2,346 results match your criteria Cell biochemistry and biophysics[Journal]


The Role of Arachidonic Acid Metabolism in Myocardial Ischemia-Reperfusion Injury.

Cell Biochem Biophys 2020 Jul 4. Epub 2020 Jul 4.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, PR China.

Patients with myocardial ischemic diseases or who are undergoing one of various heart treatments, such as open heart surgery, coronary artery bypass grafting, percutaneous coronary artery intervention or drug thrombolysis, face myocardial ischemia-reperfusion injury (MIRI). However, no effective treatment is currently available for MIRI. To improve the prognosis of people with cardiovascular disease, it is important to research the mechanism of MIRI. Read More

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http://dx.doi.org/10.1007/s12013-020-00928-zDOI Listing

Hypothetical Pathway for Formation of Cholesterol Microcrystals Initiating the Atherosclerotic Process.

Cell Biochem Biophys 2020 Jun 30. Epub 2020 Jun 30.

Department of Computational Biophysics and Bioinformatics, Jagiellonian University, ul. Gronostajowa 7, 30-387, Krakow, Poland.

Major factors leading to the development of atherosclerosis are a high cholesterol (Chol) level in the blood and oxidative stress. Both promote the formation of Chol microcrystals in blood vessel walls. Deposition of Chol microcrystals in arterial intima causes inflammation, which initiates and accompanies the atherosclerotic process in all its phases. Read More

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http://dx.doi.org/10.1007/s12013-020-00925-2DOI Listing

'Polymorphism-aided' Selective Targeting and Inhibition of Caspase-6 by a Novel Allosteric Inhibitor Towards Efficient Alzheimer's Disease Treatment.

Cell Biochem Biophys 2020 Jun 26. Epub 2020 Jun 26.

Molecular Bio-computation and Drug Design Laboratory, School of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4001, South Africa.

The predominance of Alzheimer's disease (AD) among the aged remains a global challenge. As such, the search for alternative and effective therapeutic options continuous unabated. Among the therapeutic targets explored over the years toward impeding the progression of AD is caspase-6 (Casp6), although selectively targeting Casp6 remains a challenge due to high homology with other members of the caspase family. Read More

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http://dx.doi.org/10.1007/s12013-020-00927-0DOI Listing

Structural and Functional Characterisation of the Domains of Ubiquitin-Activating Enzyme (E1) of Saccharomyces cerevisiae.

Cell Biochem Biophys 2020 Jun 24. Epub 2020 Jun 24.

Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.

Ubiquitin-activating enzyme (E1) is the first enzyme of the ubiquitination pathway and is required to activate ubiquitin. E1 of Saccharomyces cerevisiae is a large multidomain monomeric protein. There are no studies available on the domains of yeast E1 as independent entities. Read More

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http://dx.doi.org/10.1007/s12013-020-00924-3DOI Listing

Prediction of Ubiquitin Ligase Nrdp1-Associated Proteins in Glioma Database.

Cell Biochem Biophys 2020 Jun 19. Epub 2020 Jun 19.

Department of Hematology, Xuzhou Children's Hospital of Xuzhou Medical University, Xuzhou, 221006, Jiangsu, PR China.

The ubiquitin proteasome pathway is conserved from yeast to mammals and is necessary for the targeted degradation of most short-lived proteins in eukaryotic cells. Its protein substrates include cell cycle regulatory proteins and proteins that are not properly folded in the endoplasmic reticulum. Owing to the ubiquity of its protein substrates, ubiquitination regulates a variety of cellular activities, including cell proliferation, apoptosis, autophagy, endocytosis, DNA damage repair, and immune response. Read More

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http://dx.doi.org/10.1007/s12013-020-00926-1DOI Listing

An Update on the Pharmacological Usage of Curcumin: Has it Failed in the Drug Discovery Pipeline?

Cell Biochem Biophys 2020 Jun 5. Epub 2020 Jun 5.

Molecular Bio-computation and Drug Design Laboratory, School of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4001, South Africa.

The pharmacological propensities of curcumin have been reported in a plethora of pre-clinical and clinical studies. However, innate attributes account for extremely low oral bioavailability which impedes its development as a therapeutic agent. Regardless, these drawbacks have not deterred researchers from optimizing its potentials. Read More

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http://dx.doi.org/10.1007/s12013-020-00922-5DOI Listing
June 2020
1.680 Impact Factor

Fumonisin B1 Affects the Biophysical Properties, Migration and Cytoskeletal Structure of Human Umbilical Vein Endothelial Cells.

Cell Biochem Biophys 2020 Jun 5. Epub 2020 Jun 5.

Key Laboratory of Biology and Medical Engineering, Immune Cells and Antibody Engineering Research Center of Guizhou Province, School of Basic Medical Sciences/School of Biology and Engineering, Guizhou Medical University, Guiyang, China.

Fumonisin B1 (FB1) is an important mycotoxin in nature and is a serious threat to human and animal health, but its specific target and molecular mechanism of the toxicity and potential carcinogenicity remain unclear. In this study, we first detected the effects of FB1 on the cell viability, biophysical properties, migration ability, and reactive oxygen species (ROS) of human umbilical vein endothelial cells (HUVECs). Subsequently, changes in the cytoskeletal structure and its binding proteins were analyzed by immunofluorescence and real-time PCR, respectively. Read More

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http://dx.doi.org/10.1007/s12013-020-00923-4DOI Listing

An EPR Study Using Cyclic Hydroxylamines To Assess The Level of Mitochondrial ROS in Superinvasive Cancer Cells.

Cell Biochem Biophys 2020 Jun 1. Epub 2020 Jun 1.

Biomedical Magnetic Resonance, Louvain Drug Research Institute (LDRI), Université catholique de Louvain (UCLouvain), Brussels, Belgium.

It has been proposed that a mitochondrial switch involving a high mitochondrial superoxide production is associated with cancer metastasis. We here report an EPR analysis of ROS production using cyclic hydroxylamines in superinvasive SiHa-F3 compared with less invasive SiHa wild-type human cervix cancer cells. Using the CMH probe, no significant difference was observed in the overall level of ROS between SiHa and SiHa-F3 cells. Read More

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http://dx.doi.org/10.1007/s12013-020-00921-6DOI Listing

C-terminal Tail of β-Tubulin and its Role in the Alterations of Dynein Binding Mode.

Cell Biochem Biophys 2020 May 27. Epub 2020 May 27.

School of Pharmacy, Memorial University of Newfoundland, 300 Prince Philip Dr., St. John's, NL, A1B 3V6, Canada.

Dynein is a cytoskeletal molecular motor protein that moves along the microtubule (MT) and transports various cellular cargos during its movement. Using standard Molecular Dynamics (MD) simulation, Principle Component Analysis (PCA), and Normal Mode Analysis (NMA) methods, this investigation studied large-scale movements and local interactions of dynein's Microtubule Binding Domain (MTBD) when bound to tubulin heterodimer subunits. Examination of the interactions between the MTBD segments, and their adjustments in terms of intra- and intermolecular distances at the interfacial area with tubulin heterodimer, particularly at α-H16, β-H18, and β-tubulin C-terminal tail (CTT), was the main focus of this study. Read More

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http://dx.doi.org/10.1007/s12013-020-00920-7DOI Listing

The influence of iron on selected properties of synthetic pheomelanin.

Cell Biochem Biophys 2020 Jun 24;78(2):181-189. Epub 2020 May 24.

Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

It is believed that while eumelanin plays photoprotective and antioxidant role in pigmented tissues, pheomelanin being more photoreactive could behave as a phototoxic agent. Although the metal ion-sequestering ability of melanin might be protective, transition metal ions present in natural melanins could affect their physicochemical properties. The aim of this research was to study iron binding by pheomelanin and analyze how such a binding affects selected properties of the melanin. Read More

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http://dx.doi.org/10.1007/s12013-020-00918-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266848PMC

XIth EPR Workshop on Applications of EPR in Biology and Medicine.

Cell Biochem Biophys 2020 Jun;78(2):121-122

Medical College of Wisconsin, Milwaukee, WI, USA.

We are pleased and honored to present this special issue for CBBI on the broad topic of biomedical EPR. The papers herein resulted from the most recent October 2019 EPR Workshop in Kraków that encompasses work from outstanding researchers in the field. Before describing the range of articles, we have briefly summarized the history of these workshops and the publications that resulted. Read More

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http://dx.doi.org/10.1007/s12013-020-00916-3DOI Listing

Selective Induction of Cellular Toxicity and Anti-tumor Efficacy by N-Methylpiperazinyl Diarylidenylpiperidone and its Pro-nitroxide Conjugate through ROS-mediated Mitochondrial Dysfunction and G2/M Cell-cycle Arrest in Human Pancreatic Cancer.

Cell Biochem Biophys 2020 Jun 24;78(2):191-202. Epub 2020 May 24.

Departments of Radiology, Geisel School of Medicine, Dartmouth College, 1 Medical Center Drive, Lebanon, NH, 03756, USA.

Pancreatic adenocarcinoma is an aggressive cancer with poor clinical prognosis and limited therapeutic options. There is a significant lack of effective, safe, and targeted therapies for successful treatment of pancreatic cancer. In this report, we describe the anticancer efficacy of two novel compounds, N-methylpiperazinyl diarylidenylpiperidone (L-2663) and its pro-nitroxide conjugate (HO-4589) evaluated on human pancreatic adenocarcinoma (AsPC-1) cell line and xenograft tumor in mice. Read More

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http://dx.doi.org/10.1007/s12013-020-00919-0DOI Listing

Photoprotective Properties of Vitamin D and Lumisterol Hydroxyderivatives.

Cell Biochem Biophys 2020 Jun 22;78(2):165-180. Epub 2020 May 22.

School of Molecular Sciences, The University of Western Australia, Perth, WA, Australia.

We have previously described new pathways of vitamin D3 activation by CYP11A1 to produce a variety of metabolites including 20(OH)D3 and 20,23(OH)D3. These can be further hydroxylated by CYP27B1 to produce their C1α-hydroxyderivatives. CYP11A1 similarly initiates the metabolism of lumisterol (L3) through sequential hydroxylation of the side chain to produce 20(OH)L3, 22(OH)L3, 20,22(OH)L3 and 24(OH)L3. Read More

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http://dx.doi.org/10.1007/s12013-020-00913-6DOI Listing

Effects and Mechanisms of Autophagy Induced by Solubilized-Cholesterol in Hepatocytes: A Comparative Study Among Solvents.

Cell Biochem Biophys 2020 May 21. Epub 2020 May 21.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Cholesterol, the principal sterol in mammalian cells, has been reported to play a role in the pathogenesis of several diseases through autophagy. Due to its insoluble characteristic, all in vitro cholesterol experiments are performed using dimethyl sulphoxide, methyl-β-cyclodextrin, and ethanol co-solvents. To investigate whether the types of solvents have different effects on cholesterol-induced cell behaviors, we analyzed the effects and mechanisms of autophagy induced by solubilized-cholesterol in hepatic cells. Read More

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http://dx.doi.org/10.1007/s12013-020-00917-2DOI Listing

Transcriptomic profiling of long non-coding RNAs in non-virus associated hepatocellular carcinoma.

Cell Biochem Biophys 2020 May 14. Epub 2020 May 14.

Department of Gastroenterology, Center For Digestive Diseases, People's Hospital of Baoan District, The 8th People's Hospital of Shenzhen, Shenzhen, 518101, Guangdong, China.

Due to falling prevalence of viral hepatitis (VH), obesity, alcoholism and related liver diseases have become increasingly frequent and important as causes of hepatocellular carcinoma (HCC). However, mechanisms underlying hepatocarcinogenesis and tumor progression in VH-negative HCC remain poorly understood. Long non-coding RNAs (lncRNAs) have been implicated in pathogenesis of human diseases, including HCC. Read More

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http://dx.doi.org/10.1007/s12013-020-00915-4DOI Listing

A Comparative Study of the Action of Protonophore Uncouplers and Decoupling Agents as Inducers of Free Respiration in Mitochondria in States 3 and 4: Theoretical and Experimental Approaches.

Cell Biochem Biophys 2020 Jun 5;78(2):203-216. Epub 2020 May 5.

Mari State University, pl. Lenina 1, Mari El, Yoshkar-Ola, 424001, Russia.

Theoretical and experimental studies have revealed that that in the liver mitochondria an increase in the rate of free respiration in state 3 induced by protonophore uncouplers 2,4-dinitrophenol and сarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone is equal to or slightly greater than the increase in respiration rate in state 4 induced by these uncouplers. In contrast to these protonophore uncouplers, the decoupler α,ω-tetradecanedioic acid, increasing the rate of respiration in state 4, does not significantly affect the rate of free respiration in state 3. We have proposed quantitative indicators that allow determining the constituent part of the rate of respiration in state 4, associated with the decoupling effect of the uncoupler. Read More

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http://dx.doi.org/10.1007/s12013-020-00914-5DOI Listing

THH Relieves CIA Inflammation by Reducing Inflammatory-related Cytokines.

Cell Biochem Biophys 2020 May 3. Epub 2020 May 3.

Department of Immunology, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.

Tripterygium hypoglaucum hutch (THH) is a plant of the genus tripterygium, which is also known as colquhounia, Gelsemiun elegan, and so on. It is mainly distributed in Yunnan, Guizhou, and Sichuan regions and other places in China. To study the immune mechanism of THH on related inflammatory cytokines in collagen II-induced arthritis (CIA) mice, healthy male C57BL/6 mice were used to model CIA mice. Read More

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http://dx.doi.org/10.1007/s12013-020-00911-8DOI Listing

Identification and Design of a Next-Generation Multi Epitopes Bases Peptide Vaccine Candidate Against Prostate Cancer: An In Silico Approach.

Cell Biochem Biophys 2020 Apr 28. Epub 2020 Apr 28.

Institute for Skeletal Aging & Orthopedic Surgery, Chuncheon Sacred Heart Hospital, Hallym University, Chuncheon, 24252, Republic of Korea.

Prostate cancer (PCa) is the second most diagnosed cancer in men and ranked fifth in overall cancer diagnosis. During the past decades, it has arisen as a significant life-threatening disease in men at an older age. At the early onset of illness when it is in localized form, radiation and surgical treatments are applied against this disease. Read More

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http://dx.doi.org/10.1007/s12013-020-00912-7DOI Listing
April 2020
1.680 Impact Factor

Effect of Electrical Parameters and Cholesterol Concentration on Giant Unilamellar Vesicles Electroformation.

Cell Biochem Biophys 2020 Jun 21;78(2):157-164. Epub 2020 Apr 21.

Department of Medical Physics and Biophysics, University of Split School of Medicine, Split, Croatia.

Giant unilamellar vesicles (GUVs) are used extensively as models that mimic cell membranes. The cholesterol (Chol) content in the fiber cell plasma membranes of the eye lens is extremely high, exceeding the solubility threshold in the lenses of old humans. Thus, a methodological paper pertaining to preparations of model lipid bilayer membranes with high Chol content would significantly help the study of properties of these membranes. Read More

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http://dx.doi.org/10.1007/s12013-020-00910-9DOI Listing

Nitric Oxide Inhibition of Chain Lipid Peroxidation Initiated by Photodynamic Action in Membrane Environments.

Cell Biochem Biophys 2020 Jun 17;78(2):149-156. Epub 2020 Apr 17.

Department of Biophysics, Jagiellonian University, Krakow, Poland.

Iron-catalyzed, free radical-mediated lipid peroxidation may play a major role in tumor cell killing by photodynamic therapy (PDT), particularly when membrane-localizing photosensitizers are employed. Many cancer cells exploit endogenous iNOS-generated NO for pro-survival/expansion purposes and for hyper-resistance to therapeutic modalities, including PDT. In addition to inhibiting the pro-oxidant activity of Fe(II) via nitrosylation, NO may intercept downstream lipid oxyl and peroxyl radicals, thereby acting as a chain-breaking antioxidant. Read More

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http://dx.doi.org/10.1007/s12013-020-00909-2DOI Listing

Solvent Effects on Skin Penetration and Spatial Distribution of the Hydrophilic Nitroxide Spin Probe PCA Investigated by EPR.

Cell Biochem Biophys 2020 Jun 17;78(2):127-137. Epub 2020 Apr 17.

Department of Dermatology, Venereology and Allergology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

Oxidative stress occurs in extrinsic skin aging processes and diseases when the enhanced production of free radicals exceeds the homeostatic antioxidant capacity of the skin. The spin probe, 3-(carboxy)-2,2,5,5-tetramethylpyrrolidin-1-oxyl (PCA), is frequently used to study the cutaneous radical production by electron paramagnetic resonance (EPR) spectroscopy. This approach requires delivering PCA into the skin, yet solvent effects on the skin penetration and spatial distribution of PCA have not been thoroughly investigated. Read More

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http://dx.doi.org/10.1007/s12013-020-00908-3DOI Listing

Retraction Note to: The Association Between Modic Changes of Lumbar Endplates and Spontaneous Absorption of Herniated Intervertebral Discs.

Cell Biochem Biophys 2020 Apr 15. Epub 2020 Apr 15.

Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper. Read More

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http://dx.doi.org/10.1007/s12013-020-00907-4DOI Listing

Effects of Curcumin on Lipid Membranes: an EPR Spin-label Study.

Cell Biochem Biophys 2020 Jun 1;78(2):139-147. Epub 2020 Apr 1.

Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland.

Curcumin is a yellow-orange dye widely used as a spice, food coloring and food preservative. It also exhibits a broad range of therapeutic effects against different disorders such as cancer, diabetes, or neurodegenerative diseases. As a compound insoluble in water curcumin accumulates in cell membranes and due to this location it may indirectly lead to the observed effects by structurally altering the membrane environment. Read More

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http://dx.doi.org/10.1007/s12013-020-00906-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266845PMC

In Silico Prediction of the Effects of Nonsynonymous Single Nucleotide Polymorphisms in the Human Catechol-O-Methyltransferase (COMT) Gene.

Cell Biochem Biophys 2020 Jun 31;78(2):227-239. Epub 2020 Mar 31.

Department of Medical Biochemistry, Faculty of Medicine, Hitit University, Corum, Turkey.

Catechol-O-methyltransferase (COMT) enzyme performs transfer of methyl group to endogenous and exogenous catechol substrates. The COMT enzyme draws interest because of its association with psychiatric, neurological and cardiovascular diseases, and several cancers. Moreover, many prescribed drugs, supplements, and their metabolites are used as substrates of COMT enzyme. Read More

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http://dx.doi.org/10.1007/s12013-020-00905-6DOI Listing

MicroRNA-625-5p Sponges lncRNA MALAT1 to Inhibit Cervical Carcinoma Cell Growth by Suppressing NF-κB Signaling.

Cell Biochem Biophys 2020 Jun 9;78(2):217-225. Epub 2020 Mar 9.

Department of Gynaecology, Zibo Central Hospital, No. 54 Gongqingtuan West Road, Zhangdian District, Zibo, 255036, Shandong, China.

The exact expression profile and potential involvement of miR-625-5p in the tumor biology of cervical carcinoma are still elusive. In this study, we aimed to analyze the expression status and possible involvements of miR-625-5p in both clinical tissue samples and cell culture of cervical carcinoma. The relative expression levels of miR-625-5p and NF-κB transcript were determined by real-time polymerase chain reaction. Read More

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http://dx.doi.org/10.1007/s12013-020-00904-7DOI Listing

Electron Paramagnetic Resonance Imaging of Melanin in Honey Bee.

Cell Biochem Biophys 2020 Jun 21;78(2):123-126. Epub 2020 Feb 21.

Louvain Drug Research Institute, Biomedical Magnetic Resonance Research Group, Université Catholique de Louvain (UCLouvain), Brussels, Belgium.

Honey bees play a crucial role in the nature by pollinating wild flowers. Over the past years, there has been an increasing concern regarding the honey bee colony decline. Pesticides or environmental effects targeting the biochemistry of insect chitin and cuticle coating may be in part responsible for honey bee pathologies. Read More

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http://dx.doi.org/10.1007/s12013-020-00903-8DOI Listing

Biochemical Properties and Effects on Mitochondrial Respiration of Aqueous Extracts of Basidiomycete Mushrooms.

Cell Biochem Biophys 2020 Mar 15;78(1):111-119. Epub 2020 Feb 15.

Department of Biochemistry, State University of Maringá, Maringá, Brazil.

There are different varieties of mushrooms not yet studied spread all over the planet. The objective of this study was to evaluate biochemical properties and effects on mitochondrial respiration of eight Basidiomycete mushrooms: Flaviporus venustus EF30, Hydnopolyporus fimbriatus EF41 and EF44, Inonotus splitgerberi EF46, Oudemansiella canarii EF72, Perenniporia sp. EF79, Phellinus linteus EF81, and Pleurotus albidus EF84. Read More

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http://dx.doi.org/10.1007/s12013-020-00901-wDOI Listing

2020 Letter from the Editor-in-Chief.

Cell Biochem Biophys 2020 03;78(1)

Cell Biochemistry and Biophysics, Denver, CO, USA.

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http://dx.doi.org/10.1007/s12013-020-00902-9DOI Listing

Identification of Differentially Expressed miRNAs in the Response of Spleen CD4 T Cells to Electroacupuncture in Senescence-Accelerated Mice.

Cell Biochem Biophys 2020 Mar 6;78(1):89-100. Epub 2020 Feb 6.

College of Acu-moxibustion and Orthopedics, Hubei University of Chinese Medicine, Wuhan, China.

Immunological aging impairs immune system protection in the body and is associated with high morbidity and mortality in aged people. Electroacupuncture (EA) has been proven to boost immunity. The purpose of this study was to identify the effect of EA on miRNA expression in the immune system of senescence-accelerated mouse P8 (SAMP8) mice. Read More

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http://dx.doi.org/10.1007/s12013-020-00900-xDOI Listing

Electrostatic Tuning of Anion Attraction from the Cytoplasm to the Pore of the CFTR Chloride Channel.

Cell Biochem Biophys 2020 Mar 31;78(1):15-22. Epub 2019 Dec 31.

Department of Physiology and Biophysics, Dalhousie University, PO Box 15000, Halifax, NS, B3H 4R2, Canada.

Anions enter from the cytoplasm into the channel pore of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl channel not via a central pathway but via a single lateral portal or fenestration. High Cl conductance is dependent on electrostatic attraction of cytoplasmic Cl ions by four positively charged amino acid side-chains located within this portal. Here we use a mutagenic approach to investigate the functional effects of transplanting or supplementing these positive charges at nearby portal-lining sites. Read More

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http://dx.doi.org/10.1007/s12013-019-00899-wDOI Listing

Optimization of Ethanol Production using Nitrosative Stress Exposed S.cerevisiae.

Cell Biochem Biophys 2020 Mar 24;78(1):101-110. Epub 2019 Dec 24.

Department of Microbiology, University of North Bengal, Siliguri, India.

S.cerevisiae is an industrially important organism known for its ability to produce ethanol as the demand for ethanol is increasing day by day all over the world, the need to find better and alternative ways to increase ethanol production is also rising. In this work we have proposed such alternative but effective method for producing ethanol by S. Read More

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http://dx.doi.org/10.1007/s12013-019-00897-yDOI Listing

Construction of an Inducible CRISPR/Cas9 System for CXCR4 Gene and Demonstration of its Effects on MKN-45 Cells.

Cell Biochem Biophys 2020 Mar 24;78(1):23-30. Epub 2019 Dec 24.

Department of Anesthesiology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, No. 136, 2nd Zhongshan Rd, Yuzhong District, Chongqing, China.

The CRISPR/Cas9 system is an effective tool for gene editing. However, this conventional expression system cannot control the timing of gene editing and does not utilize resistance screening markers. Therefore, carrying out CRISPR/Cas9 experiments is extremely inconvenient. Read More

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http://dx.doi.org/10.1007/s12013-019-00898-xDOI Listing

Human Rhinovirus Inhibition Through Capsid "Canyon" Perturbation: Structural Insights into The Role of a Novel Benzothiophene Derivative.

Cell Biochem Biophys 2020 Mar 13;78(1):3-13. Epub 2019 Dec 13.

Molecular Bio-computation and Drug Design Laboratory, School of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4001, South Africa.

The challenge in targeting human rhinoviruses (HRV) over the years has been attributed to the wide variety in HRV serotypes. Nonetheless, the search for therapeutic agents against HRV continues unabated. These efforts have been augmented by the recent discovery of the novel benzothiophene derivative shown to inhibit HRV viral replication. Read More

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http://dx.doi.org/10.1007/s12013-019-00896-zDOI Listing
March 2020
1.680 Impact Factor

Construction and Characterization of UBC4 Mutants with Single Residues Swapped from UBC5.

Cell Biochem Biophys 2020 Mar 9;78(1):43-53. Epub 2019 Dec 9.

Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.

Ubiquitination is tightly regulated to control degradation, localization and function of various proteins. Ubiquitination is catalysed by three enzymes, namely E1, E2 and E3. The specificity shown by E2s for E3s holds key to regulation of ubiquitination. Read More

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http://dx.doi.org/10.1007/s12013-019-00894-1DOI Listing

CircMYC Regulates Glycolysis and Cell Proliferation in Melanoma.

Cell Biochem Biophys 2020 Mar 6;78(1):77-88. Epub 2019 Dec 6.

Department of Dermatology, The Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, No. 68 Zhongshan Road, Wuxi, 214002, Jiangsu, China.

Circular RNAs (cicRNAs) have been identified to play pivotal roles in several cancer types. However, functions of circRNA in malignant melanoma are poor defined. Our current study demonstrated that human circMYC was obviously upregulated in human melanoma tissue. Read More

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http://dx.doi.org/10.1007/s12013-019-00895-0DOI Listing

Citral Inhibition of Human Salivary Aldehyde Dehydrogenase.

Cell Biochem Biophys 2020 Mar 15;78(1):31-42. Epub 2019 Nov 15.

Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

Human salivary aldehyde dehydrogenase (hsALDH) protects us from the toxic effect of aldehydes. It has both diagnostic and therapeutic importance. Citral possesses many biological and pharmacological properties. Read More

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http://dx.doi.org/10.1007/s12013-019-00891-4DOI Listing
March 2020
1.680 Impact Factor

Licochalcone H Synthesized by Modifying Structure of Licochalcone C Extracted from Glycyrrhiza inflata Induces Apoptosis of Esophageal Squamous Cell Carcinoma Cells.

Cell Biochem Biophys 2020 Mar 9;78(1):65-76. Epub 2019 Nov 9.

Department of Pharmacy, College of Pharmacy, Mokpo National University, Jeonnam, 58554, Republic of Korea.

Esophageal cancer is one of the malignant cancers with a low 5-year survival rate. Licochalcone (LC) H, a chemically synthesized substance, is a regioisomer of LCC extracted from licorice. The purpose of this study was to determine whether LCH might have anticancer effect on human esophageal squamous cell carcinoma (ESCC) cell lines via apoptosis signaling pathway. Read More

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http://dx.doi.org/10.1007/s12013-019-00892-3DOI Listing

Knockout of the Placenta Specific 8 Gene Affects the Proliferation and Migration of Human Embryonic Kidney 293T Cell.

Cell Biochem Biophys 2020 Mar 6;78(1):55-64. Epub 2019 Nov 6.

Institute for Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, 182 Minyuan Road, Hongshan District, Wuhan, 430074, China.

Candidate oncogene placenta specific 8 (PLAC8) has been identified to participate in different cellular process and human diseases. However, the effects of PLAC8 on cell proliferation and migration in human kidney cancer (KC) remained unclear. In current study, physiological effects of PLAC8 in immortalized human embryonic kidney cell line (HEK293T) were investigated in vitro. Read More

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http://dx.doi.org/10.1007/s12013-019-00893-2DOI Listing
March 2020
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Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro.

Cell Biochem Biophys 2019 Dec 28;77(4):367-377. Epub 2019 Oct 28.

College of Biochemical Engineering, Beijing Union University, Beijing, 100023, China.

Cisplatin is a widely used anti-cancer drug. However, cisplatin is limited in clinical treatment because of its severe nephrotoxicity. This study reported whether O-GSP can antagonize the cisplatin-induced cytotoxicity in HEK293 cells through inducing HO-1 protein expression. Read More

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http://dx.doi.org/10.1007/s12013-019-00890-5DOI Listing
December 2019

Confocal Microscopy Confirmed that in Phosphatidylcholine Giant Unilamellar Vesicles with very High Cholesterol Content Pure Cholesterol Bilayer Domains Form.

Cell Biochem Biophys 2019 Dec 17;77(4):309-317. Epub 2019 Oct 17.

Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, USA.

The cholesterol (Chol) content in the fiber cell plasma membranes of the eye lens is extremely high, exceeding the solubility threshold in the lenses of old humans. This high Chol content forms pure Chol bilayer domains (CBDs) and Chol crystals in model membranes and membranes formed from the total lipid extracts from human lenses. CBDs have been detected using electron paramagnetic resonance (EPR) spin-labeling approaches. Read More

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http://dx.doi.org/10.1007/s12013-019-00889-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858498PMC
December 2019

Tumour Initiation: a Discussion on Evidence for a "Load-Trigger" Mechanism.

Cell Biochem Biophys 2019 Dec 9;77(4):293-308. Epub 2019 Oct 9.

Department of Obstetrics and Gynaecology, University of Otago Christchurch, Christchurch, New Zealand.

Appropriate mechanical forces on cells are vital for normal cell behaviour and this review discusses the possibility that tumour initiation depends partly on the disruption of the normal physical architecture of the extracellular matrix (ECM) around a cell. The alterations that occur thence promote oncogene expression. Some questions, that are not answered with certainty by current consensus mechanisms of tumourigenesis, are elegantly explained by the triggering of tumours being a property of the physical characteristics of the ECM, which is operative following loading of the tumour initiation process with a relevant gene variant. Read More

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http://dx.doi.org/10.1007/s12013-019-00888-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6841748PMC
December 2019
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What is the Mechanism of Nitric Oxide Conversion into Nitrosonium Ions Ensuring S-Nitrosating Processes in Living Organisms.

Authors:
Anatoly F Vanin

Cell Biochem Biophys 2019 Dec 4;77(4):279-292. Epub 2019 Oct 4.

N.N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences; Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Kosygin Str.4, Moscow, 119991, Russia.

Here, I present the data testifying that the conversion of free radical NO molecules to nitrosonium ions (NO), which are necessary for the realization of one of NO biological effects (S-nitrosation), may occur in living organisms after binding NO molecules to loosely bound iron (Fe ions) with the subsequent mutual one-electron oxidation-reduction of NO molecules (their disproportionation). Inclusion of thiol-containing substances as iron ligands into this process prevents hydrolysis of NO ions bound to iron thus providing the formation of stable dinitrosyl iron complexes (DNIC) with thiol ligands. Such complexes act in living organisms as donors of NO and NO, providing stabilization and transfer of these agents via the autocrine and paracrine pathways. Read More

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http://dx.doi.org/10.1007/s12013-019-00886-1DOI Listing
December 2019

Profiles of Physarum Microplasmodial Phosphatase Activity Crucial to Cytoplasmic Streaming and Spherule Formation.

Cell Biochem Biophys 2019 Dec 27;77(4):357-366. Epub 2019 Sep 27.

Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681, Japan.

This study aimed to investigate for the first time, the profile of Physarum microplasmodial phosphatase (PPH) activity toward the phosphorylated light chain of Physarum myosin II (PLCM) at pH 7.6, the velocity of cytoplasmic streaming, and PPH expression in spherule formation during dark starvation (DS). In this study, we cloned the full-length cDNA of PPH using polymerase chain reaction, based on the N-terminal amino acid sequence of the purified enzyme. Read More

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http://dx.doi.org/10.1007/s12013-019-00885-2DOI Listing
December 2019
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Multi-Dimensional Screening Strategy for Drug Repurposing with Statistical Framework-A New Road to Influenza Drug discovery.

Cell Biochem Biophys 2019 Dec 26;77(4):319-333. Epub 2019 Sep 26.

Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632014, India.

Influenza virus is known for its intermittent outbreaks affecting billions of people worldwide. Several neuraminidase inhibitors have been used in practice to overcome this situation. However, advent of new resistant mutants has limited its clinical utilization. Read More

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http://dx.doi.org/10.1007/s12013-019-00887-0DOI Listing
December 2019

Carnosine Prevents Different Structural Damages Induced by Methylglyoxal in Lens Crystallins.

Cell Biochem Biophys 2019 Dec 18;77(4):343-355. Epub 2019 Sep 18.

Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.

The elevated lenticular level of methylglyoxal (MGO) in diabetic patients is an important risk factor for the development of age-related (senile) cataract disorders. Carnosine (β-alanyl L-histidine), a natural antioxidant dipeptide product, has been indicated to prevent the development of cataract diseases. Therefore, it is possible that the anti-cataract activity of this naturally occurring compound is mediated by its anti-glycoxidation effect. Read More

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http://dx.doi.org/10.1007/s12013-019-00884-3DOI Listing
December 2019

Age-Dependent Variation in Glycosylation Features of Alpha-2-Macroglobulin.

Cell Biochem Biophys 2019 Dec 5;77(4):335-342. Epub 2019 Sep 5.

Thrombosis and Atherosclerosis Research Institute, Department of Pediatrics, McMaster University, 237 Barton Street East, Hamilton, ON, L8L 2X2, Canada.

Alpha-2-macroglobulin (A2M) is a glycosylated broad spectrum inhibitor of numerous proteases, including those involved in blood coagulation. Glycosylation characteristics can affect protein structure and function. This study compares glycosylation characteristics of A2M in newborn umbilical cord (NUCP) and adult pooled plasmas. Read More

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http://dx.doi.org/10.1007/s12013-019-00883-4DOI Listing
December 2019

Selective Covalent Inhibition of "Allosteric Cys121" Distort the Binding of PTP1B Enzyme: A Novel Therapeutic Approach for Cancer Treatment.

Cell Biochem Biophys 2019 Sep 24;77(3):203-211. Epub 2019 Aug 24.

Molecular Bio-Computation & Drug Design Lab, School of Health Sciences, University of KwaZulu-Natal, Westville, Durban, 4000, South Africa.

Covalent inhibition targeting noncatalytic residues is rapidly gaining attention in drug discovery. Protein tyrosine phosphatases 1B (PTP1B) is an attractive target for therapeutic interventions in cancer and other diseases. Two binding sites of PTP1B enzyme were identified, catalytic and allosteric. Read More

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http://dx.doi.org/10.1007/s12013-019-00882-5DOI Listing
September 2019

Biocompatibility of Oxygen-Sensing Paramagnetic Implants.

Cell Biochem Biophys 2019 Sep 23;77(3):197-202. Epub 2019 Aug 23.

Department of Radiology, Geisel School of Medicine, Dartmouth College, 1 Medical Center Drive, Lebanon, NH, 03756, USA.

Oxygen-sensing implants, composed of paramagnetic microcrystals embedded in a biocompatible polymer, are increasingly being used for electron paramagnetic resonance (EPR) oximetry in animal models and human subjects. The implants are stable and designed to stay in the tissues for indefinite periods. However, it is not known whether the crystals that may be exposed on the surface of the implants or leached out from the implants will induce cytotoxicity thereby compromising their biocompatibility over the long term. Read More

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http://dx.doi.org/10.1007/s12013-019-00881-6DOI Listing
September 2019
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Merging Preclinical EPR Tomography with other Imaging Techniques.

Cell Biochem Biophys 2019 Sep 22;77(3):187-196. Epub 2019 Aug 22.

Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

This paper presents a survey of electron paramagnetic resonance (EPR) image registration. Image registration is the process of overlaying images (two or more) of the same scene taken at different times, from different viewpoints and/or different techniques. EPR-imaging (EPRI) techniques belong to the functional-imaging modalities and therefore suffer from a lack of anatomical reference which is mandatory in preclinical imaging. Read More

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http://dx.doi.org/10.1007/s12013-019-00880-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6742609PMC
September 2019

Retraction Note to: miR-200 Regulates Epithelial-Mesenchymal Transition in Anaplastic Thyroid Cancer via EGF/EGFR Signaling.

Cell Biochem Biophys 2019 09;77(3):277

Department of Endocrinology, Henan University Huaihe Hospital, Kaifeng, 475000, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper. Read More

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http://dx.doi.org/10.1007/s12013-019-00879-0DOI Listing
September 2019