The effects of hypoxia on the antidiabetic properties of ASCs have not been explored. Read More

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http://dx.doi.org/10.3727/215517917X693401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509020PMC
April 2017
30 Reads

A Prospective, Nonrandomized, no Placebo-Controlled, Phase I/II Clinical Trial Assessing the Safety and Efficacy of Intramuscular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells in Patients With Severe Buerger's Disease.

Cell Med 2017 3;9(3):87-102. Epub 2016 Oct 3.

Biostar Stem Cell Research Institute, R Bio Co. Ltd., Seoul, Republic of Korea.

Buerger's disease is a rare and severe disease affecting the blood vessels of the limbs. Adipose tissue-derived mesenchymal stem cells (ADSCs) have the potential to cure Buerger's disease when developed as a stem cell drug. In the present study, we conducted a prospective, nonrandomized, no placebo-controlled, phase I/II clinical trial with a 2-year follow-up questionnaire survey. Read More

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http://dx.doi.org/10.3727/215517916X693069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509019PMC
October 2016
3 Reads

Neurovascular Cell Sheet Transplantation in a Canine Model of Intracranial Hemorrhage.

Cell Med 2017 21;9(3):73-85. Epub 2016 Dec 21.

Department of Neurology, Seoul National University Hospital, College of Medicine, Seoul National University, Seoul, South Korea.

Cell-based therapy for intracerebral hemorrhage (ICH) has a great therapeutic potential. However, methods to effectively induce direct regeneration of the damaged neural tissue after cell transplantation have not been established, which, if done, would improve the efficacy of cell-based therapy. In this study, we aimed to develop a cell sheet with neurovasculogenic potential and evaluate its usefulness in a canine ICH model. Read More

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http://dx.doi.org/10.3727/215517916X693384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509018PMC
December 2016
26 Reads

Regenerative Rehabilitation: An Innovative and Multifactorial Approach to Recovery From Stroke and Brain Injury.

Cell Med 2017 14;9(3):67-71. Epub 2017 Apr 14.

Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.

There is currently a dearth of treatment options for stroke or traumatic brain injury that can restore cognitive and motor function. Regenerative and translational medicine have ushered forth promising new methods for mediating recovery in the central nervous system, the most salient of which are rehabilitation and stem cell therapies that, when combined, result in more pronounced recovery than one approach alone. Read More

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http://dx.doi.org/10.3727/215517917X693393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509017PMC
April 2017
14 Reads

Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching.

Cell Med 2017 Jan 14;9(1-2):61-66. Epub 2016 Sep 14.

Institute of Innovation for Future Society, Nagoya University, Chikusa-ku, Nagoya, Japan; †ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan; ¶Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.

Abnormal DNA methylation in CpG-rich promoters is recognized as a distinct molecular feature of precursor lesions to cancer. Such unintended methylation can occur during in vitro differentiation of stem cells. It takes place in a subset of genes during the differentiation or expansion of stem cell derivatives under general culture conditions, which may need to be monitored in future cell transplantation studies. Read More

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http://dx.doi.org/10.3727/215517916X693087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225679PMC
January 2017
17 Reads

Comparison of Purification Solutions With Different Osmolality for Porcine Islet Purification.

Cell Med 2017 Jan 1;9(1-2):53-59. Epub 2016 Sep 1.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.

The osmolality of the purification solution is one of the most critical variables in human islet purification during islet isolation. We previously reported the effectiveness of a combined continuous density/osmolality gradient for the supplemental purification of human islets. We herein applied a combined continuous density/osmolality gradient for regular purification. Read More

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http://dx.doi.org/10.3727/215517916X693140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225678PMC
January 2017
29 Reads

The Evaluation of Islet Purification Methods That Use Large Bottles to Create a Continuous Density Gradient.

Cell Med 2017 Jan 1;9(1-2):45-51. Epub 2016 Sep 1.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.

Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. The most common method of islet purification is density gradient centrifugation using a COBE 2991 cell processor. However, this method can damage islets mechanically through its high shearing force. Read More

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http://dx.doi.org/10.3727/215517916X693131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225677PMC
January 2017
23 Reads

Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an In Vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture.

Cell Med 2017 Jan 14;9(1-2):35-44. Epub 2016 Sep 14.

Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine , Showa-ku, Nagoya , Japan.

The application of stem cells for cell therapy has been extensively studied in recent years. Among the various types of stem cells, human adipose tissue-derived stem cells (ASCs) can be obtained in large quantities with relatively few passages, and they possess a stable quality. ASCs can differentiate into a number of cell types, such as adipose cells and ectodermal cells. Read More

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http://dx.doi.org/10.3727/215517916X693096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225676PMC
January 2017
6 Reads

Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice.

Cell Med 2017 Jan 6;9(1-2):21-33. Epub 2016 Oct 6.

Department of Radiological and Medical Laboratory Sciences, Nagoya University Graduate School of Medicine , Higashi-ku, Nagoya , Japan.

Cell therapy with adipose tissue-derived stem cells (ASCs) is expected to be a candidate for the treatment of fulminant hepatic failure (FHF), which is caused by excessive immune responses. In order to evaluate the therapeutic effects of ASCs on FHF, the in vitro and in vivo immunomodulatory effects of ASCs were examined in detail in the mouse model. The in vitro effects of ASCs were examined by assessing their influence on the proliferation of lymphomononuclear cells (LMCs) stimulated with three kinds of mitogens: phorbol 12-myristate 13-acetate (PMA) plus ionomycin, concanavalin A (ConA), and lipopolysaccharide (LPS). Read More

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http://journals.sagepub.com/doi/10.3727/215517916X693159
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http://dx.doi.org/10.3727/215517916X693159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225675PMC
January 2017
49 Reads

Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells.

Cell Med 2017 Jan 1;9(1-2):15-20. Epub 2016 Sep 1.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.

Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. For clinical application of ASCs, serum-free, xeno-free cryopreservation solutions should be used. This study determined the viability and adipo-osteogenic potential of cryopreserved ASCs using four cryopreservation solutions: 10% DMSO, Cell Banker 2 (serum free), Stem Cell Banker (=Cell Banker 3: serum free, xeno free), and TC protector (serum free, xeno free). Read More

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http://dx.doi.org/10.3727/215517916X693122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225674PMC
January 2017
18 Reads

Challenges for Production of Human Transplantable Organ Grafts.

Authors:
Eiji Kobayashi

Cell Med 2017 Jan 21;9(1-2):9-14. Epub 2016 Oct 21.

Department of Organ Fabrication, Keio University School of Medicine , Tokyo , Japan.

The described research methods explain how you could generate a three-dimensional kidney, based on recent research results. The first method is to fabricate human organs in a pig body. The second is to transplant the so-called "organ bud" into a patient's body for further development. Read More

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http://dx.doi.org/10.3727/215517916X693113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225673PMC
January 2017
1 Read

Cell Therapy for Liver Disease Using Bioimaging Rats.

Cell Med 2017 Jan 21;9(1-2):3-7. Epub 2016 Oct 21.

Department of Organ Fabrication, Keio University School of Medicine , Tokyo , Japan.

Advances in stem cell research suggest that cell therapy is a potential alternative to liver transplantation. The use of individualized and minimally invasive cell therapy is desirable to avoid rejection and reduce patient burden. While allo-hepatocyte transplantation has been performed for metabolic hepatic disease, auto-bone marrow transplantation (BMT) has shifted toward mesenchymal stem cells (MSCs) transplantation for liver cirrhosis. Read More

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http://dx.doi.org/10.3727/215517916X693104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225672PMC
January 2017
5 Reads

Facilitating Transplantation.

Authors:
Hirofumi Noguchi

Cell Med 2017 Jan 8;9(1-2):1-2. Epub 2017 Jan 8.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , 207 Uehara, Nishihara, Okinawa 903-0215 , Japan .

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http://dx.doi.org/10.3727/215517916X693078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225671PMC
January 2017
1 Read

Aggregation of Engineered Human β-Cells Into Pseudoislets: Insulin Secretion and Gene Expression Profile in Normoxic and Hypoxic Milieu.

Cell Med 2016 Dec 12;8(3):99-112. Epub 2016 Aug 12.

‡INSERM U1016, Institut Cochin, Paris, France; §Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France.

Innovative treatments to cure type 1 diabetes are being actively researched. Among the different strategies, the replacement of β-cells has given promising results. Classically, islets from cadaveric donors are transplanted into diabetic patients, but recently phase I clinical trials that use stem cell-derived β-cells have been started. Read More

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http://dx.doi.org/10.3727/215517916X692843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5165649PMC
December 2016
12 Reads

Stability Enhancement Using Hyaluronic Acid Gels for Delivery of Human Fetal Progenitor Tenocytes.

Cell Med 2016 Dec 14;8(3):87-97. Epub 2016 Jan 14.

Unit of Regenerative Therapy, Service of Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine, University Hospital of Lausanne , Lausanne , Switzerland.

Tendon afflictions are very common, and their negative impact is high both at the workplace and in leisure activities. Tendinopathies are increasing in prevalence and can lead to tendon ruptures, where healing is a long process with outcomes that are often disappointing. Human fetal progenitor tenocytes (hFPTs) have been recently tested in vitro as a potential cell source to stimulate tendon regeneration. Read More

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http://dx.doi.org/10.3727/215517916X690486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5165648PMC
December 2016
1 Read

αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC Expandability.

Cell Med 2016 Dec 6;8(3):79-85. Epub 2016 Oct 6.

Department of Research, SanBio, Inc., Mountain View, CA, USA; †Clinical Development and Regulatory Affairs, SanBio, Inc., Mountain View, CA, USA.

Clinical applications of mesenchymal stromal cells (MSCs) require the manufacture of large cell lots, which involves multiple passages for cell expansion and sometimes genetic modification. MSCs from various sources, including bone marrow (BM), exhibit high donor-to-donor variability in their growth characteristics. This can lead to unpredictable manufacturing outcomes with respect to success or failure of individual lots. Read More

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http://journals.sagepub.com/doi/10.3727/215517916X693357
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http://dx.doi.org/10.3727/215517916X693357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5165647PMC
December 2016
9 Reads

Allogeneic Mesenchymal Stem Cell Transplantation in Dogs With Keratoconjunctivitis Sicca.

Cell Med 2016 Dec 18;8(3):63-77. Epub 2016 Oct 18.

‡ Instituto Butantan , São Paulo, SP , Brazil.

Keratoconjunctivitis sicca (KCS) is a dysfunction in tear production associated with clinical signs, which include conjunctival hyperemia, ocular discharge, discomfort, pain, and, eventually, corneal vascularization and pigmentation. Immunosuppressive drugs are routinely administrated for long periods to treat KCS but with side effects and limited results. Evaluation of the clinical benefits of intralacrimal transplantation of allogeneic mesenchymal stem cells (MSCs) in dogs with mild-moderate and severe KCS was done. Read More

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http://dx.doi.org/10.3727/215517916X693366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5165646PMC
December 2016
154 Reads

Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application.

Cell Med 2015 Dec 26;8(1-2):57-62. Epub 2015 Aug 26.

Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan; †FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan; ‡Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Japan; §Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-Cho, Takamatsu, Kagawa, Japan.

Quantum dots (QDs) have recently been investigated as fluorescent probes for detecting a very small number of biomolecules and live cells; however, the establishment of molecular imaging technology with on-off control of QD fluorescence remains to be established. Here we have achieved the fluorescence off state of QDs with the conjugation of black hole quencher (BHQ) molecules intermediated with peptide by using streptavidin-QDs585 and biotin-pep-BHQ-1. The fluorescence of streptavidin-QDs585 was decreased by the addition of biotin-pep-BHQ-1 in a dose-dependent manner. Read More

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http://dx.doi.org/10.3727/215517915X689074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733912PMC
December 2015
23 Reads

Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device.

Cell Med 2015 Dec 26;8(1-2):47-56. Epub 2015 Aug 26.

Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine , Tsurumai-cho, Showa-ku, Nagoya , Japan.

In drug discovery, it is very important to evaluate liver cells within an organism. Compared to 2D culture methods, the development of 3D culture techniques for liver cells has been successful in maintaining long-term liver functionality with the formation of a hepatic-specific structure. The key to performing drug testing is the establishment of a stable in vitro evaluation system. Read More

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http://dx.doi.org/10.3727/215517915X689056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733911PMC
December 2015
4 Reads

Human Laminin Isotype Coating for Creating Islet Cell Sheets.

Cell Med 2015 Dec 21;8(1-2):39-46. Epub 2015 Aug 21.

† Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University , Shinjuku-ku, Tokyo , Japan.

Our experimental approach toward the development of new islet-based treatment for diabetes mellitus has been the creation of a monolayered islet cell construct (islet cell sheet), followed by its transplantation into a subcutaneous pocket. Previous studies describe rat laminin-5 (chain composition: α3, β3, γ2) as a suitable extracellular matrix (ECM) for surfaces comprised of a coated temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm). To progress toward the clinical application of this approach, the present study attempted to identify an optimal human ECM as a coating material on PIPAAm surfaces, which allowed islet cells to attach on the surfaces and subsequently to be harvested as a monolithic cell sheet. Read More

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http://dx.doi.org/10.3727/215517915X689029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733910PMC
December 2015
5 Reads

Efficient Gene Transduction of Dispersed Islet Cells in Culture Using Fiber-Modified Adenoviral Vectors.

Cell Med 2015 Dec 26;8(1-2):31-8. Epub 2015 Aug 26.

Department of Regenerative Surgery, Fukushima Medical University , Hikarigaoka, Fukushima , Japan.

To establish novel islet-based therapies, our group has recently developed technologies for creating functional neo-islet tissues in the subcutaneous space by transplanting monolithic sheets of dispersed islet cells (islet cell sheets). Improving cellular function and viability are the next important challenges for enhancing the therapeutic effects. This article describes the adenoviral vector-mediated gene transduction of dispersed islet cells under culture conditions. Read More

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http://dx.doi.org/10.3727/215517915X689083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733909PMC
December 2015
15 Reads

Islet Culture/Preservation Before Islet Transplantation.

Cell Med 2015 Dec 26;8(1-2):25-9. Epub 2015 Aug 26.

# Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.

Although islet culture prior to transplantation provides flexibility for the evaluation of isolated islets and the pretreatment of patients, it is well known that isolated islets deteriorate rapidly in culture. Human serum albumin (HSA) is used for medium supplementation instead of fetal bovine serum (FBS), which is typically used for islet culture research, to avoid the introduction of xenogeneic materials. However, FBS contains several factors that are beneficial to islet viability and which also neutralize the endogenous pancreatic enzymes or exogenous enzymes left over from the isolation process. Read More

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http://dx.doi.org/10.3727/215517915X689047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733908PMC
December 2015
22 Reads

Choice of Feeders Is Important When First Establishing iPSCs Derived From Primarily Cultured Human Deciduous Tooth Dental Pulp Cells.

Cell Med 2015 Dec 26;8(1-2):9-23. Epub 2015 Aug 26.

¶ Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University , Sakuragaoka, Kagoshima , Japan.

Feeder cells are generally required to maintain embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs). Mouse embryonic fibroblasts (MEFs) isolated from fetuses and STO mouse stromal cell line are the most widely used feeder cells. The aim of this study was to determine which cells are suitable for establishing iPSCs from human deciduous tooth dental pulp cells (HDDPCs). Read More

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http://dx.doi.org/10.3727/215517915X689038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733907PMC
December 2015
36 Reads

Cryopreservation of Adipose-Derived Mesenchymal Stem Cells.

Cell Med 2015 Dec 26;8(1-2):3-7. Epub 2015 Aug 26.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.

Mesenchymal stem cells (MSCs) have the potential to differentiate into cells of mesodermal origin such as osteoblasts, adipocytes, myocytes, and chondrocytes. They possess an immunosuppressive effect, which makes them a viable cell population for the cell-based therapy of treatment-resistant immune diseases. Adipose-derived mesenchymal stem cells (ASCs) have been demonstrated to have the ability to acquire the properties of subcutaneous adipose tissue particularly easily, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. Read More

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http://dx.doi.org/10.3727/215517915X689100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733906PMC
December 2015
9 Reads

Creating a Future of Transplantation.

Authors:
Hirofumi Noguchi

Cell Med 2015 Dec 26;8(1-2). Epub 2015 Aug 26.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , 207 Uehara, Nishihara, Okinawa 903-0215 , Japan.

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http://dx.doi.org/10.3727/215517915X689010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733905PMC
December 2015
2 Reads

Disease and Stem Cell-Based Analysis of the 2014 ASNTR Meeting.

Authors:
David J Eve

Cell Med 2015 Oct 8;7(3):133-42. Epub 2015 Jul 8.

Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida Morsani College of Medicine , Tampa, FL , USA.

A wide variety of subjects are presented at the annual American Society of Neural Therapy and Repair meeting every year, as typified by this summary of the 2014 meeting. Parkinson's disease-related presentations were again the most popular topic, with traumatic brain injury, spinal cord injury, and stroke being close behind. Other disorders included Huntington's disease, brain cancer, and bipolar disorders. Read More

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http://dx.doi.org/10.3727/215517915X688606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735576PMC
October 2015
2 Reads

A Combination of Low-Intensity Pulsed Ultrasound and Nanohydroxyapatite Concordantly Enhances Osteogenesis of Adipose-Derived Stem Cells From Buccal Fat Pad.

Cell Med 2015 Oct 22;7(3):123-31. Epub 2015 Apr 22.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University , Tokyo , Japan.

The osteogenic induction of adipose-derived stem cells (ADSCs) has been regarded as an important step in bone tissue engineering. In the present study, we focused on the buccal fat pad (BFP) as a source of adipose tissue, since BFPs are encapsulated by adipose tissue and are often coextirpated during oral surgery. Low-intensity pulsed ultrasound (LIPUS) is effective in the treatment of fractures, and nanohydroxyapatite (NHA) is known as a bone substitute material. Read More

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http://dx.doi.org/10.3727/215517915X688057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735575PMC
October 2015
31 Reads

A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium.

Cell Med 2015 Oct 12;7(3):109-21. Epub 2014 Dec 12.

Department of Biomedical Engineering, Yale University, New Haven, CT, USA; †Department of Anesthesia, Yale University, New Haven, CT, USA.

Respiratory epithelium is difficult to grow in vitro, as it requires a well-maintained polarizing air-liquid interface (ALI) to maintain differentiation. Traditional methods rely on permeable membrane culture inserts, which are difficult to work with and are ill-suited for the production of large numbers of cells, such as the quantities required for cell-based clinical therapies. Herein, we investigate an alternative form of culture in which the cells are placed on a porous substrate that is continuously rolled, such that the monolayer of cells is alternately submerged in media or apically exposed to air. Read More

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http://dx.doi.org/10.3727/215517914X681794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735574PMC
October 2015
36 Reads