3,289 results match your criteria Cell Transplantation[Journal]


Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting.

Cell Transplant 2019 Apr 18:963689719844260. Epub 2019 Apr 18.

2 W.M. Keck Center for Collaborative Neuroscience, Rutgers, the State University of New Jersey, Piscataway, New Jersey, USA.

Multilineage-differentiating stress-enduring (Muse) cells are a population of pluripotent stage-specific embryonic antigen 3 (SSEA3)+ mesenchymal stem cells first described by Mari Dezawa in 2010. Although some investigators have reported SSEA3+ mesenchymal cells in umbilical cord tissues, none have quantitatively compared SSEA3+ cells isolated from Wharton's jelly (WJ) and the cord lining (CL) of human umbilical cords (HUCs). We separated WJ and the CL from HUCs, cultured mesenchymal stromal cells (MSCs) isolated from these two tissues with collagenase, and quantified the percentage of SSEA3+ cells over three passages. Read More

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http://dx.doi.org/10.1177/0963689719844260DOI Listing

The Preconditioning of Busulfan Promotes Efficiency of Human CD133+ Cells Engraftment in NOD Shi-SCID IL2Rγcnull (NOG) Mice via Intra-Bone Marrow Injection.

Cell Transplant 2019 Apr 15:963689719842162. Epub 2019 Apr 15.

2 Department of Clinical Immunology, School of Laboratory Medicine, Xinxiang Medical University, China.

Human CD133 stem cells were injected into the bone marrow cavity of NOG (NOD Shi-SCID IL2Rγc) mice with or without preconditioning of busulfan in order to assess the efficiency of human CD133 cells engraftment. Peripheral blood from CD133-engrafted NOG mice was analyzed by flow cytometry. The results showed that human CD19 B lymphocytes could be detected at 4 weeks post-transplantation, and human CD4, CD8 subsets of T lymphocytes, CD19 CD14 HLA-DR DCs and CD19 CD14 monocytes could be detected at 16 weeks post-transplantation. Read More

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http://dx.doi.org/10.1177/0963689719842162DOI Listing

Plaque Distribution of Basilar Artery Fenestration by 3D High-Resolution MR Vessel Wall Imaging.

Cell Transplant 2019 Apr 15:963689719843813. Epub 2019 Apr 15.

2 Department of Radiology, China-Japan Friendship Hospital, Beijing, China.

The association between fenestrations and neurovascular pathology is not well defined. The morphology of vessel wall plays an important role in the development of neurovascular pathology. We sought to explore the plaque distribution around basilar artery fenestration (BAF) by three-dimensional high-resolution MR vessel wall imaging (3D HRMRI). Read More

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http://dx.doi.org/10.1177/0963689719843813DOI Listing
April 2019
1 Read

The Effect of Enzymatic Digestion on Cultured Epithelial Autografts.

Cell Transplant 2019 Apr 15:963689719833305. Epub 2019 Apr 15.

2 Department of Hand Surgery, Plastic Surgery and Burns, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.

Severe burns are often treated by means of autologous skin grafts, preferably following early excision of the burnt tissue. In the case of, for example, a large surface trauma, autologous skin cells can be expanded in vitro prior to transplantation to facilitate the treatment when insufficient uninjured skin is a limitation. In this study we have analyzed the impact of the enzyme (trypsin or accutase) used for cell dissociation and the incubation time on cell viability and expansion potential, as well as expression of cell surface markers indicative of stemness. Read More

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http://dx.doi.org/10.1177/0963689719833305DOI Listing

MicroRNA-133a and Myocardial Infarction.

Cell Transplant 2019 Apr 14:963689719843806. Epub 2019 Apr 14.

1 Department of Cardiology, Xiangya Hospital, Central South University, Changsha, PR China.

Myocardial infarction (MI) is the leading cause of morbidity and mortality in the world. The infarcted heart displays typical cell death cascades characterized by a loss of cells and fibrotic scarring in the myocardium. Cardiac hypertrophy and fibrosis largely contribute to ventricular wall thickening and stiffening, altogether defining an adverse cardiac remodeling that ultimately leads to impaired cardiac function and subsequent heart failure. Read More

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http://dx.doi.org/10.1177/0963689719843806DOI Listing

Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells.

Cell Transplant 2019 Apr 14:963689719842166. Epub 2019 Apr 14.

1 Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

We have previously reported human fetal cartilage progenitor cells (hFCPCs) as a novel source of therapeutic cells showing high proliferation and stem cell properties superior to those of adult mesenchymal stem cells (MSCs). In this study, we investigated the immunophenotype and immune-modulatory activities of hFCPCs. With institutional review board approval, hFCPCs were isolated from fetuses at 11-13 weeks of gestation. Read More

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http://dx.doi.org/10.1177/0963689719842166DOI Listing

Potential Roles of NIX/BNIP3L Pathway in Rat Traumatic Brain Injury.

Cell Transplant 2019 Apr 8:963689719840353. Epub 2019 Apr 8.

5 Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, SuZhou, China.

NIX/BNIP3L is known as a proapoptotic protein that is also related to mitophagy. Previous reports have shown that NIX could be involved in neuronal apoptosis after intracerebral hemorrhage, but it also plays a protective role in mitophagy in ischemic brain injury. How NIX works in traumatic brain injury (TBI) is unclear. Read More

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http://dx.doi.org/10.1177/0963689719840353DOI Listing
April 2019
1 Read

CDNF Protein Therapy in Parkinson's Disease.

Cell Transplant 2019 Apr 4:963689719840290. Epub 2019 Apr 4.

3 Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.

Neurotrophic factors (NTF) are a subgroup of growth factors that promote survival and differentiation of neurons. Due to their neuroprotective and neurorestorative properties, their therapeutic potential has been tested in various neurodegenerative diseases. Bioavailability of NTFs in the target tissue remains a major challenge for NTF-based therapies. Read More

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http://dx.doi.org/10.1177/0963689719840290DOI Listing
April 2019
2 Reads

Silencing of the Mutant Huntingtin Gene through CRISPR-Cas9 Improves the Mitochondrial Biomarkers in an In Vitro Model of Huntington's Disease.

Cell Transplant 2019 Apr 4:963689719840662. Epub 2019 Apr 4.

1 Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, USA.

During the 25-year history of the American Society for Neural Therapy and Repair (ASNTR) there have been several breakthroughs in the area of neurotherapeutics, which was the case during the 2014-2105 year when one of us (GLD) had the privilege of serving as its president. During that year, the use of a newly developed gene-editing tool, the CRISPR-Cas9 system, started to skyrocket. Although scientists unraveled the use of "clustered regularly interspaced short palindromic repeats" (CRISPR) and its associated genes from the Cas family as an evolved mechanism of some bacterial and archaeal genomes to protect themselves from being hijacked by invasive viral genes, its use as a therapeutic tool was not fully appreciated until further research revealed how this system operated and how it might be developed technologically to manipulate genes of any species. Read More

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http://dx.doi.org/10.1177/0963689719840662DOI Listing
April 2019
2 Reads

Circular RNAs: Diversity of Functions and a Regulatory Nova in Oral Medicine: A Pilot Review.

Cell Transplant 2019 Apr 4:963689719837917. Epub 2019 Apr 4.

1 Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, People's Republic of China.

Oral diseases, such as cancers, inflammation, loss of bone/tooth/soft tissues, are serious threats to human health since some can cause systemic disease and effective treatments are limited. Thus, discovering promising biomarkers for physiological and pathological processes in oral medicine, and identifying novel targets for therapy have become a most critical issue. Recently, circular RNAs (circRNAs), which were once thought to be a class of non-coding RNAs (ncRNAs), are found to be of coding potential. Read More

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http://dx.doi.org/10.1177/0963689719837917DOI Listing

Association between Non-Alcoholic Fatty Liver Disease and Intracerebral Hemorrhage.

Cell Transplant 2019 Mar 29:963689719840025. Epub 2019 Mar 29.

1 Department of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, China.

Objective:: To determine whether non-alcoholic fatty liver disease (NAFLD) and intracerebral hemorrhage (ICH) are connected, and assess the role played by NAFLD in ICH development.

Methods:: A retrospective study evaluated inpatients treated at the First Affiliated Hospital of Zhejiang University. We divided the patients into Group A (ICH with NAFLD) and Group B (ICH alone). Read More

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http://dx.doi.org/10.1177/0963689719840025DOI Listing
March 2019
1 Read
3.570 Impact Factor

Long-Term Outcomes of BMMSC Compared with BMMNC for Treatment of Critical Limb Ischemia and Foot Ulcer in Patients with Diabetes.

Cell Transplant 2019 Mar 27:963689719835177. Epub 2019 Mar 27.

1 Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China.

Background:: We first compared long-term clinical outcomes in treating critical limb ischemia (CLI) and foot ulcer in patients with diabetes between autologous bone marrow mesenchymal stem cell (BMMSC) and bone-marrow-derived mononuclear cell (BMMNC) transplants. Methods: Forty-one patients were enrolled and followed up for 3 years. They received an 18-day standard treatment before stem cell transplantation. Read More

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http://dx.doi.org/10.1177/0963689719835177DOI Listing
March 2019
1 Read
3.570 Impact Factor

Optimized Generation of Primary Human Epithelial Cells from Larynx and Hypopharynx: A Site-Specific Epithelial Model for Reflux Research.

Cell Transplant 2019 Mar 27:963689719838478. Epub 2019 Mar 27.

1 Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Laryngopharyngeal reflux (LPR) induces a differential damage effect on several anatomic sites within the larynx and hypopharynx; therefore, an in vitro model is needed for each anatomic site. This study aimed to establish a primary culture method for human laryngeal and hypopharyngeal epithelial cells derived from multiple anatomic sites. Surgical mucosa specimens were treated with a two-step enzymatic strategy to establish a primary culture. Read More

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http://dx.doi.org/10.1177/0963689719838478DOI Listing

In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation.

Cell Transplant 2019 Mar 27:963689719825614. Epub 2019 Mar 27.

1 Schepens Eye Research Institute of Massachusetts Eye and Ear, Boston, MA, USA.

One of the current limitations of retinal transplantation of stem cells as well as other cell types is the dispersion of cells from the injection site (including loss of cells into the vitreous chamber) and low survival after transplantation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) conjugate is a biodegradable polymer that can undergo covalent cross-linking in situ, allowing for injection of incorporated cells through a small caliber needle followed by gel formation in vivo. We tested the hypothesis that Gtn-HPA hydrogel supports survival and integration of retinal progenitor cells (RPCs) post-transplantation. Read More

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http://dx.doi.org/10.1177/0963689719825614DOI Listing

Evaluation of Diffusional Kurtosis Imaging in Sub-acute Ischemic Stroke: Comparison with Rehabilitation Treatment Effect.

Cell Transplant 2019 Mar 25:963689719837919. Epub 2019 Mar 25.

1 Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.

Stroke is a serious worldwide medical condition that causes neurological function disability. Diffusional kurtosis imaging, which measures the non-Gaussianity of water diffusion, has been demonstrated to be a sensitive biomarker in many neuro-pathologies. This study explores the relationship between neural function recovery and transformation of the ischemic lesion and/or corticospinal tract during the sub-acute phase after stroke by using diffusional kurtosis imaging. Read More

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http://dx.doi.org/10.1177/0963689719837919DOI Listing
March 2019
1 Read

α-Synuclein Expression Is Preserved in Substantia Nigra GABAergic Fibers of Young and Aged Neurotoxin-Treated Rhesus Monkeys.

Cell Transplant 2019 Mar 11:963689719835794. Epub 2019 Mar 11.

1 Neuroscience Training Program, University of Wisconsin-Madison, USA.

α-Synuclein (α-syn) is a small presynaptic protein distributed ubiquitously in the central and peripheral nervous system. In normal conditions, α-syn is found in soluble form, while in Parkinson's Disease (PD) it may phosphorylate, aggregate, and combine with other proteins to form Lewy bodies. The purpose of this study was to evaluate, in nonhuman primates, whether α-syn expression is affected by age and neurotoxin challenge. Read More

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http://dx.doi.org/10.1177/0963689719835794DOI Listing

The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation.

Cell Transplant 2019 Mar 6:963689719833303. Epub 2019 Mar 6.

1 Department of Pathophysiology, Jagiellonian University Medical College, Krakow, Poland.

The autonomic innervation of the uterus is involved in multiple pathophysiological processes in both humans and animals. Pathological conditions such as adenomyosis or inflammatory pelvic disease are usually accompanied by significant alterations in uterine innervation. In the current study, we focused on autonomic innervation of uterine fibroids, the identification of recently described interstitial cells, telocytes, and the possible interplay between these structures. Read More

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http://dx.doi.org/10.1177/0963689719833303DOI Listing
March 2019
5 Reads
3.570 Impact Factor

Characterization of CDNF-Secreting ARPE-19 Cell Clones for Encapsulated Cell Therapy.

Cell Transplant 2019 Mar 6:963689719827943. Epub 2019 Mar 6.

1 Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.

Cerebral Dopamine Neurotrophic Factor (CDNF) shows beneficial effects in rodent models of Parkinson's and Alzheimer's disease. The brain is a challenging target for protein therapy due to its exclusive blood-brain barrier. Hence, the therapeutic protein should be delivered directly to the brain parenchyma. Read More

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http://dx.doi.org/10.1177/0963689719827943DOI Listing

Sodium/Hydrogen Exchanger 1 Participates in Early Brain Injury after Subarachnoid Hemorrhage both in vivo and in vitro via Promoting Neuronal Apoptosis.

Cell Transplant 2019 Mar 6:963689719834873. Epub 2019 Mar 6.

1 Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

Sodium/hydrogen exchanger 1 (NHE1) plays an essential role in maintaining intracellular pH (pHi) homeostasis in the central nervous system (CNS) under physiological conditions, and it is also associated with neuronal death and intracellular Na and Ca overload induced by cerebral ischemia. However, its roles and underlying mechanisms in early brain injury (EBI) induced by subarachnoid hemorrhage (SAH) have not been fully explored. In this research, a SAH model in adult male rat was established through injecting autologous arterial blood into prechiasmatic cistern. Read More

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http://dx.doi.org/10.1177/0963689719834873DOI Listing
March 2019
2 Reads

Lycium barbarum (Wolfberry) Increases Retinal Ganglion Cell Survival and Affects both Microglia/Macrophage Polarization and Autophagy after Rat Partial Optic Nerve Transection.

Cell Transplant 2019 Mar 6:963689719835181. Epub 2019 Mar 6.

2 Collaborative Joint Laboratory, Guangdong-Hongkong-Macau Institute of CNS Regeneration, Ministry of Education CNS Regeneration, Jinan University, Guangzhou, China.

The rat partial optic nerve transection (PONT) model has been used for studying secondary degeneration of retinal ganglion cells (RGCs) in recent years. In this study, we carried out PONT of the temporal side of rat optic nerves, whereas PONT was carried out of the superior side in the previous publication. We found that this surgery is better and easier than the previous method and can produce a repeatable and reliable model. Read More

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http://dx.doi.org/10.1177/0963689719835181DOI Listing
March 2019
7 Reads

Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation and Differentiation after Intracerebral Hemorrhage in Mice.

Cell Transplant 2019 Mar 4:963689719834870. Epub 2019 Mar 4.

1 Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China.

Repetitive transcranial magnetic stimulation (rTMS) is a physical treatment applied during recovery after intracerebral hemorrhage (ICH). With in vivo and in vitro assays, the present study sought to investigate how rTMS influences neural stem cells (NSCs) after ICH and the possible mechanism. Following a collagenase-induced ICH, adult male C57BL/6 J mice were subjected to rTMS treatment every 24 h for 5 days using the following parameters: frequency, 10 Hz; duration, 2 s; wait time, 5. Read More

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http://dx.doi.org/10.1177/0963689719834870DOI Listing
March 2019
2 Reads

Iron Dyshomeostasis Induces Binding of APP to BACE1 for Amyloid Pathology, and Impairs APP/Fpn1 Complex in Microglia: Implication in Pathogenesis of Cerebral Microbleeds.

Cell Transplant 2019 Feb 18:963689719831707. Epub 2019 Feb 18.

1 Department of Neurology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

As a putative marker of cerebral small vessel disease, cerebral microbleeds (CMBs) have been associated with vascular cognitive impairment. Both iron accumulation and amyloid protein precursor (APP) dysregulation are recognized as pathological hallmarks underlying the progression of CMBs, but their cross-talk is not yet understood. In this study, we found a profound increase of amyloid formation with increasing FeCl treatment, and a distinct change in APP metabolism and expression of iron homeostasis proteins (ferritin, Fpn1, iron regulatory protein) was observed at the 300 uM concentration of FeCl. Read More

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http://dx.doi.org/10.1177/0963689719831707DOI Listing
February 2019
1 Read

Novel Insights into MSK1 Phosphorylation by MRKβ in Intracerebral Hemorrhage-Induced Neuronal Apoptosis.

Cell Transplant 2019 Feb 11:963689719829073. Epub 2019 Feb 11.

1 Department of Neurosurgery, Comprehensive Surgical Laboratory, Affiliated Hospital of Nantong University, P.R. China.

Neuronal apoptosis is regarded as one of the most important pathophysiological changes of intracerebral hemorrhagic (ICH) stroke-a major public health problem that leads to high mortality rates and functional dependency. Mitogen-and stress-activated kinase (MSK) 1 is implicated in various biological functions in different cell types, including proliferation, tumorigenesis and responses to stress. Our previous study showed that MSK1 phosphorylation (p-MSK1) is related to the regulation of LPS-induced astrocytic inflammation, and possibly acts as a negative regulator of inflammation. Read More

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http://dx.doi.org/10.1177/0963689719829073DOI Listing
February 2019

Construction of Pedicled Smooth Muscle Tissues by Combining the Capsule Tissue and Cell Sheet Engineering.

Cell Transplant 2019 Mar 9;28(3):328-342. Epub 2019 Jan 9.

1 Department of Urology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

The survival of engineered tissue requires the formation of its own capillary network, which can anastomose with the host vasculature after transplantation. Currently, while many strategies, such as modifying the scaffold material, adding endothelial cells, or angiogenic factors, have been researched, engineered tissue implanted in vivo cannot timely access to sufficient blood supply, leading to ischemic apoptosis or shrinkage. Constructing vascularized engineered tissue with its own axial vessels and subsequent pedicled transfer is promising to solve the problem of vascularization in tissue engineering. Read More

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http://dx.doi.org/10.1177/0963689718821682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425107PMC

Targeting MicroRNA-21 Suppresses Gastric Cancer Cell Proliferation and Migration via PTEN/Akt Signaling Axis.

Cell Transplant 2019 Mar 30;28(3):306-317. Epub 2019 Jan 30.

1 Department of Gastroenterology, Jinshan Hospital of Fudan University, Shanghai, China.

MicroRNA plays a pivotal role in various human cancers, especially in human gastric cancer. In the present study, we evaluated the effect of microRNA-21 (miR-21) on the gastric cancer cell proliferation, migration, apoptosis and the related signaling cascades. Here, we showed that down-regulation of miR-21 markedly reduced gastric cancer cell proliferation (AGS and NCI-N87 cells) in a time dependent manner. Read More

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http://dx.doi.org/10.1177/0963689719825573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425105PMC
March 2019
1 Read
3.570 Impact Factor

Intravesical Administration of Xenogeneic Porcine Urothelial Cells Attenuates Cyclophosphamide-Induced Cystitis in Mice.

Cell Transplant 2019 Mar 24;28(3):296-305. Epub 2019 Jan 24.

1 Departments of Medical Laboratory Science and Biotechnology and Urology, Sex Hormone Research Center, China Medical University and Hospital, Taichung.

The urothelium of the bladder, renal pelvis, ureter and urethra is maintained through the regulated proliferation and differentiation of urothelial stem and progenitor cells. These cells provide a rich source of a novel urothelial cell therapy approach that could be used to protect, regenerate, repair and restore a damaged urothelium. Urothelial injury caused by physical, chemical and microbial stress is the pathological basis of cystitis (bladder inflammation). Read More

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http://journals.sagepub.com/doi/10.1177/0963689718822773
Publisher Site
http://dx.doi.org/10.1177/0963689718822773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425110PMC
March 2019
8 Reads

Steroid-Free Immune Suppression Impairs Glycemic Control in a Healthy Cynomolgus Monkey.

Cell Transplant 2019 Mar 24;28(3):262-268. Epub 2019 Jan 24.

1 Diabetes Research Institute, Miller School of Medicine, University of Miami, FL, USA.

The need for chronic immune suppression (IS) is one of the hurdles precluding widespread use of islet cell transplantation to restore glycemic control in patients with type 1 diabetes. We report the case of a healthy nonhuman primate (NHP) treated on and off for over 2.5 years with steroid-free IS, consisting of daclizumab induction and maintenance therapy with rapamycin and low dose tacrolimus. Read More

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http://dx.doi.org/10.1177/0963689718823505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425109PMC
March 2019
1 Read

Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro.

Cell Transplant 2019 Mar 24;28(3):286-295. Epub 2019 Jan 24.

4 Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning, China.

Augmenting the biological function of adipose-derived stromal cells (ASCs) is a promising approach to promoting tissue remodeling in regenerative medicine. Here, we examined the effect of ginsenoside Rg1 on the paracrine activity and adipogenic differentiation capacity of human breast ASCs (hbASCs) in vitro. hbASCs were isolated and characterized in terms of stromal cell surface markers and multipotency. Read More

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http://dx.doi.org/10.1177/0963689719825615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425106PMC
March 2019
9 Reads

Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin (DADLE) Exerts a Cytoprotective Effect in Astrocytes Exposed to Oxygen-Glucose Deprivation by Inducing Autophagy.

Cell Transplant 2019 Jan 22:963689719825619. Epub 2019 Jan 22.

1 Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, China.

Astrocytes protection and functional regulation are important strategies to protect against neuronal damage caused by ischemia. Activation of the delta opioid receptor (DOR) could reduce astrocytes damage, although the mechanism remains unclear. The present study aimed to test the effect of DOR activation on autophagy in astrocytes exposed to oxygen-glucose deprivation (OGD), and to further investigate whether this effect has a protective effect on astrocytes. Read More

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http://dx.doi.org/10.1177/0963689719825619DOI Listing
January 2019

Phenothiazines Enhance the Hypothermic Preservation of Liver Grafts: A Pilot in Vitro Study.

Cell Transplant 2019 Mar 22;28(3):318-327. Epub 2019 Jan 22.

3 Department of Neurosurgery, Wayne State University School of Medicine, Detroit, MI, USA.

In vitro liver conservation is an issue of ongoing critical importance in graft transplantation. In this study, we investigated the possibility of augmenting the standard pre-transplant liver conservation protocol (University of Wisconsin (UW) cold solution) with the phenothiazines chlorpromazine and promethazine. Livers from male Sprague-Dawley rats were preserved either in UW solution alone, or in UW solution plus either 2. Read More

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http://dx.doi.org/10.1177/0963689718824559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425111PMC

Pathophysiology of Ganglioside GM1 in Ischemic Stroke: Ganglioside GM1: A Critical Review.

Cell Transplant 2019 Jan 22:963689718822782. Epub 2019 Jan 22.

4 Department of Physiology & Pharmacology, Loma Linda University School of Medicine, USA.

Ganglioside GM1 is a member of the ganglioside family which has been used in many countries and is thought of as a promising alternative treatment for preventing several neurological diseases, including cerebral ischemic injury. The therapeutic effects of GM1 have been proved both in neonates and in adults following ischemic brain damage; however, its clinical efficacy in patients with ischemic stroke is still uncertain. This review examines the recent knowledge of the neuroprotective properties of GM1 in ischemic stroke, collected in the past two decades. Read More

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http://dx.doi.org/10.1177/0963689718822782DOI Listing
January 2019
3.570 Impact Factor

Bridging Therapy Versus Direct Mechanical Thrombectomy in Patients with Acute Ischemic Stroke due to Middle Cerebral Artery Occlusion: A Clinical-Histological Analysis of Retrieved Thrombi.

Cell Transplant 2019 Jan 18:963689718823206. Epub 2019 Jan 18.

1 Department of Neurology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients with middle cerebral artery (MCA) occlusion remains unclear. Therefore, this study aimed to compare the effects of direct MT and bridging therapy, and to elucidate the correlation between thrombus composition and stroke subtypes. Read More

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http://dx.doi.org/10.1177/0963689718823206DOI Listing
January 2019
3 Reads

The Effect of iPS-Derived Neural Progenitors Seeded on Laminin-Coated pHEMA-MOETACl Hydrogel with Dual Porosity in a Rat Model of Chronic Spinal Cord Injury.

Cell Transplant 2019 Jan 18:963689718823705. Epub 2019 Jan 18.

1 Department of Tissue Culture and Stem Cells, Institute of Experimental Medicine, CAS, Prague, Czech Republic.

Spinal cord injury (SCI), is a devastating condition leading to the loss of locomotor and sensory function below the injured segment. Despite some progress in acute SCI treatment using stem cells and biomaterials, chronic SCI remains to be addressed. We have assessed the use of laminin-coated hydrogel with dual porosity, seeded with induced pluripotent stem cell-derived neural progenitors (iPSC-NPs), in a rat model of chronic SCI. Read More

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http://dx.doi.org/10.1177/0963689718823705DOI Listing
January 2019
4 Reads

Transplanting Cells for Spinal Cord Repair: Who, What, When, Where and Why?

Cell Transplant 2019 Jan 18:963689718824097. Epub 2019 Jan 18.

1 Department of Neurobiology and Anatomy, College of Medicine, Drexel University, Philadelphia, PA, USA.

Cellular transplantation for repair of the injured spinal cord has a rich history with strategies focused on neuroprotection, immunomodulation, and neural reconstruction. The goal of the present review is to provide a concise overview and discussion of five key themes that have become important considerations for rebuilding functional neural networks. The questions raised include: (i) who are the donor cells selected for transplantation, (ii) what is the intended target for repair, (iii) when is the optimal time for transplantation, (iv) where should the cells be delivered, and lastly (v) why does cell transplantation remain an attractive candidate for promoting neural repair after injury? Recent developments in neurobiology and engineering now enable us to start addressing these questions with multidisciplinary expertise and methods. Read More

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http://dx.doi.org/10.1177/0963689718824097DOI Listing
January 2019
1 Read

Treatment Strategy for Unruptured Intracranial Aneurysm in Elderly Patients: Coiling, Clipping, or Conservative?

Cell Transplant 2019 Jan 16:963689718823517. Epub 2019 Jan 16.

1 Division of Cerebrovascular Surgery and Interventional Neuroradiology, Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.

An increasing number of unruptured intracranial aneurysms (UIAs) has been discovered in elderly patients in recent years, but the optimal treatment strategy for these patients remains controversial. We report our six-year experience treating UIAs in elderly patients (≥ 70 years old). A retrospective review was conducted of elderly patients who harbored UIAs treated by conservative observation, microsurgical clipping, or endovascular coiling between January 2009 and December 2014. Read More

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http://dx.doi.org/10.1177/0963689718823517DOI Listing
January 2019
9 Reads

The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study.

Cell Transplant 2019 Jan 14:963689718814470. Epub 2019 Jan 14.

1 IRCCS Centro Neurolesi "Bonino-Pulejo", Messina, Italy.

Mesenchymal stem cells (MSCs) are widely used in stem cell therapy for regenerative purposes. Oral-derived MSCs, such as gingival MSCs (GMSCs), deriving from the neural crest seem more suitable to differentiate toward the neuronal lineage. In addition, the preconditioning of MSCs may improve their beneficial effects. Read More

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http://dx.doi.org/10.1177/0963689718814470DOI Listing
January 2019
1 Read

Administration of rCTRP9 Attenuates Neuronal Apoptosis Through AdipoR1/PI3K/Akt Signaling Pathway after ICH in Mice.

Cell Transplant 2019 Jan 14:963689718822809. Epub 2019 Jan 14.

2 Department of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA, USA.

Targeting neuronal apoptosis after intracerebral hemorrhage (ICH) may be an important therapeutic strategy for ICH patients. Emerging evidence indicates that C1q/TNF-Related Protein 9 (CTRP9), a newly discovered adiponectin receptor agonist, exerts neuroprotection in cerebrovascular disease. The aim of this study was to investigate the anti-apoptotic role of CTRP9 after experimental ICH and to explore the underlying molecular mechanisms. Read More

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http://dx.doi.org/10.1177/0963689718822809DOI Listing
January 2019
3 Reads

MRI Tracking of iPS Cells-Induced Neural Stem Cells in Traumatic Brain Injury Rats.

Cell Transplant 2018 Dec 21:963689718819994. Epub 2018 Dec 21.

2 Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.

Induced pluripotent stem cells (iPS cells) are promising cell source for stem cell replacement strategy applied to brain injury caused by traumatic brain injury (TBI) or stroke. Neural stem cell (NSCs) derived from iPS cells could aid the reconstruction of brain tissue and the restoration of brain function. However, tracing the fate of iPS cells in the host brain is still a challenge. Read More

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http://dx.doi.org/10.1177/0963689718819994DOI Listing
December 2018
6 Reads

Transplantation of Human Neural Progenitor Cells (NPC) into Putamina of Parkinsonian Patients: A Case Series Study, Safety and Efficacy Four Years after Surgery.

Cell Transplant 2019 Mar 21;28(3):269-285. Epub 2018 Dec 21.

9 Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.

Individuals with Parkinson's disease (PD) suffer from motor and mental disturbances due to degeneration of dopaminergic and non-dopaminergic neuronal systems. Although they provide temporary symptom relief, current treatments fail to control motor and non-motor alterations or to arrest disease progression. Aiming to explore safety and possible motor and neuropsychological benefits of a novel strategy to improve the PD condition, a case series study was designed for brain grafting of human neural progenitor cells (NPCs) to a group of eight patients with moderate PD. Read More

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http://dx.doi.org/10.1177/0963689718820271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425108PMC
March 2019
1 Read

Therapeutic Target and Cell-signal Communication of Chlorpromazine and Promethazine in Attenuating Blood-Brain Barrier Disruption after Ischemic Stroke.

Cell Transplant 2019 Feb 20;28(2):145-156. Epub 2018 Dec 20.

1 China-America Institute of Neuroscience, Luhe Hospital, Capital Medical University, Beijing, China.

Ischemic stroke destroys blood-brain barrier (BBB) integrity. There are currently no effective treatments available in the clinical setting. Post-ischemia treatment with phenothiazine drugs [combined chlorpromazine and promethazine (C+P)] has been shown to be neuroprotective in stroke. Read More

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http://dx.doi.org/10.1177/0963689718819443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362522PMC
February 2019
2 Reads

Progress and Updates in Stroke Research: Introduction to the Special Issue on Stroke.

Cell Transplant 2018 Dec;27(12):1709-1710

6 Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida Morsani College of Medicine, Tampa, FL, USA.

As guest editors of this special issue, we are delighted to present 13 articles that discuss advances in stroke research including translational stroke research, stroke research milestones, and proposals for future directions. Stroke is a major cause of disability, and, according to the Centers for Disease Control and Prevention, is the fifth leading cause of death in the US alone. Possible factors contributing to stroke pathogenesis are elucidated and possible treatment modalities are explored in the following special issue of Cell Transplantation. Read More

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http://dx.doi.org/10.1177/0963689718816102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300769PMC
December 2018
1 Read
3.570 Impact Factor

Bone Mesenchymal Stem Cell-Enriched β-Tricalcium Phosphate Scaffold Processed by the Screen-Enrich-Combine Circulating System Promotes Regeneration of Diaphyseal Bone Non-Union.

Cell Transplant 2019 Feb 17;28(2):212-223. Epub 2018 Dec 17.

1 Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shang Hai, P.R. China.

Bone non-union after fracture, considered a therapeutic challenge for orthopedics, always needs a reversion surgery, including autograft transplantation (AGT). However, adverse events related to autograft harvest cannot be ignored. Our group designed a novel system called the bone marrow stem cell Screen-Enrich-Combine Circulating System (SECCS) by seeding mesenchymal stem cells (MSCs) into β-tricalcium phosphate (β-TCP) during surgery to thereafter rapidly process bioactive bone implantation. Read More

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http://dx.doi.org/10.1177/0963689718818096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362520PMC
February 2019
4 Reads

The Diagnostic Utility of p16 Immunostaining in Differentiating Cancer and HSIL from LSIL and Benign in Cervical Cells.

Cell Transplant 2019 Feb 14;28(2):195-200. Epub 2018 Dec 14.

4 Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China.

Cervical liquid-based cytology plays an important role in the diagnosis of cervical squamous intraepithelial lesion (SIL). However, cytological evaluation alone has a relatively low sensitive. To overcome this problem, HPV DNA testing or HPV DNA combined with cytology has been applied. Read More

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http://dx.doi.org/10.1177/0963689718817478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362523PMC
February 2019
2 Reads

The Efficacy of Near-Infrared Spectroscopy Monitoring in Carotid Endarterectomy: A Prospective, Single-Center, Observational Study.

Cell Transplant 2019 Feb 14;28(2):170-175. Epub 2018 Dec 14.

1 Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing, China.

There has been no gold standard for intraoperative monitoring in carotid endarterectomy (CEA) till now. The purpose of the current study was to investigate the value of near-infrared spectroscopy (NIRS) monitoring in CEA and explore the thresholds for intraoperative cerebral hypoperfusion. Eighty-four consecutive patients who underwent CEA surgery in Xuan Wu Hospital of Capital Medical University from August 2015 to June 2016 were enrolled in this study. Read More

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http://dx.doi.org/10.1177/0963689718817760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362521PMC
February 2019
4 Reads
3.570 Impact Factor

Age-Appropriateness of Porcine Models Used for Cell Transplantation.

Cell Transplant 2019 Feb 10;28(2):224-228. Epub 2018 Dec 10.

1 Department of Organ Fabrication, Keio University School of Medicine, Tokyo, Japan.

Pigs have traditionally been used for preclinical experiments, and body size-matching is important for cell therapy in animal models used for preclinical trials. It has been shown that the efficacy of the transplanted cells is dependent on the response of the host heart and the age of experimental pigs. Read More

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http://dx.doi.org/10.1177/0963689718817477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362526PMC
February 2019
1 Read

Potential of Exosomes for the Treatment of Stroke.

Cell Transplant 2018 Dec 6:963689718816990. Epub 2018 Dec 6.

1 Department of Anatomy, Histology and Embryology, Discipline of Neuroscience, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Stroke is the result of blockage or rupture of blood vessels in the brain and is the leading cause of death and disability in the world. Currently only a very limited number of therapeutic approaches are available for treatment of stroke patients, and the vast majority of neuroprotective agents that tested positively in pre-clinical studies failed in clinical trials. In recent years, the clinical value of the use of exosomes for stroke treatment has received widespread attention due their unique characteristics such as low immunogenicity, low toxicity and biodegradability, ability to cross the blood-brain barrier (BBB), and their important role in communication between cells. Read More

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http://dx.doi.org/10.1177/0963689718816990DOI Listing
December 2018
33 Reads

Validation of a New North American Islet Donor Score for Donor Pancreas Selection and Successful Islet Isolation in a Medium-Volume Islet Transplant Center.

Cell Transplant 2019 Feb 6;28(2):185-194. Epub 2018 Dec 6.

1 Department of Surgery, University of Chicago, Chicago, IL, USA.

The selection of optimal pancreas donors is one of the key factors in determining the ultimate outcome of clinical islet isolation. North American Islet Donor Score (NAIDS) allows for estimating the chance of the success of islet isolation. It was developed based on the data from over 1000 donors from 11 islet isolation centers and validated in the University of Alberta, Edmonton, on the cohort from the most active islet transplant center. Read More

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http://dx.doi.org/10.1177/0963689718816989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362524PMC
February 2019
13 Reads
3.570 Impact Factor

Size, Aspect Ratio and Anatomic Location of Ruptured Intracranial Aneurysms: Consecutive Series of 415 Patients from a Prospective, Multicenter, Observational Study.

Cell Transplant 2018 Dec 4:963689718817227. Epub 2018 Dec 4.

1 Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

To analyze the size and location distribution of ruptured intracranial aneurysms (IAs) helps to provide evidence for clinical treatment of unruptured IAs using this feature of aneurysms. In this study, 415 patients who presented with an acute subarachnoid hemorrhage caused by IAs were enrolled from eight tertiary referral centers between June 2016 and March 2018. The size, aspect ratio and anatomic location of ruptured IAs were defined and reported by patient sex. Read More

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http://dx.doi.org/10.1177/0963689718817227DOI Listing
December 2018
4 Reads

Detrimental Role of miRNA-144-3p in Intracerebral Hemorrhage Induced Secondary Brain Injury is Mediated by Formyl Peptide Receptor 2 Downregulation Both In Vivo and In Vitro.

Cell Transplant 2018 Dec 4:963689718817219. Epub 2018 Dec 4.

1 Department of Neurosurgery, Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.

Although microRNA-144-3p (miRNA-144-3p) has been shown to suppress tumor proliferation and invasion, its function in intracerebral hemorrhage (ICH)-induced secondary brain injury (SBI) remains unclear. Thus, this study was designed to investigate the role of miRNA-144-3p in ICH. To accomplish this, we used adult male Sprague-Dawley rats to establish an in vivo ICH model by injecting autologous blood, while cultured primary rat cortical neurons were exposed to oxyhemoglobin (OxyHb) to mimic ICH in vitro. Read More

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http://dx.doi.org/10.1177/0963689718817219DOI Listing
December 2018
14 Reads

Transplantation of Amniotic Fluid-Derived Stem Cells Preconditioned with Glial Cell Line-Derived Neurotrophic Factor Gene Alleviates Renal Fibrosis.

Cell Transplant 2019 Jan 30;28(1):65-78. Epub 2018 Nov 30.

1 Department of Nephrology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Amniotic fluid-derived stem cells (AFSCs), which exhibit both embryonic and mesenchymal stem cell characteristics, have been shown to mitigate the degree of renal interstitial fibrosis. The aim of the present study was to determine whether transplantation of glial cell line-derived neurotrophic factor (GDNF)-modified AFSCs is more useful than transplantation of unmodified AFSCs for the treatment of renal interstitial fibrosis. Mice were randomly assigned to a sham-operation group (sham), a unilateral ureteral obstruction (UUO)-saline solution group (UUO), an AFSC transplantation group (AFSC) and a GDNF-modified AFSC transplantation group (GDNF-AFSC) and sacrificed at days 3 and 7 post-surgery (six in each group). Read More

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http://dx.doi.org/10.1177/0963689718815850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322139PMC
January 2019
6 Reads
3.570 Impact Factor