231 results match your criteria Cell Division [Journal]


A novel resveratrol derivative induces mitotic arrest, centrosome fragmentation and cancer cell death by inhibiting γ-tubulin.

Cell Div 2019 10;14. Epub 2019 Apr 10.

1Department of Science, University of "Roma Tre", Rome, Italy.

Background: Resveratrol and its natural stilbene-containing derivatives have been extensively investigated as potential chemotherapeutic agents. The synthetic manipulation of the stilbene scaffold has led to the generation of new analogues with improved anticancer activity and better bioavailability. In the present study we investigated the anticancer activity of a novel trimethoxystilbene derivative (3,4,4'-trimethoxylstilbene), where two methoxyl groups are adjacent on the benzene ring (ortho configuration), and compared its activity to 3,5,4'-trimethoxylstilbene, whose methoxyl groups are in meta configuration. Read More

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http://dx.doi.org/10.1186/s13008-019-0046-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457039PMC

p27kip1 at the crossroad between actin and microtubule dynamics.

Cell Div 2019 1;14. Epub 2019 Apr 1.

1Division of Molecular Oncology, Centro di Riferimento Oncologico di Aviano (CRO), IRCCS, National Cancer Institute, 33081 Aviano, Italy.

The p27 protein, mainly known as a negative regulator of cell proliferation, has also been involved in the control of other cellular processes, including the regulation of cytoskeleton dynamics. Notably, these two functions involve distinct protein domains, residing in the N- and C-terminal halves, respectively. In the last two decades, p27 has been reported to interact with microtubule and acto-myosin cytoskeletons, both in direct and indirect ways, overall drawing a picture in which several factors play their role either in synergy or in contrast one with another. Read More

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https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
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http://dx.doi.org/10.1186/s13008-019-0045-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442415PMC
April 2019
1 Read

Replication stress-induced Exo1 phosphorylation is mediated by Rad53/Pph3 and Exo1 nuclear localization is controlled by 14-3-3 proteins.

Cell Div 2019 4;14. Epub 2019 Jan 4.

Institute of Molecular Cancer Research, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Background: Mechanisms controlling DNA resection at sites of damage and affecting genome stability have been the subject of deep investigation, though their complexity is not yet fully understood. Specifically, the regulatory role of post-translational modifications in the localization, stability and function of DNA repair proteins is an important aspect of such complexity.

Results: Here, we took advantage of the superior resolution of phosphorylated proteins provided by Phos-Tag technology to study pathways controlling the reversible phosphorylation of yeast Exo1, an exonuclease involved in a number of DNA repair pathways. Read More

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https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
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http://dx.doi.org/10.1186/s13008-018-0044-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318887PMC
January 2019
20 Reads

Reactivation of TAp73 tumor suppressor by protoporphyrin IX, a metabolite of aminolevulinic acid, induces apoptosis in 53-deficient cancer cells.

Cell Div 2018 26;13:10. Epub 2018 Dec 26.

2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Biomedicum, Solnavägen 9, 171 65 Stockholm, Sweden.

Background: The p73 protein is a tumor suppressor that shares structural and functional similarity with p53. p73 is expressed in two major isoforms; the TA isoform that interacts with p53 pathway, thus acting as tumor suppressor and the N-terminal truncated ΔN isoform that inhibits TAp73 and p53 and thus, acts as an oncogene.

Results: By employing a drug repurposing approach, we found that protoporphyrin IX (PpIX), a metabolite of aminolevulinic acid applied in photodynamic therapy of cancer, stabilizes TAp73 and activates TAp73-dependent apoptosis in cancer cells lacking p53. Read More

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https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
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http://dx.doi.org/10.1186/s13008-018-0043-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306007PMC
December 2018
2 Reads

Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.

Cell Div 2018 10;13. Epub 2018 Nov 10.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.

Background: During oocyte meiosis, the cytoskeleton dynamics, especially spindle organization, are critical for chromosome congression and segregation. However, the roles of the kinesin superfamily in this process are still largely unknown.

Results: In the present study, Kif18a, a member of the kinesin-8 family, regulated spindle organization through its effects on tubulin acetylation in mouse oocyte meiosis. Read More

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http://dx.doi.org/10.1186/s13008-018-0042-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234775PMC
November 2018
15 Reads

Triple A patient cells suffering from mitotic defects fail to localize PGRMC1 to mitotic kinetochore fibers.

Cell Div 2018 10;13. Epub 2018 Nov 10.

1Klinik und Poliklinik für Kinder- und Jugendmedizin, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.

Background: Membrane-associated progesterone receptors are restricted to the endoplasmic reticulum and are shown to regulate the activity of cytochrome P450 enzymes which are involved in steroidogenesis or drug detoxification. PGRMC1 and PGRMC2 belong to the membrane-associated progesterone receptor family and are of interest due to their suspected role during cell cycle. PGRMC1 and PGRMC2 are thought to bind to each other; thereby suppressing entry into mitosis. Read More

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http://dx.doi.org/10.1186/s13008-018-0041-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230297PMC
November 2018
3 Reads

The functional diversity of Aurora kinases: a comprehensive review.

Cell Div 2018 19;13. Epub 2018 Sep 19.

1Laboratory of Nervous System Diseases and Therapy, GIGA-Neuroscience, University of Liège, Avenue Hippocrate, 15, 4000 Liège, Belgium.

Aurora kinases are serine/threonine kinases essential for the onset and progression of mitosis. Aurora members share a similar protein structure and kinase activity, but exhibit distinct cellular and subcellular localization. AurA favors the G2/M transition by promoting centrosome maturation and mitotic spindle assembly. Read More

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http://dx.doi.org/10.1186/s13008-018-0040-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146527PMC
September 2018
1 Read

Measuring DNA content in live cells by fluorescence microscopy.

Cell Div 2018 4;13. Epub 2018 Sep 4.

1University of Arizona Cancer Center, University of Arizona, 1515 N. Campbell Ave, Tucson, AZ 85724 USA.

Background: Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate cellular dynamics in real time. However, the capacity to simultaneously measure DNA content in cells being tracked over time remains challenged by dye-associated toxicities. The ability to measure DNA content in single cells by means of LCFM would allow cellular stage and ploidy to be coupled with a variety of imaging directed analyses. Read More

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https://celldiv.biomedcentral.com/articles/10.1186/s13008-01
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http://dx.doi.org/10.1186/s13008-018-0039-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123973PMC
September 2018
22 Reads

IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.

Cell Div 2018 15;13. Epub 2018 Jun 15.

1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT UK.

Background: Inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in de novo GTP biosynthesis, plays an important role in cell metabolism and proliferation. It has been demonstrated that IMPDH can aggregate into a macrostructure, termed the cytoophidium, in mammalian cells under a variety of conditions. However, the regulation and function of the cytoophidium are still elusive. Read More

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http://dx.doi.org/10.1186/s13008-018-0038-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004095PMC
June 2018
25 Reads

Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement.

Cell Div 2018 24;13. Epub 2018 May 24.

3College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.

Background: Kinesin superfamily proteins are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. However, their exact roles during mammalian oocyte meiosis have not been fully clarified.

Results: Herein, we investigated the critical events during porcine oocyte meiotic maturation with the treatment of Eg5-specific inhibitor monastrol. Read More

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http://dx.doi.org/10.1186/s13008-018-0037-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966870PMC
May 2018
5 Reads

Protein arginine methylation: an emerging regulator of the cell cycle.

Cell Div 2018 20;13. Epub 2018 Mar 20.

School of Science and Health, Western Sydney University, Penrith, NSW 2751 Australia.

Protein arginine methylation is a common post-translational modification where a methyl group is added onto arginine residues of a protein to alter detection by its binding partners or regulate its activity. It is known to be involved in many biological processes, such as regulation of signal transduction, transcription, facilitation of protein-protein interactions, RNA splicing and transport. The enzymes responsible for arginine methylation, protein arginine methyltransferases (PRMTs), have been shown to methylate or associate with important regulatory proteins of the cell cycle and DNA damage repair pathways, such as cyclin D1, p53, p21 and the retinoblastoma protein. Read More

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http://dx.doi.org/10.1186/s13008-018-0036-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859524PMC
March 2018
7 Reads

Subunits of human condensins are potential therapeutic targets for cancers.

Cell Div 2018 20;13. Epub 2018 Feb 20.

3Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, K1N 6N5 Canada.

The main role of condensins is to regulate chromosome condensation and segregation during cell cycles. Recently, it has been suggested in the literatures that subunits of condensin I and condensin II are involved in some human cancers. This paper will first briefly discuss discoveries of human condensins, their components and structures, and their multiple cellular functions. Read More

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http://dx.doi.org/10.1186/s13008-018-0035-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819170PMC
February 2018
5 Reads

Phasing in on the cell cycle.

Cell Div 2018 25;13. Epub 2018 Jan 25.

1Department of Neurosciences, Experimental Neurology and Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven-University of Leuven, 3000 Leuven, Belgium.

Just like all matter, proteins can also switch between gas, liquid and solid phases. Protein phase transition has claimed the spotlight in recent years as a novel way of how cells compartmentalize and regulate biochemical reactions. Moreover, this discovery has provided a new framework for the study of membrane-less organelle biogenesis and protein aggregation in neurodegenerative disorders. Read More

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http://dx.doi.org/10.1186/s13008-018-0034-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785872PMC
January 2018
4 Reads

The emerging roles of CDK12 in tumorigenesis.

Cell Div 2017 27;12. Epub 2017 Oct 27.

Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 296/70, Brno, 621 00 Czech Republic.

Cyclin-dependent kinases (CDKs) are key regulators of both cell cycle progression and transcription. Since dysregulation of CDKs is a frequently occurring event driving tumorigenesis, CDKs have been tested extensively as targets for cancer therapy. Cyclin-dependent kinase 12 (CDK12) is a transcription-associated kinase which participates in various cellular processes, including DNA damage response, development and cellular differentiation, as well as splicing and pre-mRNA processing. Read More

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http://dx.doi.org/10.1186/s13008-017-0033-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658942PMC
October 2017
10 Reads

Proteins associated with the doubling time of the NCI-60 cancer cell lines.

Cell Div 2017 29;12. Epub 2017 Aug 29.

Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843 USA.

The varied nature of human cancers is recapitulated, at least to some extent, in the diverse NCI-60 panel of human cancer cell lines. Here, I used a basic, continuous variable of proliferating cells, their doubling time, to stratify the proteome across the NCI-60 cell lines. Among >7000 proteins quantified in the NCI-60 panel previously, the levels of 84 proteins increase in cells that proliferate slowly. Read More

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http://dx.doi.org/10.1186/s13008-017-0032-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574231PMC
August 2017
6 Reads

Erratum to: Rpl22 is required for mRNA translation and meiotic induction in .

Cell Div 2017 18;12. Epub 2017 Jul 18.

Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Two Medical Center Dr., Stratford, NJ 08055 USA.

[This corrects the article DOI: 10.1186/s13008-016-0024-3.]. Read More

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http://dx.doi.org/10.1186/s13008-017-0031-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516299PMC
July 2017
8 Reads

Erratum to: Electrostatic forces drive poleward chromosome motions at kinetochores.

Cell Div 2017 3;12. Epub 2017 Jul 3.

Departments of Physics and Biology, Rutgers The State University of New Jersey, Camden, NJ 08102 USA.

[This corrects the article DOI: 10.1186/s13008-016-0026-1.]. Read More

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http://dx.doi.org/10.1186/s13008-017-0030-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496239PMC
July 2017
4 Reads

Multiple molecular interactions redundantly contribute to RB-mediated cell cycle control.

Cell Div 2017 14;12. Epub 2017 Mar 14.

London Regional Cancer Program, London, Canada.

Background: The G1-S phase transition is critical to maintaining proliferative control and preventing carcinogenesis. The retinoblastoma tumor suppressor is a key regulator of this step in the cell cycle.

Results: Here we use a structure-function approach to evaluate the contributions of multiple protein interaction surfaces on pRB towards cell cycle regulation. Read More

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http://dx.doi.org/10.1186/s13008-017-0029-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348811PMC
March 2017
10 Reads

Systematic epistatic mapping of cellular processes.

Cell Div 2017 6;12. Epub 2017 Jan 6.

German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics and Heidelberg University, Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany ; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany.

Genetic screens have identified many novel components of various biological processes, such as components required for cell cycle and cell division. While forward genetic screens typically generate unstructured 'hit' lists, genetic interaction mapping approaches can identify functional relations in a systematic fashion. Here, we discuss a recent study by our group demonstrating a two-step approach to first screen for regulators of the mitotic cell cycle, and subsequently guide hypothesis generation by using genetic interaction analysis. Read More

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http://dx.doi.org/10.1186/s13008-016-0028-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223360PMC
January 2017
9 Reads

The Irr1/Scc3 protein implicated in chromosome segregation in has a dual nuclear-cytoplasmic localization.

Cell Div 2017 3;12. Epub 2017 Jan 3.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland.

Background: Correct chromosome segregation depends on the sister chromatid cohesion complex. The essential, evolutionarily conserved regulatory protein Irr1/Scc3, is responsible for the complex loading onto DNA and for its removal. We found that, unexpectedly, Irr1 is present not only in the nucleus but also in the cytoplasm. Read More

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http://dx.doi.org/10.1186/s13008-016-0027-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223379PMC
January 2017
7 Reads

Electrostatic forces drive poleward chromosome motions at kinetochores.

Cell Div 2016 28;11:14. Epub 2016 Oct 28.

Departments of Physics and Biology, Rutgers The State University of New Jersey, Camden, NJ 08102 USA.

Background: Recent experiments regarding Ndc80/Hec1 in force generation at kinetochores for chromosome motions have prompted speculation about possible models for interactions between positively charged molecules at kinetochores and negative charge at and near the plus ends of microtubules.

Discussion: A clear picture of how kinetochores and centrosomes establish and maintain a dynamic coupling to microtubules for force generation during the complex motions of mitosis remains elusive. The current paradigm of molecular cell biology requires that specific molecules, or molecular geometries, for force generation be identified. Read More

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http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
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http://dx.doi.org/10.1186/s13008-016-0026-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086063PMC
October 2016
14 Reads

Erratum to: The loop-less Cdc34 E2 mutant defective polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2.

Cell Div 2016 6;11:13. Epub 2016 Oct 6.

Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO 63104 USA.

[This corrects the article DOI: 10.1186/1747-1028-6-7.]. Read More

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http://dx.doi.org/10.1186/s13008-016-0018-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054535PMC
October 2016
19 Reads

The CSL proteins, versatile transcription factors and context dependent corepressors of the notch signaling pathway.

Cell Div 2016 27;11:12. Epub 2016 Sep 27.

Centro Multidisciplinario de Estudios en Biotecnología (CMEB) de la Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Posta Veterinaria, Km. 9.5 Carretera Morelia-Zinapécuaro, Col. La Palma, C. P. 58890 Tarímbaro, Mich. México.

The Notch signaling pathway is a reiteratively used cell to cell communication pathway that triggers pleiotropic effects. The correct regulation of the pathway permits the efficient regulation of genes involved in cell fate decision throughout development. This activity relies notably on the CSL proteins, (an acronym for BF-1/RBPJ-κ in / respectively, uppressor of Hairless in , ag-1 in ) which is the unique transcription factor and DNA binding protein involved in this pathway. Read More

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http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
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http://dx.doi.org/10.1186/s13008-016-0025-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037638PMC
September 2016
18 Reads

Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways.

Cell Div 2016 2;11:11. Epub 2016 Aug 2.

Department of Pathology, University of Mississippi Medical Center, Jackson, MS 39216 USA ; Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, Kirrberger Str. 100, 66421 Homburg/Saar, Germany ; Institut National de la Santé et de la Recherche Médicale, University of Strasbourg, 67000 Strasbourg, France ; Cancer Institute, University of Mississippi Medical Center, Jackson, MS 39216 USA.

Background: Tumor response to immunotherapy is the consequence of a concerted crosstalk between cytokines and effector cells. Interferon gamma (IFNγ) is one of the common cytokines coordinating tumor immune response and the associated biological consequences. Although the role of IFNγ in the modulation of tumor immunity has been widely documented, the mechanisms regulating IFNγ-induced cell death, during the course of immune therapy, is not described in detail. Read More

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http://dx.doi.org/10.1186/s13008-016-0023-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969639PMC
August 2016
27 Reads

Rpl22 is required for IME1 mRNA translation and meiotic induction in S. cerevisiae.

Cell Div 2016 29;11:10. Epub 2016 Jul 29.

Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Two Medical Center Dr., Stratford, NJ 08055 USA.

Background: The transition from mitotic cell division to meiotic development in S. cerevisiae requires induction of a transient transcription program that is initiated by Ime1-dependent destruction of the repressor Ume6. Although IME1 mRNA is observed in vegetative cultures, Ime1 protein is not suggesting the presence of a regulatory system restricting translation to meiotic cells. Read More

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http://dx.doi.org/10.1186/s13008-016-0024-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966820PMC
August 2016
11 Reads

Insights into APC/C: from cellular function to diseases and therapeutics.

Cell Div 2016 13;11. Epub 2016 Jul 13.

Department of Cell Biology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, 5117 Centre Avenue, Hillman Cancer Center, HCC2.6c, Pittsburgh, PA 15213 USA.

Anaphase-promoting complex/cyclosome (APC/C) is a multifunctional ubiquitin-protein ligase that targets different substrates for ubiquitylation and therefore regulates a variety of cellular processes such as cell division, differentiation, genome stability, energy metabolism, cell death, autophagy as well as carcinogenesis. Activity of APC/C is principally governed by two WD-40 domain proteins, Cdc20 and Cdh1, in and beyond cell cycle. In the past decade, the results based on numerous biochemical, 3D structural, mouse genetic and small molecule inhibitor studies have largely attracted our attention into the emerging role of APC/C and its regulation in biological function, human diseases and potential therapeutics. Read More

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http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
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http://dx.doi.org/10.1186/s13008-016-0021-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944252PMC
July 2016
9 Reads

Cullin-RING ligases in regulation of autophagy.

Cell Div 2016 10;11. Epub 2016 Jun 10.

Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qing-Chun Road, Hangzhou, Zhejiang 310003 People's Republic of China ; Institute of Translational Medicine, Zhejiang University School of Medicine, 268 Kai-Xuan Road, Hangzhou, Zhejiang 310029 People's Republic of China.

Cullin-RING ligases (CRLs), the largest E3 ubiquitin ligase family, promote ubiquitination and degradation of various cellular key regulators involved in a broad array of physiological and pathological processes, including cell cycle progression, signal transduction, transcription, cardiomyopathy, and tumorigenesis. Autophagy, an intracellular catabolic reaction that delivers cytoplasmic components to lysosomes for degradation, is crucial for cellular metabolism and homeostasis. The dysfunction of autophagy has been proved to associate with a variety of human diseases. Read More

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http://dx.doi.org/10.1186/s13008-016-0022-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902950PMC
June 2016
38 Reads

The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions.

Cell Div 2016 23;11. Epub 2016 May 23.

Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 USA.

Background: Cullin-RING E3 ubiquitin ligase complexes play a central role in targeting cellular proteins for ubiquitination-dependent protein turnover through 26S proteasome. Cullin-2 is a member of the Cullin family, and it serves as a scaffold protein for Elongin B and C, Rbx1 and various substrate recognition receptors to form E3 ubiquitin ligases.

Main Body Of The Abstract: First, the composition, structure and the regulation of Cullin-2 based E3 ubiquitin ligases were introduced. Read More

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http://dx.doi.org/10.1186/s13008-016-0020-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878042PMC
May 2016
17 Reads

The T-box transcription factor TBX3 drives proliferation by direct repression of the p21(WAF1) cyclin-dependent kinase inhibitor.

Cell Div 2016 22;11. Epub 2016 Apr 22.

Division of Cell Biology, Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925 South Africa.

Background: TBX3, a member of the T-box family of transcription factors, is essential in development and has emerged as an important player in the oncogenic process. TBX3 is overexpressed in several cancers and has been shown to contribute directly to tumour formation, migration and invasion. However, little is known about the molecular basis for its role in development and oncogenesis because there is a paucity of information regarding its target genes. Read More

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http://dx.doi.org/10.1186/s13008-016-0019-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840944PMC
April 2016
18 Reads

Cul3-KLHL20 ubiquitin ligase: physiological functions, stress responses, and disease implications.

Cell Div 2016 1;11. Epub 2016 Apr 1.

Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan ; Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan.

Cullin-RING ubiquitin ligases are the largest Ubiquitin ligase family in eukaryotes and are multi-protein complexes. In these complexes, the Cullin protein serves as a scaffold to connect two functional modules of the ligases, the catalytic subunit and substrate-binding subunit. KLHL20 is a substrate-binding subunit of Cullin3 (Cul3) ubiquitin ligase. Read More

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http://celldiv.biomedcentral.com/articles/10.1186/s13008-016
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http://dx.doi.org/10.1186/s13008-016-0017-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818519PMC
April 2016
12 Reads

New insights into posttranslational modifications of Hippo pathway in carcinogenesis and therapeutics.

Cell Div 2016 31;11. Epub 2016 Mar 31.

Department of Cell Biology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Hillman Cancer Center, 5117 Centre Avenue, HCC2.6c, Pittsburgh, PA 15213 USA.

PTMs (posttranslational modifications) such as ubiquitylation, sumoylation, acetylation and protein methylation are pivotal modifiers that determine the activation, deactivation or subcellular localization of signaling proteins, facilitating the initiation, amplification and transduction of signaling. Accumulating evidence suggest that several key signaling molecules in Hippo signaling pathway are tightly regulated by various types of PTMs. Malfunction of these critical signaling modules such as YAP/TAZ, MAT1/2 and LATS1/2 due to deregulated PTMs has been linked to a variety of human diseases such as cancer. Read More

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http://dx.doi.org/10.1186/s13008-016-0013-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4818516PMC
April 2016
8 Reads

Mitochondria-cytoskeleton associations in mammalian cytokinesis.

Cell Div 2016 18;11. Epub 2016 Mar 18.

Department of Anatomy and Cell Biology, McGill University, Montreal, QC Canada.

Background: The role of the cytoskeleton in regulating mitochondrial distribution in dividing mammalian cells is poorly understood. We previously demonstrated that mitochondria are transported to the cleavage furrow during cytokinesis in a microtubule-dependent manner. However, the exact subset of spindle microtubules and molecular machinery involved remains unknown. Read More

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http://dx.doi.org/10.1186/s13008-016-0015-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812650PMC
March 2016
6 Reads

C1D family proteins in coordinating RNA processing, chromosome condensation and DNA damage response.

Cell Div 2016 9;11. Epub 2016 Mar 9.

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597 Singapore ; National University Health System (NUHS), Singapore, 119228 Singapore ; NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, 119228 Singapore.

Research on the involvement of C1D and its yeast homologues Rrp47 (S. cerevisiae) and Cti1 (S. pombe) in DNA damage repair and RNA processing has remained mutually exclusive, with most studies predominantly concentrating on Rrp47. Read More

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http://dx.doi.org/10.1186/s13008-016-0014-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812661PMC
March 2016
9 Reads

The role of cullin 5-containing ubiquitin ligases.

Cell Div 2016 9;11. Epub 2016 Mar 9.

Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602 Japan.

The suppressor of cytokine signaling (SOCS) box consists of the BC box and the cullin 5 (Cul5) box, which interact with Elongin BC and Cul5, respectively. SOCS box-containing proteins have ubiquitin ligase activity mediated by the formation of a complex with the scaffold protein Cul5 and the RING domain protein Rbx2, and are thereby members of the cullin RING ligase superfamily. Cul5-type ubiquitin ligases have a variety of substrates that are targeted for polyubiquitination and proteasomal degradation. Read More

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http://dx.doi.org/10.1186/s13008-016-0016-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812663PMC
March 2016
16 Reads

Curcumin and tumor immune-editing: resurrecting the immune system.

Cell Div 2015 12;10. Epub 2015 Oct 12.

Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata, 700054 India.

Curcumin has long been known to posses medicinal properties and recent scientific studies have shown its efficacy in treating cancer. Curcumin is now considered to be a promising anti-cancer agent and studies continue on its molecular mechanism of action. Curcumin has been shown to act in a multi-faceted manner by targeting the classical hallmarks of cancer like sustained proliferation, evasion of apoptosis, sustained angiogenesis, insensitivity to growth inhibitors, tissue invasion and metastasis etc. Read More

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http://dx.doi.org/10.1186/s13008-015-0012-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603973PMC
October 2015
15 Reads
13 Citations
3.530 Impact Factor

COP9-Signalosome deneddylase activity is enhanced by simultaneous neddylation: insights into the regulation of an enzymatic protein complex.

Cell Div 2015 11;10. Epub 2015 Aug 11.

The Rheumatology Unit, The Chaim Sheba Medical Center, Tel-Hashomer, affiliated with the Sackler Faculty of Medicine, Tel-Aviv University, Tel Hashomer, Israel.

Background: Cullin-RING ubiquitin ligases (CRLs) are regulated by neddylation, which is a post translation modification of the Cullin family proteins. Neddylation of Cul1 activates the ligase through some means of biochemical mechanisms. The rate of neddylation and its extent are regulated by 2 opposing enzymatic processes: neddylation by an enzymatic cascade, and deneddylation by COP9-Signalosome (CSN) complex protein. Read More

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http://dx.doi.org/10.1186/s13008-015-0011-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531434PMC
August 2015
6 Reads

Sumo and the cellular stress response.

Cell Div 2015 20;10. Epub 2015 Jun 20.

Institute for Microbiology, Oslo University Hospital, Sognsvannsveien 20N-0027, Oslo, Norway.

The ubiquitin family member Sumo has important functions in many cellular processes including DNA repair, transcription and cell division. Numerous studies have shown that Sumo is essential for maintaining cell homeostasis when the cell encounters endogenous or environmental stress, such as osmotic stress, hypoxia, heat shock, genotoxic stress, and nutrient stress. Regulation of transcription is a key component of the Sumo stress response, and multiple mechanisms have been described by which Sumo can regulate transcription. Read More

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http://dx.doi.org/10.1186/s13008-015-0010-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476178PMC
June 2015
8 Reads

Aneuploidy and chromosomal instability in cancer: a jackpot to chaos.

Cell Div 2015 20;10. Epub 2015 May 20.

Institute for Medical Biology (IMB), Agency for Science, Technology and Research (ASTAR), Singapore, 138648 Singapore ; School of Biological Sciences, Nanyang Technological University, Singapore, 637551 Singapore ; Department of Biochemistry, Yong Loo Lin School of Medicine, NUS, Singapore, 117597 Singapore.

Genomic instability (GIN) is a hallmark of cancer cells that facilitates the acquisition of mutations conferring aggressive or drug-resistant phenotypes during cancer evolution. Chromosomal instability (CIN) is a form of GIN that involves frequent cytogenetic changes leading to changes in chromosome copy number (aneuploidy). While both CIN and aneuploidy are common characteristics of cancer cells, their roles in tumor initiation and progression are unclear. Read More

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http://dx.doi.org/10.1186/s13008-015-0009-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443636PMC
May 2015
16 Reads

Mutations in the PCNA-binding site of CDKN1C inhibit cell proliferation by impairing the entry into S phase.

Cell Div 2015 28;10. Epub 2015 Mar 28.

Department of Human Genetics, David Geffen School of Medicine at UCLA, University of California, Los Angeles, 695 Charles E. Young Drive, Los Angeles, CA 90095 USA ; Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, USA ; Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, USA.

CDKN1C (also known as P57 (kip2) ) is a cyclin-dependent kinase inhibitor that functions as a negative regulator of cell proliferation through G1 phase cell cycle arrest. Recently, our group described gain-of-function mutations in the PCNA-binding site of CDKN1C that result in an undergrowth syndrome called IMAGe Syndrome (Intrauterine Growth Restriction, Metaphyseal dysplasia, Adrenal hypoplasia, and Genital anomalies), with life-threatening consequences. Loss-of-function mutations in CDKN1C have been identified in 5-10% of individuals with Beckwith-Wiedemann syndrome (BWS), an overgrowth disorder with features that are the opposite of IMAGe syndrome. Read More

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http://dx.doi.org/10.1186/s13008-015-0008-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389716PMC
April 2015
9 Reads

Cyclin E2 is the predominant E-cyclin associated with NPAT in breast cancer cells.

Cell Div 2015 19;10. Epub 2015 Feb 19.

The Kinghorn Cancer Centre and Cancer Research Program, Garvan Institute of Medical Research, Sydney, NSW Australia ; St Vincent's Clinical School, Faculty of Medicine UNSW, Sydney, Australia.

Background: The cyclin E oncogene activates CDK2 to drive cells from G1 to S phase of the cell cycle to commence DNA replication. It coordinates essential cellular functions with the cell cycle including histone biogenesis, splicing, centrosome duplication and origin firing for DNA replication. The two E-cyclins, E1 and E2, are assumed to act interchangeably in these functions. Read More

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http://dx.doi.org/10.1186/s13008-015-0007-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349318PMC
March 2015
10 Reads

Polar electrostatic forces drive poleward chromosome motions.

Cell Div 2014 30;9. Epub 2014 Dec 30.

Department of Biology, Rutgers The State University of New Jersey, Camden, NJ 08102 USA.

Recent experiments revealing nanoscale electrostatic force generation at kinetochores for chromosome motions have prompted models for interactions between positively charged molecules in kinetochores and negative charge at and near the plus ends of microtubules. A clear picture of how kinetochores and centrosomes establish and maintain a dynamic coupling to microtubules for force generation during the complex motions of mitosis remains elusive. The molecular cell biology paradigm requires that specific molecules, or molecular geometries, for polar force generation be identified. Read More

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http://dx.doi.org/10.1186/s13008-014-0005-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339643PMC
February 2015
6 Reads

The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.

Cell Div 2014 31;9. Epub 2014 Oct 31.

Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences, 21 "Acad. George Bonchev" Str., 1113 Sofia, Bulgaria.

Background: The S-phase checkpoint aims to prevent cells from generation of extensive single-stranded DNA that predisposes to genome instability. The S. cerevisiae complex Tof1/Csm3/Mrc1 acts to restrain the replicative MCM helicase when DNA synthesis is prohibited. Read More

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http://celldiv.biomedcentral.com/articles/10.1186/1747-1028-
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http://dx.doi.org/10.1186/1747-1028-9-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221646PMC
November 2014
12 Reads

Differential expression of centrosome regulators in Her2+ breast cancer cells versus non-tumorigenic MCF10A cells.

Cell Div 2014 25;9. Epub 2014 Sep 25.

Department of Radiation Oncology, Winship Cancer Institute, Emory University School of Medicine, C3084, 1365C Clifton Road NE, Atlanta, GA 30322, USA.

Centrosome amplification (CA) amongst particular breast cancer subtypes (Her2+ subtype) is associated with genomic instability and aggressive tumor phenotypes. However, changes in signaling pathways associated with centrosome biology have not been fully explored in subtype specific models. Novel centrosome regulatory genes that are selectively altered in Her2+ breast cancer cells are of interest in discerning why CA is more prevalent in this subtype. Read More

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http://dx.doi.org/10.1186/1747-1028-9-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181616PMC
October 2014
19 Reads

Smurf2 E3 ubiquitin ligase modulates proliferation and invasiveness of breast cancer cells in a CNKSR2 dependent manner.

Cell Div 2014 31;9. Epub 2014 Aug 31.

Research Scholar, Cancer Research, Rajiv Gandhi Centre for Biotechnology, Trivandrum 695 014, Kerala, India.

Background: Smurf2 is a member of the HECT family of E3 ubiquitin ligases that play important roles in determining the competence of cells to respond to TGF- β/BMP signaling pathway. However, besides TGF-β/BMP pathway, Smurf2 regulates a repertoire of other signaling pathways ranging from planar cell polarity during embryonic development to cell proliferation, migration, differentiation and senescence. Expression of Smurf2 is found to be dysregulated in many cancers including breast cancer. Read More

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http://dx.doi.org/10.1186/1747-1028-9-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154384PMC
September 2014
17 Reads

The DNA repair component Metnase regulates Chk1 stability.

Cell Div 2014 9;9. Epub 2014 Jul 9.

Department of Medicine, University of Florida College of Medicine, 1600 Archer Rd SW, Gainesville, FL 32610, USA.

Chk1 both arrests replication forks and enhances repair of DNA damage by phosphorylation of downstream effectors. Metnase (also termed SETMAR) is a SET histone methylase and transposase nuclease protein that promotes both DNA double strand break (DSB) repair and re-start of stalled replication forks. We previously found that Chk1 phosphorylation of Metnase on S495 enhanced its DNA DSB repair activity but decreased its ability to re-start stalled replication forks. Read More

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http://dx.doi.org/10.1186/1747-1028-9-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094783PMC
July 2014
12 Reads

Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells.

Cell Div 2014 30;9. Epub 2014 Dec 30.

Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología (INCan)-Instituto de Investigaciones Biomédicas (IIB), Universidad Nacional Autónoma de México (UNAM), México, DF 14080 México ; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar S/N, Ciudad Universitaria, Coyoacán, México, DF 04510 México.

Background: Heterochromatin protein 1 (HP1) is important in the establishment, propagation, and maintenance of constitutive heterochromatin, especially at the pericentromeric region. HP1 might participate in recruiting and directing Mis12 to the centromere during interphase, and HP1 disruption or abrogation might lead to the loss of Mis12 incorporation into the kinetochore. Therefore, the centromere structure and kinetochore relaxation that are promoted in the absence of Mis12 could further induce chromosome instability (CIN) by reducing the capacity of the kinetochore to anchor microtubules. Read More

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http://dx.doi.org/10.1186/s13008-014-0006-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4343280PMC
March 2015
14 Reads

The COP9 signalosome subunit 6 (CSN6): a potential oncogene.

Cell Div 2013 Nov 28;8(1):14. Epub 2013 Nov 28.

Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, Jiangsu, P,R, China.

CSN6 is one subunit of the constitutive photomorphogenesis 9 (COP9) signalosome (CSN), which is an evolutionarily conserved multiprotein complex found in plants and animals and originally described as a repressor of light-dependent growth and transcription in Arabidopsis. CSN is homologous to the 19S lid subcomplex of the 26S proteasome, thus it has been postulated to be a regulator of the ubiquitin-proteasome pathway. In mammalian cells, it consists of eight subunits (CSN1-CSN8). Read More

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http://dx.doi.org/10.1186/1747-1028-8-14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175502PMC
November 2013
9 Reads

Defining a new vision for the retinoblastoma gene: report from the 3rd International Rb Meeting.

Cell Div 2013 Nov 21;8(1):13. Epub 2013 Nov 21.

Departments of Pediatrics and Genetics, Stanford University, Stanford, CA 94305, USA.

The retinoblastoma tumor suppressor (Rb) pathway is mutated in most, if not all human tumors. In the G0/G1 phase, Rb and its family members p107 and p130 inhibit the E2F family of transcription factors. In response to mitogenic signals, Cyclin-dependent kinases (CDKs) phosphorylate Rb family members, which results in the disruption of complexes between Rb and E2F family members and in the transcription of genes essential for S phase progression. Read More

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http://dx.doi.org/10.1186/1747-1028-8-13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866465PMC
November 2013
7 Reads

Cohesin loading factor Nipbl localizes to chromosome axes during mammalian meiotic prophase.

Cell Div 2013 Aug 22;8(1):12. Epub 2013 Aug 22.

IRDB, Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital, London W12 0NN, UK.

Background: Sister chromatid cohesion mediated by the cohesin complex is essential for accurate chromosome segregation during mitosis and meiosis. Loading of cohesin onto chromosomes is dependent on another protein complex called kollerin, containing Nipbl/Scc2 and Mau2/Scc4. Nipbl is an evolutionarily conserved large protein whose haploinsufficiency in humans causes a developmental disorder called Cornelia de Lange syndrome. Read More

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http://dx.doi.org/10.1186/1747-1028-8-12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765518PMC
August 2013
26 Reads

A pool of peptides extracted from wheat bud chromatin inhibits tumor cell growth by causing defective DNA synthesis.

Cell Div 2013 6;8:11. Epub 2013 Aug 6.

Department of Clinical and Experimental Medicine, University of Perugia, Faculty of Medicin, S.Andrea delle Fratte, 06132, Perugia, Italy.

Background: We previously reported that a pool of low molecular weight peptides can be extracted by alkali treatment of DNA preparations obtained from prokaryotic and eukaryotic cells after intensive deproteinization. This class of peptides, isolated from wheat bud chromatin, induces growth inhibition, DNA damage, G2 checkpoint activation and apoptosis in HeLa cells. In this work we studied their mechanism of action by investigating their ability to interfere with DNA synthesis. Read More

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http://dx.doi.org/10.1186/1747-1028-8-11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750333PMC
May 2014
7 Reads