3,813 results match your criteria Cell Biology International [Journal]


Role of PLD-PKCζ signalling axis in p47phox phosphorylation for activation of NADPH oxidase by angiotensin II in pulmonary artery smooth muscle cells.

Cell Biol Int 2019 Apr 12. Epub 2019 Apr 12.

Department of Biochemistry and Biophysics, , University of Kalyani, Kalyan, West Bengal, India.

We sought to determine the mechanism by which angiotensin II (ANGII) stimulates NADPH oxidase-mediated O production in bovine pulmonary artery smooth muscle cells (BPASMCs). ANGII induced increase in phospholipase D (PLD) and NADPH oxidase activities were inhibited upon pretreatment of the cells with chemical and genetic inhibitors of PLD2, but not PLD1. Immunoblot study revealed that ANGII treatment of the cells markedly increases PKC-α, -δ, -ε and -ζ levels in the cell membrane. Read More

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http://dx.doi.org/10.1002/cbin.11145DOI Listing

Neurodegeneration, demyelination, and astrogliosis in rat spinal cord by chronic lead treatment.

Cell Biol Int 2019 Apr 12. Epub 2019 Apr 12.

Universidad Autónoma de Nuevo León (UANL), Facultad de Medicina, Departamento de Histología, Madero y Dr. Aguirre Pequeño, Mitras Centro, Monterrey, Nuevo León, México.

Early exposure to lead (Pb) has been associated with an elevated risk of developing neurodegenerative diseases. There is evidence that neuronal damage in chronic Pb exposure can be caused by the convergence of glial damage. Apoptosis may be a possible mechanism of Pb-induced cell death in the central nervous system. Read More

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http://dx.doi.org/10.1002/cbin.11147DOI Listing

Chrysophanol attenuated isoproterenol-induced cardiac hypertrophy through inhibiting JAK2/STAT3 signaling pathway.

Cell Biol Int 2019 Apr 12. Epub 2019 Apr 12.

Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Cardiac hypertrophy is a common pathological change found in various cardiovascular diseases. Although it has long been recognized as an important risk factor responsible for heart failure, there is still a lack of effective treatments in clinic. Chrysophanol is a natural compound with multiple biological activities and protective roles in cardiovascular system. Read More

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http://dx.doi.org/10.1002/cbin.11146DOI Listing
April 2019
2 Reads

ULTRASTRUCTURAL TRAITS OF APOPTOSIS.

Cell Biol Int 2019 Apr 10. Epub 2019 Apr 10.

Group of Cell Membrane Ultrastructure, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave, 194064, Petersburg, Russia.

This review summarizes original and literature data on changes in the ultrastructure of major cell organelles during apoptosis obtained by transmission electron microscopy. Organelles that make the most crucial contribution to the initiation of apoptosis: plasma membrane, mitochondria, proteasomes, Golgi apparatus, and endoplasmic reticulum, were of our prime attention. The nucleus and cytoskeleton that undergo essential changes, were considered as well. Read More

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http://dx.doi.org/10.1002/cbin.11148DOI Listing

The role of vasodilator-stimulated phosphoprotein in podocyte functioning.

Cell Biol Int 2019 Apr 10. Epub 2019 Apr 10.

Mossakowski Medical Research Centre of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, Poland.

Vasodilator-stimulated phosphoprotein (VASP) is a 39-kDa protein belonging to the Ena/VASP protein family, which is involved in adhesion, migration, cell-cell interaction, and regulation of pathways connected with actin cytoskeleton remodeling. VASP is activated through phosphorylation at Tyr39, Ser157, Ser239, Thr278, and Ser322 mainly by tyrosine kinase Abl, cAMP-dependent protein kinase, protein kinase G, AMP-activated protein kinase, and protein kinase D1, respectively. VASP activation, as a regulator of actin dynamics, may lead to impaired reorganization of the podocyte actin cytoskeleton not only by indirect interaction of VASP with actin but also by regulation of other signaling pathways. Read More

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http://dx.doi.org/10.1002/cbin.11149DOI Listing
April 2019
1 Read

On the interaction of ribosomal protein RPL22e with microtubules.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Institute of Protein Research of Russian Academy of Science, Pushchino, Moscow Region, Institutskaya str., Russia, 142290.

Microtubule (MT) protein preparations often contain components of the translation machinery, including ribosome proteins. To understand the biological meaning of it we studied the interaction of ribosomal protein RPL22e with the MT. We found that bacteria-expressed purified RPL22e-GFP-6His did co-sediment with brain tubulin MTs with 1. Read More

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http://dx.doi.org/10.1002/cbin.11141DOI Listing
April 2019
2 Reads

IgA1 isolated from Henoch-Schonlein Purpura children promotes proliferation of human mesangial cells in vitro.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Anatomy, , Anhui Medical University, Hefei, Anhui, China.

Previous studies show that the proliferation of human mesangial cells (HMCs) played a significant part in the pathogenesis of Henoch-Schönlein purpura nephritis (HSPN). The aim of this study was to explore the proliferation of HMCs induced by IgA1 isolated from sera of Henoch-Schönlein purpura (HSP) patients. HMCs were cultured in three different types of media, including IgA1 from patients with HSP (HSP IgA1 group), healthy children (healthy IgA1 group) and medium (control group). Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11142
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http://dx.doi.org/10.1002/cbin.11142DOI Listing
April 2019
2 Reads

MiR-377 inhibits wear particle-induced osteolysis via targeting RANKL.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Joint Surgery, The Third Affiliated Hospital, College of Medicine, Hebei University, Shijiazhuang, China.

Periprosthetic osteolysis caused by wear particles is the main factor that affected the long-term efficacy of artificial joint replacement, and macrophages played a vital role in the pathogenesis of periprosthetic osteolysis, while the potential mechanism underlying it was still unclear. To investigate the underlying role of miR-377 in wear particle-induced osteolysis (PIO), blood samples from patients undergoing arthroplasty were collected for analyzing the correlation between miR-377 expression and the clinicopathological parameters of PIO. Peripheral blood macrophages were obtained to compare miR-377 and receptor activator of NF-kappaB ligand (RANKL) expressions. Read More

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http://dx.doi.org/10.1002/cbin.11143DOI Listing
April 2019
1 Read
1.933 Impact Factor

The Absorption of Apolipoprotein E by Damaged Neurons Facilitates the Neuronal Repair.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Cardiology, The Central Hospital of Yongzhou.

Traumatic brain injury (TBI) is one of the common diseases diagnosed in the department of neurosurgery. Apolipoprotein E (apoE) can improve prognosis of TBI. In this study, we aimed to explore the effect of apoE in mechanically damaging neurons as well as its underlying molecular mechanism. Read More

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http://dx.doi.org/10.1002/cbin.11135DOI Listing

Effect of blue LED light and antioxidants potential in a somatic cell.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Japan.

Light is an indispensable part of routine laboratory works in which conventional light is generally used. Light-emitting diodes (LEDs) have come to replace the conventional light thus could be a potent target in biomedical studies. Since blue light is a major component of visible light wavelength, in this study, using a somatic cell from African green monkey kidney, we assessed the possible consequences of blue spectra of LED light in future animal experiments and proposed a potent mitigation against light induced damages. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11131
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http://dx.doi.org/10.1002/cbin.11131DOI Listing
April 2019
1 Read

Dexmedetomidine protects against high mobility group box 1-induced cellular injury by inhibiting pyroptosis.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Anesthesiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China.

Dexmedetomidine (DEX) is a widely used clinical anesthetic with a proven anti-inflammatory effects. Both high mobility group box 1 (HMGB1) and pyroptosis play an important role in the inflammatory response to infection and trauma. Thus far, there have been no studies published addressing the effect of DEX on HMGB1 and pyroptosis. Read More

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http://dx.doi.org/10.1002/cbin.11140DOI Listing
April 2019
1 Read

MiR-19b-3p regulates osteogenic differentiation of PDGFRα muscle cells by specifically targeting PTEN.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Orthopaedics and National Clinical Research Center for Geriatric Disorders, , Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.

Heterotopic ossification (HO) is a common disturbing complication of intra-articular fractures. Its prevention and treatment are still difficult as its pathogenesis is unclear. It was reported that PDGFRα muscle cells in skeletal muscle may participate in the formation of HO, however the specific mechanism is still unknown. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11133
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http://dx.doi.org/10.1002/cbin.11133DOI Listing
April 2019
4 Reads

Let-7b-5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

State Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases, Xinjiang Uygur Autonomous Region, Urumqi, PR China.

KIAA1377 has been found to be linked with lymph node metastasis in esophageal squamous cell carcinoma in our previous study; however, the regulation of KIAA1377 remains far from understood. Herein, to understand the regulation of KIAA1377 from the angle of miRNA-mRNA modulation in the setting of squamous cell carcinoma (SCC) cells, basal level of KIAA1377 was determined by quantitative real-time PCR (qRT-PCR) and western-blot in KYSE-150 and HeLa cells; Biological roles of KIAA1377 contributed in the proliferation, migration and invasion were evaluated using MTT, wound-healing and Transwell assays, respectively after KIAA1377 was knocked out mediated by CRISPR-Cas9 system. Bioinformatic prediction revealed that let-7b-5p was a putative miRNA regulating KIAA1377, which was ensuingly validated by luciferase reporter assay; after which, variation of KIAA1377 expression was further verified by qRT-PCR and western-blot. Read More

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http://dx.doi.org/10.1002/cbin.11136DOI Listing

Cell Death. A review of the major forms of Apoptosis, Necrosis and Autophagy.

Authors:
MarkSean D'Arcy

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Hertfordshire International College (HIC), Collage Lane, Hatfield, United Kingdom.

Cell death was once believed to be the result of one of two distinct processes, apoptosis (also known as programmed cell death) or necrosis (uncontrolled cell death); in recent years however several other forms of cell death have been discovered highlighting that a cell can die via a number of differing pathways. Apoptosis is characterised by a number of characteristic morphological changes in the structure of the cell, together with a number of enzyme-dependent biochemical processes. The result being the clearance of cells from the body, with minimal damage to surrounding tissues. Read More

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http://dx.doi.org/10.1002/cbin.11137DOI Listing

Possible Role of Differentially Expressing Novel Protein Markers (Ligatin and Fibulin-7) in Human Aqueous Humor and Trabecular Meshwork Tissue in Glaucoma Progression.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Biochemistry and Environmental Toxicology, Laboratory # 103, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.

The pathological mechanism underlying glaucoma has always been a complex aspect of this permanently blinding disease but proteomic studies have been helpful in elucidating it to a great extent in several studies. This study was designed to evaluate the expression and to get an idea about the function of two novel markers (Ligatin and Fibulin-7) identified in human aqueous humor (hAH) in relation to glaucomatous progression. A significant increase in the protein content of glaucomatous hAH compared to that of non-glaucomatous controls (NG-Ctrls) was observed. Read More

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http://dx.doi.org/10.1002/cbin.11138DOI Listing
April 2019
1.933 Impact Factor

JQ1, a BET-bromodomain inhibitor, inhibits human cancer growth and suppresses PD-L1 expression.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.

Most traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule-targeted drugs. PD-L1 is associated with the prognosis of several cancer types, and blockade of PD-1/PD-L1 signaling increases the amplitude of anti-tumor immunity. Read More

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http://dx.doi.org/10.1002/cbin.11139DOI Listing
April 2019
1 Read

The Caveolin-3 P104L mutation in LGMD-1C patients inhibits non-insulin-stimulated glucose metabolism and growth but promotes myocyte proliferation.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Physiology, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi, China, 2 Department of Endocrinology, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi, China.

The caveolin-3 (CAV3) protein is known to be specifically expressed in various myocytes, and skeletal muscle consumes most of the blood glucose as an energy source to maintain normal cell metabolism and function. The P104L mutation in the coding sequence of the human CAV3 gene leads to the autosomal dominant disease limb-girdle muscular dystrophy type 1C (LGMD-1C). We previously reported that C2C12 cells transiently transfected with the P104L CAV3 mutant exhibited decreased glucose uptake and glycogen synthesis after insulin stimulation. Read More

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http://dx.doi.org/10.1002/cbin.11144DOI Listing
April 2019
3 Reads

Role of autophagy in inhibiting the proliferation of A549 cells by type III interferon.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Institute of cell biology, Zhejiang University, Hangzhou, PR China.

Interferons (IFNs) have antiviral and anti-tumor effects. Type III interferon, as a member of the recently discovered interferon family, has been proved to inhibit tumor proliferation and promote the apoptosis of various tumor cells. However, whether type III IFN could inhibit the proliferation in lung cancer was not clear. Read More

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http://dx.doi.org/10.1002/cbin.11132DOI Listing

Knockdown of STAT3 targets a subpopulation of invasive melanoma stem-like cells.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Laboratory of Molecular Neurobiology, Neurobiology Center, The Nencki Institute of Experimental Biology, Warsaw, Poland.

Transcription factor STAT3 (Signal Transducer and Activator of Transcription 3) is constitutively activated in many cancers, including melanomas. Active, phosphorylated STAT3 contributes to tumor growth and formation of the immunosuppressive tumor microenvironment. Recent evidence suggests an important role of STAT3 in self-renewal of cancer stem-like cells (CSCs). Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11134
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http://dx.doi.org/10.1002/cbin.11134DOI Listing
April 2019
4 Reads

ADAMTS5 deficiency in mice does not affect cardiac function.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium.

The aggrecanase ADAMTS5 (A Disintegrin and Metalloproteinase with ThromboSpondin type 1 motifs, member 5) and the cleavage of its substrate versican have been implicated in the development of heart valves. Furthermore, ADAMTS5 deficiency was shown to protect against diet-induced obesity, a known risk factor for cardiovascular disease. Therefore, in this study we investigated a potential role of ADAMTS5 in cardiac function using ADAMTS5-deficient (Adamts5 ) mice and their wild-type (Adamts5 ) counterparts exposed to a standard-fat (SFD) or a high-fat diet (HFD). Read More

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http://dx.doi.org/10.1002/cbin.11130DOI Listing

Regulation of insulin resistance by targeting insulin-like growth factor 1 receptor (IGF-1R) with microRNA-122-5p in hepatic cells.

Cell Biol Int 2019 Apr 8. Epub 2019 Apr 8.

Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Insulin resistance (IR) is a common etiology of type 2 diabetes (T2D) defined by a state of decreased reactivity to insulin in multiple organs, such as liver. This study aims to investigate how microRNA-122-5p (miR-122) regulates the hepatic IR in vitro. We first found that miR-122 level was upregulated in the liver of rats fed with high-fat diet and injected with streptozotocin (T2D rats), while the expression level of insulin-like growth factor 1 receptor (IGF-1R), a potential target of miR-122, was downregulated in diabetic liver. Read More

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http://dx.doi.org/10.1002/cbin.11129DOI Listing
April 2019
3 Reads

Immunohistochemical Biomarkers and Distribution of Telocytes in ApoE Mice.

Cell Biol Int 2019 Mar 26. Epub 2019 Mar 26.

Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, 250014,, People's Republic of China.

Telocytes had been identified as a peculiar stromal cell type implicated in tissue homeostasis and the development and pathophysiology of diseases. Telocyte existed in most organs and tissues in humans and animals. However, few studies have examined telocytes in ApoE gene deficient mice. Read More

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http://dx.doi.org/10.1002/cbin.11128DOI Listing

Lipopolysaccharide upregulates the proliferation, migration, and odontoblastic differentiation of NG2 cells from human dental pulp in vitro.

Cell Biol Int 2019 Mar 7. Epub 2019 Mar 7.

Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, 200040, P. R. China.

NG2 cells have been proven to differentiate into odontoblasts in vivo, and their contribution to odontoblasts is significantly increased especially after tooth injury. However, their characteristics in vitro, especially under an inflammatory environment, are still not fully understood. Therefore, this study aimed to explore their proliferation, migration, and odontoblastic differentiation ability after treatmtent with lipopolysaccharide (LPS) in vitro. Read More

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http://doi.wiley.com/10.1002/cbin.11127
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http://dx.doi.org/10.1002/cbin.11127DOI Listing
March 2019
6 Reads

Bone mesenchymal stem cell-conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway.

Cell Biol Int 2019 Mar 6. Epub 2019 Mar 6.

Department of Spine Surgery, The First Affiliated Hospital of Anhui Medical University.

Numerous studies have demonstrated the therapeutic effect of bone mesenchymal stem cells on Spinal cord injury (SCI), especially on neural stem cells (NSCs). However, the predominant mechanisms of bone mesenchymal stem cell (BMSCs) are unclear. Recently, some researchers have found that paracrine signaling plays a key role in the therapeutic capacity of BMSCs and emphasized that the protective effect of BMSCs may be due to paracrine factors. Read More

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http://dx.doi.org/10.1002/cbin.11126DOI Listing
March 2019
1 Read

TNF-α mediated MEK-ERK signaling in invasion with putative network involving NF-κB and STAT-6: a new perspective in glioma.

Cell Biol Int 2019 Mar 6. Epub 2019 Mar 6.

Department of Neuroimaging and Intervention Radiology, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru 560029, India.

Glioblastoma is the most common malignant primary brain tumor with poor prognosis. Invasion involves proinflammatory cytokines and major signaling hubs. Tumor necrosis factor (TNF)-α acts as a master switch in establishing an intricate link between inflammation and cancer. Read More

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http://dx.doi.org/10.1002/cbin.11125DOI Listing
March 2019
1 Read

Oxidized low-density lipoprotein promotes vascular endothelial cell dysfunction by stimulating miR-496 expression and inhibiting the Hippo pathway effector YAP.

Cell Biol Int 2019 Feb 27. Epub 2019 Feb 27.

Xuhui Central Hospital, Shanghai Clinical Research Center, China Academy of Sciences, Shanghai, 200031, China.

Oxidized low-density lipoprotein (ox-LDL) can damage vascular endothelial cells and cause atherosclerosis, but its epigenetic regulatory mechanism has not been fully elucidated. We show that ox-LDL induced significant apoptosis and loss of function in human umbilical vascular endothelial cells (HUVECs). At the same time, ox-LDL significantly decreased the expression of Hippo-YAP/ZAP (Yes-associated protein/YLP motif-containing 1) pathway proteins as compared to that of the control. Read More

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http://dx.doi.org/10.1002/cbin.11120DOI Listing
February 2019
1 Read
1.933 Impact Factor

The influence of serum-supplemented culture media in a transwell migration assay.

Cell Biol Int 2019 Feb 27. Epub 2019 Feb 27.

Neuroinflammation Group, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia.

This study determines the effect of serum-supplemented culture media on BV2 microglia migration in a transwell assay. At 24 hours, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate towards the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Read More

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http://dx.doi.org/10.1002/cbin.11122DOI Listing
February 2019
1 Read

Nanocomposite scaffold seeded with mesenchymal stem cells for bone repair.

Cell Biol Int 2019 Feb 27. Epub 2019 Feb 27.

Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.

Mechanical property of bone tissue scaffolds is one of the most important aspects in bone tissue engineering that has remained problematic. In our previous study, we fabricated a 3D scaffold from nano-hydroxyapatite/gelatin (nHA/Gel) and investigated its efficiency in promoting bone regeneration both in vitro and in vivo. In the present study, the effect of adding silicon carbide (SiC) on mechanical and biological behaviors of the nHA/Gel/SiC and bone regeneration in vivo were determined. Read More

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http://dx.doi.org/10.1002/cbin.11124DOI Listing
February 2019
1.933 Impact Factor

Progesterone and fluoxetine treatments of postpartum depressive-like behavior in rat model.

Cell Biol Int 2019 Feb 27. Epub 2019 Feb 27.

Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Research studies have indicated that alterations in plasma progesterone levels might be associated with the hippocampal synaptic plasticity of postpartum depressive-like behavior. Herein, we assess both progesterone and fluoxetine effects in adult female Sprague-Dawley rats with postpartum depressive-like behavior. Depressive-like behavior of postpartum rats was established using chronic ultra-mild stress (CUMS) method for 1 week from gestation day 15. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11123
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http://dx.doi.org/10.1002/cbin.11123DOI Listing
February 2019
3 Reads
1.933 Impact Factor

The Induction of Ferroptosis by Impairing STAT3/Nrf2/GPx4 Signaling Enhances the Sensitivity of Osteosarcoma Cells to Cisplatin.

Cell Biol Int 2019 Feb 27. Epub 2019 Feb 27.

Orthopedic Departments, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710054, China.

Recent studies have indicated that promoting ferroptosis is a promising approach to attenuate drug resistance of cancer cells. Hence, this study aimed to induce ferroptosis in osteosarcoma cells, thereby increasing sensitivity to cisplatin. Osteosarcoma cells MG63 and Saos-2 were incubated with increasing doses of cisplatin to generate cisplatin-resistant strains, MG63/DDP and Saos-2/DDP. Read More

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http://dx.doi.org/10.1002/cbin.11121DOI Listing
February 2019

Cellular responses to ionizing radiation change quickly over time during early development in zebrafish.

Cell Biol Int 2019 Feb 21. Epub 2019 Feb 21.

Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine (RIRBM), Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 754-8553, Japan.

Animal cells constantly receive information about and respond to environmental factors, including ionizing radiation. Although it is crucial for a cell to repair radiation-induced DNA damage to ensure survival, cellular responses to radiation exposure during early embryonic development remain unclear. In this study, we analyzed the effects of ionizing radiation in zebrafish embryos and found that radiation-induced γH2AX foci formation and cell cycle arrest did not occur until the gastrula stage, despite the presence of major DNA repair-related gene transcripts, passed on as maternal factors. Read More

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http://dx.doi.org/10.1002/cbin.11117DOI Listing
February 2019

Adipose tissue -derived mesenchymal stem cells and keratinocytes co-culture on Gelatin/Chitosan/β- Glycerol Phosphate Nanoscaffold in skin regeneration.

Cell Biol Int 2019 Feb 21. Epub 2019 Feb 21.

Stem Cell and Regenerative Medicine Research Group, Academic Center for Education, Culture and Research(ACECR), Razavi Khorasan Branch, Mashhad, Iran.

Using cell-based engineered skin is an emerging strategy for treating difficult-to-heal wounds To date, much endeavor has been devoted to the -fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound. The aim of this research was to assess the impact of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and keratinocytes on Gelatin/Chitosan/β-Glycerol Phosphate (GCGP) nanoscaffold in full-thickness excisional skin wound healing of rats. . Read More

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http://dx.doi.org/10.1002/cbin.11119DOI Listing
February 2019
1 Read
1.933 Impact Factor

A role of GCC88 in the retrograde transport of CI-M6PR and the maintenance of lysosomal activity.

Cell Biol Int 2019 Feb 21. Epub 2019 Feb 21.

School of Biomedical Sciences, Faculty of Medicine, The University of Queensland, Brisbane, Queensland, Australia.

GCC88 is a golgin coiled-coil protein at the trans-Golgi (TGN) that functions as a tethering factor for the endosome derived retrograde transport vesicles. Here, we demonstrate that GCC88 is required for the endosome-to-TGN retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-M6PR). The knockout of GCC88 perturbs the retrieval of CI-M6PR and decreases its cellular level at the steady state, which causes the improper processing of newly synthesized cathepsin-D, a lysosomal hydrolase dependent on CI-M6PR for its delivery to lysosomes. Read More

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http://dx.doi.org/10.1002/cbin.11118DOI Listing
February 2019

Role of allograft inflammatory factor-1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, People's Republic of China.

Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated with inflammation in the cells other than macrophages, such as endothelial cells and vascular smooth muscle cells. To clarify the molecular mechanisms of AIF-1-modulated pathological changes in the peritoneum during PD, we first detected the AIF-1 expression in peritoneal tissues from PD mice. Read More

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http://dx.doi.org/10.1002/cbin.11115DOI Listing
February 2019
2 Reads

Expression of proteins related to thyroid hormone function in the uterus is down-regulated at the day of implantation in hypothyroid pregnant rats.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Department of Obstetrics & Gynecology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Hypothyroidism has been linked to infertility, but the mechanisms underlying infertility-related hypothyroidism have yet to be fully elucidated. Therefore, in this study, effects of hypothyroidism on expression of the proteins related to thyroid hormone function in the uterus, which were thought to play a role implantation, including thyroid hormone receptor (TR), thyroid stimulating hormone receptor (TSHR), retinoic acid receptor (RAR) and extracellular kinase (ERK) were identified. Pregnant female rats were rendered hypothyroid by giving methimazole (MMI), orally. Read More

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http://dx.doi.org/10.1002/cbin.11114DOI Listing
February 2019
2 Reads

Periostin plays role in force-induced stem cell potential by periodontal ligament stem cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Mechanical stimuli have been shown to play an important role in directing stem cell fate and maintenance of tissue homeostasis. One of the functions of the mechanoresponsive tissue periodontal ligament (PDL) is to withstand the functional forces within the oral cavity. Periodontal ligament stem cells (PDLSCs) derived from periodontal tissue have been demonstrated to be able to respond directly to mechanical forces. Read More

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http://dx.doi.org/10.1002/cbin.11116DOI Listing
February 2019

Insulin-like growth factor-I promotes hepatic differentiation of human adipose tissue-derived stem cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

There are numerous studies which provide support for the use of human adipose tissue-derived stem cells (hASCs) to generate hepatocyte-like cells. However, the produced cells exhibit only a certain level of differentiation, mainly due to inefficient induction conditions. Therefore, based on the important role of insulin-like growth factor (IGF-I) in hepatic function and development, in the current study we evaluated the differentiation efficacy of the mentioned factor to induce hASCs into functional hepatocyte-like cells. Read More

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http://dx.doi.org/10.1002/cbin.11113DOI Listing
February 2019
1.933 Impact Factor

The use of apocynin inhibits osteoclastogenesis.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Institute of Biomedical Science, Federal University of Uberlandia, 2B, Room, 211, Campus Umuarama, Uberlandia, Brazil.

Reactive oxygen species (ROS) are produced by NADPH oxidase (NOX), an enzyme that reduces oxygen by using NADPH as a substrate. Apocynin (APO) is a catechol that is used as a NOX inhibitor, and N-acetyl-cysteine ​​(NAC) can reduce intracellular ROS levels. In this work, the effect of APO and NAC on osteoclast formation were evaluated. Read More

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http://dx.doi.org/10.1002/cbin.11110DOI Listing
February 2019
1 Read

Spinal cord tissue affects sprouting from aortic fragments in ex vivo co-culture.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

National Research Center Kurchatov Institute, Moscow, Russian Federation.

It is a well-known fact, that there is a close interconnection between vascular and neural structures in both embryonic development and postnatal life. Different models have been employed to dissect the mechanisms of these interactions, ranging from in vitro systems (e.g. Read More

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http://dx.doi.org/10.1002/cbin.11112DOI Listing
February 2019
7 Reads

HIV-1 infection modulates IL-24 expression which contributes to cell apoptosis in vitro.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Disciplina de Infectologia, Laboratório de Retrovirologia, Universidade Federal de São Paulo, São Paulo, Brazil.

Although interleukin-24 (IL-24) has been extensively explored in the immunopathologies of autoimmune diseases, neoplasms, and infections, its role in HIV-1 infection has not been thoroughly elucidated to date. Therefore, the objective of this study was to evaluate the gene and protein expressions of IL-24 at the initial moments of HIV infection in PBMCs. Due to the pro-apoptotic role of IL-24, we evaluated the protein expression of caspase-3, as well as Annexin V/Propidium Iodide flow cytometry and phosphorylation of ERK, which may induce an apoptotic signal block when phosphorylated. Read More

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http://dx.doi.org/10.1002/cbin.11111DOI Listing
February 2019
2 Reads

The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture.

Cell Biol Int 2019 Feb 6. Epub 2019 Feb 6.

Department of Regenerative Medicine, Cell and Tissue Bank, Chair of Urology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Poland.

Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self-renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Read More

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http://dx.doi.org/10.1002/cbin.11109DOI Listing
February 2019

IL-7 enhances the differentiation of adipose-derived stem cells toward lymphatic endothelial cells through AKT signaling.

Cell Biol Int 2019 Apr 6;43(4):394-401. Epub 2019 Feb 6.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, P. R. China.

Our study was designed to investigate the effects of IL-7 during the differentiation process of adipose-derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs). IL-7 was added to the traditional induced medium, which was called the IL-7 (+) group, while the group that used traditional induced medium was called the IL-7 (-) group. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups. Read More

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http://dx.doi.org/10.1002/cbin.11093DOI Listing
April 2019
2 Reads

Polypeptides secreted from the columnar vesicles of the sea anemone Bunodosoma cangicum and their in vivo effects on Caenorhabditis elegans.

Cell Biol Int 2019 Apr 19;43(4):429-436. Epub 2019 Feb 19.

Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande-FURG, Rio Grande, Rio Grande do Sul, 96203-900, Brazil.

In this study we provide new evidence that the columnar vesicles of the sea anemone Bunodosoma cangicum are toxic in vivo and contain at least two active polypeptides, a neurotoxic and an apoptosis inducing polypeptide. Here we show that it is also an effective inducer of apoptosis in vivo in the nematode Caenorhabditis elegans. In addition, the anemone peptides rapidly paralyze C. Read More

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http://dx.doi.org/10.1002/cbin.11107DOI Listing
April 2019
3 Reads

Improvements in chemical carriers of proteins and peptides.

Authors:
Azam Bolhassani

Cell Biol Int 2019 Apr 19;43(4):437-452. Epub 2019 Feb 19.

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

The successful intracellular delivery of biologically active proteins and peptides plays an important role for therapeutic applications. Indeed, protein/peptide delivery could overcome some problems of gene therapy, for example, controlling the expression levels and the integration of transgene into the host cell genome. Thus, protein/peptide drug delivery showed a promising and safe approach for treatment of cancer and infectious diseases. Read More

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http://dx.doi.org/10.1002/cbin.11108DOI Listing
April 2019
2 Reads

Silencing of MEG3 inhibited ox-LDL-induced inflammation and apoptosis in macrophages via modulation of the MEG3/miR-204/CDKN2A regulatory axis.

Cell Biol Int 2019 Apr 22;43(4):409-420. Epub 2019 Feb 22.

Neurovascular Surgery, The First Bethune Hospital of Jilin University, 71 Xinmin Street, Chaoyang District, Changchun City, 130012, Jilin Province, People's Republic of China.

Atherosclerosis (AS) is one of the most common chronic inflammatory diseases and a major cause of morbidity and mortality. However, the underlying molecular mechanisms of the progression of AS are still largely unknown. Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) play important roles in a variety of biological processes and the physiological and pathological progression of human diseases. Read More

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http://dx.doi.org/10.1002/cbin.11105DOI Listing
April 2019
4 Reads

Functional prediction and characterization of Dip2 gene in mice.

Cell Biol Int 2019 Apr 19;43(4):421-428. Epub 2019 Feb 19.

Key Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130021, China.

Disconnected interacting protein 2 (DIP2) is a highly conserved protein family among invertebrates and vertebrates, but its function remains unclear. In this paper, we summarized the conservation of gene sequences and protein domains of DIP2 family members and predicted that they may have a similar functional role in acetyl-coenzyme A (acetyl-CoA) synthesis. We then used the most characterized member, disconnected interacting protein 2 homolog A (DIP2A), for further study. Read More

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http://dx.doi.org/10.1002/cbin.11106DOI Listing
April 2019
15 Reads

MiR-9 promotes multiple myeloma progression by regulating TRIM56/NF-κB pathway.

Cell Biol Int 2019 Jan 14. Epub 2019 Jan 14.

Department of Hematology, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, 710061, China.

MiR-9 has been reported to play a pivotal role in multiple human cancers through acting as an oncogene or tumor suppressor. In this study, we explored the possible role and molecular mechanism of miR-9 in multiple myeloma (MM). MiR-9 expression was examined by qRT-PCR assay. Read More

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http://doi.wiley.com/10.1002/cbin.11104
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http://dx.doi.org/10.1002/cbin.11104DOI Listing
January 2019
9 Reads

Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus-affected gestations: A focus on myogenic and neural differentiations.

Cell Biol Int 2019 Mar 28;43(3):299-312. Epub 2019 Jan 28.

Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, Vilnius, LT-10257, Lithuania.

Amniotic fluid-derived mesenchymal stem cells (AF-MSCs) are autologous to the fetus and represent a potential alternative source for the regenerative medicine and treatment of perinatal disorders. To date, AF-MSCs differentiation capacity to non-mesodermal lineages and epigenetic regulation are still poorly characterized. The present study investigated the differentiation potential of AF-MSCs toward neural-like cells in comparison to the mesodermal myogenic lineage and assessed epigenetic factors involved in tissue-specific differentiation. Read More

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http://dx.doi.org/10.1002/cbin.11099DOI Listing
March 2019
4 Reads

The Hippo-YAP Pathway Regulates the Proliferation of Alveolar Epithelial Progenitors after Acute Lung Injury.

Cell Biol Int 2019 Jan 11. Epub 2019 Jan 11.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Military Medical University, Chongqing 400042, China.

Regeneration of pulmonary epithelial cells plays an important role in the recovery of acute lung injury (ALI) which is defined by pulmonary epithelial cell death. However, the mechanism of the regenerative capacity of alveolar epithelial cells is unknown. Using a lung injury mouse model induced by hemorrhagic shock and LPS, a protein mass spectrometry-based high-throughput screening and linage tracing technology to mark alveolar epithelial type 2 cells (AEC2s), we analyzed the mechanism of alveolar epithelial cells proliferation. Read More

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http://doi.wiley.com/10.1002/cbin.11098
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http://dx.doi.org/10.1002/cbin.11098DOI Listing
January 2019
13 Reads

ER stress differentially affects pro-inflammatory changes induced by mitochondrial dysfunction in the human monocytic leukemia cell line, THP-1.

Cell Biol Int 2019 Mar 29;43(3):313-322. Epub 2019 Jan 29.

School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, Republic of Korea.

The functional and physical interaction between mitochondria and the endoplasmic reticulum (ER) has been the subject of intense study. To test the effect of this interaction on macrophage inflammatory activation, the human macrophage-like monocytic leukemia cell line THP-1 was treated with oligomycin, rotenone, or sodium azide, which induce mitochondrial dysfunction (MD) by blocking the electron transport chain (ETC). MD induced by these agents triggered activation of various sensors and markers of ER stress. Read More

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http://doi.wiley.com/10.1002/cbin.11103
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http://dx.doi.org/10.1002/cbin.11103DOI Listing
March 2019
7 Reads