232,630 results match your criteria Cell host & microbe[Journal]


A strikingly-angled spindle mediates nuclear migration during colonization of rice cells infected by Magnaporthe oryzae.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Plant Biology, University of Georgia, Athens, GA 30602, USA. Electronic address:

To cause rice blast disease, Magnaporthe oryzae must properly organize microtubules and position nuclei during colonization of host cells. Live cell confocal imaging of fluorescently-tagged microtubules and nuclei of M. oryzae invasive hyphae reveals that microtubules form a cage-like arrangement around nuclei during interphase and that the mitotic spindle forms and mediates nuclear migration while integrity of the nuclear envelope is lost. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.005DOI Listing
February 2019

Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut.

Fish Shellfish Immunol 2019 Feb 19. Epub 2019 Feb 19.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; CAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266400, China. Electronic address:

In order to understand the mediation function of surface proteins in probiotic effects executed by Lactobacillus pentosus HC-2 in midgut of Litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal HC-2 or with stripped surface proteins HC-2 by lithium chloride (LiCl) treatment. The results showed that the shrimp fed with normal HC-2 produced much higher immune and digestion related enzymes than the control group or LiCl-treated HC-2 group to defense the Vibrio parahaemolyticus E1 infection. We obtained total over 275,099 unigenes from L. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.027DOI Listing
February 2019

Risk factors and predictive scoring system for post-transplant lymphoproliferative disorder after hematopoietic stem cell transplantation.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Department of Oncology and Hematology, Shimane University Hospital, Izumo, Japan. Electronic address:

We analyzed data of 64,539 consecutive patients in the national Japanese transplant registry, including 40,195 after allogeneic stem cell transplantation, 24,215 after autologous stem cell transplantation, and 129 after syngeneic stem cell transplantation, and 299 developed Epstein-Barr virus positive post-transplant lymphoproliferative disorder. The probability at 2 years was 0.79% after allogeneic transplantation, 0. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.016DOI Listing
February 2019

Exopolysaccharides synthesized by Bifidobacterium animalis subsp. lactis interact with TLR4 in intestinal epithelial cells.

Anaerobe 2019 Feb 19. Epub 2019 Feb 19.

Microhealth Group, Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias - Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Asturias, Spain. Electronic address:

The toll-like receptors involved in recognition of the exopolysaccharide produced by two isogenic, ropy and non-ropy, Bifidobacterium animalis subsp. lactis strains were investigated. Both strains interact with human embryonic kidney (HEK)-293 cells via TLR2, whereas purified EPSs specifically stimulate TLR4 regardless their molar mass. Read More

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http://dx.doi.org/10.1016/j.anaerobe.2019.02.014DOI Listing
February 2019

Regulation of MicroRNA Machinery and Development by Interspecies S-Nitrosylation.

Cell 2019 Feb;176(5):1014-1025.e12

Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA; Harrington Discovery Institute, University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA. Electronic address:

Bioactive molecules can pass between microbiota and host to influence host cellular functions. However, general principles of interspecies communication have not been discovered. We show here in C. Read More

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http://dx.doi.org/10.1016/j.cell.2019.01.037DOI Listing
February 2019

Structural and functional characterization of the membrane-permeabilizing activity of Nicotiana occidentalis defensin NoD173 and protein engineering to enhance oncolysis.

FASEB J 2019 Feb 22:fj201802540R. Epub 2019 Feb 22.

Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia.

Defensins are an extensive family of host defense peptides found ubiquitously across plant and animal species. In addition to protecting against infection by pathogenic micro-organisms, some defensins are selectively cytotoxic toward tumor cells. As such, defensins have attracted interest as potential antimicrobial and anticancer therapeutics. Read More

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http://dx.doi.org/10.1096/fj.201802540RDOI Listing
February 2019

Genome-wide effect of pulmonary airway epithelial cell-specific Bmal1 deletion.

FASEB J 2019 Feb 22:fj201801682R. Epub 2019 Feb 22.

Centre for Biological Timing, Faculty of Biology, Health and Medicine, University of Manchester, Manchester, United Kingdom.

Pulmonary airway epithelial cells (AECs) form a critical interface between host and environment. We investigated the role of the circadian clock using mice bearing targeted deletion of the circadian gene brain and muscle ARNT-like 1 ( Bmal1) in AECs. Pulmonary neutrophil infiltration, biomechanical function, and responses to influenza infection were all disrupted. Read More

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http://dx.doi.org/10.1096/fj.201801682RDOI Listing
February 2019

Quantitative Proteomic Analysis of A549 Cells Infected with Human Adenovirus Type 2.

J Med Virol 2019 Feb 22. Epub 2019 Feb 22.

Human Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás, Brazil.

HAdV-2 is considered a common agent of respiratory tract infection in the human, especially in children. Virus infection is believed to modify host cell expression necessary for its replication and therefore cell proteome can reflect the changes of specific cellular pathways during infection. This study aims to identify differentially expressed proteins of A549 cells in response to HAdV-2 infection using a label-free liquid chromatography-high-resolution tandem mass spectrometry strategy (LC-MS/MS) at 24 and 48 hpi. Read More

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http://dx.doi.org/10.1002/jmv.25439DOI Listing
February 2019

Microbiome signatures in prostate cancer.

Carcinogenesis 2019 Feb 22. Epub 2019 Feb 22.

Department of Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, Philadelphia, PA, USA.

We have established a microbiome signature for prostate cancer using an array-based metagenomic and capture-sequencing approach. A diverse microbiome signature (viral, bacterial, fungal and parasitic) was observed in the prostate cancer samples compared with benign prostate hyperplasia controls. Hierarchical clustering analysis identified three distinct prostate cancer-specific microbiome signatures. Read More

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http://dx.doi.org/10.1093/carcin/bgz008DOI Listing
February 2019

Dynamic Synthetic Biointerfaces: From Reversible Chemical Interactions to Tunable Biological Effects.

Acc Chem Res 2019 Feb 22. Epub 2019 Feb 22.

Dynamic synthetic biointerface is a new concept of biomaterials with smart surface properties capable of controlled display of bioactive ligands, dynamic modulation of cell-biomaterial interactions, and subsequently clever manipulation of fundamental cell behaviors like adhesion, migration, proliferation, differentiation, apoptosis, and so on. As mimics of the extracellular matrix (ECM), such molecularly dynamic biointerfaces have attracted increasing attention because of their tunable biological effects with great significance in in situ cell biology, tissue engineering, drug targeting, and cell isolation for cancer theranostics. Approaches to control bioligand presentation on materials mainly rely on surface functionalization with dynamic or reversible chemical linkers to which the ligands are tethered. Read More

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http://dx.doi.org/10.1021/acs.accounts.8b00604DOI Listing
February 2019

Comparative analysis of remodelling of the plant-microbe interface in Pisum sativum and Medicago truncatula symbiotic nodules.

Protoplasma 2019 Feb 22. Epub 2019 Feb 22.

All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Laboratory of Molecular and Cellular Biology, Podbelsky chaussee 3, St.-Petersburg, Russia, 196608.

Infection of host cells by nitrogen-fixing soil bacteria, known as rhizobia, involves the progressive remodelling of the plant-microbe interface. This process was examined by using monoclonal antibodies to study the subcellular localisation of pectins and arabinogalactan proteins (AGPs) in wild-type and ineffective nodules of Pisum sativum and Medicago truncatula. The highly methylesterified homogalacturonan (HG), detected by monoclonal antibody JIM7, showed a uniform localisation in the cell wall, regardless of the cell type in nodules of P. Read More

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http://dx.doi.org/10.1007/s00709-019-01355-5DOI Listing
February 2019

Cell Specific Matrix Metalloproteinase-1 Regulates Lung Metastasis Synergistically with Smoke Exposure.

J Cancer Res Forecast 2018 5;1(2). Epub 2018 Nov 5.

Departments of Medicine, Columbia University College of Physicians & Surgeons, New York, NY 10032, USA.

MMP1, a matrix metalloproteinase that degrades the extracellular matrix, is produced not only by cancer cells but also synthesized in stromal and inflammatory cells during tumorigenesis, invasion and lung metastasis. However, the function of MMP1 expression from host cells, especially tumor-associated macrophages (TAMs), and cells in the lung parenchyma remains to be elucidated. Here we demonstrate that macrophages co-cultured with tumor cells drastically enhance MMP1 expression, which is further exacerbated upon cigarette smoke exposure. Read More

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November 2018

Female genital tract chronic graft-versus-host disease: A narrative review.

Hematol Transfus Cell Ther 2019 Jan-Mar;41(1):69-75. Epub 2018 Jul 29.

Fundação Doutor Amaral Carvalho, Jaú, SP, Brazil.

Graft-versus-host disease is one of the main causes of morbidity and mortality in patients submitted to hematopoietic stem cell transplantation. This study reviewed the prevalence of lower female genital tract graft-versus-host disease following hematopoietic stem cell transplantation. A systematic search of the literature for articles published between 1982 and 2015 was performed. Read More

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http://dx.doi.org/10.1016/j.htct.2018.06.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371232PMC

Assessing the impact of ABO incompatibility on major allogeneic hematopoietic stem cell transplant outcomes: a prospective, single-center, cohort study.

Hematol Transfus Cell Ther 2019 Jan-Mar;41(1):1-6. Epub 2018 Jul 10.

Hospital das Clínicas da Universidade Federal de Minas Gerais (HC UFMG), Belo Horizonte, MG, Brazil.

Background: ABO blood group incompatibility between donor and recipient is associated with a number of immunohematological complications, but is not considered a major contraindication to allogeneic hematopoietic stem cell transplantation. However, available evidence from the literature seems to be conflicting as to the impact of incompatibility on overall survival, event-free survival, transplant-related mortality, graft-versus-host disease, and time to neutrophil and platelet engraftment.

Methods: This single-center, prospective, cohort study included patients with hematological malignancies who underwent a first allogeneic hematopoietic stem cell transplantation between 2008 and 2014. Read More

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http://dx.doi.org/10.1016/j.htct.2018.05.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371230PMC

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip.

Front Bioeng Biotechnol 2019 7;7:13. Epub 2019 Feb 7.

Department of Biomedical Engineering, The University of Texas at Austin Austin, TX, United States.

The majority of human gut microbiome is comprised of obligate anaerobic bacteria that exert essential metabolic functions in the human colon. These anaerobic gut bacteria constantly crosstalk with the colonic epithelium in a mucosal anoxic-oxic interface (AOI). However, recreation of the metabolically mismatched colonic AOI has been technically challenging. Read More

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http://dx.doi.org/10.3389/fbioe.2019.00013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374617PMC
February 2019

Phagocytosis of by Human Bronchial Epithelial Cells Is Mediated by the Arp2/3 Complex and WIPF2.

Front Cell Infect Microbiol 2019 7;9:16. Epub 2019 Feb 7.

Centre for Heart Lung Innovation, University of British Columbia and St. Paul's Hospital, Vancouver, BC, Canada.

is an opportunistic fungal pathogen capable of causing severe infection in humans. One of the limitations in our understanding of how causes infection concerns the initial stages of infection, notably the initial interaction between inhaled spores or conidia and the human airway. Using publicly-available datasets, we identified the Arp2/3 complex and the WAS-Interacting Protein Family Member 2 WIPF2 as being potentially responsible for internalization of conidia by airway epithelial cells. Read More

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http://dx.doi.org/10.3389/fcimb.2019.00016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375057PMC
February 2019

Combination of pleuroparenchymal fibroelastosis with non-specific interstitial pneumonia and bronchiolitis obliterans as a complication of hematopoietic stem cell transplantation - Clues to a potential mechanism.

Respir Med Case Rep 2019 4;26:244-247. Epub 2019 Feb 4.

Department of Pathology, Nagasaki University Hospital, Nagasaki, Japan.

Pleuroparenchymal fibroelastosis (PPFE) is a newly described entity of interstitial lung disease, which has been recently recognized as a rare complication of bone marrow transplantation. We report a case of 30-year-old man who developed a unique combination of pleuroparenchymal fibroelastosis with cellular and fibrotic non-specific interstitial pneumonia (NSIP) and bronchiolitis obliterans (BO) sixteen years after hematopoietic stem cell transplantation. Histological examination revealed almost exclusive infiltration of CD3-positive T lymphocytes associated with lymphoepithelial lesions and multi-focal denudation of covering epithelial cells in all components. Read More

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http://dx.doi.org/10.1016/j.rmcr.2019.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370562PMC
February 2019

Evolutionary effects of the AID/APOBEC family of mutagenic enzymes on human gamma-herpesviruses.

Virus Evol 2019 Jan 11;5(1):vey040. Epub 2019 Feb 11.

Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, NY, USA.

The human gamma-herpesviruses, Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus, establish lifelong latency in B cells and are associated with multiple malignancies. Virus-host coevolution often drive changes in both host immunity and in the viral genome. We consider one host immune mechanism, the activation-induced deaminase (AID)/APOBEC family of cytidine deaminases, that induces mutations in viral DNA. Read More

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http://dx.doi.org/10.1093/ve/vey040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371749PMC
January 2019

Fight them or feed them: how the intestinal mucus layer manages the gut microbiota.

Gastroenterol Rep (Oxf) 2019 Feb 13;7(1):3-12. Epub 2019 Feb 13.

Wallenberg Laboratory and Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, Institute of Medicine, Bruna Stråket 16, University of Gothenburg, SE 413 45 Gothenburg, Sweden.

The intestinal tract is inhabited by a tremendous number of microorganisms, termed the gut microbiota. These microorganisms live in a mutualistic relationship with their host and assist in the degradation of complex carbohydrates. Although the gut microbiota is generally considered beneficial, the vast number of microbial cells also form a permanent threat to the host. Read More

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http://dx.doi.org/10.1093/gastro/goy052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375348PMC
February 2019

Interferon-γ Receptor Signaling in Dendritic Cells Restrains Spontaneous Proliferation of CD4 T Cells in Chronic Lymphopenic Mice.

Front Immunol 2019 7;10:140. Epub 2019 Feb 7.

Institute of Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany.

In lymphopenic mice, T cells become activated and undergo lymphopenia-induced proliferation (LIP). However, not all T cells are equally sensitive to lymphopenia. Several lymphopenia-insensitive T cell clones were described and their non-responsiveness was mainly attributed to clone-specific properties. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374634PMC
February 2019

Editorial: Langerhans Cells and How Skin Pathology Reshapes the Local Immune Environment.

Front Immunol 2019 7;10:139. Epub 2019 Feb 7.

Biosciences Department, Durham University, Durham, United Kingdom.

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http://dx.doi.org/10.3389/fimmu.2019.00139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375340PMC
February 2019

Prostaglandins and Other Eicosanoids in Insects: Biosynthesis and Biological Actions.

Front Physiol 2018 7;9:1927. Epub 2019 Feb 7.

Department of Plant Medicals, Andong National University, Andong, South Korea.

This essay reviews the discoveries, synthesis, and biological significance of prostaglandins (PGs) and other eicosanoids in insect biology. It presents the most current - and growing - understanding of the insect mechanism of PG biosynthesis, provides an updated treatment of known insect phospholipase A (PLA), and details contemporary findings on the biological roles of PGs and other eicosanoids in insect physiology, including reproduction, fluid secretion, hormone actions in fat body, immunity and eicosanoid signaling and cross-talk in immunity. It completes the essay with a prospectus meant to illuminate research opportunities for interested readers. Read More

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http://dx.doi.org/10.3389/fphys.2018.01927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375067PMC
February 2019

Orchestration between ILC2s and Th2 cells in shaping type 2 immune responses.

Cell Mol Immunol 2019 Feb 21. Epub 2019 Feb 21.

Molecular and Cellular Immunoregulation Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.

The type 2 immune response is critical for host defense against large parasites such as helminths. On the other hand, dysregulation of the type 2 immune response may cause immunopathological conditions, including asthma, atopic dermatitis, rhinitis, and anaphylaxis. Thus, a balanced type 2 immune response must be achieved to mount effective protection against invading pathogens while avoiding immunopathology. Read More

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http://dx.doi.org/10.1038/s41423-019-0210-8DOI Listing
February 2019

Synthesis and Rational design of Europium and Lithium Doped Sodium Zinc Molybdate with Red Emission for Optical Imaging.

Sci Rep 2019 Feb 21;9(1):2472. Epub 2019 Feb 21.

Department of Physics, Dr. Harisingh Gour Central University, Sagar, 470003, India.

Highly efficient fluorescent and biocompatible europium doped sodium zinc molybdate (NZMOE) nanoprobes were successfully synthesized via Polyol method. Non-radiative defect centres get reduced with Li co-doping in NZMOE nanoprobes. XRD spectra and Rietveld refinement confirmed successful incorporation of lithium ion and crystallinity was also improved with Li co-doping. Read More

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http://dx.doi.org/10.1038/s41598-019-38787-1DOI Listing
February 2019

Mitochondrial localization, import and mitochondrial function of the androgen receptor.

J Biol Chem 2019 Feb 21. Epub 2019 Feb 21.

Genetics, University of Alabama at Birmingham, United States.

Nuclear localization of androgen receptor (AR) directs transcriptional regulation of a host of genes, referred to as genomic signaling. Additionally, non-nuclear or non-genomic activities of the AR have long been described, but the understanding of these activities remains elusive. Here, we report that AR is imported into and localizes to mitochondria and has a novel role in regulating multiple mitochondrial processes. Read More

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http://dx.doi.org/10.1074/jbc.RA118.006727DOI Listing
February 2019

LncRNA SNHG3 promotes cell growth by sponging miR-196a-5p and indicates the poor survival in osteosarcoma.

Int J Immunopathol Pharmacol 2019 Jan-Dec;33:2058738418820743

2 Department of Orthopedic Surgery, Shibei Hospital of Jingan District, Shanghai, China.

Abnormal expression of long noncoding RNAs (lncRNAs) is closely associated with the pathogenesis of multiple malignancies, and lncRNA small nucleolar RNA host genes (SNHGs) play critical roles in tumor progression. However, the mechanism by which SNHG3 contributes to osteosarcoma (OS) remains elusive. The association between SNHG3 expression and the clinicopathological characteristics in OS patients was analyzed using the TCGA (The Cancer Genome Atlas) dataset. Read More

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http://dx.doi.org/10.1177/2058738418820743DOI Listing
February 2019

Identification of Broad-Spectrum Antiviral Compounds by Targeting Viral Entry.

Viruses 2019 Feb 20;11(2). Epub 2019 Feb 20.

MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK.

Viruses are a major threat to human health and economic well-being. In recent years Ebola, Zika, influenza, and chikungunya virus epidemics have raised awareness that infections can spread rapidly before vaccines or specific antagonists can be made available. Broad-spectrum antivirals are drugs with the potential to inhibit infection by viruses from different groups or families, which may be deployed during outbreaks when specific diagnostics, vaccines or directly acting antivirals are not available. Read More

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http://dx.doi.org/10.3390/v11020176DOI Listing
February 2019

Global Epidemiology of Bat Coronaviruses.

Viruses 2019 Feb 20;11(2). Epub 2019 Feb 20.

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.

Bats are a unique group of mammals of the order . They are highly diversified and are the group of mammals with the second largest number of species. Such highly diversified cell types and receptors facilitate them to be potential hosts of a large variety of viruses. Read More

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http://dx.doi.org/10.3390/v11020174DOI Listing
February 2019

Complexities of Type I Interferon Biology: Lessons from LCMV.

Viruses 2019 Feb 20;11(2). Epub 2019 Feb 20.

School of Life and Environmental Sciences, the Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, and the Bosch Institute, The University of Sydney, Sydney, NSW 2006, Australia.

Over the past decades, infection of mice with lymphocytic choriomeningitis virus (LCMV) has provided an invaluable insight into our understanding of immune responses to viruses. In particular, this model has clarified the central roles that type I interferons play in initiating and regulating host responses. The use of different strains of LCMV and routes of infection has allowed us to understand how type I interferons are critical in controlling virus replication and fostering effective antiviral immunity, but also how they promote virus persistence and functional exhaustion of the immune response. Read More

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http://dx.doi.org/10.3390/v11020172DOI Listing
February 2019

Sphingomyelin Depletion from Plasma Membranes of Human Airway Epithelial Cells Completely Abrogates the Deleterious Actions of S. aureus Alpha-Toxin.

Toxins (Basel) 2019 Feb 20;11(2). Epub 2019 Feb 20.

University of Greifswald, Animal Physiology and Biochemistry, Felix Hausdorff-Straße 1, D-17489 Greifswald, Germany.

Interaction of alpha-toxin (hemolysin A, Hla) with eukaryotic cell membranes is mediated by proteinaceous receptors and certain lipid domains in host cell plasma membranes. Hla is secreted as a 33 kDa monomer that forms heptameric transmembrane pores whose action compromises maintenance of cell shape and epithelial tightness. It is not exactly known whether certain membrane lipid domains of host cells facilitate adhesion of Ha monomers, oligomerization, or pore formation. Read More

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http://dx.doi.org/10.3390/toxins11020126DOI Listing
February 2019

A Single-Nucleotide Polymorphism of αβ₃ Integrin Is Associated with the Andes Virus Infection Susceptibility.

Viruses 2019 Feb 20;11(2). Epub 2019 Feb 20.

Departamento de Enfermedades Infecciosas e Inmunologia Pediatricas, División de Pediatría, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago 8330024, Chile.

The (ANDV), which causes the hantavirus cardiopulmonary syndrome, enters cells via integrins, and a change from leucine to proline at residue 33 in the PSI domain (L33P), impairs ANDV recognition. We assessed the association between this human polymorphism and ANDV infection. We defined susceptible and protective genotypes as "TT" (coding leucine) and "CC" (coding proline), respectively. Read More

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http://dx.doi.org/10.3390/v11020169DOI Listing
February 2019

Quality Screening of Incorrectly Folded Soluble Aggregates from Functional Recombinant Proteins.

Int J Mol Sci 2019 Feb 19;20(4). Epub 2019 Feb 19.

Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.

Solubility is the prime criterion for determining the quality of recombinant proteins, yet it often fails to represent functional activity due to the involvement of non-functional, misfolded, soluble aggregates, which compromise the quality of recombinant proteins. However, guidelines for the quality assessment of soluble proteins have neither been proposed nor rigorously validated experimentally. Using the aggregation-prone enhanced green-fluorescent protein (EGFP) folding reporter system, we evaluated the folding status of recombinant proteins by employing the commonly used sonication and mild lysis of recombinant host cells. Read More

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http://dx.doi.org/10.3390/ijms20040907DOI Listing
February 2019

Myeloid Cells during Viral Infections and Inflammation.

Viruses 2019 Feb 19;11(2). Epub 2019 Feb 19.

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Myeloid cells represent a diverse range of innate leukocytes that are crucial for mounting successful immune responses against viruses. These cells are responsible for detecting pathogen-associated molecular patterns, thereby initiating a signaling cascade that results in the production of cytokines such as interferons to mitigate infections. The aim of this review is to outline recent advances in our knowledge of the roles that neutrophils and inflammatory monocytes play in initiating and coordinating host responses against viral infections. Read More

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http://dx.doi.org/10.3390/v11020168DOI Listing
February 2019

Antibacterial Activities of Crude Secondary Metabolite Extracts from Species Obtained from the Stem of and Their Effects on Two Cancer Cell Lines.

Int J Environ Res Public Health 2019 Feb 19;16(4). Epub 2019 Feb 19.

South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Medicine and Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa.

Endophytes are microorganisms that are perceived as non-pathogenic symbionts found inside plants since they cause no symptoms of disease on the host plant. Soil conditions and geography among other factors contribute to the type(s) of endophytes isolated from plants. Our research interest is the antibacterial activity of secondary metabolite crude extracts from the medicinal plant and its bacterial endophytes. Read More

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http://dx.doi.org/10.3390/ijerph16040602DOI Listing
February 2019

Strengthening the AntiTumor NK Cell Function for the Treatment of Ovarian Cancer.

Int J Mol Sci 2019 Feb 19;20(4). Epub 2019 Feb 19.

Department of Experimental Medicine (DIMES) and Centre of Excellence for Biomedical Research (CEBR), University of Genoa, 16132 Genoa, Italy.

The crosstalk between cancer cells and host cells is a crucial prerequisite for tumor growth and progression. The cells from both the innate and adaptive immune systems enter into a perverse relationship with tumor cells to create a tumor-promoting and immunosuppressive tumor microenvironment (TME). Epithelial ovarian cancer (EOC), the most lethal of all gynecological malignancies, is characterized by a unique TME that paves the way to the formation of metastasis and mediates therapy resistance through the deregulation of immune surveillance. Read More

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http://dx.doi.org/10.3390/ijms20040890DOI Listing
February 2019

Biogenesis and secretion of micronemes in Toxoplasma gondii.

Cell Microbiol 2019 Feb 21:e13018. Epub 2019 Feb 21.

Department of Microbiology and Molecular Medicine, University of Geneva CMU, Geneva 4, Switzerland.

One of the hallmarks of the parasitic phylum of Apicomplexa is the presence of highly specialised, apical secretory organelles, called the micronemes and rhoptries that play critical roles in ensuring survival and dissemination. Upon exocytosis, the micronemes release adhesin complexes, perforins and proteases that are crucially implicated in egress from infected cells, gliding motility, migration across biological barriers and host cell invasion. Recent studies on Toxoplasma gondii and Plasmodium species have shed more light on the signalling events and the machinery that trigger microneme secretion. Read More

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http://dx.doi.org/10.1111/cmi.13018DOI Listing
February 2019

Viral CD229 (Ly9) homologs as new manipulators of host immunity.

J Leukoc Biol 2019 Feb 21. Epub 2019 Feb 21.

Immunology Unit, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain.

The signaling lymphocytic activation molecule family (SLAMF) of receptors plays crucial roles during innate and adaptive immune responses. The SLAMF member CD229 (Ly9, SLAMF3) is a homophilic receptor predominantly expressed on the surface of B and T cells. CD229 acts as a cosignaling molecule, regulating lymphocyte homoeostasis and activation. Read More

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http://dx.doi.org/10.1002/JLB.2MR1018-413RDOI Listing
February 2019

The anti-resection activity of the X protein encoded by Hepatitis Virus B.

Hepatology 2019 Feb 21. Epub 2019 Feb 21.

Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), Department of Paediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.

Chronic infection of hepatitis virus B (HBV) is associated with an increased incidence of hepatocellular carcinoma (HCC). HBV encodes an oncoprotein (HBx) that is crucial for viral replication and interferes with multiple cellular activities including gene expression, histone modifications and genomic stability. To date, it remains unclear how disruption of these activities contributes to hepatocarcinogenesis. Read More

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http://dx.doi.org/10.1002/hep.30571DOI Listing
February 2019

Cost-effective production of recombinant peptides in Escherichia coli.

N Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Department of Chemical Sciences, University of Naples Federico II, 80126, Naples, Italy; Istituto Nazionale di Biostrutture e Biosistemi (INBB), Italy. Electronic address:

Among bioactive peptides, cationic antimicrobial peptides (AMPs), also referred to as host defence peptides (HDPs), are valuable tools to treat infections, being able to kill a wide variety of microbes directly and/or modulate host immunity. HDPs have great therapeutic potential against antibiotic-resistant bacteria, viruses and even parasites. However, high manufacturing costs have greatly limited their development as drugs, thus highlighting the need to develop novel and competitive production strategies. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.004DOI Listing
February 2019

Phenotyping of Lewis and secretor HBGA from saliva and detection of new FUT2 gene SNPs from young children from the Amazon presenting acute gastroenteritis and respiratory infection.

Infect Genet Evol 2019 Feb 18. Epub 2019 Feb 18.

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Avenida Brasil, 4365-Manguinhos, Rio de Janeiro, RJ, Brazil.

The Histo-blood group antigens (HBGA) are host genetic factors associated with susceptibility to rotavirus (RV) and human norovirus (HuNoV), the major etiological agents of viral acute gastroenteritis (AGE) worldwide. The FUT2 gene expressing the alpha-1, 2-L- fucosyltransferase enzyme is important for gut HBGA expression, and also provides a composition of the phenotypic profile achieved through mutations occurring in populations with different evolutionary histories; as such, it can be considered a genetic population marker. In this study, Lewis and secretor HBGA phenotyping was performed using 352 saliva samples collected from children between three months and five years old born in the Amazon (Brazil, Venezuela and English Guyana) presenting AGE or acute respiratory infection (ARI), the latter considered as control samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15671348183091
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http://dx.doi.org/10.1016/j.meegid.2019.02.011DOI Listing
February 2019
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Host cell interactome of PB1 N40 protein of H5N1 influenza A virus in chicken cells.

J Proteomics 2019 Feb 18. Epub 2019 Feb 18.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China; State Key Laboratory of Animal Nutrition, Beijing, China; School of Life Science and Engineering, Foshan University, Foshan, China. Electronic address:

H5N1 influenza A virus (IAV) causes seasonal epidemics that represent a worldwide threat to public health. IAV relies on host factors for viral replication. PB1 N40 is translated from the fifth starting code (AUG) of PB1 mRNA, which is the product of the ribosomal scan omission. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.011DOI Listing
February 2019

Baculoviral infection reduces the expression of four allergen proteins of silkworm pupa.

Arch Insect Biochem Physiol 2019 Feb 21:e21539. Epub 2019 Feb 21.

Department of Clinical Veterinary, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.

Silkworm (Bombyx mori) larvae are widely used to express exogenous proteins. Moreover, some silkworm pupal proteins can be used as drug-loading materials for selfexpressed oral tolerance drugs. However, several proteins expressed in silkworm pupae cause severe allergic reactions in humans and animals. Read More

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http://dx.doi.org/10.1002/arch.21539DOI Listing
February 2019

Host factors influence Barrett's carcinogenesis: findings from a mouse gastroduodenal reflux model.

Esophagus 2019 Feb 21. Epub 2019 Feb 21.

Division of Molecular and Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Seta-tsukinowa-cho, Otsu, Shiga, 520-2192, Japan.

Background: Rat gastroduodenal reflux models have been used for analyzing Barrett's carcinogenesis. Mice seem to be more useful than rats for studies targeting genes.

Methods: We induced gastroduodenal contents reflux by esophagojejunostomy using C57BL/6J mice. Read More

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http://dx.doi.org/10.1007/s10388-019-00660-5DOI Listing
February 2019

Role of FcγRs in Antibody-Based Cancer Therapy.

Curr Top Microbiol Immunol 2019 Feb 22. Epub 2019 Feb 22.

Oncology Discovery, Bristol-Myers Squibb, Princeton, NJ, Redwood City, CA, USA.

Monoclonal antibodies can mediate antitumor activity by multiple mechanisms. They can bind directly to tumor receptors resulting in tumor cell death, or can bind to soluble growth factors, angiogenic factors, or their cognate receptors blocking signals required for tumor cell growth or survival. Monoclonal antibodies, upon binding to tumor cell, can also engage the host's immune system to mediate immune-mediated destruction of the tumor. Read More

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http://dx.doi.org/10.1007/82_2019_150DOI Listing
February 2019

Immune Complex Vaccination.

Authors:
Yu-Mei Wen Yan Shi

Curr Top Microbiol Immunol 2019 Feb 22. Epub 2019 Feb 22.

Department of Basic Medical Sciences, Center for Life Sciences, Institute of Immunology, Tsinghua University, Beijing, China.

Antibody/antigen binding results in immune complexes (IC) that have a variety of regulatory functions. One important feature is the enhanced host immune activation against antigen contained in the complex. ICs play important roles at several critical steps that lead to B and T cell activation, including antigen targeting/retention, facilitated antigen uptake, antigen presenting cell activation and proper balancing of positive and negative stimulatory signals. Read More

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http://dx.doi.org/10.1007/82_2019_153DOI Listing
February 2019

The PHO signaling pathway directs lipid remodeling in Cryptococcus neoformans via DGTS synthase to recycle phosphate during phosphate deficiency.

PLoS One 2019 21;14(2):e0212651. Epub 2019 Feb 21.

Centre for Infectious Diseases and Microbiology, Fungal Pathogenesis Group, Westmead Institute for Medical Research, Westmead, New South Wales, Australia.

The phosphate sensing and acquisition (PHO) pathway of Cryptococcus neoformans is essential for growth in phosphate-limiting conditions and for dissemination of infection in a mouse model. Its key transcription factor, Pho4, regulates expression of genes controlling the acquisition of phosphate from both external and cellular sources. One such gene, BTA1, is highly up-regulated during phosphate starvation. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212651PLOS
February 2019

Extracellular vesicles of Trypanosoma cruzi tissue-culture cell-derived trypomastigotes: Induction of physiological changes in non-parasitized culture cells.

PLoS Negl Trop Dis 2019 Feb 21;13(2):e0007163. Epub 2019 Feb 21.

Instituto de Biotecnología, Grupo de Bioquímica y Parasitología Molecular, Departamento de Parasitología, Universidad de Granada, Granada, Spain.

Background: Trypanosoma cruzi is the obligate intracellular parasite that causes Chagas disease. The pathogenesis of this disease is a multifactorial complex process that involves a large number of molecules and particles, including the extracellular vesicles. The presence of EVs of T. Read More

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http://dx.doi.org/10.1371/journal.pntd.0007163DOI Listing
February 2019

Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development.

PLoS Biol 2019 Feb 21;17(2):e3000152. Epub 2019 Feb 21.

Leiden Institute of Physics, Leiden University, Leiden, The Netherlands.

The current understanding of mammalian kidney development is largely based on mouse models. Recent landmark studies revealed pervasive differences in renal embryogenesis between mouse and human. The scarcity of detailed gene expression data in humans therefore hampers a thorough understanding of human kidney development and the possible developmental origin of kidney diseases. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000152DOI Listing
February 2019

Nanopillared Surfaces Disrupt Pseudomonas aeruginosa Mechano-responsive Upstream Motility.

ACS Appl Mater Interfaces 2019 Feb 21. Epub 2019 Feb 21.

Pseudomonas aeruginosa is an opportunistic, multi-drug resistant, human pathogen that forms biofilms in environments with fluid flow, such as the lungs of cystic fibrosis patients, industrial pipelines, and medical devices. P. aeruginosa twitches upstream on surfaces by the cyclic extension and retraction of their mechano-responsive type IV pili motility appendages. Read More

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http://dx.doi.org/10.1021/acsami.8b22262DOI Listing
February 2019

To Explore the Protective Mechanism of PTEN-Induced Kinase 1 (PINK1)/Parkin Mitophagy-Mediated Extract of Periplaneta Americana on Lipopolysaccharide-Induced Cardiomyocyte Injury.

Med Sci Monit 2019 Feb 21;25:1383-1391. Epub 2019 Feb 21.

Department of Geriatrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, China (mainland).

BACKGROUND Sepsis is defined as a systemic inflammatory response syndrome caused by an infection (suspicious or confirmed). Its essence is inflammatory mediators and cytokines mediated by host immune response. The present study aimed to investigate the role of Periplaneta americana extracts (XML) on PTEN-induced kinase 1 (PINK1)/Parkin mediated mitophagy in cardiomyocyte injury by sepsis. Read More

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http://dx.doi.org/10.12659/MSM.912980DOI Listing
February 2019