3,695 results match your criteria Cell Biology International [Journal]


Transforming growth factor-β 1 increases C-C chemokine ligand 11 production in interleukin 4-stimulated human periodontal ligament cells.

Cell Biol Int 2018 Jul 11. Epub 2018 Jul 11.

Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.

Transforming growth factor (TGF)-β1 is a multifunctional cytokine, which can control certain functions of various kinds of cells. However, it is unclear whether TGF-β1 affects T-cell migration in periodontal lesions. The aim of this study was to examine the effects of TGF-β1 on the production of C-C chemokine ligand (CCL)11, which is a T-helper 2-type chemokine, in human periodontal ligament cells (HPDLC). Read More

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Dynamic changes in chromatin and microtubules at the first cell cycle in SCNT or IVF goat embryos.

Cell Biol Int 2018 Jul 11. Epub 2018 Jul 11.

College of Life Sciences, Nankai University, Tianjin 300071, China.

We investigated the dynamic changes in chromatin and microtubules at the first cell cycle in goat somatic cell nuclear transfer (SCNT)-derived and in vitro fertilization (IVF) derived embryos. Stage-dependent and characteristic changes to chromatin and microtubules occurred in SCNT-derived embryos at different times after activation. About half donor nuclei underwent premature chromosome condensation (PCC) at 1 h post activation, and furtherly reached telophase at 2 h after activation. Read More

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Aggravated Intestinal Apoptosis by ClC-3 Deletion is Lethal to Mice Endotoxemia.

Cell Biol Int 2018 Jul 4. Epub 2018 Jul 4.

School of Medical Technology, Xuzhou Medical University, 209 Tongshan Rd, Xuzhou, Jiangsu 221004, P.R. China.

Our previous study found that ClC-3 chloride channel functioned differently in the vascular and intestinal inflammation, the loss of ClC-3 reduced vascular inflammation but exacerbated intestinal inflammation. To furtherly clarify the role of ClC-3 chloride channels in systemic inflammation, we used LPS-induced endotoxemia model to investigate the response of wild-type and ClC-3 knockout mice to systemic inflammation. The results showed that in the LPS-induced endotoxemia model, the mortality of mice with ClC-3 deletion was significantly higher than that of wild-type mice. Read More

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July 2018
1 Read

Down-regulation of miR-26 plays essential roles in TGFβ-induced EMT.

Cell Biol Int 2018 Jul 4. Epub 2018 Jul 4.

Department of Cardio-Thoracic Surgery, Number 455 Hospital of The Chinese People's Liberation Army, Shanghai 200052, China.

Epithelial mesenchymal transformation (EMT) plays fundamental roles in tumor metastasis. MiRNAs function as powerful regulators of EMT. The function and mechanism of miRNAs in EMT of lung cancers remains to be elucidated. Read More

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Bcl6 knockdown aggravates hypoxia injury in cardiomyocytes via the P38 pathway.

Cell Biol Int 2018 Jul 4. Epub 2018 Jul 4.

Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China.

B-cell lymphoma 6 (Bcl6) functions as a sequence-specific transcriptional repressor and negative regulator of many signaling proteins. The effects of Bcl6 on cardiomyocyte injury are not clear. This study was designed to determine whether Bcl6 affects hypoxia-induced cardiomyocyte injury and, if so, to identify the underlying mechanism. Read More

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Suppressed Expression of Cbl-b by NF-κB Mediates Icotinib Resistance in EGFR-mutant Non-Small-Cell Lung Cancer.

Cell Biol Int 2018 Jul 4. Epub 2018 Jul 4.

Department of Medical Oncology, The First Hospital of China Medical University, No.155, North Nanjing Street, Heping District Shenyang 110001, China.

Although epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) could greatly improve the prognosis of NSCLC patients harboring activating EGFR mutations, drug resistance still remains a major obstacle to successful treatment. Our previous study found that the EGFR-TKI icotinib could upregulate the expression of Casitas-B-lineage lymphoma protein-B (Cbl-b), an E3 ubiquitin ligase. In the present study, we aimed to clarify the potential role of Cbl-b in the resistance to icotinib, and the underlying mechanisms using EGFR-mutant cell lines. Read More

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July 2018
5 Reads

Inhibition of CXCR4 regulates epithelial mesenchymal transition of NSCLC via the Hippo-YAP signaling pathway.

Cell Biol Int 2018 Jul 4. Epub 2018 Jul 4.

Department of Cardiothoracic Surgery, Lishui Central Hospital, Lishui Hospital of Zhejiang University, 323000, Zhejiang Province, P.R. China.

CXCR4 has been shown to play a key role in the metastasis of non-small cell lung cancer (NSCLC). And CXCR may be associated with the Hippo - Yes kinase-associated protein (YAP) pathway, thus involving in the occurrence and progression of NSCLC. This study aims to investigate the effect of CXCR4 inhibition on epithelial-mesenchymal transition (EMT), invasion and migration of NSCLC cells via the Hippo-YAP pathway. Read More

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July 2018
2 Reads

MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/β-catenin pathway.

Cell Biol Int 2018 Jul 4. Epub 2018 Jul 4.

Department of Dermatology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.

MicroRNAs (miRNAs) participate in the development and progression of melanoma. However, while dysregulation of microRNA-378 (miR-378) has been seen in various cancer types, its clinical importance and function in melanoma is poorly elucidated. In this work, miR-378 expression in melanoma and in adjacent non-cancerous tissue was evaluated with quantitative real time polymerase chain reaction (qRT-PCR). Read More

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July 2018
1 Read

MicroRNA-34a-5p inhibits liver fibrosis by regulating TGF-β1/Smad3 pathway in hepatic stellate cells.

Cell Biol Int 2018 Jun 29. Epub 2018 Jun 29.

Department of infectious diseases, YiWu Central Hospita, Zhejiang 322000, China.

Liver fibrosis is a major cause of morbidity and mortality worldwide, and the outcome of various chronic liver diseases. Recent studies suggest that aberrant expression of miR-34 is involved in the progression of various liver diseases including hepatocellular carcinoma (HCC). However, it is still poorly understood whether miR-34 mediates the pathogenesis of liver fibrosis. Read More

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June 2018
2 Reads

β-Elemene increases the sensitivity of gastric cancer cells to TRAIL by promoting the formation of DISC in lipid rafts.

Cell Biol Int 2018 Jun 29. Epub 2018 Jun 29.

Department of Medical Oncology, the First Hospital of China Medical University, Shenyang 110001, China.

β-Elemene, an anti-cancer drug extracted from traditional Chinese medicinal herb, showed anti-tumor effects on gastric cancer cells. Our previous studies reported gastric cancer cells are insensitive to TRAIL. However, whether β-elemene could enhance anti-cancer effects of TRAIL on gastric cancer cells is unknown. Read More

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June 2018
1 Read

LncRNA Gm5091 alleviates alcoholic hepatic fibrosis by sponging miR-27b/23b/24 in mice.

Cell Biol Int 2018 Jun 23. Epub 2018 Jun 23.

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.

The regulatory roles of lncRNAs in the development of alcoholic hepatic fibrosis (AHF) have not been revealed. Here, we found lncRNA Gm5091 was being downregulated in mouse hepatic stellate cells (HSCs) during AHF. Then, Gm5091 was respectively overexpressed and knocked down in alcohol-treated primary HSCs. Read More

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PKG II inhibits PDGF-BB triggered biological activities by phosphorylating PDGFRβ in gastric cancer cells.

Cell Biol Int 2018 Jun 23. Epub 2018 Jun 23.

School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China.

Previous studies revealed that type II cGMP-dependent protein kinase G (PKG II) could inhibit the activation of epidermal growth factor receptor (EGFR) which is a widely investigated RTK. PDGFR belongs to family of receptor tyrosine kinases (RTKs) too. However, the effect of PKG II on PDGFR activation is not clear yet. Read More

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Iliac and mandible osteoblasts exhibit varied responses to LMHF vibration.

Cell Biol Int 2018 Jun 19. Epub 2018 Jun 19.

Orthodontic Section, Faculty of Dentistry, Department of Preventive Dentistry, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.

The facial and long bones have distinct developmental origins, structures, and cellular compositions. This study aimed to compare the in vitro responses of human mandible and long bone osteoblasts to low-magnitude, high-frequency (LMHF) mechanical vibration in terms of expression of mediators of bone remodeling. Osteoblast-like cell cultures were prepared from iliac crest and mandibular bone specimens from three individuals and cultured in osteogenic induction media. Read More

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Rapamycin improves insulin resistance and hepatic steatosis in type 2 diabetes rats through activation of autophagy.

Cell Biol Int 2018 Jun 16. Epub 2018 Jun 16.

Department of Endocrinology, Anhui Provincial Hospital, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, No. 17 Lujiang Road, Hefei, Anhui 230001, China.

Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the model of T2DM rats was established by feeding the animals with a high-fat diet (HFD) and intraperitoneal injection of streptozotocin (STZ). Read More

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June 2018
3 Reads

CoCl , a mimic of hypoxia, enhances bone marrow mesenchymal stem cells migration and osteogenic differentiation via STAT3 signaling pathway.

Cell Biol Int 2018 Jun 16. Epub 2018 Jun 16.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine, Ministry of Education, School and Hospital of Stoma-tology, Wuhan University, 237 Luoyu Road, Wuhan 430079, PR China.

Mesenchymal stem cells homing and migration is a crucial step during bone fracture healing. Hypoxic environment in fracture site induces bone marrow mesenchymal stem cells (BMSCs) migration, but its mechanism remains unclear. Our previous study and studies by other groups have reported the involvement of signal transducer and activator of transcription 3 (STAT3) pathway in cell migration. Read More

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Serpin A1 and the modulation of type I collagen turnover: Effect of the C-terminal peptide 409-418 (SA1-III) in human dermal fibroblasts.

Cell Biol Int 2018 Jun 16. Epub 2018 Jun 16.

NEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Viale G. Pieraccini 6, Florence, 50134, Italy.

The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III increases extracellular type I collagen levels, to compare its activities range with that of the originator molecule Serpin A1, and to evaluate its efficacy in primary cultures from adult and aged human subjects. Read More

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June 2018
2 Reads

Lipopolysaccharide induced the proliferation of mouse lung fibroblasts by suppressing FoxO3a/p27 pathway.

Cell Biol Int 2018 Jun 16. Epub 2018 Jun 16.

Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Aberrant aggregation and activation of lung fibroblasts is a key process in pulmonary fibrosis, but the underlying mechanism remains enigmatic. Forkhead Box O3a (FoxO3a) is considered to be an important transcription factor that could regulate both cell cycle and cell viability. To investigate the role of FoxO3a on LPS-induced lung fibroblast proliferation, we transfected FoxO3a-SiRNA or FoxO3a-OE lentivirus into cultured mouse lung fibroblasts to knockdown or overexpress FoxO3a and pretreated mouse lung fibroblasts with gefitinib to enhance FoxO3a activity. Read More

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Down-regulation of exosomal miR-106b-5p derived from cholesteatoma perimatrix fibroblasts promotes angiogenesis in endothelial cells by overexpression of Angiopoietin 2.

Cell Biol Int 2018 Jun 15. Epub 2018 Jun 15.

Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xi'an Jiaotong University, No.157, Xi Wu Lu, Xi'an 710004, Shaanxi Province, China.

Human cholesteatoma perimatrix fibroblasts (hCPFs) can stimulate the endothelial cells of nearby microvessels to proliferate and migrate in a paracrine manner. Exosomes, secreted from various cell types, are one of the most important paracrine factors and play critical roles in intercellular communication. However, whether exosomes derived from human cholesteatoma perimatrix fibroblasts (hCPFs-Exo) can promote angiogenesis has not been reported. Read More

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June 2018
1 Read

Genome-wide identification, phylogenetic classification, and exon-intron structure characterization of the tubulin and actin genes in flax (Linum usitatissimum).

Cell Biol Int 2018 Jun 8. Epub 2018 Jun 8.

Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, Osipovskogo St. 2a, 04123, Ukraine.

Flax (Linum usitatissimum L.) is a valuable food and fiber crop cultivated for its quality fiber and seed oil. α-, β-, γ-tubulins and actins are the main structural proteins of the cytoskeleton. Read More

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Tyrosine kinase inhibitor-induced IL-6/STAT3 activation decreases sensitivity of EGFR-mutant non-small cell lung cancer to icotinib.

Cell Biol Int 2018 Jun 8. Epub 2018 Jun 8.

Department of Medical Oncology, Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning Province, The First Hospital of China Medical University, No. 155, North Nanjing Street, Heping District, Shenyang 110001, China.

Tyrosine kinase Inhibitors (TKIs) of epidermal growth factor receptor (EGFR) has considerably benefited for non-small cell lung carcinomas (NSCLC) harbor mutations in EGFR. However, the factors attenuating EGFR-TKI efficiency are obstacles to inhibit the proliferation of EGFR-mutant NSCLC cells successfully. Clarifying the insensitivity mechanisms of EGFR-TKI would help to develop new treatment strategy. Read More

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June 2018
5 Reads

MicroRNA-1 overexpression increases chemosensitivity of non-small cell lung cancer cells by inhibiting autophagy related 3-mediated autophagy.

Cell Biol Int 2018 May 30. Epub 2018 May 30.

Emergency Internal Medicine, Xiyuan Hospital of Chinese Academy of Chinese Medical Sciences, Beijing, 100091, China.

Non-small cell lung cancer (NSCLC) is a major type of lung cancer. Drug resistance is a enormous obstacle for cancer treatment. Copious microRNAs (miRNAs) have been demonstrated to be implicated in drug resistance in NSCLC. Read More

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Dexmedetomidine protects hepatic cells against oxygen-glucose deprivation/reperfusion injury via lncRNA CCAT1.

Cell Biol Int 2018 May 30. Epub 2018 May 30.

Department of Hepatobillary Surgery, The First Affiliated Hospital of Chongqing Medical University, No.1 Youyi Road, Yuzhong District, Chongqing, P.R. China.

Dexmedetomidine (Dex) protects different cell types during hypoxia or ischemia-reperfusion injury by inhibiting cell apoptosis. However, the underlying mechanism and its impact on hepatic ischemia reperfusion injury are still not known. In this study, we established a model of oxygen-glucose deprivation/reperfusion (OGD/R) injury in hepatocyte HL7702 cells, and studied the impact of Dex on cell proliferation, apoptosis, and cell cycle during OGD/R. Read More

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May 2018
1 Read

The suppressive effect of soluble Klotho on fibroblastic growth factor 23 synthesis in UMR-106 osteoblast-like cells.

Cell Biol Int 2018 May 30. Epub 2018 May 30.

Department of Nephrology, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.

Fibroblastic growth factor 23 (FGF23) is a hormone secreted primarily by bone. FGF23 is elevated in the serum of chronic kidney disease (CKD) patients, but the exact mechanism is not well known. Klotho is identified as an aging suppressor, which is mainly expressed in the kidney, and the level of soluble Klotho is negatively associated with FGF23 in CKD. Read More

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Ca -depended signaling pathways regulate self-renewal and pluripotency of stem cells.

Cell Biol Int 2018 May 31. Epub 2018 May 31.

Institute of Cytology RAS, Saint-Petersburg 194064, Russia.

Ca -mediated signaling is widely spread in nature and plays critical role in the individual development of various organisms ranging from microorganisms to mammals. In vertebrates, Ca is involved in important developmental events: fertilization, body plan establishment, and organogenesis. The two later events are defined by embryonic stem cells (ESCs). Read More

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May 2018
1 Read

Endothelial progenitor cells from aged subjects display decreased expression of sirtuin 1, angiogenic functions, and increased senescence.

Cell Biol Int 2018 May 31. Epub 2018 May 31.

Schoolof Biotechnology, Gautam Buddha University, Greater Noida, India.

Studies have demonstrated that aging is associated with a substantial decline in numbers and angiogenic activity of endothelial progenitor cells (EPCs). In view of senescence being an important regulator of age-related cell survival and function, in the current study, we correlated EPCs numbers and functions with their senescence status and mechanisms in young and elderly subjects. Healthy young subjects (n = 30, below 60 y) and old subjects (n = 30, equal to or above 60 y) participated in the study. Read More

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Down-regulation of CD19 expression inhibits proliferation, adhesion, migration and invasion and promotes apoptosis and the efficacy of chemotherapeutic agents and imatinib in SUP-B15 cells.

Cell Biol Int 2018 May 29. Epub 2018 May 29.

Division of Hematology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, PR China.

The survival rate of childhood acute lymphoblastic leukemia (ALL) has increased while that of Philadelphia-positive (Ph+) ALL remains low. CD19 is a B-cell specific molecule related to the survival and proliferation of normal B cells. However, there is little information available on the effects of CD19 on the biological behavior of Ph+ ALL cells. Read More

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Identification of integrin heterodimers functioning on the surface of undifferentiated porcine primed embryonic stem cells.

Cell Biol Int 2018 May 29. Epub 2018 May 29.

Department of Animal Life Science, Kangwon National University, Chuncheon, 24341, Korea.

In vitro expansion of undifferentiated porcine primed embryonic stem (ES) cells is facilitated by use of non-cellular niches that mimic three-dimensional (3D) microenvironments enclosing an inner cell mass of porcine blastocysts. Therefore, we investigated the integrin heterodimers on the surface of undifferentiated porcine primed ES cells for the purpose of developing a non-cellular niche to support in vitro maintenance of the self-renewal ability of porcine primed ES cells. Immunocytochemistry and a fluorescence immunoassay were performed to assess integrin α and β subunit levels, and attachment and antibody inhibition assays were used to evaluate the function of integrin heterodimers. Read More

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May 2018
1 Read

Transcriptional noise in intact and TGF-beta treated human kidney cells; the importance of time-series designs.

Cell Biol Int 2018 May 26. Epub 2018 May 26.

Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

The transforming growth factor (TGF)-β signaling pathway plays a key role in various cellular processes. However, insufficient knowledge about the complex and sometimes paradoxical functions of this pathway hinders its therapeutic targeting. In this study, the transcriptional profile of seven mediators and downstream elements of the TGF-β pathway were assessed in TGF-β treated and untreated human kidney derived cells for 2 weeks in a time course manner. Read More

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May 2018
3 Reads

Sphingosine-1-phosphate promotes the proliferation and attenuates apoptosis of Endothelial progenitor cells via S1PR1/S1PR3/PI3K/Akt pathway.

Cell Biol Int 2018 May 23. Epub 2018 May 23.

Department of Cardiology, Wuhan General Hospital of PLA, Wuhan 430070, China.

Sphingosine-1-phosphate (S1P) is a bioactive lysophospholipid that involves in numerous pathophysiological processes. Endothelial progenitor cells (EPCs) play a crucial role in endothelial repair and tumor angiogenesis. The aim of study was to determine the effects of S1P on proliferation and anti-apoptosis of EPCs and their signaling pathways. Read More

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Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats.

Cell Biol Int 2018 May 17. Epub 2018 May 17.

Department of Anatomy, Institute of Biosciences, São Paulo State University (Botucatu campus), Botucatu, São Paulo, Brazil.

Testosterone is often recommended in the treatment of several aging-related conditions. However, there are still questions about the consequences of this therapy in terms of hormonal and inflammatory parameters that are crucial for prostate homeostasis. Thus, we investigate if the testosterone therapy (TT) modulates the hormone receptors and inflammatory cytokines in the ventral prostate of adult rats. Read More

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May 2018
4 Reads

Colchicine, a microtubule-disassembling drug, in the therapy of cardiovascular diseases.

Cell Biol Int 2018 Aug 19;42(8):1079-1084. Epub 2018 Jun 19.

Department of Anatomy and Cell Biology, Medical University, BG-9002 Varna, Bulgaria.

Colchicum autumnale, from which colchicine has been isolated more than 100 years ago, has been used as a treatment for pain and swelling for thousands of years. It is one of the few drugs known from that time period whose use has survived to modernity. Over the past decades, advances in the knowledge of (i) cytoskeletal microtubules (МТ), and (ii) anti-inflammatory and anti-fibrotic effects of colchicine, a classical MT-disassembling (tubulin-targeting) agent, have led to potential new uses for this very old drug extended beyond acute gouty arthritis and familial Mediterranean fever. Read More

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August 2018
2 Reads

TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

Cell Biol Int 2018 May 15. Epub 2018 May 15.

Department of Laboratory Medicine, Yuhuangding Hospital of Qingdao University, Yantai, P. R. China.

Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukemia (CML) and explore the related mechanism. Read More

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H O induces PP2A demethylation to downregulate mTORC1 signaling in HEK293 cells.

Cell Biol Int 2018 May 12. Epub 2018 May 12.

Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Nanning, Guangxi, 530021, China.

Mammalian target of rapamycin (mTOR) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to govern cell growth and proliferation by mediating mitogen- and nutrient-dependent signal transduction. Protein phosphatase 2A (PP2A), a ubiquitously expressed serine/threonine phosphatase, negatively regulates mTOR signaling. Methylation of PP2A is catalyzed by leucine carboxyl methyltransferase-1 (LCMT1) and reversed by protein phosphatase methylesterase 1 (PME-1), which regulates PP2A activity and substrate specificity. Read More

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Effects of VEGF-ANG-1-PLA nano-sustained release microspheres on proliferation and differentiation of ADSCs.

Cell Biol Int 2018 Aug 8;42(8):1060-1068. Epub 2018 Jun 8.

Department of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang, 325000, China.

The improvement of fat graft viability might depend on the presence of multipotent resident adipose derived stem cells (ADSCs) which is the important component of stromal vascular fraction (SVF). Vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) are responsible for neovascularization. However, their half-life is too short to produce a biological effect. Read More

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August 2018
7 Reads

Myricetin-induced oxidative stress suppresses murine T lymphocyte activation.

Cell Biol Int 2018 Aug 10;42(8):1069-1075. Epub 2018 May 10.

Department of Pathology, Dalhousie University, Halifax, NS, Canada.

A number of polyphenolic compounds present in fruits and vegetables have the capacity to modulate immune responses; however, the impact of the common plant-derived flavonoid myricetin on T lymphocyte function has not been investigated. We show that myricetin inhibited mouse T lymphocyte activation by bead-immobilized anti-CD3 and anti-CD28 monoclonal antibodies, as indicated by a dose-dependent reduction in cell proliferation and decreased synthesis of interferon-γ, interleukin (IL)-2, IL-4, and IL-17 associated with different T helper cell subsets. This effect was attributed to myricetin-induced reactive oxygen species (ROS) since myricetin caused hydrogen peroxide (H O ) to accumulate in cell-free culture medium and H O inhibited T cell proliferation and cytokine synthesis. Read More

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MicroRNA-150 restores endothelial cell function and attenuates vascular remodeling by targeting PTX3 through the NF-κB signaling pathway in mice with acute coronary syndrome.

Cell Biol Int 2018 May 9. Epub 2018 May 9.

Department of Cardiovascular, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, 215002, P. R. China.

MicroRNAs (miRNAs) have been known to function as important regulators in the vascular system, with various physiopathological effects such as vascular remodeling and hypertension modulation. We aimed to explore whether microRNA-150 (miR-150) regulates endothelial cell function and vascular remodeling in acute coronary syndrome (ACS), and the involvement of PTX3 and NF-κB signaling pathway. Ten normal mice and sixty ApoE mice were chosen, and their coronary artery tissues and endothelial cells were extracted. Read More

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May 2018
2 Reads

Angiotensin II enhances the acetylation and release of HMGB1 in RAW264.7 macrophage.

Cell Biol Int 2018 May 9. Epub 2018 May 9.

Department of Immunology, Jiangsu University, Zhenjiang, 212013, China.

The high-mobility group box-1 (HMGB1), as a highly conserved ubiquitous DNA-binding protein, has been widely studied in various diseases, including inflammation and tumor; however, fewer studies were focused on the mechanisms controlling HMGB1 release compared with the function of HMGB1. Previous studies have proven that ANG II can act as a pro-inflammatory cytokine, both of HMGB1 and ANG II were significantly upregulated in autoimmune diseases, however, the exact role of ANG II in regulating HMGB1 release have not been shown. The present study was to define the effects of ANG II on macrophages and the possible mechanisms in controlling HMGB1 release. Read More

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Protective effect of retinoic acid receptor α on hypoxia-induced epithelial to mesenchymal transition of renal tubular epithelial cells associated with TGF-β/MMP-9 pathway.

Cell Biol Int 2018 Aug 29;42(8):1050-1059. Epub 2018 May 29.

Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.

Retinoic acid receptor α (RARα), a member of family of the nuclear retinoic acid receptors (RARs), plays an essential role in various chronic kidney diseases (CKD). Renal tubular epithelial to mesenchymal transition (EMT) is a common mechanism of progression of renal interstitial fibrosis (RIF). Hypoxia has been extensively considered as one of major inducers of renal tubular EMT. Read More

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Regulation of inward rectifier potassium current ionic channel remodeling by AT -Calcineurin-NFAT signaling pathway in stretch-induced hypertrophic atrial myocytes.

Cell Biol Int 2018 May 2. Epub 2018 May 2.

Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang 550002, China.

Previous studies have shown that the activation of angiotensin II receptor type I (AT ) is attributed to cardiac remodeling stimulated by increased heart load, and that it is followed by the activation of the calcineurin-nuclear factor of activated T-cells (NFAT) signaling pathway. Additionally, AT has been found to be a regulator of cardiocyte ionic channel remodeling, and calcineurin-NFAT signals participate in the regulation of cardiocyte ionic channel expression. A hypothesis therefore follows that stretch stimulation may regulate cardiocyte ionic channel remodeling by activating the AT -calcineurin-NFAT pathway. Read More

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May 2018
5 Reads

Overexpression of genes associated with hypoxia in cattle adapted to Trans Himalayan region of Ladakh.

Cell Biol Int 2018 May 2. Epub 2018 May 2.

ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India.

Ladakh is an important part of the Trans-Himalayan region located between the Kunlun mountain range in the north and the main Great Himalayas to the south in the state of Jammu and Kashmir of India. The local cattle from Leh and Ladakh region, known as "Ladakhi cattle" is a unique germplasm having an excellent adaptation potential to high altitude hypobaric stress. In the present study, an effort was made to evaluate the transcriptional pattern of hypoxia inducing factor-1 (HIF-1) and several of its regulated genes in PBMCs of local Ladakhi cattle, Holstein Frisian crosses, Jersey (exotic) maintained at high altitude region and Sahiwal (Bos indicus) and Karan Fries (cross bred) cattle maintained in tropical environment. Read More

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May 2018
2 Reads

DL-3-n-butylphthalide promotes dendrite development in cortical neurons subjected to oxygen-glucose deprivation/reperfusion.

Cell Biol Int 2018 Aug 22;42(8):1041-1049. Epub 2018 May 22.

Affiliated Bayi Brain Hospital, The PLA Army General Hospital, Beijing, 100700, China.

The limited degree of functional recovery is closely associated with the condition of the periinfarct cortex after ischemic stroke. The model of oxygen-glucose deprivation in cultured neurons could partly simulate this condition. Proper dendritic morphology is crucial for the correct wiring of neuronal function. Read More

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August 2018
1 Read

Effects of COL8A1 on the proliferation of muscle-derived satellite cells.

Cell Biol Int 2018 Apr 25. Epub 2018 Apr 25.

Laboratory of Cellular and Developmental Biology, Life Science College, North-east Agricultural University, Harbin 150030, China.

Collagen type VIII alpha 1 chain (COL8A1) is a component of the extracellular matrix. Our previous studies suggested that COL8A1 is associated with the proliferation of muscle-derived satellite cells (MDSCs). Additionally, it has been demonstrated that COL8A1 promotes the proliferation of smooth muscle cells and liver cancer cells. Read More

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Molecular interactions between vinculin and phospholipids.

Cell Biol Int 2018 Aug 22;42(8):1076-1078. Epub 2018 May 22.

Department of Physics, Biophysics Group, Friedrich-Alexander-University Erlangen-Nuremberg, D-91052, Erlangen, Germany.

The focal adhesion protein vinculin has been implicated in associating with soluble and membranous phospholipids. Detailed investigations over the past ten years describe the intermolecular interactions of the vinculin tail domain with soluble and membrane phospholipids. Previous studies have implied that the tail's unstructured C-terminal region affects the mechanical behavior of cells and that the same region, at the molecular level, has bi-stable behavior sensitive to different protonation states. Read More

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TREM2 inhibits inflammatory responses in mouse microglia by suppressing the PI3K/NF-κB signaling.

Cell Biol Int 2018 Apr 16. Epub 2018 Apr 16.

Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, P. R. China.

This study aimed to investigate the effects of triggering receptor expressed on myeloid cell-2 (TREM2) on the production of pro-inflammatory mediators and cytokines induced by lipopolysaccharide (LPS) in BV2 microglia. TREM2 expression or TREM2-specific siRNA were used to induce TREM2 overexpression or silencing. The BV2 cells were pre-treated with the PI3 K inhibitor of LY294002 for 1 h and stimulated with LPS for 24 h. Read More

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April 2018
1 Read

Lefty Promotes the Proliferation and Invasion of Trophoblast Cells by Inhibiting Nodal Expression.

Cell Biol Int 2018 Apr 16. Epub 2018 Apr 16.

Obstetric Department, The Maternity Hospital of DaLian, DaLian 116033, China.

Trophoblast cells play a vital role in embryo implantation. Exploring cytokines secreted by trophoblast cells will be significant in revealing the molecular mechanism of embryo implantation. In vitro-cultured trophoblast cells treated with Lefty revealed that Lefty could downregulate the expression of Nodal while promoting the expression of MMP-2 and MMP-9. Read More

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April 2018
1 Read

Role of NCoR1 in mitochondrial function and energy metabolism.

Cell Biol Int 2018 Jun 8;42(6):734-741. Epub 2018 May 8.

Obesity and Comorbidities Research Center, Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil.

Mitochondrial number and shape are constantly changing in response to increased energy demands. The ability to synchronize mitochondrial pathways to respond to energy fluctuations within the cell is a central aspect of mammalian homeostasis. This dynamic process depends on the coordinated activation of transcriptional complexes to promote the expression of genes encoding for mitochondrial proteins. Read More

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June 2018
2 Reads

MiR-340 suppresses the metastasis by targeting EphA3 in cervical cancer.

Cell Biol Int 2018 Apr 16. Epub 2018 Apr 16.

General Medicine, Harrison International Peace Hospital, Hengshui, Hebei province, China.

MicroRNAs (miRNAs) play key roles in cervical cancer metastasis progression. Accumulated evidences have revealed that miRNAs are related to the pathophysiological process. However, the role of miR-340 in cervical cancer and how it works is still not fully interpreted. Read More

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April 2018
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Hypoxia induces apoptosis of mouse spermatocyte GC-2 cells through activation of autophagy.

Cell Biol Int 2018 Apr 16. Epub 2018 Apr 16.

Reproductive Medicine Center, The First Affiliated Hospital of SooChow University, SuZhou 215006, China.

The aim of this study was to investigate the underlying mechanisms of hypoxia-induced apoptosis of GC-2spd (GC-2) cells. The GC-2 cells were treated with or without hypoxia for 12, 24, 48 and 72 h. Apoptosis of GC-2 cells was detected using TUNEL and flow cytometry. Read More

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April 2018
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The novel truncated isoform of human manganese superoxide dismutase has a differential role in promoting metastasis of lung cancer cells.

Cell Biol Int 2018 Aug 8;42(8):1030-1040. Epub 2018 May 8.

Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou, 510632, China.

Growing evidences have demonstrated alternative splicing makes great contribution to tumor metastasis since multiple protein isoforms from a single gene that often display different functions in the cell. Human manganese superoxide dismutase (hMnSOD) was revealed dysregulation in progress of tumor metastasis, while the effect of hMnSOD isoforms on metastasis remained unclear. In this study, we showed a novel truncated hMnSOD isoform hMnSOD183, which lacked 39 amino acids compared with hMnSOD222. Read More

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August 2018
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Eph/ephrin Family Anchored on Exosome Facilitate Communications between Cells.

Cell Biol Int 2018 Apr 6. Epub 2018 Apr 6.

School of Medical Technology, Xuzhou Medical University, Xuzhou Jiangsu 221000, China.

Interactions of Ephrins and Eph receptors at cell membranes play crucial role in boundary formation and axon guidance. Extracellular vesicles (EVs), such as exosomes, are formed by cells communicating with each other in paracrine or endocrine manner. Until now, it is thought that direct cell-cell contact is necessary for ephrin and Eph receptor signal transduction. Read More

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April 2018
2 Reads