3,064 results match your criteria Cell Calcium[Journal]


Regulation of inositol 1,4,5-trisphosphate-induced Ca release from the endoplasmic reticulum by AMP-activated kinase modulators.

Cell Calcium 2018 Dec 11;77:68-76. Epub 2018 Dec 11.

Institute of Biology and Molecular Genetics (IBGM), Department of Biochemistry and Molecular Biology and Physiology, Faculty of Medicine, University of Valladolid and CSIC, Ramón y Cajal, 7, E-47005 Valladolid, Spain. Electronic address:

The 5' AMP-activated protein kinase (AMPK) is a nutrient-sensitive kinase that plays a key role in the control of cellular energy metabolism. We have explored here the relationship between AMPK and Ca signaling by looking at the effect of an AMPK activator (A769662) and an AMPK inhibitor (dorsomorphin) on histamine-induced Ca-release from the endoplasmic reticulum (ER) in HeLa cells. Our data show that incubation with A769662 (EC = 29 μM) inhibited histamine-induced Ca-release from the ER in intact cells, as well as inositol-1,4,5-trisphosphate (IP)-induced Ca release in permeabilized cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.004DOI Listing
December 2018

Triskelion channels might bring Star Wars to the global problem of hypertension.

Authors:
David J Beech

Cell Calcium 2018 Dec 11. Epub 2018 Dec 11.

Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK. Electronic address:

In a compelling new report Zeng et al. (Science 2018) suggest that neuronal Piezo1 and Piezo2 channels sense blood pressure in major arteries above the heart [1]. The data challenge previous proposed baroreceptor mechanisms and add to prior knowledge of Piezo1 channels as sensors of blood flow and key players generally in cardiovascular biology. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183021
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http://dx.doi.org/10.1016/j.ceca.2018.12.005DOI Listing
December 2018
1 Read

EGR-mediated control of STIM expression and function.

Cell Calcium 2018 Dec 6;77:58-67. Epub 2018 Dec 6.

Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, 19140, USA; Department of Medical Genetics & Molecular Biochemistry, Temple University School of Medicine, Philadelphia, PA, 19140, USA. Electronic address:

Ca is a ubiquitous, dynamic and pluripotent second messenger with highly context-dependent roles in complex cellular processes such as differentiation, proliferation, and cell death. These Ca signals are generated by Ca-permeable channels located on the plasma membrane (PM) and endoplasmic reticulum (ER) and shaped by PM- and ER-localized pumps and transporters. Differences in the expression of these Ca homeostasis proteins contribute to cell and context-dependent differences in the spatiotemporal organization of Ca signals and, ultimately, cell fate. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.003DOI Listing
December 2018
1 Read

Calcium extrusion mechanisms in dendrites of mouse hippocampal CA1 inhibitory interneurons.

Cell Calcium 2018 Dec 4;77:49-57. Epub 2018 Dec 4.

Department of Biochemistry, Microbiology and Bio-Informatics, Faculty of Science and Engineering, Laval University, Québec, PQ, Canada; Neuroscience Axis, CHU de Québec Research Center, Laval University, Québec, PQ, Canada. Electronic address:

Local circuit GABAergic inhibitory interneurons control the integration and transfer of information in many brain regions. Several different forms of plasticity reported at interneuron excitatory synapses are triggered by cell- and synapse-specific postsynaptic calcium (Ca) mechanisms. To support this function, the spatiotemporal dynamics of dendritic Ca elevations must be tightly regulated. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.002DOI Listing
December 2018

Store-operated calcium entry in thrombosis and thrombo-inflammation.

Cell Calcium 2018 Nov 23;77:39-48. Epub 2018 Nov 23.

Institute of Experimental Biomedicine, University Hospital and Rudolf Virchow Centre, University of Würzburg, Würzburg, Germany. Electronic address:

Cytosolic free calcium (Ca) is a second messenger regulating a wide variety of functions in blood cells, including adhesion, activation, proliferation and migration. Store-operated Ca entry (SOCE), triggered by depletion of Ca from the endoplasmic reticulum, provides a main mechanism of regulated Ca influx in blood cells. SOCE is mediated and regulated by isoforms of the ion channel proteins ORAI and TRP, and the transmembrane Ca sensors stromal interaction molecules (STIMs), respectively. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.005DOI Listing
November 2018

STIM1 activation of Orai1.

Cell Calcium 2018 Nov 30;77:29-38. Epub 2018 Nov 30.

Institute of Biophysics, Johannes Kepler University Linz, A-4020, Linz, Austria. Electronic address:

A primary calcium (Ca) entry pathway into non-excitable cells is through the store-operated Ca release activated Ca (CRAC) channel. Ca entry into cells is responsible for the initiation of diverse signalling cascades that affect essential cellular processes like gene regulation, cell growth and death, secretion and gene transcription. Upon depletion of intracellular Ca stores within the endoplasmic reticulum (ER), the CRAC channel opens to refill depleted stores. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.009DOI Listing
November 2018

Does stromal interaction molecule-1 have five senses?

Cell Calcium 2018 Dec 3. Epub 2018 Dec 3.

Department of Medical Biophysics, University of Toronto, Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2M9, Canada. Electronic address:

A single calcium (Ca) binding site within the canonical EF-hand loop was thought to govern the stromal interaction molecule-1 (STIM1) structural changes that lead to activation of Orai1 Ca channels. Recent work by Gudlur et al., published in Nat Commun [9(1):4536], suggests that the STIM1 endoplasmic reticulum (ER) luminal domain has ∼5 additional Ca binding sites, which underlie a surprising new proposal for Ca sensing. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.001DOI Listing
December 2018

SOCE and STIM1 signaling in the heart: Timing and location matter.

Cell Calcium 2018 Nov 27;77:20-28. Epub 2018 Nov 27.

Department of Medicine, Duke University School of Medicine, Durham, NC, United States.

Store operated Ca entry (SOCE) is an ancient and ubiquitous Ca signaling pathway discovered decades ago, but the function of SOCE in human physiology is only now being revealed. The relevance of this pathway to striated muscle was solidified with the description of skeletal myopathies that result from mutations in STIM1 and Orai1, the two SOCE components. Here, we consider the evidence for STIM1 and SOCE in cardiac muscle and the sinoatrial node. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.008DOI Listing
November 2018
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Downregulation of TRPC6 expression is a critical molecular event during FK506 treatment for overactive bladder.

Cell Calcium 2018 Nov 17;77:8-19. Epub 2018 Nov 17.

Department of Urology, the Fourth Affiliated Hospital of China Medical University, No.4, Chong-shan East Road, Shenyang 110032, Liaoning Province, PR China. Electronic address:

Purpose: It has been suggested that FK506 could improve some symptoms of OAB in both clinical settings and animal models; however, its mechanism of action is not well-understood. Here, we investigated the effect of FK506 on TRPC6 in bladder smooth muscle, and explored the possible involvement of TRPC6 in OAB.

Methods: FK506 was injected intraperitoneally into rats in which OAB was induced via BOO, and urodynamic indices were recorded. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183008
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http://dx.doi.org/10.1016/j.ceca.2018.11.007DOI Listing
November 2018
5 Reads

Effects of his-tags on physical properties of parvalbumins.

Cell Calcium 2018 Nov 16;77:1-7. Epub 2018 Nov 16.

Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia. Electronic address:

A comparative study of His-tagged and non-tagged rat β-parvalbumin (rWT β-PA), calcium binding protein with the EF-hand calcium binding domains, has been carried out. The attachment of His-tag increases α-helical content and decreases β-sheets and β-turns content of the metal free form (apo-state) of β-PA. In contrast to this, the attachment of His-tag decreases α-helical content by more than 10% and increases contents of β-sheets and β-turns of the Ca-loaded state. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.006DOI Listing
November 2018
3.513 Impact Factor

TRPM7 reflected in Cryo-EMirror.

Cell Calcium 2018 Dec 15;76:129-131. Epub 2018 Nov 15.

Walther Straub Institute of Pharmacology and Toxicology, LMU Munich, Germany; German Center for Lung Research, Munich, Germany; German Centre for Cardiovascular Research, Munich Heart Alliance, Munich, Germany. Electronic address:

TRPM7 is an atypical type of ion channel because its pore-forming moiety is covalently linked to a protein kinase domain. The channel-kinase TRPM7 controls a wide range of biological processes such as mineral homeostasis, immune responses, cell motility, proliferation and differentiation. Earlier this year, Duan J & co-workers [1] published three TRPM7 structures resolved by cryo-electron microscopy (cryo-EM). Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.004DOI Listing
December 2018
1 Read

Protective effect of leptin-mediated caveolin-1 expression on neurons after spinal cord injury.

Cell Calcium 2018 Dec 13;76:122-128. Epub 2018 Nov 13.

Department of Orthopaedics, First Affiliated Hospital, China Medical University, Shenyang, People's Republic of China. Electronic address:

Spinal cord injury (SCI) causes long-term disability and has no effective clinical treatment. After SCI, extracellular adenosine triphosphate (ATP) leads to an influx of extracellular Ca, and this Ca overload causes neuronal toxicosis and apoptosis. The biological functions of leptin have been widely investigated in the central nervous system. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.003DOI Listing
December 2018
4 Reads
3.510 Impact Factor

Endoplasmic reticulum calcium dictates the distribution of intracellular unesterified cholesterol.

Cell Calcium 2018 Dec 10;76:116-121. Epub 2018 Nov 10.

Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2S7, Canada. Electronic address:

Endoplasmic reticulum (ER) luminal Ca influences many functions of this organelle, notably the synthesis and quality control of proteins and lipids. Cholesterol is an essential component of biological membranes and a precursor for many biologically important signaling molecules. The sterol regulatory element-binding proteins (SREBPs) are key regulators of lipid metabolism. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.11.002DOI Listing
December 2018
5 Reads

TRPM2 activation: Paradigm shifted?

Cell Calcium 2018 Dec 3;76:132-134. Epub 2018 Nov 3.

The Calcium Signaling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany. Electronic address:

Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it really a paradigm shift or just reflects evolution of the channel?. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183019
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http://dx.doi.org/10.1016/j.ceca.2018.11.001DOI Listing
December 2018
2 Reads

Role of STIM1/ORAI1-mediated store-operated Ca entry in skeletal muscle physiology and disease.

Cell Calcium 2018 Dec 30;76:101-115. Epub 2018 Oct 30.

Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY, 14642, United States. Electronic address:

Store-operated Ca entry (SOCE) is a Ca entry mechanism activated by depletion of intracellular Ca stores. In skeletal muscle, SOCE is mediated by an interaction between stromal-interacting molecule-1 (STIM1), the Ca sensor of the sarcoplasmic reticulum, and ORAI1, the Ca-release-activated-Ca (CRAC) channel located in the transverse tubule membrane. This review focuses on the molecular mechanisms and physiological role of SOCE in skeletal muscle, as well as how alterations in STIM1/ORAI1-mediated SOCE contribute to muscle disease. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183013
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http://dx.doi.org/10.1016/j.ceca.2018.10.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290926PMC
December 2018
3 Reads

Hold the door: hPMCA1/neuroplastin interactions regulate Ca-binding site accessibility.

Cell Calcium 2018 Dec 1;76:135-136. Epub 2018 Nov 1.

Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, 19140, United States; Department of Medical Genetics & Molecular Biochemistry, Temple University School of Medicine, Philadelphia, PA, 19140, United States. Electronic address:

In a September 2018 paper published in Nature Communications, Gong et al. identified the domains through which human PMCA1 and neuroplastin (NPTN) interact. Upon binding, hPMCA1 TM domains separate T110 in TM1 and A370 in TM3 to reveal the Ca-binding site. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.10.003DOI Listing
December 2018

STIM1 R304W causes muscle degeneration and impaired platelet activation in mice.

Cell Calcium 2018 Dec 5;76:87-100. Epub 2018 Oct 5.

Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway. Electronic address:

STIM1 and ORAI1 regulate store-operated Ca entry (SOCE) in most cell types, and mutations in these proteins have deleterious and diverse effects. We established a mouse line expressing the STIM1 R304 W gain-of-function mutation causing Stormorken syndrome to explore effects on organ and cell physiology. While STIM1 R304 W was lethal in the homozygous state, surviving mice presented with reduced growth, skeletal muscle degeneration, and reduced exercise endurance. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183016
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http://dx.doi.org/10.1016/j.ceca.2018.10.001DOI Listing
December 2018
5 Reads

The multifaceted STAT3: How a transcription factor regulates Ca signaling via a degradative pathway.

Authors:
Jan B Parys

Cell Calcium 2018 Dec 12;76:137-139. Epub 2018 Oct 12.

KU Leuven, Laboratory for Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine & Leuven Kanker Instituut, Campus Gasthuisberg O/N-1 B-802, Herestraat 49, BE-3000 Leuven, Belgium. Electronic address:

STAT3 is a pleiotropic prosurvival transcription factor with functions in nucleus and mitochondria. Avalle et al. (Cell Death Diff. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183018
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http://dx.doi.org/10.1016/j.ceca.2018.10.002DOI Listing
December 2018
4 Reads

Transcriptomic changes in C2C12 myotubes triggered by electrical stimulation: Role of Ca-mediated and Ca-independent signaling and elevated [Na]/[K] ratio.

Cell Calcium 2018 Dec 30;76:72-86. Epub 2018 Sep 30.

M. V. Lomonosov Moscow State University, Moscow, Russia; National Research Tomsk State University, Tomsk, Russia; Siberian Medical State University, Tomsk, Russia. Electronic address:

Elevation of Ca and AMP-activated protein kinase (AMPK) are considered as major signals triggering transcriptomic changes in exercising skeletal muscle. Electrical pulse stimulation (EPS) of cultured myotubes is widely employed as an in vitro model of muscle contraction. This study examines the impact of Ca-mediated and Ca-independent signaling in transcriptomic changes in EPS-treated C2C12 myotubes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183008
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http://dx.doi.org/10.1016/j.ceca.2018.09.007DOI Listing
December 2018
1 Read

Lysosomal exocytosis of ATP is coupled to P2Y receptor in marginal cells in the stria vascular in neonatal rats.

Cell Calcium 2018 Dec 21;76:62-71. Epub 2018 Sep 21.

Department of Otorhinolaryngology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address:

Adenosine triphosphate (ATP) is stored as lysosomal vesicles in marginal cells of the stria vascular in neonatal rats, but the mechanisms of ATP release are unclear. Primary cultures of marginal cells from 1-day-old Sprague-Dawley rats were established. P2Y receptor and inositol 1,4,5-trisphosphate (IP3) receptor were immunolabelled in marginal cells of the stria vascular. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.006DOI Listing
December 2018
3.513 Impact Factor

Nonlinear relationship between ER Ca depletion versus induction of the unfolded protein response, autophagy inhibition, and cell death.

Cell Calcium 2018 Dec 17;76:48-61. Epub 2018 Sep 17.

Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership for Molecular Medicine, University of Oslo, Norway. Electronic address:

Endoplasmic reticulum (ER) Ca depletion activates the unfolded protein response (UPR), inhibits bulk autophagy and eventually induces cell death in mammalian cells. However, the extent and duration of ER Ca depletion required is unknown. We instigated a detailed study in two different cell lines, using sarco/endoplasmic reticulum Ca-ATPase (SERCA) inhibitors to gradually reduce ER Ca levels in a controlled manner. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.005DOI Listing
December 2018
8 Reads

Spontaneous Ca transients in rat pulmonary vein cardiomyocytes are increased in frequency and become more synchronous following electrical stimulation.

Cell Calcium 2018 Dec 16;76:36-47. Epub 2018 Sep 16.

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK. Electronic address:

The pulmonary veins have an external sleeve of cardiomyocytes that are a widely recognised source of ectopic electrical activity that can lead to atrial fibrillation. Although the mechanisms behind this activity are currently unknown, changes in intracellular calcium (Ca) signalling are purported to play a role. Therefore, the intracellular Ca concentration was monitored in the pulmonary vein using fluo-4 and epifluorescence microscopy. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.001DOI Listing
December 2018
8 Reads

Data-driven modeling of mitochondrial dysfunction in Alzheimer's disease.

Cell Calcium 2018 Dec 12;76:23-35. Epub 2018 Sep 12.

Department of Physics, University of South Florida, Tampa, FL 33620, USA. Electronic address:

Intracellular accumulation of oligomeric forms of β amyloid (Aβ) are now believed to play a key role in the earliest phase of Alzheimer's disease (AD) as their rise correlates well with the early symptoms of the disease. Extensive evidence points to impaired neuronal Ca homeostasis as a direct consequence of the intracellular Aβ oligomers. However, little is known about the downstream effects of the resulting Ca rise on the many intracellular Ca-dependent pathways. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289702PMC
December 2018
9 Reads

Key residues controlling bidirectional ion movements in Na/Ca exchanger.

Cell Calcium 2018 Dec 15;76:10-22. Epub 2018 Sep 15.

Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, 69978, Israel. Electronic address:

Prokaryotic and eukaryotic Na/Ca exchangers (NCX) control Ca homeostasis. NCX orthologs exhibit up to 10-fold differences in their turnover rates (k), whereas the ratios between the cytosolic (cyt) and extracellular (ext) K values (K = K/K) are highly asymmetric and alike (K ≤ 0.1) among NCXs. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.004DOI Listing
December 2018

Gain-of-function mutations in STIM1 and ORAI1 causing tubular aggregate myopathy and Stormorken syndrome.

Cell Calcium 2018 Dec 3;76:1-9. Epub 2018 Sep 3.

Departement of Translational Medicine and Neurogenetics, IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), Inserm U1258, CNRS UMR7104, Université de Strasbourg, Illkirch, France.

Calcium (Ca) is a key regulator for a large number of cellular functions in all kinds of cells, and small disturbances of Ca homeostasis can severely compromise normal physiology in various tissues and organs. A major mechanism controlling Ca homeostasis is store-operated Ca entry (SOCE), which relies on the concerted action of the reticular Ca sensor STIM1 and the plasma membrane Ca channel ORAI1. Gain-of-function mutations in the respective genes induce excessive Ca entry, and cause tubular aggregate myopathy (TAM) and Stormorken syndrome. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183007
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http://dx.doi.org/10.1016/j.ceca.2018.07.008DOI Listing
December 2018
3 Reads

Regulation of proto-oncogene Orai3 by miR18a/b and miR34a.

Cell Calcium 2018 Nov 3;75:101-111. Epub 2018 Sep 3.

CSIR- Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110025, India. Electronic address:

Store Operated Ca Entry (SOCE) mediated by Orai channels is a ubiquitous Ca influx pathway that regulates several cellular functions. We have earlier reported that Orai3, the mammalian specific Orai1 homolog, plays a critical role in breast cancer progression. More recently, Orai3 was demonstrated to regulate prostate and lung tumorigenesis. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.006DOI Listing
November 2018
3.513 Impact Factor

Calmodulin-binding proteins: A journey of 40 years.

Cell Calcium 2018 Nov 5;75:89-100. Epub 2018 Sep 5.

Department of Pathology & Laboratory Medicine, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon S7N 5E5, Canada.

The proteins which bind to calmodulin in a Ca-dependent and reversible manner are known as calmodulin-binding proteins. These proteins are involved in a multitude of processes in which Ca and calmodulin play crucial roles. Our group elucidated the mechanism and importance of these proteins in normal and diseased conditions. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.09.002DOI Listing
November 2018

CRAC channel-based optogenetics.

Cell Calcium 2018 Nov 3;75:79-88. Epub 2018 Sep 3.

Center for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, Houston, TX 77030, USA; Department of Medical Physiology, College of Medicine, Texas A&M University, Temple, TX 76504, USA. Electronic address:

Store-operated Ca² entry (SOCE) constitutes a major Ca influx pathway in mammals to regulate a myriad of physiological processes, including muscle contraction, synaptic transmission, gene expression, and metabolism. In non-excitable cells, the Ca² release-activated Ca² (CRAC) channel, composed of ORAI and stromal interaction molecules (STIM), constitutes a prototypical example of SOCE to mediate Ca entry at specialized membrane contact sites (MCSs) between the endoplasmic reticulum (ER) and the plasma membrane (PM). The key steps of SOCE activation include the oligomerization of the luminal domain of the ER-resident Ca sensor STIM1 upon Ca² store depletion, subsequent signal propagation toward the cytoplasmic domain to trigger a conformational switch and overcome the intramolecular autoinhibition, and ultimate exposure of the minimal ORAI-activating domain to directly engage and gate ORAI channels in the plasma membrane. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183012
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http://dx.doi.org/10.1016/j.ceca.2018.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176914PMC
November 2018
9 Reads

Comprehensive analysis of the roles of 'black' and 'gray' clusters in structure and function of rat β-parvalbumin.

Cell Calcium 2018 Nov 27;75:64-78. Epub 2018 Aug 27.

Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia; Department of Biomedical Engineering, Pushchino State Institute of Natural Sciences, Pushchino, Moscow Region, 142290, Russia.

Recently we found two highly conserved structural motifs in the proteins of the EF-hand calcium binding protein family. These motifs provide a supporting scaffold for the Ca binding loops and contribute to the hydrophobic core of the EF-hand domain. Each structural motif forms a cluster of three amino acids called cluster I ('black' cluster) and cluster II ('grey' cluster). Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.005DOI Listing
November 2018
2 Reads
3.513 Impact Factor

Residues important for Ca ion transport in the neuronal K-dependent Na-Ca exchanger (NCKX2).

Cell Calcium 2018 Sep 30;74:187-197. Epub 2018 Jun 30.

Department of Physiology and Pharmacology, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:

K-dependent Na-Ca exchangers (NCKXs) belong to Ca/cation antiporter gene superfamily. NCKX proteins play an important role in Ca homeostasis and are bi-directional plasma membrane Ca-transporters which utilize the inward Na and outward K gradients to move Ca ions into and out of the cytosol (4Na:1Ca + 1 K). In this study, we examined residues in the two regions with the highest degree of homology between the different NCKX isoforms (α-1 and α-2 repeats) to determine which residues are important for Ca coordination. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183004
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http://dx.doi.org/10.1016/j.ceca.2018.06.002DOI Listing
September 2018
9 Reads

How cellular Zn signaling drives physiological functions.

Cell Calcium 2018 Nov 18;75:53-63. Epub 2018 Aug 18.

Department of Physiology and Cell Biology and The Zlotowski Center for Neuroscience, Faculty of Health Sciences, POB 653, Ben-Gurion Ave., Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel. Electronic address:

Zinc is an essential micronutrient affecting many aspects of human health. Cellular Zn homeostasis is critical for cell function and survival. Zn, acting as a first or second messenger, triggers signaling pathways that mediate the physiological roles of Zn. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.004DOI Listing
November 2018
1 Read

A screening campaign in sea urchin egg homogenate as a platform for discovering modulators of NAADP-dependent Ca signaling in human cells.

Cell Calcium 2018 Nov 16;75:42-52. Epub 2018 Aug 16.

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee WI 53226, USA. Electronic address:

The Ca mobilizing second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) regulates intracellular trafficking events, including translocation of certain enveloped viruses through the endolysosomal system. Targeting NAADP-evoked Ca signaling may therefore be an effective strategy for discovering novel antivirals as well as therapeutics for other disorders. To aid discovery of novel scaffolds that modulate NAADP-evoked Ca signaling in human cells, we have investigated the potential of using the sea urchin egg homogenate system for a screening campaign. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286156PMC
November 2018

NAADP-dependent Ca signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system.

Cell Calcium 2018 Nov 9;75:30-41. Epub 2018 Aug 9.

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee WI 53226, USA. Electronic address:

Middle East Respiratory Syndrome coronavirus (MERS-CoV) infections are associated with a significant mortality rate, and existing drugs show poor efficacy. Identifying novel targets/pathways required for MERS infectivity is therefore important for developing novel therapeutics. As an enveloped virus, translocation through the endolysosomal system provides one pathway for cellular entry of MERS-CoV. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251489PMC
November 2018
12 Reads
3.510 Impact Factor

Smooth muscle gap-junctions allow propagation of intercellular Ca waves and vasoconstriction due to Ca based action potentials in rat mesenteric resistance arteries.

Cell Calcium 2018 Nov 7;75:21-29. Epub 2018 Aug 7.

Department of Cellular and Molecular Physiology and Gastroenterology, Institute of Translational Medicine, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK. Electronic address:

The role of vascular gap junctions in the conduction of intercellular Ca and vasoconstriction along small resistance arteries is not entirely understood. Some depolarizing agents trigger conducted vasoconstriction while others only evoke a local depolarization. Here we use a novel technique to investigate the temporal and spatial relationship between intercellular Ca signals generated by smooth muscle action potentials (APs) and vasoconstriction in mesenteric resistance arteries (MA). Read More

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http://dx.doi.org/10.1016/j.ceca.2018.08.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169741PMC
November 2018

CRAC channels in dental enamel cells.

Cell Calcium 2018 Nov 9;75:14-20. Epub 2018 Aug 9.

Dept. Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, New York 10010, USA. Electronic address:

Enamel mineralization relies on Ca availability provided by Ca release activated Ca (CRAC) channels. CRAC channels are modulated by the endoplasmic reticulum Ca sensor STIM1 which gates the pore subunit of the channel known as ORAI1, found the in plasma membrane, to enable sustained Ca influx. Mutations in the STIM1 and ORAI1 genes result in CRAC channelopathy, an ensemble of diseases including immunodeficiency, muscular hypotonia, ectodermal dysplasia with defects in sweat gland function and abnormal enamel mineralization similar to amelogenesis imperfecta (AI). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183011
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http://dx.doi.org/10.1016/j.ceca.2018.07.012DOI Listing
November 2018
6 Reads

Corrigendum to "Calcium mediates cell shape change in human peritoneal mesothelial cells" [Cell Calcium 72 (2018) 116-126].

Authors:
Stephen D Bird

Cell Calcium 2018 Sep 9;74:186. Epub 2018 Aug 9.

Department of Obstetrics and Gynaecology, The University of Melbourne, Victoria, Australia; Department of Medicine, Dunedin School of Medicine, The University of Otago, Dunedin, New Zealand. Electronic address:

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http://dx.doi.org/10.1016/j.ceca.2018.07.007DOI Listing
September 2018

Modulation of P2X7 purinergic receptor activity by extracellular Zn in cultured mouse hippocampal astroglia.

Cell Calcium 2018 Nov 30;75:1-13. Epub 2018 Jul 30.

Department of Pharmacology, Institute of Experimental Medicine of the Hungarian Academy of Sciences, Szigony utca 43, HU-1083 Budapest, Hungary.

The P2X7R protein, a P2 type purinergic receptor functioning as a non-selective cation channel, is expressed in different cell types of the central nervous system in several regions of the brain. The activation of the P2X7R protein by ATP modulates excitatory neurotransmission and contributes to microglial activation, apoptosis and neuron-glia communication. Zinc is an essential micronutrient that is highly concentrated in the synaptic vesicles of glutamatergic hippocampal neurons where free zinc ions released into the synaptic cleft alter glutamatergic signal transmission. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.010DOI Listing
November 2018

Calcium release-activated calcium channels and pain.

Cell Calcium 2018 Sep 29;74:180-185. Epub 2018 Jul 29.

Department of Anesthesiology, Rutgers New Jersey Medical School, Newark, NJ 07103, United States. Electronic address:

Calcium release-activated calcium (CRAC) channels are unique among ion channels that are activated in response to depletion of intracellular calcium stores and are highly permeable to Ca compared to other cations. CRAC channels mediate an important calcium signal for a wide variety of cell types and are well studied in the immune system. They have been implicated in a number of disorders such as immunodeficiency, musculosketal disorders and cancer. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119525PMC
September 2018

Heterogeneity of calcium clock functions in dormant, dysrhythmically and rhythmically firing single pacemaker cells isolated from SA node.

Cell Calcium 2018 Sep 10;74:168-179. Epub 2018 Jul 10.

Laboratory of Cardiovascular Science, National Institute on Aging, NIH, Biomedical Research Center, 251 Bayview Blvd. Suite 100, Baltimore, MD 21224-6825, USA. Electronic address:

Current understanding of how cardiac pacemaker cells operate is based mainly on studies in isolated single sinoatrial node cells (SANC), specifically those that rhythmically fire action potentials similar to the in vivo behavior of the intact sinoatrial node. However, only a small fraction of SANC exhibit rhythmic firing after isolation. Other SANC behaviors have not been studied. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.002DOI Listing
September 2018
2 Reads

ORAI channels and cancer.

Cell Calcium 2018 Sep 31;74:160-167. Epub 2018 Jul 31.

The School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia; Mater Research Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia. Electronic address:

Cancer is a major cause of death. The diversity of cancer types and the propensity of cancers to acquire resistance to therapies, including new molecularly targeted and immune-based therapies, drives the search for new ways to understand cancer progression. The remodelling of calcium (Ca) signalling and the role of the Ca signal in controlling key events in cancer cells such as proliferation, invasion and the acquisition of resistance to cell death pathways is well established. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.011DOI Listing
September 2018

CRAC channels as targets for drug discovery and development.

Cell Calcium 2018 Sep 18;74:147-159. Epub 2018 Jul 18.

CalciMedica, Inc., 505 Coast Blvd. South, Suite 202, La Jolla, CA 92037, United States. Electronic address:

Calcium release-activated calcium (CRAC) channels have been the target of drug discovery for many years. The identification of STIM and Orai proteins as key components of CRAC channels greatly facilitated this process because their co-expression in cell lines produced electrophysiological currents (I) much larger than those in native cells, making it easier to confirm and characterize the effects of modulatory compounds. A driving force in the quest for CRAC channel drugs has been the immunocompromised phenotype displayed by humans and mice with null or loss-of-function mutations in STIM1 or Orai1, suggesting that CRAC channel inhibitors could be useful therapeutics for autoimmune or inflammatory conditions. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.005DOI Listing
September 2018
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Revelation of an enigmatic signaling machinery-First insights into the mammalian TRPC architecture.

Cell Calcium 2018 Sep 19;74:144-146. Epub 2018 Jul 19.

Gottfried-Schatz-Research-Center - Biophysics, Medical University of Graz, Neue Stiftingtalstrasse 6/D04, 8010 Graz, Austria.

Canonical TRP channels (TRPCs) are a particularly enigmatic family of signaling molecules with multimodal sensing features, being involved in a wide range of biological functions. Until very recently, the main hurdle towards comprehensive mechanistic understanding of TRPC signaling has been the lack of structural information. This has changed early this year by several reports on TRPC architectures resolved by single particle cryo-EM analysis. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.006DOI Listing
September 2018

STIM- and Orai-mediated calcium entry controls NF-κB activity and function in lymphocytes.

Cell Calcium 2018 Sep 10;74:131-143. Epub 2018 Jul 10.

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. Electronic address:

The central role of Ca signaling in the development of functional immunity and tolerance is well established. These signals are initiated by antigen binding to cognate receptors on lymphocytes that trigger store operated Ca entry (SOCE). The underlying mechanism of SOCE in lymphocytes involves TCR and BCR mediated activation of Stromal Interaction Molecule 1 and 2 (STIM1/2) molecules embedded in the ER membrane leading to their activation of Orai channels in the plasma membrane. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01434160183008
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http://dx.doi.org/10.1016/j.ceca.2018.07.003DOI Listing
September 2018
6 Reads

Reduced calcium influx in the hypoxia-tolerant Spalax: The role of the erythropoietin receptor.

Cell Calcium 2018 Sep 17;74:123-130. Epub 2018 Jul 17.

Institute of Evolution, University of Haifa, Haifa, Israel; Department of Evolutionary and Environmental Biology, University of Haifa, Haifa, Israel. Electronic address:

Tissue hypoxia is a condition that induces calcium influx into living cells. Calcium is a major player in maintaining cell signaling and homeostasis, and mediates the regulation of gene transcription and cell proliferation; however, acute and aggressive calcium influx induced by hypoxia eventually leads to programmed cell death. The blind mole rat, Spalax, is a wild-spread burrowing mammal adapted to hypoxic environments. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.004DOI Listing
September 2018

Characterization and potential roles of calretinin in rodent spermatozoa.

Cell Calcium 2018 Sep 1;74:94-101. Epub 2018 Jun 1.

Laboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de Bruxelles, Brussels, Belgium. Electronic address:

Calretinin has been detected in various excitable cells but the presence and putative roles of such a calcium-binding protein has never been characterized in sperm. Epididymal spermatozoa were collected from C57Bl6 (wild-type, WT) or calretinin knockout (CR) mice and Wistar rats. A specific staining for calretinin was detected by immunofluorescence in the principal piece of the flagellum, both in WT mouse and rat spermatozoa. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.05.008DOI Listing
September 2018
1 Read

Dysfunction of SERCA pumps as novel mechanism of methylglyoxal cytotoxicity.

Cell Calcium 2018 Sep 19;74:112-122. Epub 2018 Jun 19.

Institute of Experimental Pharmacology and Toxicology, Centre of Experimental Medicine of the Slovak Academy of Sciences, Dubravska Cesta 9, 841 04 Bratislava, Slovak Republic. Electronic address:

A novel pathway of methylglyoxal (MGX)-induced apoptosis via sarcoplasmic reticulum Ca-ATPase (SERCA) is presented. Interaction of SERCA1 with MGX was investigated by molecular docking and experimentally in a cell-free system. MGX concentration- and time-dependently decreased SERCA1 activity. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.06.003DOI Listing
September 2018
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Neuronal calcium signaling via store-operated channels in health and disease.

Cell Calcium 2018 Sep 7;74:102-111. Epub 2018 Jul 7.

International Institute of Molecular and Cell Biology, Warsaw, Poland. Electronic address:

Store-operated calcium entry (SOCE) is the flow of calcium ions (Ca) into cells in response to the depletion of intracellular Ca stores that reside predominantly in the endoplasmic reticulum (ER). The role of SOCE has been relatively well understood for non-excitable cells. It is mediated mostly by the ER Ca sensor STIM1 and plasma membrane Ca channel Orai1 and serves to sustain Ca signaling and refill ER Ca stores. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.07.001DOI Listing
September 2018
9 Reads

Molecular regulation of MCU: Implications in physiology and disease.

Cell Calcium 2018 Sep 27;74:86-93. Epub 2018 Jun 27.

Department of Medical Genetics and Molecular Biochemistry, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA; Center for Translational Medicine, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, 19140, USA; Center for Precision Medicine, Department of Medicine, University of Texas Health San Antonio, San Antonio, Texas 78229. Electronic address:

Ca flux across the inner mitochondrial membrane (IMM) regulates cellular bioenergetics, intra-cellular cytoplasmic Ca signals, and various cell death pathways. Ca entry into the mitochondria occurs due to the highly negative membrane potential (ΔΨ) through a selective inward rectifying MCU channel. In addition to being regulated by various mitochondrial matrix resident proteins such as MICUs, MCUb, MCUR1 and EMRE, the channel is transcriptionally regulated by upstream Ca cascade, post transnational modification and by divalent cations. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.06.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119482PMC
September 2018
18 Reads

A simple end-point assay for calcium channel activity.

Cell Calcium 2018 Sep 1;74:73-85. Epub 2018 Jun 1.

Molecular Neurobiology Division, Rajiv Gandhi Centre for Biotechnology, Thycaud, P. O., Thiruvananthapuram, 695014, Kerala, India. Electronic address:

Cellular calcium signaling events are transient. Hence they are observed in real time using fluorescence imaging or electrophysiological methods that require sophisticated instrumentation and specialized skills. For high throughput assays simple and inexpensive techniques are desirable. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.05.009DOI Listing
September 2018
17 Reads

Residues important for K ion transport in the K-dependent Na-Ca exchanger (NCKX2).

Cell Calcium 2018 Sep 25;74:61-72. Epub 2018 Jun 25.

Department of Physiology and Pharmacology, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:

K-dependent Na-Ca exchangers (NCKXs) play an important role in Ca homeostasis in many tissues. NCKX proteins are bi-directional plasma membrane Ca-transporters which utilize the inward Na and outward K gradients to move Ca ions into and out of the cytosol (4Na:1Ca + 1 K). In this study, we carried out scanning mutagenesis of all the residues of the highly conserved α-1 and α-2 repeats of NCKX2 to identify residues important for K transport. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.06.005DOI Listing
September 2018
9 Reads