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    3589 results match your criteria Cell Biology International [Journal]

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    Metabolic profiling of normal hepatocyte and hepatocellular carcinoma cells via (1) H nuclear magnetic resonance spectroscopy.
    Cell Biol Int 2017 Nov 16. Epub 2017 Nov 16.
    Department of Radiology, Fujian Provincial Cancer Hospital, Fuzhou, 350014, China.
    Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Read More

    Co-culturing porcine normal urothelial cells, urinary bladder fibroblasts and smooth muscle cells for tissue engineering research.
    Cell Biol Int 2017 Nov 8. Epub 2017 Nov 8.
    Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Slovenia.
    New strategies for culturing and co-culturing of the main types of urinary bladder cells are essential for successful establishment of biomimetic in vitro models, which could be applied for research into, and management of, diverse urological disorders. Porcine normal urothelial cells are available in nearly unlimited amounts and have many properties equivalent to human urothelial cells. In the present study, we established normal differentiated porcine urothelial cells in co-cultures with porcine urinary bladder normal fibroblasts and/or smooth muscle cells. Read More

    Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary.
    Cell Biol Int 2017 Nov 7. Epub 2017 Nov 7.
    Provincial Key Laboratory of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, #1 Beijing East Road, Wuhu, Anhui, 241000, P.R. China.
    The Drosophila ovary provides an attractive model for studying the extrinsic or intrinsic factors that regulate the fate of germline stem cells (GSCs). Using this model, we identified a new role for Drosophila spaghetti (spag), encoding a homolog of human RNA polymerase II-associated protein 3 (RPAP3), in regulating the fate of ovarian GSCs. Results from spag knockdown and genetic mosaic studies suggest that spag functions as an intrinsic factor for GSCs maintenance. Read More

    In vitro treatment with Concentrated Growth Factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines.
    Cell Biol Int 2017 Nov 4. Epub 2017 Nov 4.
    Division of Anatomy and Physiopathology, Department of Clinical and Experimental Sciences, University of Brescia, 25123 Brescia, Italy.
    The aim of this study was to investigate the in vitro effect of Silicon, in the soluble form of sodium orthosilicate, combined and not with the Concentrated Growth Factors (CGF), a platelet rich preparation, on three different human cell lines of fibroblasts (NHDF), endothelial cells (HUVEC) and osteoblasts (HOBs). Each cell type, was treated with sodium orthosilicate at the final concentration of 0,5 mM and 1 mM, CGF, and sodium orthosilicate combined with CGF, for 72 hours. At the end of the experimental period, the in vitro effect on cell growth, proliferation and metabolic activity was evaluated by performing a simple cell count, using an automated cell counter and by evaluating the expression of the intracellular proliferation marker Ki-67, using Fluorescence-activated cell sorting (FACS) analysis. Read More

    SODD promotes glucose uptake of colorectal cancer cells via AKT pathway.
    Cell Biol Int 2017 Oct 31. Epub 2017 Oct 31.
    Department of Gastric Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Insititute.
    Altered glucose metabolism has been found in a variety of malignant diseases and recognized as one of the hallmarks of cancer. However, the molecular mechanism behind it is far from well understood. In current study, we found silencer of death domains (SODD) regulates glucose uptake of colorectal cancer cells. Read More

    MiR-29b expression is associated with a dexmedetomidine-mediated protective effect against oxygen-glucose deprivation-induced injury to SK-N-SH cells in vitro.
    Cell Biol Int 2017 Oct 31. Epub 2017 Oct 31.
    The Key Laboratiory of Medical Molecular Biology in GuiZhou Medical University.
    Ischemic cerebral stroke is a leading cause of death and long-term disability world-wide. Neuronal injury following cerebral ischemia initiates a complex series of signaling cascades that lead to neuronal cell death. MicroRNA 29b (miR-29b) has reported involvement in the pathogenic process of ischemic brain injury. Read More

    Cancer and normal adipose-derived mesenchymal stem cells (ASCs): Do they have differential effects on tumor and immune cells?
    Cell Biol Int 2017 Oct 27. Epub 2017 Oct 27.
    Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
    Adipose-derived mesenchymal stem cells (ASCs) are known to have immunomodulatory properties through soluble factors or by direct cell-to-cell contact. This study aimed to assess the expression of HLA-G and IDO activity in breast cancer and normal ASCs and to see if ASC is capable of modulating both tumor cells and immune system cells in vitro. ASCs were enzymatically isolated from 15 breast cancer patients and 10 normal individuals. Read More

    Cell line cross-contamination: a detrimetal issue in current biomedical research.
    Cell Biol Int 2017 Oct 27. Epub 2017 Oct 27.
    Medicine Faculty, Biomedical Research Labs Catholic University of Mule, 3605 San Miguel Avenue Talca, Chile 167.
    In an article in the issue of September of Cell Biology International (Saladino et al. 2017), authors have used the cell line ECV-304 as a "human endothelial cell line". They even declare, as stated in text, that cell line were obtained from European Collection of Cell Cultures (ECACC), accordingly to the policy of the Journal. Read More

    Desipramine-induced lysosomal vacuolization is independent of autophagy.
    Cell Biol Int 2017 Oct 25. Epub 2017 Oct 25.
    Department of Internal Medicine, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
    Desipramine, a commonly used antidepressant drug, induced cytosolic vacuolization in L929 cells. The level of LC3-II was elevated and that of p62 was reduced in desipramine-treated L929 cells, indicating the induction of autophagy by desipramine. Surprisingly, massive vacuolization was observed in desipramine-treated L929 cells in the presence of LY294002, an inhibitor of autophagy. Read More

    Isolation and characterisation of human mesenchymal stem cells derived from synovial fluid by magnetic activated cell sorting (MACS).
    Cell Biol Int 2017 Oct 25. Epub 2017 Oct 25.
    Guangzhou Medical University, Guangzhou 510095, Guangdong Province, China.
    Mesenchymal stem cells (MSCs) are the primary source of cells used for cell-based therapy in tissue engineering. Mesenchymal stem cells (MSCs) are found in synovial fluid, a source that could be conveniently used for cartilage tissue engineering. However, the purification and characterisation of SF-MSCs has been poorly documented in the literature. Read More

    Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα-knockdown cells.
    Cell Biol Int 2017 Oct 25. Epub 2017 Oct 25.
    Department of Endodontics, School of Stomatology, China Medical University, Nanjing North Street 117, Shenyang, 110002, China.
    Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-related transcription factor 2 (Runx2). We previously reported that knockdown of PP2A Cα increases the expression of Osterix, leading to the accelerated osteoblast differentiation through the upregulation of bone-related genes. Read More

    The Micronome of Mesenchymal Stromal Cells is partially responsive to Inflammation.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Université Libre de Bruxelles (ULB), Campus Erasme, Brussels, Belgium.
    Mesenchymal stromal cells (MSCs) display a special immunological profile that allows their potential use as immunotherapeutic cells. Nowadays, Foreskin (FSK) represents a valuable reservoir of MSCs with International Society for cellular Therapy (ISCT) compliant criteria and relevant functional properties. However, their mode of action is poorly understood and needs to be more elucidated to optimize their therapeutic use. Read More

    Histone deacetylase inhibitor stimulates E2 and P4 secretion in sika deer ovarian granulosa cells at a moderate dose.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
    The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. Read More

    Contribution to mitochondrial research in Brazil: 10th anniversary of the mitomeeting.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Biology Institute, State University of Campinas, 13083-970, Campinas, SP, Brazil.
    This commentary introduces the subject, the context and the history of the Brazilian annually held meeting on Mitochondrial Research by the occasion of its 10th anniversary. Mitomeetings gather people interested in all aspects of mitochondrial biology in diverse species, including protists, animals, plants, and fungi. Read More

    miR-23b-3p and miR-125b-5p downregulate apo(a) expression by targeting Ets1 in HepG2 cells.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan, 421001, PR China.
    High concentrations of plasma lipoprotein(a) [Lp(a)] have been inferred to be an independent risk factor for cardiovascular and cerebrovascular diseases, such as coronary artery diseases, restenosis, and stroke. Apolipoprotein(a) [apo(a)] is one of the most important components of Lp(a) and contributes greatly to the increased concentration of plasma Lp(a). As a critical positive transacting factor of apo(a) gene, Ets1 has been proven as a target gene of several miRNAs, such as miR-193b, miR-125b-5p, miR-200b, miR-1, and miR-499. Read More

    OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, Jiangsu, 213164, P. R. China.
    4β-(1,3,4-oxadiazole-2-amino-5-methyl)-4-deoxypodophyllotoxin (OAMDP), a novel podophyllotoxin derivative, has demonstrated potent anti-tumor activity with significant cytotoxic effect. Here, we report the anti-proliferative effect of OAMDP, for which OAMDP could suppress the proliferation of HepG2 cells (human hepatoma cell line) in a dose- and time-dependent manner. After treating with OAMDP, cell apoptosis was confirmed by Annexin V-FITC/PI double staining assay. Read More

    HBxAg suppresses cell apoptosis and promotes the secretion of placental hormones in human placental trophoblasts via activation of the EGFR/Akt pathway.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    Department of Gynecology and Obstetrics, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China.
    The aim of this study is to investigate the role of Hepatitis B virus x (HBx) in the growth and secretion of human placental trophoblasts. Firstly, placenta tissues were collected from pregnant HBV carriers with various viral loads. The results of immunohistochemical technique showed that the HBx protein and pEGFR protein levels were both markedly increased with the viral load elevation. Read More

    Carnosine and N-acetyl cysteine protect against sodium nitrite-induced oxidative stress in rat blood.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    Faculty of Life Sciences, Department of Biochemistry, Aligarh Muslim University, Aligarh, 202002, UP, India.
    Sodium nitrite (NaNO2 ) is widely used in the food industry as a preservative and colorant in meat and fish products. Industrialization and improper agricultural practices have greatly increased human exposure to high nitrite levels, mainly through contaminated drinking water, causing various health disorders. We have investigated the protective effect of carnosine (CAR) and N-acetyl cysteine (NAC) on NaNO2 -induced toxicity in rat blood. Read More

    Src promotes EGF-induced epithelial-to-mesenchymal transition and migration in gastric cancer cells by upregulating ZEB1 and ZEB2 through AKT.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, Liaoning, 110001, P.R. China.
    Epithelial-to-mesenchymal transition (EMT) plays important roles in the migration, invasion, and metastasis of cancer cells. However, the role of Src in epidermal growth factor (EGF)-induced EMT and migration in gastric cancer cells remains to be clarified. In the current study, the effect of Src on EGF-stimulated EMT and migration was explored in gastric cancer cells. Read More

    A novel HIF1AN substrate KANK3 plays a tumor-suppressive role in hepatocellular carcinoma.
    Cell Biol Int 2017 Oct 19. Epub 2017 Oct 19.
    Department of Biomedical Sciences, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.
    The KN motif and ankyrin repeat domain-containing protein (KANK) family is involved in actin cytoskeleton organization and cell motility. Compared with other KANK members, the biological function of KANK3 is not clear. Here, we identified KANK3 as a new substrate for the oxygen sensor hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), which hydroxylates HIF-1/2α and other ankyrin repeat domain-containing proteins at asparagine residues. Read More

    High magnesium prevents matrix vesicle-mediated mineralization in human bone marrow-derived mesenchymal stem cells via mitochondrial pathway and autophagy.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
    Magnesium, as a physiological calcium antagonist, plays vital role in the bone metabolism and the balance between magnesium and calcium is crucial in bone physiology. We recently demonstrated that matrix mineralization in human bone marrow-derived mesenchymal stem cells (hBMSCs) can be suppressed by high Mg(2+) . However, a complete understanding of the mechanisms involved still remains to be elucidated. Read More

    Down-regulation of LncRNA CCAT1 enhances radiosensitivity via regulating miR-148b in breast cancer.
    Cell Biol Int 2017 Oct 11. Epub 2017 Oct 11.
    Department of Oncology, The People's Hospital of Jianyang City, No.180 Hospital Road, Sichuan, 641400, China.
    LncRNA colon-cancer-associated transcript-1 (CCAT1) was proved to be a potential prognostic biomarker for breast cancer progression. However, the role of CCAT1 in regulating radiosensitivity of breast cancer and its underlying mechanism have not been investigated. The present study showed that CCAT1 was up-regulated and miR-148b was down-regulated in radioresistant breast cancer tissues compared with radiosensitive breast cancer tissues. Read More

    CacyBP/SIP inhibits the migration and invasion behaviors of glioblastoma cells through activating Siah1 mediated ubiquitination and degradation of cytoplasmic p27.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    School of Medicine, Shandong University, Jinan, 250012, China.
    Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP) has been reported to be up-regulated and plays an important role in promoting cell proliferation in human glioma. However, the effect of CacyBP/SIP on glioma cell motility is still unclear. Here, to our surprise, CacyBP/SIP was found to inhibit the migration and invasion of glioma cells U251 and U87. Read More

    Structural and functional features of lysine acetylation of plant and animal tubulins.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    Laboratory of Structural Biology, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, Ukraine.
    The study of the genome and the proteome of different species and representatives of distinct kingdoms, especially detection of proteome via wide-scaled analyses has various challenges and pitfalls. Attempts to combine all available information together and isolate some common features for determination of the pathway and their mechanism of action generally have a highly complicated nature. However, microtubule (MT) monomers are highly conserved protein structures, and microtubules are structurally conserved from Homo sapiens to Arabidopsis thaliana. Read More

    Intron length polymorphism of β-tubulin genes of Aegilops biuncialis Vis.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    Institute of Food Biotechnology and Genomics, Osipovskogo St., 2a, Kyiv-123, 04123, Ukraine.
    Intron-specific DNA polymorphism is present among plant β-tubulin gene family members and is considered to be one of the molecular markers based on the difference of tubulin introns length assayed both separately (TBP: 1st intron) or in combination (h-TBP: 1st and 2nd introns). These two approaches are possibly useful for wheat breeding programs, since TBP and h-TBP help to differentiate between the accessions of Aegilops biuncialis Vis., a wild relative of wheat. Read More

    CRISPR-Cas9 mediated gene knockout in human coronary artery endothelial cells reveals a pro-inflammatory role of TLR2.
    Cell Biol Int 2017 Oct 6. Epub 2017 Oct 6.
    Research Center for Vascular Biology, College of Medicine, Yangzhou University, Yangzhou, China.
    Endothelial inflammatory responses promote the development and progression of atherosclerosis. It was reported that Toll-like receptors 2 (TLR2) is associated with endothelial inflammation. However, the effect of TLR2 on inflammatory responses in human coronary artery endothelial cells (HCAECs) remains largely unknown. Read More

    Intermediate Filaments and Cellular Mechanics.
    Cell Biol Int 2017 Oct 5. Epub 2017 Oct 5.
    Department of Physics, Biophysics Group, Friedrich-Alexander-University Erlangen-Nuremberg, D-91052, Erlangen, Germany.
    Intermediate filaments (IFs) are one of the three types of cytoskeletal polymers that resist tensile and compressive forces in cells. They crosslink each other as well as with actin filaments and microtubules by proteins, which include desmin, filamin C, plectin, and lamin (A/C). Mutations in these proteins can lead to a wide range of pathologies, some of which exhibit mechanical failure of the skin, skeletal, or heart muscle. Read More

    Leptin differentially regulates endochondral ossification in tibial and vertebral epiphyseal plates.
    Cell Biol Int 2017 Oct 5. Epub 2017 Oct 5.
    Department of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, No. 160 Pujian Road, Shanghai, 200127, China.
    Longitudinal bone growth is governed by a complex network of endocrine signals including leptin. In mouse, leptin deficiency leads to distinct phenotypes in bones of the limb and spine, suggesting the appendicular and axial skeletons are subject to differential regulation by leptin. We established primary cultures for the chondrocytes from tibial and vertebral epiphyseal plates. Read More

    Cell Biol Int 2017 Oct 5. Epub 2017 Oct 5.
    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, Ukraine, Osipovskogo St. 2a, 04123.
    The presence of evolutionarily conserved NOS or NOS-like enzymes in land plants different than those in animals is still unclear, despite their activity has been revealed in cytosol and some organelles. At the same time, the emerging evidence for the importance of L-arginine-dependent pathways of NO synthesis in plant cells is still accumulating. The aim of our study was to reveal physiological effects on growth and differentiation processes and microtubular cytoskeleton organization of the competitive mammalian NO synthase inhibitor L-NAME. Read More

    Nintedanib treatment delays prostate dorsolateral lobe cancer progression in the TRAMP model: contribution to the epithelial-stromal interaction balance.
    Cell Biol Int 2017 Oct 5. Epub 2017 Oct 5.
    Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-865, São Paulo, Brazil.
    Prostate cancer (PCa) progression mechanism has been linked to epithelial proliferation, tumor invasion ability, and growth factors. Nintedanib (BIBF 1120) has been reported as being FGF and VEGF pathway inhibitors, exhibiting antitumor activity. Thus, the objective herein was to characterize the early Nintedanib treatment effects on the structure and molecules involved in the basal membrane, the extracellular matrix (ECM) maintenance, in addition to the angiogenesis and mitogenic processes at different grades of prostatic tumor development in TRAMP mice. Read More

    GPR120 is not required for ω-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells.
    Cell Biol Int 2017 Oct 5. Epub 2017 Oct 5.
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
    Intake of ω-3 PUFAs reduces the frequency of breast cancer, and GPR120 receptor transduces ω-3 PUFAs signaling to increase insulin sensitivity in mice, but whether GPR120 mediates ω-3 PUFAs signaling to inhibit breast carcinogenesis is currently unknown. In the present study, we found that GPR120 is highly expressed in human breast cancerous tissues but not adjacent normal tissue. Knockdown of GPR120 by siRNA in breast cancer cells significantly reduced cell growth, and dramatically increased ω-3 FFA-induced cell growth inhibition and apoptosis. Read More

    Involvement of RPL11 in the enhancement of P53 stability by a podophyllum derivative, a topoisomerase II inhibitor.
    Cell Biol Int 2017 Sep 26. Epub 2017 Sep 26.
    School of Public Health, Nanchang University, 461 Ba Yi Avenue, Nanchang, Jiangxi 330006, P. R. China.
    In previous work, we presented experimental and theoretical evidence that D-3F or 4-N-(2-Amino-3-fluoropyridine)-4-deoxidation-4'-demethylepipofophyllotoxin induced G2 /M phase arrest and apoptosis, purportedly by increasing the expression of P53. However, the precise mechanism of D-3F action is currently unknown. Here, we investigated the mechanism by which D-3F treatment induces increased expression of P53. Read More

    Cervical cancer stem-like cells: systematic review and identification of reference genes for gene expression.
    Cell Biol Int 2017 Sep 26. Epub 2017 Sep 26.
    Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre 90050-170, Rio Grande do Sul, Brazil.
    Cervical cancer is the fourth most common cancer affecting women worldwide. Among many factors, the presence of cancer stem cells, a subpopulation of cells inside the tumor, has been associated with a worse prognosis. Considering the importance of gene expression studies to understand the biology of cervical cancer stem cells (CCSC), this work identifies stable reference genes for cervical cancer cell lines SiHa, HeLa, and ME180 as well as their respective cancer stem-like cells. Read More

    Sprouty2 is involved in the control of osteoblast proliferation and differentiation through the FGF and BMP signaling pathways.
    Cell Biol Int 2017 Sep 16. Epub 2017 Sep 16.
    Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
    Fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) play essential roles in bone formation and osteoblast activity through the extracellular signal-regulated kinase 1/2 (ERK1/2) and Smad pathways. Sprouty family members are intracellular inhibitors of the FGF signaling pathway, and four orthologs of Sprouty have been identified in mammals. In vivo analyses have revealed that Sprouty2 is associated with bone formation. Read More

    A role for the non-receptor tyrosine kinase ACK1 in TNF-alpha-mediated apoptosis and proliferation in human intestinal epithelial caco-2 cells.
    Cell Biol Int 2017 Sep 16. Epub 2017 Sep 16.
    Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
    The roles of tumor necrosis factor alpha (TNF-alpha) and its mediators in cellular processes related to intestinal diseases remain elusive. In this study, we aimed to determine the biological role of activated Cdc42-associated kinase 1 (ACK1) in TNF-alpha-mediated apoptosis and proliferation in Caco-2 cells. ACK1 expression was knocked down using ACK1-specific siRNAs, and ACK1 activity was disrupted using a small molecule ACK1 inhibitor. Read More

    The toxic alpha-gliadin peptide 31-43 enters cells without a surface membrane receptor.
    Cell Biol Int 2017 Sep 15. Epub 2017 Sep 15.
    Chemistry and Biology, University of Salerno, Fisciano, Italy.
    Alpha-gliadin peptide 31-43 is considered to be the main peptide responsible for the innate immune response in celiac disease patients. Recent evidence indicates that peptide 31-43 rapidly enters cells and interacts with the early endocytic vesicular compartment. However, the mechanism of its uptake is not completely understood. Read More

    β-carotene suppresses Porphyromonas gingivalis lipopolysaccharide-mediated cytokine production in THP-1 monocytes cultured with high glucose condition.
    Cell Biol Int 2017 Sep 14. Epub 2017 Sep 14.
    Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima, 770-8504, Japan.
    Periodontitis is associated with development of diabetes mellitus. Although lipopolysaccharide (LPS) of Porphyromonas gingivalis (Pg), a major pathogen of periodontitis, may lead the progression of diabetes complications, the precise mechanisms are unclear. We, therefore, investigated the effects of β-carotene on production of Pg LPS-induced inflammatory cytokines in human monocytes cultured high glucose (HG) condition. Read More

    Collagen peptides modulate the metabolism of extracellular matrix by human dermal fibroblasts derived from sun-protected and sun-exposed body sites.
    Cell Biol Int 2017 Sep 14. Epub 2017 Sep 14.
    Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1524, Sao Paulo, SP, 05508-000, Brazil.
    Clinical data published in recent years have demonstrated positive effects of collagen hydrolysate (CH) on skin aging clinical signs. CH use as food supplement has a long history; however, few studies have addressed the underlying purpose of CH on the cellular and molecular biology of skin cells that could elucidate clinical improvement findings. Wide diversity of characteristics has been reported for dermal fibroblasts derived from different body sites and it is unknown whether collagen peptides could modulate differently cells from chronological aged and photoaged skin areas. Read More

    CDP-choline circumvents mercury-induced mitochondrial damage and renal dysfunction.
    Cell Biol Int 2017 Sep 8. Epub 2017 Sep 8.
    Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, México, D. F. México.
    Heavy metal ions are known to produce harmful alterations on kidney function. Specifically, the accumulation of Hg(2+) in kidney tissue may induce renal failure. In this work, the protective effect of CDP-choline against the deleterious effects induced by Hg(2+) on renal function was studied. Read More

    miRNA-4317 suppresses human gastric cancer cell proliferation by targeting ZNF322.
    Cell Biol Int 2017 Sep 7. Epub 2017 Sep 7.
    Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
    Studies have shown that miR-4317 is dysregulated in tumor, but the biologic role of miR-4317 in tumor development and progression remains unknown. The present study aimed to investigate the role of miR-4317 in human gastric cancer. Quantitative real-time PCR was used to quantify miR-4317 expression levels in clinical gastric cancer specimens and cell lines. Read More

    Induction of autophagy by an oleanolic acid derivative, SZC017, promotes ROS-dependent apoptosis through Akt and JAK2/STAT3 signaling pathway in human lung cancer cells.
    Cell Biol Int 2017 Sep 7. Epub 2017 Sep 7.
    Department of Pharmacology, Dalian Medical University, 9 West Section, South Road of Lvshun, Dalian, China.
    The signal transducers and activators of transcription 3 (STAT3) signaling pathway is a common feature in many solid tumors including non-small cell lung cancer, whereas current therapies usually fail to treat this disease in majority of cases. In the present study, we aimed to investigate the cytotoxic effect and the underlying mechanisms of SZC017, an oleanolic acid derivative, on human lung cancer cells. Cell viability was significantly decreased in SZC017-treated lung cancer cells. Read More

    Exosomes secreted from mutant-HIF-1α-modified bone-marrow-derived mesenchymal stem cells attenuate early steroid-induced avascular necrosis of femoral head in rabbit.
    Cell Biol Int 2017 Sep 6. Epub 2017 Sep 6.
    The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121001, P.R. China.
    Mesenchymal stem cells (MSCs)-derived exosomes exhibit protective effects on damaged or diseased tissues. Hypoxia-inducible factor 1α (HIF-1α) plays a critical role in bone development. However, HIF-1α is easily biodegradable under normoxic conditions. Read More

    Desquamation takes center stage at the origin of proliferative inflammatory atrophy, epithelial-mesenchymal transition, and stromal growth in benign prostate hyperplasia.
    Cell Biol Int 2017 Nov 29;41(11):1265-1270. Epub 2017 Sep 29.
    Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas SP, Brazil.
    In this commentary, we propose a relationship between desquamation, initially described as the collective detachment and deletion of epithelial cell in the prostate gland after castration, and proliferative inflammatory atrophy (PIA) and stromal growth in benign prostate hyperplasia (BPH). First, in response to diverse stimuli, including inflammatory mediators, epithelial cells desquamate and leave a large surface of the luminal side of the basement membrane (BM) exposed. Basal cells are activated into intermediate-type cells, which change morphology to cover and remodel the exposed BM (simple atrophy) to a new physiological demand (such as in the hypoandrogen environment, simulated by surgical and/or chemical castration) and/or to support re-epithelialization (under normal androgen levels). Read More

    SIRT1 mediates salidroside-elicited protective effects against MPP(+) -induced apoptosis and oxidative stress in SH-SY5Y cells: involvement in suppressing MAPK pathways.
    Cell Biol Int 2017 Aug 29. Epub 2017 Aug 29.
    Department of Neurology, Tianjin Medical University General Hospital, Tianjin 300052, China.
    Parkinson's disease (PD) is a progressive neurodegenerative disease, leading to tremor, rigidity, bradykinesia, and gait impairment. Salidroside has been reported to exhibit antioxidative and neuroprotective properties in PD. However, the underlying neuroprotective mechanisms effects of salidroside are poorly understood. Read More

    Macrophage migration inhibitory factor (MIF) promotes rat airway muscle cell proliferation and migration mediated by ERK1/2 and FAK signaling.
    Cell Biol Int 2017 Aug 29. Epub 2017 Aug 29.
    Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou North Avenue, Guangzhou 510515, China.
    Macrophage migration inhibitory factor (MIF) is an inflammatory mediator that contributes to asthmatic airway remodeling; however, little is known regarding the effects of MIF on airway smooth muscle cells (ASMCs). In the present study, we found that an enhanced expression of MIF promoted ASMC proliferation, increased the population of cells in the S/G2 phase, downregulated P21 expression, and upregulated cyclin D1, cyclin D3, and Cdk6 expression. In addition, the apoptosis of ASMCs was significantly decreased in response to MIF overexpression, compared with the negative control. Read More

    4-Phenybutyric acid promotes gastric cancer cell migration via histone deacetylase inhibition-mediated HER3/HER4 up-regulation.
    Cell Biol Int 2017 Aug 29. Epub 2017 Aug 29.
    Department of Medical Oncology, the First Hospital of China Medical University, Shenyang, 110001, China.
    Dysregulation of histone acetylation plays an important role in tumor development. Histone acetylation regulates gene transcription and expression, which is reversibly regulated by histone acetyltransferase (HAT) and histone deacetylase (HDAC). As an HDAC inhibitor, 4-phenylbutyric acid (4-PBA) can increase histone acetylation levels by inhibiting HDAC activity. Read More

    Wnt5a regulates the cell proliferation and adipogenesis via MAPK-independent pathway in early stage of obesity.
    Cell Biol Int 2017 Aug 29. Epub 2017 Aug 29.
    State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P.R. China.
    The early stage of obesity is an important stage in the development of obesity. However, there are few studies which explored the property or changes in obesity at early stage especially involving Wnt5a. The associated gene expression of Wnt5a on cell regeneration and the effect of Wnt5a on rat adipose-derived stem cell (rASC) proliferation and adipogenesis need additional study. Read More

    Comparative study of hematopoietic stem and progenitor cells between sexes in mice under physiological conditions along time.
    Cell Biol Int 2017 Aug 29. Epub 2017 Aug 29.
    LAGENBIO, Veterinary Faculty of Zaragoza, Instituto Agroalimentario de Aragón (IA2), IIS Aragón, University of Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain.
    Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs) frequencies by flow cytometry, by performing serial peripheral blood extractions from male and female B6SJL wild-type mice and found no significant differences. Only modest differences were found in the gene expression profile of Slamf1 and Gata2. Read More

    Simultaneous targeted inhibition of Sox2-Oct4 transcription factors using decoy oligodeoxynucleotides to repress stemness properties in mouse embryonic stem cells.
    Cell Biol Int 2017 Aug 18. Epub 2017 Aug 18.
    Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.
    Transcriptional master regulators like Sox2 and Oct4, which are expressed in various human tumors, have been shown to cause tumor growth promotion as well as epithelial dysplasia by means of interfering with progenitor cell differentiation. In order to investigate the potential of Sox2-Oct4 transcription factor decoy (TFD) strategy for differentiation therapy, mouse embryonic stem cells (mESCs) were used in this study as a model of cancer stem cells (CSCs). Sox2-Oct4 complex decoy ODNs (cd-ODNs) were designed according to their elements in the promoter region of Sox2 gene. Read More

    A novel model to characterize structure and function of BRCA1.
    Cell Biol Int 2017 Aug 18. Epub 2017 Aug 18.
    Isar Klinikum, Sonnenstr 24-26, Munich, 80331, Germany.
    BRCA1 plays a central role in DNA repair. Although N-terminal RING and C-terminal BRCT domains are studied well, the functions of the central region of BRCA1 are poorly characterized. Here, we report a structural and functional analysis of BRCA1 alleles and functional human BRCA1 in chicken B-lymphocyte cell line DT40. Read More

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