1,341 results match your criteria Cell Biology And Toxicology[Journal]


Gestational bisphenol A exposure induces fatty liver development in male offspring mice through the inhibition of HNF1b and upregulation of PPARγ.

Cell Biol Toxicol 2020 Jul 4. Epub 2020 Jul 4.

Department of Toxicology, Shaanxi Key Lab of Free Radical Biology and Medicine, the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Air Force Medical University (Fourth Military Medical University), Changle West Road 169, Xi'an, 710032, Shaanxi Province, China.

Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) associated with non-alcoholic fatty liver disease (NAFLD). The effects of gestational BPA exposure on hepatic lipid accumulation in offspring are not fully understood. Here, we investigate the sex-dependent effects of gestational BPA exposure on hepatic lipid and glucose metabolism in the offspring of mice to reveal the mechanisms underlying gestational BPA exposure-associated NAFLD. Read More

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http://dx.doi.org/10.1007/s10565-020-09535-3DOI Listing

Human hepatic in vitro models reveal distinct anti-NASH potencies of PPAR agonists.

Cell Biol Toxicol 2020 Jul 1. Epub 2020 Jul 1.

Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium.

Non-alcoholic steatohepatitis (NASH) is a highly prevalent, chronic liver disease characterized by hepatic lipid accumulation, inflammation, and concomitant fibrosis. Up to date, no anti-NASH drugs have been approved. In this study, we reproduced key NASH characteristics in vitro by exposing primary human hepatocytes (PHH), human skin stem cell-derived hepatic cells (hSKP-HPC), HepaRG and HepG2 cell lines, as well as LX-2 cells to multiple factors that play a role in the onset of NASH. Read More

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http://dx.doi.org/10.1007/s10565-020-09544-2DOI Listing

Single-cell sequencing reveals novel mechanisms of Aflatoxin B1-induced hepatotoxicity in S phase-arrested L02 cells.

Cell Biol Toxicol 2020 Jul 1. Epub 2020 Jul 1.

Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China.

Aflatoxin B1 (AFB1) is widely distributed in nature and is confirmed to be the most toxic of all the aflatoxins, whose predominant metabolism site is the liver. As a well-studied and vital mode of epigenetic modifications, aberrant methylation of the promoters in eukaryotic cells may cause the silence of essential genes, affecting their related transcriptional pathways and ultimately leading to the development of disease and cancers. This study investigated the mechanisms of AFB1-induced hepatotoxicity in S phase-arrested L02 cells using single-cell RNA-seq and single-cell reduced representation bisulfite sequencing (RRBS). Read More

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http://dx.doi.org/10.1007/s10565-020-09547-zDOI Listing

Ion transport mechanisms for smoke inhalation-injured airway epithelial barrier.

Cell Biol Toxicol 2020 Jun 25. Epub 2020 Jun 25.

Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, 11937 US Hwy 271, Tyler, TX, 75708, USA.

Smoke inhalation injury is the leading cause of death in firefighters and victims. Inhaled hot air and toxic smoke are the predominant hazards to the respiratory epithelium. We aimed to analyze the effects of thermal stress and smoke aldehyde on the permeability of the airway epithelial barrier. Read More

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http://dx.doi.org/10.1007/s10565-020-09545-1DOI Listing

An automated and high-throughput-screening compatible pluripotent stem cell-based test platform for developmental and reproductive toxicity assessment of small molecule compounds.

Cell Biol Toxicol 2020 Jun 20. Epub 2020 Jun 20.

Fraunhofer IME ScreeningPort, Schnackenburgallee 114, 22525, Hamburg, Germany.

The embryonic stem cell test (EST) represents the only validated and accepted in vitro system for the detection and classification of compounds according to their developmental and reproductive teratogenic potency. The widespread implementation of the EST, however, in particular for routine application in pharmaceutical development, has not been achieved so far. Several drawbacks still limit the high-throughput screening of potential drug candidates in this format: The long assay period, the use of non-homogeneous viability assays, the low throughput analysis of marker protein expression and the compatibility of the assay procedures to automation. Read More

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http://dx.doi.org/10.1007/s10565-020-09538-0DOI Listing

Acetyl-11-keto-β-boswellic acid enhances the cisplatin sensitivity of non-small cell lung cancer cells through cell cycle arrest, apoptosis induction, and autophagy suppression via p21-dependent signaling pathway.

Cell Biol Toxicol 2020 Jun 20. Epub 2020 Jun 20.

Center for Tumor Diagnosis and Therapy, Jinshan Hospital, Fudan University, Jinshan District, Shanghai, 201508, China.

Cisplatin-based therapy is a widely used chemotherapeutic regimen for non-small cell lung cancer (NSCLC); however, drug resistance limits its efficacy. Acetyl-11-keto-β-boswellic acid (AKBA), a bioactive compound from frankincense, has been shown to exert anti-cancer effects. The aim of this study is to explore the potential of AKBA in combination with cisplatin as a new regimen for NSCLC. Read More

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http://dx.doi.org/10.1007/s10565-020-09541-5DOI Listing

Methylsulfonylmethane sensitizes endometrial cancer cells to doxorubicin.

Cell Biol Toxicol 2020 Jun 20. Epub 2020 Jun 20.

Medical University of Lodz, Department of Cell Cultures and Genomic Analysis, Zeligowskiego 7/9, 90-752, Lodz, Poland.

Background: Methylsulfonylmethane (MSM) is a commonly used diet supplement believed to decrease the inflammation in joints and fastens recovery in osteoarthritis, gastric mucosal injury, or obesity-related disorders. It was also suggested that MSM might play a beneficial role in cancer treatment.

Purpose: So far, the MSM might have a potentially beneficial effect in endometrial cancer (EC) treatment. Read More

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http://dx.doi.org/10.1007/s10565-020-09542-4DOI Listing

Therapeutic targets during mitochondrial lipid metabolism.

Cell Biol Toxicol 2020 Jun 17;36(3):205-208. Epub 2020 Jun 17.

Zhongshan Hospital Institute for Clinical Science, Shanghai Institute of Clinical Bioinformatics, Shanghai Engineering Research for AI Technology for Cardiopulmonary Diseases, Fudan University, Shanghai, China.

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http://dx.doi.org/10.1007/s10565-020-09543-3DOI Listing

Drug-induced hepatic steatosis in absence of severe mitochondrial dysfunction in HepaRG cells: proof of multiple mechanism-based toxicity.

Cell Biol Toxicol 2020 Jun 14. Epub 2020 Jun 14.

INSERM, Univ Rennes, INRAE, Institut NUMECAN (Nutrition Metabolisms and Cancer) UMR_A 1341, UMR_S 1241, F-35000, Rennes, France.

Steatosis is a liver lesion reported with numerous pharmaceuticals. Prior studies showed that severe impairment of mitochondrial fatty acid oxidation (mtFAO) constantly leads to lipid accretion in liver. However, much less is known about the mechanism(s) of drug-induced steatosis in the absence of severe mitochondrial dysfunction, although previous studies suggested the involvement of mild-to-moderate inhibition of mtFAO, increased de novo lipogenesis (DNL), and impairment of very low-density lipoprotein (VLDL) secretion. Read More

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http://dx.doi.org/10.1007/s10565-020-09537-1DOI Listing

Human umbilical cord blood-derived MSCs exosome attenuate myocardial injury by inhibiting ferroptosis in acute myocardial infarction mice.

Cell Biol Toxicol 2020 Jun 13. Epub 2020 Jun 13.

Department of Cardiovascular Surgery, Henan Provincial People's Hospital and Fuwai Central China Cardiovascular Hospital, 1 Fuwai Road, Zhengzhou, Henan, 461464, People's Republic of China.

The exosome of MSCs derived from human umbilical cord blood (HUCB-MSC) has been reported to have cardioprotective effects on mouse models of acute myocardial infarction (AMI) and cardiomyocyte hypoxia injury, but the exact mechanisms involved require further investigation. This paper aimed to study the role of HUCB-MSC-exosomes in inhibiting ferroptosis to attenuate myocardial injury. Compared with sham or normoxia groups, RT-PCR and western blotting showed that divalent metal transporter 1 (DMT1) expression was significantly increased, and Prussian blue staining, ferrous iron (Fe), MDA, and GSH level detection demonstrated that ferroptosis occurred in the infraction myocardium and in cardiomyocyte following hypoxia-induced injury. Read More

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http://dx.doi.org/10.1007/s10565-020-09530-8DOI Listing

TRPV3 enhances skin keratinocyte proliferation through EGFR-dependent signaling pathways.

Cell Biol Toxicol 2020 Jun 13. Epub 2020 Jun 13.

State Key Laboratory of Natural Medicines and Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Development, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China.

Transient receptor potential vanilloid 3 (TRPV3) is highly expressed in skin keratinocytes where it forms Ca-permeable nonselective cation channels to regulate various cutaneous functions. TRPV3 expression is upregulated in many skin disorders. Here, we examined how TRPV3 affects keratinocyte proliferation and investigated the underlying mechanism. Read More

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http://dx.doi.org/10.1007/s10565-020-09536-2DOI Listing

A new sight: topology-dependent mitophagy.

Cell Biol Toxicol 2020 Jun 11;36(3):199-204. Epub 2020 Jun 11.

Guangzhou Regenerative Medicine and Health Guangdong Laboratory, CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Hefei Institute of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences; Guangzhou Medical University, Guangzhou, 510530, China.

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http://dx.doi.org/10.1007/s10565-020-09534-4DOI Listing

How to breakthrough mitochondrial DNA methylation-associated networks.

Cell Biol Toxicol 2020 Jun 8;36(3):195-198. Epub 2020 Jun 8.

Zhongshan Hospital Institute for Clinical Science, Shanghai Institute of Clinical Bioinformatics, Shanghai Engineering Research for AI Technology for Cardiopulmonary Diseases, Fudan University, Shanghai, China.

Mitochondrial DNA (mtDNA) plays an important role in regulating mitochondrial homeostasis, transcription, cell metabolism, and drug sensitivity. Patterns and regulations of mtDNA methylation vary among cell types, species, functions, and diseases. High-resolution mtDNA methylation maps of human and animal mitochondrial genomes addressed that the light (L)-strand non-CpG methylation of mtDNA varied among species, developing stages, and ages. Read More

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http://dx.doi.org/10.1007/s10565-020-09539-zDOI Listing

MSC encapsulation in alginate microcapsules prolongs survival after intra-articular injection, a longitudinal in vivo cell and bead integrity tracking study.

Cell Biol Toxicol 2020 May 30. Epub 2020 May 30.

Department of Orthopaedics, Erasmus MC University Medical Center Rotterdam, Wytemaweg 80, 3015, CN, Rotterdam, the Netherlands.

Mesenchymal stem cells (MSC) are promising candidates for use as a biological therapeutic. Since locally injected MSC disappear within a few weeks, we hypothesize that efficacy of MSC can be enhanced by prolonging their presence. Previously, encapsulation in alginate was suggested as a suitable approach for this purpose. Read More

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http://dx.doi.org/10.1007/s10565-020-09532-6DOI Listing

UCP1 regulates ALDH-positive breast cancer stem cells through releasing the suppression of Snail on FBP1.

Cell Biol Toxicol 2020 May 29. Epub 2020 May 29.

Key Laboratory of Breast Cancer in Shanghai, The Shanghai Key Laboratory of Medical Epigenetics, Key Laboratory of Medical Epigenetics and Metabolism, Shanghai Medical College, Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences, Cancer Institutes, Fudan University, Shanghai, 200032, China.

Uncoupling protein 1 (UCP1) has been implicated in ameliorating metabolic related disorders, of which most symptoms are risk factors for breast cancer. Here, we found that UCP1 was obviously downregulated in basal-like breast cancer (BLBC) and was positively correlated with improved survival. However, the underlying regulatory mechanisms remain largely unknown. Read More

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http://dx.doi.org/10.1007/s10565-020-09533-5DOI Listing

Single-cell transcriptomics uncovers potential marker genes of ochratoxin A-sensitive renal cells in an acute toxicity rat model.

Cell Biol Toxicol 2020 May 28. Epub 2020 May 28.

Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China.

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http://dx.doi.org/10.1007/s10565-020-09531-7DOI Listing

Concentration-dependent toxicogenomic changes of silver nanoparticles in hepatocyte-like cells derived from human induced pluripotent stem cells.

Cell Biol Toxicol 2020 May 24. Epub 2020 May 24.

Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD, 20708, USA.

The application of silver nanoparticles (AgNPs) in consumer products has been increasing rapidly over the past decades. Therefore, in vitro models capable of accurately predicting the toxicity of AgNPs are much needed. Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (iPSCs) represent an attractive alternative in vitro hepatotoxicity model. Read More

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http://dx.doi.org/10.1007/s10565-020-09529-1DOI Listing

Short-form RON (sf-RON) enhances glucose metabolism to promote cell proliferation via activating β-catenin/SIX1 signaling pathway in gastric cancer.

Cell Biol Toxicol 2020 May 12. Epub 2020 May 12.

Department of Medical Oncology and Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Recepteur d'origine nantais (RON) has been implicated in cell proliferation, metastasis, and chemoresistance of various human malignancies. The short-form RON (sf-RON) encoded by RON transcripts was overexpressed in gastric cancer tissues, but its regulatory functions remain illustrated. Here, we found that sf-RON promoted gastric cancer cell proliferation by enhancing glucose metabolism. Read More

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http://dx.doi.org/10.1007/s10565-020-09525-5DOI Listing

Cytoplasmic sirtuin 6 translocation mediated by p62 polyubiquitination plays a critical role in cadmium-induced kidney toxicity.

Cell Biol Toxicol 2020 May 11. Epub 2020 May 11.

School of Medicine, Chosun University, 309 Pilmundaero, Dong-gu, Gwangju, 501-759, South Korea.

Sirtuin 6 (Sirt6) is important for maintaining kidney homeostasis and function. Cd exposure increases the risk of developing kidney diseases. However, the role of Sirt6 in kidney disease mechanisms is unclear. Read More

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http://dx.doi.org/10.1007/s10565-020-09528-2DOI Listing

Pulsed laser deposition temperature effects on strontium-substituted hydroxyapatite thin films for biomedical implants.

Cell Biol Toxicol 2020 May 6. Epub 2020 May 6.

Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche (ISM-CNR), Via del Fosso del Cavaliere 100, 00133, Rome, Italy.

Substituting small molecule drugs with abundant and easily affordable ions may have positive effects on the way countless disease treatments are approached. The interest in strontium cation in bone therapies soared in the wake of the success of strontium ranelate in the treatment of osteoporosis. A new method for producing thin strontium-containing hydroxyapatite (Sr-HA, CaSr(PO)(OH)) films as coatings that render bioinert titanium implant bioactive is reported here. Read More

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http://dx.doi.org/10.1007/s10565-020-09527-3DOI Listing

Silver nanoparticles and silver ions cause inflammatory response through induction of cell necrosis and the release of mitochondria in vivo and in vitro.

Cell Biol Toxicol 2020 May 4. Epub 2020 May 4.

Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, No. 17, Block 3, Southern Renmin Road, Chengdu, Sichuan, 610041, People's Republic of China.

Owing to the excellent antibacterial and antiviral activity, silver nanoparticles have a widespread use in the food and pharmaceutical industries. With the increase in the production and use of the related products, the potential hazard of silver nanoparticles has aroused public attention. The main purpose of this study is to explore the toxicity of silver nanoparticles and induction of lung inflammation in vitro and in vivo. Read More

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http://dx.doi.org/10.1007/s10565-020-09526-4DOI Listing

Roles of acyl-CoA synthetase long-chain family member 5 and colony stimulating factor 2 in inhibition of palmitic or stearic acids in lung cancer cell proliferation and metabolism.

Cell Biol Toxicol 2020 Apr 28. Epub 2020 Apr 28.

Department of Respiratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.

Lung cancer is a heterogeneous and complex disease with the highest incidence and mortality rate. The present study aims at defining the lung cancer phenome specificity of lipidomic profiles, screening target lipid-dependent transcriptional alternations, identifying target lipid-associated target genes, and exploring molecular mechanisms. Lung cancer-specific and lung cancer subtype-specific target lipids palmitic acid (C16:0) and stearic acid (C18:0) were found as target lipids by integrating clinical phenomics, lipidomics, and transcriptomics and exhibited antiproliferative effects in sensitive cells. Read More

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http://dx.doi.org/10.1007/s10565-020-09520-wDOI Listing

Regulation of DNA methylation signatures on NF-κB and STAT3 pathway genes and TET activity in cigarette smoke extract-challenged cells/COPD exacerbation model in vitro.

Cell Biol Toxicol 2020 Apr 27. Epub 2020 Apr 27.

Laboratory of Pulmonary Immunotoxicology, Department of Environmental Toxicology, Southern University and A&M College, Baton Rouge, LA, 70813, USA.

Background: Chronic obstructive pulmonary disease (COPD) is a global health problem. Currently, there is a lack of knowledge about the pathobiology of this disease and available therapies are ineffective. Cigarette smoking is the leading cause of COPD; however, not all smokers develop COPD. Read More

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http://dx.doi.org/10.1007/s10565-020-09522-8DOI Listing

Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway.

Cell Biol Toxicol 2020 Apr 11. Epub 2020 Apr 11.

Department of Radiobiology, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.

A percentage of colorectal cancer (CRC) patients display low sensitivity to radiotherapy, which affects its therapeutic effect. Cancer cells DNA double-strand breaks (DSBs) repair capacity is crucial for radiosensitivity, but the roles of long noncoding RNAs (lncRNAs) in this process are largely uncharacterized. This study aims to explore whether lnc-RI regulates CRC cell growth and radiosensitivity by regulating the nonhomologous end-joining (NHEJ) repair pathway. Read More

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http://dx.doi.org/10.1007/s10565-020-09524-6DOI Listing

Roles of TP53 gene in the development of resistance to PI3K inhibitor resistances in CRISPR-Cas9-edited lung adenocarcinoma cells.

Cell Biol Toxicol 2020 Apr 2. Epub 2020 Apr 2.

Zhongshan Hospital Institute of Clinical Science, Fudan University Shanghai Medical School, Shanghai, China.

The mutation rates of tumor suppressor protein p53 gene (TP53) are high in lung adenocarcinoma and promote the development of acquired drug resistance. The present study evaluated the p53-dependent role in lung cancer cell sensitivity to PI3K-specific inhibitors, PI3K-associated inhibitors, PI3K-non-related inhibitors, and protein-based stimuli using designed p53 mutation. We found that the deletion of p53 key regions from amino acid 96 to 393 with the CRISPR-Cas9 altered multi-dimensional structure and sequencing of p53, probably leading the secondary changes in chemical structures and properties of PI3K subunit proteins or in interactions between p53 and PI3K isoform genes. Read More

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http://dx.doi.org/10.1007/s10565-020-09523-7DOI Listing

Novel fusion protein NGR-sIL-24 for targetedly suppressing cancer cell growth via apoptosis.

Cell Biol Toxicol 2020 Apr 1;36(2):179-193. Epub 2020 Apr 1.

Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.

Pro-apoptotic peptides have attracted much attention as promising anticancer agents due to their high activity. However, poor cellular uptake of the peptides is often associated with limited therapeutic application. Cell-penetrating homing peptides (CPHPs) were found to increase cell internalization as well as anticancer efficacy of the peptide conjugates. Read More

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http://dx.doi.org/10.1007/s10565-020-09519-3DOI Listing

The use of large animals to facilitate the process of MSC going from laboratory to patient-'bench to bedside'.

Cell Biol Toxicol 2020 Apr 23;36(2):103-114. Epub 2020 Mar 23.

Division of Trauma and Orthopaedic Surgery, Cambridge University, Cambridge, UK.

Large animal models have been widely used to facilitate the translation of mesenchymal stem cells (MSC) from the laboratory to patient. MSC, with their multi-potent capacity, have been proposed to have therapeutic benefits in a number of pathological conditions. Laboratory studies allow the investigation of cellular and molecular interactions, while small animal models allow initial 'proof of concept' experiments. Read More

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http://dx.doi.org/10.1007/s10565-020-09521-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196082PMC

Sodium butyrate modulates gut microbiota and immune response in colorectal cancer liver metastatic mice.

Cell Biol Toxicol 2020 Mar 14. Epub 2020 Mar 14.

Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Colorectal cancer (CRC) liver metastasis (CLM) is the leading death cause of CRC patients, but there is no satisfied approach to treat CLM. Gut microbiota plays a pivotal role in CRC initiation and development. Targeting dysbiosis of the gut microbiota might open up new opportunities for CLM treatment. Read More

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http://dx.doi.org/10.1007/s10565-020-09518-4DOI Listing

Estrogen receptor alpha (ERα)-mediated coregulator binding and gene expression discriminates the toxic ERα agonist diethylstilbestrol (DES) from the endogenous ERα agonist 17β-estradiol (E2).

Cell Biol Toxicol 2020 Feb 22. Epub 2020 Feb 22.

Division of Toxicology, Wageningen University and Research, PO Box 8000, 6700 EA, Wageningen, The Netherlands.

Diethylstilbestrol (DES) is a synthetic estrogen and proven human teratogen and carcinogen reported to act via the estrogen receptor α (ERα). Since the endogenous ERα ligand 17β-estradiol (E2) does not show these adverse effects to a similar extent, we hypothesized that DES' interaction with the ERα differs from that of E2. The current study aimed to investigate possible differences between DES and E2 using in vitro assays that detect ERα-mediated effects, including ERα-mediated reporter gene expression, ERα-mediated breast cancer cell (T47D) proliferation and ERα-coregulator interactions and gene expression in T47D cells. Read More

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http://dx.doi.org/10.1007/s10565-020-09516-6DOI Listing
February 2020

Glutathione S-transferase P influences the Nrf2-dependent response of cellular thiols to seleno-compounds.

Cell Biol Toxicol 2020 Feb 18. Epub 2020 Feb 18.

Department of Pharmaceutical Sciences, University of Perugia, Pole of Via del Giochetto, building B, 06126, Perugia, Italy.

Recent findings suggest a functional interaction of the drug resistance enzyme glutathione S-transferase P (GSTP) with the transcription factor Nrf2, a master regulator of the adaptive stress response to cellular electrophiles. The effect of this interaction on the metabolism and redox of cellular thiols was investigated in this study during the exposure to alkylating Se-compounds in murine embryonic fibroblasts (MEFs). GSTP1-1 gene ablation was confirmed to upregulate Nrf2 activity and to increase Cys uptake and the de novo biosynthesis of reduced glutathione (GSH) that was readily released in the extracellular medium together with other cellular thiols. Read More

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http://dx.doi.org/10.1007/s10565-020-09517-5DOI Listing
February 2020

Single-cell biomedicine: roles of single-cell nuclear elements.

Cell Biol Toxicol 2020 Feb 1;36(1):1-3. Epub 2020 Feb 1.

Department of Anesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Single-cell biomedicine, a new merging discipline of cell biology and medicine to improve the life quality of patients, gains high priority in cell biology and toxicology. Single-cell nuclear elements are specially focused and headlined to understand regulatory mechanisms by which transcriptional factors, DNA elements, and genome organization interact with each other and contribute to the developmental evolution and dynamic formation of genotoxicity. Cell molecular phenomics, clonal genotypes, and structural metamorphoses during the cell cycle at single-cell level provide deep insights to understanding mechanism-based bridges between cell biology and toxicology, between cell sensitive and resistance, and between chronic diseases and cancer. Read More

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http://dx.doi.org/10.1007/s10565-020-09515-7DOI Listing
February 2020

Regression of castration-resistant prostate cancer by a novel compound QW07 targeting androgen receptor N-terminal domain.

Cell Biol Toxicol 2020 Jan 30. Epub 2020 Jan 30.

East China Normal University and Shanghai Fengxian District Central Hospital Joint Center for Translational Medicine, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.

Androgen deprivation therapy (ADT) via surgical or chemical castration frequently fails to halt lethal castration-resistant prostate cancer (CRPC), which is induced by multiple mechanisms involving constitutive androgen receptor (AR) splice variants, AR mutation, and/or de novo androgen synthesis. The AR N-terminal domain (NTD) possesses most transcriptional activity and is proposed as a potential target for CRPC drug development. We constructed a screening system targeting AR-NTD transcription activity to screening a compound library and identified a novel small molecule compound named QW07. Read More

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http://dx.doi.org/10.1007/s10565-020-09511-xDOI Listing
January 2020

Pum2-Mff axis fine-tunes mitochondrial quality control in acute ischemic kidney injury.

Cell Biol Toxicol 2020 Jan 28. Epub 2020 Jan 28.

Medical School of Chinese PLA, Chinese PLA General Hospital, Beijing, China.

Mitochondrial fission factor (Mff) has been demonstrated to play a role in the activation of mitochondrial cleavage and mitochondrial death, denoting its role in the regulation of mitochondrial quality control. Recent evidence suggested that the mRNA translation of Mff is under the negative regulation by the RNA-binding protein Pumilio2 (Pum2). This study was designed to examine the role of Pum2 and Mff in the governance of mitochondrial quality control in a murine model of acute ischemic kidney injury. Read More

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http://dx.doi.org/10.1007/s10565-020-09513-9DOI Listing
January 2020
2.677 Impact Factor

The role of Caspase-1/GSDMD-mediated pyroptosis in Taxol-induced cell death and a Taxol-resistant phenotype in nasopharyngeal carcinoma regulated by autophagy.

Cell Biol Toxicol 2020 Jan 28. Epub 2020 Jan 28.

Department of Otolaryngology-Head Neck Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan Province, China.

Taxol has been widely used as a first-line chemotherapeutic agent for the treatment of advanced nasopharyngeal carcinoma (NPC). However, acquired drug resistance has caused great difficulties in clinical treatment. Pyroptosis is a newly discovered programmed cell death pathway, and Caspase-1 and gasdermin D (GSDMD) play key roles in driving canonical pyroptosis. Read More

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http://dx.doi.org/10.1007/s10565-020-09514-8DOI Listing
January 2020
2.677 Impact Factor

Correction to: novel biphenyl diester derivative AB-38b inhibits NLRP3 inflammasome through Nrf2 activation in diabetic nephropathy.

Cell Biol Toxicol 2020 Jan 22. Epub 2020 Jan 22.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221004, Jiangsu, China.

Unfortunately, there are some tiny errors in the data for Fig. 1a-c and Fig. 2a-e in the published online paper. Read More

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http://dx.doi.org/10.1007/s10565-020-09512-wDOI Listing
January 2020
2.677 Impact Factor

Effective targeting of the ubiquitin-like modifier NEDD8 for lung adenocarcinoma treatment.

Cell Biol Toxicol 2020 Jan 6. Epub 2020 Jan 6.

Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.

Protein neddylation, a process of conjugating neural precursor cell expressed, developmentally downregulated 8 (NEDD8) to substrates, plays a tumor-promoting role in lung carcinogenesis. Our previous study showed MLN4924, an inhibitor of NEDD8 activating enzyme (E1), significantly inhibits the growth of multiple cancer cells. However, resistance can develop to MLN4924 by mutation. Read More

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http://dx.doi.org/10.1007/s10565-019-09503-6DOI Listing
January 2020

LINC00858 promotes colorectal cancer by sponging miR-4766-5p to regulate PAK2.

Cell Biol Toxicol 2020 Jan 4. Epub 2020 Jan 4.

Department of Traditional Chinese Medicine, Guizhou Provincial People's Hospital, Zhongshan East Road 83, Guiyang, 550002, People's Republic of China.

Objectives: LncRNAs (long noncoding RNAs) have been reported to critically regulate colorectal cancer (CRC). We prospectively investigated effects and mechanisms of lncRNA LINC00858 on regulation of CRC progression.

Methods: Expression of LINC00858 and its target were analyzed by quantitative real-time polymerase chain reaction and in situ hybridization. Read More

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http://dx.doi.org/10.1007/s10565-019-09506-3DOI Listing
January 2020

Compound C induces autophagy and apoptosis in parental and hydroquinone-selected malignant leukemia cells through the ROS/p38 MAPK/AMPK/TET2/FOXP3 axis.

Cell Biol Toxicol 2020 Jan 3. Epub 2020 Jan 3.

Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.

Hydroquinone (HQ), a major metabolic product of benzene, causes acute myeloid leukemia (AML) elicited by benzene exposure. Past studies found that continuous exposure of human AML U937 cells to HQ selectively produces malignant U937/HQ cells in which FOXP3 upregulation modulates malignant progression. Other studies revealed that AMPK promotes TET2 activity on DNA demethylation and that TET2 activity is crucial for upregulating FOXP3 expression. Read More

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http://dx.doi.org/10.1007/s10565-019-09495-3DOI Listing
January 2020

Role of growth factors in hematopoietic stem cell niche.

Cell Biol Toxicol 2020 Apr 2;36(2):131-144. Epub 2020 Jan 2.

Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, South Korea.

Hematopoietic stem cells (HSCs) produce new blood cells everyday throughout life, which is maintained by the self-renewal and differentiation ability of HSCs. This is not controlled by the HSCs alone, but rather by the complex and exquisite microenvironment surrounding the HSCs, which is called the bone marrow niche and consists of various bone marrow cells, growth factors, and cytokines. It is essential to understand the characteristic role of the stem cell niche and the growth factors in the niche formation. Read More

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http://dx.doi.org/10.1007/s10565-019-09510-7DOI Listing
April 2020
2.677 Impact Factor

Evidence for the critical role of the PI3K signaling pathway in particulate matter-induced dysregulation of the inflammatory mediators COX-2/PGE and the associated epithelial barrier protein Filaggrin in the bronchial epithelium.

Cell Biol Toxicol 2019 Dec 28. Epub 2019 Dec 28.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Particulate matter (PM) is an environmental pollutant closely associated with human airway inflammation. However, the molecular mechanisms of PM-related airway inflammation remains to be fully elucidated. It is known that COX-2/PGE play key roles in the pathogenesis of airway inflammation. Read More

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http://dx.doi.org/10.1007/s10565-019-09508-1DOI Listing
December 2019

Correction to: Protein and lipid homeostasis altered in rat macrophages after exposure to metallic oxide nanoparticles.

Cell Biol Toxicol 2019 Dec 28. Epub 2019 Dec 28.

Institut Jean Lamour, UMR 7198, CNRS-Université de Lorraine, 2 allée André Guinier, BP 50840, 54011, Nancy, France.

Unfortunately, the author names in the author group section were incorrectly captured in the published online paper. Read More

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http://dx.doi.org/10.1007/s10565-019-09507-2DOI Listing
December 2019

BSA-bounded p-cresyl sulfate potentiates the malignancy of bladder carcinoma by triggering cell migration and EMT through the ROS/Src/FAK signaling pathway.

Cell Biol Toxicol 2019 Dec 24. Epub 2019 Dec 24.

Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University, 1-1 Jen-Ai Road, 10051, Taipei, Taiwan.

Para-cresyl sulfate (P-CS), a major uremic toxin derived from the metabolites of tyrosine and phenylalanine through liver, existed in the blood of patients with chronic kidney disease (CKD). CKD increases the malignancy in bladder cancers; however, effects of P-CS on bladder cancers are not fully understood. P-CS is conjugated with BSA physiologically, and this study aims to investigate the effects and possible underlying mechanisms of BSA-bounded P-CS on human bladder cancer cells. Read More

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http://dx.doi.org/10.1007/s10565-019-09509-0DOI Listing
December 2019

Understanding off-target effects through hybridization kinetics and thermodynamics.

Cell Biol Toxicol 2020 Feb 10;36(1):11-15. Epub 2019 Dec 10.

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.

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http://dx.doi.org/10.1007/s10565-019-09505-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051922PMC
February 2020

Cell death mechanisms in eukaryotes.

Cell Biol Toxicol 2020 Apr 9;36(2):145-164. Epub 2019 Dec 9.

School of Biological Sciences, UM-DAE Centre for Excellence in Basic Sciences, University of Mumbai, Vidyanagari, Mumbai, 400098, India.

Like the organism they constitute, the cells also die in different ways. The death can be predetermined, programmed, and cleanly executed, as in the case of apoptosis, or it can be traumatic, inflammatory, and sudden as many types of necrosis exemplify. Nevertheless, there are a number of cell deaths-some of them bearing a resemblance to apoptosis and/or necrosis, and many, distinct from each-that serve a multitude of roles in either supporting or disrupting the homoeostasis. Read More

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http://dx.doi.org/10.1007/s10565-019-09496-2DOI Listing

Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity.

Authors:
Litao Yan Xing Wu

Cell Biol Toxicol 2020 Apr 9;36(2):165-178. Epub 2019 Dec 9.

Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, People's Republic of China.

Animal and clinical studies have shown that mesenchymal stem cells (MSCs) play an important role in cartilage repair. The therapeutic effect of mesenchymal stem cells based therapies has been increasingly demonstrated to exosome-mediated paracrine secretion. Here, we investigated the cellular processes and mechanism of exosomes produced by conventional 2D culture (2D-Exos) and exosomes produced from 3D culture (3D-Exos) of umbilical MSCs (U-MSCs) in a hollow-fiber bioreactor for the treatment of cartilage repair. Read More

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http://dx.doi.org/10.1007/s10565-019-09504-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196084PMC

Long-term treatment with arsenite activates HER1 and HER2 through upregulating EGF, TGFα, and HSP90 in a human uroepithelial cell line.

Cell Biol Toxicol 2020 Jun 26;36(3):279-284. Epub 2019 Nov 26.

Department of Occupational and Environmental Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, 110122, Shenyang, People's Republic of China.

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http://dx.doi.org/10.1007/s10565-019-09500-9DOI Listing

Novel biphenyl diester derivative AB-38b inhibits NLRP3 inflammasome through Nrf2 activation in diabetic nephropathy.

Cell Biol Toxicol 2020 Jun 25;36(3):243-260. Epub 2019 Nov 25.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221004, Jiangsu, China.

Inflammation reaction mediated by NLRP3 inflammasome and Nrf2-related oxidative stress are vital participants in the development of diabetic nephropathy (DN) and closely associated to kidney fibrosis. Nrf2, a known antioxidative transcription factor, has been reported to activate NLRP3 inflammasome through its downstream factors (HO-1, NQO1, etc.) recently. Read More

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http://dx.doi.org/10.1007/s10565-019-09501-8DOI Listing
June 2020
2.677 Impact Factor

Temozolomide induces activation of Wnt/β-catenin signaling in glioma cells via PI3K/Akt pathway: implications in glioma therapy.

Cell Biol Toxicol 2020 Jun 22;36(3):273-278. Epub 2019 Nov 22.

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.

Glioblastoma (GBM) is the most aggressive type of glioma. Temozolomide (TMZ) is currently the drug of choice used for post-operative chemotherapy of GBM. However, the presence of intrinsic and acquired resistance hinders the success of chemotherapy. Read More

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http://dx.doi.org/10.1007/s10565-019-09502-7DOI Listing

Down-regulation of aryl hydrocarbon receptor intensifies carcinogen-induced retinal lesion via SOCS3-STAT3 signaling.

Cell Biol Toxicol 2020 Jun 20;36(3):223-242. Epub 2019 Nov 20.

Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan.

The aryl hydrocarbon receptor (AHR) is a ligand-activated receptor that regulates the metabolism of several xenobiotics and participates in ocular inflammation. Although severe inflammation is a major risk of retinal damage, the underlying mechanism is not well established. In this study, to elucidate how AHR mediates inflammation homeostasis, we hypothesized that AHR expression may diminish during long-term exposure to benzo [a] pyrene (B [a]P), a carcinogen in cigarette smoke. Read More

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http://dx.doi.org/10.1007/s10565-019-09499-zDOI Listing

CRISPR off-targets: a question of context.

Cell Biol Toxicol 2020 Feb 16;36(1):5-9. Epub 2019 Nov 16.

Genomics Institute, University of California at Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA.

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http://dx.doi.org/10.1007/s10565-019-09497-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056574PMC
February 2020