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    Down-regulation of the non-coding RNA H19 and its derived miR-675 is concomitant with up-regulation of insulin-like growth factor receptor type 1 during neural-like differentiation of human bone marrow mesenchymal stem cells.
    Cell Biol Int 2018 Mar 7. Epub 2018 Mar 7.
    Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
    The differentiation of human bone marrow mesenchymal stem cells (BMSCs) into specific lineages offers new opportunities to use the therapeutic efficiency of these pluripotent cells in regenerative medicine. Multiple lines of evidence have revealed that non-coding RNAs play major roles in the differentiation of BMSCs into neural cells. Here, we applied a cocktail of neural inducing factors (NIFs) to differentiate BMSCs into neural-like cells. Read More

    Ibuprofen reduces cell proliferation through inhibiting Wnt/β catenin signaling pathway in Gastric Cancer Stem Cells.
    Cell Biol Int 2018 Mar 7. Epub 2018 Mar 7.
    Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
    Nowadays, most studies focused on cancer stem cells (CSCs) through their abilities to cause tumorigenicity, drug resistance, and cancer recurrence. On the other side, nonsteroidal anti-inflammatory drugs (NSAIDs) have been taken into consideration because of cheapness and availability. For the reasons mentioned above, we have studied the effect of ibuprofen as an NSAID on CSCs derived from AGS and MKN-45 gastric cancer cell lines to perform effective cancer therapy. Read More

    Pseudomonas aeruginosa infection alters the macrophage phenotype switching process during wound healing in diabetic mice.
    Cell Biol Int 2018 Mar 7. Epub 2018 Mar 7.
    Shanghai Tenth People's Hospital and Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, China.
    Macrophages play critical roles in wound healing process. They switch from "classically activated" (M1) phenotype in the early inflammatory phase to "alternatively activated" (M2) phenotype in the later healing phase. However, the dynamic process of macrophage phenotype switching in diabetic wounds burdened with bacteria is unclear. Read More

    Biological effects of trans fatty acids and their possible roles in the lipid rafts in apoptosis regulation.
    Cell Biol Int 2018 Mar 3. Epub 2018 Mar 3.
    Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, 202, Gongye North Road, Jinan, 250100, PR China.
    A large number of recent studies are focused on evaluating the mechanism of action of trans fatty acids (TFAs) on the progression of apoptosis. A strong positive association has been reported between TFA and coronary heart disease (CHD), obesity and nonalcoholic steatohepatitis and so on. The present study reviewed the biological effects of trans fatty acids (TFA) and their possible roles in lipid rafts in regulating apoptosis. Read More

    Yeast Membrane Lipid Imbalance Leads to Trafficking Defects toward the Golgi.
    Cell Biol Int 2018 Mar 3. Epub 2018 Mar 3.
    Missouri State University, 901 S National Ave., Springfield, Missouri, United States.
    Protein recycling is an essential cellular process involving endocytosis, intracellular trafficking, and exocytosis. In mammalian systems membrane lipids, including cholesterol, sphingolipids, and phospholipids, play a pivotal role in protein recycling. To address this role in budding yeast, Saccharomyces cerevisiae, we utilized GFP-Snc1, a v-SNARE protein serving as a fluorescent marker for faithfully reporting the recycling pathway. Read More

    Icariin attenuates titanium particle-induced inhibition of osteogenic differentiation and matrix mineralization via miR-21-5p.
    Cell Biol Int 2018 Mar 3. Epub 2018 Mar 3.
    Department of Orthopaedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, People's Republic of China.
    Inhibition of bone regeneration by wear debris is the main cause of peri-prosthetic osteolysis. Here, we investigated the effect of icariin on cell proliferation, apoptosis, osteogenic differentiation and matrix mineralization of osteoblasts in an in vitro model of titanium (Ti) particle-induced osteolysis. In the present study, MC3T3-E1 cells were pretreated with 10M icariin for 4 h and then incubated with Ti particles (0. Read More

    Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
    Cell Biol Int 2018 Feb 21. Epub 2018 Feb 21.
    Department of Obstetrics and Gynecology, The First People's Hospital of Yunnan Province, No.157 JinBi Road, Xishan District, Kunming City, Yunnan Province, 650000, China.
    Embryonic development depends on the normal invasion of trophoblast cells. Forkhead box C2 (FOXC2) is a member of Forkhead box family, which is involved in the tumor cells invasion. The aim of this study was to explore the roles of FOXC2 on the invasion of human trophoblast cells, and further study its molecular mechanism. Read More

    Angelica sinensis polysaccharide inhibits proliferation, migration and invasion by down-regulating microRNA-675 in human neuroblastoma cell line SH-SY5Y.
    Cell Biol Int 2018 Feb 21. Epub 2018 Feb 21.
    Department of Pediatric Hematology, The Affiliated Hospital of Qingdao University, Qingdao 266000, China.
    Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the underlying mechanisms. Read More

    Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques.
    Cell Biol Int 2018 Feb 19. Epub 2018 Feb 19.
    Physiology & Developmental Biology, Brigham Young University, Provo, Utah, 84602, USA.
    CRISPR-Cas technology has revolutionized genome engineering. While Cas9 was not the first programmable endonuclease identified, its simplicity of use has driven widespread adoption in a short period of time. While CRISPR-Cas genome editing holds enormous potential for clinical applications, its use in laboratory settings for genotype-phenotype studies and genome-wide screens has led to breakthroughs in the understanding of many molecular pathways. Read More

    Effect of PPARG on AGEs-induced AKT/MTOR signaling-associated human chondrocytes autophagy.
    Cell Biol Int 2018 Feb 17. Epub 2018 Feb 17.
    Department of Orthopedics, The Second Affiliated Hospital of Hunan Normal University, The 163rd Central Hospital of the People's Liberation Army, Changsha, Hunan, 410003, People's Republic of China.
    Accumulation of advanced glycation end products (AGEs) in articular cartilage is thought to represent a major risk factor for osteoarthritis development. In this study we aimed to probe the role of AGEs in human chondrocytes and to determine the impact of the peroxisome proliferator-activated receptor-γ (PPARG) on AGEs-induced cell autophagy. Cell viability was measured after human chondrocytes were treated with different concentrations of AGEs with or without the PPARG inhibitor, T0070907, or agonist, pioglitazone. Read More

    Mangifera indica L. extract (Vimang®) reduces plasma and liver cholesterol and leucocyte oxidative stress in hypercholesterolemic LDL receptor deficient mice.
    Cell Biol Int 2018 Feb 10. Epub 2018 Feb 10.
    Department of Structural and Functional Biology, Biology Institute, State University of Campinas, Campinas, São Paulo, Brazil.
    Cardiovascular diseases are major causes of death worldwide. Beyond the classical cholesterol risk factor, other conditions such as oxidative stress are well documented to promote atherosclerosis. The Mangifera indica L. Read More

    Facilitation of Ca-induced opening of the mitochondrial permeability transition pore either by nicotinamide nucleotide transhydrogenase deficiency or statins treatment.
    Cell Biol Int 2018 Feb 9. Epub 2018 Feb 9.
    Faculdade de Ciências Médicas, Departamento de Patologia Clínica, UNICAMP, Rua Cinco de Junho, 350 CEP 13083-877, Campinas, SP, Brasil.
    Mitochondrial redox imbalance and high Cauptake induce the opening of the permeability transition pore (PTP) that leads to disruption of energy-linked mitochondrial functions and triggers cell death in many disease states. In this review, we discuss the major results from our studies investigating the consequences of NAD(P)-transhydrogenase (NNT) deficiency, and of statins treatment for mitochondrial functions and susceptibility to Ca-induced PTP. We highlight the aggravation of high fat diet-induced fatty liver disease in the context of NNT deficiency and the role of antioxidants in the prevention of statins toxicity to mitochondria. Read More

    The role of mitochondria in the female germline: Implications to fertility and inheritance of mitochondrial diseases.
    Cell Biol Int 2018 Feb 8. Epub 2018 Feb 8.
    Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, 13565-905, Brazil.
    Mitochondria play a fundamental role during development of the female germline. They are fragmented, round, and small. Despite these characteristics suggesting that they are inactive, there is accumulating evidence that mitochondrial dysfunctions are a major cause of infertility and generation of aneuploidies in humans. Read More

    Emerging role of the Jun N-terminal kinase interactome in human health.
    Cell Biol Int 2018 Feb 8. Epub 2018 Feb 8.
    Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
    The c-Jun N-terminal kinases (JNKs) are located downstream of Ras-mitogen activated protein kinase signaling cascades. More than 20 years of study has shown that JNKs control cell fate and many cellular functions. JNKs and their interacting proteins form a complicated network with diverse biological functions and physiological effects. Read More

    Circulating molecules that control brown/beige adipocyte differentiation and thermogenic capacity.
    Cell Biol Int 2018 Jan 31. Epub 2018 Jan 31.
    Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
    Obesity may be counteracted by increased energy expenditure. Circulating molecules act in the adipose tissue to influence brown and beige adipocyte function, differentiation, and thermogenic capacity, which in turn affects substrate utilization and impacts energy balance at the organismal level. These molecules have been envisioned as biomarkers and potential candidates for pharmacological interventions to treat obesity. Read More

    Modulation of mitochondrial metabolism as a biochemical trait in blood feeding organisms: the redox vampire hypothesis redux.
    Cell Biol Int 2018 Jan 31. Epub 2018 Jan 31.
    Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brazil.
    Hematophagous organisms undergo remarkable metabolic changes during the blood digestion process, increasing fermentative glucose metabolism, and reducing respiratory rates, both consequence of functional mitochondrial remodeling. Here, we review the pathways involved in energy metabolism and mitochondrial functionality in a comparative framework across different hematophagous species, and consider how these processes regulate redox homeostasis during blood digestion. The trend across distinct species indicate that a switch in energy metabolism might represent an important defensive mechanism to avoid the potential harmful interaction of oxidants generated from aerobic energy metabolism with products derived from blood digestion. Read More

    Xenotopic expression of alternative electron transport enzymes in animal mitochondria and their impact in health and disease.
    Cell Biol Int 2018 Jan 31. Epub 2018 Jan 31.
    Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista "Júlio de Mesquita Filho", Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
    The mitochondrial respiratory chain in vertebrates and arthropods is different from that of most other eukaryotes because they lack alternative enzymes that provide electron transfer pathways additional to the oxidative phosphorylation (OXPHOS) system. However, the use of diverse experimental models, such as human cells in culture, Drosophila melanogaster and the mouse, has demonstrated that the transgenic expression of these alternative enzymes can impact positively many phenotypes associated with human mitochondrial and other cellular dysfunction, including those typically presented in complex IV deficiencies, Parkinson's, and Alzheimer's. In addition, these enzymes have recently provided extremely valuable data on how, when, and where reactive oxygen species, considered by many as "by-products" of OXPHOS, can contribute to animal longevity. Read More

    NTPDase activities: possible roles on Leishmania spp infectivity and virulence.
    Cell Biol Int 2018 Jan 31. Epub 2018 Jan 31.
    Laboratório de Bioquímica Celular, Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brazil.
    Nucleoside triphosphate diphosphohydrolases (NTPDases) are enzymes that belong to the GDA1/CD39 protein superfamily. These enzymes catalyze the hydrolysis of ATP and ADP to the monophosphate form (AMP). Biochemical characterization of the nucleotidases/NTPDases from various types of cells, including those from plants, animals, and pathogenic organisms, has revealed the existence of several isoforms with different specificities with respect to divalent cations (magnesium, calcium, manganese, and zinc) and substrates. Read More

    The poly-cistronic expression of four transcriptional factors (CRX, RAX, NEURO-D, OTX2) in fibroblasts via retro- or lentivirus causes partial reprogramming into photoreceptor cells.
    Cell Biol Int 2018 Jan 29. Epub 2018 Jan 29.
    Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Iwate Prefecture, 020-8551, Morioka, Japan.
    The introduction of four key transcriptional factors (CRX, RAX, NEURO-D, OTX2) allows the direct differentiation of fibroblasts to retinal photoreceptor cells. This reprogramming was achieved with a combination of mono-cistronic viruses. Although the combination of mono-cistronic viruses was useful, a relatively high titer of recombinant viruses was necessary because co-infections are required. Read More

    Cabazitaxel inhibits proliferation and potentiates the radiation response of U87MG glioblastoma cells.
    Cell Biol Int 2018 Jan 25. Epub 2018 Jan 25.
    Cellular and Molecular Research Center and Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
    Cabazitaxel is a second-generation semisynthetic taxane. The recognized anti-neoplastic effect of Cabazitaxel is cell cycle perturbation by inducing arrest at G2/M. Since glioblastoma tumors have a relatively high expression of P-gp, it is encouraging to find a treatment that is effective against these tumors. Read More

    Sphingomyelin in microdomains of the plasma membrane regulates amino acid-stimulated mTOR signal activation.
    Cell Biol Int 2018 Jan 25. Epub 2018 Jan 25.
    Faculty of Advanced Life Science, Department of Biomembrane and Biofunctional Chemistry, Hokkaido University, Sapporo, 001-0021, Japan.
    Sphingomyelin (SM) is required for cells to proliferate, but the reason is not fully understood. In order to asses this question, we employed a cell line, ZS, which lacks both SMS1 and SMS2, isolated from mouse embryonic fibroblasts in SMS1 and 2 double knockout mouse, and SMS1 or SMS2 re-expressing cells, ZS/SMS1 or ZS/SMS2, respectively. We investigated regulation of SM in activating the mammalian target of rapamycin (mTOR) signal induced by essential amino acids (EAA), using these cells. Read More

    Immune responses of human dental pulp stem cells in lipopolysaccharide induced microenvironment.
    Cell Biol Int 2018 Jan 24. Epub 2018 Jan 24.
    Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
    This study aimed to investigate the effect of inflammatory stimuli on dental pulp stem cells (DPSCs) by assessing their proliferation and expression of genes as well as proteins in lipopolysaccharide (LPS) induced microenvironment (iDPSCs). DPSCs were first characterized for their mesenchymal properties prior to challenging them with a series of LPS concentrations from 12 to 72 hours. Following to this, their proliferation and inflammatory based genes as well protein expression were assessed. Read More

    Resident murine macrophage migration and phagocytosis are modulated by growth hormone.
    Cell Biol Int 2018 Jan 24. Epub 2018 Jan 24.
    Laboratory of Cell Biology, Institute of Biology and Health Science, Federal University of Alagoas, Maceió, CEP 57072-970, Alagoas, Brazil.
    Growth hormone (GH) plays a physiological role in the immune system. In macrophages, GH enhances the production of hydrogen peroxide, superoxide anions, nitric oxide, cytokines, and chemokines, including interferon-γ and macrophage inflammatory protein-1α. However, some of the effects of GH stimulation on the biological functions of macrophages remain to be elucidated. Read More

    WNT5A promotes migration and invasion of human osteosarcoma cells via SRC/ERK/MMP-14 pathway.
    Cell Biol Int 2018 Jan 18. Epub 2018 Jan 18.
    Department of Orthopaedics, Second Hospital of Lanzhou University, Lanzhou, 730000, China.
    WNT5A, a representative ligand of activating several non-canonical WNT signal pathways, plays significant roles in oncogenesis and tumor inhibition. It has been shown that the non-receptor tyrosine kinase SRC is required for WNT5A-induced invasion of osteosarcoma cells. However, the precise molecular mechanism underlying WNT5A/SRC-mediated osteosarcoma cells invasion remains poorly defined. Read More

    Glycine improves the remodeling process of tenocytes in vitro.
    Cell Biol Int 2018 Jan 18. Epub 2018 Jan 18.
    Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-863 CP 6109, Campinas, São Paulo, Brazil.
    Tendinitis changes the biochemical and morphological properties of the tendon, promoting an increase of activity of metalloproteinases and disorganization of collagen bundles. Tenocytes, the primary cells in tendon, are scattered throughout the collagenic fibers, and are responsible of tendon remodeling and tissue repair in pathological condition. In vivo, glycine, component of the typical Gly-X-Y collagen tripeptide, showed beneficial effects in biochemical and biomechanical properties of Achilles tendon with tendinitis. Read More

    NPTX1 inhibits colon cancer cell proliferation through down-regulating cyclin A2 and CDK2 expression.
    Cell Biol Int 2018 Jan 18. Epub 2018 Jan 18.
    Department of Gastroenterology, Huizhou Municipal Central Hospital, Huizhou, People's Republic of China.
    Colon cancer is the third most common malignancy and one of the leading causes of cancer-associated mortality worldwide. Neuronal pentraxin 1 (NPTX1) is associated with tumor progression in some types of tumors. However, its expression and role in colon cancer has not been yet reported. Read More

    Selenocysteine inhibits human osteosarcoma cells growth through triggering mitochondrial dysfunction and ROS-mediated p53 phosphorylation.
    Cell Biol Int 2018 Jan 11. Epub 2018 Jan 11.
    Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jingwu Road 324, Jinan, 250021, Shandong, China.
    Osteosarcoma represents the most common primary malignant bone tumor in children and adolescents, which shows severe resistance toward standard chemotherapy because of high invasive capacity and growing incidence. Selenocysteine (SeC) is a naturally available Se-containing amino acid that displays splendid anticancer activities against several human tumors. However, little information about SeC-induced growth inhibition against human osteosarcoma is available. Read More

    A small population of resident limb bud mesenchymal cells express few MSC-associated markers, but the expression of these markers is increased immediately after cell culture.
    Cell Biol Int 2018 Jan 4. Epub 2018 Jan 4.
    Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México Ciudad Universitaria. Apartado Postal 70228, México.
    Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors, or multipotent mesenchymal stromal cells (MSC). Derived-MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Read More

    Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures.
    Cell Biol Int 2017 Dec 30. Epub 2017 Dec 30.
    Department of Cell Biology and Biotechnology, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Osipovskogo St., 2a, Kyiv, 04123, Ukraine.
    Cytoskeleton is gaining the increasing recognition as one of nitric oxide (NO)-downstream targets because of its involvement in plenty of NO-controlled processes in plants throughout the entire life cycle starting from seed germination to pollination as well as (a)biotic stress tolerance. It has been revealed that low temperature (+0.5°C) has an inhibitory effect on A. Read More

    IL-1α inhibits proliferation and adipogenic differentiation of human adipose-derived mesenchymal stem cells through NF-κB- and ERK1/2- mediated proinflammatory cytokines.
    Cell Biol Int 2017 Dec 30. Epub 2017 Dec 30.
    Institute of Aging Research, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan 523808, Guangdong Province, China.
    Dysfunctional adipogenesis such as subcutaneous lipoatrophy is closely related to insulin resistance and metabolic disorders. Although the expression or release of the cytokine interleukin-1α(L-1α) is known to increase in adipose tissue in response to cell death, cell senescence, aging, or solar radiation, the regulatory role of IL-1α in adipogenesis has not been sufficiently investigated. To investigate the problem, we explored the effect of IL-1α on the proliferation and adipogenic differentiation of human adipose-derived mesenchymal stem cells (ADSCs) using cell counting, alamarBlue assay, oil O staining, Western blot, among others. Read More

    Knockdown of CPEB4 expression suppresses cell migration and invasion via Akt pathway in non-small cell lung cancer.
    Cell Biol Int 2017 Dec 29. Epub 2017 Dec 29.
    Department of Medical Oncology, First People's Hospital of Yunnan Province, No. 157 of Jinbi Road, Xishan district of Kunming City, Yunnan Province, P. R. China.
    Cytoplasmic polyadenylation element binding protein 4 (CPEB4) could be an important regulator in variety of cancers. However, the biological function and the underlying molecular mechanism of CPEB4 in non-small cell lung cancer (NSCLC) remains unknown. In this study, we investigated the metastasis role of CPEB4 in NSCLC cells, we knocked down CPEB4 using siRNA. Read More

    The mitochondrial calcium uniporter complex in trypanosomes.
    Cell Biol Int 2017 Dec 29. Epub 2017 Dec 29.
    Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
    The presence of a conserved mechanism for mitochondrial calcium uptake in trypanosomatids was crucial for the molecular identification of the mitochondrial calcium uniporter (MCU), a long-sought channel present in most eukaryotic organisms. Since then, research efforts to elucidate the role of MCU and its regulatory elements in different biological models have multiplied. MCU is the pore-forming subunit of a multimeric complex (the MCU complex or MCUC) and its predicted structure in trypanosomes is simpler than in mammalian cells, lacking two of its subunits and probably possessing other unidentified components. Read More

    Mitochondrial ATAD3A regulates milk biosynthesis and proliferation of mammary epithelial cells from dairy cow via the mTOR pathway.
    Cell Biol Int 2017 Dec 29. Epub 2017 Dec 29.
    The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin, 150030, China.
    ATPase family AAA-domain containing protein 3A (ATAD3A) is a nuclear-encoded mitochondrial membrane protein, which is essential for cell growth and metabolism. The mechanism by which ATAD3A acts is still not fully understood. In this study, we explored the regulatory role of ATAD3A on milk biosynthesis and proliferation of bovine mammary epithelial cell. Read More

    Pathological lesions and global DNA methylation in rat prostate under streptozotocin-induced diabetes and melatonin supplementation.
    Cell Biol Int 2018 Apr 8;42(4):470-487. Epub 2018 Feb 8.
    Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, Univ Estadual Paulista-UNESP, 15054-970, São José do Rio Preto, SP, Brazil.
    Chronic hyperglycemia increases production of reactive oxygen species, which favors carcinogenesis. The association between diabetes and prostate cancer is controversial. Melatonin has antioxidant, anti-inflammatory, and antiproliferative properties. Read More

    Anti-proliferative effect of rosiglitazone in the human T-lymphocyte leukaemia cell line Jurkat cells.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    Department of Endocrinology and Metabolism, Peking University Third Hospital, Beijing, China.
    Peroxisome proliferator-activated receptor γ (PPARγ) is expressed in various types of human cancer cells including leukaemia cells, and activation of PPARγ can inhibit cancer cell growth. However, whether PPARγ is expressed in Jurkat cells, a human T-lymphocyte leukaemia cell line, and whether activation of PPARγ affects cell biological behaviors remains to be clarified. In this study, we investigated the effect of a PPARγ activator rosiglitazone, under clinically relevant pharmacological concentrations, on the growth and apoptosis of Jurkat cells in vitro and explored the possible mechanism. Read More

    Effect of ECM2 expression on bovine skeletal muscle-derived satellite cell differentiation.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    The Laboratory of Cell and Development, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang Strict, Harbin, Heilongjiang, 150030, China.
    Extracellular matrix components have important regulatory functions during cell proliferation and differentiation. In recent study, extracellular matrix were shown to have a strong effect on skeletal muscle differentiation. Here, we aimed to elucidate the effects of extracellular matrix protein 2 (ECM2), an extracellular matrix component, on the differentiation of bovine skeletal muscle-derived satellite cells (MDSCs). Read More

    Activation of KLF4 expression by small activating RNA promotes migration and invasion in colorectal epithelial cells.
    Cell Biol Int 2018 Apr 22;42(4):495-503. Epub 2018 Jan 22.
    The Center for Reproductive medicine, the First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Er Road, Guangzhou, 510080, China.
    RNA activation mediated by small double-stranded RNAs targeting promoter sequence named small activating RNAs (saRNAs) is one of the mechanisms for gene activation. Artificial regulation of gene expression through RNA activation does not affect the alteration of the genomic DNA sequences or exogenous plasmid DNA, therefore it is a relative manageable approach for gene perturbation. KLF4 is a member of zinc-finger transcription factors and its functions in colorectal cells are still controversial. Read More

    miR-200a enhances TRAIL-induced apoptosis in gastric cancer cells by targeting A20.
    Cell Biol Int 2017 Dec 23. Epub 2017 Dec 23.
    Department of Medical Oncology, the First Hospital of China Medical University, Shenyang, 110001, China.
    Tumor necrosis factor-alpha-related apoptosis-inducing ligand (TRAIL) triggers apoptosis by inducing the death-inducing signaling complex (DISC) formation. Recently, TNFα-induced protein 3 (TNFAIP3, A20) was reported to prevent TRAIL-induced caspase 8 cleavage in the DISC by mediating ubiquitination of RIP1 in glioblastoma. However, whether A20 regulates caspase 8 cleavage in the DISC when TRAIL induces apoptosis in gastric cancer cells is unknown. Read More

    Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate.
    Cell Biol Int 2018 Apr 14;42(4):457-469. Epub 2018 Jan 14.
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309#, Shanghai, 200237, P. R. China.
    Human amniotic membrane-derived mesenchymal stem cells (hAMSCs) draw great interests for regenerative medicine due to convenient availability and low immunogenicity. However, suboptimal culture conditions limit their application. In recent years, small molecules have proven powerful in regulating stem cell fates and can be applied to stimulate their function. Read More

    The delta opioid peptide D-Alanine 2, Leucine 5 Enkephaline (DADLE)-induces neuroprotection through cross-talk between the UPR and pro-survival MAPK-NGF-Bcl2 signaling pathways via modulation of several micro-RNAs in SH-SY5Y cells subjected to ER stress.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, 632014, India.
    Parkinson's disease (PD) is the second most progressive neurodegenerative disease characterized by the loss of dopaminergic neurons and accumulation of misfolded proteins in endoplasmic reticulum (ER) leading to activation of the unfolded protein response (UPR). In the present study, we aimed to determine the potential survival effect of the delta opioid neuro-peptide D-Alanine 2, Leucine 5 Enkephaline (DADLE), and its mechanism in dopaminergic SH-SY5Y cells which were subjected to ER stress. In this cellular model of PD, enhanced cell survivability was observed on DADLE treatment (but not with μ and κ opioid agonists) along with concomitant down regulation of the UPR stress sensors and protein aggregates. Read More

    Role of mitochondrial dysfunction in the pathophysiology of DNA repair disorders.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
    DNA is constantly being damaged, either by endogenous or exogenous genotoxins. In that regard, DNA repair activities are essential for maintaining genomic stability and to life itself. Mutations in genes encoding DNA repair proteins cause severe human syndromes, but DNA repair defects have also been linked to several other diseases, notably to cancer and normal aging. Read More

    Differentiation of female Oct4-GFP embryonic stem cells into germ lineage cells.
    Cell Biol Int 2018 Apr 17;42(4):488-494. Epub 2018 Jan 17.
    Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China.
    Due to high infertility ratio nowadays, it is essential to explore efficient ways of enhancing mammalian reproductivity, in particular female reproductivity. Using female Oct4-GFP embryonic stem cells, we mimic the in vivo development procedure to induce ES cells into epiblast cell-like cells (EpiLCs) and then primordial germ cell-like cells (PGCLCs). GFP positive PGCLCs that showed typical PGC markers and epigenetic modification were efficiently obtained. Read More

    Mitoimmunity-when mitochondria dictates macrophage function.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, São Paulo, Brazil.
    In the past decade, several reports have appointed the importance of mitochondria in the immune response. Our understanding of mitochondria evolved from a simple supplier of energy into a platform necessary for immunorregulation. Proinflammatory responses are associated with enhanced glycolytic activity and breakdown of the TCA cycle. Read More

    Chk1 activation attenuates sensitivity of lapatinib in HER2-positive gastric cancer.
    Cell Biol Int 2017 Dec 22. Epub 2017 Dec 22.
    Department of Medical Oncology, the First Hospital of China Medical University, Shenyang 110001, China.
    The ATM and Rad3-related (ATR) /checkpoint kinase 1 (Chk1) pathway plays a pivotal role in DNA damage sensor and modulating homologous recombination. Recently, emerging evidence demonstrated that Chk1 phosphorylation was associated with chemotherapy and radiotherapy resistance. In this study, we explored the effect of ATR/Chk1 pathway on regulating lapatinib sensitivity in human epidermal growth factor receptor-2 (HER2)-positive gastric cancer cell lines. Read More

    Extracellular matrix remodeling and TGF-β1/Smad signaling in diabetic colon mucosa.
    Cell Biol Int 2018 Apr 27;42(4):443-456. Epub 2017 Dec 27.
    Instituto Superior de Investigaciones Biológicas (INSIBIO) Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Tucumán (CONICET-UNT), Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina.
    Diabetes is associated with metabolic and functional alterations in the gut. Using an experimental model of streptozotocin (STZ)-induced diabetes in rodents, we analyzed the extracellular matrix (ECM) and TGF-β/Smad signaling in the colon mucosa. Male rats were divided into normal control, diabetic and insulin treated diabetic groups during 4 and 9 weeks. Read More

    Mechanisms of AMPK in the maintenance of ATP balance during energy metabolism.
    Cell Biol Int 2018 Apr 3;42(4):384-392. Epub 2018 Jan 3.
    Department of Gastroenterology, Research Institute of Digestive Diseases, The First Affiliated Hospital of Nanchang University, 17th Yongwaizheng St., Nanchang, Jiangxi, 330006, China.
    AMP-activated protein kinase (AMPK) is a conserved sensor of cellular energy change and is activated by increased AMP/ATP and/or ADP/ATP ratios. AMPK maintains the energy balance by decreasing the ATP-consuming processes such as transcription of synthetic fat genes and rRNA, the translation of ribosomal proteins, synthesis of cholesterol and fatty acid, while the metabolic pathways such as glucose and fatty transport, fatty acid oxidation, autophagy, mitochondrial synthesis and oxidative metabolism are increased to preserve ATP during energy deficiency. Recent advance has demonstrated that AMPK activity has a close association with the initiation and progression in various cancers. Read More

    Silencing of gelatinase expression delays myoblast differentiation in vitro.
    Cell Biol Int 2018 Mar 17;42(3):373-382. Epub 2017 Dec 17.
    Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, 1 Miecznikowa St., 02-096, Warsaw, Poland.
    Skeletal muscle growth and regeneration relies on the activation of muscle specific stem cells, that is, satellite cells. The activation and differentiation of satellite cells into myoblasts, as well as their migration, proliferation, and fusion of mononuclear myoblasts into a functional multi-nucleated muscle fiber, are associated with extracellular matrix (ECM) protein synthesis and degradation. The extracellular environment is dynamically adapting to the changes accompanying skeletal muscle growth or repair. Read More

    Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits.
    Cell Biol Int 2017 Nov 21. Epub 2017 Nov 21.
    Departamento de Microbiologia, Universidade de São Paulo, São Paulo, Brazil.
    Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. Read More

    PKG II effectively reversed EGF-induced protein expression alterations in human gastric cancer cell lines.
    Cell Biol Int 2018 Apr 14;42(4):435-442. Epub 2017 Dec 14.
    Medical School, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, P. R. China.
    Epidermal growth factor receptor (EGFR) plays an important role in gastric cancer (GC) progression. Our previous data demonstrated that type II cGMP-dependent protein kinase (PKG II) could block the EGF-EGFR axis as well as down-stream signaling pathways, for example, MAPK, PI3 K, and PLC in GC cells. However, the exact mechanisms of PKG II against cancer remain unclear. Read More

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