6,561,157 results match your criteria Cell Biology Insights [Journal]

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The Neuroprotective Peptide PACAP1-38 Contributes to Horizontal Cell Development in Postnatal Rat Retina.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):770-778

Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs, Hungary.

Purpose: PACAP1-38, a member of the secretin/glucagon superfamily, is expressed in the developing retina with documented neuroprotective effects. However, its function in retinal cell differentiation has yet to be elucidated. Our goals, therefore, were to identify PAC1 expressing cells morphologically, investigate the PACAP1-38 action functionally, and establish PACAP1-38 regulated events developmentally during the first postnatal week in rat retina. Read More

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http://dx.doi.org/10.1167/iovs.18-25719DOI Listing
February 2019

Optimising the biocompatibility of 3D printed photopolymer constructs in vitro and in vivo.

Biomed Mater 2019 Feb 22. Epub 2019 Feb 22.

Department of Surgery, University of Melbourne Faculty of Medicine Dentistry and Health Sciences, Melbourne, Victoria, AUSTRALIA.

3D printing is a rapid and accessible fabrication technology that engenders creative custom design solutions for cell scaffolds, perfusion systems and cell culture systems for tissue engineering. Critical to its success is the biocompatibility of the materials used, which should allow long-term tissue culture without affecting cell viability or inducing an inflammatory response for in vitro and in vivo applications. Polyjet 3D printers offer arguably the highest resolution with the fewest design constraints of any commercially available 3D printing systems. Read More

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http://dx.doi.org/10.1088/1748-605X/ab09c4DOI Listing
February 2019

Advanced nanostructures for cell membrane poration.

Nanotechnology 2019 Feb 22. Epub 2019 Feb 22.

Mechanical Engineering, University of Colorado Boulder, ECES 158, Boulder, Colorado, 80309-0401, UNITED STATES.

Nanostructured devices have been able to foster the technology for cell membrane poration. With the size smaller than the cell, nanostructure allows efficient poration on the cell membrane. Emerging nanostructures with various physical transduction have been demonstrated to accommodate effective intracellular delivery. Read More

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http://dx.doi.org/10.1088/1361-6528/ab096bDOI Listing
February 2019

Epidermal growth factor-nanoparticle conjugates change the activity from anti-apoptotic to pro-apoptotic at membrane rafts.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

WPI Research Initiative for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan; Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. Electronic address:

The proliferation epidermal growth factor (EGF) is known to acquire contradictory apoptotic activities upon conjugation with gold nanoparticles (GNPs) through hitherto unknown mechanisms. Here, we identified an essential role of membrane rafts in the drastic activity switching of EGF-GNPs through the following intracellular signaling. 1) In contrast to the rapid diffusion of activated EGF receptor after the soluble EGF stimulation, the receptor is confined within membrane rafts upon binding to the EGF-GNPs. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.026DOI Listing
February 2019

A transferrin receptor-binding mucoadhesive elastin-like recombinamer: in vitro and in vivo characterization.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

BIOFORGE (Group for Advanced Materials and Nanobiotechnology), CIBER-BBN, University of Valladolid, Paseo de Belén 19, 47011, Spain. Electronic address:

The development of mucoadhesive materials is of great interest and is also a major challenge. Being adsorption sites, mucosae are suitable targets for drug delivery, but as defensive barriers they are complex biological surfaces to interact with, mainly due to their protective mucus layer. As such, first- and second-generation mucoadhesives focused on material-mucus interactions, whereas the third generation of mucoadhesives introduced structural motifs that are able to interact with the cells beneath the mucus layer. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.027DOI Listing
February 2019

Toward Tissue-engineering of Nasal Cartilages.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

Department of Biomedical Engineering, University of California Irvine, 3120 Natural Sciences II, Irvine, CA, 92697-2715, USA. Electronic address:

Nasal cartilage pathologies are common; for example, up to 80% of people are afflicted by deviated nasal septum conditions. Because cartilage provides the supportive framework of the nose, afflicted patients suffer low quality of life. To correct pathologies, graft cartilage is often required. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.025DOI Listing
February 2019

Effect of magnesium-degradation products and hypoxia on the angiogenesis of human umbilical vein endothelial cells.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

Institute of Materials Research, Division for Metallic Biomaterials, Helmholtz-Zentrum Geesthacht (HZG), Geesthacht, Germany. Electronic address:

Biodegradable magnesium (Mg) metals have been applied in orthopaedic and stent applications due to their biodegradability, bioabsorbability and adaptability to tissue regeneration. However, further investigations are still needed to understand how angiogenesis will respond to high concentrations of Mg and oxygen content differences, which are vital to vascular remodelling and bone fracture regeneration or tissue healing. Human primary endothelial cells were exposed to various concentrations (2 to 8 mM) of extracellular Mg degradation products under either hypoxia or normoxia. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.018DOI Listing
February 2019

Analytical Validation of Variants to Aid in Genotype-Guided Therapy for Oncology.

J Mol Diagn 2019 Feb 19. Epub 2019 Feb 19.

Department of Medicine, Division of Clinical Pharmacology.

The Clinical Laboratory Improvement Amendments (CLIA) of 1988 requires that pharmacogenetic genotyping methods need to be established according to technical standards and laboratory practice guidelines before testing can be offered to patients. Testing methods for variants in ABCB1, CBR3, COMT, CYP3A7, C8ORF34, FCGR2A, FCGR3A, HAS3, NT5C2, NUDT15, SBF2, SEMA3C, SLC16A5, SLC28A3, SOD2, TLR4, and TPMT were validated in a CLIA-accredited laboratory. As no known reference materials were available, DNA samples that were from Coriell Cell Repositories (Camden, NJ) were used for the analytical validation studies. Read More

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http://dx.doi.org/10.1016/j.jmoldx.2019.01.009DOI Listing
February 2019

Diagnostic and prognostic potential of serum cell-free microRNA-214 in glioma.

World Neurosurg 2019 Feb 19. Epub 2019 Feb 19.

Department of Neurosurgery, Linyi People's Hospital, Shandong University, Linyi, Shandong Province, China. Electronic address:

Objectives: We aimed to expound feasibility of serum cell-free microRNA-214 (miR-214) as a noninvasive biomarker for glioma in this study.

Patients And Methods: We detected expression of miR-214 in medium from 2 glioma cell lines to confirm whether it is secretory in screening phase. Then we verified cell-free miR-214 expression in serum samples from an independent set of 100 preoperative glioma patients, 30 matching postoperative patients and 100 healthy controls. Read More

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http://dx.doi.org/10.1016/j.wneu.2019.02.009DOI Listing
February 2019

Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing.

Nanomedicine 2019 Feb 19. Epub 2019 Feb 19.

Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, USA; Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, USA; Department of Surgery, Penn State Milton S. Hershey Medical Center, Hershey, PA, USA. Electronic address:

A nanotube assisted microwave electroporation (NAME) technique is demonstrated for delivering molecular biosensors into viable bacteria for multiplex single cell pathogen identification to advance rapid diagnostics in clinical microbiology. Due to the small volume of a bacterial cell (~femtoliter), the intracellular concentration of the target molecule is high, which results in a strong signal for single cell detection without amplification. The NAME procedure can be completed in as little as 30minutes and can achieve over 90% transformation efficiency. Read More

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http://dx.doi.org/10.1016/j.nano.2019.01.015DOI Listing
February 2019

Nanoparticle physicochemical properties determine the activation of intracellular complement.

Nanomedicine 2019 Feb 19. Epub 2019 Feb 19.

Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD. Electronic address:

The complement system plays an essential role in both innate and adaptive immunity. The traditional understanding of this system comes from studies investigating complement proteins produced by the liver and present in plasma to "complement" the immune cell-mediated response to invading pathogens. Recently, it has been reported that immune cells including, but not limited to, T-cells and monocytes, express complement proteins. Read More

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http://dx.doi.org/10.1016/j.nano.2019.02.002DOI Listing
February 2019

Gene cloning and expression of a partial sequence of Hirudomacin, an antimicrobial protein that is increased in leech (Hirudo nipponica Whitman) after a blood meal.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 19. Epub 2019 Feb 19.

Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.

The novel antimicrobial gene Hirudomacin (Hmc), with a 249-bp cDNA, encodes a mature protein of 61 amino acids and a 22-amino acid signal peptide. Hmc exhibits the highest similarity, at 90.1%, with macin family members found in the salivary gland of the leech Hirudo nipponica Whitman. Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.02.005DOI Listing
February 2019

Sex biased expression of anti-Mullerian hormone (amh) gene in a live bearing fish, Gambusia holbrooki: Evolutionary implications and potential role in sex differentiation.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 19. Epub 2019 Feb 19.

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania, TAS, Australia; Inland Fisheries and Services (IFS), TAS, Australia.

The amh, a member of transforming growth factor-β (TGF-β) family, is known to play a critical role in vertebrate male sex differentiation, with its paralogue/s evolving to determine sex in few heterogametic (XX/XY) teleosts. However, it remains relatively unexplored in the reproductively unique live bearing teleosts. Therefore, this study comparatively examined the structure and content of G. Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.02.004DOI Listing
February 2019

Heterologous expression of an alternative oxidase from Moniliophthora perniciosa in Saccharomyces cerevisiae: antioxidant function and in vivo platform for the study of new drugs against witches' broom disease.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Laboratório de Biologia Molecular e Biotecnologia Industrial de Microrganismos, Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. Electronic address:

The fungus Moniliophthora perniciosa is the causal agent of witches' broom disease (WBD), one of the most devastating diseases of cacao, the chocolate tree. Many strategies to control WBD have been tested so far, including the use of agrochemicals such as the strobilurins. Strobilurins are fungicides of the QoI family, and they are used in the control of a wide array of fungal diseases in many different crops, including cereals, field crops, fruits, tree nuts, and vegetables. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.006DOI Listing
February 2019

Genotypic and Phenotypic Characterization of Candida albicans Lebanese Hospital Isolates Resistant and Sensitive to Caspofungin.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Natural Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon(1). Electronic address:

The fungus Candida albicans is both a commensal and an opportunistic human pathogen, present as part of the normal human microflora causing serious mucosal, and systemic life threatening infections. The antifungal drug caspofungin of the echinocandin family is the latest generation of antifungal drugs to be developed. It functions by inhibiting glucan synthase thus weakening the fungal cell wall leading to death. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.008DOI Listing
February 2019

A strikingly-angled spindle mediates nuclear migration during colonization of rice cells infected by Magnaporthe oryzae.

Fungal Genet Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Plant Biology, University of Georgia, Athens, GA 30602, USA. Electronic address:

To cause rice blast disease, Magnaporthe oryzae must properly organize microtubules and position nuclei during colonization of host cells. Live cell confocal imaging of fluorescently-tagged microtubules and nuclei of M. oryzae invasive hyphae reveals that microtubules form a cage-like arrangement around nuclei during interphase and that the mitotic spindle forms and mediates nuclear migration while integrity of the nuclear envelope is lost. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.005DOI Listing
February 2019

The optimum temperature for the heat therapy for meibomian gland dysfunction.

Authors:
Douglas Borchman

Ocul Surf 2019 Feb 19. Epub 2019 Feb 19.

Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY, 40202, USA. Electronic address:

Purpose: Numerous devices have been developed to warm the eyelid as a therapy for Meibomian gland dysfunction. The optimum temperature for such therapy was determined.

Methods: Meibum lipid disorder versus temperature was calculated from previously published phase transition parameters measured using infrared spectroscopy. Read More

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http://dx.doi.org/10.1016/j.jtos.2019.02.005DOI Listing
February 2019

Increased CD16 expression on NK cells is indicative of antibody-dependent cell-mediated cytotoxicity in chronic-active antibody-mediated rejection.

Transpl Immunol 2019 Feb 19. Epub 2019 Feb 19.

Department of Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands.

Chronic-active antibody mediated rejection (c-aABMR) contributes significantly to late renal allograft failure. The antibodies directed against donor-derived antigens, e.g. Read More

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http://dx.doi.org/10.1016/j.trim.2019.02.005DOI Listing
February 2019

Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut.

Fish Shellfish Immunol 2019 Feb 19. Epub 2019 Feb 19.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; CAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266400, China. Electronic address:

In order to understand the mediation function of surface proteins in probiotic effects executed by Lactobacillus pentosus HC-2 in midgut of Litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal HC-2 or with stripped surface proteins HC-2 by lithium chloride (LiCl) treatment. The results showed that the shrimp fed with normal HC-2 produced much higher immune and digestion related enzymes than the control group or LiCl-treated HC-2 group to defense the Vibrio parahaemolyticus E1 infection. We obtained total over 275,099 unigenes from L. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.027DOI Listing
February 2019

Autologous Transplantation for Male Germ Cell Tumors: Improved Outcomes over 3 decades.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Medical College of Wisconsin, Milwaukee, WI.

Purpose: The curative potential of autologous hematopoietic cell transplantation (autoHCT) for male germ cell tumors (GCT) is well established. Optimal timing and number (single, ST vs. tandem, TT vs. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.015DOI Listing
February 2019

AUTOLOGOUS HEMATOPOIETIC CELL TRANSPLANTATION FOR TREATMENT-REFRACTORY RELAPSING MULTIPLE SCLEROSIS: POSITION STATEMENT FROM THE AMERICAN SOCIETY FOR BLOOD AND MARROW TRANSPLANTATION.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Multiple sclerosis (MS) is a chronic, disabling, immune-mediated, central nervous system demyelinating and degenerative disease. Approved disease modifying therapies may be incompletely effective in some patients with highly active relapsing disease and high risk of disability. Immunoablative or myeloablative therapy followed by autologous hematopoietic cell transplantation (AHCT) has been investigated in retrospective studies, clinical trials, and meta-analyses/systematic reviews as an approach to address this unmet clinical need. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.014DOI Listing
February 2019

Risk factors and predictive scoring system for post-transplant lymphoproliferative disorder after hematopoietic stem cell transplantation.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Department of Oncology and Hematology, Shimane University Hospital, Izumo, Japan. Electronic address:

We analyzed data of 64,539 consecutive patients in the national Japanese transplant registry, including 40,195 after allogeneic stem cell transplantation, 24,215 after autologous stem cell transplantation, and 129 after syngeneic stem cell transplantation, and 299 developed Epstein-Barr virus positive post-transplant lymphoproliferative disorder. The probability at 2 years was 0.79% after allogeneic transplantation, 0. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.016DOI Listing
February 2019

Targeting ERK1/2 protein-serine/threonine kinases in human cancers.

Authors:
Robert Roskoski

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC, 28742-8814, United States. Electronic address:

ERK1 and ERK2 are key protein kinases that contribute to the Ras-Raf-MEK-ERK MAP kinase signalling module. This pathway participates in the control of numerous processes including apoptosis, cell proliferation, the immune response, nervous system function, and RNA synthesis and processing. MEK1/2 activate human ERK1/2 by first catalyzing the phosphorylation of Y204/187 and then T202/185, both residues of which occur within the activation segment. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.01.039DOI Listing
February 2019

Dihydrotanshinone exhibits an anti-inflammatory effect in vitro and in vivo through blocking TLR4 dimerization.

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. Electronic address:

Dihydrotanshinone (DHT), one of the major ingredients of Salvia miltiorrhiza Bunge (Danshen), displays many bioactivities. However, the activity and underlying mechanism of DHT in anti-inflammation have not yet been elucidated. In this study, we investigated the anti-inflammatory activity and molecular mechanism of action of DHT both in vitro and in vivo. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.017DOI Listing
February 2019

The acyl-glucuronide metabolite of ibuprofen has analgesic and anti-inflammatory effects via the TRPA1 channel.

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Florence, Italy. Electronic address:

Ibuprofen is a widely used non-steroidal anti-inflammatory drug (NSAID) that exerts analgesic and anti-inflammatory actions. The transient receptor potential ankyrin 1 (TRPA1) channel, expressed primarily in nociceptors, mediates the action of proalgesic and inflammatory agents. Ibuprofen metabolism yields the reactive compound, ibuprofen-acyl glucuronide, which, like other TRPA1 ligands, covalently interacts with macromolecules. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.019DOI Listing
February 2019

Aromatic monoterpenoid glycosides from rattan stems of Schisandra chinensis and their neuroprotective activities.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin 150040, China. Electronic address:

Six new monoterpenoid glycosides, including thymoquinol 5-O-α-L-arabinopyranosyl-(1 → 6)-β-D-glucopyranoside (1), cuminic acid 7-O-α-D-arabinofuranosyl-(1 → 6)-β-d-glucopyranosyl ester (2), p-cymene 7-O-α-D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (3), p-methylhyd- ratropic acid 9-O-α-D-arabinofuranosyl-(1 → 6)-β-d-glucopyranosyl ester (4), (R)-p-cymene 9-O-α- D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (5), and (R)-p-cymene 9-O-β-D-apiofuranosyl-(1 → 6)-β-D-glucopyranoside (6), together with three known compounds, such as (R)-p-cymene 9-O-β-D-glucopyranoside (7), thymoquinol 5-O-β-D-glucopyranoside (8), and thymoquinol 2-O-β-D-glucopyranoside (9) were isolated from the 70%-EtOH extract of Schisandra chinensis rattan stems. The structures of the new compounds were elucidated by detailed spectroscopic analyses. Meanwhile, the neuroprotective activities of compounds 1-9 were evaluated on the two co-culture models of microglia and neurons cells and astrocytoma and neurons cells. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.012DOI Listing
February 2019

Diverse lignans with anti-inflammatory activity from Urceola rosea.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Seventeen structurally diverse lignans, comprising six new compounds, ecdysanols A (1), B (11), C - E (13-15), and F (17), were isolated from the caulis of Urceola rosea. The structures and absolute configurations of these new compounds were elucidated by means of extensive analysis of mass and NMR data, as well as chiroptical properties. A bioassay in vitro showed that all lignans possessed anti-inflammatory effects by inhibiting the production of TNF-α, NO and/or IL-6 in LPS-stimulated RAW264. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.011DOI Listing
February 2019
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Bis-iridoid and iridoid glycosides: Viral protein R inhibitors from Picrorhiza kurroa collected in Myanmar.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama 930-0194, Japan. Electronic address:

Four new bis-iridoid glycosides, saungmaygaosides A-D (1-4), and six known iridoid glycosides (5-10) were isolated from the n-butanol extract of the stems of Picrorhiza kurroa collected in Myanmar. Their structures were elucidated by extensive spectroscopic techniques. All of the isolates were assayed for anti-Vpr activity, using TREx-HeLa-Vpr cells. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.016DOI Listing
February 2019

Common pathogenic mechanism in patients with dropped head syndrome caused by different mutations in the MYH7 gene.

Gene 2019 Feb 19. Epub 2019 Feb 19.

Medical Genetics Laboratory, Petrovsky Russian Research Center of Surgery, Moscow 119991, Russia; Pirogov Russian National Research Medical University, Moscow 117997, Russia.

Mutations in the MYH7 gene are the source of an allelic series of diseases, including various cardiomyopathies and skeletal myopathies that usually manifest in adulthood. We observed a 1.5 y. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.011DOI Listing
February 2019

Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma.

Gene 2019 Feb 19. Epub 2019 Feb 19.

Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China. Electronic address:

The pathogenesis and the underlying mechanisms of hepatocellular carcinoma (HCC) remain unclear. LncRNA plasmacytoma variant translocation 1 (PVT1) is an oncogene in a variety of cancers. The role of PVT1 in HCC progression is not completely understood. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.036DOI Listing
February 2019

Free energy calculations of RNA interactions.

Methods 2019 Feb 19. Epub 2019 Feb 19.

Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE-751 24, Uppsala, Sweden. Electronic address:

This review discusses the use of molecular dynamics free energy calculations for characterizing RNA interactions, with particular emphasis on molecular recognition events involved in mRNA translation on the ribosome. The general methodology for efficient free energy calculations is outlined and our specific implementation for binding free energy changes due to base mutations in mRNA and tRNA is described. We show that there are a number of key problems related to the accuracy of protein synthesis that can be addressed with this type of computational approach and several such examples are discussed in detail. Read More

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http://dx.doi.org/10.1016/j.ymeth.2019.02.014DOI Listing
February 2019

Self-assembly, functionality, and in-vitro properties of quercetin loaded nanoparticles based on shellac-almond gum biological macromolecules.

Int J Biol Macromol 2019 Feb 19. Epub 2019 Feb 19.

Particle and Interfacial Technology Group (PaInT), Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Gent 9000, Belgium.

Quercetin-fortified nanoparticles were prepared from almond gum (AG), a novel biological macromolecule, and Tween 80 (T80) as stabilizers and shellac (SH) as core material using an antisolvent precipitation method. The final nanoparticles were prepared by 0.67% SH, 0. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.02.071DOI Listing
February 2019

Drug repurposing for new, efficient, broad spectrum antivirals.

Virus Res 2019 Feb 19. Epub 2019 Feb 19.

Cell Structure Laboratory, National Center for Biotechnology, National Research Council, CNB-CSIC, Darwin 3, UAM, campus de Cantoblanco, 28049 Madrid, Spain. Electronic address:

Emerging viruses are a major threat to human health. Recent outbreaks have emphasized the urgent need for new antiviral treatments. For several pathogenic viruses, considerable efforts have focused on vaccine development. Read More

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http://dx.doi.org/10.1016/j.virusres.2019.02.011DOI Listing
February 2019

Co-expression of cytokeratin and vimentin in colorectal Cancer highlights a subset of tumor buds and an atypical Cancer-associated stroma.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Institute of Pathology, University of Bern, Murtenstrasse 31, Bern, CH-3008. Electronic address:

Tumor buds in colorectal cancer are hypothesized to undergo a (partial) epithelial-mesenchymal transition (EMT). If so, Cytokeratin (CK) and Vimentin (VIM) co-expression is expected. CK+/VIM+ can also be found in some stromal cells, however their origin remains unclear. Read More

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http://dx.doi.org/10.1016/j.humpath.2019.02.002DOI Listing
February 2019

FAM134B promotes esophageal squamous cell carcinoma in vitro and its correlations with clinicopathologic features.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Australia. Electronic address:

Family with sequence similarity 134, member B (FAM134B) is an autophagy regulator of endoplasmic reticulum first discovered to be involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC). The present study examined the functional behavior of FAM134B in cancer cells and the association of FAM134B expression with clinicopathologic factors in patients with ESCC. Expression at both the mRNA and protein levels was investigated using real-time polymerase chain reaction and immunohistochemistry. Read More

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http://dx.doi.org/10.1016/j.humpath.2018.11.033DOI Listing
February 2019

Tumor PD-L1 expression in malignant pleural and peritoneal mesothelioma by Dako PD-L1 22C3 pharmDx and Dako PD-L1 28-8 pharmDx assays.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pathology, University of Utah, 1950 Circle of Hope, Salt Lake City, UT 84112; ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT, 84108.

Malignant mesothelioma (MM) is an aggressive neoplasm with poor prognosis. The Dako PD-L1 22C3 and 28-8 pharmDx assays are approved by the US Food and Drug Administration (FDA) as companion and complementary diagnostics for the anti-PD-1 drugs pembrolizumab and nivolumab, respectively. Data from multiple clinical trials indicate that immunotherapy has anti-tumor activity in advanced malignant pleural (MPM) and peritoneal mesothelioma (MPeM). Read More

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http://dx.doi.org/10.1016/j.humpath.2019.02.001DOI Listing
February 2019

Reactive cholangiocytes differentiate into proliferative hepatocytes with efficient DNA repair in mice with chronic liver injury.

J Hepatol 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Hepato-gastroenterology, Institut de Recherche Expérimentale et Clinique, Université catholique de Louvain, Brussels, Belgium. Electronic address:

Background And Aim: Chronic liver diseases are characterized by expansion of the small immature cholangiocytes - a mechanism named ductular reaction (DR) - which have the capacity to differentiate in hepatocytes. We investigated the kinetics of DR differentiation into hepatocytes as well as several important features as functional maturity, clonal expansion and resistant to stress of the newly formed hepatocytes in mice with long-term liver damage.

Methods: We tracked cholangiocytes using osteopontin-iCreER and hepatocytes with AAV8-TBG-Cre. Read More

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http://dx.doi.org/10.1016/j.jhep.2019.02.003DOI Listing
February 2019

Multicellular systems biology: quantifying cellular patterning and function in plant organs using network science.

Authors:
George W Bassel

Mol Plant 2019 Feb 19. Epub 2019 Feb 19.

School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK. Electronic address:

Organ function is at least partially shaped and constrained by the organization of their constituent cells. Extensive investigation has revealed mechanisms explaining how these patterns are generated, with less being known about their functional relevance. In this paper, a methodology to discretize and quantitatively analyze cellular patterning is described. Read More

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http://dx.doi.org/10.1016/j.molp.2019.02.004DOI Listing
February 2019

The use of Platelet-rich Fibrin in the management of medication-related osteonecrosis of the jaw: a case series.

J Stomatol Oral Maxillofac Surg 2019 Feb 19. Epub 2019 Feb 19.

Department of Molecular and Cell Biology, Institute of Biology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.

Medication related osteonecrosis of the jaw (MRONJ) is characterized by exposed necrotic bone in the maxillofacial region that persists for more than eight weeks in patients taking antiresorptive or antiangiogenic drugs for bone metastasis or osteoporosis. The management of such condition depends on several factors, among which the staging of MRONJ. Though, a specific gold standard treatment has not been established to date. Read More

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http://dx.doi.org/10.1016/j.jormas.2019.02.011DOI Listing
February 2019

Biomaterializing the promise of cardiac tissue engineering.

Biotechnol Adv 2019 Feb 19. Epub 2019 Feb 19.

Division of Cardiology, Department of Medicine, Duke University Health System, Durham, NC, USA; Department of Biomedical Engineering, Duke University, Durham, NC, USA. Electronic address:

During an average individual's lifespan, the human heart pumps nearly 200 million liters of blood delivered by approximately 3 billion heartbeats. Therefore, it is not surprising that native myocardium under this incredible demand is extraordinarily complex, both structurally and functionally. As a result, successful engineering of adult-mimetic functional cardiac tissues is likely to require utilization of highly specialized biomaterials representative of the native extracellular microenvironment. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.02.009DOI Listing
February 2019

Exopolysaccharides synthesized by Bifidobacterium animalis subsp. lactis interact with TLR4 in intestinal epithelial cells.

Anaerobe 2019 Feb 19. Epub 2019 Feb 19.

Microhealth Group, Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias - Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Asturias, Spain. Electronic address:

The toll-like receptors involved in recognition of the exopolysaccharide produced by two isogenic, ropy and non-ropy, Bifidobacterium animalis subsp. lactis strains were investigated. Both strains interact with human embryonic kidney (HEK)-293 cells via TLR2, whereas purified EPSs specifically stimulate TLR4 regardless their molar mass. Read More

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http://dx.doi.org/10.1016/j.anaerobe.2019.02.014DOI Listing
February 2019

Endothelial dysfunction in pregnancy metabolic disorders.

Biochim Biophys Acta Mol Basis Dis 2019 Feb 19. Epub 2019 Feb 19.

Departamento de Ciencias Biologicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370146 Santiago, Chile; Millennium Institute on Immunology and Immunotherapy, 8331150 Santiago, Chile. Electronic address:

In recent years, the vascular endothelium has gained attention as a key player in the initiation and development of pregnancy disorders. Endothelium acts as an endocrine organ that preserves the homeostatic balance by responding to changes in metabolic status. However, in metabolic disorders, endothelial cells adopt a dysfunctional function, losing their normal responsiveness. Read More

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http://dx.doi.org/10.1016/j.bbadis.2019.02.009DOI Listing
February 2019

Influence of traditionally used Nepalese plants on wound healing and immunological properties using primary human cells in vitro.

J Ethnopharmacol 2019 Feb 19. Epub 2019 Feb 19.

Centre for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg, Breisacher Straße 115 B, 79106 Freiburg, Germany. Electronic address:

Ethnopharmacological Relevance: The improvement of wound healing has always been an important issue for both ethnopharmacological and modern medical research. In this study, we used state-of-the-art methods to investigate extracts of plants used traditionally in Nepal for more than 1,000 years to treat inflammatory skin injuries.

Aim Of The Study: We focused on the potential of the plant extracts to ameliorate wound healing and to influence immune modulatory properties. Read More

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http://dx.doi.org/10.1016/j.jep.2019.02.034DOI Listing
February 2019

Dihydrotestosterone activates AP-1 in LNCaP prostate cancer cells.

Int J Biochem Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Medical Biochemistry and Molecular Biology, D-66421 Homburg, Germany.

A cross-talk between androgen/androgen receptor signaling and the AP-1 transcription factor has been proposed. In this study, we asked whether activation of AP-1 modifies androgen-responsive gene transcription, and whether androgens effect AP-1-regulated gene transcription. We show that activation of AP-1 via expression of a constitutively active mutant of mitogen-activated/extracellular signal responsive kinase kinase (MEK) kinase-1 did not increase the activity of the androgen-responsive probasin promoter. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.02.005DOI Listing
February 2019

Anti-cancer effect of Indanone-based thiazolyl hydrazone derivative on colon cancer cell lines.

Int J Biochem Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, 11439, USA. Electronic address:

Colorectal cancer is the third leading cause of cancer related deaths in the United States. Currently, Irinotecan, a topoisomerase I inhibitor, is an approved anti-cancer drug for the treatment of patients with advanced or recurrent colorectal cancer. Considering low response rate and events of high toxicity caused by irinotecan, we evaluated a series of thirteen thiazolyl hydrazone derivatives of 1-indanone for their potential antineoplastic activity and four compounds showed promising anti-cancer activity against most of the tested colon cancer cell lines with IC values ranging from 0. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.02.004DOI Listing
February 2019

Protein and surface expression of HCN2 and HCN4 subunits in mesocorticolimbic areas after cocaine sensitization.

Neurochem Int 2019 Feb 19. Epub 2019 Feb 19.

Physiology Department, University of Puerto Rico, School of Medicine, Medical Sciences Campus, San Juan, 00936-5067, Puerto Rico. Electronic address:

The I is a mixed depolarizing current present in neurons which, upon activation by hyperpolarization, modulates neuronal excitability in the mesocorticolimbic (MCL) system, an area which regulates emotions such as pleasure, reward, and motivation. Its biophysical properties are determined by HCN protein expression profiles, specifically HCN subunits 1-4. Previously, we reported that cocaine-induced behavioral sensitization increases HCN2 protein expression in all MCL areas with the Ventral Tegmental Area (VTA) showing the most significant increase. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.02.004DOI Listing
February 2019

Propagermanium, a CCR2 inhibitor, attenuates cerebral ischemia/reperfusion injury through inhibiting inflammatory response induced by microglia.

Neurochem Int 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Natural Medicines, School of Basic Medical Sciences and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China. Electronic address:

CCR2 could recruit immune cells migrating into brain after ischemic stroke. It is unclear whether and why Propagermanium (PG, a CCR2 inhibitor) is able to protect against ischemic injury. Middle cerebral artery occlusion (MCAO) and reperfusion injury in C57BL/6 J male mice were performed in vivo to mimic ischemic stroke. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.02.010DOI Listing
February 2019

Slow dynamics in microcolumnar gap junction network of developing neocortical pyramidal neurons.

Neuroscience 2019 Feb 19. Epub 2019 Feb 19.

RIKEN Center for Brain Science, Wako, Saitama 351-0198, Japan. Electronic address:

Gap junctions mediate electrical coupling between neurons and modulate their firing activity. In mouse neocortical layer 5, the major types of pyramidal neurons organize into cell type-specific microcolumns that exhibit modular neuronal activity. During cortical development, microcolumn neurons are electrically coupled in a cell type-specific manner at the stage of synaptogenesis, forming a dense network of gap junctions. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.02.013DOI Listing
February 2019

Cell pelotons: a model of early evolutionary cell sorting, with application to slime mold D. discoideum.

Authors:
Hugh Trenchard

J Theor Biol 2019 Feb 19. Epub 2019 Feb 19.

Independent researcher, 805 647 Michigan Street, Victoria BC Canada V8V 1S9, 250-472-0718. Electronic address:

A theoretical model is presented for early evolutionary cell sorting within cellular aggregates. The model involves an energy-saving mechanism and principles of collective self-organization analogous to those observed in bicycle pelotons (groups of cyclists). The theoretical framework is applied to slime-mold slugs (Dictyostelium discoideum) and incorporated into a computer simulation which demonstrates principally the sorting of cells between the anterior and posterior slug regions. Read More

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http://dx.doi.org/10.1016/j.jtbi.2019.02.011DOI Listing
February 2019

GABAR isoform and subunit structural motifs determine synaptic and extrasynaptic receptor localisation.

Neuropharmacology 2019 Feb 19. Epub 2019 Feb 19.

Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK. Electronic address:

GABA receptors (GABARs) are the principal inhibitory neurotransmitter receptors in the central nervous system. They control neuronal excitability by synaptic and tonic forms of inhibition mostly mediated by different receptor subtypes located in specific cell membrane subdomains. A consensus suggests that α1-3βγ comprise synaptic GABARs, whilst extrasynaptic α4βδ, α5βγ and αβ isoforms largely underlie tonic inhibition. Read More

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http://dx.doi.org/10.1016/j.neuropharm.2019.02.022DOI Listing
February 2019
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