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    Selenocysteine inhibits human osteosarcoma cells growth through triggering mitochondrial dysfunction and ROS-mediated p53 phosphorylation.
    Cell Biol Int 2018 Jan 11. Epub 2018 Jan 11.
    Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China.
    Osteosarcoma represents the most common primary malignant bone tumor in children and adolescents, which shows severe resistance towards standard chemotherapy because of high invasive capacity and growing incidence. Selenocystine (SeC) is a naturally available Se-containing amino acid that displays splendid anticancer activities against several human tumors. However, little information about SeC-induced growth inhibition against human osteosarcoma is available. Read More

    A small population of resident limb bud mesenchymal cells expresses few MSC-associated markers, but the expression of these markers is increased immediately after cell culture.
    Cell Biol Int 2018 Jan 4. Epub 2018 Jan 4.
    Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México Ciudad Universitaria, Apartado Postal 70228, México.
    Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors or multipotent mesenchymal stromal cells (MSC). Derived-MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Read More

    Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures.
    Cell Biol Int 2017 Dec 30. Epub 2017 Dec 30.
    Department of Cell Biology and Biotechnology, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Osipovskogo St., 2a, Kyiv, 04123, Ukraine.
    Cytoskeleton is gaining the increasing recognition as one of nitric oxide (NO) -downstream targets because of its involvement in plenty of NO-controlled processes in plants throughout the entire life cycle starting from seed germination to pollination as well as (a)biotic stress tolerance. It has been revealed that low temperature (+0.5°C) has an inhibitory effect on A. Read More

    IL-1α inhibits proliferation and adipogenic differentiation of human adipose-derived mesenchymal stem cells through NF-κB- and ERK1/2- mediated proinflammatory cytokines.
    Cell Biol Int 2017 Dec 30. Epub 2017 Dec 30.
    Institute of Aging Research, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan 523808, Guangdong Province, China.
    Dysfunctional adipogenesis such as subcutaneous lipoatrophy is closely related to insulin resistance and metabolic disorders. Although the expression or release of the cytokine interleukin-1α(L-1α) is known to increase in adipose tissue in response to cell death, cell senescence, aging, or solar radiation, the regulatory role of IL-1α in adipogenesis has not been sufficiently investigated. To investigate the problem, we explored the effect of IL-1α on the proliferation and adipogenic differentiation of human adipose-derived mesenchymal stem cells (ADSCs) using cell counting, alamarBlue assay, oil O staining, Western blot, among others. Read More

    Knockdown of CPEB4 expression suppresses cell migration and invasion via Akt pathway in non-small cell lung cancer.
    Cell Biol Int 2017 Dec 29. Epub 2017 Dec 29.
    Department of Medical Oncology, First People's Hospital of Yunnan Province, No. 157 of Jinbi Road, Xishan district of Kunming City, Yunnan Province, P.R. China.
    Cytoplasmic polyadenylation element binding protein 4 (CPEB4) could be an important regulator in variety of cancers. However, the biological function and the underlying molecular mechanism of CPEB4 in non-small cell lung cancer (NSCLC) remains unknown. In this study, we investigated the metastasis role of CPEB4 in NSCLC cells, we knocked down CPEB4 using siRNA. Read More

    The mitochondrial calcium uniporter complex in trypanosomes.
    Cell Biol Int 2017 Dec 29. Epub 2017 Dec 29.
    Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
    The presence of a conserved mechanism for mitochondrial calcium uptake in trypanosomatids was crucial for the molecular identification of the mitochondrial calcium uniporter (MCU), a long-sought channel present in most eukaryotic organisms. Since then, research efforts to elucidate the role of MCU and its regulatory elements in different biological models have multiplied. MCU is the pore-forming subunit of a multimeric complex (the MCU complex or MCUC) and its predicted structure in trypanosomes is simpler than in mammalian cells, lacking two of its subunits and probably possessing other unidentified components. Read More

    Mitochondrial ATAD3A regulates milk biosynthesis and proliferation of mammary epithelial cells from dairy cow via the mTOR pathway.
    Cell Biol Int 2017 Dec 29. Epub 2017 Dec 29.
    The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China.
    ATPase family AAA-domain containing protein 3A (ATAD3A) is a nuclear-encoded mitochondrial membrane protein, which is essential for cell growth and metabolism. The mechanism by which ATAD3A acts is still not fully understood. In this study, we explored the regulatory role of ATAD3A on milk biosynthesis and proliferation of bovine mammary epithelial cell. Read More

    Pathological lesions and global DNA methylation in rat prostate under streptozotocin-induced diabetes and melatonin supplementation.
    Cell Biol Int 2017 Dec 26. Epub 2017 Dec 26.
    Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, Univ Estadual Paulista - UNESP, 15054-970, São José do Rio Preto, SP, Brazil.
    Chronic hyperglycemia increases production of reactive oxygen species, which favors carcinogenesis. The association between diabetes and prostate cancer is controversial. Melatonin has antioxidant, anti-inflammatory and antiproliferative properties. Read More

    Anti-proliferative effect of rosiglitazone in the human T-lymphocyte leukaemia cell line Jurkat cells.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    Department of Endocrinology and Metabolism, Peking University Third Hospital, Beijing, China.
    Peroxisome proliferator-activated receptor γ (PPARγ) is expressed in various types of human cancer cells including leukaemia cells, and activation of PPARγ can inhibit cancer cell growth. However, whether PPARγ is expressed in Jurkat cells, a human T-lymphocyte leukaemia cell line, and whether activation of PPARγ affects cell biological behaviours remains to be clarified. In this study, we investigated the effect of a PPARγ activator rosiglitazone, under clinically relevant pharmacological concentrations, on the growth and apoptosis of Jurkat cells in vitro and explored the possible mechanism. Read More

    Effect of ECM2 Expression on Bovine Skeletal Muscle-Derived Satellite Cell Differentiation.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
    Extracellular matrix components have important regulatory functions during cell proliferation and differentiation. In recent study, extracellular matrix were shown to have a strong effect on skeletal muscle differentiation. Here, we aimed to elucidate the effects of extracellular matrix protein 2 (ECM2), an extracellular matrix component, on the differentiation of bovine skeletal muscle-derived satellite cells (MDSCs). Read More

    Activation of KLF4 expression by small activating RNA promotes migration and invasion in colorectal epithelial cells.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    The Center for Reproductive medicine, the First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Er Road, Guangzhou 510080, China.
    RNA activation mediated by small double-stranded RNAs targeting promoter sequence named small activating RNAs (saRNAs), is one of the mechanisms for gene activation. Artificial regulation of gene expression through RNA activation doesn't affect the alteration of the genomic DNA sequences or exogenous plasmid DNA, therefore it is a relative manageable approach for gene perturbation. KLF4 is a member of zinc-finger transcription factors and its functions in colorectal cells are still controversial. Read More

    miR-200a enhances TRAIL-induced apoptosis in gastric cancer cells by targeting A20.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    Department of Medical Oncology, the First Hospital of China Medical University, Shenyang 110001, China.
    Tumor necrosis factor-alpha-related apoptosis-inducing ligand (TRAIL) triggers apoptosis by inducing the death-inducing signaling complex (DISC) formation. Recently, TNFα-induced protein 3 (TNFAIP3, A20) was reported to prevent TRAIL-induced caspase 8 cleavage in the DISC by mediating ubiquitination of RIP1 in glioblastoma. However, whether A20 regulates caspase 8 cleavage in the DISC when TRAIL induces apoptosis in gastric cancer cells is unknown. Read More

    Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309#, Shanghai, 200237, P. R. China.
    Human amniotic membrane-derived mesenchymal stem cells (hAMSCs) draw great interests for regenerative medicine due to convenient availability and low immunogenicity. However, suboptimal culture conditions limit their application. In recent years, small molecules have proven powerful in regulating stem cell fates and can be applied to stimulate their function. Read More

    The delta opioid peptide D-Alanine 2, Leucine 5 Enkephaline (DADLE)-induces neuroprotection through cross-talk between the UPR and pro-survival MAPK-NGF-Bcl2 signalling pathways via modulation of several micro-RNAs in SH-SY5Y cells subjected to ER stress.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT) University, Vellore 632014, Tamil Nadu, India.
    Parkinson's disease (PD) is the second most progressive neurodegenerative disease characterized by the loss of dopaminergic neurons and accumulation of misfolded proteins in endoplasmic reticulum (ER) leading to activation of the unfolded protein response (UPR). In the present study, we aimed to determine the potential survival effect of the delta opioid neuro-peptide D-Alanine 2, Leucine 5 Enkephaline (DADLE) and its mechanism in dopaminergic SH-SY5Y cells which were subjected to ER stress. In this cellular model of PD, enhanced cell survivability was observed on DADLE treatment (but not with μ and κ opioid agonists) along with concomitant down regulation of the UPR stress sensors and protein aggregates. Read More

    Role of mitochondrial dysfunction in the pathophysiology of DNA repair disorders.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
    DNA is constantly being damaged, either by endogenous or exogenous genotoxins. In that regard, DNA repair activities are essential for maintaining genomic stability and to life itself. Mutations in genes encoding DNA repair proteins cause severe human syndromes, but DNA repair defects have also been linked to several other diseases, notably to cancer and normal aging. Read More

    Differentiation of female Oct4-GFP embryonic stem cells into germ lineage cells.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR.
    Due to high infertility ratio nowadays, it is essential to explore efficient ways of enhancing mammalian reproductivity, in particular female reproductivity. Using female Oct4-GFP embryonic stem cells, we mimic the in vivo development procedure to induce ES cells into epiblast cell-like cells (EpiLCs) and then primordial germ cell-like cells (PGCLCs). GFP positive PGCLCs that showed typical PGC markers and epigenetic modification were efficiently obtained. Read More

    Mitoimmunity - When Mitochondria Dictates Macrophage Function.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Laboratory of Immunometabolism. Department of Genetics, Evolution, and Bioagents - Institute of Biology - University of Campinas, São Paulo, Brazil.
    In the past decade, several reports have appointed the importance of mitochondria in the immune response. Our understanding of mitochondria evolved from a simple supplier of energy into a platform necessary for immunorregulation. Proinflammatory responses are associated with enhanced glycolytic activity and breakdown of the TCA cycle. Read More

    Chk1 activation attenuates sensitivity of lapatinib in HER2-positive gastric cancer.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Department of Medical Oncology, the First Hospital of China Medical University, Shenyang 110001, China.
    The ATM and Rad3-related (ATR) /checkpoint kinase 1 (Chk1) pathway plays a pivotal role in DNA damage sensor and modulating homologous recombination. Recently, emerging evidence demonstrated that Chk1 phosphorylation was associated with chemotherapy and radiotherapy resistance. In this study, we explored the effect of ATR/Chk1 pathway on regulating lapatinib sensitivity in human epidermal growth factor receptor-2 (HER2)-positive gastric cancer cell lines. Read More

    Extracellular matrix remodeling and TGF-β1/Smad signaling in diabetic colon mucosa.
    Cell Biol Int 2017 Dec 11. Epub 2017 Dec 11.
    Instituto Superior de Investigaciones Biológicas (INSIBIO) Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Tucumán (CONICET-UNT), Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina.
    Diabetes is associated with metabolic and functional alterations in the gut. Using an experimental model of streptozotocin (STZ)-induced diabetes in rodents, we analyzed the extracellular matrix (ECM) and TGF-β/Smad signaling in the colon mucosa. Male rats were divided into normal control, diabetic and insulin treated diabetic groups during 4 and 9 weeks. Read More

    Mechanisms of AMPK in the maintenance of ATP balance during energy metabolism.
    Cell Biol Int 2017 Dec 4. Epub 2017 Dec 4.
    Department of Gastroenterology, Research Institute of Digestive Diseases, The First Affiliated Hospital of Nanchang University, 17th Yongwaizheng St., Nanchang, Jiangxi, 330006, China.
    AMP-activated protein kinase (AMPK) is a conserved sensor of cellular energy change and is activated by increased AMP/ATP and/or ADP/ATP ratios. AMPK maintains the energy balance by decreasing the ATP-consuming processes such as transcription of synthetic fat genes and rRNA, the translation of ribosomal proteins, synthesis of cholesterol and fatty acid, while the metabolic pathways such as glucose and fatty transport, fatty acid oxidation, autophagy, mitochondrial synthesis and oxidative metabolism are increased to preserve ATP during energy deficiency. Recent advance has demonstrated that AMPK activity has a close association with the initiation and progression in various cancers. Read More

    Silencing of gelatinase expression delays myoblast differentiation in vitro.
    Cell Biol Int 2017 Nov 28. Epub 2017 Nov 28.
    Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, 1 Miecznikowa St., 02-096, Warsaw, Poland.
    Skeletal muscle growth and regeneration relies on the activation of muscle specific stem cells, that is, satellite cells. The activation and differentiation of satellite cells into myoblasts, as well as their migration, proliferation, and fusion of mononuclear myoblasts into a functional multi-nucleated muscle fiber, are associated with extracellular matrix (ECM) protein synthesis and degradation. The extracellular environment is dynamically adapting to the changes accompanying skeletal muscle growth or repair. Read More

    Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits.
    Cell Biol Int 2017 Nov 21. Epub 2017 Nov 21.
    Departamento de Microbiologia, Universidade de São Paulo, São Paulo, Brazil.
    Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. Read More

    PKG II effectively reversed EGF-induced protein expression alterations in human gastric cancer cell lines.
    Cell Biol Int 2017 Nov 18. Epub 2017 Nov 18.
    Medical School, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, P. R. China.
    Epidermal growth factor receptor (EGFR) plays an important role in gastric cancer (GC) progression. Our previous data demonstrated that type II cGMP-dependent protein kinase (PKG II) could block the EGF-EGFR axis as well as down-stream signaling pathways, for example, MAPK, PI3 K, and PLC in GC cells. However, the exact mechanisms of PKG II against cancer remain unclear. Read More

    MCP2 activates NF-κB signaling pathway promoting the migration and invasion of ESCC cells.
    Cell Biol Int 2017 Nov 17. Epub 2017 Nov 17.
    Tumor Hospital Affiliated to Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, P.R. China.
    MCP2, aliased CCL8, has been suggested to be implicated in the metastasis of cancer cells; however, no direct evidence has been established in esophageal squamous cell carcinoma (ESCC). In our present study, to investigate the role MCP2 played in the metastasis of ESCC cells; in vitro cell co-culture system was established. Wound-healing and Transwell assays were used to evaluate the migratory and invasive variation of ESCC cells before and after treatment with recombinant human MCP2. Read More

    Metabolic profiling of normal hepatocyte and hepatocellular carcinoma cells via 1 H nuclear magnetic resonance spectroscopy.
    Cell Biol Int 2017 Nov 16. Epub 2017 Nov 16.
    Department of Radiology, Fujian Provincial Cancer Hospital, Fuzhou, 350014, China.
    Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Read More

    Co-culturing porcine normal urothelial cells, urinary bladder fibroblasts and smooth muscle cells for tissue engineering research.
    Cell Biol Int 2017 Nov 8. Epub 2017 Nov 8.
    Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.
    New strategies for culturing and co-culturing of the main types of urinary bladder cells are essential for successful establishment of biomimetic in vitro models, which could be applied for research into, and management of, diverse urological disorders. Porcine normal urothelial cells are available in nearly unlimited amounts and have many properties equivalent to human urothelial cells. In the present study, we established normal differentiated porcine urothelial cells in co-cultures with porcine urinary bladder normal fibroblasts and/or smooth muscle cells. Read More

    Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary.
    Cell Biol Int 2017 Nov 7. Epub 2017 Nov 7.
    Provincial Key Laboratory of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, #1 Beijing East Road, Wuhu, Anhui, 241000, P.R. China.
    The Drosophila ovary provides an attractive model for studying the extrinsic or intrinsic factors that regulate the fate of germline stem cells (GSCs). Using this model, we identified a new role for Drosophila spaghetti (spag), encoding a homolog of human RNA polymerase II-associated protein 3 (RPAP3), in regulating the fate of ovarian GSCs. Results from spag knockdown and genetic mosaic studies suggest that spag functions as an intrinsic factor for GSCs maintenance. Read More

    In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines.
    Cell Biol Int 2017 Nov 4. Epub 2017 Nov 4.
    Division of Anatomy and Physiopathology, Department of Clinical and Experimental Sciences, University of Brescia, V.le Europa 11, Brescia, 25123, Italy.
    The aim of this study was to investigate the in vitro effect of Silicon, in the soluble form of sodium orthosilicate, combined and not with the concentrated growth factors (CGF), a platelet-rich preparation, on three different human cell lines of fibroblasts (NHDF), endothelial cells (HUVEC), and osteoblasts (HOBs). Each cell type was treated with sodium orthosilicate at the final concentration of 0.5 mM and 1 mM, CGF, and sodium orthosilicate combined with CGF, for 72 h. Read More

    SODD promotes glucose uptake of colorectal cancer cells via AKT pathway.
    Cell Biol Int 2017 Oct 31. Epub 2017 Oct 31.
    Department of Gastric Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Insititute.
    Altered glucose metabolism has been found in a variety of malignant diseases and recognized as one of the hallmarks of cancer. However, the molecular mechanism behind it is far from well understood. In current study, we found silencer of death domains (SODD) regulates glucose uptake of colorectal cancer cells. Read More

    MiR-29b expression is associated with a dexmedetomidine-mediated protective effect against oxygen-glucose deprivation-induced injury to SK-N-SH cells in vitro.
    Cell Biol Int 2017 Oct 31. Epub 2017 Oct 31.
    Key Laboratory of Endemic and Ethnic Diseases, The Key Laboratory of Medical Molecular Biology in Guizhou Medical University, Guiyang, 550002, China.
    Ischemic cerebral stroke is a leading cause of death and long-term disability world-wide. Neuronal injury following cerebral ischemia initiates a complex series of signaling cascades that lead to neuronal cell death. MicroRNA 29b (miR-29b) has reported involvement in the pathogenic process of ischemic brain injury. Read More

    Cancer and normal adipose-derived mesenchymal stem cells (ASCs): Do they have differential effects on tumor and immune cells?
    Cell Biol Int 2017 Oct 27. Epub 2017 Oct 27.
    Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
    Adipose-derived mesenchymal stem cells (ASCs) are known to have immunomodulatory properties through soluble factors or by direct cell-to-cell contact. This study aimed to assess the expression of HLA-G and IDO activity in breast cancer and normal ASCs and to see whether ASC is capable of modulating both tumor cells and immune system cells in vitro. ASCs were enzymatically isolated from 15 breast cancer patients and 10 normal individuals. Read More

    Desipramine-induced lysosomal vacuolization is independent of autophagy.
    Cell Biol Int 2017 Oct 25. Epub 2017 Oct 25.
    Department of Internal Medicine, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, Japan.
    Desipramine, a commonly used antidepressant drug, induced cytosolic vacuolization in L929 cells. The level of LC3-II was elevated and that of p62 was reduced in desipramine-treated L929 cells, indicating the induction of autophagy by desipramine. Surprisingly, massive vacuolization was observed in desipramine-treated L929 cells in the presence of LY294002, an inhibitor of autophagy. Read More

    Isolation and characterization of human mesenchymal stem cells derived from synovial fluid by magnetic-activated cell sorting (MACS).
    Cell Biol Int 2017 Oct 25. Epub 2017 Oct 25.
    Guangzhou Medical University, Guangzhou 510095, Guangdong Province, China.
    Mesenchymal stem cells (MSCs) are the primary source of cells used for cell-based therapy in tissue engineering. MSCs are found in synovial fluid, a source that could be conveniently used for cartilage tissue engineering. However, the purification and characterization of SF-MSCs has been poorly documented in the literature. Read More

    Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα-knockdown cells.
    Cell Biol Int 2017 Oct 25. Epub 2017 Oct 25.
    Department of Endodontics, School of Stomatology, China Medical University, Nanjing North Street 117, Shenyang, 110002, China.
    Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-related transcription factor 2 (Runx2). We previously reported that knockdown of PP2A Cα increases the expression of Osterix, leading to the accelerated osteoblast differentiation through the upregulation of bone-related genes. Read More

    The micronome of mesenchymal stromal cells is partially responsive to inflammation.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Université Libre de Bruxelles (ULB), Campus Erasme, Brussels, Belgium.
    Mesenchymal stromal cells (MSCs) display a special immunological profile that allows their potential use as immunotherapeutic cells. Nowadays, foreskin (FSK) represents a valuable reservoir of MSCs with International Society for Cellular Therapy (ISCT) compliant criteria and relevant functional properties. However, their mode of action is poorly understood and needs to be more elucidated to optimize their therapeutic use. Read More

    Histone deacetylase inhibitor stimulates E2 and P4 secretion in sika deer ovarian granulosa cells at a moderate dose.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
    The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. Read More

    Contribution to mitochondrial research in Brazil: 10th anniversary of the mitomeeting.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Biology Institute, State University of Campinas, 13083-970, Campinas, SP, Brazil.
    This commentary introduces the subject, the context and the history of the Brazilian annually held meeting on Mitochondrial Research by the occasion of its 10th anniversary. Mitomeetings gather people interested in all aspects of mitochondrial biology in diverse species, including protists, animals, plants, and fungi. Read More

    miR-23b-3p and miR-125b-5p downregulate apo(a) expression by targeting Ets1 in HepG2 cells.
    Cell Biol Int 2017 Oct 24. Epub 2017 Oct 24.
    Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan, 421001, PR China.
    High concentrations of plasma lipoprotein(a) [Lp(a)] have been inferred to be an independent risk factor for cardiovascular and cerebrovascular diseases, such as coronary artery diseases, restenosis, and stroke. Apolipoprotein(a) [apo(a)] is one of the most important components of Lp(a) and contributes greatly to the increased concentration of plasma Lp(a). As a critical positive transacting factor of apo(a) gene, Ets1 has been proven as a target gene of several miRNAs, such as miR-193b, miR-125b-5p, miR-200b, miR-1, and miR-499. Read More

    OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, Jiangsu, 213164, P. R. China.
    4β-(1,3,4-oxadiazole-2-amino-5-methyl)-4-deoxypodophyllotoxin (OAMDP), a novel podophyllotoxin derivative, has demonstrated potent anti-tumor activity with significant cytotoxic effect. Here, we report the anti-proliferative effect of OAMDP, for which OAMDP could suppress the proliferation of HepG2 cells (human hepatoma cell line) in a dose- and time-dependent manner. After treating with OAMDP, cell apoptosis was confirmed by Annexin V-FITC/PI double staining assay. Read More

    HBxAg suppresses cell apoptosis and promotes the secretion of placental hormones in human placental trophoblasts via activation of the EGFR/Akt pathway.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    Department of Gynecology and Obstetrics, the First Affiliated Hospital of Xi'an Jiaotong University, No. 277, Yanta West Road, Xi'an City, 710061, Shaanxi Province, China.
    The aim of this study is to investigate the role of Hepatitis B virus x (HBx) in the growth and secretion of human placental trophoblasts. Firstly, placenta tissues were collected from pregnant HBV carriers with various viral loads. The results of immunohistochemical technique showed that the HBx protein and pEGFR protein levels were both markedly increased with the viral load elevation. Read More

    Carnosine and N-acetyl cysteine protect against sodium nitrite-induced oxidative stress in rat blood.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    Faculty of Life Sciences, Department of Biochemistry, Aligarh Muslim University, Aligarh, 202002, UP, India.
    Sodium nitrite (NaNO2 ) is widely used in the food industry as a preservative and colorant in meat and fish products. Industrialization and improper agricultural practices have greatly increased human exposure to high nitrite levels, mainly through contaminated drinking water, causing various health disorders. We have investigated the protective effect of carnosine (CAR) and N-acetyl cysteine (NAC) on NaNO2 -induced toxicity in rat blood. Read More

    Src promotes EGF-induced epithelial-to-mesenchymal transition and migration in gastric cancer cells by upregulating ZEB1 and ZEB2 through AKT.
    Cell Biol Int 2017 Oct 20. Epub 2017 Oct 20.
    Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, Liaoning, 110001, P.R. China.
    Epithelial-to-mesenchymal transition (EMT) plays important roles in the migration, invasion, and metastasis of cancer cells. However, the role of Src in epidermal growth factor (EGF)-induced EMT and migration in gastric cancer cells remains to be clarified. In the current study, the effect of Src on EGF-stimulated EMT and migration was explored in gastric cancer cells. Read More

    A novel HIF1AN substrate KANK3 plays a tumor-suppressive role in hepatocellular carcinoma.
    Cell Biol Int 2017 Oct 19. Epub 2017 Oct 19.
    Department of Biomedical Sciences, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.
    The KN motif and ankyrin repeat domain-containing protein (KANK) family is involved in actin cytoskeleton organization and cell motility. Compared with other KANK members, the biological function of KANK3 is not clear. Here, we identified KANK3 as a new substrate for the oxygen sensor hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), which hydroxylates HIF-1/2α and other ankyrin repeat domain-containing proteins at asparagine residues. Read More

    High magnesium prevents matrix vesicle-mediated mineralization in human bone marrow-derived mesenchymal stem cells via mitochondrial pathway and autophagy.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, 22903.
    Magnesium, as a physiological calcium antagonist, plays a vital role in the bone metabolism and the balance between magnesium and calcium is crucial in bone physiology. We recently demonstrated that matrix mineralization in human bone marrow-derived mesenchymal stem cells (hBMSCs) can be suppressed by high Mg2+ . However, a complete understanding of the mechanisms involved still remains to be elucidated. Read More

    Down-regulation of LncRNA CCAT1 enhances radiosensitivity via regulating miR-148b in breast cancer.
    Cell Biol Int 2017 Oct 11. Epub 2017 Oct 11.
    Department of Oncology, The People's Hospital of Jianyang City, No.180 Hospital Road, Sichuan, 641400, China.
    LncRNA colon-cancer-associated transcript-1 (CCAT1) was proved to be a potential prognostic biomarker for breast cancer progression. However, the role of CCAT1 in regulating radiosensitivity of breast cancer and its underlying mechanism have not been investigated. The present study showed that CCAT1 was up-regulated and miR-148b was down-regulated in radioresistant breast cancer tissues compared with radiosensitive breast cancer tissues. Read More

    CacyBP/SIP inhibits the migration and invasion behaviors of glioblastoma cells through activating Siah1 mediated ubiquitination and degradation of cytoplasmic p27.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    School of Medicine, Shandong University, Jinan, 250012, China.
    Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP) has been reported to be up-regulated and plays an important role in promoting cell proliferation in human glioma. However, the effect of CacyBP/SIP on glioma cell motility is still unclear. Here, to our surprise, CacyBP/SIP was found to inhibit the migration and invasion of glioma cells U251 and U87. Read More

    Structural and functional features of lysine acetylation of plant and animal tubulins.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    Laboratory of Structural Biology, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, Ukraine.
    The study of the genome and the proteome of different species and representatives of distinct kingdoms, especially detection of proteome via wide-scaled analyses has various challenges and pitfalls. Attempts to combine all available information together and isolate some common features for determination of the pathway and their mechanism of action generally have a highly complicated nature. However, microtubule (MT) monomers are highly conserved protein structures, and microtubules are structurally conserved from Homo sapiens to Arabidopsis thaliana. Read More

    Intron length polymorphism of β-tubulin genes of Aegilops biuncialis Vis.
    Cell Biol Int 2017 Oct 10. Epub 2017 Oct 10.
    Institute of Food Biotechnology and Genomics, Osipovskogo St., 2a, Kyiv-123, 04123, Ukraine.
    Intron-specific DNA polymorphism is present among plant β-tubulin gene family members and is considered to be one of the molecular markers based on the difference of tubulin introns length assayed both separately (TBP: 1st intron) or in combination (h-TBP: 1st and 2nd introns). These two approaches are possibly useful for wheat breeding programs, since TBP and h-TBP help to differentiate between the accessions of Aegilops biuncialis Vis., a wild relative of wheat. Read More

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