645 results match your criteria Cell Journal[Journal]


Hardy Weinberg Equilibrium Disturbances in Case-Control Studies Lead to Non-Conclusive Results.

Authors:
Jose Luis Royo

Cell J 2021 Jan 22;22(4):572-574. Epub 2020 Apr 22.

Department of Surgery, Biochemisty and Immunology, School of Medicine, University of Malaga, Boulevar Louis Pasteur s/n, Málaga, Spain. Electronic

Recently, it has been proposed the association of a common deletion affecting toll-like receptor 2 promoter (-196 to -177) to type 2 diabetes mellitus risk. However, genotyping results show a significant deviation from the Hardy- Weinberg Equilibrium (HWE). The law of Hardy-Weinberg shows that for an autosomal biallelic marker with allele frequencies f=p and f=q, the proportion of subjects with genotypes AA, Aa, and aa should follow the following: f=p, f=2pq, and f=q. Read More

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http://dx.doi.org/10.22074/cellj.2021.7195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211273PMC
January 2021

Astaxanthin Reduces Demyelination and Oligodendrocytes Death in A Rat Model of Multiple Sclerosis.

Cell J 2021 Jan 22;22(4):565-571. Epub 2020 Apr 22.

Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic Address:

Objective: Astaxanthin (AST) is a carotenoid with anti-oxidative, anti-inflammatory, and anti-apoptotic properties. It has also been reported that AST exerts protective effects against neurodegenerative diseases and reduces oxidative stress-induced the central nervous system (CNS) injury. In this study, we aimed to evaluate the protective potential of AST in inhibiting demyelination and oligodendrocyte death in a rat model of multiple sclerosis (MS). Read More

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http://dx.doi.org/10.22074/cellj.2021.6999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211289PMC
January 2021

Differentiation Induction and Proliferation Inhibition by A Cell-Free Approach for Delivery of Exogenous miRNAs to Neuroblastoma Cells Using Mesenchymal Stem Cells.

Cell J 2021 Jan 27;22(4):556-564. Epub 2020 Apr 27.

Department of Haematology, Tarbiat Modares University, Tehran, Iran.

Objective: Neuroblastoma (NB) is one of the frequently observed malignant solid tumors of childhood and infancy, accounting for 15% of pediatric cancer deaths. Recently, the approach of differentiation therapy has shown considerable promise in effective treatment of NB patients. MiR-124 belongs to the nervous system-specific miRNAs that is increased during neuronal differentiation and may be one of the potential therapeutic targets for the treatment of NB. Read More

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http://dx.doi.org/10.22074/cellj.2021.6928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211274PMC
January 2021

Compression of The Sciatic Nerve May not Contribute to Ipsilateral Hyperalgesia Development in Ovariectomized Female Rats!

Cell J 2021 Jan 22;22(4):548-555. Epub 2020 Apr 22.

Department of Biology, Faculty of Natural Science, University of Badji Mokhtar, Annaba, Algeria.

Objective: von Frey Filament (vFF) is an aesthesiometer to measure paw withdrawal thresholds. Our aim was to validate the manually von Frey test technique for assessing neuropathic pain behavioral signs in a sciatic nerve ligation model.

Materials And Methods: In this experimental study, peripheral neuropathic pain associated with sciatic nerve chronic ligation (SN-CL) was induced. Read More

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http://dx.doi.org/10.22074/cellj.2021.6907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211275PMC
January 2021

The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature.

Cell J 2021 Jan 22;22(4):542-547. Epub 2020 Apr 22.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Objective: MicroRNAs (miRNAs) are short, noncoding RNAs that play vital roles in gene regulation. It has been shown that storage has an effect on platelet miRNAs. MiR-16 is highly expressed in platelets and it appears to target the genes involved in cell death. Read More

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http://dx.doi.org/10.22074/cellj.2021.6790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211276PMC
January 2021

The Effect of Arbutin on The Expression of Tumor Suppressor , Ratio and Oxidative Stress Induced by Tert-Butyl Hydroperoxide in Fibroblast and LNcap Cell Lines.

Cell J 2021 Jan 22;22(4):532-541. Epub 2020 Apr 22.

Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Objective: Arbutin (p-hydroxyphenyl-β-D-glucopyranoside) possesses beneficial functions including antioxidant, antiinflammatory, and anti-tumoral activities. Due to the important role of oxidative stress and apoptosis in the successful treatment of cancer, understanding mechanisms that lead to apoptosis in cancer cells, is essential. The purpose of the current study was to evaluate the effect of arbutin on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress and the related mechanisms in fibroblast and Lymph Node Carcinoma of the Prostate (LNCaP) cells. Read More

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http://dx.doi.org/10.22074/cellj.2021.6902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211292PMC
January 2021

Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from .

Cell J 2021 Jan 22;22(4):523-531. Epub 2020 Apr 22.

Key Lab for Pharmacology of Ministry of Education, Department of Pharmacology, Zunyi Medical University, Zunyi, China.

Objective: Ulcerative colitis (UC) is a long-lasting inflammatory disease of the colon. Epidemiological studies showed that the prevalence and incidence of UC are increasing worldwide in recent years. Neferine is a natural alkaloid isolated from Nelumbo nucifera Gaertn that exerts a variety of biological activities. Read More

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http://dx.doi.org/10.22074/cellj.2021.6918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211280PMC
January 2021

Diosgenin and 4-Hydroxyisoleucine from Fenugreek Are Regulators of Genes Involved in Lipid Metabolism in The Human Colorectal Cancer Cell Line SW480.

Cell J 2021 Jan 22;22(4):514-522. Epub 2020 Apr 22.

Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

Objective: Diosignin and 4-hydroxy-L-isulosine (4-OH-Ile) are the two active ingredients of Fenugreek (). Thus, in this study, we examined the effects of hydroalcoholic extract of fenugreek seeds (HEFS), diosgenin and 4-OH-Ile on the expression of acetyl-CoA carboxylase , fatty acid synthase , peroxisome proliferator-activated receptor gamma and low-density lipoprotein (LDL) receptor which are involved in lipid metabolism in SW480 cell line.

Materials And Methods: In this experimental study, SW480 cells were cultured in RPMI-1640 medium and treated with HEFS, diosignin, 4-OH-Ile or orlistat for 24 and 48 hours. Read More

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http://dx.doi.org/10.22074/cellj.2021.6751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211281PMC
January 2021
0.458 Impact Factor

A VHH-Based Anti-MUC1 Chimeric Antigen Receptor for Specific Retargeting of Human Primary T Cells to MUC1-Positive Cancer Cells.

Cell J 2021 Jan 22;22(4):502-513. Epub 2020 Apr 22.

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Objective: Immunotherapy with redirected T cells that express a chimeric antigen receptor (CAR) is a promising prospect in cancer treatment. Most CARs use murine-derived single-chain variable fragments (scFvs) as an antigen targeting moiety, which may lead to host immunogenic responses and engineered T cell disappearance. It seems that development of less immunogenic CARs, such as CARs composed of the camelid variable domain of heavy chain antibodies (VHHs) may likely overcome this obstacle. Read More

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http://dx.doi.org/10.22074/cellj.2021.6917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211288PMC
January 2021

Effects of Different Vitrification Solutions and Protocol on Follicular Ultrastructure and Revascularization of Autografted Mouse Ovarian Tissue.

Cell J 2021 Jan 22;22(4):491-501. Epub 2020 Apr 22.

Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic Adress:

Objective: Many attempts have been made to preserve fertility by improving the cryopreservation of the ovarian tissue. This current studyaimed to improve of direct cover vitrification (DCV) protocol on follicular preservation and angiogenesis in autografted ovarian tissue.

Materials And Methods: In this experimental study, sixty five female Balb/c mice (5-6 week-old) were anesthetized and their ovaries were dissected. Read More

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http://dx.doi.org/10.22074/cellj.2021.6877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211277PMC
January 2021
0.458 Impact Factor

Methylation Status of Promoter and Oligozoospermia Risk: An Epigenetic Study and in Silico Analysis.

Cell J 2021 Jan 22;22(4):482-490. Epub 2020 Apr 22.

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Electronic Address:

Objective: In this study, we evaluated the effects of promoter methylation of on oligozoospermia risk, followed by an in silico analysis.

Materials And Methods: In a case-control study, semen samples were collected from infertile and healthy control men. promoter region was amplified by methylation-specific polymerase chain reaction (PCR). Read More

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http://dx.doi.org/10.22074/cellj.2021.6498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211284PMC
January 2021

Cytokine Gene Expression Alterations in Human Macrophages Infected by .

Cell J 2021 Jan 22;22(4):476-481. Epub 2020 Apr 22.

Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic Address:

Objective: Leishmaniasis is caused by members of the species and constitute a group of infective diseases that range from cutaneous lesions to lethal visceral forms. In infected persons, macrophages recognize and eliminate the parasites via phagocytosis. In order to change a hostile environment into an environment adequate for survival and reproduction, the engulfed Leishmania species needs to modulate the function of its host macrophage. Read More

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http://dx.doi.org/10.22074/cellj.2021.6524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211285PMC
January 2021

Preimplantation Genetic Screening and The Success Rate of Fertilization: A Three-Years Study on Iranian Population.

Cell J 2021 Jan 22;22(4):467-475. Epub 2020 Apr 22.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic

Objective: fertilization (IVF) is one of the most efficient approaches within the context of assisted reproductive technology (ART) to treat infertility. High pregnancy rates have become the major index of successful IVF in clinical studies. It is not clear yet which factors are certainly responsible for IVF success, as various outcomes were obtained in different IVF centers with different settings. Read More

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http://dx.doi.org/10.22074/cellj.2021.6784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211278PMC
January 2021
0.458 Impact Factor

Influence of Follicular Fluid and Seminal Plasma on The Expression of Endometrial Receptivity Genes in Endometrial Cells.

Cell J 2021 Jan 22;22(4):457-466. Epub 2020 Apr 22.

HDepartment of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran. Electronic Address:

Objective: Endometrial receptivity plays a key role in pregnancy success in assisted reproduction cycles. Recent evidence suggests that seminal plasma (SP) and follicular fluid (FF) influence the uterine endometrium to improve implantation of the embryo and the establishment of pregnancy. In this study, we attempt to assess the influence of FF and SP on the expression levels of main endometrial receptivity genes ( and ) in endometrial stromal cells. Read More

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http://dx.doi.org/10.22074/cellj.2021.6851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211287PMC
January 2021

The Relationship between Functional Promoter Variants of Macrophage Migration Inhibitory Factor and Endometriosis.

Cell J 2021 Jan 22;22(4):450-456. Epub 2020 Apr 22.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic Address:

Objective: Endometriosis is a common gynecological and inflammatory disorder. Macrophage migration inhibitory factor (MIF) is a key pro-inflammatory cytokine that is secreted by accumulated active macrophages in ectopic endometrial tissues. Two promoter polymorphisms of [-794(CATT) /-173G/C] were identified to susceptibility and severity of several immune and inflammatory diseases. Read More

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http://dx.doi.org/10.22074/cellj.2021.6858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211282PMC
January 2021

Differential Expression Patterns of Rspondin Family and Leucine-Rich Repeat-Containing G-Protein Coupled Receptors in Chondrocytes and Osteoblasts.

Cell J 2021 Jan 22;22(4):437-449. Epub 2020 Apr 22.

Institute for Skeletal Aging and Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon, Korea.

Objective: Rspondins (RSPOs) are regarded as the significant modulators of WNT signaling pathway and they are expressed dynamically during developmental stages. Since in osteoarthritis (OA) both cartilage and subchondral bone suffer damages and WNT signaling pathway has a crucial role in their maintenance, the objective of the study was to analyze expression profile of RSPO family and its receptors [leucine-rich repeat-containing G-protein coupled receptors (LGRs)] in OA tissue samples as well as in differentiating chondrocytes and osteoblasts.

Materials And Methods: In this experimental study, human early and advanced stage of OA tissue samples were analyzed for the morphological changes of articular cartilage by hematoxylin and eosin (H and E) staining, safranin-O staining and lubricin immunostaining. Read More

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http://dx.doi.org/10.22074/cellj.2021.6927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211279PMC
January 2021

Correlation of and Expressions with Proliferation Potential of Early and Late Culture of Human Peripheral Blood Mesenchymal Stem Cells.

Cell J 2021 Jan 22;22(4):431-436. Epub 2020 Apr 22.

Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Objective: In the recent years, mesenchymal stem cells (MSCs) were considered as the suitable source of cells for transplantation into the damaged tissues in regenerative medicine. There was low number of these cells in different organs and this characteristic was the main drawback to use them in treatment of diseases. Cellular senescence of the stem cells has been demonstrated to be dependent to the telomerase activity. Read More

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http://dx.doi.org/10.22074/cellj.2021.6920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211286PMC
January 2021

Changes in Serum Levels and Gene Expression of in The Cardiac Muscle of Diabetic Rats: The Effect of Dichloroacetate and Endurance Training.

Cell J 2021 Jan 22;22(4):425-430. Epub 2020 Apr 22.

Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Objective: Physical activity leads to changes in the level of gene expression in different kinds of cells, including changes in mitochondrial biogenesis in the myocardium in diabetic patients. Peroxisome proliferator-activated receptor coactivator 1α (PGC-1α) is a gene that plays an important role in regulating mitochondrial biogenesis. The purpose of this study was to investigate changes in serum levels and cardiac muscle expression of in diabetic rats in response to the administration of dichloroacetate (DCA) and endurance training. Read More

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http://dx.doi.org/10.22074/cellj.2021.6942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211283PMC
January 2021

A Moderate Increase in Ambient Temperature Influences The Structure and Hormonal Secretion of Adrenal Glands in Rats.

Cell J 2021 Jan 22;22(4):415-424. Epub 2020 Apr 22.

Department of Cytology, Institute for Biological Research Siniša Stanković, University of Belgrade, 142 Despot Stefan Blvd., Belgrade, Republic of Serbia.

Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats. Read More

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http://dx.doi.org/10.22074/cellj.2021.6827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211272PMC
January 2021

Enhancement of The Stability of Human Growth Hormone by Using Tris(hydroxymethyl)aminomethane: Molecular Docking and Experimental Analysis.

Cell J 2021 Jan 22;22(4):406-414. Epub 2020 Apr 22.

Department of Pharmaceutical Sciences, Nova Southeastern University, Pharmaceutical Sciences, Davie, Florida, USA.

Objective: It is so difficult to formulate human growth hormone (hGH) in a solution with high stability and new drug delivery system (NDDs) due to physiochemical instability. The purpose of this study was to investigate the possibility of using Tris as a hGH stabilizer.

Materials And Methods: In this experimental study, the role of tris(hydroxymethyl)aminomethane (Tris) was evaluated as a hGH stabilizing agent in phosphate buffer, as a practical aqueous solution and a media to release NDDs. Read More

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http://dx.doi.org/10.22074/cellj.2021.6903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211290PMC
January 2021

The Immunomodulatory Effect of Radiofrequency Electromagnetic Field on Serum Cytokine Levels in A Mouse Model of Hindlimb Unloading.

Cell J 2021 Jan 22;22(4):401-405. Epub 2020 Apr 22.

Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic Address:

Objective: Astronauts are exposed to a wide range of environmental stresses during spaceflights that reduce their immune responses and make them more susceptible to infections and malignancies. Exposure to a low dose of a certain stress induces an adaptive response, which leads to resistance to higher doses of the same or other types of stress. We designed this study to investigate the effect of radiofrequency electromagnetic field (RF-EMF)-induced adaptive response on immune system modulation in a mouse model of hindlimb unloading (HU) as a ground-based animal model of spaceflight conditions. Read More

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http://dx.doi.org/10.22074/cellj.2021.6856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211291PMC
January 2021

Endoplasmic Reticulum Stress Induces miR-706, A Pro-Cell Death microRNA, in A Protein Kinase RNA-Like ER Kinase (PERK) and Activating Transcription Factor 4 (ATF4) Dependent Manner.

Cell J 2020 Oct 15;22(3):394-400. Epub 2019 Dec 15.

The Second Department of Urology, Shenyang Red Cross Hospital, Shenyang, People's Republic of China (CHN).

Objective: Endoplasmic reticulum (ER) stress causes an adaptive response initiated by protein kinase RNA-like ER kinase (PERK), Ire1 and ATF6. It has been reported that these upstream regulators induce microRNAs. The current study was designed to find a novel microRNA that mediates ER stress components and finally contributes to cell fate decision. Read More

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http://dx.doi.org/10.22074/cellj.2020.6873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947010PMC
October 2020

Gene Expression Microarray Data Meta-Analysis Identifies Candidate Genes and Molecular Mechanism Associated with Clear Cell Renal Cell Carcinoma.

Cell J 2020 Oct 15;22(3):386-393. Epub 2019 Dec 15.

Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, China. Electronic

Objective: We aimed to explore potential molecular mechanisms of clear cell renal cell carcinoma (ccRCC) and provide candidate target genes for ccRCC gene therapy.

Materials And Methods: This is a bioinformatics-based study. Microarray datasets of GSE6344, GSE781 and GSE53000 were downloaded from Gene Expression Omnibus database. Read More

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http://dx.doi.org/10.22074/cellj.2020.6561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947001PMC
October 2020

lncRNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 Promotes Proliferation and Invasion of Non-Small Cell Lung Cancer Cells via Down-Regulating miR-202 Expression.

Cell J 2020 Oct 15;22(3):375-385. Epub 2019 Dec 15.

Department of Oncology, Guangzhou Panyu Hospital of Chinese Medicine, Guangzhou, PR China.

Objective: Accumulating evidences indicate that long non-coding RNAs (lncRNAs) play key roles in cancer. This study aims to clarify role of the metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in non-small cell lung cancer (NSCLC) and uncover the underlying mechanisms.

Materials And Methods: In this experimental study, and expression in tissues and cell lines were detected using quantitative real time polymerase chain reaction (qRT-PCR) assay. Read More

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http://dx.doi.org/10.22074/cellj.2020.6837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947012PMC
October 2020

Quality of Blastocysts Created by Embryo Splitting: A Time-Lapse Monitoring and Chromosomal Aneuploidy Study.

Cell J 2020 Oct 15;22(3):367-374. Epub 2019 Dec 15.

Abortion Research Center, Yazd Institute of Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Objective: The aim of this study was to screen the potential of human embryos to develop into expanding blastocysts following embryo splitting and then assess the quality of the generated blastocysts based on chromosomal characteristics and using morphokinetics.

Materials And Methods: In this experimental study, a total of 82 good quality cleavage-stage donated embryos (8- 14 cells) were used (24 embryos were cultured to the blastocyst stage as controls and 58 embryos underwent splitting). After splitting, the blastomere donor and blastomere recipient embryos were named twin A and twin B, respectively. Read More

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http://dx.doi.org/10.22074/cellj.2020.6717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946999PMC
October 2020

Supplementation of Culture Media with Lysophosphatidic Acid Improves The Follicular Development of Human Ovarian Tissue after Xenotransplantaion into The Back Muscle of γ-Irradiated Mice.

Cell J 2020 Oct 15;22(3):358-366. Epub 2019 Dec 15.

Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic Address:

Objective: The aim of the present study was to evaluate the effects of lysophosphatidic acid (LPA) supplementation of human ovarian tissue culture media on tissue survival, follicular development and expression of apoptotic genes following xenotransplantation.

Materials And Methods: In this experimental study, human ovarian tissue was collected from eight normal female to male transsexual individuals and cut into small fragments. These fragments were vitrified-warmed and cultured for 24 hours in the presence or absence of LPA, then xenografted into back muscles of γ-irradiated mice. Read More

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http://dx.doi.org/10.22074/cellj.2020.6752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947004PMC
October 2020

Folliculogenesis-Associated Genes Expression in Human Vitrified Ovarian Tissue after Xenotransplantation in γ-Irradiated Mice.

Cell J 2020 Oct 15;22(3):350-357. Epub 2019 Dec 15.

Department of Anatomical Sciences, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran. Electronic Address:

Objective: Autograft transplantation of vitrified cortical ovarian tissue is an acceptable clinical technique for fertility preservation in women. Xenograft transplantation into animal models could be useful for evaluating the safety of human vitrified ovarian tissue. This study targeted to evaluate impact of vitrification on expression of the genes associated with folliculogenesis after xenograft transplantation of human vitrified ovarian tissue to γ-irradiated mice. Read More

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http://dx.doi.org/10.22074/cellj.2020.6553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947005PMC
October 2020
0.458 Impact Factor

Extraction and Evaluation of Outer Membrane Vesicles from Two Important Gut Microbiota Members, Bacteroides fragilis and Bacteroides thetaiotaomicron.

Cell J 2020 Oct 15;22(3):344-349. Epub 2019 Dec 15.

Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran.

Objective: The gastrointestinal tract (GI) is colonized by a complex microbial community of gut microbiota. Bacteroides spp. have significant roles in gut microbiota and they host interactions by various mechanisms, including outer membrane vesicle (OMVs) production. Read More

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http://dx.doi.org/10.22074/cellj.2020.6499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947009PMC
October 2020

Allograft of Sertoli Cell Transplantation in Combination with Memantine Alleviates Ischemia-Induced Tissue Damages in An Animal Model of Rat.

Cell J 2020 Oct 15;22(3):334-343. Epub 2019 Dec 15.

Department of Anatomy and Cell Biology, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.

Objective: Brain ischemia is the most common disease in the world caused by the disruption of the blood supply of brain tissue. Cell therapy is one of the new and effective strategies used for the prevention of brain damages. Sertoli cells (SCs) can hide from the host immune system and secrete trophic factors. Read More

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http://dx.doi.org/10.22074/cellj.2020.6689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947000PMC
October 2020

PolyI:C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling.

Cell J 2020 Oct 15;22(3):325-333. Epub 2019 Dec 15.

Department of Paediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China. Electronic Address

Objective: This study aimed to evaluate the specific roles of polyinosinic:polycytidylic acid (polyI:C) in macrophage chemotaxis and reveal the potential regulatory mechanisms related to chemokine receptor 5 ().

Materials And Methods: In this experimental study, THP-1-derived macrophages (THP1-Mφs) induced from THP- 1 monocytes were treated with 25 μg/mL polyI:C. Toll-like receptor 3 (), Jumonji domain-containing protein (JMJD)1A, and small interfering RNA (siRNAs) were transfected into THP1-Mφs. Read More

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http://dx.doi.org/10.22074/cellj.2020.6713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947015PMC
October 2020

Adverse Drug Reaction Detection in Social Media by Deepm Learning Methods.

Cell J 2020 Oct 15;22(3):319-324. Epub 2019 Dec 15.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Objective: Health-related studies have been recently at the heart attention of the media. Social media, such as Twitter, has become a valuable online tool to describe the early detection of various adverse drug reactions (ADRs). Different medications have adverse effects on various cells and tissues, sometimes more than one cell population would be adversely affected. Read More

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http://dx.doi.org/10.22074/cellj.2020.6615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947008PMC
October 2020

Cytotoxicity Evaluation of The Bioresorbable and Titanium Plates/Screws Used in Maxillofacial Surgery on Gingival Fibroblasts and Human Mesenchymal Bone Marrow Stem Cells.

Cell J 2020 Oct 15;22(3):310-318. Epub 2019 Dec 15.

Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic Address:

Objective: Bioresorbable and titanium plates/screws are considered as a standard treatment for fixation of the bone segments of craniofacial area and paying attention to their biocompatibility is an important issue along with other aspects of application. The purpose of the study was to evaluate the cell viability of two types of plate and screw used in maxillofacial surgeries in contact with gingival fibroblasts and bone marrow stem cells.

Materials And Methods: In this experimental study after extraction and cultivation of cells from healthy human gingival tissue and alveolar bone of jaw, cytotoxicity of device was evaluated. Read More

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http://dx.doi.org/10.22074/cellj.2020.6409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947002PMC
October 2020

Protective Effect of Royal Jelly against Cyclophosphamide-Induced Thrombocytopenia and Spleen and Bone Marrow Damages in Rats.

Cell J 2020 Oct 15;22(3):302-309. Epub 2019 Dec 15.

Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic Address:

Objective: Despite the effective role of chemotherapy in cancer treatment, several side effects have been reported to date. For instance, Cyclophosphamide (CP) induces deleterious effects on both cancer and normal cells. Royal jelly (RJ) has a lot of beneficial properties, such as anti-oxidant and anti-inflammatory activities. Read More

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http://dx.doi.org/10.22074/cellj.2020.6703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947013PMC
October 2020

Short Pretreatment with Calcitriol Is Far Superior to Continuous Treatment in Stimulating Proliferation and Osteogenic Differentiation of Human Adipose Stem Cells.

Cell J 2020 Oct 15;22(3):293-301. Epub 2019 Dec 15.

Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands.

Objective: This study investigated whether short stimulation (30 minutes) of human adipose stem cells (hASCs) with 1,25-dihydroxyvitamin D (calcitriol or 1,25-(OH)VitD), fitting within the surgical procedure time frame, suffices to induce osteogenic differentiation, and compared this with continuous treatment with 1,25-(OH)VitD.

Materials And Methods: In this experimental study, hASCs were pretreated with/without 10 nM calcitriol for 30 minutes, seeded on biphasic calcium phosphate (BCP), and cultured for 3 weeks with/without 1,25-(OH)VitD. Cell attachment was determined 30 minutes after cell seeding. Read More

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http://dx.doi.org/10.22074/cellj.2020.6773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947014PMC
October 2020

Kinetics of Oncolytic Reovirus T3D Replication and Growth Pattern in Mesenchymal Stem Cells.

Cell J 2020 Oct 15;22(3):283-292. Epub 2019 Dec 15.

Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA.

Objective: Currently, application of oncolytic-virus in cancer treatment of clinical trials are growing. Oncolytic-reovirus is an attractive anti-cancer therapeutic agent for clinical testing. Many studies used mesenchymal stem cells (MSCs) as a carrier cell to enhance the delivery and quality of treatment with oncolytic-virotherapy. Read More

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http://dx.doi.org/10.22074/cellj.2020.6686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947011PMC
October 2020

Cardiac Differentiation of Adipose Tissue-Derived Stem Cells Is Driven by BMP4 and bFGF but Counteracted by 5-Azacytidine and Valproic Acid.

Cell J 2020 Oct 15;22(3):273-282. Epub 2019 Dec 15.

Department of Stem Cells and Regenerative Medicine, Institute for Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. Elrctronic

Objective: Bone morphogenetic protein 4 (BMP4) and basic fibroblast growth factor (bFGF) play important roles in embryonic heart development. Also, two epigenetic modifying molecules, 5'-azacytidine (5'-Aza) and valproic acid (VPA) induce cardiomyogenesis in the infarcted heart. In this study, we first evaluated the role of BMP4 and bFGF in cardiac trans-differentiation and then the effectiveness of 5´-Aza and VPA in reprogramming and cardiac differentiation of human adipose tissue-derived stem cells (ADSCs). Read More

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http://dx.doi.org/10.22074/cellj.2020.6582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947007PMC
October 2020

The Combination of Metformin and Disulfiram-Cu for Effective Radiosensitization on Glioblastoma Cells.

Cell J 2020 Oct 15;22(3):263-272. Epub 2019 Dec 15.

Department of Pharmacology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Objective: Glioblastoma (GBM) is one of the devastating types of primary brain tumors with a negligible response to standard therapy. Repurposing drugs, such as disulfiram (DSF) and metformin (Met) have shown antitumor properties in different cell lines, including GBM. In the present study, we focused on the combinatory effect of Met and DSF-Cu on the induction of apoptosis in U87-MG cells exposed to 6-MV X-ray beams. Read More

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http://dx.doi.org/10.22074/cellj.2020.6798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947006PMC
October 2020

Effects of Sorafenib and Arsenic Trioxide on U937 and KG-1 Cell Lines: Apoptosis or Autophagy?

Cell J 2020 Oct 15;22(3):253-262. Epub 2019 Dec 15.

Molecular Medicine Research Center (MMRC), Hormozgan University of Medical Science (HUMS), Bandar Abbass, Iran.

Objective: Acute myeloid leukemia (AML) is a clonal disorder of hemopoietic progenitor cells. The Raf serine/threonine (Ser/Thr) protein kinase isoforms including B-Raf and RAF1, are the upstream in the MAPK cascade that play essential functions in regulating cellular proliferation and survival. Activated autophagy-related genes have a dual role in both cell death and cell survival in cancer cells. Read More

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http://dx.doi.org/10.22074/cellj.2020.6728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947003PMC
October 2020
0.458 Impact Factor

The Effects of Embryonic Cerebrospinal Fluid on The Viability and Neuronal Differentiation of Adipose Tissue-Derived Stem Cells in Wistar Rats.

Cell J 2020 Jul 14;22(2):245-252. Epub 2019 Oct 14.

Faculty of Biology, Medicine and Health Division of Neuroscience and Experimental Psychology, The University of Manchester, Manchester, UK.

Objective: The embryonic cerebrospinal fluid (e-CSF) contains various growth factors and morphogens. Recent studies showed that e-CSF plays significant roles in embryonic brain development. Adipose tissue-derived stem cells (ADSCs) have a mesodermal origin that can be differentiated into mesodermal and ectodermal lineages. Read More

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http://dx.doi.org/10.22074/cellj.2020.6560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874795PMC
July 2020
0.458 Impact Factor

Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells.

Cell J 2020 Jul 14;22(2):236-244. Epub 2019 Oct 14.

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic

Objective: Mesenchymal stem cells (MSCs) have prominent immunomodulatory roles in the tumor microenvironment. The current study intended to elucidate Treg subsets and their cytokines after exposing naïve T lymphocytes to adiposederived MSCs (ASCs).

Materials And Methods: In this experimental study, to obtain ASCs, breast adipose tissues of a breast cancer patient and a normal individual were used. Read More

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http://dx.doi.org/10.22074/cellj.2020.6313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874788PMC

Optimizing The Cell Seeding Protocol to Human Decellularized Ovarian Scaffold: Application of Dynamic System for Bio-Engineering.

Cell J 2020 Jul 14;22(2):227-235. Epub 2019 Oct 14.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Electronic address:

Objective: Decellularized tissue scaffolds provide an extracellular matrix to control stem cells differentiation toward specific lineages. The application of mesenchymal stem cells for artificial ovary production may enhance functions of the ovary. On the other hand, the scaffold needs interaction and integration with cells. Read More

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http://dx.doi.org/10.22074/cellj.2020.6604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874796PMC

Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study.

Cell J 2020 Jul 14;22(2):218-226. Epub 2019 Oct 14.

Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Objective: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process.

Materials And Methods: In this numerical study, we based the separation process on the differences in the particle sizes. Read More

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http://dx.doi.org/10.22074/cellj.2020.6386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874797PMC

Platelets Apoptosis and Clearance in The Presence of Sodium Octanoate during Storage of Platelet Concentrate at 4˚C.

Cell J 2020 Jul 14;22(2):212-217. Epub 2019 Oct 14.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Objective: Platelet (PLT) storage at 4˚C has several benefits, however, it is accompanied by increased clearance of PLTs after transfusion. In this study, we evaluated the potential of sodium octanoate (SO) for reducing apoptosis and clearance rate of PLTs after long-term storage in cold.

Materials And Methods: In this experimental study, PLT concentrates (PCs) were stored for 5 days under the following three conditions: 20-24˚C, 4˚C, and 4˚C in the presence of SO. Read More

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http://dx.doi.org/10.22074/cellj.2020.6697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874783PMC

In Utero Exposure to Gestational Diabetes Alters DNA Methylation and Gene Expression of CDKN2A/B in Langerhans Islets of Rat Offspring.

Cell J 2020 Jul 14;22(2):203-211. Epub 2019 Oct 14.

Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran. Electronic Address:

Objective: DNA methylation, a major epigenetic reprogramming mechanism, contributes to the increased prevalence of type 2 diabetes mellitus (T2DM). Based on genome-wide association studies, polymorphisms in are associated with T2DM. Our previous studies showed that gestational diabetes mellitus (GDM) causes apoptosis in β-cells, leading to a reduction in their number in pancreatic tissue of GDM-exposed adult rat offspring. Read More

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http://dx.doi.org/10.22074/cellj.2020.6699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874789PMC

Arsenic Trioxide and Thalidomide Combination Induces Autophagy Along with Apoptosis in Acute Myeloid Cell Lines.

Cell J 2020 Jul 14;22(2):193-202. Epub 2019 Oct 14.

Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic

Objective: Autophagy and apoptosis play key roles in cancer survival and pathogenesis and are governed by specific genes which have a dual role in both cell death and survival. Arsenic trioxide (ATO) and thalidomide (THAL) are used for treatment of many types of hematologic malignancies. ATO prevents the proliferation of cells and induces apoptosis in some cancer cells. Read More

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http://dx.doi.org/10.22074/cellj.2020.6469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874786PMC
July 2020
0.458 Impact Factor

The Dilemma of TP53 Codon 72 Polymorphism (rs1042522) and Breast Cancer Risk: A Case-Control Study and Meta-Analysis in The Iranian Population.

Cell J 2020 Jul 14;22(2):185-192. Epub 2019 Oct 14.

Department of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran. Electronivc Address:

Objective: Mutations of as a tumor suppressor gene are frequently observed in different types of cancer. A codon 72 polymorphism located on exon 4 with two alleles encoding either Proline (CCC) or Arginine (CGC) has been indicated as a common variation in association with cancers. Controversial results have been reported regarding the association of allelic polymorphism of codon 72 of gene and breast cancer risk in Iranian patients. Read More

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http://dx.doi.org/10.22074/cellj.2020.6458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874791PMC

A Comparative Study of HOTAIR Expression in Breast Cancer Patient Tissues and Cell Lines.

Cell J 2020 Jul 14;22(2):178-184. Epub 2019 Oct 14.

Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina. Electronic

Objective: Recent data suggest that increased levels of the long non-coding RNA (lncRNA) are involved in the development of various types of malignancy, including breast cancer. The aim of present study was to investigate lncRNA expression profile in breast cancer (BC) patients and cell lines.

Materials And Methods: In this experimental study, expression level of lncRNA was evaluated in BC and normal tissues of 15 patients as well as MDA-MB-231, MCF-7 and MCF-10A cell lines, using quantitative reversetranscription polymerase chain reaction (qRT-PCR). Read More

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http://dx.doi.org/10.22074/cellj.2020.6543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874785PMC

Investigation of VASA Gene and Protein Expression in Neonate and Adult Testicular Germ Cells in Mice In Vivo and In Vitro.

Cell J 2020 Jul 14;22(2):171-177. Epub 2019 Oct 14.

Institute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld, Heidelberg, Germany.

Objective: We aimed to examine the expression levels of the VASA gene and protein in testis sections of neonate and adult mice as well as testicular cell cultures.

Materials And Methods: In this experimental study, in order to investigate the expression of this germ cell marker gene in more detail, we analyzed the expression of VASA by immunocytochemistry, immunohistochemistry and fluidigm reverse transcription-polymerase chain reaction (RT-PCR).

Results: The immunohistochemical assays showed that the VASA protein was exclusively expressed in germ cells in the seminiferous tubules of the neonate and adult testis and not in somatic cells. Read More

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http://dx.doi.org/10.22074/cellj.2020.6619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874794PMC

Evaluation of The Expression Levels of Three Long Non-Coding RNAs in Multiple Sclerosis.

Cell J 2020 Jul 14;22(2):165-170. Epub 2019 Oct 14.

Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran.

Objective: Multiple sclerosis (MS) is a chronic disorder involving both inflammatory and neurodegenerative responses. Long non-coding RNAs (lncRNAs) have been had an emerging role as the biomarkers of different disorders, including autoimmune diseases. Previous studies have shown that NR_003531. Read More

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http://dx.doi.org/10.22074/cellj.2020.6555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874792PMC
July 2020
0.458 Impact Factor

Hsa-miR-587 Regulates TGFβ/SMAD Signaling and Promotes Cell Cycle Progression.

Cell J 2020 Jul 14;22(2):158-164. Epub 2019 Oct 14.

Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic

Objective: Transforming growth factor beta/single mothers against decapentaplegic (TGFβ/SMAD) signaling pathway plays important roles in various biological processes. It acts as a tumor suppressor during the early stages of cancer progression. Discovering the regulators of this pathway provides important options for therapeutic strategies. Read More

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http://dx.doi.org/10.22074/cellj.2020.6483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874787PMC