3,780 results match your criteria Cell Biology International [Journal]


Role of allograft inflammatory factor-1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, People's Republic of China.

Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated with inflammation in the cells other than macrophages, such as endothelial cells and vascular smooth muscle cells. To clarify the molecular mechanisms of AIF-1-modulated pathological changes in the peritoneum during PD, we first detected the AIF-1 expression in peritoneal tissues from PD mice. Read More

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http://dx.doi.org/10.1002/cbin.11115DOI Listing
February 2019

Expression of proteins related to thyroid hormone function in the uterus is down-regulated at the day of implantation in hypothyroid pregnant rats.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Department of Obstetrics & Gynecology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Hypothyroidism has been linked to infertility, but the mechanisms underlying infertility-related hypothyroidism have yet to be fully elucidated. Therefore, in this study, effects of hypothyroidism on expression of the proteins related to thyroid hormone function in the uterus, which were thought to play a role implantation, including thyroid hormone receptor (TR), thyroid stimulating hormone receptor (TSHR), retinoic acid receptor (RAR) and extracellular kinase (ERK) were identified. Pregnant female rats were rendered hypothyroid by giving methimazole (MMI), orally. Read More

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http://dx.doi.org/10.1002/cbin.11114DOI Listing
February 2019

Periostin plays role in force-induced stem cell potential by periodontal ligament stem cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Associate Professor, Orthodontic section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.

Mechanical stimuli have been shown to play an important role in directing stem cell fate and maintenance of tissue homeostasis. One of the functions of the mechanoresponsive tissue periodontal ligament (PDL) is to withstand the functional forces within the oral cavity. Periodontal ligament stem cells (PDLSCs) derived from periodontal tissue have been demonstrated to be able to respond directly to mechanical forces. Read More

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http://dx.doi.org/10.1002/cbin.11116DOI Listing
February 2019

Insulin-like growth factor-I promotes hepatic differentiation of human adipose tissue-derived stem cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

There are numerous studies which provide support for the use of human adipose tissue-derived stem cells (hASCs) to generate hepatocyte-like cells. However, the produced cells exhibit only a certain level of differentiation, mainly due to inefficient induction conditions. Therefore, based on the important role of insulin-like growth factor (IGF-I) in hepatic function and development, in the current study we evaluated the differentiation efficacy of the mentioned factor to induce hASCs into functional hepatocyte-like cells. Read More

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http://dx.doi.org/10.1002/cbin.11113DOI Listing
February 2019

The use of apocynin inhibits osteoclastogenesis.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Institute of Biomedical Science, Federal University of Uberlandia.

Reactive oxygen species (ROS) are produced by NADPH oxidase (NOX), an enzyme that reduces oxygen by using NADPH as a substrate. Apocynin (APO) is a catechol that is used as a NOX inhibitor, and N-acetyl-cysteine (NAC) can reduce intracellular ROS levels. In this work, the effect of APO and NAC on osteoclast formation were evaluated. Read More

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http://dx.doi.org/10.1002/cbin.11110DOI Listing
February 2019

Spinal cord tissue affects sprouting from aortic fragments in ex vivo co-culture.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

National Research Center Kurchatov Institute, Moscow, Russian Federation.

It is a well-known fact, that there is a close interconnection between vascular and neural structures in both embryonic development and postnatal life. Different models have been employed to dissect the mechanisms of these interactions, ranging from in vitro systems (e.g. Read More

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http://dx.doi.org/10.1002/cbin.11112DOI Listing
February 2019

HIV-1 infection modulates IL-24 expression which contributes to cell apoptosis IN VITRO.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Disciplina de Infectologia, Laboratório de Retrovirologia, Universidade Federal de São Paulo, São Paulo, Brazil.

Although interleukin-24 (IL-24) has been extensively explored in the immunopathologies of autoimmune diseases, neoplasms, and infections, its role in HIV-1 infection has not been thoroughly elucidated to date. Therefore, the objective of this study was to evaluate the gene and protein expressions of IL-24 at the initial moments of HIV infection in PBMCs. Due to the pro-apoptotic role of IL-24, we evaluated the protein expression of caspase-3, as well as Annexin V/ Propidium Iodide flow cytometry and phosphorylation of ERK, which may induce an apoptotic signal block when phosphorylated. Read More

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http://dx.doi.org/10.1002/cbin.11111DOI Listing
February 2019

THE DIFFERENT EXPRESSION OF KEY MARKERS ON UROTHELIAL HOLOCLONAL, MEROCLONAL AND PARACLONAL CELLS IN IN VITRO CULTURE.

Cell Biol Int 2019 Feb 6. Epub 2019 Feb 6.

Department of Regenerative Medicine, Cell and Tissue Bank, Chair of Urology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Poland.

Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative and self-renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Read More

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http://dx.doi.org/10.1002/cbin.11109DOI Listing
February 2019

IL-7 enhances the differentiation of adipose-derived stem cells toward lymphatic endothelial cells through AKT signaling.

Cell Biol Int 2019 Jan 28. Epub 2019 Jan 28.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, P. R. China.

Our study was designed to investigate the effects of IL-7 during the differentiation process of adipose-derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs). IL-7 was added to the traditional induced medium, which was called the IL-7 (+) group, while the group that used traditional induced medium was called the IL-7 (-) group. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups. Read More

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http://dx.doi.org/10.1002/cbin.11093DOI Listing
January 2019
1 Read

Polypeptides secreted from the columnar vesicles of the sea anemone Bunodosoma cangicum and their in vivo effects on Caenorhabditis elegans.

Cell Biol Int 2019 Jan 23. Epub 2019 Jan 23.

Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, Rio Grande do Sul 96203-900, Brazil.

In this study we provide new evidence that the columnar vesicles of the sea anemone Bunodosoma cangicum are toxic in vivo and contain at least two active polypeptides, a neurotoxic and an apoptosis inducing polypeptide. Here we show that it is also an effective inducer of apoptosis in vivo in the nematode Caenorhabditis elegans. In addition, the anemone peptides rapidly paralyze C. Read More

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http://dx.doi.org/10.1002/cbin.11107DOI Listing
January 2019
3 Reads

Improvements in chemical carriers of proteins and peptides.

Authors:
Azam Bolhassani

Cell Biol Int 2019 Jan 23. Epub 2019 Jan 23.

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

The successful intracellular delivery of biologically active proteins and peptides plays an important role for therapeutic applications. Indeed, protein/ peptide delivery could overcome some problems of gene therapy, e.g. Read More

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http://dx.doi.org/10.1002/cbin.11108DOI Listing
January 2019
2 Reads

Silencing of MEG3 inhibited ox-LDL-induced inflammation and apoptosis in macrophages via modulation of the MEG3/miR-204/CDKN2A regulatory axis.

Cell Biol Int 2019 Jan 23. Epub 2019 Jan 23.

The First Bethune Hospital of Jilin University, 71 Xinmin Street, Chaoyang District, Changchun City, 130012, Jilin Province, People's Republic of China.

Atherosclerosis (AS) is one of the most common chronic inflammatory diseases and a major cause of morbidity and mortality. However, the underlying molecular mechanisms of the progression of AS are still largely unknown. Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) play important roles in a variety of biological processes and the physiological and pathological progression of human diseases. Read More

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http://dx.doi.org/10.1002/cbin.11105DOI Listing
January 2019
1 Read

Functional prediction and characterization of Dip2 gene in mice.

Cell Biol Int 2019 Jan 23. Epub 2019 Jan 23.

Key Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130021, China.

Disconnected interacting protein 2 (DIP2) is a highly conserved protein family among invertebrates and vertebrates, but its function remains unclear. In this paper, we summarized the conservation of gene sequences and protein domains of DIP2 family members and predicted that they may have a similar functional role in acetyl-coenzyme A (acetyl-CoA) synthesis. We then used the most characterized member, disconnected interacting protein 2 homolog A (DIP2A), for further study. Read More

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http://dx.doi.org/10.1002/cbin.11106DOI Listing
January 2019
4 Reads

MiR-9 promotes multiple myeloma progression by regulating TRIM56/NF-κB pathway.

Cell Biol Int 2019 Jan 14. Epub 2019 Jan 14.

Department of Hematology, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, 710061, China.

MiR-9 has been reported to play a pivotal role in multiple human cancers through acting as an oncogene or tumor suppressor. In this study, we explored the possible role and molecular mechanism of miR-9 in multiple myeloma (MM). MiR-9 expression was examined by qRT-PCR assay. Read More

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http://doi.wiley.com/10.1002/cbin.11104
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http://dx.doi.org/10.1002/cbin.11104DOI Listing
January 2019
5 Reads

Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus-affected gestations: A focus on myogenic and neural differentiations.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, Vilnius, LT-10257, Lithuania.

Amniotic fluid-derived mesenchymal stem cells (AF-MSCs) are autologous to the fetus and represent a potential alternative source for the regenerative medicine and treatment of perinatal disorders. To date, AF-MSCs differentiation capacity to non-mesodermal lineages and epigenetic regulation are still poorly characterized. The present study investigated the differentiation potential of AF-MSCs toward neural-like cells in comparison to the mesodermal myogenic lineage and assessed epigenetic factors involved in tissue-specific differentiation. Read More

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http://dx.doi.org/10.1002/cbin.11099DOI Listing
January 2019
1 Read

The Hippo-YAP Pathway Regulates the Proliferation of Alveolar Epithelial Progenitors after Acute Lung Injury.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Military Medical University, Chongqing 400042, China.

Regeneration of pulmonary epithelial cells plays an important role in the recovery of acute lung injury (ALI) which is defined by pulmonary epithelial cell death. However, the mechanism of the regenerative capacity of alveolar epithelial cells is unknown. Using a lung injury mouse model induced by hemorrhagic shock and LPS, a protein mass spectrometry-based high-throughput screening and linage tracing technology to mark alveolar epithelial type 2 cells (AEC2s), we analyzed the mechanism of alveolar epithelial cells proliferation. Read More

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http://doi.wiley.com/10.1002/cbin.11098
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http://dx.doi.org/10.1002/cbin.11098DOI Listing
January 2019
7 Reads

ER stress differentially affects pro-inflammatory changes induced by mitochondrial dysfunction in the human monocytic leukemia cell line, THP-1.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, Republic of Korea.

The functional and physical interaction between mitochondria and the endoplasmic reticulum (ER) has been the subject of intense study. To test the effect of this interaction on macrophage inflammatory activation, the human macrophage-like monocytic leukemia cell line THP-1 was treated with oligomycin, rotenone, or sodium azide, which induce mitochondrial dysfunction (MD) by blocking the electron transport chain (ETC). MD induced by these agents triggered activation of various sensors and markers of ER stress. Read More

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http://doi.wiley.com/10.1002/cbin.11103
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http://dx.doi.org/10.1002/cbin.11103DOI Listing
January 2019
3 Reads

GEN-27 exhibits anti-inflammatory effects by suppressing the activation of NLRP3 inflammasome and NF-κB pathway.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

Clinical Laboratory, Xi 'an No.4 Hospital, 710004, China.

Prolonged inflammation and deregulated cytokine production are associated with diversified inflammatory diseases. Genistein (GEN), the active and predominant isoflavonoid in dietary soybean, possesses anti-inflammatory activity. Our study aimed to assess the anti-inflammatory effects of GEN-27, a derivative of GEN, as well as explore the potential molecular mechanisms using lipopolysaccharide (LPS)-induced RAW264. Read More

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http://doi.wiley.com/10.1002/cbin.11101
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http://dx.doi.org/10.1002/cbin.11101DOI Listing
January 2019
7 Reads

IL-1β functionally attenuates ABCG2 and PDZK1 expression in HK-2 cells partially through NF-ĸB activation.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

Department of Rheumatology, the Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, 310009, China.

Long-standing untreated hyperuricemia could lead to gout. Several recent studies have demonstrated a significant decrease of serum urate during acute gout attack, which is an aseptic inflammation process focusing on IL-1β. However, how IL-1β, by itself, alters the expression and the functional activity of urate transporters in renal tubular epithelial cells is still unclear. Read More

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http://doi.wiley.com/10.1002/cbin.11100
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http://dx.doi.org/10.1002/cbin.11100DOI Listing
January 2019
8 Reads
1.933 Impact Factor

Calycosin stimulates the osteogenic differentiation of rat calvarial osteoblasts by activating the IGF1R/PI3K/Akt signaling pathway.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA, Lanzhou, 730050, Gansu Province, People's Republic of China.

Calycosin has been reported to have a strong osteogenic activity and a positive correlation with anti-osteoporosis effects. However, its precise mechanism of action remains unclear. Since insulin-like growth factor 1 receptor (IGF1R) signaling and phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signaling have been shown to play a pivotal role in regulating osteogenesis, we hypothesized that the osteogenic activity of calycosin is mediated by these signaling pathways. Read More

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http://doi.wiley.com/10.1002/cbin.11102
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http://dx.doi.org/10.1002/cbin.11102DOI Listing
January 2019
6 Reads

MNX1 promotes cell proliferation and activates Wnt/β-catenin signaling in colorectal cancer.

Cell Biol Int 2019 Jan 7. Epub 2019 Jan 7.

Department of general surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou 215002, Jiangsu, China.

Aberrant Wnt/β-catenin signaling is a characteristic feature of colorectal cancer (CRC), therefore, understanding the underlying mechanisms of aberrant Wnt/β-catenin signaling will improve the treatment outcome of CRC. Expression of MNX1 in paired fresh CRC tissues and corresponding adjacent normal tissues were examined by qPCR and western blotting. The levels of MNX1 in paraffin-embedded CRC specimens were detected by immunohistochemistry (IHC). Read More

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http://doi.wiley.com/10.1002/cbin.11096
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http://dx.doi.org/10.1002/cbin.11096DOI Listing
January 2019
11 Reads

Mitsugumin 53 promotes mitochondrial autophagy through regulating Ambra1 expression in C2C12 myoblast cells.

Cell Biol Int 2019 Jan 7. Epub 2019 Jan 7.

Department of Nephrology, Shanghai General Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200080, China.

In this study, we investigated the function of Mitsugumin 53 (MG53) in regulation of mitochondrial autophagy in skeletal muscle cells and explored its potential application in the prevention and treatment of skeletal muscle atrophy in rats with chronic kidney disease (CKD). The expression of autophagy beclin 1 regulator 1 (Ambra1) and MG53 in skeletal muscles of 5/6 nephrectomized rats was measured, and the effect of MG53 on mitochondrial autophagy of C2C12 myoblasts was investigated by in vitro experiments. Our results show the expression of Ambra1 and MG53 in the skeletal muscle of CKD rats was significantly decreased. Read More

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http://dx.doi.org/10.1002/cbin.11097DOI Listing
January 2019
1 Read

LncRNA MAFG-AS1 facilitates the migration and invasion of NSCLC cell via sponging miR-339-5p from MMP15.

Cell Biol Int 2019 Jan 1. Epub 2019 Jan 1.

Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy, Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, China.

Non-small-cell carcinoma (NSCLC) is the most common cancer along with high mortality rate worldwide. In the present study, our data showed that lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAFG-AS1) was over-expressed in NSCLC tissues and cell lines. Overexpression of MAFG-AS1 promoted the migration, invasion and enhanced epithelial-mesenchymal transition (EMT) of NSCLC cell. Read More

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http://dx.doi.org/10.1002/cbin.11092DOI Listing
January 2019
2 Reads

Multidrug resistance phenotype: Relation between phenotype induction and its characteristics in erythroleukemia cells.

Cell Biol Int 2019 Feb;43(2):214-219

Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande- FURG, Av. Itália, Km 8, CEP 96203-900 Rio Grande, RS, Brasil.

Chemotherapy may be followed by multiple drug resistance (MDR). This is an obstacle in the treatment of cancer. It is therefore essential to understand the mechanisms underlying tumor resistance, especially those involved in the cell target/MDR relationship. Read More

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http://dx.doi.org/10.1002/cbin.11095DOI Listing
February 2019
2 Reads

Synergistic interaction of Deltex and Hrp48 leads to JNK activation.

Cell Biol Int 2018 Dec 31. Epub 2018 Dec 31.

Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.

The communication among the cells plays a seminal role in metazoan development by coordinating multiple cellular processes that, in turn, helps in the maintenance of biological homeostasis. Our previous study demonstrated that Dx and Hrp48 together downregulate Notch signaling and induce cell death in Drosophila. To understand the signaling events behind the Dx and Hrp48-induced cell death in a greater detail, we performed a set of genetic experiments followed by immunocytochemical analyses. Read More

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http://dx.doi.org/10.1002/cbin.11089DOI Listing
December 2018
2 Reads
1.933 Impact Factor

Smart hydrogels with high tunability of stiffness as a biomimetic cell carrier.

Cell Biol Int 2019 Feb;43(2):84-97

Hubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Department of Mechanics and Engineering Structure, Wuhan University of Technology, Wuhan, China.

Human tissues are sophisticated ensembles of various distinct cell types encapsulated in the biomechanical cues of the extracellular matrix. It has been known matrix stiffness plays a pivot role in cellular events and tissue-scale biological processes. Thus, materials that can mimic mechanical environments of tissues in vitro and possess wide, physiologically relevant elasticity are highly desirable. Read More

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http://dx.doi.org/10.1002/cbin.11091DOI Listing
February 2019
2 Reads

Three-dimensional nanofibrous polystyrene scaffolds modify macrophage phenotypes and activate macrophage angiogenic potential.

Cell Biol Int 2018 Dec 31. Epub 2018 Dec 31.

Department of Pathology, Center for Biostructure, Medical University of Warsaw, Chałubińskiego 5, Warsaw 02-004, Poland.

3D scaffolds represent an attractive substrate for studying macrophage activation and modification since they mimic extracellular matrix (ECM). However, macrophage response to such materials, particularly with respect to angiogenic potential is still poorly recognized. Therefore, we investigated the effect of 3D nanofibrous polystyrene scaffolds (NPSs) versus tissue culture polystyrene (TCPS) on THP-1-derived macrophages in various environmental conditions, for example, standard (m0), pro-inflammatory (m1), or anti-inflammatory (m2) with respect to pro-angiogenic potential. Read More

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http://dx.doi.org/10.1002/cbin.11094DOI Listing
December 2018
2 Reads

Activation of NF-κB signaling pathway during HCG-induced VEGF expression in luteal cells.

Cell Biol Int 2018 Dec 31. Epub 2018 Dec 31.

Provincial Key Laboratory for Developmental Biology and Neurosciences, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, No. 8, Shangsan Road, Fuzhou 350007, P. R. China.

Vascular endothelial growth factor (VEGF) plays an essential role in luteal angiogenesis, the present study therefore utilized luteal cells cultured in vitro to further investigate the activation and contribution of nuclear factor (NF)-κB to VEGF expression induced by human chorionic gonadotrophin (HCG). The present results showed HCG induced VEGF expression as well as hypoxia-inducible factor (HIF)-1α mRNA and protein expressions, which was blocked by NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC). Further analysis found that these increases of VEGF and HIF-1α mRNA induced by HCG were also blocked by NF-κB siRNA transfection, which was consistent with PDTC treatment. Read More

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http://dx.doi.org/10.1002/cbin.11090DOI Listing
December 2018
2 Reads

IL-6 stimulation of DNA replication is JAK1/2 mediated in cross-talk with hyperactivated ERK1/2 signaling.

Cell Biol Int 2019 Feb 7;43(2):192-206. Epub 2019 Jan 7.

Department of Molecular Oncology, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.

Myeloproliferative neoplasms (MPNs) are developing resistance to therapy by JAK1/2 inhibitor ruxolitinib. To explore the mechanism of ruxolitinib's limited effect, we examined the JAK1/2 mediated induction of proliferation related ERK1/2 and AKT signaling by proinflammatory interleukin-6 (IL-6) in MPN granulocytes and JAK2V617F mutated human erythroleukemia (HEL) cells. We found that JAK1/2 or JAK2 inhibition prevented the IL-6 activation of STAT3 and AKT pathways in polycythemia vera and HEL cells. Read More

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http://dx.doi.org/10.1002/cbin.11084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347554PMC
February 2019
3 Reads
1.933 Impact Factor

Ozone alleviates ischemia-reperfusion injury by inhibiting mitochondrion-mediated apoptosis pathway in SH-SY5Y cells.

Cell Biol Int 2018 Dec 20. Epub 2018 Dec 20.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha 410005, P.R.China.

Cerebral ischemia reperfusion (I/R) injury is commonly observed, and often causes severe complications. Ozone has been applied for protecting ischemia/reperfusion (I/R) injury in animal models of several organs including cerebra, but the detailed mechanism remains unclear. MTT assay and LDH measurement were used to determine the influence of ozone on cell activity and damage of SH-SY5Y cells. Read More

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http://dx.doi.org/10.1002/cbin.11087DOI Listing
December 2018
2 Reads

RXXPEG motif of MERIT40 is required to maintain spindle structure and function through its interaction with Tankyrase1.

Cell Biol Int 2019 Feb;43(2):174-181

Institute for Translational Medicine, Qingdao University, Qingdao, 266071, China.

Deubiquitinase BRISC complex plays important role in the maintenance of spindle structure and function; however, the underlying mechanism remains largely undefined. Here we demonstrated that MERIT40, a core component of BRISC complex, directly interacts with the RXXPEG motif in the ARC-V domain of Tankyrase1(TNKS1). Mutation of the RXXPEG motif in the MERIT40 (R28A) disrupted its interaction with TNKS1. Read More

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http://dx.doi.org/10.1002/cbin.11086DOI Listing
February 2019
1 Read
1.933 Impact Factor

RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice.

Cell Biol Int 2018 Dec 20. Epub 2018 Dec 20.

Department of Orthopedics, Shandong Energy Zibo Mining Group Co. Ltd. Central Hospital, 133 Zikuang Road, Zichuan District, Zibo 255120, Shandong, P.R China.

Cardiac fibrosis is associated with various cardiovascular diseases and can eventually lead to heart failure. Dysregulation of long non-coding RNAs (lncRNAs) are recognized as one of the key mechanisms of cardiac diseases. However, the roles and underlying mechanisms of lncRNAs in cardiac fibrosis have not been explicitly defined. Read More

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http://doi.wiley.com/10.1002/cbin.11085
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http://dx.doi.org/10.1002/cbin.11085DOI Listing
December 2018
7 Reads

miRNA-126 enhances viability, colony formation, and migration of keratinocytes HaCaT cells by regulating PI3 K/AKT signaling pathway.

Cell Biol Int 2019 Feb 11;43(2):182-191. Epub 2019 Jan 11.

Department of Dermatology, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, 264000, China.

Wound healing is a basic biological process including proliferation and migration of keratinocyte. The effects of microRNAs on skin wound healing remain largely unexplored. This study aimed to investigate the role of microRNA-126 (miR-126) in human skin wound healing. Read More

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http://dx.doi.org/10.1002/cbin.11088DOI Listing
February 2019
3 Reads

MicroRNA-1204 promotes cell proliferation by regulating PITX1 in non-small-cell lung cancer.

Cell Biol Int 2018 Dec 14. Epub 2018 Dec 14.

Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

MicroRNA-1204 (miR-1204), a member of the PVT1 region, may improve B cell differentiation and metastasis in breast cancer. However, the role of miR-1204 in non-small-cell lung cancer (NSCLC) and its mechanism remain unclear. The GEO public database was first employed to find differentially expressed genes. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11083
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http://dx.doi.org/10.1002/cbin.11083DOI Listing
December 2018
7 Reads

Quantitative analysis on secretion level of CDNF regulated by two key α-helices in CDNF protein.

Cell Biol Int 2019 Feb 8;43(2):167-173. Epub 2019 Jan 8.

Yuhuangding Hospital, Yantai, Shandong Province, China.

Cerebral dopamine neurotrophic factor (CDNF) has been considered as potent candidates for the therapy of Parkinson's disease (PD) for which can promote the survival of midbrain dopaminergic neurons. In addition to secret out from cells like other classical neurotrophic factors (NTFs), CDNF can locate in the endoplasmatic reticulum (ER), where they can function as ER stress response protein to regulate ER stress. In our previous studies, we have found two helices, α1 and α7, which can regulate the intracellular trafficking and secretion of CDNF. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11082
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http://dx.doi.org/10.1002/cbin.11082DOI Listing
February 2019
6 Reads

Circular RNA-0007874 (circMTO1) reverses chemoresistance to temozolomide by acting as a sponge of microRNA-630 in glioblastoma.

Cell Biol Int 2018 Dec 11. Epub 2018 Dec 11.

Department of Rehabilitation Medicine, Nanjing Brain Hospital Affiliated with Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

Temozolomide (TMZ) is typically used to treat glioblastoma; however, its effects are very limited because of the chemoresistance of glioblastoma. The mechanism of the chemoresistance of glioblastoma to TMZ remains largely unclear. In the present study, we found that circular RNA MTO1 (circMTO1) expression was significantly downregulated in TMZ-resistant glioblastoma tissues and cells. Read More

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http://dx.doi.org/10.1002/cbin.11080DOI Listing
December 2018
3 Reads
1.933 Impact Factor

Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs.

Cell Biol Int 2019 Feb 8;43(2):158-166. Epub 2019 Jan 8.

Department of Cardiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, 264100, P. R. China.

Long noncoding RNAs (lncRNAs) have been found to play important roles in nearly all biological processes. However, the functions of the majority of LncRNAs are not fully clear. Here we evaluated the function of lncRNA OGFRP1, which has not been previously annotated, in human coronary artery endothelial cells (HCAECs). Read More

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http://dx.doi.org/10.1002/cbin.11081DOI Listing
February 2019
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Silencing astrocyte elevated gene-1 attenuates lipopolysaccharide-induced inflammation and mucosal barrier injury in NCM460 cells by suppressing the activation of NLRP3 inflammasome.

Cell Biol Int 2019 Jan;43(1):56-64

Department of General Surgery, Jingmen No. 1 People's Hospital, No. 168 Xiangshan Road, Jingmen, Hubei Province, 448000, China.

The effect of oncogene astrocyte-elevated gene-1 (AEG-1) on noncancerous colonic epithelial cell disease is rarely studied. We aim to investigate the role of AEG-1 in lipopolysaccharide (LPS)-induced inflammation and mucosal barrier injury, and their potential mechanisms. NCM460 cells were stimulated by different concentrations of LPS at 12 h or 24 h. Read More

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http://dx.doi.org/10.1002/cbin.11078DOI Listing
January 2019
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Silymarin ameliorates diabetic cardiomyopathy via inhibiting TGF-β1/Smad signaling.

Cell Biol Int 2019 Jan;43(1):65-72

Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150081, China.

Diabetic cardiomyopathy (DCM) is the leading cause of morbidity and mortality in diabetes mellitus (DM) patients. Previous studies have shown that the transforming growth factor-beta 1 (TGF-β1)/Smad signaling pathway plays a key role in the development of myocardial fibrosis in DCM. Silymarin (SMN) is used clinically to treat liver disorders and acts by influencing TGF-β1. Read More

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http://doi.wiley.com/10.1002/cbin.11079
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http://dx.doi.org/10.1002/cbin.11079DOI Listing
January 2019
7 Reads
1.933 Impact Factor

Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR-101-3p/WEE1 axis.

Cell Biol Int 2019 Jan;43(1):44-55

Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang 330006, Jiangxi Province, P. R. China.

Radioresistance is a major obstacle in hepatocellular carcinoma (HCC) radiotherapy. Aberrant expression of long non-coding RNA (lncRNA) has been postulated to be implicated in the development of HCC radioresistance. We investigated the role of lncRNA nuclear enriched abundant transcript 1_2 (NEAT1_2) in radioresistance of HCC and its molecular mechanism in this study. Read More

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http://dx.doi.org/10.1002/cbin.11077DOI Listing
January 2019
4 Reads

MicroRNA-1254 contributes to the controlling of pro-fibrogenic environment in LX-2 cells by modulating SMAD3 and wound repair: new insights in hepatic fibrosis.

Cell Biol Int 2018 Nov 23. Epub 2018 Nov 23.

Laboratório de Biologia Molecular, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista "Júlio de Mesquita Filho" - Campus Rio Claro, Rio Claro, SP, 13506-900, Brazil.

Hepatic fibrosis and its end-stage cirrhosis have increased worldwide, and, despite all the efforts, no successful therapy is available. More recently, the heptapeptide angiotensin-(1-7) [ang-(1-7)] was reported to be able to modulate liver fibrosis and even steatosis; however, the molecular bases of these effects are not clear. In this study, we investigated the overexpression of the microRNA-1254 in the human hepatic stellate cell line LX-2, based on the effect of the heptapeptide in such cells, previously, demonstrated by our research group. Read More

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http://dx.doi.org/10.1002/cbin.11075DOI Listing
November 2018
7 Reads

Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line.

Cell Biol Int 2019 Jan;43(1):73-82

Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil.

Osmotic alterations are associated with several human diseases, including diabetic nephropathy. We have previously shown that high glucose, which is a well-known osmotic agent, induces significant disruption of the tight junction (TJ)-mediated tubular barrier of the Madin-Darby canine kidney (MDCK) cell line. In this study, we investigated the effect of acute (24 h) and chronic (72 h) exposure to increased osmolality (with a 14. Read More

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http://dx.doi.org/10.1002/cbin.11074DOI Listing
January 2019
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Bufalin induces protective autophagy by Cbl-b regulating mTOR and ERK signaling pathways in gastric cancer cells.

Cell Biol Int 2019 Jan;43(1):33-43

Department of Medical Oncology, Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning Province, The First Hospital of China Medical University, No. 155, North Nanjing Street, Heping District, Shenyang, 110001, China.

Bufalin, a natural small-molecule compound derived from the traditional Chinese medicine Chan su, has shown promising anti-cancer effects against a broad variety of cancer cells through different mechanisms. It has been reported to induce autophagy in gastric cancer cells. However, the molecular mechanism involved is not fully elucidated. Read More

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http://dx.doi.org/10.1002/cbin.11076DOI Listing
January 2019
9 Reads

Retracted: Salem, A. M., Ahmed, H. H., Atta, H. M., Ghazy, M. A. and Aglan, H. A. (2014), Potential of bone marrow mesenchymal stem cells in management of Alzheimer's disease in female rats. Cell Biol Int, 38: 1367-1383. doi:10.1002/cbin.10331.

Authors:

Cell Biol Int 2018 Dec;42(12):1695

The above article, published online on 07 July 2014 in the Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor in Chief (Sergio Schenkman) and John Wiley & Sons Ltd. The retraction has been agreed due to the overlap of data, figures and tables published in the following paper: Ahmed HH, Shousha WG, El-Sayed Mahdy, Rashed LA, Abdo SM. Read More

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http://dx.doi.org/10.1002/cbin.11064DOI Listing
December 2018
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Phenotypic stability of the human MG-63 osteoblastic cell line at different passages.

Cell Biol Int 2019 Jan;43(1):22-32

Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.

One of the most popular cell lines in osteogenesis studies is the human osteoblastic line MG-63. For cell biological investigation, it is important that the cells remain stable in their phenotype over several passages in cell culture. MG-63 cells can be used to provide fundamental insights into cell--material interaction. Read More

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http://doi.wiley.com/10.1002/cbin.11073
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January 2019
9 Reads

miR-381 overcomes cisplatin resistance in breast cancer by targeting MDR1.

Cell Biol Int 2019 Jan;43(1):12-21

Department of General Surgery, Nanjing Drum Tower Hospital, Nanjing, Jiangsu Province, 210008, China.

Increasing evidence suggests the involvement of microRNA-381 (miR-381) in chemoresistance of cancer treatment. However, its function and molecular mechanisms in breast cancer chemoresistance are still not well elucidated. In the present study, we aimed to investigate the functional role of miR-381 in cisplatin (DDP) resistance of breast cancer and discover the underlying molecular mechanism. Read More

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http://doi.wiley.com/10.1002/cbin.11071
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http://dx.doi.org/10.1002/cbin.11071DOI Listing
January 2019
13 Reads

Human neonatal stem cell-derived skin substitute improves healing of severe burn wounds in a rat model.

Cell Biol Int 2019 Feb;43(2):147-157

National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.

Conventional approaches can repair minor skin injuries; however, severe burn injuries require innovative approaches for efficient and better wound repair. Recent studies indicate that stem cell-based regenerative therapies can restore severe damaged skin both structurally and functionally. The current study aims to evaluate the wound healing potential of skin substitute derived from human neonatal stem cells (hNSCs) using a severe burn injury rat model. Read More

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http://dx.doi.org/10.1002/cbin.11072DOI Listing
February 2019
3 Reads

Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro.

Cell Biol Int 2019 Feb;43(2):136-146

Department of Structural and Functional Biology, State University of Campinas (UNICAMP), São Paulo, Brazil.

Hyperbaric oxygen is a clinical treatment that contributes to wound healing by increasing fibroblasts proliferation, collagen synthesis, and production of growth factors, inducing angiogenesis and inhibiting antimicrobial activity. It also has been shown that hyperbaric oxygen treatment (HBO), through the activation of nitric oxide synthase promotes an increase in the nitric oxide levels that may improve endothelial progenitor cells (EPC) mobilization from bone marrow to the peripheral blood and stimulates the vessel healing process. However, cellular mechanisms involved in cell proliferation and activation of EPC after HBO treatment remain unknown. Read More

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http://doi.wiley.com/10.1002/cbin.11070
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http://dx.doi.org/10.1002/cbin.11070DOI Listing
February 2019
14 Reads

Human bone marrow-derived MSCs spontaneously express specific Schwann cell markers.

Cell Biol Int 2018 Oct 25. Epub 2018 Oct 25.

Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.

In peripheral nerve injuries, Schwann cells (SC) play pivotal roles in regenerating damaged nerve. However, the use of SC in clinical cell-based therapy is hampered due to its limited availability. In this study, we aim to evaluate the effectiveness of using an established induction protocol for human bone marrow derived-MSC (hBM-MSCs) transdifferentiation into a SC lineage. Read More

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http://doi.wiley.com/10.1002/cbin.11067
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http://dx.doi.org/10.1002/cbin.11067DOI Listing
October 2018
10 Reads

CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-κB.

Cell Biol Int 2018 Dec;42(12):1680-1687

Department of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, No. 20 Yuhuangding Dong Road, Yantai, 264000, Shandong, China.

Our study aimed to explore the function of cystic fibrosis transmembrane conductance regulator (CFTR) in esophageal cancer. Twenty patients with esophageal squamous cell carcinoma (ESCC) and 20 patients with esophageal adenocarcinoma (EA) were enrolled in this study. The levels of CFTR and NF-κB in tumor tissues and adjacent normal tissues were detected, respectively. Read More

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http://doi.wiley.com/10.1002/cbin.11069
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http://dx.doi.org/10.1002/cbin.11069DOI Listing
December 2018
4 Reads
1.933 Impact Factor