3,081 results match your criteria Cell Calcium[Journal]


L-type calcium channel modulates mechanosensitivity of the cardiomyocyte cell line H9c2.

Cell Calcium 2019 Feb 22;79:68-74. Epub 2019 Feb 22.

Department of Physiology, Nagoya University School of Medicine, Nagoya, 466-8550, Japan; Mechanobiology Laboratory, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.

The application of mechanical stimuli to cells often induce increases in intracellular calcium, affecting the regulation of a variety of cell functions. Although the mechanism of mechanotransduction-induced calcium increases has not been fully resolved, the involvement of mechanosensitive ion channels in the plasma membrane and the endoplasmic reticulum has been reported. Here, we demonstrate that voltage-gated L-type calcium channels play a critical role in the mechanosensitive calcium response in H9c2 rat cardiomyocytes. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.008DOI Listing
February 2019
4 Reads

A novel STIM1-Orai1 gating interface essential for CRAC channel activation.

Cell Calcium 2019 Feb 23;79:57-67. Epub 2019 Feb 23.

Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria. Electronic address:

Calcium signalling through store-operated calcium (SOC) entry is of crucial importance for T-cell activation and the adaptive immune response. This entry occurs via the prototypic Ca release-activated Ca (CRAC) channel. STIM1, a key molecular component of this process, is located in the membrane of the endoplasmic reticulum (ER) and is initially activated upon Ca store depletion. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.009DOI Listing
February 2019

Familial Alzheimer's disease-linked presenilin mutants and intracellular Ca handling: A single-organelle, FRET-based analysis.

Cell Calcium 2019 Feb 23;79:44-56. Epub 2019 Feb 23.

Neuroscience Institute - Italian National Research Council (CNR), 35131 Padua, Italy; Department of Biomedical Sciences, University of Padua, Via U. Bassi 58/B, 35131 Padua, Italy.

An imbalance in Ca homeostasis represents an early event in the pathogenesis of Alzheimer's disease (AD). Presenilin-1 and -2 (PS1 and PS2) mutations, the major cause of familial AD (FAD), have been extensively associated with alterations in different Ca signaling pathways, in particular those handled by storage compartments. However, FAD-PSs effect on organelles Ca content is still debated and the mechanism of action of mutant proteins is unclear. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.005DOI Listing
February 2019

Numbers count: How STIM and Orai stoichiometry affect store-operated calcium entry.

Cell Calcium 2019 Feb 12;79:35-43. Epub 2019 Feb 12.

Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, 94305, United States. Electronic address:

Substantial progress has been made in the past several years in establishing the stoichiometries of STIM and Orai proteins and understanding their influence on store-operated calcium entry. Depletion of ER Ca triggers STIM1 to accumulate at ER-plasma membrane junctions where it binds and opens Ca release-activated Ca (CRAC) channels. STIM1 is a dimer, and release of Ca from its two luminal domains is reported to promote their association as well as drive formation of higher-order STIM1 oligomers. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.002DOI Listing
February 2019

A single point mutation in the TRPC3 lipid-recognition window generates supersensitivity to benzimidazole channel activators.

Cell Calcium 2019 Feb 16;79:27-34. Epub 2019 Feb 16.

Gottfried-Schatz Research Center (Biophysics), Medical University of Graz, Austria. Electronic address:

Mutation of a single residue within the recently identified lipid (diacylglycerol) recognition window of TRPC3 (G652A) was found to abolish channel activation via endogenous lipid mediators while retaining sensitivity to the non-lipid activator GSK1702934A (abb. GSK). The mechanism of this change in chemical sensing by TRPC3 was analysed by whole-cell and single channel electrophysiology as well as Ca imaging. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.007DOI Listing
February 2019

Getting close. Lysosome-ER contact sites tailor Ca signals.

Authors:
Sandip Patel

Cell Calcium 2019 Feb 8. Epub 2019 Feb 8.

Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom. Electronic address:

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http://dx.doi.org/10.1016/j.ceca.2019.02.003DOI Listing
February 2019
1 Read

Intracellular effect of β3-adrenoceptor agonist Carazolol on skeletal muscle, a direct interaction with SERCA.

Cell Calcium 2019 Feb 11;79:20-26. Epub 2019 Feb 11.

Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, México City, 04510, Mexico. Electronic address:

Carazolol (CZL) is a known agonist of β3 and antagonist of β1 and β2 adrenoceptors (AR), used in the animal production industry to improve meat quality by reducing animal stress and skeletal muscle (SM) proteolysis. Here we sought to better understand the direct effect CZL has on SM. We study CZL effect on calcium (Ca) regulation by enzymatic activity kinetics of the Ca-ATPase (SERCA), in isolated sarcoplasmic reticulum (SR) from SM and on the mechanical properties of isolated muscle. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.004DOI Listing
February 2019

Persistent Na influx drives L-type channel resting Ca entry in rat melanotrophs.

Cell Calcium 2019 Feb 6;79:11-19. Epub 2019 Feb 6.

Laboratory of Veterinary Physiology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. Electronic address:

Rat melanotrophs express several types of voltage-gated and ligand-gated calcium channels, although mechanisms involved in the maintenance of the resting intracellular Ca concentration ([Ca]) remain unknown. We analyzed mechanisms regulating resting [Ca] in dissociated rat melanotrophs by Ca-imaging and patch-clamp techniques. Treatment with antagonists of L-type, but not N- or P/Q-type voltage-gated Ca channels (VGCCs) as well as removal of extracellular Ca resulted in a rapid and reversible decrease in [Ca], indicating constitutive Ca influx through L-type VGCCs. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.02.001DOI Listing
February 2019
1 Read

ORAI channels in cellular remodeling of cardiorespiratory disease.

Cell Calcium 2019 Feb 8;79:1-10. Epub 2019 Feb 8.

Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, United States. Electronic address:

Cardiorespiratory disease, which includes systemic arterial hypertension, restenosis, atherosclerosis, pulmonary arterial hypertension, asthma, and chronic obstructive pulmonary disease (COPD) are highly prevalent and devastating diseases with limited therapeutic modalities. A common pathophysiological theme to these diseases is cellular remodeling, which is contributed by changes in expression and activation of ion channels critical for either excitability or growth. Calcium (Ca) signaling and specifically ORAI Ca channels have emerged as significant regulators of smooth muscle, endothelial, epithelial, platelet, and immune cell remodeling. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.01.005DOI Listing
February 2019
1 Read

A novel multi lines analysis tool of Ca dynamics reveals the nonuniformity of Ca propagation.

Cell Calcium 2019 Mar 4;78:76-80. Epub 2019 Jan 4.

Laboratory for Developmental Neurobiology, Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, Japan. Electronic address:

Extracellular stimuli evoke a robust increase in the concentration of intracellular Ca ([Ca]) throughout the cell to trigger various cellular responses, such as gene expression and apoptosis. This robust expansion of [Ca] is called Ca propagation. To date, it is thought that intracellular second messengers, such as inositol 1,4,5-trisphosphate (IP) and intracellular Ca, and clusters of IP receptors (IPRs) regulate Ca propagation. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.01.001DOI Listing

Methylglyoxal evokes acute Ca transients in distinct cell types and increases agonist-evoked Ca entry in endothelial cells via CRAC channels.

Cell Calcium 2019 Mar 9;78:66-75. Epub 2019 Jan 9.

Institute of Pharmacology, Heidelberg University, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany. Electronic address:

Methylglyoxal (MG) is a by-product of glucose metabolism and its accumulation has been linked to the development of diabetic complications such as retinopathy and nephropathy by affecting multiple signalling pathways. However, its influence on the intracellular Ca homeostasis and particularly Ca entry, which has been reported to be mediated via TRPA1 channels in DRG neurons, has not been studied in much detail in other cell types. In this study, we report the consequences of acute and long-term MG application on intracellular Ca levels in endothelial cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.01.002DOI Listing
March 2019
2 Reads

CRAC channel regulation of innate immune cells in health and disease.

Cell Calcium 2019 Mar 9;78:56-65. Epub 2019 Jan 9.

Department of Laboratory Medicine, University of California, San Francisco, CA, United States.

Calcium is a major intracellular signaling messenger in innate immune cells. Similar to other immune cell subsets, the majority of calcium entry into innate immune cells is induced by cell surface receptors that stimulate store-operated calcium entry through calcium-release activated calcium (CRAC) channels. Since the molecular description of the STIM family of calcium sensors and the ORAI family of CRAC channel proteins, the majority of studies support a dominant role for these proteins in calcium signaling in innate cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183018
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http://dx.doi.org/10.1016/j.ceca.2019.01.003DOI Listing
March 2019
8 Reads

CRAC channels in secretory epithelial cell function and disease.

Cell Calcium 2019 Mar 31;78:48-55. Epub 2018 Dec 31.

Epithelial Signaling and Transport Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, 20892, United States. Electronic address:

The receptor-evoked Ca signal in secretory epithelia mediate many cellular functions essential for cell survival and their most fundamental functions of secretory granules exocytosis and fluid and electrolyte secretion. Ca influx is a key component of the receptor-evoked Ca signal in secretory cell and is mediated by both TRPC and the STIM1-activated Orai1 channels that mediates the Ca release-activated current (CRAC) I. The core components of the receptor-evoked Ca signal are assembled at the ER/PM junctions where exchange of materials between the plasma membrane and internal organelles take place, including transfer of lipids and Ca. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377301PMC
March 2019
1 Read

Eliminating contraction during culture maintains global and local Ca dynamics in cultured rabbit pacemaker cells.

Cell Calcium 2019 Mar 18;78:35-47. Epub 2018 Dec 18.

Biomedical Engineering Faculty, Technion-IIT, Haifa, Israel. Electronic address:

Pacemaker cells residing in the sinoatrial node generate the regular heartbeat. Ca signaling controls the heartbeat rate-directly, through the effect on membrane molecules (NCX exchange, K channel), and indirectly, through activation of calmodulin-AC-cAMP-PKA signaling. Thus, the physiological role of signaling in pacemaker cells can only be assessed if the Ca dynamics are in the physiological range. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.008DOI Listing
March 2019
2 Reads

Discovery and characterization of a positive allosteric modulator of transient receptor potential canonical 6 (TRPC6) channels.

Cell Calcium 2019 Mar 21;78:26-34. Epub 2018 Dec 21.

Rudolf-Boehm-Institute of Pharmacology and Toxicology, Medical Faculty, Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany. Electronic address:

The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent inhibitors as well as channel-activating agents is of great interest. The screening of a chemical library, comprising about 17,000 small molecule compounds, revealed an agent, which induced increases in intracellular Ca concentrations ([Ca]) in a concentration-dependent manner (EC = 2. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.009DOI Listing
March 2019
1 Read

Spatiotemporal pattern of calcium activity in astrocytic network.

Authors:
Alexey Semyanov

Cell Calcium 2019 Mar 17;78:15-25. Epub 2018 Dec 17.

Department of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Institute of Neuroscience, University of Nizhny Novgorod, Nizhny Novgorod, Russia. Electronic address:

Ca influx through an astrocyte plasma membrane is mediated by ionotropic receptors and Ca channels according the electrochemical gradient. These conductances allow astrocytes to sense the levels of neuronal activity and environmental changes. Na/Ca exchanger (NCX) removes elevated Ca from the cell but can reverse and bring Ca in. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183022
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http://dx.doi.org/10.1016/j.ceca.2018.12.007DOI Listing
March 2019
13 Reads

Regulation of Ca signaling by acute hypoxia and acidosis in cardiomyocytes derived from human induced pluripotent stem cells.

Cell Calcium 2019 Mar 12;78:1-14. Epub 2018 Dec 12.

Cardiac Signaling Center of MUSC, USC and Clemson, Charleston, SC, USA; Department of Pharmacology,Georgetown University Medical Center, Washington, DC, USA. Electronic address:

Aims: The effects of acute (100 s) hypoxia and/or acidosis on Ca signaling parameters of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are explored here for the first time.

Methods And Results: 1) hiPSC-CMs express two cell populations: rapidly-inactivating I myocytes (τ<40 ms, in 4-5 day cultures) and slowly-inactivating I (τ  ≥ 40 ms, in 6-8 day cultures). 2) Hypoxia suppressed I by 10-20% in rapidly- and 40-55% in slowly-inactivating I cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183016
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http://dx.doi.org/10.1016/j.ceca.2018.12.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378877PMC
March 2019
10 Reads

Regulation of inositol 1,4,5-trisphosphate-induced Ca release from the endoplasmic reticulum by AMP-activated kinase modulators.

Cell Calcium 2019 Jan 11;77:68-76. Epub 2018 Dec 11.

Institute of Biology and Molecular Genetics (IBGM), Department of Biochemistry and Molecular Biology and Physiology, Faculty of Medicine, University of Valladolid and CSIC, Ramón y Cajal, 7, E-47005 Valladolid, Spain. Electronic address:

The 5' AMP-activated protein kinase (AMPK) is a nutrient-sensitive kinase that plays a key role in the control of cellular energy metabolism. We have explored here the relationship between AMPK and Ca signaling by looking at the effect of an AMPK activator (A769662) and an AMPK inhibitor (dorsomorphin) on histamine-induced Ca-release from the endoplasmic reticulum (ER) in HeLa cells. Our data show that incubation with A769662 (EC = 29 μM) inhibited histamine-induced Ca-release from the ER in intact cells, as well as inositol-1,4,5-trisphosphate (IP)-induced Ca release in permeabilized cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183016
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http://dx.doi.org/10.1016/j.ceca.2018.12.004DOI Listing
January 2019
7 Reads

Triskelion channels might bring Star Wars to the global problem of hypertension.

Authors:
David J Beech

Cell Calcium 2019 Jan 11;77:77-78. Epub 2018 Dec 11.

Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK. Electronic address:

In a compelling new report Zeng et al. (Science 2018) suggest that neuronal Piezo1 and Piezo2 channels sense blood pressure in major arteries above the heart [1]. The data challenge previous proposed baroreceptor mechanisms and add to prior knowledge of Piezo1 channels as sensors of blood flow and key players generally in cardiovascular biology. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183021
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http://dx.doi.org/10.1016/j.ceca.2018.12.005DOI Listing
January 2019
9 Reads

EGR-mediated control of STIM expression and function.

Cell Calcium 2019 Jan 6;77:58-67. Epub 2018 Dec 6.

Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, 19140, USA; Department of Medical Genetics & Molecular Biochemistry, Temple University School of Medicine, Philadelphia, PA, 19140, USA. Electronic address:

Ca is a ubiquitous, dynamic and pluripotent second messenger with highly context-dependent roles in complex cellular processes such as differentiation, proliferation, and cell death. These Ca signals are generated by Ca-permeable channels located on the plasma membrane (PM) and endoplasmic reticulum (ER) and shaped by PM- and ER-localized pumps and transporters. Differences in the expression of these Ca homeostasis proteins contribute to cell and context-dependent differences in the spatiotemporal organization of Ca signals and, ultimately, cell fate. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.003DOI Listing
January 2019
10 Reads

Calcium extrusion mechanisms in dendrites of mouse hippocampal CA1 inhibitory interneurons.

Cell Calcium 2019 Jan 4;77:49-57. Epub 2018 Dec 4.

Department of Biochemistry, Microbiology and Bio-Informatics, Faculty of Science and Engineering, Laval University, Québec, PQ, Canada; Neuroscience Axis, CHU de Québec Research Center, Laval University, Québec, PQ, Canada. Electronic address:

Local circuit GABAergic inhibitory interneurons control the integration and transfer of information in many brain regions. Several different forms of plasticity reported at interneuron excitatory synapses are triggered by cell- and synapse-specific postsynaptic calcium (Ca) mechanisms. To support this function, the spatiotemporal dynamics of dendritic Ca elevations must be tightly regulated. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.002DOI Listing
January 2019
1 Read

Store-operated calcium entry in thrombosis and thrombo-inflammation.

Cell Calcium 2019 Jan 23;77:39-48. Epub 2018 Nov 23.

Institute of Experimental Biomedicine, University Hospital and Rudolf Virchow Centre, University of Würzburg, Würzburg, Germany. Electronic address:

Cytosolic free calcium (Ca) is a second messenger regulating a wide variety of functions in blood cells, including adhesion, activation, proliferation and migration. Store-operated Ca entry (SOCE), triggered by depletion of Ca from the endoplasmic reticulum, provides a main mechanism of regulated Ca influx in blood cells. SOCE is mediated and regulated by isoforms of the ion channel proteins ORAI and TRP, and the transmembrane Ca sensors stromal interaction molecules (STIMs), respectively. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.005DOI Listing
January 2019
1 Read

STIM1 activation of Orai1.

Cell Calcium 2019 Jan 30;77:29-38. Epub 2018 Nov 30.

Institute of Biophysics, Johannes Kepler University Linz, A-4020, Linz, Austria. Electronic address:

A primary calcium (Ca) entry pathway into non-excitable cells is through the store-operated Ca release activated Ca (CRAC) channel. Ca entry into cells is responsible for the initiation of diverse signalling cascades that affect essential cellular processes like gene regulation, cell growth and death, secretion and gene transcription. Upon depletion of intracellular Ca stores within the endoplasmic reticulum (ER), the CRAC channel opens to refill depleted stores. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.009DOI Listing
January 2019
3 Reads

Does stromal interaction molecule-1 have five senses?

Cell Calcium 2019 Jan 3;77:79-80. Epub 2018 Dec 3.

Department of Medical Biophysics, University of Toronto, Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2M9, Canada. Electronic address:

A single calcium (Ca) binding site within the canonical EF-hand loop was thought to govern the stromal interaction molecule-1 (STIM1) structural changes that lead to activation of Orai1 Ca channels. Recent work by Gudlur et al., published in Nat Commun [9(1):4536], suggests that the STIM1 endoplasmic reticulum (ER) luminal domain has ∼5 additional Ca binding sites, which underlie a surprising new proposal for Ca sensing. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.001DOI Listing
January 2019
1 Read

SOCE and STIM1 signaling in the heart: Timing and location matter.

Cell Calcium 2019 Jan 27;77:20-28. Epub 2018 Nov 27.

Department of Medicine, Duke University School of Medicine, Durham, NC, United States.

Store operated Ca entry (SOCE) is an ancient and ubiquitous Ca signaling pathway discovered decades ago, but the function of SOCE in human physiology is only now being revealed. The relevance of this pathway to striated muscle was solidified with the description of skeletal myopathies that result from mutations in STIM1 and Orai1, the two SOCE components. Here, we consider the evidence for STIM1 and SOCE in cardiac muscle and the sinoatrial node. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.008DOI Listing
January 2019
3 Reads

Downregulation of TRPC6 expression is a critical molecular event during FK506 treatment for overactive bladder.

Cell Calcium 2019 Jan 17;77:8-19. Epub 2018 Nov 17.

Department of Urology, the Fourth Affiliated Hospital of China Medical University, No.4, Chong-shan East Road, Shenyang 110032, Liaoning Province, PR China. Electronic address:

Purpose: It has been suggested that FK506 could improve some symptoms of OAB in both clinical settings and animal models; however, its mechanism of action is not well-understood. Here, we investigated the effect of FK506 on TRPC6 in bladder smooth muscle, and explored the possible involvement of TRPC6 in OAB.

Methods: FK506 was injected intraperitoneally into rats in which OAB was induced via BOO, and urodynamic indices were recorded. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183008
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http://dx.doi.org/10.1016/j.ceca.2018.11.007DOI Listing
January 2019
12 Reads

Effects of his-tags on physical properties of parvalbumins.

Cell Calcium 2019 Jan 16;77:1-7. Epub 2018 Nov 16.

Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia. Electronic address:

A comparative study of His-tagged and non-tagged rat β-parvalbumin (rWT β-PA), calcium binding protein with the EF-hand calcium binding domains, has been carried out. The attachment of His-tag increases α-helical content and decreases β-sheets and β-turns content of the metal free form (apo-state) of β-PA. In contrast to this, the attachment of His-tag decreases α-helical content by more than 10% and increases contents of β-sheets and β-turns of the Ca-loaded state. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.006DOI Listing
January 2019
1 Read
3.513 Impact Factor

TRPM7 reflected in Cryo-EMirror.

Cell Calcium 2018 Dec 15;76:129-131. Epub 2018 Nov 15.

Walther Straub Institute of Pharmacology and Toxicology, LMU Munich, Germany; German Center for Lung Research, Munich, Germany; German Centre for Cardiovascular Research, Munich Heart Alliance, Munich, Germany. Electronic address:

TRPM7 is an atypical type of ion channel because its pore-forming moiety is covalently linked to a protein kinase domain. The channel-kinase TRPM7 controls a wide range of biological processes such as mineral homeostasis, immune responses, cell motility, proliferation and differentiation. Earlier this year, Duan J & co-workers [1] published three TRPM7 structures resolved by cryo-electron microscopy (cryo-EM). Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.004DOI Listing
December 2018
8 Reads

Protective effect of leptin-mediated caveolin-1 expression on neurons after spinal cord injury.

Cell Calcium 2018 Dec 13;76:122-128. Epub 2018 Nov 13.

Department of Orthopaedics, First Affiliated Hospital, China Medical University, Shenyang, People's Republic of China. Electronic address:

Spinal cord injury (SCI) causes long-term disability and has no effective clinical treatment. After SCI, extracellular adenosine triphosphate (ATP) leads to an influx of extracellular Ca, and this Ca overload causes neuronal toxicosis and apoptosis. The biological functions of leptin have been widely investigated in the central nervous system. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.003DOI Listing
December 2018
13 Reads
3.513 Impact Factor

Endoplasmic reticulum calcium dictates the distribution of intracellular unesterified cholesterol.

Cell Calcium 2018 Dec 10;76:116-121. Epub 2018 Nov 10.

Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2S7, Canada. Electronic address:

Endoplasmic reticulum (ER) luminal Ca influences many functions of this organelle, notably the synthesis and quality control of proteins and lipids. Cholesterol is an essential component of biological membranes and a precursor for many biologically important signaling molecules. The sterol regulatory element-binding proteins (SREBPs) are key regulators of lipid metabolism. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.002DOI Listing
December 2018
16 Reads

TRPM2 activation: Paradigm shifted?

Cell Calcium 2018 Dec 3;76:132-134. Epub 2018 Nov 3.

The Calcium Signaling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany. Electronic address:

Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it really a paradigm shift or just reflects evolution of the channel?. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183019
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http://dx.doi.org/10.1016/j.ceca.2018.11.001DOI Listing
December 2018
9 Reads

Role of STIM1/ORAI1-mediated store-operated Ca entry in skeletal muscle physiology and disease.

Cell Calcium 2018 Dec 30;76:101-115. Epub 2018 Oct 30.

Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY, 14642, United States. Electronic address:

Store-operated Ca entry (SOCE) is a Ca entry mechanism activated by depletion of intracellular Ca stores. In skeletal muscle, SOCE is mediated by an interaction between stromal-interacting molecule-1 (STIM1), the Ca sensor of the sarcoplasmic reticulum, and ORAI1, the Ca-release-activated-Ca (CRAC) channel located in the transverse tubule membrane. This review focuses on the molecular mechanisms and physiological role of SOCE in skeletal muscle, as well as how alterations in STIM1/ORAI1-mediated SOCE contribute to muscle disease. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183013
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http://dx.doi.org/10.1016/j.ceca.2018.10.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290926PMC
December 2018
10 Reads

Hold the door: hPMCA1/neuroplastin interactions regulate Ca-binding site accessibility.

Cell Calcium 2018 Dec 1;76:135-136. Epub 2018 Nov 1.

Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, 19140, United States; Department of Medical Genetics & Molecular Biochemistry, Temple University School of Medicine, Philadelphia, PA, 19140, United States. Electronic address:

In a September 2018 paper published in Nature Communications, Gong et al. identified the domains through which human PMCA1 and neuroplastin (NPTN) interact. Upon binding, hPMCA1 TM domains separate T110 in TM1 and A370 in TM3 to reveal the Ca-binding site. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.10.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309627PMC
December 2018
1 Read

STIM1 R304W causes muscle degeneration and impaired platelet activation in mice.

Cell Calcium 2018 Dec 5;76:87-100. Epub 2018 Oct 5.

Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway. Electronic address:

STIM1 and ORAI1 regulate store-operated Ca entry (SOCE) in most cell types, and mutations in these proteins have deleterious and diverse effects. We established a mouse line expressing the STIM1 R304 W gain-of-function mutation causing Stormorken syndrome to explore effects on organ and cell physiology. While STIM1 R304 W was lethal in the homozygous state, surviving mice presented with reduced growth, skeletal muscle degeneration, and reduced exercise endurance. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183016
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http://dx.doi.org/10.1016/j.ceca.2018.10.001DOI Listing
December 2018
14 Reads

The multifaceted STAT3: How a transcription factor regulates Ca signaling via a degradative pathway.

Authors:
Jan B Parys

Cell Calcium 2018 Dec 12;76:137-139. Epub 2018 Oct 12.

KU Leuven, Laboratory for Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine & Leuven Kanker Instituut, Campus Gasthuisberg O/N-1 B-802, Herestraat 49, BE-3000 Leuven, Belgium. Electronic address:

STAT3 is a pleiotropic prosurvival transcription factor with functions in nucleus and mitochondria. Avalle et al. (Cell Death Diff. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183018
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http://dx.doi.org/10.1016/j.ceca.2018.10.002DOI Listing
December 2018
5 Reads

Transcriptomic changes in C2C12 myotubes triggered by electrical stimulation: Role of Ca-mediated and Ca-independent signaling and elevated [Na]/[K] ratio.

Cell Calcium 2018 Dec 30;76:72-86. Epub 2018 Sep 30.

M. V. Lomonosov Moscow State University, Moscow, Russia; National Research Tomsk State University, Tomsk, Russia; Siberian Medical State University, Tomsk, Russia. Electronic address:

Elevation of Ca and AMP-activated protein kinase (AMPK) are considered as major signals triggering transcriptomic changes in exercising skeletal muscle. Electrical pulse stimulation (EPS) of cultured myotubes is widely employed as an in vitro model of muscle contraction. This study examines the impact of Ca-mediated and Ca-independent signaling in transcriptomic changes in EPS-treated C2C12 myotubes. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.007DOI Listing
December 2018
3 Reads

Lysosomal exocytosis of ATP is coupled to P2Y receptor in marginal cells in the stria vascular in neonatal rats.

Cell Calcium 2018 Dec 21;76:62-71. Epub 2018 Sep 21.

Department of Otorhinolaryngology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address:

Adenosine triphosphate (ATP) is stored as lysosomal vesicles in marginal cells of the stria vascular in neonatal rats, but the mechanisms of ATP release are unclear. Primary cultures of marginal cells from 1-day-old Sprague-Dawley rats were established. P2Y receptor and inositol 1,4,5-trisphosphate (IP3) receptor were immunolabelled in marginal cells of the stria vascular. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.006DOI Listing
December 2018
3 Reads
3.513 Impact Factor

Nonlinear relationship between ER Ca depletion versus induction of the unfolded protein response, autophagy inhibition, and cell death.

Cell Calcium 2018 Dec 17;76:48-61. Epub 2018 Sep 17.

Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership for Molecular Medicine, University of Oslo, Norway. Electronic address:

Endoplasmic reticulum (ER) Ca depletion activates the unfolded protein response (UPR), inhibits bulk autophagy and eventually induces cell death in mammalian cells. However, the extent and duration of ER Ca depletion required is unknown. We instigated a detailed study in two different cell lines, using sarco/endoplasmic reticulum Ca-ATPase (SERCA) inhibitors to gradually reduce ER Ca levels in a controlled manner. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.005DOI Listing
December 2018
9 Reads

Spontaneous Ca transients in rat pulmonary vein cardiomyocytes are increased in frequency and become more synchronous following electrical stimulation.

Cell Calcium 2018 Dec 16;76:36-47. Epub 2018 Sep 16.

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK. Electronic address:

The pulmonary veins have an external sleeve of cardiomyocytes that are a widely recognised source of ectopic electrical activity that can lead to atrial fibrillation. Although the mechanisms behind this activity are currently unknown, changes in intracellular calcium (Ca) signalling are purported to play a role. Therefore, the intracellular Ca concentration was monitored in the pulmonary vein using fluo-4 and epifluorescence microscopy. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.001DOI Listing
December 2018
21 Reads

Data-driven modeling of mitochondrial dysfunction in Alzheimer's disease.

Cell Calcium 2018 Dec 12;76:23-35. Epub 2018 Sep 12.

Department of Physics, University of South Florida, Tampa, FL 33620, USA. Electronic address:

Intracellular accumulation of oligomeric forms of β amyloid (Aβ) are now believed to play a key role in the earliest phase of Alzheimer's disease (AD) as their rise correlates well with the early symptoms of the disease. Extensive evidence points to impaired neuronal Ca homeostasis as a direct consequence of the intracellular Aβ oligomers. However, little is known about the downstream effects of the resulting Ca rise on the many intracellular Ca-dependent pathways. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289702PMC
December 2018
13 Reads

Key residues controlling bidirectional ion movements in Na/Ca exchanger.

Cell Calcium 2018 Dec 15;76:10-22. Epub 2018 Sep 15.

Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, 69978, Israel. Electronic address:

Prokaryotic and eukaryotic Na/Ca exchangers (NCX) control Ca homeostasis. NCX orthologs exhibit up to 10-fold differences in their turnover rates (k), whereas the ratios between the cytosolic (cyt) and extracellular (ext) K values (K = K/K) are highly asymmetric and alike (K ≤ 0.1) among NCXs. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.004DOI Listing
December 2018
1 Read

Gain-of-function mutations in STIM1 and ORAI1 causing tubular aggregate myopathy and Stormorken syndrome.

Cell Calcium 2018 Dec 3;76:1-9. Epub 2018 Sep 3.

Departement of Translational Medicine and Neurogenetics, IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), Inserm U1258, CNRS UMR7104, Université de Strasbourg, Illkirch, France.

Calcium (Ca) is a key regulator for a large number of cellular functions in all kinds of cells, and small disturbances of Ca homeostasis can severely compromise normal physiology in various tissues and organs. A major mechanism controlling Ca homeostasis is store-operated Ca entry (SOCE), which relies on the concerted action of the reticular Ca sensor STIM1 and the plasma membrane Ca channel ORAI1. Gain-of-function mutations in the respective genes induce excessive Ca entry, and cause tubular aggregate myopathy (TAM) and Stormorken syndrome. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.008DOI Listing
December 2018
4 Reads

Regulation of proto-oncogene Orai3 by miR18a/b and miR34a.

Cell Calcium 2018 11 3;75:101-111. Epub 2018 Sep 3.

CSIR- Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110025, India. Electronic address:

Store Operated Ca Entry (SOCE) mediated by Orai channels is a ubiquitous Ca influx pathway that regulates several cellular functions. We have earlier reported that Orai3, the mammalian specific Orai1 homolog, plays a critical role in breast cancer progression. More recently, Orai3 was demonstrated to regulate prostate and lung tumorigenesis. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.006DOI Listing
November 2018
1 Read
3.513 Impact Factor

Calmodulin-binding proteins: A journey of 40 years.

Cell Calcium 2018 11 5;75:89-100. Epub 2018 Sep 5.

Department of Pathology & Laboratory Medicine, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon S7N 5E5, Canada.

The proteins which bind to calmodulin in a Ca-dependent and reversible manner are known as calmodulin-binding proteins. These proteins are involved in a multitude of processes in which Ca and calmodulin play crucial roles. Our group elucidated the mechanism and importance of these proteins in normal and diseased conditions. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.002DOI Listing
November 2018
2 Reads

CRAC channel-based optogenetics.

Cell Calcium 2018 11 3;75:79-88. Epub 2018 Sep 3.

Center for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, Houston, TX 77030, USA; Department of Medical Physiology, College of Medicine, Texas A&M University, Temple, TX 76504, USA. Electronic address:

Store-operated Ca² entry (SOCE) constitutes a major Ca influx pathway in mammals to regulate a myriad of physiological processes, including muscle contraction, synaptic transmission, gene expression, and metabolism. In non-excitable cells, the Ca² release-activated Ca² (CRAC) channel, composed of ORAI and stromal interaction molecules (STIM), constitutes a prototypical example of SOCE to mediate Ca entry at specialized membrane contact sites (MCSs) between the endoplasmic reticulum (ER) and the plasma membrane (PM). The key steps of SOCE activation include the oligomerization of the luminal domain of the ER-resident Ca sensor STIM1 upon Ca² store depletion, subsequent signal propagation toward the cytoplasmic domain to trigger a conformational switch and overcome the intramolecular autoinhibition, and ultimate exposure of the minimal ORAI-activating domain to directly engage and gate ORAI channels in the plasma membrane. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183012
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http://dx.doi.org/10.1016/j.ceca.2018.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176914PMC
November 2018
19 Reads

Comprehensive analysis of the roles of 'black' and 'gray' clusters in structure and function of rat β-parvalbumin.

Cell Calcium 2018 11 27;75:64-78. Epub 2018 Aug 27.

Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia; Department of Biomedical Engineering, Pushchino State Institute of Natural Sciences, Pushchino, Moscow Region, 142290, Russia.

Recently we found two highly conserved structural motifs in the proteins of the EF-hand calcium binding protein family. These motifs provide a supporting scaffold for the Ca binding loops and contribute to the hydrophobic core of the EF-hand domain. Each structural motif forms a cluster of three amino acids called cluster I ('black' cluster) and cluster II ('grey' cluster). Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.005DOI Listing
November 2018
5 Reads
3.513 Impact Factor

Residues important for Ca ion transport in the neuronal K-dependent Na-Ca exchanger (NCKX2).

Cell Calcium 2018 09 30;74:187-197. Epub 2018 Jun 30.

Department of Physiology and Pharmacology, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:

K-dependent Na-Ca exchangers (NCKXs) belong to Ca/cation antiporter gene superfamily. NCKX proteins play an important role in Ca homeostasis and are bi-directional plasma membrane Ca-transporters which utilize the inward Na and outward K gradients to move Ca ions into and out of the cytosol (4Na:1Ca + 1 K). In this study, we examined residues in the two regions with the highest degree of homology between the different NCKX isoforms (α-1 and α-2 repeats) to determine which residues are important for Ca coordination. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.06.002DOI Listing
September 2018
16 Reads

How cellular Zn signaling drives physiological functions.

Cell Calcium 2018 11 18;75:53-63. Epub 2018 Aug 18.

Department of Physiology and Cell Biology and The Zlotowski Center for Neuroscience, Faculty of Health Sciences, POB 653, Ben-Gurion Ave., Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel. Electronic address:

Zinc is an essential micronutrient affecting many aspects of human health. Cellular Zn homeostasis is critical for cell function and survival. Zn, acting as a first or second messenger, triggers signaling pathways that mediate the physiological roles of Zn. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.004DOI Listing
November 2018
2 Reads

A screening campaign in sea urchin egg homogenate as a platform for discovering modulators of NAADP-dependent Ca signaling in human cells.

Cell Calcium 2018 11 16;75:42-52. Epub 2018 Aug 16.

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee WI 53226, USA. Electronic address:

The Ca mobilizing second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) regulates intracellular trafficking events, including translocation of certain enveloped viruses through the endolysosomal system. Targeting NAADP-evoked Ca signaling may therefore be an effective strategy for discovering novel antivirals as well as therapeutics for other disorders. To aid discovery of novel scaffolds that modulate NAADP-evoked Ca signaling in human cells, we have investigated the potential of using the sea urchin egg homogenate system for a screening campaign. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286156PMC
November 2018
2 Reads

NAADP-dependent Ca signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system.

Cell Calcium 2018 11 9;75:30-41. Epub 2018 Aug 9.

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee WI 53226, USA. Electronic address:

Middle East Respiratory Syndrome coronavirus (MERS-CoV) infections are associated with a significant mortality rate, and existing drugs show poor efficacy. Identifying novel targets/pathways required for MERS infectivity is therefore important for developing novel therapeutics. As an enveloped virus, translocation through the endolysosomal system provides one pathway for cellular entry of MERS-CoV. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251489PMC
November 2018
21 Reads
3.510 Impact Factor