Augmented gene expression triggered by Na(+),K(+)-ATPase inhibition: Role of Ca(2+)i-mediated and -independent excitation-transcription coupling.
Cell Calcium 2017 Dec 9;68:5-13. Epub 2017 Oct 9.
Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia; Tomsk National Research University, Tomsk, Russia; Siberian Medical State University, Tomsk, Russia. Electronic address:
In rat vascular smooth muscle cells (RVSMC), 3-h Na(+),K(+)-ATPase inhibition by ouabain or in K(+)-free medium resulted in the inversion of the [Na(+)]i/[K(+)]i ratio and elevation up to 7-fold the content of Egr1, Atf3, Nr4a1 and Ptgs2 mRNAs. Ouabain increased the rate of 45Ca(2+) influx by 2-fold that was abolished by L-type voltage-gated Ca(2+) channel blocker nicardipine, but it was resistant to Na(+)/Ca(2+) exchanger inhibitor KB-R7943. To study the role of Ca(2+)-mediated signaling in the expression of Na(+)i/K(+)i-sensitive genes we used intracellular Ca(2+) chelator BAPTA and incubated RVSMC in Ca(2+)-free medium. Read More