3,452 results match your criteria Cell Transplantation[Journal]


Enhanced Bone Formation in Osteoporotic Mice by a Novel Transplant Combined with Adipose-derived Stem Cells and Platelet-rich Fibrin Releasates.

Cell Transplant 2020 Jan-Dec;29:963689720927398

School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.

Osteoporotic fracture is the main complication of osteoporosis (OP) and accounts for millions of injuries annually. Local intervention by intra-marrow injection has been a good option for preventing osteoporotic bone loss when the osteoporotic femoral fracture has been treated. In this study, tail vein transplantations were examined to evaluate the cell-based therapeutic approach for treating OP with adipose-derived stem cells (ADSCs) and platelet-rich fibrin releasates (PRFr) in an ovariectomized (OVX) mice model. Read More

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http://dx.doi.org/10.1177/0963689720927398DOI Listing

LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2.

Cell Transplant 2020 Jan-Dec;29:963689720925059

Department of Pediatrics, Taihe Hospital, Hubei University of Medicine, Shiyan City, China.

Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an "oncogene," regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism of FEZF1-AS1 on gastric cancer stem cell (GCSC) is yet to be investigated. Read More

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http://dx.doi.org/10.1177/0963689720925059DOI Listing

Umbilical Cord Blood Units Cryopreserved in the Public Cord Blood Bank: A Breakthrough in iPSC Haplobanking?

Cell Transplant 2020 Jan-Dec;29:963689720926151

Department of Laboratory Medicine, Seoul National University Boramae Medical Center, Republic of Korea.

The use of induced pluripotent stem cells (iPSCs) is an emerging therapeutic option for precision medicine. Cord blood (CB) cells with lower immunogenicity, fewer genomic changes, and persistent epigenetic memory might be ideal candidates for iPSC production. Based on the human leukocyte antigen (HLA) distribution of cord blood units (CBUs) in the public CB bank, we estimated the coverage of the Korean population with HLA-homozygous iPSCs to repurpose cryopreserved CBUs. Read More

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http://dx.doi.org/10.1177/0963689720926151DOI Listing

Linc02381 Exacerbates Rheumatoid Arthritis Through Adsorbing miR-590-5p and Activating the Mitogen-Activated Protein Kinase Signaling Pathway in Rheumatoid arthritis-fibroblast-like synoviocytes.

Authors:
Jing Wang Qing Zhao

Cell Transplant 2020 Jan-Dec;29:963689720938023

Department of Rheumatology and Immunology, Huaihe Hospital of Henan University, Kaifeng, People's Republic of China.

Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease. New evidence suggested that linc02381 suppressed colorectal cancer progression by regulating PI3 K signaling pathway, but the role of linc02381 in other diseases, such as RA, remains unclear. This study aimed to reveal the mechanism of linc02381 in RA progression. Read More

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http://dx.doi.org/10.1177/0963689720938023DOI Listing

Intra-articular Injection of Cell-laden 3D Microcryogels Empower Low-dose Cell Therapy for Osteoarthritis in a Rat Model.

Cell Transplant 2020 Jan-Dec;29:963689720932142

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.

Intra-articular injection of mesenchymal stem cells (MSCs) in an osteoarthritic joint can help slow down cartilage destruction. However, cell survival and the efficiency of repair are generally low due to mechanical damage during injection and a high rate of cell loss. We, thus, investigated an improved strategy for cell delivery to an osteoarthritic joint through the use of three-dimensional (3D) microcryogels. Read More

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http://dx.doi.org/10.1177/0963689720932142DOI Listing

Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.

Cell Transplant 2020 Jan-Dec;29:963689720927392

Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.

In vivo beta-cell neogenesis may be one way to treat diabetes. We aimed to investigate the effect of glucagon-like peptide-1 (GLP-1) on beta-cell neogenesis in type 2 diabetes mellitus (T2DM). Male C57BL/6J mice, 6 wk old, were randomly divided into three groups: Control, T2DM, and T2DM + Lira. Read More

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http://dx.doi.org/10.1177/0963689720927392DOI Listing

Cell-Free DNA Variant Sequencing Using CTC-Depleted Blood for Comprehensive Liquid Biopsy Testing in Metastatic Breast Cancer.

Cell Transplant 2020 Jan-Dec;29:963689720925057

Istituto Scientifico Romagnolo per Lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.

Keup and colleagues provide liquid biopsy preliminary results by sequencing variants in circulating tumor cells (CTCs) and cell-free deoxyribonucleic acid (cfDNA) "all from one tube" format, in order to use the same blood sample under the same isolation conditions of both analytes to reach an unbiased comparability and consistency. We appreciated the attempt of the authors to improve technical procedures in liquid biopsy research area, but we wanted to raise several issues related to cfDNA detection, reporting our research experience. This is a feasibility study as the authors analyzed only one sample from a small case series at an advanced line of treatment. Read More

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http://dx.doi.org/10.1177/0963689720925057DOI Listing

miR-26b-5p/TCF-4 Controls the Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells.

Cell Transplant 2020 Jan-Dec;29:963689720934418

Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, PR China.

In this study, we assessed the ability of miR-26b-5p to regulate T cell factor 4 (TCF-4) expression and thereby control human adipose-derived mesenchymal stem cell (hADMSC) adipogenic differentiation. Adipogenic medium was used to induce hADMSC differentiation over a 6-d period. The ability of miR-26b-5p to interact with the TCF-4 mRNA was confirmed through both predictive bioinformatics analyses and luciferase reporter assays. Read More

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http://dx.doi.org/10.1177/0963689720934418DOI Listing

The Role of Interleukin-1β in Destruction of Transplanted Islets.

Cell Transplant 2020 Jan-Dec;29:963689720934413

Cell Transplantation and Gene Therapy Institute, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.

Islet transplantation is a promising β-cell replacement therapy for type 1 diabetes, which can reduce glucose lability and hypoglycemic episodes compared with standard insulin therapy. Despite the tremendous progress made in this field, challenges remain in terms of long-term successful transplant outcomes. The insulin independence rate remains low after islet transplantation from one donor pancreas. Read More

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http://dx.doi.org/10.1177/0963689720934413DOI Listing

Regenerative Potential of Platelet-Rich Fibrin Releasate Combined with Adipose Tissue-Derived Stem Cells in a Rat Sciatic Nerve Injury Model.

Cell Transplant 2020 Jan-Dec;29:963689720919438

Bioinnovation Center, Tzu Chi Foundation, Hualien, Taiwan.

Sciatic nerve injuries, not uncommon in trauma with a limited degree of functional recovery, are considered a persistent clinical, social, and economic problem worldwide. Accumulating evidence suggests that stem cells can promote the tissue regeneration through various mechanisms. The aim of the present study was to investigate the role of adipose tissue-derived stem cells (ADSCs) and combine with platelet-rich fibrin releasate (PRFr) in the regeneration of sciatic nerve injury in rats. Read More

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http://dx.doi.org/10.1177/0963689720919438DOI Listing

A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood.

Cell Transplant 2020 Jan-Dec;29:963689720923574

Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan.

Peripheral blood (PB) contains several types of stem/progenitor cells, including hematopoietic stem and endothelial progenitor cells. We identified a population positive for both the pluripotent surface marker SSEA-3 and leukocyte common antigen CD45 that comprises 0.04% ± 0. Read More

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http://dx.doi.org/10.1177/0963689720923574DOI Listing

Comparison of Allogeneic Platelet-rich Plasma With Autologous Platelet-rich Plasma for the Treatment of Diabetic Lower Extremity Ulcers.

Cell Transplant 2020 Jan-Dec;29:963689720931428

Department of Endocrinology and Nephrology, Chongqing University Central Hospital, Chongqing Emergency Medical Center, China.

Registration Number Of Clinical Trials: ChiCTR1900021317. Read More

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http://dx.doi.org/10.1177/0963689720931428DOI Listing
June 2020
3.570 Impact Factor

DNA Methylation-based Diagnostic and Prognostic Biomarkers for Glioblastoma.

Cell Transplant 2020 Jan-Dec;29:963689720933241

Department of Critical Care Medicine, The First Affiliated Hospital of Nanchang University, Jiangxi, China.

Glioblastomas are the most common primary central nervous system malignancy tumor in adults. Glioblastoma patients have poor prognosis, with an average survival period of approximately 14 mo after diagnosis. To date, there are a limited number of effective treatment methods for glioblastoma, and its molecular mechanisms remain elusive. Read More

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http://dx.doi.org/10.1177/0963689720933241DOI Listing

A Limited Sampling Strategy for Therapeutic Drug Monitoring of Mycophenolate Mofetil for Prophylaxis of Acute Graft-Versus-Host Disease in Allogeneic Stem Cell Transplantation.

Cell Transplant 2020 Jan-Dec;29:963689720912925

Homi Bhabha National Institute, Mumbai, Maharastra, India.

A universally accepted strategy for therapeutic drug monitoring (TDM) of mycophenolate mofetil (MMF) in the prevention of acute graft-versus-host disease (aGVHD) in allogenic hematopoietic stem cell transplantation (alloHSCT) does not exist. We explored the feasibility of developing a limited sampling strategy (LSS) for TDM of MMF in this setting. Patients undergoing alloHSCT received standard MMF-cyclosporine prophylaxis, with MMF administered twice daily (BD) for matched transplant recipients or thrice daily (TID) in haploidentical transplantation. Read More

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http://dx.doi.org/10.1177/0963689720912925DOI Listing

Polysaccharide Enzymatic Hydrolysate Suppresses the Growth of Human Colon Cancer Cells via Inducing Apoptosis.

Cell Transplant 2020 Jan-Dec;29:963689720931435

Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.

is a popular traditional Chinese medicine used in China to improve health. Previous researches have revealed that the polysaccharide from could exert diversity activities, including immunomodulation, antioxidant, and antitumor effects. However, the effect of enzymatically hydrolyzed polysaccharide (EGLP) in colorectal cancer (CRC) progression remains unknown. Read More

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http://dx.doi.org/10.1177/0963689720931435DOI Listing

Long Noncoding RNA LINC00460 Promotes Cell Progression by Sponging miR-4443 in Head and Neck Squamous Cell Carcinoma.

Cell Transplant 2020 Jan-Dec;29:963689720927405

Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Nanjing Medical University, Jiangsu, China.

Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers worldwide. Long noncoding RNAs were proved to be associated with the development and progression in HNSCC. However, the mechanism of LINC00460 in HNSCC needs to be further investigated. Read More

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http://dx.doi.org/10.1177/0963689720927405DOI Listing
June 2020
3.570 Impact Factor

Resveratrol Ameliorates the Malignant Progression of Pancreatic Cancer by Inhibiting Hypoxia-induced Pancreatic Stellate Cell Activation.

Cell Transplant 2020 Jan-Dec;29:963689720929987

Department of Geriatric Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Pancreatic cancer is characterized by a hypoxic tumor microenvironment, which is primarily caused by massive fibrosis with pancreatic stellate cells (PSCs) as a main component. Our previous studies have shown that resveratrol can significantly inhibit pancreatic cancer. However, whether resveratrol can inhibit hypoxia-induced cancer development remains unclear. Read More

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http://dx.doi.org/10.1177/0963689720929987DOI Listing
May 2020
3.570 Impact Factor

Amniotic Fluid Stem Cells Improve Rat Bladder Dysfunction After Pelvic Nerve Transection.

Cell Transplant 2020 Jan-Dec;29:963689720909387

College of Medicine, Chang Gung University, Taoyuan.

The effects of human amniotic fluid stem cells (hAFSCs) transplantation on bladder dysfunction after pelvic nerve transection (PNT) remain to be clarified. Five groups of female Sprague-Dawley rats were studied including sham operation, unilateral PNT alone or plus hAFSCs transplantation, and bilateral PNT alone or plus hAFSCs transplantation. hAFSCs were injected at the site of PNT. Read More

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http://dx.doi.org/10.1177/0963689720909387DOI Listing

SDF-1/CXCR4 Augments the Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells in the Treatment of Lipopolysaccharide-Induced Liver Injury by Promoting Their Migration Through PI3K/Akt Signaling Pathway.

Cell Transplant 2020 Jan-Dec;29:963689720929992

Department of Intensive Care Unit, The Second People's Hospital of Yunnan Province (The Fourth Affiliated Hospital of Kunming Medical University), Kunming, Yunnan Province, China.

Mesenchymal stem cells (MSCs) are thought to have great potential in the therapy of acute liver injury. It is possible that these cells may be regulated by the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor-4 (CXCR4) signaling axis, which has been shown to promote stem cells migration in the inflammation-associated diseases. However, the effects of SDF-1/CXCR4 axis on the MSCs-transplantation-based treatment for acute liver injury and the underlying mechanisms are largely unknown. Read More

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http://dx.doi.org/10.1177/0963689720929992DOI Listing

Data Mining Identifies Six Proteins that Can Act as Prognostic Markers for Head and Neck Squamous Cell Carcinoma.

Cell Transplant 2020 Jan-Dec;29:963689720929308

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract affecting the oral cavity, lips, paranasal sinuses, larynx, and nasopharynx. Proteogenomics combines proteomics and genomics and employs mass spectrometry and high-throughput sequencing technologies to identify novel peptides. The aim of this study was to identify potential protein biomarkers for clinical treatment of HNSCC. Read More

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http://dx.doi.org/10.1177/0963689720929308DOI Listing

The Effect of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells' Therapy in the Treatment of Chronic Posttraumatic Spinal Cord Injury in a Domestic Ferret Patient.

Cell Transplant 2020 Jan-Dec;29:963689720928982

Pulawian Veterinary Center, Poland.

Spinal cord injury (SCI) is considered as one of the most problematic neurological conditions requiring specialized clinical intervention. Taking into account that SCI is characterized by extensive loss of nerve cells, stem cell-based therapy seems to be a reasonable modern strategy to the treatment of SCI. The presented case report describes for the first time experimental treatment with the use of autologous adipose tissue-derived mesenchymal stem cells (ADSCs) of the chronic posttraumatic SCI in a domestic ferret patient with paresis of back legs. Read More

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http://dx.doi.org/10.1177/0963689720928982DOI Listing

Validation of MicroRNA-188-5p Inhibition Power on Tumor Cell Proliferation in Papillary Thyroid Carcinoma.

Cell Transplant 2020 Jan-Dec;29:963689720918300

Medical Department, The Second Hospital of Wuhan Iron and Steel Group, Wuhan, China.

Given the crucial role of microRNAs in the cellular proliferation of various types of cancers, we aimed to analyze the expression and function of a cellular proliferation-associated miR-188-5p in papillary thyroid carcinoma (PTC). Here we demonstrate that miR-188-5p is downregulated in PTC tumor tissues compared with the associated noncancerous tissues. We also validate that the miR-188-5p overexpression suppressed the PTC cancer cell proliferation. Read More

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http://dx.doi.org/10.1177/0963689720918300DOI Listing

CircUBAP2 Inhibits Proliferation and Metastasis of Clear Cell Renal Cell Carcinoma via Targeting miR-148a-3p/FOXK2 Pathway.

Cell Transplant 2020 Jan-Dec;29:963689720925751

Department of Urology, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, China.

Clear cell renal cell carcinoma (ccRCC) is the prominent histological subtype of renal cell carcinoma (RCC) with high incidence of local recurrence and distant metastasis. It has been documented that circular ribonucleic acids (circRNAs) play crucial roles in the development of cancers; however, study on exploring the role of circRNAs in ccRCC still remains limited. In the present study, we aimed to evaluate the biological function of a novel circRNA UBAP2 (circUBAP2) in ccRCC and the underlying mechanism. Read More

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http://dx.doi.org/10.1177/0963689720925751DOI Listing
May 2020
3.570 Impact Factor

RNA Sequencing of Human Peripheral Nerve in Response to Injury: Distinctive Analysis of the Nerve Repair Pathways.

Cell Transplant 2020 Jan-Dec;29:963689720926157

Department of Neuroscience, University of Kentucky Medical Center, Lexington, KY, USA.

The development of regenerative therapies for central nervous system diseases can likely benefit from an understanding of the peripheral nervous system repair process, particularly in identifying potential gene pathways involved in human nerve repair. This study employed RNA sequencing (RNA-seq) technology to analyze the whole transcriptome profile of the human peripheral nerve in response to an injury. The distal sural nerve was exposed, completely transected, and a 1 to 2 cm section of nerve fascicles was collected for RNA-seq from six participants with Parkinson's disease, ranging in age between 53 and 70 yr. Read More

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http://dx.doi.org/10.1177/0963689720926157DOI Listing
May 2020
3.570 Impact Factor

Evaluation of Strategies Aimed at Improving Liver Progenitor Cell Rolling and Subsequent Adhesion to the Endothelium.

Cell Transplant 2020 Jan-Dec;29:963689720912707

Laboratory of Pediatric Hepatology and Cell Therapy, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Bruxelles, Belgium.

Adult-derived human liver stem/progenitor cells (ADHLSCs) are a promising alternative to orthotopic liver transplantation in the treatment of inborn errors of metabolism. However, as is the case with many mesenchymal stromal cells, ADHLSCs have shown a low level of engraftment, which could be explained by the fact that they lack expression of selectin ligand and LFA-1 and only slightly express VLA- 4, molecules that have been shown to be involved in cell adhesion to the endothelium. In this paper, we have investigated strategies to increase their rolling and adhesion during the homing process by (1) adding a selectin ligand (Sialyl Lewis X) to their surface using biotinyl--hydroxy-succinimide-streptavidin bridges, and (2) protecting the adhesion proteins from trypsinization-induced damage using a thermosensitive polymer for cell culture and a nonenzymatic cell dissociation solution (CDS) for harvest. Read More

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http://dx.doi.org/10.1177/0963689720912707DOI Listing

Semi-Automated Assessment of Human Islet Viability Predicts Transplantation Outcomes in a Diabetic Mouse Model.

Cell Transplant 2020 Jan-Dec;29:963689720919444

Department of Translational Research & Cellular Therapeutics, Beckman Research Institute of City of Hope, Duarte, CA, USA.

In clinical and experimental human pancreatic islet transplantations, establishing pretransplant assessments that accurately predict transplantation outcomes is crucial. Conventional viability assessment that relies on manual counting of viable islets is a routine pretransplant assessment. However, this method does not correlate with transplantation outcomes; to improve the method, we recently introduced a semi-automated method using imaging software to objectively determine area-based viability. Read More

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http://dx.doi.org/10.1177/0963689720919444DOI Listing

Cardiovascular Changes Associated with Hypertensive Heart Disease and Aging.

Cell Transplant 2020 Jan-Dec;29:963689720920830

Department of Biomedical Engineering, School of Medicine and School of Engineering, The University of Alabama at Birmingham, USA.

Cardiovascular diseases are the leading cause of mortality and morbidity worldwide and account for more than 17.9 million deaths (World Health Organization report). Hypertension and aging are two major risk factors for the development of cardiac structural and functional abnormalities. Read More

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http://dx.doi.org/10.1177/0963689720920830DOI Listing

The Morphological Analysis of Cells in the Bronchoscopic Brushing and TBNA of Patients with Lung Adenocarcinoma.

Cell Transplant 2020 Jan-Dec;29:963689720923599

Department of Pathology, The First Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China.

Biopsy, brushing, and transbronchial needle aspiration (TBNA) are the most common methods for diagnosis of lung adenocarcinoma and are taken during the same diagnostic bronchoscopic procedure. However, it is not clear what the morphological diagnostic criteria of cytology by brushing or TBNA are. A retrospective analysis was performed on 136 patients who underwent video bronchoscopy examination for diagnostic purposes. Read More

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http://dx.doi.org/10.1177/0963689720923599DOI Listing

CORM-2 Pretreatment Attenuates Inflammation-mediated Islet Dysfunction.

Cell Transplant 2020 Jan-Dec;29:963689720903691

Organ Transplant Center, Tianjin First Central Hospital, Nankai University, Tianjin, China.

During the process of human islet isolation a cascade of stressful events are triggered and negatively influence islet yield, viability, and function, including the production of proinflammatory cytokines and activation of apoptosis. Carbon monoxide-releasing molecule 2 (CORM-2) is a donor of carbon monoxide (CO) and can release CO spontaneously. Accumulating studies suggest that CORM-2 exerts cytoprotective and anti-inflammatory properties. Read More

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http://dx.doi.org/10.1177/0963689720903691DOI Listing

Extracellular Vesicles Derived from Mesenchymal Stem Cells Recover Fertility of Premature Ovarian Insufficiency Mice and the Effects on their Offspring.

Cell Transplant 2020 Jan-Dec;29:963689720923575

Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, Hefei, China.

It has been reported that extracellular vesicles (EVs) derived from human umbilical cord mesenchymal stem cells (HUCMSCs) can promote the proliferative and secretive functions of granulosa cells. In vivo study further demonstrated that EVs derived from HUCMSCs can not only promote the angiogenesis of ovarian tissue but also restore the function of an ovary of chemically induced premature ovarian insufficiency (POI) mice. However, no study investigates the effects of HUCMSCs derived EVs on fertility recovery of POI mice and evaluating their offspring. Read More

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http://dx.doi.org/10.1177/0963689720923575DOI Listing
May 2020
3.570 Impact Factor

Evaluation and Transplantation of Human Corneal Endothelial Cells Cultured on Biocompatible Carriers.

Cell Transplant 2020 Jan-Dec;29:963689720923577

Netherlands Institute for Innovative Ocular Surgery, Rotterdam, the Netherlands.

Corneal transplantation is currently the only effective treatment option for dysfunctional corneal endothelial cells (CEC). In this study, we test the surgical potential of cultivated human corneal endothelial cells (hCEC) on human anterior lens capsule (HALC), LinkCell™ bioengineered collagen sheets of 20-µm thickness (LK20), and denuded Descemet membrane (dDM) as tissue-engineered grafts for Descemet membrane (DM) endothelial keratoplasty (DMEK) to bypass the problem of donor tissue availability. Primary hCEC cultured on all carriers formed a monolayer of tightly packed cells with a high cell viability rate (96% ± 4%). Read More

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http://dx.doi.org/10.1177/0963689720923577DOI Listing

Long Noncoding RNA PCED1B-AS1 Promotes the Warburg Effect and Tumorigenesis by Upregulating HIF-1α in Glioblastoma.

Cell Transplant 2020 Jan-Dec;29:963689720906777

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Henan, China.

Accumulating evidence suggests that long noncoding RNA (lncRNA) functions as a critical regulator in cancer biology. Here, we characterized the role of lncRNA PCED1B antisense RNA 1 (PCED1B-AS1) in glioblastoma (GBM). PCED1B-AS1 was notably upregulated in GBM tissues and cell lines and closely associated with larger tumor size and higher grade. Read More

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http://dx.doi.org/10.1177/0963689720906777DOI Listing

Mechanical Stimulation of Fibroblasts by Extracorporeal Shock Waves: Modulation of Cell Activation and Proliferation Through a Transient Proinflammatory Milieu.

Cell Transplant 2020 Jan-Dec;29:963689720916175

Department of Biomedical Sciences, University of Sassari, Sassari, Italy.

Extracorporeal shock waves (ESWTs) are "mechanical" waves, widely used in regenerative medicine, including soft tissue wound repair. Although already being used in the clinical practice, the mechanism of action underlying their biological activities is still not fully understood. In the present paper we tried to elucidate whether a proinflammatory effect may contribute to the regenerative potential of shock waves treatment. Read More

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http://dx.doi.org/10.1177/0963689720916175DOI Listing

In Vitro Evaluation of a Novel Synthetic Bilirubin Analog as an Antioxidant and Cytoprotective Agent for Pancreatic Islet Transplantation.

Cell Transplant 2020 Jan-Dec;29:963689720906417

Department of Small Animal Clinical Sciences, University of Florida, Gainesville, FL, USA.

Bilirubin is a natural cytoprotective agent and physiologic doses have proven to be beneficial in various models of organ and cellular transplantation. Recently, we showed that bilirubin has protective effects in models of pancreatic islet transplantation, preventing cell death associated with islet stress and suppressing the release of damage-associated molecular patterns. Despite these promising therapeutic attributes, the natural bilirubin used in these research studies is animal-derived (porcine), making it unsuitable for clinical application. Read More

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http://dx.doi.org/10.1177/0963689720906417DOI Listing

Comparative Efficacy and Clinical Outcomes of Haploidentical Stem Cell Transplantation to Other Stem Sources for Treatment in Acute Myeloid Leukemia and Myelodysplastic Syndrome Patients: A Systematic Review and Meta-Analysis.

Cell Transplant 2020 Jan-Dec;29:963689720904965

Division of Hematology, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.

The use of allogeneic hematopoietic stem cell transplantation (HSCT) is recommended during the first complete remission of acute myeloid leukemia (AML) and high-risk myelodysplastic syndrome (MDS). However, only 30% of these cases have fully matched sibling donors (MSDs). Alternatively, matched unrelated donors (MUDs) and haploidentical (haplo) donors from first-degree relatives increase the access to transplantation, with some reported differences in outcomes. Read More

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http://dx.doi.org/10.1177/0963689720904965DOI Listing
April 2020
3.570 Impact Factor

Molecular Aspects of Adipose-Derived Stromal Cell Senescence in a Long-Term Culture: A Potential Role of Inflammatory Pathways.

Cell Transplant 2020 Jan-Dec;29:963689720917341

Department of Regenerative Medicine, Cell and Tissue Bank, Chair of Urology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.

Long-term culture of mesenchymal stromal/stem cells leads to their senescence. It is very important to define the maximal passage to which the mesenchymal stromal/stem cells maintain their regenerative properties and can be used for cellular therapies and construction of neo-organs for clinical application. Adipose-derived stromal/stem cells were isolated from porcine adipose tissue. Read More

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http://dx.doi.org/10.1177/0963689720917341DOI Listing

Factors Associated with Costs in Chimeric Antigen Receptor T-Cell Therapy for Patients with Relapsed/Refractory B-Cell Malignancies.

Cell Transplant 2020 Jan-Dec;29:963689720919434

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Chimeric antigen receptor T cells (CAR-Ts) constitute a novel therapeutic strategy for relapsed/refractory B-cell malignancies. CAR-T therapy has been extensively applied in the clinical setting; however, few systematic studies have evaluated the cost of CAR-T treatment. This study was conducted to evaluate the total cost and cost structure of CAR-T therapy and identify potential risk factors leading to increased costs. Read More

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http://dx.doi.org/10.1177/0963689720919434DOI Listing

Transplanted Human Neural Progenitor Cells Attenuate Motor Dysfunction and Lengthen Longevity in a Rat Model of Ataxia.

Cell Transplant 2020 Jan-Dec;29:963689720920275

Department of Biology, California State University, Northridge, CA, USA.

The Han Wistar (sHW) rat serves as a model for human ataxia presenting symptoms of motor deterioration, weight loss, shortened lifespan, and Purkinje neuron loss. Past studies revealed that human neural progenitor cells (NPCs) improved ataxic symptoms at 20 d posttransplantation in sHW rats. In this study, we investigated the fate and longer-term effectiveness of these transplanted NPCs. Read More

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http://dx.doi.org/10.1177/0963689720920275DOI Listing

The Role of LncRNA H19 in MAPK Signaling Pathway Implicated in the Progression of Bronchopulmonary Dysplasia.

Cell Transplant 2020 Jan-Dec;29:963689720918294

Foshan Chancheng Central Hospital, Foshan, Guangdong, China.

Bronchopulmonary dysplasia (BPD), also known as neonatal chronic lung disease, is an important cause of respiratory illness in preterm newborns that results in significant morbidity and mortality. Long noncoding RNAs (lncRNAs) have been discovered with many biological functions. However, the role of lncRNAs in the pathogenesis of BPD remains poorly understood. Read More

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http://dx.doi.org/10.1177/0963689720918294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289048PMC

GDNF Promotes Survival and Therapeutic Efficacy of Human Adipose-Derived Mesenchymal Stem Cells in a Mouse Model of Parkinson's Disease.

Cell Transplant 2020 Jan-Dec;29:963689720908512

Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Mesenchymal stem cell (MSC)-based regenerative therapy is regarded as a promising strategy for the treatment of Parkinson's disease (PD). However, MSC components may exhibit poor intracranial survivability, particularly in the later stages following cell transplantation, limiting their potential curative effect and also clinical applications. Glial cell line-derived neurotrophic factor (GDNF), which encompasses a variety of transforming growth factor beta super family members, has been reported to enhance motor function and exert neuroprotective effects. Read More

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http://dx.doi.org/10.1177/0963689720908512DOI Listing

Islet Transplantation to the Anterior Chamber of the Eye-A Future Treatment Option for Insulin-Deficient Type-2 Diabetics? A Case Report from a Nonhuman Type-2 Diabetic Primate.

Cell Transplant 2020 Jan-Dec;29:963689720913256

Translational Pre-Clinical Model Platform, Singapore Eye Research Institute (SERI), Singapore.

Replacement of the insulin-secreting beta cells through transplantation of pancreatic islets to the liver is a promising treatment for type-1 diabetes. However, low oxygen tension, shear stress, and the induction of inflammation lead to significant islet dysfunction and loss. The anterior chamber of the eye (ACE) has gained considerable interest and represents an alternative therapeutic islet transplantation site because of its accessibility, high oxygen tension, and immune-privileged milieu. Read More

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http://dx.doi.org/10.1177/0963689720913256DOI Listing

Transplantation of sh-miR-199a-5p-Modified Olfactory Ensheathing Cells Promotes the Functional Recovery in Rats with Contusive Spinal Cord Injury.

Cell Transplant 2020 Jan-Dec;29:963689720916173

Department of Orthopaedics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

MicroRNAs (miRNAs) function as gene expression switches, and participate in diverse pathophysiological processes of spinal cord injury (SCI). Olfactory ensheathing cells (OECs) can alleviate pathological injury and facilitate functional recovery after SCI. However, the mechanisms by which OECs restore function are not well understood. Read More

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http://dx.doi.org/10.1177/0963689720916173DOI Listing

A Likely Role for a Novel Cell Therapeutic Target of Transforming Growth Factor-β1 on Radiation Pneumonitis in Lung and Nasopharyngeal Cancer Patients.

Cell Transplant 2020 Jan-Dec;29:963689720914245

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

The association between the polymorphism of transforming growth factor (TGF)-β1 and risk of radiation pneumonitis has been extensively investigated; however, conclusive results were unavailable. Eligible studies were identified from the database of Medline, Web of Science, EMBASE, and CNKI (China Knowledge Resource Integrated Database) up to September 2019. The odds ratio (OR) and 95% confidence interval (95% CI) were used to assess the strength of the relationship. Read More

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http://dx.doi.org/10.1177/0963689720914245DOI Listing

Establishment of Immortalized Laryngeal Epithelial Cells Transfected with Bmi1.

Cell Transplant 2020 Jan-Dec;29:963689720908198

Department of Otolaryngology, Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Primary laryngeal epithelial cells are essential to exploring the mechanisms of laryngeal and voice disorders; however, they are difficult to study and apply because of their limited life span. The purpose of this study was to develop a stable and reliable model for the comprehensive study of the pathogenesis of laryngeal and voice diseases. The pLVTHM-Bmi1 plasmid was constructed and used to immortalize primary laryngeal epithelial cells by lentiviral infection. Read More

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http://dx.doi.org/10.1177/0963689720908198DOI Listing

Low-Energy Shockwave Treatment Promotes Endothelial Progenitor Cell Homing to the Stenotic Pig Kidney.

Cell Transplant 2020 Jan-Dec;29:963689720917342

Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.

Endothelial progenitor cells (EPCs) patrols the circulation and contributes to endothelial cell regeneration. Atherosclerotic renal artery stenosis (ARAS) induces microvascular loss in the stenotic kidney (STK). Low-energy shockwave therapy (SW) can induce angiogenesis and restore the STK microcirculation, but the underlying mechanism remains unclear. Read More

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http://dx.doi.org/10.1177/0963689720917342DOI Listing

Transplantation of Endothelial Progenitor Cells in the Treatment of Coronary Artery Microembolism in Rats.

Cell Transplant 2020 Jan-Dec;29:963689720912688

Department of Cardiology, Beijing Tsinghua Changgung Hospital, China.

As the impairment of myocardial microenvironments due to coronary microembolization (CME) compromises the treatment effect of percutaneous coronary intervention and leads to adverse prognosis, we hypothesized that endothelial progenitor cells (EPCs) transplantation could improve cardiac function in the condition of CME. Low- (2 × 10) and high- (2 × 10) dose rat bone marrow-derived EPCs were transplanted in a model of CME. To develop a CME model, rats were injected with autologous micro-blood-clots into the left ventricle. Read More

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http://dx.doi.org/10.1177/0963689720912688DOI Listing

for Stem Cell Therapy and Medical Use: Pros or Cons?

Cell Transplant 2020 Jan-Dec;29:963689720907565

Buddhist Tzu Chi Bioinnovation Center, Tzu Chi Foundation, Hualien, Republic of China.

Stem cell transplantation is a fast-developing technique, which includes stem cell isolation, purification, and storage, and it is in high demand in the industry. In addition, advanced applications of stem cell transplantation, including differentiation, gene delivery, and reprogramming, are presently being studied in clinical trials. In contrast to somatic cells, stem cells are self-renewing and have the ability to differentiate; however, the molecular mechanisms remain unclear. Read More

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http://dx.doi.org/10.1177/0963689720907565DOI Listing

The Neuronal Regeneration of Adult Zebrafish After Spinal Cord Injury Is Enhanced by Transplanting Optimized Number of Neural Progenitor Cells.

Cell Transplant 2020 Jan-Dec;29:963689720903679

Institute of Biomedical Science, Mackay Medical College, New Taipei City.

Cell transplantation is commonly used to study the regeneration and repair of the nervous system in animals. However, a technical platform used to evaluate the optimum number of transplanted cells in the recipient's spinal cord is little reported. Therefore, to develop such platform, we used a zebrafish model, which has transparent embryos, and transgenic line , which generates green fluorescent protein (GFP)-expressing cells in the central nervous system under hypoxic stress. Read More

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http://dx.doi.org/10.1177/0963689720903679DOI Listing

Bone Marrow-Derived Mesenchymal Stem Cells Restored High-Fat-Fed Induced Hyperinsulinemia in Rats at Early Stage of Type 2 Diabetes Mellitus.

Cell Transplant 2020 Jan-Dec;29:963689720904628

Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei Chronic Wound and Diabetic Foot Clinical Research Center, Wuhan, China.

Numerous studies have proposed the transplantation of mesenchymal stem cells (MSCs) in the treatment of typical type 2 diabetes mellitus (T2DM). We aimed to find a new strategy with MSC therapy at an early stage of T2DM to efficiently prevent the progressive deterioration of organic dysfunction. Using the high-fat-fed hyperinsulinemia rat model, we found that before the onset of typical T2DM, bone marrow-derived MSCs (BM-MSCs) significantly attenuated rising insulin with decline in glucose as well as restored lipometabolic disorder and liver dysfunction. Read More

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http://dx.doi.org/10.1177/0963689720904628DOI Listing

The Effect of Recovery Warm-up Time Following Cold Storage on the Dynamic Glucose-stimulated Insulin Secretion of Isolated Human Islets.

Cell Transplant 2020 Jan-Dec;29:963689720908278

Diabetes Research Institute, Miller School of Medicine, University of Miami, FL, USA.

Standardized islet characterization assays that can provide results in a timely manner are essential for successful islet cell transplantation. A critical component of islet cell quality is β-cell function, and perifusion-based assessments of dynamic glucose-stimulated insulin secretion (GSIS) are the most informative method to assess this, as they provide the most complex in vitro evaluation of GSIS. However, protocols used vary considerably among centers and investigators as they often use different low- and high-glucose concentrations, exposure-times, flow-rates, oxygen concentrations, islet numbers, analytical methods, measurement units, and instruments, which result in different readouts and make comparisons across platforms difficult. Read More

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http://dx.doi.org/10.1177/0963689720908278DOI Listing