251 results match your criteria Cell Division[Journal]


Caffeine as a tool for investigating the integration of Cdc25 phosphorylation, activity and ubiquitin-dependent degradation in .

Cell Div 2020 29;15:10. Epub 2020 Jun 29.

Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, Gothenburg, SE- 405 30 Sweden.

The evolutionarily conserved Cdc25 phosphatase is an essential protein that removes inhibitory phosphorylation moieties on the mitotic regulator Cdc2. Together with the Wee1 kinase, a negative regulator of Cdc2 activity, Cdc25 is thus a central regulator of cell cycle progression in . The expression and activity of Cdc25 is dependent on the activity of the Target of Rapamycin Complex 1 (TORC1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-00066-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322915PMC

The G2-to-M transition from a phosphatase perspective: a new vision of the meiotic division.

Cell Div 2020 25;15. Epub 2020 May 25.

Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS, Sorbonne Université, CNRS, 75005 Paris, France.

Cell division is orchestrated by the phosphorylation and dephosphorylation of thousands of proteins. These post-translational modifications underlie the molecular cascades converging to the activation of the universal mitotic kinase, Cdk1, and entry into cell division. They also govern the structural events that sustain the mechanics of cell division. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-00065-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249327PMC

EZH2-inhibitor DZNep enhances apoptosis of renal tubular epithelial cells in presence and absence of cisplatin.

Cell Div 2020 25;15. Epub 2020 May 25.

Special Medical Service Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282 China.

Background: The enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase and induces the trimethylation of histone H3 lysine 27 (H3K27me3) in the promoter of many key genes; EZH2 acts as a transcriptional repressor and is an epigenetic regulator for several cancers. However, the role of EZH2 in nonneoplastic diseases, such as kidney diseases, is unknown and has been investigated.

Materials And Method: NRK-52E cells were treated with DZNep, a potent inhibitor of EZH2, with different concentrations and for different times to evaluate the apoptosis level of NRK-52E cells by Western blot and Flow cytometry analysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-00064-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249628PMC

Toll signaling promotes JNK-dependent apoptosis in .

Cell Div 2020 10;15. Epub 2020 Mar 10.

1Institute of Intervention Vessel, Shanghai 10th People's Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092 China.

Background: Apoptosis plays pivotal roles in organ development and tissue homeostasis, with its major function to remove unhealthy cells that may compromise the fitness of the organism. Toll signaling, with the ancient evolutionary origin, regulates embryonic dorsal-ventral patterning, axon targeting and degeneration, and innate immunity. Using as a genetic model, we characterized the role of Toll signaling in apoptotic cell death. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-00062-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063707PMC

Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes.

Cell Div 2020 6;15. Epub 2020 Mar 6.

1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122 Fujian China.

Background: Microtubule organization is essential for bipolar spindle assembly and chromosome segregation, which contribute to genome stability. Kinesin-5 Eg5 is known to be a crucial regulator in centrosome separation and spindle assembly in mammalian somatic cells, however, the functions and mechanisms of Eg5 in male meiotic cell division remain largely unknown.

Results: In this study, we have found that Eg5 proteins are expressed in mouse spermatogonia, spermatocytes and spermatids. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-00063-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060529PMC

IGFBP5 increases cell invasion and inhibits cell proliferation by EMT and Akt signaling pathway in Glioblastoma multiforme cells.

Cell Div 2020 27;15. Epub 2020 Feb 27.

1Brain Tumor Research Center, Beijing Neurosurgical Institute, Capital Medical University, Beijing, 100070 People's Republic of China.

Background: Recurrence of Glioblastoma multiforme (GBM) seems to be the rule despite combination therapies. Cell invasion and cell proliferation are major reasons for recurrence of GBM. And insulin-like growth factor binding protein 5 (IGFBP5) is the most conserved of the IGFBPs and is frequently dysregulated in cancers and metastatic tissues. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-00061-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047354PMC
February 2020

A positive feedback loop between EZH2 and NOX4 regulates nucleus pulposus cell senescence in age-related intervertebral disc degeneration.

Cell Div 2020 1;15. Epub 2020 Feb 1.

Department of Orthopedics, Xinqiao Hospital, Army Medical University, Xinqiao Main Street 183, Shapingba District, Chongqing, People's Republic of China.

Background: The senescence of nucleus pulposus (NP) cells plays a vital role in the pathogenesis of intervertebral disc (IVD) degeneration (IDD). NADPH oxidase 4 (NOX4)-associated oxidative stress has been shown to induce premature NP cell senescence. Enhancer of zeste homolog 2 (EZH2) is a crucial gene regulating cell senescence. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-0060-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995653PMC
February 2020
3.526 Impact Factor

Coupled cycling and regulation of metazoan morphogenesis.

Cell Div 2020 27;15. Epub 2020 Jan 27.

IDR/Westmead Institute for Medical Research, Westmead, Australia.

Metazoan animals are characterized by restricted phenotypic heterogeneity (i.e. morphological disparity) of organisms within various species, a feature that contrasts sharply with intra-species morphological diversity observed in the plant kingdom. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-020-0059-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986050PMC
January 2020

The essentiality landscape of cell cycle related genes in human pluripotent and cancer cells.

Cell Div 2019 23;14:15. Epub 2019 Dec 23.

2Department of Genetics, Institute of Life Sciences, The Hebrew University, Givat-Ram, 9190401 Jerusalem, Israel.

Background: Cell cycle regulation is a complex system consisting of growth-promoting and growth-restricting mechanisms, whose coordinated activity is vital for proper division and propagation. Alterations in this regulation may lead to uncontrolled proliferation and genomic instability, triggering carcinogenesis. Here, we conducted a comprehensive bioinformatic analysis of cell cycle-related genes using data from CRISPR/Cas9 loss-of-function screens performed in four cancer cell lines and in human embryonic stem cells (hESCs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0058-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927170PMC
December 2019

Mechanisms for the temporal regulation of substrate ubiquitination by the anaphase-promoting complex/cyclosome.

Cell Div 2019 23;14:14. Epub 2019 Dec 23.

School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067 India.

The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit, multifunctional ubiquitin ligase that controls the temporal degradation of numerous cell cycle regulatory proteins to direct the unidirectional cell cycle phases. Several different mechanisms contribute to ensure the correct order of substrate modification by the APC/C complex. Recent advances in biochemical, biophysical and structural studies of APC/C have provided a deep mechanistic insight into the working of this complex ubiquitin ligase. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0057-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927175PMC
December 2019

FGF-induced regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway.

Cell Div 2019 25;14:13. Epub 2019 Nov 25.

Orthopaedics, Hunan Key Laboratory of Tumor Models and Individualized Medicine, The Second Xiangya Hospital, No. 139 Renming Road, Changsha, 410010 Hunan People's Republic of China.

Background: Fibroblast growth factor (FGF) and tumor growth factor-β (TGFβ) have emerged as pivotal regulators during the progression of osteosarcoma (OS). LHX9 is one crucial transcription factor controlled by FGF, however, its function in OS has not been investigated yet.

Methods: The expression of , , , -, , - and metastasis-related proteins was measured by real-time quantitative PCR (RT-qPCR) and Western blot. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0056-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876112PMC
November 2019

CRY arrests Cop1 to regulate circadian rhythms in mammals.

Authors:
Choogon Lee

Cell Div 2019 2;14:12. Epub 2019 Nov 2.

Department of Biomedical Sciences, Program in Neuroscience, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306 USA.

Cryptochromes (CRYs) are UVA and blue light photoreceptors present in all major evolutionary lineages ranging from cyanobacteria to plants and animals, including mammals. In plants, blue light activates CRYs to induce photomorphogenesis by inhibiting the CRL4 E3 ligase complex which regulates the degradation of critical transcription factors involved in plant development and growth. However, in mammals, CRYs do not physically interact with Cop1, and of course mammals are not photomorphogenic, leading to the belief that the CRY-Cop1 axis is not conserved in mammals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0055-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825355PMC
November 2019

MicroRNAs' control of cancer cell dormancy.

Authors:
Tatiana G Ruksha

Cell Div 2019 10;14:11. Epub 2019 Oct 10.

Department of Pathological Physiology, Krasnoyarsk State Medical University, P. Zeleznyaka str., 1, Krasnoyarsk, 660022 Russia.

'Dormancy', in the context of carcinogenesis, is a biological phenomenon of decreased cancer cell proliferation and metabolism. In view of their ability to remain quiescent, cancer cells are able to avoid cell death induced by chemotherapeutic agents, and thereby give rise to tumor relapse at a later stage. Being a dynamic event, the dormant state is controlled by several epigenetic mechanisms, including the action of microRNAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0054-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785928PMC
October 2019
2 Reads

Correction to: Reactivation of TAp73 tumor suppressor by protoporphyrin IX, a metabolite of aminolevulinic acid, induces apoptosis in 53-deficient cancer cells.

Cell Div 2019;14:10. Epub 2019 Aug 29.

2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Biomedicum, Solnavägen 9, 171 65 Stockholm, Sweden.

[This corrects the article DOI: 10.1186/s13008-018-0043-3.]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0052-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714447PMC

Phosphatidylinositol-5-phosphate 4-kinase gamma accumulates at the spindle pole and prevents microtubule depolymerization.

Cell Div 2019 21;14. Epub 2019 Aug 21.

Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 115 Taiwan.

Background: A previous screen of a human kinase and phosphatase shRNA library to select genes that mediate arsenite induction of spindle abnormalities resulted in the identification of phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PIP4KIIγ), a phosphatidylinositol 4,5-bisphosphate (PIP2)-synthesizing enzyme. In this study, we explored how PIP4KIIγ regulates the assembly of mitotic spindles.

Results: PIP4KIIγ accumulates at the spindle pole before anaphase, and is required for the assembly of functional bipolar spindles. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0053-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702725PMC

Septin and Ras regulate cytokinetic abscission in detached cells.

Cell Div 2019 21;14. Epub 2019 Aug 21.

1Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Box 582, 751 23 Uppsala, Sweden.

Background: Integrin-mediated adhesion is normally required for cytokinetic abscission, and failure in the process can generate potentially oncogenic tetraploid cells. Here, detachment-induced formation of oncogenic tetraploid cells was analyzed in non-transformed human BJ fibroblasts and BJ expressing SV40LT (BJ-LT) ± overactive HRas.

Results: In contrast to BJ and BJ-LT cells, non-adherent BJ-LT-Ras cells recruited ALIX and CHMP4B to the midbody and divided. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0051-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702736PMC

Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis.

Cell Div 2019 3;14. Epub 2019 Aug 3.

1School of Natural System, Institute of Natural Science and Technology, Kanazawa University, Kanazawa, Japan.

Background: Nuclear pore complexes (NPCs) act as nano-turnstiles within nuclear membranes between the cytoplasm and nucleus of mammalian cells. NPC proteins, called nucleoporins (Nups), mediate trafficking of proteins and RNA into and out of the nucleus, and are involved in a variety of mitotic processes. We previously reported that Nup62 localizes to the centrosome and mitotic spindle during mitosis, and plays a role in centrosome homeostasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0050-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679547PMC
August 2019
1 Read

Overexpression of P16 reversed the MDR1-mediated DDP resistance in the cervical adenocarcinoma by activating the ERK1/2 signaling pathway.

Cell Div 2019 6;14. Epub 2019 Jul 6.

1Medical College of Nanchang University, No.461, Bayi Street, Nanchang, 330006 Jiangxi China.

Background: To investigate the role of P16 (INK4a)-extracellular signal related kinase 1/2 (ERK1/2) signaling pathway in cisplatin (DDP) resistance induced by multidrug resistance protein 1 (MDR1), also known as P-glycoprotein (P-gp), in cervical adenocarcinoma.

Methods: A human DDP-resistant HeLa cell line (HeLa/DDP) was constructed using the combination of incremental and intermittent administration of DDP. Cell Counting Kit-8 (CCK-8) assay was used to measure the IC50 and resistance index (RI) of cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0048-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612198PMC
July 2019
3 Reads

M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s.

Cell Div 2019 15;14. Epub 2019 Jun 15.

1Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH UK.

Background: Drugs such as taxanes, epothilones, and vinca alkaloids are widely used in the treatment of breast, ovarian, and lung cancers but come with major side effects such as neuropathy and loss of neutrophils and as single agents have a lack of efficacy. M2I-1 (MAD2 inhibitor-1) has been shown to disrupt the CDC20-MAD2 interaction, and consequently, the assembly of the mitotic checkpoint complex (MCC).

Results: We report here that M2I-1 can significantly increase the sensitivity of several cancer cell lines to anti-mitotic drugs, with cell death occurring after a prolonged mitotic arrest. Read More

View Article

Download full-text PDF

Source
https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
Publisher Site
http://dx.doi.org/10.1186/s13008-019-0049-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570884PMC
June 2019
4 Reads

HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes.

Cell Div 2019 10;14. Epub 2019 May 10.

2Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 115 Taiwan.

Background: At the onset of mitosis, the centrosome expands and matures, acquiring enhanced activities for microtubule nucleation and assembly of a functional bipolar mitotic spindle. However, the mechanisms that regulate centrosome expansion and maturation are largely unknown. Previously, we demonstrated in an immortalized human cell line CGL2 and cancer cell line HeLa that the inducible form of heat shock protein 70 (HSP70) accumulates at the mitotic centrosome and is required for centrosome maturation and bipolar spindle assembly. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0047-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511203PMC
May 2019
1 Read

A novel resveratrol derivative induces mitotic arrest, centrosome fragmentation and cancer cell death by inhibiting γ-tubulin.

Cell Div 2019 10;14. Epub 2019 Apr 10.

1Department of Science, University of "Roma Tre", Rome, Italy.

Background: Resveratrol and its natural stilbene-containing derivatives have been extensively investigated as potential chemotherapeutic agents. The synthetic manipulation of the stilbene scaffold has led to the generation of new analogues with improved anticancer activity and better bioavailability. In the present study we investigated the anticancer activity of a novel trimethoxystilbene derivative (3,4,4'-trimethoxylstilbene), where two methoxyl groups are adjacent on the benzene ring (ortho configuration), and compared its activity to 3,5,4'-trimethoxylstilbene, whose methoxyl groups are in meta configuration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-019-0046-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457039PMC
April 2019
1 Read

p27kip1 at the crossroad between actin and microtubule dynamics.

Cell Div 2019 1;14. Epub 2019 Apr 1.

1Division of Molecular Oncology, Centro di Riferimento Oncologico di Aviano (CRO), IRCCS, National Cancer Institute, 33081 Aviano, Italy.

The p27 protein, mainly known as a negative regulator of cell proliferation, has also been involved in the control of other cellular processes, including the regulation of cytoskeleton dynamics. Notably, these two functions involve distinct protein domains, residing in the N- and C-terminal halves, respectively. In the last two decades, p27 has been reported to interact with microtubule and acto-myosin cytoskeletons, both in direct and indirect ways, overall drawing a picture in which several factors play their role either in synergy or in contrast one with another. Read More

View Article

Download full-text PDF

Source
https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
Publisher Site
http://dx.doi.org/10.1186/s13008-019-0045-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442415PMC
April 2019
13 Reads

Replication stress-induced Exo1 phosphorylation is mediated by Rad53/Pph3 and Exo1 nuclear localization is controlled by 14-3-3 proteins.

Cell Div 2019 4;14. Epub 2019 Jan 4.

Institute of Molecular Cancer Research, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Background: Mechanisms controlling DNA resection at sites of damage and affecting genome stability have been the subject of deep investigation, though their complexity is not yet fully understood. Specifically, the regulatory role of post-translational modifications in the localization, stability and function of DNA repair proteins is an important aspect of such complexity.

Results: Here, we took advantage of the superior resolution of phosphorylated proteins provided by Phos-Tag technology to study pathways controlling the reversible phosphorylation of yeast Exo1, an exonuclease involved in a number of DNA repair pathways. Read More

View Article

Download full-text PDF

Source
https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
Publisher Site
http://dx.doi.org/10.1186/s13008-018-0044-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318887PMC
January 2019
38 Reads

Reactivation of TAp73 tumor suppressor by protoporphyrin IX, a metabolite of aminolevulinic acid, induces apoptosis in 53-deficient cancer cells.

Cell Div 2018 26;13:10. Epub 2018 Dec 26.

2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Biomedicum, Solnavägen 9, 171 65 Stockholm, Sweden.

Background: The p73 protein is a tumor suppressor that shares structural and functional similarity with p53. p73 is expressed in two major isoforms; the TA isoform that interacts with p53 pathway, thus acting as tumor suppressor and the N-terminal truncated ΔN isoform that inhibits TAp73 and p53 and thus, acts as an oncogene.

Results: By employing a drug repurposing approach, we found that protoporphyrin IX (PpIX), a metabolite of aminolevulinic acid applied in photodynamic therapy of cancer, stabilizes TAp73 and activates TAp73-dependent apoptosis in cancer cells lacking p53. Read More

View Article

Download full-text PDF

Source
https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
Publisher Site
http://dx.doi.org/10.1186/s13008-018-0043-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306007PMC
December 2018
4 Reads

Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.

Cell Div 2018 10;13. Epub 2018 Nov 10.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.

Background: During oocyte meiosis, the cytoskeleton dynamics, especially spindle organization, are critical for chromosome congression and segregation. However, the roles of the kinesin superfamily in this process are still largely unknown.

Results: In the present study, Kif18a, a member of the kinesin-8 family, regulated spindle organization through its effects on tubulin acetylation in mouse oocyte meiosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0042-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234775PMC
November 2018
28 Reads

Triple A patient cells suffering from mitotic defects fail to localize PGRMC1 to mitotic kinetochore fibers.

Cell Div 2018 10;13. Epub 2018 Nov 10.

1Klinik und Poliklinik für Kinder- und Jugendmedizin, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.

Background: Membrane-associated progesterone receptors are restricted to the endoplasmic reticulum and are shown to regulate the activity of cytochrome P450 enzymes which are involved in steroidogenesis or drug detoxification. PGRMC1 and PGRMC2 belong to the membrane-associated progesterone receptor family and are of interest due to their suspected role during cell cycle. PGRMC1 and PGRMC2 are thought to bind to each other; thereby suppressing entry into mitosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0041-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230297PMC
November 2018
12 Reads

The functional diversity of Aurora kinases: a comprehensive review.

Cell Div 2018 19;13. Epub 2018 Sep 19.

1Laboratory of Nervous System Diseases and Therapy, GIGA-Neuroscience, University of Liège, Avenue Hippocrate, 15, 4000 Liège, Belgium.

Aurora kinases are serine/threonine kinases essential for the onset and progression of mitosis. Aurora members share a similar protein structure and kinase activity, but exhibit distinct cellular and subcellular localization. AurA favors the G2/M transition by promoting centrosome maturation and mitotic spindle assembly. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0040-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146527PMC
September 2018
4 Reads

Measuring DNA content in live cells by fluorescence microscopy.

Cell Div 2018 4;13. Epub 2018 Sep 4.

1University of Arizona Cancer Center, University of Arizona, 1515 N. Campbell Ave, Tucson, AZ 85724 USA.

Background: Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate cellular dynamics in real time. However, the capacity to simultaneously measure DNA content in cells being tracked over time remains challenged by dye-associated toxicities. The ability to measure DNA content in single cells by means of LCFM would allow cellular stage and ploidy to be coupled with a variety of imaging directed analyses. Read More

View Article

Download full-text PDF

Source
https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
Publisher Site
http://dx.doi.org/10.1186/s13008-018-0039-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123973PMC
September 2018
40 Reads

IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.

Cell Div 2018 15;13. Epub 2018 Jun 15.

1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT UK.

Background: Inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in de novo GTP biosynthesis, plays an important role in cell metabolism and proliferation. It has been demonstrated that IMPDH can aggregate into a macrostructure, termed the cytoophidium, in mammalian cells under a variety of conditions. However, the regulation and function of the cytoophidium are still elusive. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0038-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004095PMC
June 2018
39 Reads

Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement.

Cell Div 2018 24;13. Epub 2018 May 24.

3College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.

Background: Kinesin superfamily proteins are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. However, their exact roles during mammalian oocyte meiosis have not been fully clarified.

Results: Herein, we investigated the critical events during porcine oocyte meiotic maturation with the treatment of Eg5-specific inhibitor monastrol. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0037-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966870PMC
May 2018
8 Reads

Protein arginine methylation: an emerging regulator of the cell cycle.

Cell Div 2018 20;13. Epub 2018 Mar 20.

School of Science and Health, Western Sydney University, Penrith, NSW 2751 Australia.

Protein arginine methylation is a common post-translational modification where a methyl group is added onto arginine residues of a protein to alter detection by its binding partners or regulate its activity. It is known to be involved in many biological processes, such as regulation of signal transduction, transcription, facilitation of protein-protein interactions, RNA splicing and transport. The enzymes responsible for arginine methylation, protein arginine methyltransferases (PRMTs), have been shown to methylate or associate with important regulatory proteins of the cell cycle and DNA damage repair pathways, such as cyclin D1, p53, p21 and the retinoblastoma protein. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0036-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859524PMC
March 2018
8 Reads

Subunits of human condensins are potential therapeutic targets for cancers.

Cell Div 2018 20;13. Epub 2018 Feb 20.

3Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, K1N 6N5 Canada.

The main role of condensins is to regulate chromosome condensation and segregation during cell cycles. Recently, it has been suggested in the literatures that subunits of condensin I and condensin II are involved in some human cancers. This paper will first briefly discuss discoveries of human condensins, their components and structures, and their multiple cellular functions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0035-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819170PMC
February 2018
6 Reads

Phasing in on the cell cycle.

Cell Div 2018 25;13. Epub 2018 Jan 25.

1Department of Neurosciences, Experimental Neurology and Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven-University of Leuven, 3000 Leuven, Belgium.

Just like all matter, proteins can also switch between gas, liquid and solid phases. Protein phase transition has claimed the spotlight in recent years as a novel way of how cells compartmentalize and regulate biochemical reactions. Moreover, this discovery has provided a new framework for the study of membrane-less organelle biogenesis and protein aggregation in neurodegenerative disorders. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-018-0034-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785872PMC
January 2018
5 Reads

The emerging roles of CDK12 in tumorigenesis.

Cell Div 2017 27;12. Epub 2017 Oct 27.

Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 296/70, Brno, 621 00 Czech Republic.

Cyclin-dependent kinases (CDKs) are key regulators of both cell cycle progression and transcription. Since dysregulation of CDKs is a frequently occurring event driving tumorigenesis, CDKs have been tested extensively as targets for cancer therapy. Cyclin-dependent kinase 12 (CDK12) is a transcription-associated kinase which participates in various cellular processes, including DNA damage response, development and cellular differentiation, as well as splicing and pre-mRNA processing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-017-0033-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658942PMC
October 2017
11 Reads

Proteins associated with the doubling time of the NCI-60 cancer cell lines.

Cell Div 2017 29;12. Epub 2017 Aug 29.

Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843 USA.

The varied nature of human cancers is recapitulated, at least to some extent, in the diverse NCI-60 panel of human cancer cell lines. Here, I used a basic, continuous variable of proliferating cells, their doubling time, to stratify the proteome across the NCI-60 cell lines. Among >7000 proteins quantified in the NCI-60 panel previously, the levels of 84 proteins increase in cells that proliferate slowly. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-017-0032-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574231PMC
August 2017
7 Reads

Erratum to: Rpl22 is required for mRNA translation and meiotic induction in .

Cell Div 2017 18;12. Epub 2017 Jul 18.

Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Two Medical Center Dr., Stratford, NJ 08055 USA.

[This corrects the article DOI: 10.1186/s13008-016-0024-3.]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-017-0031-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516299PMC
July 2017
15 Reads

Erratum to: Electrostatic forces drive poleward chromosome motions at kinetochores.

Cell Div 2017 3;12. Epub 2017 Jul 3.

Departments of Physics and Biology, Rutgers The State University of New Jersey, Camden, NJ 08102 USA.

[This corrects the article DOI: 10.1186/s13008-016-0026-1.]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-017-0030-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496239PMC
July 2017
4 Reads

Multiple molecular interactions redundantly contribute to RB-mediated cell cycle control.

Cell Div 2017 14;12. Epub 2017 Mar 14.

London Regional Cancer Program, London, Canada.

Background: The G1-S phase transition is critical to maintaining proliferative control and preventing carcinogenesis. The retinoblastoma tumor suppressor is a key regulator of this step in the cell cycle.

Results: Here we use a structure-function approach to evaluate the contributions of multiple protein interaction surfaces on pRB towards cell cycle regulation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-017-0029-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348811PMC
March 2017
17 Reads

Systematic epistatic mapping of cellular processes.

Cell Div 2017 6;12. Epub 2017 Jan 6.

German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics and Heidelberg University, Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany ; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany.

Genetic screens have identified many novel components of various biological processes, such as components required for cell cycle and cell division. While forward genetic screens typically generate unstructured 'hit' lists, genetic interaction mapping approaches can identify functional relations in a systematic fashion. Here, we discuss a recent study by our group demonstrating a two-step approach to first screen for regulators of the mitotic cell cycle, and subsequently guide hypothesis generation by using genetic interaction analysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0028-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223360PMC
January 2017
10 Reads

The Irr1/Scc3 protein implicated in chromosome segregation in has a dual nuclear-cytoplasmic localization.

Cell Div 2017 3;12. Epub 2017 Jan 3.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland.

Background: Correct chromosome segregation depends on the sister chromatid cohesion complex. The essential, evolutionarily conserved regulatory protein Irr1/Scc3, is responsible for the complex loading onto DNA and for its removal. We found that, unexpectedly, Irr1 is present not only in the nucleus but also in the cytoplasm. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0027-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223379PMC
January 2017
8 Reads

Electrostatic forces drive poleward chromosome motions at kinetochores.

Cell Div 2016 28;11:14. Epub 2016 Oct 28.

Departments of Physics and Biology, Rutgers The State University of New Jersey, Camden, NJ 08102 USA.

Background: Recent experiments regarding Ndc80/Hec1 in force generation at kinetochores for chromosome motions have prompted speculation about possible models for interactions between positively charged molecules at kinetochores and negative charge at and near the plus ends of microtubules.

Discussion: A clear picture of how kinetochores and centrosomes establish and maintain a dynamic coupling to microtubules for force generation during the complex motions of mitosis remains elusive. The current paradigm of molecular cell biology requires that specific molecules, or molecular geometries, for force generation be identified. Read More

View Article

Download full-text PDF

Source
http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
Publisher Site
http://dx.doi.org/10.1186/s13008-016-0026-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086063PMC
October 2016
21 Reads

Erratum to: The loop-less Cdc34 E2 mutant defective polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2.

Cell Div 2016 6;11:13. Epub 2016 Oct 6.

Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO 63104 USA.

[This corrects the article DOI: 10.1186/1747-1028-6-7.]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0018-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054535PMC
October 2016
32 Reads

The CSL proteins, versatile transcription factors and context dependent corepressors of the notch signaling pathway.

Cell Div 2016 27;11:12. Epub 2016 Sep 27.

Centro Multidisciplinario de Estudios en Biotecnología (CMEB) de la Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Posta Veterinaria, Km. 9.5 Carretera Morelia-Zinapécuaro, Col. La Palma, C. P. 58890 Tarímbaro, Mich. México.

The Notch signaling pathway is a reiteratively used cell to cell communication pathway that triggers pleiotropic effects. The correct regulation of the pathway permits the efficient regulation of genes involved in cell fate decision throughout development. This activity relies notably on the CSL proteins, (an acronym for BF-1/RBPJ-κ in / respectively, uppressor of Hairless in , ag-1 in ) which is the unique transcription factor and DNA binding protein involved in this pathway. Read More

View Article

Download full-text PDF

Source
http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
Publisher Site
http://dx.doi.org/10.1186/s13008-016-0025-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037638PMC
September 2016
27 Reads

Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways.

Cell Div 2016 2;11:11. Epub 2016 Aug 2.

Department of Pathology, University of Mississippi Medical Center, Jackson, MS 39216 USA ; Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, Kirrberger Str. 100, 66421 Homburg/Saar, Germany ; Institut National de la Santé et de la Recherche Médicale, University of Strasbourg, 67000 Strasbourg, France ; Cancer Institute, University of Mississippi Medical Center, Jackson, MS 39216 USA.

Background: Tumor response to immunotherapy is the consequence of a concerted crosstalk between cytokines and effector cells. Interferon gamma (IFNγ) is one of the common cytokines coordinating tumor immune response and the associated biological consequences. Although the role of IFNγ in the modulation of tumor immunity has been widely documented, the mechanisms regulating IFNγ-induced cell death, during the course of immune therapy, is not described in detail. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0023-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969639PMC
August 2016
51 Reads

Rpl22 is required for IME1 mRNA translation and meiotic induction in S. cerevisiae.

Cell Div 2016 29;11:10. Epub 2016 Jul 29.

Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Two Medical Center Dr., Stratford, NJ 08055 USA.

Background: The transition from mitotic cell division to meiotic development in S. cerevisiae requires induction of a transient transcription program that is initiated by Ime1-dependent destruction of the repressor Ume6. Although IME1 mRNA is observed in vegetative cultures, Ime1 protein is not suggesting the presence of a regulatory system restricting translation to meiotic cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0024-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966820PMC
August 2016
18 Reads

Insights into APC/C: from cellular function to diseases and therapeutics.

Cell Div 2016 13;11. Epub 2016 Jul 13.

Department of Cell Biology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, 5117 Centre Avenue, Hillman Cancer Center, HCC2.6c, Pittsburgh, PA 15213 USA.

Anaphase-promoting complex/cyclosome (APC/C) is a multifunctional ubiquitin-protein ligase that targets different substrates for ubiquitylation and therefore regulates a variety of cellular processes such as cell division, differentiation, genome stability, energy metabolism, cell death, autophagy as well as carcinogenesis. Activity of APC/C is principally governed by two WD-40 domain proteins, Cdc20 and Cdh1, in and beyond cell cycle. In the past decade, the results based on numerous biochemical, 3D structural, mouse genetic and small molecule inhibitor studies have largely attracted our attention into the emerging role of APC/C and its regulation in biological function, human diseases and potential therapeutics. Read More

View Article

Download full-text PDF

Source
http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
Publisher Site
http://dx.doi.org/10.1186/s13008-016-0021-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944252PMC
July 2016
12 Reads

Cullin-RING ligases in regulation of autophagy.

Cell Div 2016 10;11. Epub 2016 Jun 10.

Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qing-Chun Road, Hangzhou, Zhejiang 310003 People's Republic of China ; Institute of Translational Medicine, Zhejiang University School of Medicine, 268 Kai-Xuan Road, Hangzhou, Zhejiang 310029 People's Republic of China.

Cullin-RING ligases (CRLs), the largest E3 ubiquitin ligase family, promote ubiquitination and degradation of various cellular key regulators involved in a broad array of physiological and pathological processes, including cell cycle progression, signal transduction, transcription, cardiomyopathy, and tumorigenesis. Autophagy, an intracellular catabolic reaction that delivers cytoplasmic components to lysosomes for degradation, is crucial for cellular metabolism and homeostasis. The dysfunction of autophagy has been proved to associate with a variety of human diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0022-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902950PMC
June 2016
49 Reads

The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions.

Cell Div 2016 23;11. Epub 2016 May 23.

Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 USA.

Background: Cullin-RING E3 ubiquitin ligase complexes play a central role in targeting cellular proteins for ubiquitination-dependent protein turnover through 26S proteasome. Cullin-2 is a member of the Cullin family, and it serves as a scaffold protein for Elongin B and C, Rbx1 and various substrate recognition receptors to form E3 ubiquitin ligases.

Main Body Of The Abstract: First, the composition, structure and the regulation of Cullin-2 based E3 ubiquitin ligases were introduced. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0020-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878042PMC
May 2016
20 Reads

The T-box transcription factor TBX3 drives proliferation by direct repression of the p21(WAF1) cyclin-dependent kinase inhibitor.

Cell Div 2016 22;11. Epub 2016 Apr 22.

Division of Cell Biology, Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925 South Africa.

Background: TBX3, a member of the T-box family of transcription factors, is essential in development and has emerged as an important player in the oncogenic process. TBX3 is overexpressed in several cancers and has been shown to contribute directly to tumour formation, migration and invasion. However, little is known about the molecular basis for its role in development and oncogenesis because there is a paucity of information regarding its target genes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13008-016-0019-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840944PMC
April 2016
37 Reads

Cul3-KLHL20 ubiquitin ligase: physiological functions, stress responses, and disease implications.

Cell Div 2016 1;11. Epub 2016 Apr 1.

Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan ; Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan.

Cullin-RING ubiquitin ligases are the largest Ubiquitin ligase family in eukaryotes and are multi-protein complexes. In these complexes, the Cullin protein serves as a scaffold to connect two functional modules of the ligases, the catalytic subunit and substrate-binding subunit. KLHL20 is a substrate-binding subunit of Cullin3 (Cul3) ubiquitin ligase. Read More

View Article

Download full-text PDF

Source
http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
Publisher Site
http://dx.doi.org/10.1186/s13008-016-0017-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818519PMC
April 2016
15 Reads