3,236 results match your criteria Cell Calcium[Journal]


Oxytocin prevents neuronal network pain-related changes on spinal cord dorsal horn in vitro.

Cell Calcium 2020 Jun 18;90:102246. Epub 2020 Jun 18.

Departamento de Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus Juriquilla, Boulevard Juriquilla, No. 3001, C.P. 76230, Querétaro, Mexico. Electronic address:

Recently, oxytocin (OT) has been studied as a potential modulator of endogenous analgesia by acting upon pain circuits at the spinal cord and supraspinal levels. Yet the detailed action mechanisms of OT are still undetermined. The present study aimed to evaluate the action of OT in the spinal cord dorsal horn network under nociceptive-like conditions induced by the activation of the N-methyl-d-aspartate (NMDA) receptor and formalin injection, using calcium imaging techniques. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102246DOI Listing

Ca signaling of human pluripotent stem cells-derived cardiomyocytes as compared to adult mammalian cardiomyocytes.

Cell Calcium 2020 Jun 13;90:102244. Epub 2020 Jun 13.

Cardiac Signaling Center of University of South Carolina, Medical University of South Carolina, Clemson University, Charleston SC, United States. Electronic address:

Human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CMs) have been extensively used for in vitro modeling of human cardiovascular disease, drug screening and pharmacotherapy, but little rigorous studies have been reported on their biophysical or Ca signaling properties. There is also considerable concern as to the level of their maturity and whether they can serve as reliable models for adult human cardiac myocytes. Ultrastructural difference such as lack of t-tubular network, their polygonal shapes, disorganized sarcomeric myofilament, and their rhythmic automaticity, among others, have been cited as evidence for immaturity of hiPSC-CMs. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102244DOI Listing

Natural photoswitches expose STIM1 activation steps.

Cell Calcium 2020 Jun 13;90:102240. Epub 2020 Jun 13.

Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria. Electronic address:

STIM1, an ER-located Ca2+ sensor, activates Orai1 channels upon Ca2+-storedepletion. Prior to this, STIM1 undergoes a sequence of conformational changes, which cannot be controlled individually with high spatiotemporal resolution. Ma et al. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102240DOI Listing

Calsequestrin. Structure, function, and evolution.

Cell Calcium 2020 Jun 20;90:102242. Epub 2020 Jun 20.

Department of Biochemistry, University of Alberta, Edmonton, AB, T6H 2S7, Canada. Electronic address:

Calsequestrin is the major Ca binding protein in the sarcoplasmic reticulum (SR), serves as the main Ca storage and buffering protein and is an important regulator of Ca release channels in both skeletal and cardiac muscle. It is anchored at the junctional SR membrane through interactions with membrane proteins and undergoes reversible polymerization with increasing Ca concentration. Calsequestrin provides high local Ca at the junctional SR and communicates changes in luminal Ca concentration to Ca release channels, thus it is an essential component of excitation-contraction coupling. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102242DOI Listing

To B, or not to B: Is calcium the answer?

Cell Calcium 2020 Jun 3;90:102227. Epub 2020 Jun 3.

Department of Pathology, New York University School of Medicine, New York, NY, USA. Electronic address:

B lymphocytes are an important component of the adaptive and innate immune system because of their ability to secrete antibodies and to present antigens to T cells, which is critical for immune responses to many pathogens. Abnormal B cell function is the cause of diseases including autoimmune, paraneoplastic, and immunodeficiency disorders. The development, survival, and function of B cells depend on signaling through the B cell receptor (BCR) and costimulatory receptors. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102227DOI Listing

BRAF and NRAS mutated melanoma: Different Ca responses, Na/Ca exchanger expression, and sensitivity to inhibitors.

Cell Calcium 2020 Jun 8;90:102241. Epub 2020 Jun 8.

Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC (UFABC), Santo André, SP, Brazil. Electronic address:

Calcium is a ubiquitous intracellular second messenger, playing central roles in the regulation of several biological processes. Alterations in Ca homeostasis and signaling are an important feature of tumor cells to acquire proliferative and survival advantages, which include structural and functional changes in storage capacity, channels, and pumps. Here, we investigated the differences in Ca homeostasis in vemurafenib-responsive and non-responsive melanoma cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102241DOI Listing

Pivotal role of type-1 inositol 1,4,5-trisphosphate receptor for glucagon-induced gluconeogenesis.

Cell Calcium 2020 Jun 10;90:102243. Epub 2020 Jun 10.

Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, 14642, United States. Electronic address:

We highlight a recent paper which documents the important role that Ca release through type-1 Inositol 1,4,5-trisphosphate receptor (IPR1) plays in the acute regulation by glucagon of gluconeogenesis in hepatocytes. The specificity is likely the result of discrete localization close to mitochondria and PKA-dependent phosphorylation of IPR1 which enhances Ca release. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102243DOI Listing

Calcium activates purified human TRPA1 with and without its N-terminal ankyrin repeat domain in the absence of calmodulin.

Cell Calcium 2020 Jun 8;90:102228. Epub 2020 Jun 8.

Department of Clinical Sciences Malmö, Lund University, SE-214 28, Malmö, Sweden. Electronic address:

Extracellular influx of calcium or release of calcium from intracellular stores have been shown to activate mammalian TRPA1 as well as to sensitize and desensitize TRPA1 electrophilic activation. Calcium binding sites on both intracellular N- and C-termini have been proposed. Here, we demonstrate based on Förster resonance energy transfer (FRET) and bilayer patch-clamp studies, a direct calmodulin-independent action of calcium on the purified human TRPA1 (hTRPA1), causing structural changes and activation without immediate subsequent desensitization of hTRPA1 with and without its N-terminal ankyrin repeat domain (N-ARD). Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102228DOI Listing

GCaMP as an indirect measure of electrical activity in rat trigeminal ganglion neurons.

Cell Calcium 2020 Jul 30;89:102225. Epub 2020 May 30.

Department of Neurobiology and the Pittsburgh Center for Pain Research, University of Pittsburgh, United States. Electronic address:

While debate continues over whether somatosensory information is transmitted via labeled line, population coding, frequency coding, or some combination therein, researchers have begun to address this question at the level of the primary afferent by using optical approaches that enable the assessment of neural activity in hundreds to even thousands of neurons simultaneously. However, with limited availability of tools to optically assess electrical activity in large populations of neurons, researchers have turned to genetically encoded Ca indicators (GECIs) including GCaMP to enable the detection of increases in cytosolic Ca concentrations as a correlate for neuronal activity. One of the most widely used GECIs is GCaMP6, which is available in three different versions tuned for sensitivity (GCaMP6s), speed (GCaMP6f), or a balance of the two (GCaMP6m). Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331800PMC

Ca signaling and lipid transfer 'pas a deux' at ER-PM contact sites orchestrate cell migration.

Authors:
Khaled Machaca

Cell Calcium 2020 Jul 1;89:102226. Epub 2020 Jun 1.

Department of Physiology and Biophysics, Ca(2+) Signaling Group, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, PO Box 24144, Doha, Qatar. Electronic address:

Contact sites between the endoplasmic reticulum (ER) and plasma membrane (PM) regulate both non-vesicular lipid transfer as well as Ca signaling with multiple interactions between the two pathways. Here I discuss recent findings that offer exciting insights into the role of store-operated Ca entry (SOCE), Oxysterol-binding protein (OSBP)-related proteins ORP3, Arf5 and the Arf GEF IQSec1 in this crosstalk and how they regulate cell migration and focal adhesion disassembly. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102226DOI Listing

Cytosolic Ca oversees the MASs production of pyruvate for the mitochondrial market.

Authors:
Martijn Kerkhofs

Cell Calcium 2020 Jul 3;89:102223. Epub 2020 Jun 3.

Laboratory for Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine and Leuven Kanker Instituut, KU Leuven, Leuven, Belgium. Electronic address:

It is generally accepted that mitochondrial Ca controls the pace of mitochondrial bioenergetics and thus ATP production. Szibor et al. challenge this paradigm, proposing that the balance between ATP consumption and production depends on mitochondrial pyruvate supply via the malate-aspartate shuttle (MAS) and is controlled by cytosolic Ca. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102223DOI Listing

PunctaSpecks: A tool for automated detection, tracking, and analysis of multiple types of fluorescently labeled biomolecules.

Cell Calcium 2020 Jul 25;89:102224. Epub 2020 May 25.

Department of Physics, University of South Florida, Tampa, FL 33647, USA. Electronic address:

Recent advances in imaging technology and fluorescent probes have made it possible to gain information about the dynamics of subcellular processes at unprecedented spatiotemporal scales. Unfortunately, a lack of automated tools to efficiently process the resulting imaging data encoding fine details of the biological processes remains a major bottleneck in utilizing the full potential of these powerful experimental techniques. Here we present a computational tool, called PunctaSpecks, that can characterize fluorescence signals arising from a wide range of biological molecules under normal and pathological conditions. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102224DOI Listing
July 2020
3.513 Impact Factor

Targeting mitochondrial calcium pathways as a potential treatment against Parkinson's disease.

Cell Calcium 2020 Jul 16;89:102216. Epub 2020 May 16.

Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Warsaw, Poland. Electronic address:

Parkinson's disease (PD) is a major health problem worldwide affecting millions of people and is a result of neurodegeneration in a small part of the brain known as substantia nigra pars compacta. Aberration in mitochondrial Ca homeostasis plays, among several other factors, an important role for the neuronal loss in PD. Mitochondria are vital for cellular physiology, e. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102216DOI Listing

The name tells the story: Two-pore channels.

Cell Calcium 2020 Jul 11;89:102215. Epub 2020 May 11.

Institute of Biochemistry and Molecular Medicine, National Center of Competence in Research NCCR TransCure, University of Bern, Bern, Switzerland. Electronic address:

TPC2-A1-N and TPC2-A1-P, two novel small molecules, differentially activate two-pore channel 2 (TPC2) and mimic the activation of TPC2 with NAADP and PIP, resulting in distinct ion channel selectivities. These two different modes of TPC2 activity have physiological, and possibly pathophysiological, implications as they can modulate vesicle trafficking and lysosomal exocytosis. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102215DOI Listing

Ca3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II.

Cell Calcium 2020 Jul 11;89:102214. Epub 2020 May 11.

Universidad Nacional Autónoma de México, Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Cuernavaca Morelos, 62210, México. Electronic address:

There is growing evidence indicating that the pore structure of voltage-gated ion channels (VGICs) influences gating besides their conductance. Regarding low voltage-activated (LVA) Ca channels, it has been demonstrated that substitutions of the pore aspartate (D) by a glutamate (D-to-E substitution) in domains III and IV alter channel gating properties such as a positive shift in the channel activation voltage dependence. In the present report, we evaluated the effects of E-to-D substitution in domains I and II on the Ca3. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102214DOI Listing

Regulation of the Ca-activated chloride channel Anoctamin-1 (TMEM16A) by Ca-induced interaction with FKBP12 and calcineurin.

Cell Calcium 2020 Jul 8;89:102211. Epub 2020 May 8.

Physics Institute, Universidad Autónoma de San Luis Potosí, Ave. Dr. Manuel Nava #6, San Luis Potosí, SLP 78290, Mexico. Electronic address:

Chloride fluxes through the calcium-gated chloride channel Anoctamin-1 (TMEM16A) control blood pressure, secretion of saliva, mucin, insulin, and melatonin, gastrointestinal motility, sperm capacitation and motility, and pain sensation. Calcium activates a myriad of regulatory proteins but how these proteins affect TMEM16A activity is unresolved. Here we show by co-immunoprecipitation that increasing intracellular calcium with ionomycin or by activating sphingosine-1-phosphate receptors, induces coupling of calcium/calmodulin-dependent phosphatase calcineurin and prolyl isomerase FK506-binding protein 12 (FKBP12) to TMEM16A in HEK-293 cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102211DOI Listing

4-chloro-orto-cresol activates ryanodine receptor more selectively and potently than 4-chloro-meta-cresol.

Cell Calcium 2020 Jun 1;88:102213. Epub 2020 May 1.

Department of Physiology, Faculty of Medicine, University of Debrecen, 98. Nagyerdei krt, Debrecen, Hungary. Electronic address:

In this study we performed the comprehensive pharmacological analysis of two stereoisomers of 4-chloro-meta-cresol (4CMC), a popular ryanodine receptor (RyR) agonist used in muscle research. Experiments investigating the Ca-releasing action of the isomers demonstrated that the most potent isomer was 4-chloro-orto-cresol (4COC) (EC = 55 ± 14 μM), although 3-chloro-para-cresol (3CPC) was more effective, as it was able to induce higher magnitude of Ca flux from isolated terminal cisterna vesicles. Nevertheless, 3CPC stimulated the hydrolytic activity of the sarcoplasmic reticulum ATP-ase (SERCA) with an EC of 91 ± 17 μM, while 4COC affected SERCA only in the millimolar range (IC = 1370 ± 88 μM). Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102213DOI Listing

The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system.

Cell Calcium 2020 Jun 30;88:102207. Epub 2020 Apr 30.

Department of Biology, University of Copenhagen, 13 Universitetsparken, DK-2100 Copenhagen, Denmark; MonTa Biosciences ApS, Diplomvej 381 2800 Lyngby, Denmark(1).

Calmodulin (CaM) is the principle mediator of the Ca signal in all eukaryotic cells. A huge variety of basic cellular processes including cell cycle control, proliferation, secretion and motility, among many others are governed by CaM, which regulates activities of myriads of target proteins. Mammalian CaM is encoded by three genes localized on different chromosomes all producing an identical protein. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102207DOI Listing

Could an endo-lysosomal ion channel be the Achilles heel of SARS-CoV2?

Cell Calcium 2020 May 6;88:102212. Epub 2020 May 6.

Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, Ludwig-Maximilians-Universität, Munich, Germany.

The ongoing SARS-CoV2 outbreak has developed into a global pandemic. Despite previous outbreaks of SARS-CoV and the related MERS-CoV in recent years, neither a vaccine nor any other medication for an effective treatment are currently available. Endo-lysosomal two-pore cation channels have now emerged as potential novel targets for SARS-CoV treatment. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201244PMC

A STIMulating journey into optogenetic engineering.

Cell Calcium 2020 May 4;88:102197. Epub 2020 May 4.

Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA. Electronic address:

Genetically-encoded calcium actuators (GECAs) stemmed from STIM1 have enabled optical activation of endogenous ORAI1 channels in both excitable and non-excitable tissues. These GECAs offer new non-invasive means to probe the structure-function relations of calcium channels and wirelessly control the behavior of awake mice. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102197DOI Listing

Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury.

Cell Calcium 2020 Apr 28;88:102198. Epub 2020 Apr 28.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address:

TRPV1 and TRPA1 are cation channels that play key roles in inflammatory signaling pathways. They are co-expressed on airway C-fibers, where they exert synergistic effects on causing inflammation and cough. Licorice, the root of Glycyrrhiza uralensis, has been widely used in China as an anti-inflammatory and anti-coughing herb. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102198DOI Listing

Mending Fences: Na,K-ATPase signaling via Ca in the maintenance of epithelium integrity.

Cell Calcium 2020 Apr 26;88:102210. Epub 2020 Apr 26.

Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Sweden.

Na,K-ATPase is a ubiquitous multifunctional protein that acts both as an ion pump and as a signal transducer. The signaling function is activated by ouabain in non-toxic concentrations. In epithelial cells the ouabain-bound Na,K-ATPase connects with the inositol 1,4,5-trisphosphate receptor via a short linear motif to activate low frequency Ca2+ oscillations. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102210DOI Listing
April 2020
3.513 Impact Factor

Multiple facets of TRPML1 in autophagy.

Cell Calcium 2020 Apr 27;88:102196. Epub 2020 Apr 27.

Departments of Physiology and Biophysics, Dalhousie University, 5850 College Street, Halifax, B3H 4R2, Nova Scotia, Canada. Electronic address:

Autophagy is an evolutionarily conserved pathway that is required for cellular homeostasis, growth and survival. In a recent study, Scotto-Rosato et al. demonstrate that TRPML1-mediated calcium release promotes autophagosome biogenesis by activating the CaMKKβ/VPS34 pathway, providing a new insight into the pathophysiological role of TRPML1 in human diseases. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102196DOI Listing

Parenchymal pressure injury Ca entry mechanism in pancreatitis.

Authors:
Robert Sutton

Cell Calcium 2020 Apr 19;88:102208. Epub 2020 Apr 19.

Liverpool Pancreatitis Research Group, Institute of Translational Medicine, University of Liverpool and Liverpool University Hospitals NHS Foundation Trust, Liverpool, UK. Electronic address:

Pancreatic trauma and high parenchymal pressure have been found to open Piezo1 plasma membrane Ca channels to such an extent that transient receptor potential vanilloid type 4 channels also open to further Ca entry, overloading the cell with Ca to induce acute pancreatitis (Swain et al, J Clin Invest, 2020). Elucidation of this mechanism is an important step in understanding pancreatitis and opens up new possibilities for prevention and treatment. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102208DOI Listing

In Memoriam Sir Michael Berridge 1938 - 2020.

Cell Calcium 2020 Apr 21;88:102209. Epub 2020 Apr 21.

Laboratory of Sensory Processing, Brain Mind Institute, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

The article is an 'In Memoriam' article honouring the memory of Sir Michael Berridge. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102209DOI Listing

Sumoylation of sodium/calcium exchanger in brain ischemia and ischemic preconditioning.

Cell Calcium 2020 May 16;87:102195. Epub 2020 Mar 16.

IRCCS SDN, Naples, Italy.

The small ubiquitin-like modifier (SUMO) conjugation (or SUMOylation) is a post-translational protein modification mechanism activated by different stress conditions that has been recently investigated in experimental models of cerebral ischemia. The expression of SUMOylation enzymes and substrates is not restricted to the nucleus, since they are present also in the cytoplasm and on plasma membrane and are involved in several physiological and pathological conditions. In the last decades, convincing evidence have supported the idea that the increased levels of SUMOylated proteins may induce tolerance to ischemic stress. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102195DOI Listing
May 2020
3.513 Impact Factor

Helix 8 - Putting a spring in mechano-sensing.

Authors:
Maike D Glitsch

Cell Calcium 2020 May 12;87:102192. Epub 2020 Mar 12.

Department of Physiology, Anatomy and Genetics, University of Oxford, UK. Electronic address:

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http://dx.doi.org/10.1016/j.ceca.2020.102192DOI Listing

TRP Channel: The structural era.

Cell Calcium 2020 May 4;87:102191. Epub 2020 Mar 4.

Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ceca.2020.102191DOI Listing

The Na/Caexchanger in Alzheimer's disease.

Cell Calcium 2020 May 2;87:102190. Epub 2020 Mar 2.

IRCSS SDN, Via Gianturco, 80131, Naples, Italy.

As a pivotal player in regulating sodium (Na) and calcium (Ca) homeostasis and signalling in excitable cells, the Na/Ca exchanger (NCX) is involved in many neurodegenerative disorders in which an imbalance of intracellular Ca and/or Na concentrations occurs, including Alzheimer's disease (AD). Although NCX has been mainly implicated in neuroprotective mechanisms counteracting Ca dysregulation, several studies highlighted its role in the neuronal responses to intracellular Na elevation occurring in several pathophysiological conditions. Since the alteration of Na and Ca homeostasis significantly contributes to synaptic dysfunction and neuronal loss in AD, it is of crucial importance to analyze the contribution of NCX isoforms in the homeostatic responses at neuronal and synaptic levels. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102190DOI Listing

Genetically modified mice to unravel physiological and pathophysiological roles played by NCX isoforms.

Cell Calcium 2020 May 2;87:102189. Epub 2020 Mar 2.

IRCSS SDN, Via Gianturco, 80131, Naples, Italy.

Since the discovery of the three isoforms of the Na/Ca exchanger, NCX1, NCX2 and NCX3 in 1990s, many studies have been devoted to identifying their specific roles in different tissues under several physiological or pathophysiological conditions. In particular, several seminal experimental works laid the foundation for better understanding gene and protein structures, tissue distribution, and regulatory functions of each antiporter isoform. On the other hand, despite the efforts in the development of specific compounds selectively targeting NCX1, NCX2 or NCX3 to test their physiological or pathophysiological roles, several drawbacks hampered the achievement of these goals. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102189DOI Listing

Nuclear-encoded NCX3 and AKAP121: Two novel modulators of mitochondrial calcium efflux in normoxic and hypoxic neurons.

Cell Calcium 2020 May 6;87:102193. Epub 2020 Mar 6.

Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of Medicine, Federico II University of Naples, Italy. Electronic address:

Mitochondria are highly dynamic organelles extremely important for cell survival. Their structure resembles that of prokaryotic cells since they are composed with two membranes, the inner (IMM) and the outer mitochondrial membrane (OMM) delimitating the intermembrane space (IMS) and the matrix which contains mitochondrial DNA (mtDNA). This structure is strictly related to mitochondrial function since they produce the most of the cellular ATP through the oxidative phosphorylation which generate the electrochemical gradient at the two sides of the inner mitochondrial membrane an essential requirement for mitochondrial function. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102193DOI Listing

Reliable measurement of free Ca concentrations in the ER lumen using Mag-Fluo-4.

Cell Calcium 2020 May 6;87:102188. Epub 2020 Mar 6.

Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.

Synthetic Ca indicators are widely used to report changes in free [Ca], usually in the cytosol but also within organelles. Mag-Fluo-4, loaded into the endoplasmic reticulum (ER) by incubating cells with Mag-Fluo-4 AM, has been used to measure changes in free [Ca] within the ER, where the free [Ca] is estimated to be between 100 μM and 1 mM. Many results are consistent with Mag-Fluo-4 reliably reporting changes in free [Ca] within the ER, but the results are difficult to reconcile with the affinity of Mag-Fluo-4 for Ca measured in vitro (K ∼22 μM). Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181174PMC

Transcriptional and epigenetic regulation of ncx1 and ncx3 in the brain.

Cell Calcium 2020 May 7;87:102194. Epub 2020 Mar 7.

IRCCS SDN Naples, Via Emanuele Gianturco 113, 80143, Naples, Italy.

Sodium-calcium exchanger (NCX) 1 and 3, have been demonstrated to play a relevant role in controlling the intracellular homeostasis of sodium and calcium ions in physiological and patho-physiological conditions. While NCX1 and NCX3 knocking-down have been both implicated in brain ischemia, several aspects of the epigenetic regulation of these two antiporters transcription were not yet well characterized. In response to stroke, NCX1 and NCX3 transcriptional regulation occurs from specific promoter sequences. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102194DOI Listing

Ca influx and clearance at hyperpolarized membrane potentials modulate spontaneous and stimulated exocytosis in neuroendocrine cells.

Cell Calcium 2020 May 24;87:102184. Epub 2020 Feb 24.

Department of Physiology and Membrane Biology, University of California, Davis, Davis, CA, 95616, USA. Electronic address:

Neuroendocrine adrenal chromaffin cells release neurohormones catecholamines in response to Ca entry via voltage-gated Ca channels (VGCCs). Adrenal chromaffin cells also express non-voltage-gated channels, which may conduct Ca at negative membrane potentials, whose role in regulation of exocytosis is poorly understood. We explored how modulation of Ca influx at negative membrane potentials affects basal cytosolic Ca concentration ([Ca]) and exocytosis in metabolically intact voltage-clamped bovine adrenal chromaffin cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102184DOI Listing

TRPM7 activation potentiates SOCE in enamel cells but requires ORAI.

Cell Calcium 2020 May 28;87:102187. Epub 2020 Feb 28.

Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY, 10010, USA. Electronic address:

Calcium (Ca) release-activated Ca (CRAC) channels mediated by STIM1/2 and ORAI (ORAI1-3) proteins form the dominant store-operated Ca entry (SOCE) pathway in a wide variety of cells. Among these, the enamel-forming cells known as ameloblasts rely on CRAC channel function to enable Ca influx, which is important for enamel mineralization. This key role of the CRAC channel is supported by human mutations and animal models lacking STIM1 and ORAI1, which results in enamel defects and hypomineralization. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202080PMC

Sodium/calcium exchanger as main effector of endogenous neuroprotection elicited by ischemic tolerance.

Cell Calcium 2020 May 17;87:102183. Epub 2020 Feb 17.

Division of Pharmacology, Department of Neuroscience, School of Medicine, University of Naples Federico II, Via Pansini, 5, 80131, Naples, Italy.

The ischemic tolerance (IT) paradigm represents a fundamental cell response to certain types or injury able to render an organ more "tolerant" to a subsequent, stronger, insult. During the 16th century, the toxicologist Paracelsus described for the first time the possibility that a noxious event might determine a state of tolerance. This finding was summarized in one of his most important mentions: "The dose makes the poison". Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102183DOI Listing

A trio has turned into a quartet: DJ-1 interacts with the IP3R-Grp75-VDAC complex to control ER-mitochondria interaction.

Cell Calcium 2020 May 24;87:102186. Epub 2020 Feb 24.

Department of Biology, University of Padova, Padova, Italy. Electronic address:

The outer mitochondrial membrane protein VDAC interacts with the ER protein IP3R via chaperone Grp75 to form a molecular complex that couples mitochondria to the ER and contributes to functional mitochondria-ER contacts (MERCs), essential for efficient calcium (Ca) transfer. A new study by Liu et al. identifies the PD protein DJ-1 as a component of the IP3R-Grp75-VDAC complex. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102186DOI Listing

Mouse S100G protein exhibits properties characteristic of a calcium sensor.

Cell Calcium 2020 May 24;87:102185. Epub 2020 Feb 24.

Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.

Bovine S100 G (calbindin D, small Ca-binding protein of the EF-hand superfamily) is considered as a calcium buffer protein; i.e., the binding of Ca practically does not change its general conformation. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102185DOI Listing
May 2020
3.513 Impact Factor

New perspectives for selective NCX activators in neurodegenerative diseases.

Cell Calcium 2020 May 7;87:102170. Epub 2020 Feb 7.

Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatologic Sciences, School of Medicine, "Federico II" University of Naples, 80131 Naples, Italy. Electronic address:

The Na/Ca exchanger plays a relevant role in several neurological disorders, thus the pharmacological modulation of its isoforms might represent a promising strategy to ameliorate the course of some neurological pathologies including stroke, neonatal hypoxia, multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), Alzheimer Disease (AD), and spinal muscular atrophy (SMA). This review will summarize heterocyclic, peptidergic, genetic and epigenetic compounds activating or inhibiting the expression/activity of each NCX isoform. In addition, we will focus our attention on the development of new strategies aimed to ameliorate the pathophysiological conditions in which NCX isoform changes are found. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102170DOI Listing

Single-channel properties of skeletal muscle ryanodine receptor pore ΔFF in two brothers with a lethal form of fetal akinesia.

Cell Calcium 2020 May 17;87:102182. Epub 2020 Feb 17.

Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599-7260, United States. Electronic address:

Ryanodine receptor ion channels (RyR1s) release Ca ions from the sarcoplasmic reticulum to regulate skeletal muscle contraction. By whole-exome sequencing, we identified the heterozygous RYR1 variant c.14767_14772del resulting in the in-frame deletion p. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216825PMC

Divalent cation influx and calcium homeostasis in germinal vesicle mouse oocytes.

Cell Calcium 2020 May 22;87:102181. Epub 2020 Feb 22.

Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, 661 North Pleasant Street, Amherst, MA, 01003, USA. Electronic address:

Prior to maturation, mouse oocytes are arrested at the germinal vesicle (GV) stage during which they experience constitutive calcium (Ca) influx and spontaneous Ca oscillations. The oscillations cease during maturation but Ca influx continues, as the oocytes' internal stores attain maximal content at the culmination of maturation, the metaphase II stage. The identity of the channel(s) that underlie this Ca influx has not been completely determined. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153984PMC

The role of mitochondria in shaping odor responses in Drosophila melanogaster olfactory sensory neurons.

Cell Calcium 2020 May 11;87:102179. Epub 2020 Feb 11.

Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knöll- Str. 8, D-07745 Jena, Germany. Electronic address:

Insects detect volatile chemosignals with olfactory sensory neurons (OSNs) that express olfactory receptors. Among them, the most sensitive receptors are the odorant receptors (ORs), which form cation channels passing also Ca. Here, we investigate if and how odor-induced Ca signals in Drosophila melanogaster OSNs are controlled by intracellular Ca stores, especially by mitochondria. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102179DOI Listing

Cracking the code of sodium/calcium exchanger (NCX) gating: Old and new complexities surfacing from the deep web of secondary regulations.

Cell Calcium 2020 May 8;87:102169. Epub 2020 Feb 8.

Department of Biomedical Sciences and Public Health, School of Medicine, University "Politecnica delle Marche", Via Tronto 10/A, 60126, Ancona, Italy.

Cell membranes spatially define gradients that drive the complexity of biological signals. To guarantee movements and exchanges of solutes between compartments, membrane transporters negotiate the passages of ions and other important molecules through lipid bilayers. The Na/Ca exchangers (NCXs) in particular play central roles in balancing Na and Ca fluxes across diverse proteolipid borders in all eukaryotic cells, influencing cellular functions and fate by multiple means. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102169DOI Listing

Modulation of the cardiac Na-Ca exchanger by cytoplasmic protons: Molecular mechanisms and physiological implications.

Cell Calcium 2020 May 11;87:102140. Epub 2019 Dec 11.

Department of Anesthesiology and Perioperative Medicine, Division of Molecular Medicine, UCLA, Los Angeles, CA 90095, USA; Cardiovascular Research Laboratory, UCLA, Los Angeles, CA 90095, USA. Electronic address:

A precise temporal and spatial control of intracellular Ca concentration is essential for a coordinated contraction of the heart. Following contraction, cardiac cells need to rapidly remove intracellular Ca to allow for relaxation. This task is performed by two transporters: the plasma membrane Na-Ca exchanger (NCX) and the sarcoplasmic reticulum (SR) Ca-ATPase (SERCA). Read More

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http://dx.doi.org/10.1016/j.ceca.2019.102140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153995PMC

Fight or flight: The culprit is lurking in the neighbourhood.

Cell Calcium 2020 May 11;87:102180. Epub 2020 Feb 11.

Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK.

The fight-or-flight response is studied by all students of Physiology as a concerted bodily response to danger. Liu et al (2020) have now revealed its mechanism, after surveying the proteomic neighbourhood around the cardiac calcium channels in a study which is a tour-de-force of modern biological techniques. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102180DOI Listing

Rapamycin inhibits B-cell activating factor (BAFF)-stimulated cell proliferation and survival by suppressing Ca-CaMKII-dependent PTEN/Akt-Erk1/2 signaling pathway in normal and neoplastic B-lymphoid cells.

Cell Calcium 2020 May 7;87:102171. Epub 2020 Feb 7.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China. Electronic address:

B-cell activating factor (BAFF) is a crucial survival factor for B cells, and excess BAFF contributes to development of autoimmune diseases. Recent studies have shown that rapamycin can prevent BAFF-induced B-cell proliferation and survival, but the underlying mechanism remains to be elucidated. Here we found that rapamycin inhibited human soluble BAFF (hsBAFF)-stimulated cell proliferation by inducing G-cell cycle arrest, which was through downregulating the protein levels of CDK2, CDK4, CDK6, cyclin A, cyclin D1, and cyclin E. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168758PMC
May 2020
3.513 Impact Factor

Na/Ca exchange in the atrium: Role in sinoatrial node pacemaking and excitation-contraction coupling.

Cell Calcium 2020 May 30;87:102167. Epub 2020 Jan 30.

Smidt Heart Institute, Department of Cardiology, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA. Electronic address:

Na/Ca exchange is the dominant calcium (Ca) efflux mechanism in cardiac myocytes. Although our knowledge of exchanger function (NCX1 in the heart) was originally established using biochemical and electrophysiological tools such as cardiac sarcolemmal vesicles and the giant patch technique [1-4], many advances in our understanding of the physiological/pathophysiological roles of NCX1 in the heart have been obtained using a suite of genetically modified mice. Early mouse studies focused on modification of expression levels of NCX1 in the ventricles, with transgenic overexpressors, global NCX1 knockout (KO) mice (which were embryonic lethal if homozygous), and finally ventricular-specific NCX1 KO [5-12]. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153991PMC

Analysis of calcium signaling in live human Tongue cell 3D-Cultures upon tastant perfusion.

Cell Calcium 2020 May 23;87:102164. Epub 2020 Jan 23.

Institute of Molecular and Cell Biology, Hochschule Mannheim, 68163 Mannheim, Germany.

Bridging the gap between two-dimensional cell cultures and complex in vivo tissues, three-dimensional cell culture models are of increasing interest in the fields of cell biology and pharmacology. However, present challenges hamper live cell imaging of three-dimensional cell cultures. These include (i) the stabilization of these structures under perfusion conditions, (ii) the recording of many z-planes at high spatio-temporal resolution, (iii) and the data analysis that ranges in complexity from whole specimens to single cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102164DOI Listing

STIM1-Ca signaling in coronary sinus cardiomyocytes contributes to interatrial conduction.

Cell Calcium 2020 May 21;87:102163. Epub 2020 Jan 21.

Division of Cardiovascular Medicine, Department of Medicine, Duke University School of Medicine, Box 103031 Med Ctr, Durham, NC, 27710, United States. Electronic address:

Pacemaker action potentials emerge from the sinoatrial node (SAN) and rapidly propagate through the atria to the AV node via preferential conduction pathways, including one associated with the coronary sinus. However, few distinguishing features of these tracts are known. Identifying specific molecular markers to distinguish among these conduction pathways will have important implications for understanding atrial conduction and atrial arrhythmogenesis. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102163DOI Listing

Multipurpose Na ions mediate excitation and cellular homeostasis: Evolution of the concept of Na pumps and Na/Ca exchangers.

Cell Calcium 2020 May 25;87:102166. Epub 2020 Jan 25.

Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, M13 9PT, UK; Achucarro Centre for Neuroscience, IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain. Electronic address:

Ionic signalling is the most ancient form of regulation of cellular functions in response to environmental challenges. Signals, mediated by Na fluxes and spatio-temporal fluctuations of Na concentration in cellular organelles and cellular compartments contribute to the most fundamental cellular processes such as membrane excitability and energy production. At the very core of ionic signalling lies the Na-K ATP-driven pump (or NKA) which creates trans-plasmalemmal ion gradients that sustain ionic fluxes through ion channels and numerous Na-dependent transporters that maintain cellular and tissue homeostasis. Read More

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http://dx.doi.org/10.1016/j.ceca.2020.102166DOI Listing