205,662 results match your criteria Cell Stress & Chaperones [Journal]


Heterologous expression of an alternative oxidase from Moniliophthora perniciosa in Saccharomyces cerevisiae: antioxidant function and in vivo platform for the study of new drugs against witches' broom disease.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Laboratório de Biologia Molecular e Biotecnologia Industrial de Microrganismos, Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. Electronic address:

The fungus Moniliophthora perniciosa is the causal agent of witches' broom disease (WBD), one of the most devastating diseases of cacao, the chocolate tree. Many strategies to control WBD have been tested so far, including the use of agrochemicals such as the strobilurins. Strobilurins are fungicides of the QoI family, and they are used in the control of a wide array of fungal diseases in many different crops, including cereals, field crops, fruits, tree nuts, and vegetables. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fgb.2019.02.006DOI Listing
February 2019

Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut.

Fish Shellfish Immunol 2019 Feb 19. Epub 2019 Feb 19.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; CAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266400, China. Electronic address:

In order to understand the mediation function of surface proteins in probiotic effects executed by Lactobacillus pentosus HC-2 in midgut of Litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal HC-2 or with stripped surface proteins HC-2 by lithium chloride (LiCl) treatment. The results showed that the shrimp fed with normal HC-2 produced much higher immune and digestion related enzymes than the control group or LiCl-treated HC-2 group to defense the Vibrio parahaemolyticus E1 infection. We obtained total over 275,099 unigenes from L. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2019.02.027DOI Listing
February 2019

Reactive cholangiocytes differentiate into proliferative hepatocytes with efficient DNA repair in mice with chronic liver injury.

J Hepatol 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Hepato-gastroenterology, Institut de Recherche Expérimentale et Clinique, Université catholique de Louvain, Brussels, Belgium. Electronic address:

Background And Aim: Chronic liver diseases are characterized by expansion of the small immature cholangiocytes - a mechanism named ductular reaction (DR) - which have the capacity to differentiate in hepatocytes. We investigated the kinetics of DR differentiation into hepatocytes as well as several important features as functional maturity, clonal expansion and resistant to stress of the newly formed hepatocytes in mice with long-term liver damage.

Methods: We tracked cholangiocytes using osteopontin-iCreER and hepatocytes with AAV8-TBG-Cre. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhep.2019.02.003DOI Listing
February 2019

Mechanical influences on Cardiovascular Differentiation and Disease Modeling.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037. Electronic address:

Tissues are continuously exposed to forces in vivo, whether from fluid pressure in an artery from our blood or compressive forces on joints from our body weight. The forces that cells are exposed to arise almost immediately after conception; it is therefore important to understand how forces shape stem cell differentiation into lineage committed cells, how they help organize cells into tissues, and how forces can cause or exacerbate disease. No tissue is exempt, but cardiovascular tissues in particular are exposed to these forces. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yexcr.2019.02.019DOI Listing
February 2019

Omentin protects H9c2 cells against docetaxel cardiotoxicity.

PLoS One 2019 22;14(2):e0212782. Epub 2019 Feb 22.

Cardiology Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela and Health Research Institute, University Clinical Hospital of Santiago de Compostela-Santiago de Compostela, Spain.

Background: Association between obesity and cardiovascular diseases is well known, however increased susceptibility of obese patients to develop several cancer types is not so commonly known. Current data suggest that poorer overall survival in cancer patients might be associated to non-cancer-related causes such as higher risk of cardiotoxicity in obese patients treated with chemotherapeutic agents. Omentin, a novel adipokine decreased in obesity, is actually in the spotlight due to its favourable effects on inflammation, glucose homeostasis and cardiovascular diseases. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212782PLOS
February 2019

Alteration of mRNA abundance, oxidation products and antioxidant enzyme activities during oocyte ageing in common carp Cyprinus carpio.

PLoS One 2019 22;14(2):e0212694. Epub 2019 Feb 22.

University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic.

Oocyte ageing is the most important factor affecting egg quality of several fish species after ovulation. Oxidative stress has been proposed as the initiator of the oocyte ageing process in other vertebrates. To identify the role of oxidative stress and apoptosis on the progress of oocyte ageing in the common carp Cyprinus carpio, changes in the relative mRNA abundance of selected transcripts were examined. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212694PLOS
February 2019

Metacaspase gene family in Rosaceae genomes: Comparative genomic analysis and their expression during pear pollen tube and fruit development.

PLoS One 2019 22;14(2):e0211635. Epub 2019 Feb 22.

School of Life Sciences, Anhui Agricultural University, Hefei, China.

Metacaspase (MC), which is discovered gene family with distant caspase homologs in plants, fungi, and protozoa, may be involved in programmed cell death (PCD) processes during plant development and respond abiotic and biotic stresses. To reveal the evolutionary relationship of MC gene family in Rosaceae genomes, we identified 8, 7, 8, 12, 12, and 23 MC genes in the genomes of Fragaria vesca, Prunus mume, Prunus persica, Pyrus communis, Pyrus bretschneideri and Malus domestica, respectively. Phylogenetic analysis suggested that the MC genes could be grouped into three clades: Type I*, Type I and Type II, which was supported by gene structure and conserved motif analysis. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211635PLOS
February 2019

Role of deformable cancer cells on wall shear stress-associated-VEGF secretion by endothelium in microvasculature.

PLoS One 2019 22;14(2):e0211418. Epub 2019 Feb 22.

Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America.

Endothelial surface layer (glycocalyx) is the major physiological regulator of tumor cell adhesion to endothelium. Cancer cells express vascular endothelial growth factor (VEGF) which increases the permeability of a microvessel wall by degrading glycocalyx. Endothelial cells lining large arteries have also been reported, in vitro and in vivo, to mediate VEGF expression significantly under exposure to high wall shear stress (WSS) > 0. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211418PLOS
February 2019

Induced aneuploidy in neural stem cells triggers a delayed stress response and impairs adult life span in flies.

PLoS Biol 2019 Feb 22;17(2):e3000016. Epub 2019 Feb 22.

Instituto Gulbenkian de Ciência, Oeiras, Portugal.

Studying aneuploidy during organism development has strong limitations because chronic mitotic perturbations used to generate aneuploidy usually result in lethality. We developed a genetic tool to induce aneuploidy in an acute and time-controlled manner during Drosophila development. This is achieved by reversible depletion of cohesin, a key molecule controlling mitotic fidelity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.pbio.3000016DOI Listing
February 2019

C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/β-catenin and ERK signaling in streptozocin-induced diabetic nephropathy.

FASEB J 2019 Feb 22:fj201801865RR. Epub 2019 Feb 22.

Ministry of Education (MOE) Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

Previous studies have reported the pathogenic role of C-reactive protein (CRP) during diabetic kidney disease (DKD) in human CRP transgenic and Crp mice. However, because humans and mice have inverse acute phase expression patterns of CRP and serum amyloid P component, this could lead to the inaccurate evaluation of CRP function with the above-mentioned CRP transgenic mouse. But different from mice, rats have the same acute phase protein expression pattern as human, which might avoid this problem and be a better choice for CRP function studies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801865RRDOI Listing
February 2019

Knockout of receptor for advanced glycation end-products attenuates age-related renal lesions.

Aging Cell 2019 Feb 22:e12850. Epub 2019 Feb 22.

U995 - Lille Inflammation Research International Center, INSERM, CHU Lille, University of Lille, Lille, France.

Pro-aging effects of endogenous advanced glycation end-products (AGEs) have been reported, and there is increasing interest in the pro-inflammatory and -fibrotic effects of their binding to RAGE (the main AGE receptor). The role of dietary AGEs in aging remains ill-defined, but the predominantly renal accumulation of dietary carboxymethyllysine (CML) suggests the kidneys may be particularly affected. We studied the impact of RAGE invalidation and a CML-enriched diet on renal aging. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/acel.12850DOI Listing
February 2019

The effects of dynamic compressive loading on human mesenchymal stem cell osteogenesis in the stiff layer of a bilayer hydrogel.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Chemical and Biological Engineering, University of Colorado, Boulder, CO.

Bilayer hydrogels with a soft cartilage-like layer and a stiff bone-like layer embedded with human mesenchymal stem cells (hMSCs) are promising for osteochondral tissue engineering. The goals of this work were to evaluate the effects of dynamic compressive loading (2.5% applied strain, 1 Hz) on osteogenesis in the stiff layer and spatially map local mechanical responses (strain, stress, hydrostatic pressure and fluid velocity). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/term.2827DOI Listing
February 2019

Stress-induced mRNP granules: Form and function of processing bodies and stress granules.

Wiley Interdiscip Rev RNA 2019 Feb 21:e1524. Epub 2019 Feb 21.

Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California.

In response to stress, cells must quickly reprogram gene expression to adapt and survive. This is achieved in part by altering levels of mRNAs and their translation into proteins. Recently, the formation of two stress-induced messenger ribonucleoprotein (mRNP) assemblies named stress granules and processing bodies has been postulated to directly impact gene expression during stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/wrna.1524DOI Listing
February 2019

Inhibition of P53/miR-34a improves diabetic endothelial dysfunction via activation of SIRT1.

J Cell Mol Med 2019 Feb 22. Epub 2019 Feb 22.

Department of Toxicology and Nutrition, School of Public Health, Shandong University, Jinan, Shandong, China.

Endothelial dysfunction contributes to diabetic macrovascular complications, resulting in high mortality. Recent findings demonstrate a pathogenic role of P53 in endothelial dysfunction, encouraging the investigation of the effect of P53 inhibition on diabetic endothelial dysfunction. Thus, high glucose (HG)-treated endothelial cells (ECs) were subjected to pifithrin-α (PFT-α)-a specific inhibitor of P53, or P53-small interfering RNA (siRNA), both of which attenuated the HG-induced endothelial inflammation and oxidative stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jcmm.14253DOI Listing
February 2019

Methyl B12 protects PC12 cells against cytotoxicity induced by Aβ.

J Cell Biochem 2019 Feb 21. Epub 2019 Feb 21.

Institute of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, China.

Alzheimer's disease (AD) is the most common aging-associated dementia. The population of AD patients is increasing as the world age grows. Currently, there is no cure for AD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcb.28475DOI Listing
February 2019

VEGF-A promotes angiogenesis after acute myocardial infarction through increasing ROS production and enhancing ER stress-mediated autophagy.

J Cell Physiol 2019 Feb 21. Epub 2019 Feb 21.

Department of Pathophysiology, School of Basic Medical Science, Central South University, Changsha, Hunan, China.

Proangiogenesis is generally regarded as an effective approach for treating ischemic heart disease. Vascular endothelial growth factor (VEGF)-A is a strong and essential proangiogenic factor. Reactive oxygen species (ROS), endoplasmic reticulum (ER) stress, and autophagy are implicated in the process of angiogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcp.28395DOI Listing
February 2019

The potential contribution of impaired brain glucose metabolism to congenital Zika syndrome.

J Anat 2019 Feb 21. Epub 2019 Feb 21.

Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.

The Zika virus (ZIKV) became a major worldwide public concern in 2015 due to the congenital syndrome which presents the highest risk during the first trimester of pregnancy and includes microcephaly and eye malformations. Several cellular, genetic and molecular studies have shown alterations in metabolic pathways, endoplasmic reticulum (ER) stress, immunity and dysregulation of RNA and energy metabolism both in vivo and in vitro. Here we summarise the main metabolic complications, with a particular focus on the possibility that brain energy metabolism is altered following ZIKV infection, contributing to developmental abnormalities. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/joa.12959DOI Listing
February 2019

Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction.

Eur Cell Mater 2019 Feb 22;37:113-133. Epub 2019 Feb 22.

Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Krankenhausstraße 7, A-4010 Linz,

The prerequisite for a successful clinical use of autologous adipose-tissue-derived cells is the highest possible regenerative potential of the applied cell population, the stromal vascular fraction (SVF). Current isolation methods depend on high enzyme concentration, lysis buffer, long incubation steps and mechanical stress, resulting in single cell dissociation. The aim of the study was to limit cell manipulation and obtain a derivative comprising therapeutic cells (microtissue-SVF) without dissociation from their natural extracellular matrix, by employing a gentle good manufacturing practice (GMP)-grade isolation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.22203/eCM.v037a08DOI Listing
February 2019

Does co-transcriptional regulation of alternative splicing mediate plant stress responses?

Nucleic Acids Res 2019 Feb 22. Epub 2019 Feb 22.

School of Human and Life Sciences, Canterbury Christ Church University, Canterbury, CT1 1QU, UK.

Plants display exquisite control over gene expression to elicit appropriate responses under normal and stress conditions. Alternative splicing (AS) of pre-mRNAs, a process that generates two or more transcripts from multi-exon genes, adds another layer of regulation to fine-tune condition-specific gene expression in animals and plants. However, exactly how plants control splice isoform ratios and the timing of this regulation in response to environmental signals remains elusive. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkz121DOI Listing
February 2019

Genome-wide characterization, molecular evolution and expression profiling of the metacaspases in potato ( L.).

Heliyon 2019 Feb 7;5(2):e01162. Epub 2019 Feb 7.

Plant Cell and Molecular Biology Laboratory, Department of Botany, Faculty of Science, The M.S. University of Baroda, Vadodara 390 002, Gujarat, India.

Metacaspases are distant relatives of animal caspases found in plants, protozoa and fungi. Some recent studies have demonstrated that metacaspases are involved in regulating the developmental and environmentally induced programmed cell death in plants. In this study, we identified metacaspase gene family in potato ( L. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.heliyon.2019.e01162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370574PMC
February 2019

Novel Pathogenic Variant p.Glu190Lys in a Case of Chronic Pancreatitis.

Front Genet 2019 6;10:46. Epub 2019 Feb 6.

Center for Exocrine Disorders, Department of Molecular and Cell Biology, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, United States.

Mutations in the (serine protease 1) gene encoding human cationic trypsinogen cause hereditary pancreatitis or may be associated with sporadic chronic pancreatitis. The mutations exert their pathogenic effect either by increasing intra-pancreatic trypsinogen activation (trypsin pathway) or by causing proenzyme misfolding and endoplasmic reticulum stress (misfolding pathway). Here we report a novel heterozygous c. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2019.00046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375306PMC
February 2019

Signaling Overview of Plant Somatic Embryogenesis.

Front Plant Sci 2019 7;10:77. Epub 2019 Feb 7.

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Mexico.

Somatic embryogenesis (SE) is a means by which plants can regenerate bipolar structures from a somatic cell. During the process of cell differentiation, the explant responds to endogenous stimuli, which trigger the induction of a signaling response and, consequently, modify the gene program of the cell. SE is probably the most studied plant regeneration model, but to date it is the least understood due to the unclear mechanisms that occur at a cellular level. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2019.00077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375091PMC
February 2019

CrfC Protein, a Nucleoid Partition Factor, Localizes to Nucleoid Poles via the Activities of Specific Nucleoid-Associated Proteins.

Front Microbiol 2019 7;10:72. Epub 2019 Feb 7.

Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.

The CrfC protein is an important regulator of nucleoid positioning and equipartition. Previously we revealed that CrfC homo-oligomers bind the clamp, a DNA-binding subunit of the DNA polymerase III holoenzyme, promoting colocalization of the sister replication forks, which ensures the nucleoid equipartition. In addition, CrfC localizes at the cell pole-proximal loci via an unknown mechanism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2019.00072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374313PMC
February 2019

Herpes Virus Reactivation in Astronauts During Spaceflight and Its Application on Earth.

Front Microbiol 2019 7;10:16. Epub 2019 Feb 7.

Jes Tech, KBR Wyle Laboratories Houston, Houston, TX, United States.

Latent herpes virus reactivation has been demonstrated in astronauts during shuttle (10-16 days) and International Space Station (≥180 days) flights. Following reactivation, viruses are shed in the body fluids of astronauts. Typically, shedding of viral DNA is asymptomatic in astronauts regardless of mission duration; however, in some cases, live/infectious virus was recovered by tissue culture that was associated with atopic-dermatitis or skin lesions during and after spaceflight. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2019.00016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374706PMC
February 2019

Anti-thrombosis Effects and Mechanisms by Xueshuantong Capsule Under Different Flow Conditions.

Front Pharmacol 2019 7;10:35. Epub 2019 Feb 7.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

Xueshuantong capsule (XST) is a patented traditional Chinese medicine used for the prevention and treatment of thrombosis. The molecular mechanism of anti-thrombotic effect of XST was investigated through the cross-talk among the platelets/leukocytes, endothelial cells (ECs), and flow shear stress. The Bioflux 1000 system was used to generate two levels of shear stress conditions: 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2019.00035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374556PMC
February 2019

Molecular origin of AuNPs-induced cytotoxicity and mechanistic study.

Sci Rep 2019 Feb 21;9(1):2494. Epub 2019 Feb 21.

Department of Biomedical Engineering (BK21 plus), Dongguk University, Pildong 3-ga, Seoul, 04620, Korea.

Gold nanoparticles (AuNPs) with diverse physicochemical properties are reported to affect biological systems differently, but the relationship between the physicochemical properties of AuNPs and their biological effects is not clearly understood. Here, we aimed to elucidate the molecular origins of AuNP-induced cytotoxicity and their mechanisms, focusing on the surface charge and structural properties of modified AuNPs. We prepared a library of well-tailored AuNPs exhibiting various functional groups and surface charges. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-39579-3DOI Listing
February 2019

Premobilization of CD133+ cells by granulocyte colony- stimulating factor attenuates ischemic acute kidney injury induced by cardiopulmonary bypass.

Sci Rep 2019 Feb 21;9(1):2470. Epub 2019 Feb 21.

Department of Anesthesiology, West China Hospital, Sichuan University, 610041, Chengdu, China.

Ischemic acute kidney injury (IAKI) is a common but severe complication after a cardiopulmonary bypass (CPB). Multiple studies have demonstrated that peripheral CD133+ or differentiated cells are able to home and repair the damaged tissues, but the number of available CD133+ cells is limited, and no efficient method published previously to mobilize them immediately. We analyzed the relationship between CD133+ cells and renal function in CPB patients, in addition, the efficacy of granulocyte colony-stimulating factor (G-CSF) pre-mobilized CD133+ cells in treating of mouse IAKI model have been investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-38953-5DOI Listing
February 2019

Functional characterization of a putative DNA methyltransferase, EadM, in Xanthomonas axonopodis pv. glycines by proteomic and phenotypic analyses.

Sci Rep 2019 Feb 21;9(1):2446. Epub 2019 Feb 21.

Department of Integrative Plant Science, Chung-Ang University, Anseong, 17546, Republic of Korea.

Xanthomonas axonopodis pv. glycines (Xag) is a phytopathogenic bacterium causing bacterial pustule disease in soybean. Functions of DNA methyltransferases have been characterized in animal pathogenic bacteria, but are poorly understood in plant pathogens. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-38650-3DOI Listing
February 2019

BCL-2 family isoforms in apoptosis and cancer.

Cell Death Dis 2019 Feb 21;10(3):177. Epub 2019 Feb 21.

Hunter Medical Research Institute, Newcastle, NSW, Australia.

The BCl-2 family has long been identified for its role in apoptosis. Following the initial discovery of BCL-2 in the context of B-cell lymphoma in the 1980s, a number of homologous proteins have since been identified. The members of the Bcl-2 family are designated as such due to their BCL-2 homology (BH) domains and involvement in apoptosis regulation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-019-1407-6DOI Listing
February 2019

Nutraceutical effects of in age-related macular degeneration.

Aging (Albany NY) 2019 Feb 21. Epub 2019 Feb 21.

Department of Ophthalmology, Gavin Herbert Eye Institute, University of California Irvine, Irvine, CA 92697, USA.

Gaetrn i.e., Indian gooseberry/ Amla) (EO) has been used extensively as a nutraceutical in several diseases since it is known to boost immunity and offers numerous health benefits such as antioxidant, anti-inflammatory, and anti-aging effects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.101820DOI Listing
February 2019

Platelet-type 12-lipoxygenase deletion provokes a compensatory 12/15-lipoxygenase increase that exacerbates oxidative stress in islet β-cells.

J Biol Chem 2019 Feb 21. Epub 2019 Feb 21.

Pediatrics, Indiana University School of Medicine, United States.

In type 1 diabetes, an autoimmune event increases oxidative stress in islet β cells, giving rise to cellular dysfunction and apoptosis. Lipoxygenases are enzymes that catalyze the oxygenation of polyunsaturated fatty acids that can form lipid metabolites involved in several biological functions including oxidative stress. 12-lipoxygenase and 12/15-lipoxygenase are related but distinct enzymes that are expressed in pancreatic islets, but their relative contributions to oxidative stress in these regions are still being elucidated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.RA118.007102DOI Listing
February 2019

Metabolic, anti-apoptotic and immune evasion strategies of primary human myeloma cells indicate adaptations to hypoxia.

Mol Cell Proteomics 2019 Feb 21. Epub 2019 Feb 21.

Department of Analytical Chemistry, University of Vienna, Austria

Multiple Myeloma (MM) is an incurable plasma cell malignancy primarily localized within the bone marrow (BM). It develops from a premalignant stage, monoclonal gammopathy of undetermined significance (MGUS), often via an intermediate stage, smoldering MM (SMM). The mechanisms of MM progression have not yet been fully understood, all the more because patients with MGUS and SMM already carry similar initial mutations as found in MM cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1074/mcp.RA119.001390DOI Listing
February 2019

Regulatory T cells suppress excessive lipid accumulation in alcoholic liver disease.

J Lipid Res 2019 Feb 21. Epub 2019 Feb 21.

Huazhong University of Science and Technology, China

Sensitization hepatic immune cells of chronic alcoholic consumption gives rise to inflammatory accumulation which is considered as leading cause to liver damage. T regulatory cells (Tregs) are immunosuppressive cell subset that play an important role in a variety of liver diseases, however, data about pathological involvement of Tregs in liver steatosis of ALD is insufficient. In mouse models of ALD, we found that increased lipid accumulation by chronic alcohol intake as companioned with oxidative stress, inflammatory accumulation as well as Treg decline in the liver. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1194/jlr.M083568DOI Listing
February 2019

Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats.

Neuron 2019 Feb 8. Epub 2019 Feb 8.

Molecular Mechanisms of Cellular Stress and Inflammation Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA; Optogenetics and Transgenic Technology Core/Genetic Engineering and Viral Vector Core, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. Electronic address:

Historically, the rat has been the preferred animal model for behavioral studies. Limitations in genome modification have, however, caused a lag in their use compared to the bevy of available transgenic mice. Here, we have developed several transgenic tools, including viral vectors and transgenic rats, for targeted genome modification in specific adult rat neurons using CRISPR-Cas9 technology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuron.2019.01.035DOI Listing
February 2019

Wound healing activity of terpinolene and α-phellandrene by attenuating inflammation and oxidative stress in vitro.

J Tissue Viability 2019 Feb 14. Epub 2019 Feb 14.

Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Produtos Naturais, Universidade Vila Velha, CEP 29102-920, Brazil. Electronic address:

This study was undertaken to investigate the in vitro wound healing effects and the anti-inflammatory and antioxidant activities of terpinolene and α-phellandrene. The in vitro stimulatory effects on the proliferation and migration of fibroblasts were assessed using the scratch assay. The anti-inflammatory activity was evaluated using cell-based assays by investigating their influence on nitric oxide (NO), superoxide anion (O), tumour necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) production and using the TNF-α-induced nuclear factor kappa (NF-κB) assay. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtv.2019.02.003DOI Listing
February 2019

JNK1/β-catenin axis regulates HO-induced epithelial-to-mesenchymal transition in human lens epithelial cells.

Biochem Biophys Res Commun 2019 Feb 18. Epub 2019 Feb 18.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, People's Republic of China.

Epithelial-mesenchymal transition (EMT) is the main cause of fibrotic cataracts. Oxidative stress was recently shown to trigger epithelial-mesenchymal transition in human lens epithelial cells (hLECs). However, the underlying mechanism is not fully understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2019.02.049DOI Listing
February 2019

Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.

Genome Biol 2019 Feb 21;20(1):37. Epub 2019 Feb 21.

Department of Pathology and Laboratory Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA.

Background: Protein recoding by RNA editing is required for normal health and evolutionary adaptation. However, de novo induction of RNA editing in response to environmental factors is an uncommon phenomenon. While APOBEC3A edits many mRNAs in monocytes and macrophages in response to hypoxia and interferons, the physiological significance of such editing is unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13059-019-1651-1DOI Listing
February 2019

Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells.

BMC Complement Altern Med 2019 Feb 21;19(1):50. Epub 2019 Feb 21.

NICM Health Research Institute, Western Sydney University, Sydney, 2751, Australia.

Background: This study investigated the protective effects of the Danshen (DS) and Sanqi (SQ) herb pair on cell survival in the human cardiovascular endothelial (EA.hy926) cell line exposed to injury.

Methods: Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their protective effects in the EA. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12906-019-2458-zDOI Listing
February 2019

Proteome profiling of triple negative breast cancer cells overexpressing NOD1 and NOD2 receptors unveils molecular signatures of malignant cell proliferation.

BMC Genomics 2019 Feb 21;20(1):152. Epub 2019 Feb 21.

SBP Medical Discovery Institute, 10901 North Torrey Pines Rd, La Jolla, CA, 92037, USA.

Background: Triple negative breast cancer (TNBC) is a malignancy with very poor prognosis, due to its aggressive clinical characteristics and lack of response to receptor-targeted drug therapy. In TNBC, immune-related pathways are typically upregulated and may be associated with a better prognosis of the disease, encouraging the pursuit for immunotherapeutic options. A number of immune-related molecules have already been associated to the onset and progression of breast cancer, including NOD1 and NOD2, innate immune receptors of bacterial-derived components which activate pro-inflammatory and survival pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-019-5523-6DOI Listing
February 2019

Genome-Wide Analysis of Auxin Receptor Family Genes in var. .

Genes (Basel) 2019 Feb 20;10(2). Epub 2019 Feb 20.

College of Life Science and Technology, Yangtze Normal University, Chongqing 408100, China.

Transport inhibitor response 1/auxin signaling f-box proteins TIR1/AFBs) play important roles in the process of plant growth and development as auxin receptors. To date, no information has been available about the characteristics of the gene family in var. . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes10020165DOI Listing
February 2019

Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium-Role in Dead Cell Clearance and Inflammation.

Int J Mol Sci 2019 Feb 20;20(4). Epub 2019 Feb 20.

Department of Biochemistry and Molecular Biology, University of Debrecen, Faculty of Medicine, 4032 Debrecen, Hungary.

Inefficient removal of dying retinal pigment epithelial (RPE) cells by professional phagocytes can result in debris formation and development of age-related macular degeneration (AMD). Chronic oxidative stress and inflammation play an important role in AMD pathogenesis. Only a few well-established in vitro phagocytosis assay models exist. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms20040926DOI Listing
February 2019

IRES Trans-Acting Factors, Key Actors of the Stress Response.

Int J Mol Sci 2019 Feb 20;20(4). Epub 2019 Feb 20.

UMR 1048-I2MC, Inserm, Université de Toulouse, UT3, 31432 Toulouse cedex 4, France.

The cellular stress response corresponds to the molecular changes that a cell undergoes in response to various environmental stimuli. It induces drastic changes in the regulation of gene expression at transcriptional and posttranscriptional levels. Actually, translation is strongly affected with a blockade of the classical cap-dependent mechanism, whereas alternative mechanisms are activated to support the translation of specific mRNAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms20040924DOI Listing
February 2019

Thioredoxin Reductase Activity Predicts Gold Nanoparticle Radiosensitization Effect.

Nanomaterials (Basel) 2019 Feb 19;9(2). Epub 2019 Feb 19.

Research Center for the Physics of Matter and Radiation (PMR-LARN), Namur Research Institute for Life Sciences (NARILIS), University of Namur, Rue de Bruxelles 61, B-5000 Namur, Belgium.

Gold nanoparticles (GNPs) have been shown to be effective contrast agents for imaging and emerge as powerful radiosensitizers, constituting a promising theranostic agent for cancer. Although the radiosensitization effect was initially attributed to a physical mechanism, an increasing number of studies challenge this mechanistic hypothesis and evidence the importance of oxidative stress in this process. This work evidences the central role played by thioredoxin reductase (TrxR) in the GNP-induced radiosensitization. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano9020295DOI Listing
February 2019

Overexpression of Transglutaminase from Cucumber in Tobacco Increases Salt Tolerance through Regulation of Photosynthesis.

Int J Mol Sci 2019 Feb 19;20(4). Epub 2019 Feb 19.

Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.

Transglutaminase (TGase) is a regulator of posttranslational modification of protein that provides physiological protection against diverse environmental stresses in plants. Nonetheless, the mechanisms of TGase-mediated salt tolerance remain largely unknown. Here, we found that the transcription of cucumber () was induced in response to light and during leaf development, and the CsTGase protein was expressed in the chloroplast and the cell wall. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms20040894DOI Listing
February 2019

Lessons from the Discovery of Mitochondrial Fragmentation (Fission): A Review and Update.

Cells 2019 Feb 19;8(2). Epub 2019 Feb 19.

A.N.Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119991, Russia.

Thirty-five years ago, we described fragmentation of the mitochondrial population in a living cell into small vesicles (mitochondrial fission). Subsequently, this phenomenon has become an object of general interest due to its involvement in the process of oxidative stress-related cell death and having high relevance to the incidence of a pathological phenotype. Tentatively, the key component of mitochondrial fission process is segregation and further asymmetric separation of a mitochondrial body yielding healthy (normally functioning) and impaired (incapable to function in a normal way) organelles with subsequent decomposition and removal of impaired elements through autophagy (mitophagy). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells8020175DOI Listing
February 2019

Transcriptome sequencing of Antarctic moss under salt stress emphasizes the important roles of the ROS-scavenging system.

Gene 2019 Feb 18. Epub 2019 Feb 18.

School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, Shangdong, China.

Mosses are predominant terrestrial vegetation in Antarctica. Their distributions appear to be controlled more by water and salinity. The Antarctic moss Pohlia nutans can tolerate high salt stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2019.02.037DOI Listing
February 2019

Doping of carbon nanodots for saving cells from silver nanotoxicity: A study on recovering osteogenic differentiation potential.

Toxicol In Vitro 2019 Feb 18. Epub 2019 Feb 18.

School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India. Electronic address:

Silver nanoparticles are explored for many advanced biological applications including the development of antimicrobial surfaces on implants, SERS imaging, nanotherapeutics, biosensing and much more. However, recent research findings suggest silver nanoparticles provide blockade of differentiation of mesenchymal stem cells (MSCs), especially into osteogenic developmental pathway via generation of reactive oxygen species. These studies suggest that the application of silver nanoparticles in medical implants should be prohibited. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tiv.2019.02.015DOI Listing
February 2019

Cordycepin mitigates MPTP-induced Parkinson's disease through inhibiting TLR/NF-κB signaling pathway.

Life Sci 2019 Feb 18. Epub 2019 Feb 18.

Department of Neurology, Heze Municiple Hospital, Heze, Shandong, China. Electronic address:

Parkinson's disease (PD) is a neurodegenerative disease with movement disorder. PD is characterized by the loss of dopaminergic (DA) neurons in the substantia nigra. Cordycepin, a small molecule extracted from cordyceps sinensis, has neuroprotective, anti-inflammatory, antioxidant and anti-tumor properties. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2019.02.037DOI Listing
February 2019

Transcriptional Regulation of Nos2 via STAT5B Binding to Nos2 Gene Promoter Mediates Nitric Oxide Production: Relevance in β-Cell Maintenance.

Cell Physiol Biochem 2019 18;52(1):141-155. Epub 2019 Feb 18.

Female Reproduction and Metabolic Syndromes Laboratory, Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.

Background/aims: Type 1 Diabetes (T1D) involves autoimmune attack due to reduced regulatory T cells as an effect of mutant Stat5b(C1462A) in non-obese diabetic (NOD) mice, a T1D model resulting in pancreatic β-cell destruction. Although reactive oxygen species are considered to orchestrate the immune attack, the role of nitric oxide (·NO) still remains debatable. Since JAK-STAT pathway is known to induce Nos2, we investigated the role of STAT5B in nitric oxide generation and oxidative stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000010DOI Listing
February 2019

Non-Thermal Plasma, as a New Physicochemical Source, to Induce Redox Imbalance and Subsequent Cell Death in Liver Cancer Cell Lines.

Cell Physiol Biochem 2019 18;52(1):119-140. Epub 2019 Feb 18.

Institute of Physics of the Czech Academy of Sciences, Prague, Czech Republic.

Background/aims: Alteration of cancer cell redox status has been recognized as a promising therapeutic implication. In recent years, the emerged field of non-thermal plasma (NTP) has shown considerable promise in various biomedical applications, including cancer therapy. However, understanding the molecular mechanisms procuring cellular responses remains incomplete. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33594/000000009DOI Listing
February 2019