3,013 results match your criteria Cell Calcium[Journal]


Spontaneous calcium transients in the immature adult-born neurons of the olfactory bulb.

Cell Calcium 2018 Jun 6;74:43-52. Epub 2018 Jun 6.

Institute of Physiology, Department of Neurophysiology, Eberhard Karls University of Tübingen, Tübingen, Germany. Electronic address:

Spontaneous neuronal activity and concomitant intracellular Ca signaling are abundant during early perinatal development and are well known for their key role in neuronal proliferation, migration, differentiation and wiring. However, much less is known about the in vivo patterns of spontaneous Ca signaling in immature adult-born cells. Here, by using two-photon Ca imaging, we analyzed spontaneous in vivo Ca signaling in adult-born juxtaglomerular cells of the mouse olfactory bulb over the time period of 5 weeks, from the day of their arrival in the glomerular layer till their stable integration into the preexisting neural network. Read More

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Somatostatin promotes glucose generation of Caoscillations in pancreatic islets both in the absence and presence of tolbutamide.

Cell Calcium 2018 Jun 1;74:35-42. Epub 2018 Jun 1.

Department of Medical Cell Biology, Uppsala University, Biomedicum Box 571, SE-75123 Uppsala, Sweden.

Many cellular processes, including pulsatile release of insulin, are triggered by increase of cytoplasmic Ca. This study examines how somatostatin affects glucose generation of cytoplasmic Ca oscillations in mouse islets in absence and presence of tolbutamide blockade of the K channels. Ca was measured with dual wavelength microflurometry in isolated islets loaded with the indicator Fura-2. Read More

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SNARE-mediated vesicle navigation, vesicle anatomy and exocytotic fusion pore.

Authors:
Robert Zorec

Cell Calcium 2018 Jul 29;73:53-54. Epub 2018 Mar 29.

Laboratory of Neuroendocrinology-Molecular Cell Physiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia; Celica BIOMEDICAL, Tehnološki park 24, 1000 Ljubljana, Slovenia. Electronic address:

The focus of this special issue (SI) »Membrane Merger in Conventional and Unconventional Vesicle Secretion« is regulated exocytosis, a universally conserved mechanism, consisting of a merger between the vesicle and the plasma membranes. Although this process evolved with eukaryotic organisms some three billion years ago (Spang et al., 2015), the understanding of physiology and patobiology of this process, especially at elementary vesicle level, remains unclear. Read More

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Ca-dependent regulation and binding of calmodulin to multiple sites of Transient Receptor Potential Melastatin 3 (TRPM3) ion channels.

Cell Calcium 2018 Jul 31;73:40-52. Epub 2018 Mar 31.

Experimentelle und Klinische Pharmakologie und Toxikologie, Universität des Saarlandes, 66421 Homburg, Germany. Electronic address:

TRPM3 proteins assemble to Ca-permeable cation channels in the plasma membrane, which act as nociceptors of noxious heat and mediators of insulin and cytokine release. Here we show that TRPM3 channel activity is strongly dependent on intracellular Ca. Conceivably, this effect is attributed to the Ca binding protein calmodulin, which binds to TRPM3 in a Ca-dependent manner. Read More

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The remembrance of the things past: Conserved signalling pathways link protozoa to mammalian nervous system.

Cell Calcium 2018 Jul 8;73:25-39. Epub 2018 Apr 8.

Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK; IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain; Department of Neurosciences, University of the Basque Country UPV/EHU, 48940, Leioa, Spain.

The aim of the present article is to analyse the evolutionary links between protozoa and neuronal and neurosecretory cells. To this effect we employ functional and topological data available for ciliates, in particular for Paramecium. Of note, much less data are available for choanoflagellates, the progenitors of metazoans, which currently are in the focus of metazoan genomic data mining. Read More

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July 2018
3 Reads

Three-dimensional spatio-temporal modelling of store operated Ca entry: Insights into ER refilling and the spatial signature of Ca signals.

Cell Calcium 2018 Jul 4;73:11-24. Epub 2018 Apr 4.

Centre for Mathematical Medicine and Biology, School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK.

The spatial organisation of Orai channels and SERCA pumps within ER-PM junctions is important for enhancing the versatility and specificity of sub-cellular Ca signals generated during store operated Ca entry (SOCE). In this paper, we present a novel three dimensional spatio-temporal model describing Ca dynamics in the ER-PM junction and sub-PM ER during SOCE. We investigate the role of Orai channel and SERCA pump location to provide insights into how these components shape the Ca signals generated and affect ER refilling. Read More

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α2 isoform of Na,K-ATPase via Na,Ca exchanger modulates myelin basic protein synthesis in oligodendrocyte lineage cells in vitro.

Cell Calcium 2018 Jul 31;73:1-10. Epub 2018 Mar 31.

Institute of Physiology, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, D-55128 Mainz, Germany. Electronic address:

Oligodendrocytes in the CNS myelinate neuronal axons, facilitating rapid propagation of action potentials. Myelin basic protein (MBP) is an essential component of myelin and its absence results in severe hypomyelination. In oligodendrocyte lineage cell (OLC) monocultures MBP synthesis starts at DIV4. Read More

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To die or not to die SGK1-sensitive ORAI/STIM in cell survival.

Cell Calcium 2018 May 3;74:29-34. Epub 2018 May 3.

German Center for Neurodegenerative Diseases, Research Site Tübingen, Germany; Department of Neurology and Hertie Institute for Clinical Brain Research, University of Tübingen, Germany.

The pore forming Ca release activated Ca channel (CRAC) isoforms ORAI1-3 and their regulators STIM1,2 accomplish store operated Ca entry (SOCE). Activation of SOCE may lead to cytosolic Ca oscillations, which in turn support cell proliferation and cell survival. ORAI/STIM and thus SOCE are upregulated by the serum and glucocorticoid inducible kinase SGK1, a kinase under powerful genomic regulation and activated by phosphorylation via the phosphoinositol-3-phosphate pathway. Read More

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Evidence for the interaction of peroxiredoxin-4 with the store-operated calcium channel activator STIM1 in liver cells.

Cell Calcium 2018 May 15;74:14-28. Epub 2018 May 15.

Discipline of Medical Biochemistry, College of Medicine and Public Health, Flinders University, Adelaide, South Australia, 5001, Australia. Electronic address:

Ca entry through store-operated Ca channels (SOCs) in the plasma membrane (PM) of hepatocytes plays a central role in the hormonal regulation of liver metabolism. SOCs are composed of Orai1, the channel pore protein, and STIM1, the activator protein, and are regulated by hormones and reactive oxygen species (ROS). In addition to Orai1, STIM1 also interacts with several other intracellular proteins. Read More

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May 2018
1 Read

A personal journey.

Authors:
James W Putney

Cell Calcium 2018 Jun 21;72:127-131. Epub 2018 May 21.

Scientist Emeritus, National Institute of Environmental Health Sciences - NIH, Research Triangle Park, NC 27709, USA.

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Calmodulins from Schistosoma mansoni: Biochemical analysis and interaction with IQ-motifs from voltage-gated calcium channels.

Cell Calcium 2018 May 17;74:1-13. Epub 2018 May 17.

School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK; Institute for Global Food Security, Queen's University Belfast, 18-30 Malone Road, Belfast BT9 5BN, UK; School of Pharmacy and Biomolecular Sciences, University of Brighton, Huxley Building, Lewes Road, Brighton BN2 4GJ, UK. Electronic address:

The trematode Schistosoma mansoni is a causative agent of schistosomiasis, the second most common parasitic disease of humans after malaria. Calcium homeostasis and calcium-mediated signalling pathways are of particular interest in this species. The drug of choice for treating schistosomiasis, praziquantel, disrupts the regulation of calcium uptake and there is interest in exploiting calcium-mediated processes for future drug discovery. Read More

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May 2018
4 Reads

Lnc'ing Ca, SERCA and cardiac disease.

Cell Calcium 2018 Jun 17;72:132-134. Epub 2018 May 17.

Laboratory of Experimental Cardiology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium. Electronic address:

Loss of SERCA function contributes to reduced contractility, Ca overload and arrhythmias in the diseased heart. A long non-coding RNA (ZFAS1) upregulated in cardiac disease is reported to directly inhibit SERCA function. The implications for cardiac disease and the wider roles of SERCA are discussed. Read More

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The still uncertain identity of the channel-forming unit(s) of the mitochondrial permeability transition pore.

Cell Calcium 2018 Jul 16;73:121-130. Epub 2018 May 16.

Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 Ciudad de México, Mexico. Electronic address:

Mitochondria from different organisms can undergo a sudden process of inner membrane unselective leakiness to molecules known as the mitochondrial permeability transition (MPT). This process has been studied for nearly four decades and several proteins have been claimed to constitute, or at least regulate the usually inactive pore responsible for this transition. However, no protein candidate proposed as the actual pore-forming unit has passed rigorous gain- or loss-of-function genetic tests. Read More

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The hidden potential of lysosomal ion channels: A new era of oncogenes.

Cell Calcium 2018 Jun 10;72:91-103. Epub 2018 Mar 10.

Departments of Physiology & Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada. Electronic address:

Lysosomes serve as the control centre for cellular clearance. These membrane-bound organelles receive biomolecules destined for degradation from intracellular and extracellular pathways; thus, facilitating the production of energy and shaping the fate of the cell. At the base of their functionality are the lysosomal ion channels which mediate the function of the lysosome through the modulation of ion influx and efflux. Read More

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Blocking IP signal transduction pathways inhibits melatonin-induced Ca signals and impairs P. falciparum development and proliferation in erythrocytes.

Cell Calcium 2018 Jun 14;72:81-90. Epub 2018 Mar 14.

New Jersey Medical School Rutgers, The State University of New Jersey, NJ, USA; Núcleo de Pesquisa em Sinalização Celular Patógeno-Hospedeiro (NUSCEP) Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. Electronic address:

Inositol 1,4,5 trisphosphate (IP) signaling plays a crucial role in a wide range of eukaryotic processes. In Plasmodium falciparum, IP elicits Ca release from intracellular Ca stores, even though no IP receptor homolog has been identified to date. The human host hormone melatonin plays a key role in entraining the P. Read More

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June 2018
1 Read

Oncogenic KRAS suppresses store-operated Ca entry and I through ERK pathway-dependent remodelling of STIM expression in colorectal cancer cell lines.

Cell Calcium 2018 Jun 14;72:70-80. Epub 2018 Mar 14.

Laboratory of Experimental Cardiology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium; Previously at Babraham Institute, Babraham Research Campus, Cambridge, UK. Electronic address:

The KRAS GTPase plays a fundamental role in transducing signals from plasma membrane growth factor receptors to downstream signalling pathways controlling cell proliferation, survival and migration. Activating KRAS mutations are found in 20% of all cancers and in up to 40% of colorectal cancers, where they contribute to dysregulation of cell processes underlying oncogenic transformation. Multiple KRAS-regulated cell functions are also influenced by changes in intracellular Ca levels that are concurrently modified by receptor signalling pathways. Read More

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Oxysterol-binding protein related-proteins (ORPs) 5 and 8 regulate calcium signaling at specific cell compartments.

Cell Calcium 2018 Jun 13;72:62-69. Epub 2018 Mar 13.

Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland; Minerva Foundation Institute For Medical Research, Biomedicum Helsinki, 00290 Helsinki, Finland. Electronic address:

Oxysterol-binding protein related-protein 5 and 8 (ORP5/8) localize to the membrane contact sites (MCS) of the endoplasmic reticulum (ER) and the mitochondria, as well as to the ER-plasma membrane (PM) MCS. The MCS are emerging as important regulators of cell signaling events, including calcium (Ca) signaling. ORP5/8 have been shown to interact with phosphatidylinositol-4,5-bisphosphate (PIP) in the PM, and to modulate mitochondrial respiration and morphology. Read More

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Retinoic acid inhibits neuronal voltage-gated calcium channels.

Cell Calcium 2018 Jun 13;72:51-61. Epub 2018 Feb 13.

Department of Biological Sciences, Brock University, 1812 Sir Isaac BrockWay, St Catharines, Ontario, L2S 3A1, Canada. Electronic address:

Retinoic acid is the active metabolite of vitamin A and regulates several important cellular processes by activating retinoic acid receptors (RAR) and retinoid X receptors (RXR). These receptors generally act as transcription factors, though non-genomic actions of both retinoic acid and the receptors have also been reported. One such nongenomic effect includes the modulation of Ca levels during homeostatic synaptic plasticity in the hippocampus. Read More

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Assessment of cytosolic free calcium changes during ceramide-induced cell death in MDA-MB-231 breast cancer cells expressing the calcium sensor GCaMP6m.

Cell Calcium 2018 Jun 2;72:39-50. Epub 2018 Mar 2.

School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia; Mater Research Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia. Electronic address:

Alterations in Ca signaling can regulate key cancer hallmarks such as proliferation, invasiveness and resistance to cell death. Changes in the regulation of intracellular Ca and specific components of Ca influx are a feature of several cancers and/or cancer subtypes, including the basal-like breast cancer subtype, which has a poor prognosis. The development of genetically encoded calcium indicators, such as GCaMP6, represents an opportunity to measure changes in intracellular free Ca during processes relevant to breast cancer progression that occur over long periods (e. Read More

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Underlying mechanism of the contractile dysfunction in atrophied ventricular myocytes from a murine model of hypothyroidism.

Cell Calcium 2018 Jun 5;72:26-38. Epub 2018 Feb 5.

Tecnologico de Monterrey, Escuela de Medicina, Av. Morones Prieto No. 3000 Pte., Monterrey, N.L., 64710, Mexico. Electronic address:

Hypothyroidism (Hypo) is a risk factor for cardiovascular diseases, including heart failure. Hypo rapidly induces Ca mishandling and contractile dysfunction (CD), as well as atrophy and ventricular myocytes (VM) remodeling. Hypo decreases SERCA-to-phospholamban ratio (SERCA/PLB), and thereby contributes to CD. Read More

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Effects of the iron chelator deferiprone and the T-type calcium channel blocker efonidipine on cardiac function and Ca regulation in iron-overloaded thalassemic mice.

Cell Calcium 2018 Jun 6;72:18-25. Epub 2018 Feb 6.

Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand; Cardiac Electrophysiology Unit, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand; Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address:

Although disturbance of cardiac Ca regulation is involved in the pathophysiology of iron-overload cardiomyopathy, the obvious mechanisms involved in the dysregulation of iron-induced cardiac Ca are unclear. Moreover, the roles of the iron chelator deferiprone and the T-type calcium channel blocker efonidipine on cardiac intracellular Ca transients and Ca regulatory proteins in thalassemic mice are still unknown. We tested the hypothesis that treatment with either deferiprone or efonidipine attenuated cardiac Ca dysregulation and led to improved left ventricular (LV) function in iron-overloaded thalassemic mice. Read More

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The sarco(endo)plasmic reticulum calcium ATPase SCA-1 regulates the Caenorhabditis elegans nicotinic acetylcholine receptor ACR-16.

Cell Calcium 2018 Jun 24;72:104-115. Epub 2018 Feb 24.

Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, United States.

Nicotinic acetylcholine receptors (nAChR) are present in many excitable tissues and are found both pre and post-synaptically. Through their non-specific cationic permeability, these nAChRs have excitatory roles in neurotransmission, neuromodulation, synaptic plasticity, and neuroprotection. Thus, nAChR mislocalization or functional deficits are associated with many neurological disease states. Read More

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The effects of mitochondrial inhibitors on Ca signalling and electrical conductances required for pacemaking in interstitial cells of Cajal in the mouse small intestine.

Cell Calcium 2018 Jun 1;72:1-17. Epub 2018 Feb 1.

Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA. Electronic address:

Interstitial cells of Cajal (ICC-MY) are pacemakers that generate and propagate electrical slow waves in gastrointestinal (GI) muscles. Slow waves appear to be generated by the release of Ca from intracellular stores and activation of Ca-activated Cl channels (Ano1). Conduction of slow waves to smooth muscle cells coordinates rhythmic contractions. Read More

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The early signaling pathway of live yeast cell derivative in THP-1 monocytes.

Cell Calcium 2018 Jul 22;73:112-120. Epub 2018 Apr 22.

Department of Molecular Genetics, Biochemistry & Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Live yeast cell derivative (LYCD) is a medicinal yeast extract that has been used in the treatment of burns, wounds and hemorrhoids for over 70 years. It has been shown to enhance the closure of skin wounds in diabetic mice by increasing inflammation, angiogenesis, formation of granulation tissue and epithelial migration. An active fraction of LYCD has been identified as a mixture of peptides ranging in size from 5 kDA to 17 kDA. Read More

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Calcium mediates cell shape change in human peritoneal mesothelial cells.

Authors:
Stephen D Bird

Cell Calcium 2018 Jun 21;72:116-126. Epub 2018 Feb 21.

Department of Obstetrics and Gynaecology, The University of Melbourne, Victoria, Australia; Department of Medicine, Dunedin School of Medicine, The University of Otago, Dunedin, New Zealand. Electronic address:

Mast cells in the peritoneal membrane (PM) may degranulate to release preformed inflammatory mediators including histamine which is capable of diffusing into the surrounding interstitium, modulating cells in their vicinity including, human peritoneal mesothelial cells (hPMC). The present study aimed to investigate the quorum intracellular calcium ([Ca]) response to histamine compared to the membrane soluble ionophore, A23187, in adherent cultured hPMC. To examine [Ca] handling, Fura - 2 loaded cells were exposed to histamine and A23187. Read More

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CRISPR/Cas9 Gene editing of RyR2 in human stem cell-derived cardiomyocytes provides a novel approach in investigating dysfunctional Ca signaling.

Cell Calcium 2018 Jul 27;73:104-111. Epub 2018 Apr 27.

Cardiac Signaling Center of University of South Carolina, Medical University of South Carolina, and Clemson University, Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, 29425, United States. Electronic address:

Type-2 ryanodine receptors (RyR2s) play a pivotal role in cardiac excitation-contraction coupling by releasing Ca from sarcoplasmic reticulum (SR) via a Ca -induced Ca release (CICR) mechanism. Two strategies have been used to study the structure-function characteristics of RyR2 and its disease associated mutations: (1) heterologous cell expression of the recombinant mutant RyR2s, and (2) knock-in mouse models harboring RyR2 point mutations. Here, we establish an alternative approach where Ca signaling aberrancy caused by the RyR2 mutation is studied in human cardiomyocytes with robust CICR mechanism. Read More

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Calcium- and ATP-dependent regulation of Na/Ca exchange function in BHK cells: Comparison of NCX1 and NCX3 exchangers.

Cell Calcium 2018 Jul 20;73:95-103. Epub 2018 Apr 20.

Department of Biomedical Science and Public Health, School of Medicine, University "Politecnica delle Marche", Ancona, Italy. Electronic address:

Na/Ca exchangers (NCX) mediate bidirectional Ca fluxes across cell membranes and contribute to Ca homeostasis in many cell types. Exchangers are regulated by gating reactions that depend on Na and Ca binding to transport and regulatory sites. A Na-dependent inactivation is prominent in all isoforms, whereas Ca-dependent regulation varies among isoforms. Read More

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Structural elements of stromal interaction molecule function.

Cell Calcium 2018 Jul 18;73:88-94. Epub 2018 Apr 18.

Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada. Electronic address:

Stromal interaction molecule (STIM)-1 and -2 are multi-domain, single-pass transmembrane proteins involved in sensing changes in compartmentalized calcium (Ca) levels and transducing this cellular signal to Orai1 channel proteins. Our understanding of the molecular mechanisms underlying STIM signaling has been dramatically improved through available X-ray crystal and solution NMR structures. This high-resolution structural data has revealed that intricate intramolecular and intermolecular protein-protein interactions are involved in converting STIMs from the quiescent to activation-competent states. Read More

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Deleterious effects of calcium indicators within cells; an inconvenient truth.

Cell Calcium 2018 Jul 15;73:82-87. Epub 2018 Apr 15.

KU Leuven, Laboratory of Molecular and Cellular Signaling, Department Cellular and Molecular Medicine, Campus Gasthuisberg O/N-I bus 802, Herestraat 49, BE-3000, Leuven, Belgium.

The study of cellular Ca signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca indicators, and briefly discusses some approaches that may mitigate against misleading outcomes. Read More

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Differential effects of lipopolysaccharide on mouse sensory TRP channels.

Cell Calcium 2018 Jul 14;73:72-81. Epub 2018 Apr 14.

Laboratory for Ion Channel Research, Department of Cellular and Molecular Medicine, KU Leuven, VIB Center for Brain & Disease Research, O&N1 Herestraat 49 - box 802, 3000, Leuven, Belgium. Electronic address:

Acute neurogenic inflammation and pain associated to bacterial infection have been traditionally ascribed to sensitization and activation of sensory nerve afferents secondary to immune cell stimulation. However, we recently showed that lipopolysaccharides (LPS) directly activate the Transient Receptor Potential channels TRPA1 in sensory neurons and TRPV4 in airway epithelial cells. Here we investigated whether LPS activates other sensory TRP channels expressed in sensory neurons. Read More

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GRAM marks the spot for STIM. Commentary on "GRAM domain proteins specialize functionally distinct ER-PM contact sites in human cells".

Cell Calcium 2018 Jul 18;73:70-71. Epub 2018 Apr 18.

Department of Cell Physiology and Metabolism, Rue Michel-Servet, 1, University of Geneva, Switzerland. Electronic address:

GRAM domain proteins were reported as novel ER-PM tethers defining specific membrane contact sites (MCS) subdomains. GRAMD2a pre-marks the sites occupied by STIM1 at MCS and its ablation impairs STIM1 translocation, but not store-operated Ca entry. We discuss these apparently counterintuitive findings in the context of STIM/ORAI signaling at MCS. Read More

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Photoreceptor calcium sensor proteins in detergent-resistant membrane rafts are regulated via binding to caveolin-1.

Cell Calcium 2018 Jul 14;73:55-69. Epub 2018 Apr 14.

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.

Rod cell membranes contain cholesterol-rich detergent-resistant membrane (DRM) rafts, which accumulate visual cascade proteins as well as proteins involved in regulation of phototransduction such as rhodopsin kinase and guanylate cyclases. Caveolin-1 is the major integral component of DRMs, possessing scaffolding and regulatory activities towards various signaling proteins. In this study, photoreceptor Ca-binding proteins recoverin, NCS1, GCAP1, and GCAP2, belonging to neuronal calcium sensor (NCS) family, were recognized as novel caveolin-1 interacting partners. Read More

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A critical appraisal of the role of intracellular Ca-signaling pathways in Kawasaki disease.

Cell Calcium 2018 May 3;71:95-103. Epub 2018 Feb 3.

KU Leuven, Laboratory of Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine, B-3000 Leuven, Belgium. Electronic address:

Kawasaki disease is a multi-systemic vasculitis that generally occurs in children and that can lead to coronary artery lesions. Recent studies showed that Kawasaki disease has an important genetic component. In this review, we discuss the single-nucleotide polymorphisms in the genes encoding proteins with a role in intracellular Ca signaling: inositol 1,4,5-trisphosphate 3-kinase C, caspase-3, the store-operated Ca-entry channel ORAI1, the type-3 inositol 1,4,5-trisphosphate receptor, the Na/Ca exchanger 1, and phospholipase Cß4 and Cß1. Read More

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May 2018
2 Reads

An improved measurement of the Ca-binding affinity of fluorescent Ca indicators.

Cell Calcium 2018 May 8;71:86-94. Epub 2018 Jan 8.

Eccles Institute of Neuroscience, JCSMR, ANU, Acton ACT, 2601, Australia; ANU Medical School, ANU, Acton ACT, 2601, Australia.

Fluorescent Ca indicators are widely used to measure the intracellular Ca concentration ([Ca]) in living cells, including neurons. By calibrating an indicator in solutions that mimic the main ionic constituents of the actual cytoplasm, [Ca] can be determined from the measured fluorescence intensity. However, different studies have reported considerably different Ca-binding affinities (K) for the same indicator, even though they used calibrating solutions with similar compositions. Read More

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May 2018
1 Read

Anoctamin 9/TMEM16J is a cation channel activated by cAMP/PKA signal.

Cell Calcium 2018 May 30;71:75-85. Epub 2017 Dec 30.

College of Pharmacy, Seoul National University, Seoul, 08826, Republic of Korea; Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea. Electronic address:

Anoctamins (ANOs) are multifunctional membrane proteins that consist of 10 homologs. ANO1 (TMEM16A) and ANO2 (TMEM16B) are anion channels activated by intracellular calcium that meditate numerous physiological functions. ANO6 is a scramblase that redistributes phospholipids across the cell membrane. Read More

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May 2018
5 Reads

Computational modeling of amylin-induced calcium dysregulation in rat ventricular cardiomyocytes.

Cell Calcium 2018 May 8;71:65-74. Epub 2017 Dec 8.

Department of Chemistry, University of Kentucky, 505 Rose St. Chemistry-Physics Building, Lexington, KY 40506, USA. Electronic address:

Hyperamylinemia is a condition that accompanies obesity and precedes type II diabetes, and it is characterized by above-normal blood levels of amylin, the pancreas-derived peptide. Human amylin oligomerizes easily and can deposit in the pancreas [1], brain [2], and heart [3], where they have been associated with calcium dysregulation. In the heart, accumulating evidence suggests that human amylin oligomers form moderately cation-selective [4,5] channels that embed in the cell sarcolemma (SL). Read More

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May 2018
1 Read

Selective inhibition of histamine-evoked Ca signals by compartmentalized cAMP in human bronchial airway smooth muscle cells.

Cell Calcium 2018 May 15;71:53-64. Epub 2017 Dec 15.

Department of Pharmacology,Tennis Court Road, Cambridge, CB2 1PD, UK. Electronic address:

Intracellular Ca and cAMP typically cause opposing effects on airway smooth muscle contraction. Receptors that stimulate these pathways are therapeutic targets in asthma and chronic obstructive pulmonary disease. However, the interactions between different G protein-coupled receptors (GPCRs) that evoke cAMP and Ca signals in human bronchial airway smooth muscle cells (hBASMCs) are poorly understood. Read More

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May 2018
2 Reads

STIM1 and TRPV4 regulate fluid flow-induced calcium oscillation at early and late stages of osteoclast differentiation.

Cell Calcium 2018 May 11;71:45-52. Epub 2017 Dec 11.

Beijing Institute of Technology, School of Aerospace Engineering, Beijing 100081, PR China. Electronic address:

Bone resorption is mainly mediated by osteoclasts (OCs), whose formation and function are regulated by intracellular Ca oscillation. Our previous studies demonstrated that fluid shear stress (FSS) lead to Ca oscillation through mechanosensitive cation-selective channels. However, the specific channels responsible for this FSS-induced Ca oscillation remain unknown. Read More

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May 2018
4 Reads

Dynamic Ca imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca puffs.

Cell Calcium 2018 May 1;71:34-44. Epub 2017 Dec 1.

Department of Neurobiology and Behavior, Department of Physiology and Biophysics, University of California, Irvine, CA 92697, United States. Electronic address:

We describe the construction of a simplified, inexpensive lattice light-sheet microscope, and illustrate its use for imaging subcellular Ca puffs evoked by photoreleased i-IP in cultured SH-SY5Y neuroblastoma cells loaded with the Ca probe Cal520. The microscope provides sub-micron spatial resolution and enables recording of local Ca transients in single-slice mode with a signal-to-noise ratio and temporal resolution (2ms) at least as good as confocal or total internal reflection microscopy. Signals arising from openings of individual IPR channels are clearly resolved, as are stepwise changes in fluorescence reflecting openings and closings of individual channels during puffs. Read More

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May 2018
3 Reads

Deletion of Orai2 augments endogenous CRAC currents and degranulation in mast cells leading to enhanced anaphylaxis.

Cell Calcium 2018 May 27;71:24-33. Epub 2017 Nov 27.

Pharmakologisches Institut, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany. Electronic address:

All three members of the Orai family of cation channels-Orai1, Orai2 and Orai3-are integral membrane proteins that can form store-operated Ca channels resembling endogenous calcium release-activated channels (CRAC) in many aspects. Loss of function studies in human and murine models revealed many functions of Orai1 proteins not only for Ca homeostasis, but also for cellular and systemic functions in many cell types. By contrast, the knowledge regarding the contribution of Orai2 and Orai3 proteins in these processes is sparse. Read More

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May 2018
2 Reads

The changes of intracellular calcium concentration and distribution in the hard end pear (Pyrus pyrifolia cv. 'Whangkeumbae') fruit.

Cell Calcium 2018 May 22;71:15-23. Epub 2017 Nov 22.

Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, College of Horticulture, Qingdao Agricultural University, No. 700 Changcheng Road, Chengyang, Qingdao City, 266109, Shandong Province, China.

Hard end is a physiological disorder of pear fruit that is frequently observed in the 'Whangkeumbae' (Pyrus pyrifolia) variety, however, the mechanisms that are involved in its development are poorly understood. In this study, we explored the causes of hard end disorder in pear fruit in relation to calcium deficiency. During fruit development, the ratio of Ca/N, Ca/K, Ca/Mg and the content of B were significantly lower in the hard end fruit as compared to normal fruit. Read More

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May 2018
1 Read

Coupling of P2Y receptors to Ca mobilization in mesenchymal stromal cells from the human adipose tissue.

Cell Calcium 2018 May 7;71:1-14. Epub 2017 Nov 7.

Institute of Cell Biophysics, Russian Academy of Sciences, Institutional Street 3, Pushchino, Moscow Region, 142290, Russia. Electronic address:

The purinergic transduction was examined in mesenchymal stromal cells (MSCs) from the human adipose tissue, and several nucleotides, including ATP, UTP, and ADP, were found to mobilize cytosolic Ca. Transcripts for multiple purinoreceptors were detected in MSC preparations, including A, A, A, P2Y, P2Y, P2Y, P2Y, P2Y, P2Y, P2Y, P2X, P2X, and P2X. Cellular responses to nucleotides were insignificantly sensitive to bath Ca, pointing at a minor contribution of Ca entry, and were suppressed by U73122 and 2-APB, implicating the phosphoinositide cascade in coupling P2Y receptors to Ca release. Read More

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May 2018
2 Reads

Ca signaling and cell death: Focus on the role of Ca signals in the regulation of cell death & survival processes in health, disease and therapy.

Cell Calcium 2018 Mar 22;70:1-2. Epub 2017 Nov 22.

KU Leuven, Laboratory of Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine and Leuven Kanker Instituut (LKI), Campus Gasthuisberg O&N1 - Box 802, Herestraat 49, B-3000, Leuven, Belgium. Electronic address:

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March 2018
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Augmented gene expression triggered by Na,K-ATPase inhibition: Role of Ca-mediated and -independent excitation-transcription coupling.

Cell Calcium 2017 Dec 9;68:5-13. Epub 2017 Oct 9.

Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia; Tomsk National Research University, Tomsk, Russia; Siberian Medical State University, Tomsk, Russia. Electronic address:

In rat vascular smooth muscle cells (RVSMC), 3-h Na,K-ATPase inhibition by ouabain or in K-free medium resulted in the inversion of the [Na]/[K] ratio and elevation up to 7-fold the content of Egr1, Atf3, Nr4a1 and Ptgs2 mRNAs. Ouabain increased the rate of 45Ca influx by 2-fold that was abolished by L-type voltage-gated Ca channel blocker nicardipine, but it was resistant to Na/Ca exchanger inhibitor KB-R7943. To study the role of Ca-mediated signaling in the expression of Na/K-sensitive genes we used intracellular Ca chelator BAPTA and incubated RVSMC in Ca-free medium. Read More

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December 2017
6 Reads

Fusion pore in exocytosis: More than an exit gate? A β-cell perspective.

Cell Calcium 2017 Dec 7;68:45-61. Epub 2017 Nov 7.

Laboratoire de Chimie et Biologie des Membranes et Nano-objets (CBMN), CNRS UMR 5248, Université de Bordeaux, Allée de Geoffrey St Hilaire, 33600 Pessac, France. Electronic address:

Secretory vesicle exocytosis is a fundamental biological event and the process by which hormones (like insulin) are released into the blood. Considerable progress has been made in understanding this precisely orchestrated sequence of events from secretory vesicle docked at the cell membrane, hemifusion, to the opening of a membrane fusion pore. The exact biophysical and physiological regulation of these events implies a close interaction between membrane proteins and lipids in a confined space and constrained geometry to ensure appropriate delivery of cargo. Read More

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December 2017
3 Reads

Differential cytotoxicity and intracellular calcium-signalling following activation of the calcium-permeable ion channels TRPV1 and TRPA1.

Cell Calcium 2017 Dec 18;68:34-44. Epub 2017 Oct 18.

Department of Anaesthesiology and Intensive Care Medicine, Hannover Medical School, Hannover, Germany. Electronic address:

Several members of the transient receptor channel (TRP) family can mediate a calcium-dependent cytotoxicity. In sensory neurons, vanilloids like capsaicin induce neurotoxicity by activating TRPV1. The closely related ion channel TRPA1 is also activated by irritants, but it is unclear if and how TRPA1 mediates cell death. Read More

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December 2017
7 Reads

The steroid hormone 20-hydroxyecdysone upregulates calcium release-activated calcium channel modulator 1 expression to induce apoptosis in the midgut of Helicoverpa armigera.

Cell Calcium 2017 Dec 20;68:24-33. Epub 2017 Oct 20.

Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China. Electronic address:

Animal steroid hormones stimulate extracellular Ca influx into cells; however, the mechanism remains unclear. In this study, we determined that the Ca influx induced by steroid hormone 20-hydroxyecdysone (20E) is mediated by the calcium release-activated calcium channel modulator 1 (CRACM1/Orai1). The Orai1 mRNA is highly expressed during midgut programmed cell death in the lepidopteran insect Helicoverpa armigera. Read More

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December 2017
12 Reads

Satnav for cells: Destination membrane fusion.

Authors:
Matthew Holt

Cell Calcium 2017 Dec 9;68:14-23. Epub 2017 Oct 9.

Laboratory of Glia Biology, VIB-KU Leuven Center for Brain and Disease Research, Herestraat 49, B-3000 Leuven, Belgium. Electronic address:

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December 2017
5 Reads

NAADP-evoked Ca signals through two-pore channel-1 require arginine residues in the first S4-S5 linker.

Cell Calcium 2017 Dec 29;68:1-4. Epub 2017 Sep 29.

Center for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA.

Two-pore channels (TPCs) are two-domain members of the voltage-gated ion channel superfamily that localize to acidic organelles. Their mechanism of activation (ligands such as NAADP/PI(3,5)P versus voltage) and ion selectivity (Ca versus Na) is debated. Here we report that a cluster of arginine residues in the first domain required for selective voltage-gating of TPC1 map not to the voltage-sensing fourth transmembrane region (S4) but to a cytosolic downstream region (S4-S5 linker). Read More

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December 2017
6 Reads

Calcium signaling at the endoplasmic reticulum: fine-tuning stress responses.

Cell Calcium 2018 Mar 20;70:24-31. Epub 2017 Aug 20.

Center for Geroscience, Brain Health and Metabolism, Faculty of Medicine, University of Chile, Chile; Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago, Chile; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, University of Chile, Santiago, Chile; Buck Institute for Research on Aging, Novato, CA, 94945, USA; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA. Electronic address:

Endoplasmic reticulum (ER) calcium signaling is implicated in a myriad of coordinated cellular processes. The ER calcium content is tightly regulated as it allows a favorable environment for protein folding, in addition to operate as a major reservoir for fast and specific release of calcium. Altered ER homeostasis impacts protein folding, activating the unfolded protein response (UPR) as a rescue mechanism to restore proteostasis. Read More

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March 2018
6 Reads