208,606 results match your criteria Cell Stress & Chaperones [Journal]


Theracurmin Ameliorates Cognitive Dysfunctions in 5XFAD Mice by Improving Synaptic Function and Mitigating Oxidative Stress.

Biomol Ther (Seoul) 2019 Apr 19. Epub 2019 Apr 19.

Departments of Life and Nanopharmaceutical Science, Kyung Hee University, Seoul 02447, Republic of Korea.

As the elderly population is increasing, Alzheimer’s disease (AD) has become a global issue and many clinical trials have been conducted to evaluate treatments for AD. As these clinical trials have been conducted and have failed, the development of new theraphies for AD with fewer adverse effects remains a challenge. In this study, we examined the effects of Theracurmin on cognitive decline using 5XFAD mice, an AD mouse model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4062/biomolther.2019.046DOI Listing

Blueberry juice protects osteocytes and bone precursor cells against oxidative stress partly through SIRT1.

FEBS Open Bio 2019 Apr 1. Epub 2019 Apr 1.

Department of Biomedical Experimental and Clinical Sciences 'Mario Serio', University of Florence, Italy.

Oxidative stress and abnormal osteocyte apoptosis are often related to dysregulation of bone turnover and chronic bone loss, and so fruit and vegetables with high antioxidant potential may play an important role in the prevention and/or management of osteoporosis. Osteocytes are the main regulators of bone remodelling. For the first time, we demonstrate here that blueberry juice (BJ), obtained from Vaccinium myrtillus, rich in polyphenols, shows antioxidant and antiosteoclastogenic properties in MLO-Y4 osteocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/2211-5463.12634DOI Listing

MaizeNet: A co-functional network for network-assisted systems genetics in Zea mays.

Plant J 2019 Apr 21. Epub 2019 Apr 21.

Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, 03722, Korea.

Maize (Zea mays) has multiple uses in human food, animal fodder, starch and sweetener production, and as a biofuel, and is accordingly the most extensively cultivated cereal worldwide. To enhance maize production, genetic factors underlying important agricultural traits, including stress tolerance and flowering, have been explored through forward and reverse genetics approaches. Co-functional gene networks are systems biology resources useful in identifying trait-associated genes in plants by prioritizing candidate genes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/tpj.14341DOI Listing

Lycopene protects against t-BHP-induced neuronal oxidative damage and apoptosis via activation of the PI3K/Akt pathway.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Department of Pathophysiology, Institute of Brain Science Research, Key Laboratory of State Administration of Traditional Chinese Medicine of the People's Republic of China, School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China.

Oxidative stress is a key factor of and closely implicated in the pathogenesis of Alzheimer's disease (AD). We herein used tert-butyl hydroperoxide (t-BHP) to induce oxidative stress and mimic oxidative neurotoxicity in vitro. Lycopene is a natural antioxidant that has a strong ability to eliminate free radicals and shows effective protection in some neurodegenerative disease models. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s11033-019-04801-y
Publisher Site
http://dx.doi.org/10.1007/s11033-019-04801-yDOI Listing
April 2019
1 Read

Cyclic tensile stress promotes osteogenic differentiation of adipose stem cells via ERK and p38 pathways.

Stem Cell Res 2019 Apr 8;37:101433. Epub 2019 Apr 8.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address:

The present study aimed to elucidate whether extracellular signal-regulated kinases 1/2 (ERK1/2) and p38 mitogen-activated protein kinases pathways participate in the transduction of mechanical stretch exerted on adipose stem cells (ASCs) into intracellular osteogenic signals, and if so whether both pathways have time-dependent feature. Rat ASCs were cultured in osteogenic medium for 72 h and assigned into three sets, namely ERK1/2 inhibitor treated set, p38 inhibitor treated set, and the control set. After inhibitor treatment, all cells were subjected to cyclic stretch(2000 με, 1 Hz) on a four-point bending mechanical loading device. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2019.101433DOI Listing

Superoxide dismutase in nanoarchaeosomes for targeted delivery to inflammatory macrophages.

Colloids Surf B Biointerfaces 2019 Mar 28;179:479-487. Epub 2019 Mar 28.

Nanomedicine Research & Development Center, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD, Argentina. Electronic address:

Oxidative stress plays an essential role in the pathogenesis and progression of inflammatory bowel disease. Co-administration of antioxidants and anti-inflammatory drugs has shown clinical benefits. Due to its significant reactive oxygen species (ROS) scavenging ability, great interest has been focused on superoxide dismutase (SOD) for therapeutic use. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S09277765193021
Publisher Site
http://dx.doi.org/10.1016/j.colsurfb.2019.03.061DOI Listing
March 2019
1 Read

Variations of salivary concentration of cytokines and chemokines in presence of oral squamous cell carcinoma. A case-crossover longitudinal prospective study.

Cytokine 2019 Apr 18;120:62-65. Epub 2019 Apr 18.

University of Turin, Department of Oncology, Unit of Oral Medicine and Oral Oncology, Italy.

Salivary biomarkers represent a promising diagnostic tool. Non-invasive and stress-free sampling results in objective parameters able to overcome the need of clinical expertise required for a correct interpretation of early oral squamous cell carcinoma (OSCC). The present study aims at evaluating potential variations in the salivary concentration of cytokines in presence of OSCC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cyto.2019.04.009DOI Listing

(-)-Epigallocatechin-3-gallate suppresses cigarette smoke-induced inflammation in human cardiomyocytes via ROS-mediated MAPK and NF-κB pathways.

Phytomedicine 2018 Nov 20;58:152768. Epub 2018 Nov 20.

Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong; Department of Pharmacology & Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong. Electronic address:

Background: Cigarette smoking is the leading cause for the initiation and development of cardiovascular disease (CVD). Oxidative stress and inflammatory responses play important roles in the pathophysiological processes of smoking-induced cardiac injury. (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin in green tea, which is made from Camellia sinensis leaves, has been reported to possess potent anti-oxidant property. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2018.11.028DOI Listing
November 2018

Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway.

Toxicology 2019 Apr 18. Epub 2019 Apr 18.

Department of Pharmacy, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address:

Myocarditis is a serious hazard to human life and is difficult to treat due to the proliferation of inflammatory lesions in the myocardium. Leonurine (LE) is a plant phenolic alkaloid extracted from Herba leonuri that has demonstrated cardioprotective effects in many preclinical experiments. However, whether LE can be used for myocarditis therapy has not been reported. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tox.2019.04.011DOI Listing

Eco-electrogenic treatment of dyestuff wastewater using constructed wetland-microbial fuel cell system with an evaluation of electrode-enriched microbial community structures.

Bioresour Technol 2019 Apr 13;285:121349. Epub 2019 Apr 13.

P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology CHARUSAT Campus, Changa - 388421, Gujarat, India. Electronic address:

This work studied eco-electrogenic treatment of real dyestuff wastewater along with characterization of electrode-enriched microbial community structures in Fimbristylis dichotoma planted closed-circuit constructed wetland-microbial fuel cell (CW-MFC) system. The CW-MFC-2 (experimental system) achieved 82.2 ± 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.121349DOI Listing

Protective effect of dietary vitamin E on immunological and biochemical induction through silver nanoparticles (AgNPs) inclusion in diet and silver salt (AgNO) exposure on Zebrafish (Danio rerio).

Comp Biochem Physiol C Toxicol Pharmacol 2019 Apr 17. Epub 2019 Apr 17.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Science and Technology Research Institute, Chiang Mai University, 239 Huay keaw Rd., Suthep, Muang, Chiang Mai 50200, Thailand. Electronic address:

The present study evaluated silver nanoparticle (AgNPs) toxicity using biomarkers of oxidative and metabolic stress, immunological impairment and cellular damage in zebrafish (Danio rerio), as well as the optimal dose of vitamin E neutralizing undesirable effects. Fish were fed for ten days and eight study groups were investigated: controls, AgNPs exposure alone (1.5 mg L) and combined with three different vitamin E doses (1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpc.2019.04.004DOI Listing

Mechanism of isoniazid-induced hepatotoxicity in zebrafish larvae: Activation of ROS-mediated ERS, apoptosis and the Nrf2 pathway.

Chemosphere 2019 Apr 6;227:541-550. Epub 2019 Apr 6.

Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Key Laboratory for Biosensor of Shandong Province, Jinan, Shandong Province, PR China. Electronic address:

Isoniazid (INH) is a first-line anti-tuberculosis drug. INH has been detected in surface waters which may create a risk to aquatic organisms. In this study, the hepatotoxicity of INH was elucidated using zebrafish. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00456535193067
Publisher Site
http://dx.doi.org/10.1016/j.chemosphere.2019.04.026DOI Listing
April 2019
1 Read

Protective function of autophagy during VLCFA-induced cytotoxicity in a neurodegenerative cell model.

Free Radic Biol Med 2019 Apr 17. Epub 2019 Apr 17.

Université de Bourgogne Franche-Comté, Dijon, F-21000, France; Team 'Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism' EA 7270, Inserm, F-21000, Dijon, France. Electronic address:

In recent years, a particular interest has focused on the accumulation of fatty acids with very long chains (VLCFA) in the occurrence of neurodegenerative diseases such as Alzheimer's disease, multiple sclerosis or dementia. Indeed, it seems increasingly clear that this accumulation of VLCFA in the central nervous system is accompanied by a progressive demyelination resulting in death of neuronal cells. Nevertheless, molecular mechanisms by which VLCFA result in toxicity remain unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.freeradbiomed.2019.04.016DOI Listing

Chlorogenic acid promotes the Nrf2/HO-1 anti-oxidative pathway by activating p21 to resist dexamethasone-induced apoptosis in osteoblastic cells.

Free Radic Biol Med 2019 Apr 17. Epub 2019 Apr 17.

College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China. Electronic address:

In a previous study, p21 (p21) promoted activation of the nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway, which has an important role in regulating apoptosis triggered by oxidative stress and inhibiting development of osteoporosis. Chlorogenic acid (CGA) has a strong protective effect on osteoporosis, closely related to activating the Nrf2/HO-1 pathway. However, whether CGA can resist apoptosis by regulating p21 and consequently promote activation of the Nrf2/HO-1 pathway needs further investigation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.freeradbiomed.2019.04.014DOI Listing

Dual role of autophagy/mitophagy in chronic obstructive pulmonary disease.

Pulm Pharmacol Ther 2019 Apr 17. Epub 2019 Apr 17.

Department of Integrated Traditional Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai, China. Electronic address:

Chronic obstructive pulmonary disease (COPD) is a common lung disease characterised by progressive, irreversible airflow limitation. Multiple regulatory pathways are involved in COPD pathogenesis. Emerging evidence from clinical and basic medical research has suggested that autophagy-a highly conserved catabolic process mediated under various cellular stress conditions-plays a role in the development and prognosis of COPD. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S10945539183024
Publisher Site
http://dx.doi.org/10.1016/j.pupt.2019.04.002DOI Listing
April 2019
1 Read

Alumina nanoparticles size and crystalline phase impact on cytotoxic effect on alveolar epithelial cells after simple or HCl combined exposures.

Toxicol In Vitro 2019 Apr 17. Epub 2019 Apr 17.

Institut de Recherche Biomédicale des Armées (IRBA), Département EBR, Unité des Risques Technologiques Emergents, BP 73, 91223, Brétigny-sur-Orge Cedex, France. Electronic address:

Applications using alumina nanoparticles (AlO NPs) have incredibly increased in different fields of activity. In defense and aerospace fields, solid composite propellants use leads to complex combustion aerosols emissions containing high concentrations of AlO NPs and hydrogen chloride gas (HCl). To better characterize potential hazard resulting from exposure to these aerosols, this study assesses cytotoxic effects of mixtures containing both compounds on human pulmonary alveolar epithelial cells (A549 cell line) after 24 h exposures. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tiv.2019.04.016DOI Listing

Anti-cancer effects of polyphenols via targeting p53 signaling pathway: updates and future directions.

Biotechnol Adv 2019 Apr 17. Epub 2019 Apr 17.

Institute of Chinese Medical Sciences, State Key Laboratory of Quality Control in Chinese Medicine, University of Macau, Macau. Electronic address:

The anticancer effects of polyphenols are ascribed to several signaling pathways including the tumor suppressor gene tumor protein 53 (p53). Expression of endogenous p53 is silent in various types of cancers. A number of polyphenols from a wide variety of dietary sources could upregulate p53 expression in several cancer cell lines through distinct mechanisms of action. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biotechadv.2019.04.007DOI Listing

The relationship between inflammasomes and the endoplasmic reticulum stress response in the injured spinal cord.

Neurosci Lett 2019 Apr 17. Epub 2019 Apr 17.

Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine Isehara, Japan. Electronic address:

Study Design animal study Objectives The aim of this study is to investigate the influence of inflammasomes in the injured spinal cord of a rat spinal cord injury model. Setting University laboratory in Kanagawa, Japan Methods A thoracic contusion spinal cord injury (SCI) was induced in female Sprague Dawley rats using an IH-impactor to create a moderate injury group (LI) and a severe injury group (HI). Using a sham group as a control, the injured spinal cords were removed at several time points after injury to evaluate the levels of inflammasome component proteins in the injured spinal cord by immunohistochemistry and Western Blot. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neulet.2019.04.033DOI Listing

Analysis of the cytotoxic, genotoxic, mutagenic, and pro-oxidant effect of synephrine, a component of thermogenic supplements, in human hepatic cells in vitro.

Toxicology 2019 Apr 17. Epub 2019 Apr 17.

Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/n°, 14040-903, Ribeirão Preto, São Paulo, Brazil. Electronic address:

Thermogenic supplements containing synephrine (SN) are widely used to weight loss. SN is a proto-alkaloid naturally found in the bark of immature fruits of Citrus aurantium (bitter orange) that has been added to thermogenic supplements due to its chemical and pharmacological similarity with adrenergic amines, such as ephedrine and amphetamines. Although orally ingested SN is mainly metabolized in the liver, it remains unclear whether it affects the redox status and genetic material of human hepatic cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tox.2019.04.010DOI Listing

Heparanase protects the heart against chemical or ischemia/reperfusion injury.

J Mol Cell Cardiol 2019 Apr 17. Epub 2019 Apr 17.

Faculty of Pharmaceutical Sciences, University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC V6T 1Z3, Canada. Electronic address:

Although cancer cells use heparanase for tumor metastasis, favourable effects of heparanase have been reported in the management of Alzheimer's disease and diabetes. Indeed, we previously established a protective function for heparanase in the acutely diabetic heart, where it conferred cardiomyocyte resistance to oxidative stress and apoptosis by provoking changes in gene expression. In this study, we tested if overexpression of heparanase can protect the heart against chemically induced or ischemia/reperfusion (I/R) injury. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yjmcc.2019.04.008DOI Listing

NPC1-deficient neurons are selectively vulnerable for statin treatment.

Neuropharmacology 2019 Apr 17. Epub 2019 Apr 17.

Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin und Berlin Institute of Health; Institut für Integrative Neuroanatomie, Klinische Zell- und Neurobiologie, CC2, Charité, 10117, Berlin, Germany.

Niemann Pick C (NPC) is a fatal hereditary neurovisceral disorder associated with a progressive loss of neurons of unknown mechanism. The disease is caused by mutation in either of two genes, termed npc1 and npc2, accounting for ∼95% and ∼5% of patients, respectively. Recent data suggest a cell-autonomous cause for neuronal cell death. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuropharm.2019.04.012DOI Listing
April 2019
1 Read

REST: An epigenetic regulator of neuronal stress responses in the young and ageing brain.

Front Neuroendocrinol 2019 Apr 17. Epub 2019 Apr 17.

Neuroimmunology & Neurotherapeutics Laboratory, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UK. Electronic address:

The transcriptional repressor REST (Repressor Element-1 Silencing Transcription factor) is a key modulator of the neuronal epigenome and targets genes involved in neuronal differentiation, axonal growth, vesicular transport, ion channel conductance and synaptic plasticity. Whilst its gene expression-modifying properties have been examined extensively in neuronal development, REST's response towards stress-induced neuronal insults has only recently been explored. Overall, REST appears to be an ideal candidate to fine-tune neuronal gene expression following different forms of cellular, neuropathological, psychological and physical stressors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yfrne.2019.04.001DOI Listing

Long non-coding RNA-p21 regulates MPP-induced neuronal injury by targeting miR-625 and derepressing TRPM2 in SH-SY5Y cells.

Chem Biol Interact 2019 Apr 17. Epub 2019 Apr 17.

Department of Blood Transfusion, Central Hospital of Linyi, No. 17, Health Road, Yishui County, 276400, Linyi City, Shandong Province, China. Electronic address:

Parkinson's disease (PD), the second most prevalent age-related neurodegenerative disease, occurs as a result of the loss of dopaminergic neurons in the substantia nigra. Long non-coding RNA-p21 (lnc-p21) has been demonstrated to be upregulated in PD. However, its role in PD is unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbi.2019.04.017DOI Listing

The lipid flippase subunit Cdc50 is required for antifungal drug resistance, endocytosis, hyphal development and virulence in Candida albicans.

FEMS Yeast Res 2019 Apr 20. Epub 2019 Apr 20.

College of Life Sciences, Huaibei Normal University, Huaibei 235000, Anhui, China.

Cdc50 is the non-catalytic subunit of the flippase that establishes phospholipid asymmetry in membranes and functions in vesicle-mediated trafficking in Saccharomyces cerevisiae. Here, we have identified the homologous gene CaCDC50 that encodes a protein of 396 amino acids with two conserved transmembrane domains in Candida albicans. Deletion of CaCDC50 results in C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/femsyr/foz033DOI Listing

Prolactin Receptor Signaling Regulates a Pregnancy-Specific Transcriptional Program in Mouse Islets.

Endocrinology 2019 May;160(5):1150-1163

Division of Endocrinology, Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.

Pancreatic β-cells undergo profound hyperplasia during pregnancy to maintain maternal euglycemia. Failure to reprogram β-cells into a more replicative state has been found to underlie susceptibility to gestational diabetes mellitus (GDM). We recently identified a requirement for prolactin receptor (PRLR) signaling in the metabolic adaptations to pregnancy, where β-cell-specific PRLR knockout (βPRLRKO) mice exhibit a metabolic phenotype consistent with GDM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1210/en.2018-00991DOI Listing

Circular RNA MAT2B promotes glycolysis and malignancy of hepatocellular carcinoma via the miR-338-3p/PKM2 axis under hypoxic stress.

Hepatology 2019 Apr 20. Epub 2019 Apr 20.

School of Medicine, Southeast University, No.87, Dingjia Bridge, Nanjing, Jiangsu province, China.

Glucose metabolism reprogramming, which is a well-established characteristic of multiple cancers, demands a higher rate of glycolysis to meet the increasing demands for macromolecular synthesis and to maintain rapid proliferation in a hypoxic environment. However, the mechanism underlying this switch remains to be elucidated. In this study, we investigated the function of circular RNA MAT2B (circMAT2B) in hepatocellular carcinoma (HCC) glucose metabolism reprogramming and malignancy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/hep.30671DOI Listing

The role of inflammation, oxidative stress, angiogenesis, and apoptosis in the pathophysiology of endometriosis: Basic science and new insights based on gene expression.

J Cell Physiol 2019 Apr 19. Epub 2019 Apr 19.

Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.

Endometriosis is a frequent and chronic illness in young women which could be defined by the existence of endometrial stroma and glands outside of the normal site of the lining of the uterus. It has painful symptoms. The advanced stage of endometriosis may lead to gynecological malignancies, such as ovarian cancer, and other complications, including infertility. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcp.28666DOI Listing

Autologous Muscle-derived Cell Injection for Treatment of Female Stress Urinary Incontinence: A Single-Arm Clinical Trial with 24-months Follow-Up.

Urol J 2019 Apr 20. Epub 2019 Apr 20.

Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

Introduction: This clinical study evaluated the effect of autologous muscle-derived cell (MDC) injection for the treatment of female patients with pure stress urinary incontinence (SUI).

Materials And Methods: A total of 20 women with SUI received transurethral injections of autologous MDCs. Baseline and follow-up evaluations consisted of physical examinations (cough stress tests), one-hour pad test, Incontinence Impact Questionnaire-7 (IIQ-7), and Urogenital Distress Inventory (UDI-6) scoring. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.22037/uj.v0i0.4736DOI Listing

MicroRNA-132-3p represses Smad5 in MC3T3-E1 osteoblastic cells under cyclic tensile stress.

Mol Cell Biochem 2019 Apr 19. Epub 2019 Apr 19.

School of Basic Medical Science, Shanxi Medical University, 56 Xinjian Road, Ying Ze, Taiyuan, 030001, China.

MicroRNAs (miRNAs) regulate osteogenic differentiation of bone cells, which has applications in orthodontics. Here we evaluated the miRNA expression profile of MC3T3-E1 osteoblasts under cyclic tensile stress with chip technology and found that miR-132-3p was up-regulated by 12% cyclic tensile stress. Alkaline phosphatase activity and osteocalcin expression in MC3T3-E1 cells were decreased under these conditions. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s11010-019-03538-3
Publisher Site
http://dx.doi.org/10.1007/s11010-019-03538-3DOI Listing
April 2019
1 Read

Nanoliposome-encapsulated ellagic acid prevents cyclophosphamide-induced rat liver damage.

Mol Cell Biochem 2019 Apr 19. Epub 2019 Apr 19.

Department of Physiology, Faculty of Medicine, University of Nis, Bulevar Zorana Ðinđića 81, Niš, 18000, Serbia.

In this study, we aimed to evaluate whether the encapsulation of ellagic acid (EA) into nanoliposomes would improve its potential in preventing cyclophosphamide-induced liver damage. Stability and antioxidative potential of free and encapsulated EA were determined. Experimental study conducted in vivo included ten groups of rats treated with cyclophosphamide and ellagic acid in its free and encapsulated form during 5 days. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s11010-019-03541-8
Publisher Site
http://dx.doi.org/10.1007/s11010-019-03541-8DOI Listing
April 2019
1 Read

Identification of sugar response complex in the metallothionein OsMT2b gene promoter for enhancement of foreign protein production in transgenic rice.

Plant Cell Rep 2019 Apr 19. Epub 2019 Apr 19.

Department of BioAgricultural Sciences, National Chiayi University, Chiayi, 60004, Taiwan.

Key Message: A 146-bp sugar response complex MTSRC is identified in the promoter of rice metallothionein OsMT2b gene conferring high-level expression of luciferase reporter gene and bioactive recombinant haFGF in transgenic rice. A rice subfamily type 2 plant metallothionein (pMT) gene, OsMT2b, encoding a reactive oxygen species (ROS) scavenger protein, has been previously shown to exhibit the most abundant gene expression in young rice seedling. Expression of OsMT2b was found to be regulated negatively by ethylene and hydrogen peroxide in rice stem node under flooding stress, but little is known about its response to sugar depletion. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00299-019-02411-3DOI Listing

Alarmone Ap4A is elevated by aminoglycoside antibiotics and enhances their bactericidal activity.

Proc Natl Acad Sci U S A 2019 Apr 19. Epub 2019 Apr 19.

Faculty of Health Sciences, University of Macau, Macau SAR, China;

Second messenger molecules play important roles in the responses to various stimuli that can determine a cell's fate under stress conditions. Here, we report that lethal concentrations of aminoglycoside antibiotics result in the production of a dinucleotide alarmone metabolite-diadenosine tetraphosphate (Ap4A), which promotes bacterial cell killing by this class of antibiotics. We show that the treatment of with lethal concentrations of kanamycin (Kan) dramatically increases the production of Ap4A. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.1822026116DOI Listing
April 2019
2 Reads

Ttc7a regulates haematopoietic stem cell functions while controlling the stress-induced response.

Haematologica 2019 Apr 19. Epub 2019 Apr 19.

Laboratory of Normal and Pathological Homeostasis of the Immune System, Paris, France

The molecular machinery that regulates the balance between self-renewal and differentiation properties of hematopoietic stem cells has yet to be characterized in detail. Here we found that the tetratricopeptide repeat domain 7 A (Ttc7a) protein, a putative scaffold protein expressed by hematopoietic stem cells, acts as an intrinsic regulator of the proliferative response and the self- renewal potential of murine hematopoietic stem cells in vivo. Loss of Ttc7a consistently enhanced the hematopoietic stem cells' competitive repopulation ability and their intrinsic capacity to replenish the hematopoietic system after serial cell transplantations, relative to wild-type cells. Read More

View Article

Download full-text PDF

Source
http://www.haematologica.org/lookup/doi/10.3324/haematol.201
Publisher Site
http://dx.doi.org/10.3324/haematol.2018.207100DOI Listing
April 2019
1 Read

M Muscarinic Receptors Modulate Fear-Related Inputs to the Prefrontal Cortex: Implications for Novel Treatments of Posttraumatic Stress Disorder.

Biol Psychiatry 2019 Mar 7. Epub 2019 Mar 7.

Department of Pharmacology, Vanderbilt University, Nashville, Tennessee; Vanderbilt Brain Institute, Vanderbilt University, Nashville, Tennessee; Vanderbilt Kennedy Center, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee; Vanderbilt Center for Neuroscience Drug Discovery, Vanderbilt University, Nashville, Tennessee. Electronic address:

Background: The prefrontal cortex (PFC) integrates information from multiple inputs to exert top-down control allowing for appropriate responses in a given context. In psychiatric disorders such as posttraumatic stress disorder, PFC hyperactivity is associated with inappropriate fear in safe situations. We previously reported a form of muscarinic acetylcholine receptor (mAChR)-dependent long-term depression in the PFC that we hypothesize is involved in appropriate fear responding and could serve to reduce cortical hyperactivity following stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopsych.2019.02.020DOI Listing

MicroRNA-146b-5p protects oligodendrocyte precursor cells from oxygen/glucose deprivation-induced injury through regulating Keap1/Nrf2 signaling via targeting bromodomain-containing protein 4.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Institute of Neurobiology, The Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China.

The injury of oligodendrocyte precursor cells (OPCs) contributes to the pathology of hypoxic-ischemic encephalopathy in newborns. MicroRNAs (miRNAs) have emerge as critical regulators of hypoxic-ischemic encephalopathy; however, the role of miRNAs in regulating OPC injury remains largely unknown. MiRNA-146b-5p (miR-146b-5p) has been reported to exert a cytoprotective function under various pathological conditions. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X193067
Publisher Site
http://dx.doi.org/10.1016/j.bbrc.2019.04.045DOI Listing
April 2019
1 Read

Quantitative and comparative analysis of whole-plant performance for functional physiological traits phenotyping: New tools to support pre-breeding and plant stress physiology studies.

Plant Sci 2019 May 17;282:49-59. Epub 2018 May 17.

The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 7610001, Israel. Electronic address:

Plants are autotrophic organisms in which there are linear relationships between the rate at which organic biomass is accumulated and many ambient parameters such as water, nutrients, CO and solar radiation. These linear relationships are the result of good feedback regulation between a plants sensing of the environment and the optimization of its performance response. In this review, we suggest that continuous monitoring of the plant physiological profile in response to changing ambient conditions could be a useful new phenotyping tool, allowing the characterization and comparison of different levels of functional phenotypes and productivity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plantsci.2018.05.008DOI Listing

The Cytoskeleton-A Complex Interacting Meshwork.

Cells 2019 Apr 18;8(4). Epub 2019 Apr 18.

Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle (Saale), Germany.

The cytoskeleton of animal cells is one of the most complicated and functionally versatile structures, involved in processes such as endocytosis, cell division, intra-cellular transport, motility, force transmission, reaction to external forces, adhesion and preservation, and adaptation of cell shape. These functions are mediated by three classical cytoskeletal filament types, as follows: Actin, microtubules, and intermediate filaments. The named filaments form a network that is highly structured and dynamic, responding to external and internal cues with a quick reorganization that is orchestrated on the time scale of minutes and has to be tightly regulated. Read More

View Article

Download full-text PDF

Source
https://www.mdpi.com/2073-4409/8/4/362
Publisher Site
http://dx.doi.org/10.3390/cells8040362DOI Listing
April 2019
1 Read

A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein.

Sensors (Basel) 2019 Apr 18;19(8). Epub 2019 Apr 18.

Department of Environmental Health Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea.

Microbial cell-based biosensors, which mostly rely on stress-responsive operons, have been widely developed to monitor environmental pollutants. Biosensors are usually more convenient and inexpensive than traditional instrumental analyses of environmental pollutants. However, the targets of biosensors are restricted by the limited number of genetic operon systems available. Read More

View Article

Download full-text PDF

Source
https://www.mdpi.com/1424-8220/19/8/1846
Publisher Site
http://dx.doi.org/10.3390/s19081846DOI Listing
April 2019
1 Read

Antioxidant effect of sildenafil: Potential hepatoprotection via differential expression of mitochondrial proteins in apolipoprotein E knockout mice.

Pharmacol Rep 2019 Jan 6;71(3):422-429. Epub 2019 Jan 6.

Laboratory of Translational Physiology, Federal University of Espírito Santo, Vitória, Brazil; Pharmaceutical Sciences Graduate Program, Vila Velha University, Vila Velha, Brazil. Electronic address:

Background: High plasma cholesterol levels are able to trigger several pathophysiological events, including inflammation, cell damage and especially oxidative stress. Previously, studies have shown that sildenafil exhibited antioxidant effects in several experimental models. Here we evaluate the role of sildenafil in liver redox equilibrium of apolipoprotein E knockout (apoE-KO) mice. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S17341140183056
Publisher Site
http://dx.doi.org/10.1016/j.pharep.2019.01.005DOI Listing
January 2019
4 Reads

Comparative study of Cu uptake and early transcriptome responses in the green microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii.

Environ Pollut 2019 Apr 10;250:331-337. Epub 2019 Apr 10.

Department F.-A. Forel for Environmental and Aquatic Sciences, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 66, Boulevard Carl-Vogt, CH-1211, Geneva 4, Switzerland. Electronic address:

Microalgae are widely used as representative primary producers in ecotoxicology, while macrophytes are much less studied. Here we compared the bioavailability and cellular toxicity pathways of 2 h-exposure to 10 mol L Cu in the macrophyte Elodea nuttallii and the green microalga Chlamydomonas reinhardtii. Uptake rate was similar but faster in the algae than in the macrophyte, while RNA-Sequencing revealed a similar number of regulated genes. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S02697491183557
Publisher Site
http://dx.doi.org/10.1016/j.envpol.2019.04.032DOI Listing
April 2019
1 Read

And three's a party: lysosomes, lipid droplets, and the ER in lipid trafficking and cell homeostasis.

Authors:
Mike Henne

Curr Opin Cell Biol 2019 Apr 16;59:40-49. Epub 2019 Apr 16.

University of Texas Southwestern Medical Center, Department of Cell Biology, Dallas, TX, 75390, USA.

Sterols and fatty acids (FAs) are essential lipids that play fundamental roles in membrane dynamics and cellular homeostasis. Synthesized at the endoplasmic reticulum (ER) and cytoplasm, trafficked by proteins, and stored in lipid droplets (LDs), much work has been conducted examining how these lipids are shuttled from one location to another. Recent work has highlighted the importance of inter-organelle crosstalk in the regulation of sterol and FA homeostasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ceb.2019.02.011DOI Listing
April 2019
1 Read

3D chitosan scaffolds impair NLRP3 inflammasome response in macrophages.

Acta Biomater 2019 Apr 16. Epub 2019 Apr 16.

i3S - Instituto de Inovação e Investigação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-125 Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Rua Alfredo Allen, 208, 4200-125 Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. Electronic address:

Chitosan (Ch) is used in different biomedical applications to promote tissue repair. However, tissue injury caused by biomaterial implantation lead to the release of danger signals that engage different inflammatory pathways on the host. Different implanted materials activate the inflammasome leading to the modulation of the immune response. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S17427061193027
Publisher Site
http://dx.doi.org/10.1016/j.actbio.2019.04.035DOI Listing
April 2019
1 Read

Oxidative stress-induced senescence markedly increases disc cell bioenergetics.

Mech Ageing Dev 2019 Apr 16. Epub 2019 Apr 16.

Department of Orthopedic Surgery, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA, 15213, USA; Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15213, USA. Electronic address:

Cellular senescence is a phenotype characterized by irreversible growth arrest, chronic elevated secretion of proinflammatory cytokines and matrix proteases, a phenomenon known as senescence associated secretory phenotype (SASP). Biomarkers of cellular senescence have been shown to increase with age and degeneration of human disc tissue. Senescent disc cells in culture recapitulate features associated with age-related disc degeneration, including increased secretion of proinflammatory cytokines, matrix proteases, and fragmentation of matrix proteins. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00476374183019
Publisher Site
http://dx.doi.org/10.1016/j.mad.2019.04.006DOI Listing
April 2019
1 Read

RTN1-C is involved in high glucose-aggravated neuronal cell subjected to oxygen-glucose deprivation and reoxygenation injury via endoplasmic reticulum stress.

Brain Res Bull 2019 Apr 16. Epub 2019 Apr 16.

Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China. Electronic address:

Background: Patients suffering from diabetes mellitus experience poor outcomes after ischemic stroke. RTN1-C, ER-associated proteins localized in endoplasmic reticulum (ER) membrane, plays an important role in ER stress-induced apoptosis and regulates cellular susceptibility to different apoptosis pathways. Overexpression of RTN1-C can aggravate cerebral ischemia/reperfusion injury (IRI). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.brainresbull.2019.04.010DOI Listing

Precise editing of plant genomes - prospects and challenges.

Semin Cell Dev Biol 2019 Apr 16. Epub 2019 Apr 16.

Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. Electronic address:

The past decade has witnessed unprecedented development in genome engineering, a process that enables targeted modification of genomes. The identification of sequence-specific nucleases such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas system, in particular, has led to precise and efficient introduction of genetic variations into genomes of various organisms. Since the CRISPR/Cas system is highly versatile, cost-effective and much superior to ZFNs and TALENs, its widespread adoption by the research community has been inevitable. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S10849521183010
Publisher Site
http://dx.doi.org/10.1016/j.semcdb.2019.04.010DOI Listing
April 2019
1 Read

Pregabalin affords retinal neuroprotection in diabetic rats: Suppression of retinal glutamate, microglia cell expression and apoptotic cell death.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Pharmacology and Toxicology, Faculty of Pharmacy (girls), Al-Azhar University, Cairo, Egypt.

Pregabalin is the first drug to receive FDA approval for treating diabetic neuropathic pain. This study investigated the neuroprotective effect of pregabalin in an experimental model of diabetic retinopathy and tested some possible mechanisms underlying the putative neuroprotective effect. Male Wistar rats received streptozotocin (45 mg/kg) to induce type 1 diabetes mellitus. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00144835183023
Publisher Site
http://dx.doi.org/10.1016/j.exer.2019.04.014DOI Listing
April 2019
1 Read

Fluid supplementation accelerates epithelial repair during chemical colitis.

PLoS One 2019 19;14(4):e0215387. Epub 2019 Apr 19.

Division of Gastroenterology, Department of Medicine, University of Miami-Leonard Miller School of Medicine, Miami, FL, United States of America.

The dextran sulfate sodium (DSS) model of colitis is a common animal model of inflammatory bowel disease that causes pain and distress. In this study, we aimed to determine whether fluid supplementation can be used as a welfare-based intervention to minimize animal suffering. C57Bl/6 females undergoing acute colitis by administration of 3% DSS in drinking water were supplemented with 1 mL intraperitoneal injections of NaCl and compared to non-supplemented control mice. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215387PLOS
April 2019
1 Read

Doxycycline, Azithromycin and Vitamin C (DAV): A potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs).

Aging (Albany NY) 2019 Apr 19. Epub 2019 Apr 19.

Translational Medicine, School of Environment and Life Sciences, Biomedical Research Centre (BRC), University of Salford, Greater Manchester, M5 4WT, United Kingdom.

Here, we devised a new strategy for eradicating cancer stem cells (CSCs), via a "synthetic-metabolic" approach, involving two FDA-approved antibiotics and a dietary vitamin supplement. This approach was designed to induce a "rho-zero-like" phenotype in cancer cells. This strategy effectively results in the synergistic eradication of CSCs, using vanishingly small quantities of two antibiotics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.101905DOI Listing

New Insight into Mycochemical Profiles and Antioxidant Potential of Edible and Medicinal Mushrooms: A Review.

Int J Med Mushrooms 2019 ;21(3):237-251

Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong, China.

Unhealthy foods, lifestyle, environmental factors, ultraviolet radiation, and other factors are responsible for overoxidation, which generates free radicals and leads to oxidative stress, cell damage, and eventually various chronic diseases. Edible and medicinal mushrooms contain large amounts of mycochemicals that possess antioxidant properties and play important roles in preventing and treating those chronic diseases. Edible and medicinal mushrooms have a strong free radical scavenging ability and are involved in various bioactivities and health-promoting benefits. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1615/IntJMedMushrooms.2019030079DOI Listing
January 2019

The interplay between microbiota-dependent metabolite trimethylamine N-oxide, Transforming growth factor β/SMAD signaling and inflammasome activation in chronic kidney disease patients: A new mechanistic perspective.

J Cell Biochem 2019 Apr 19. Epub 2019 Apr 19.

Internal Medicine Department, Faculty of Medicine, Tanta University, Tanta, Egypt.

Background: Chronic kidney disease (CKD) signifies a frequently life-threatening condition influencing kidney structure and function. Despite its irrefutable importance, its exact pathogenesis is not completely clarified. However, CKD is known to be associated with accumulated uremic toxins/metabolites, interstitial fibrosis, and systemic inflammation. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.28707
Publisher Site
http://dx.doi.org/10.1002/jcb.28707DOI Listing
April 2019
1 Read