16,466 results match your criteria Cell And Tissue Research[Journal]


Disabled-2: a positive regulator of the early differentiation of myoblasts.

Cell Tissue Res 2020 Jun 30. Epub 2020 Jun 30.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Lo Kwee-Seong Integrated Biomedical Sciences Building, Hong Kong SAR, China.

Dab2 is an adaptor protein and a tumor suppressor. Our previous study has found that Dab2 was expressed in early differentiating skeletal muscles in mouse embryos. In this study, we determined the role of Dab2 in the skeletal muscle differentiation using C2C12 myoblasts in vitro and Xenopus laevis embryos in vivo. Read More

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http://dx.doi.org/10.1007/s00441-020-03237-2DOI Listing

Rat corneal endothelial cell migration during wound repair on the basement membrane depends more on the PI-3K pathway than the cdc-42 pathway or actin stress fibers.

Cell Tissue Res 2020 Jun 21. Epub 2020 Jun 21.

Department of Biological Sciences, 310 Dodge Hall, 118 Library Dr., Oakland University, Rochester, MI, 48309-4476, USA.

Following a central transcorneal circular freeze injury, organ-cultured rat corneal endothelial cells surrounding the wound reorganize peripheral actin bands into stress fibers and migrate individually into the wound. To ascertain the significance of this rearrangement relative to morphological changes accompanying migration and wound repair, some tissues were incubated overnight in 4 μM TRITC-conjugated phalloidin to stabilize actin and prevent its reorganization. After a freeze injury to the endothelium tissues were histologically observed at 24 h post-wounding and demonstrated that despite a lack of actin organization, cells responding to the injury appeared morphologically similar to their control counterparts. Read More

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http://dx.doi.org/10.1007/s00441-020-03229-2DOI Listing

Co-expression of the neuropeptide proctolin and glutamate in the central nervous system, along mechanosensory neurons and leg muscle in Cupiennius salei.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Department of Biology, Saint Michael's College, One Winooski Park, Colchester, VT, 05349, USA.

Similar to hair cells in the mammalian cochlear system, mechanosensory neurons in the Central American wandering spider Cupiennius salei are strongly innervated by efferent fibers that originate from neurons whose somata are located in the central nervous system (CNS). In both the mammalian and arachnid systems, efferent fibers have been shown to co-express two or more transmitters; however, our understanding regarding co-transmission and how it affects sensory signal transduction and processing in these systems is only fragmentary. The spider model system is exceptionally suitable for this type of investigation due to the large size and easy accessibility of the sensory and efferent neurons in this system. Read More

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http://dx.doi.org/10.1007/s00441-020-03217-6DOI Listing

Regulation of TrkB cell surface expression-a mechanism for modulation of neuronal responsiveness to brain-derived neurotrophic factor.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Institute of Clinical Neurobiology, University Hospital Wuerzburg, 97080, Wuerzburg, Germany.

Neurotrophin signaling via receptor tyrosine kinases is essential for the development and function of the nervous system in vertebrates. TrkB activation and signaling show substantial differences to other receptor tyrosine kinases of the Trk family that mediate the responses to nerve growth factor and neurotrophin-3. Growing evidence suggests that TrkB cell surface expression is highly regulated and determines the sensitivity of neurons to brain-derived neurotrophic factor (BDNF). Read More

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http://dx.doi.org/10.1007/s00441-020-03224-7DOI Listing

Existence and distribution of Niemann-Pick type 2C (NPC2) in prawn reproductive tract and its putative role as a cholesterol modulator during sperm transit in the vas deferens.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Department of Anatomy, Faculty of Science, Mahidol University, Rama 6 Rd., Ratchathewi, Bangkok, 10400, Thailand.

Sequestering of cholesterol (CHO) is a hallmark molecular event that is known to be associated with sperm gaining their fertilizing ability in a broad array of animals. We have shown previously that the level of CHO declines in the Macrobrachium rosenbergii sperm membrane when they are migrating into the vas deferens, prompting us to search for CHO transporters, one of which is Niemann-Pick type 2C (NPC2), within the prawn male reproductive tract. Sequence comparison of MrNPC2 with other NPC2, from crustaceans to mammals, revealed its conserved features in the hydrophobic cavity with 3 amino acids forming a CHO lid that is identical in all species analyzed. Read More

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http://dx.doi.org/10.1007/s00441-020-03225-6DOI Listing

Kruppel-like factor 4 upregulates matrix metalloproteinase 13 expression in chondrocytes via mRNA stabilization.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka, 565-0871, Japan.

Matrix metalloproteinase 13 (MMP13) is indispensable for normal skeletal development and is also a principal proteinase responsible for articular joint pathologies. MMP13 mRNA level needs to be tightly regulated in both positive and negative manners to achieve normal development and also to prevent joint destruction. We showed previously that Kruppel-like factor 4 (KLF4) strongly induces the expression of members of the MMP family of genes including that for MMP13 in cultured chondrocytes. Read More

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http://dx.doi.org/10.1007/s00441-020-03228-3DOI Listing

Trafficking of newly synthesized surfactant protein B to the lamellar body in alveolar type II cells.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku-Uchinada, Kahoku-gun, Ishikawa, 920-0293, Japan.

Lung surfactant accumulates in the lamellar body (LB) via not only the secretory (anterograde) pathway but also the endocytic (retrograde) pathway. Our previous studies suggested that the major surfactant components, phosphatidylcholine and surfactant protein A take independent trafficking routes in alveolar type II cells. Thus, trafficking of surfactant protein B (SP-B), a major hydrophobic surfactant apoprotein, should be re-evaluated by a straightforward method. Read More

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http://dx.doi.org/10.1007/s00441-020-03232-7DOI Listing

Temporal groups of lineage-related neurons have different neuropeptidergic fates and related functions in the Drosophila melanogaster CNS.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Centro de Biología Molecular Severo Ochoa (CBMSO), C/Nicolas Cabrera 1, 28049, Madrid, Spain.

The central nervous system (CNS) of Drosophila is comprised of the brain and the ventral nerve cord (VNC), which are the homologous structures of the vertebrate brain and the spinal cord, respectively. Neurons of the CNS arise from neural stem cells called neuroblasts (NBs). Each neuroblast gives rise to a specific repertory of cell types whose fate is unknown in most lineages. Read More

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http://dx.doi.org/10.1007/s00441-020-03231-8DOI Listing

Ovarian development pattern and vitellogenesis of ridgetail white prawn, Exopalaemon carinicauda.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Shanghai Collaborative Innovation Centre for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.

The ridgetail white prawn Exopalaemon carinicauda has the potential to be used as a model organism in crustacean research because it has a transparent body, available draft genome, and short life cycle. However, their ovarian development pattern remains unclear under laboratory culture conditions. This study investigated the changes of ovarian external feature, ovarian histology, gonadosomatic index (GSI), and hepatosomatic index (HSI), as well as the expression and localization of vitellogenin in the ovary and the hepatopancreas during the first ovarian development cycle of E. Read More

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http://dx.doi.org/10.1007/s00441-020-03223-8DOI Listing

Glial cell line-derived neurotrophic factors (GFLs) and small molecules targeting RET receptor for the treatment of pain and Parkinson's disease.

Cell Tissue Res 2020 Jun 17. Epub 2020 Jun 17.

Institute of Biotechnology, HiLIFE, University of Helsinki, Viikinkaari 5D, 00014, Helsinki, Finland.

Rearranged during transfection (RET), in complex with glial cell line-derived (GDNF) family receptor alpha (GFRα), is the canonical signaling receptor for GDNF family ligands (GFLs) expressed in both central and peripheral parts of the nervous system and also in non-neuronal tissues. RET-dependent signaling elicited by GFLs has an important role in the development, maintenance and survival of dopamine and sensory neurons. Both Parkinson's disease and neuropathic pain are devastating disorders without an available cure, and at the moment are only treated symptomatically. Read More

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http://dx.doi.org/10.1007/s00441-020-03227-4DOI Listing

Aquaporin 1 affects pregnancy outcome and regulates aquaporin 8 and 9 expressions in the placenta.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, No. 109 Xueyuan Xi Road Wenzhou, Zhejiang, 325027, China.

To explore the effects of aquaporin (AQP) 1 on pregnancy outcome and the association between expression of AQP1 and other AQPs in the placenta and foetal membranes, the rate of copulatory plugs and pregnancy, amniotic fluid (AF) volume, osmolality and composition were determined in AQP1-knockout (AQP1) mice at different gestational days (GD). The expression and location of AQP1 and other AQPs in the placenta and foetal membranes of AQP1 mice, AQP1-siRNA transfected WISH cells and oligohydramnios patients were also detected. Compared to control mice, AQP1 mice exhibited reduced copulation plug and successful pregnancy rates, but these effects were accompanied by a larger AF volume and lower AF osmolality at late gestation. Read More

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http://dx.doi.org/10.1007/s00441-020-03221-wDOI Listing

Hedgehog signaling regulates the basement membrane remodeling during folliculogenesis in the neonatal mouse ovary.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.

In the mouse ovary, interactions between oocytes and somatic cells are essential for folliculogenesis and subsequent follicle development. The polyovular follicle (PF), which contains more than two oocytes in a follicle, can be induced in the neonatal mouse ovary when interactions between oocytes and somatic cells are disrupted by agents such as the potent synthetic estrogen diethylstilbestrol (DES) acting through estrogen receptor (ER) β. Hedgehog signaling is known to regulate granulosa cell proliferation, thecal cell differentiation, and follicle growth. Read More

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http://dx.doi.org/10.1007/s00441-020-03222-9DOI Listing

Correction to: Neuropilin-1 receptor in the rapid and selective estrogen-induced neurovascular remodeling of rat uterus.

Cell Tissue Res 2020 Jun 15. Epub 2020 Jun 15.

Laboratory of Cell Biology, Instituto de Investigaciones Biológicas, Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, ZP, Uruguay.

The article title of the original publication contains error for the term "estrogen" was captured twice. Read More

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http://dx.doi.org/10.1007/s00441-020-03230-9DOI Listing

BDNF signaling during the lifetime of dendritic spines.

Cell Tissue Res 2020 Jun 14. Epub 2020 Jun 14.

Division of Cellular Neurobiology, Zoological Institute, TU Braunschweig, Spielmannstr 7, 38106, Braunschweig, Germany.

Dendritic spines are tiny membrane specialization forming the postsynaptic part of most excitatory synapses. They have been suggested to play a crucial role in regulating synaptic transmission during development and in adult learning processes. Changes in their number, size, and shape are correlated with processes of structural synaptic plasticity and learning and memory and also with neurodegenerative diseases, when spines are lost. Read More

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http://dx.doi.org/10.1007/s00441-020-03226-5DOI Listing

CiRS-126 inhibits proliferation of ovarian granulosa cells through targeting the miR-21-PDCD4-ROS axis in a polycystic ovarian syndrome model.

Cell Tissue Res 2020 Jul 29;381(1):189-201. Epub 2020 May 29.

Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, No. 26 Daoqian Street, Gusu District, Suzhou, 215002, Jiangsu, China.

Polycystic ovarian syndrome (PCOS) is considered to be one of the most prevalent endocrine disorders affecting women of reproductive age. CiRS-126, an innovative circular microRNA, has previously been proven to be a promising miR-21 sponge. However, a proper understanding of the impact of ciRS-126 on PCOS is needed. Read More

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http://dx.doi.org/10.1007/s00441-020-03187-9DOI Listing

KIF3A regulates the Wnt/β-catenin pathway via transporting β-catenin during spermatogenesis in Eriocheir sinensis.

Cell Tissue Res 2020 May 26. Epub 2020 May 26.

The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.

The Wnt/β-catenin pathway participates in many important physiological events such as cell proliferation and differentiation in the male reproductive system. We found that Kinesin-2 motor KIF3A is highly expressed during spermatogenesis in Eriocheir sinensis; it may potentially promote the intracellular transport of cargoes in this process. However, only a few studies have focused on the relationship between KIF3A and the Wnt/β-catenin pathway in the male reproductive system of decapod crustaceans. Read More

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http://dx.doi.org/10.1007/s00441-020-03220-xDOI Listing

Morphology and composition of the midgut bacterial community of Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae).

Cell Tissue Res 2020 May 23. Epub 2020 May 23.

Departamento de Biologia Geral, Universidade Federal de Viçosa, Vicosa, Minas Gerais, 36570-000, Brazil.

The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbionts of the midgut of S. Read More

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http://dx.doi.org/10.1007/s00441-020-03197-7DOI Listing
May 2020
3.565 Impact Factor

Restoration of estrous cycles by co-transplantation of mouse ovarian tissue with MSCs.

Cell Tissue Res 2020 May 19. Epub 2020 May 19.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

This study investigates the effect of bone marrow (BM-MSCs) and visceral peritoneum (VP-MSCs)-derived mesenchymal stem cells on the transplanted ovary. VP-MSCs and BM-MSCs were obtained from green fluorescent protein-expressing mice (GFP). Six- to eight-week-old female NMRI mice were divided into four experimental groups, autograft ovarian tissue fragments (AO), autograft ovarian tissue fragments encapsulated in fibrin-collagen hydrogel (AO-H), autograft ovarian tissue fragments encapsulated in fibrin-collagen hydrogel containing BM-MSCs (AO-HB) and autograft ovarian tissue fragments encapsulated in fibrin-collagen hydrogel containing VP-MSCs (AO-HP). Read More

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http://dx.doi.org/10.1007/s00441-020-03204-xDOI Listing

Expression characteristics of immune factors in secondary lymphoid organs of newborn, juvenile and adult yaks (Bos grunniens).

Cell Tissue Res 2020 May 19. Epub 2020 May 19.

Laboratory of Animal Anatomy & Tissue Embryology, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.

Little is known about lymphoid organ development in yaks. In this study, we characterize and evaluate the main markers of T cell, B cell, plasma cell and antigen-presenting cell in the mesenteric lymph nodes, spleen and hemal node in newborn, juvenile and adult yaks by immunohistochemistry, real-time quantitative polymerase chain reaction and western blotting. The structures of all organs were not fully developed in newborn. Read More

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http://dx.doi.org/10.1007/s00441-020-03219-4DOI Listing
May 2020
3.565 Impact Factor

Gastric smooth muscle cells manifest an abnormal phenotype in Parkinson's disease rats with gastric dysmotility.

Cell Tissue Res 2020 May 19. Epub 2020 May 19.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing, 100069, China.

Gastroparesis is a common symptom in Parkinson's disease (PD) and whether any change occurs in gastric smooth muscle cells (SMCs) of PD patients is unclear. We previously reported that rats with bilateral substantia nigra lesions induced by 6-hydroxydopamine (6-OHDA), referred to as 6-OHDA rats, manifest typical gastroparesis. In the present study, we further investigate the underlying mechanism. Read More

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http://dx.doi.org/10.1007/s00441-020-03214-9DOI Listing

Morphological and phenotypical diversity of eosinophils in the rat ileum.

Cell Tissue Res 2020 May 19. Epub 2020 May 19.

Laboratory of Histophysiology, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.

Eosinophils are abundantly present in intestinal mucosa. However, the morphological characteristics of their cellular population are still largely unknown. In this study, we examine their characteristics in the rat ileal mucosa using histological and ultrastructural methods. Read More

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http://dx.doi.org/10.1007/s00441-020-03209-6DOI Listing

Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary.

Cell Tissue Res 2020 May 16. Epub 2020 May 16.

Faculty of Science, Saitama University, Saitama, Japan.

The anterior pituitary gland is composed of five types of hormone-producing cells and folliculo-stellate cells. Folliculo-stellate cells do not produce anterior pituitary hormones but they are thought to play important roles as stem cells, phagocytes, or supporting cells of hormone-producing cells in the anterior pituitary. S100β protein has been used as a folliculo-stellate cell marker in some animals, including rats. Read More

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http://dx.doi.org/10.1007/s00441-020-03200-1DOI Listing

The lack of terminal tubule cells in the submandibular gland of mice deficient in submandibular gland protein C.

Cell Tissue Res 2020 May 16. Epub 2020 May 16.

Department of Clinical Engineering, Faculty of Health Sciences, Komatsu University, Komatsu, Japan.

The submandibular gland (SMG) of newborn mice has no mature acini but has the rudiments of acini called terminal tubules (TT). The TT are composed of TT cells with dark secretory granules and proacinar cells with lighter secretory granules, the latter being considered the immediate precursor of mature acinar cells. TT cells contain a specific secretory protein, submandibular gland protein C (SMGC) and they decrease in number postnatally at a higher rate in males than in females. Read More

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http://dx.doi.org/10.1007/s00441-020-03205-wDOI Listing

Evaluation of alginate modification effect on cell-matrix interaction, mechanotransduction and chondrogenesis of encapsulated MSCs.

Cell Tissue Res 2020 May 13. Epub 2020 May 13.

National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.

Mesenchymal stem cells (MSCs) are promising cell candidates for cartilage regeneration. Furthermore, it is important to control the cell-matrix interactions that have a direct influence on cell functions. Providing an appropriate microenvironment for cell differentiation in response to exogenous stimuli is a critical step towards the clinical utilization of MSCs. Read More

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http://dx.doi.org/10.1007/s00441-020-03216-7DOI Listing

Live-cell imaging and ultrastructural analysis reveal remarkable features of cultured porcine gonocytes.

Cell Tissue Res 2020 May 9. Epub 2020 May 9.

Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, S7N 5B4, SK, Canada.

Gonocytes in the neonatal testis have male germline stem cell potential. The objective of the present study was to examine the behavior and ultrastructure of gonocytes in culture. Neonatal porcine testis cells were cultured for 4 weeks and underwent live-cell imaging to explore real-time interactions among cultured cells. Read More

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http://dx.doi.org/10.1007/s00441-020-03218-5DOI Listing
May 2020
3.565 Impact Factor

Region-specific immune responses to autoimmune epididymitis in the murine reproductive tract.

Cell Tissue Res 2020 May 8. Epub 2020 May 8.

Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Victoria, Australia.

Epididymitis is a common pathology of the male reproductive tract, potentially leading to infertility. Studies on bacterial epididymitis indicate that the cauda epididymis is more susceptible to inflammatory damage than the caput. These regional differences in immunoregulation are further investigated using an experimental autoimmune epididymo-orchitis model. Read More

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http://dx.doi.org/10.1007/s00441-020-03215-8DOI Listing

Inhibin A regulates follicular development via hormone secretion and granulosa cell behaviors in laying hens.

Cell Tissue Res 2020 May 6. Epub 2020 May 6.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.

Inhibin A regulates follicular development, and its expression level is related to physiological activities, such as the recruitment, selection, and predominance during follicular development. Therefore, examining inhibin A and its regulatory effects on the reproductive performance of poultry is crucial. In this study, we measured the mRNA and protein abundances of INHA and INHBA in the chicken reproductive system and determined the hormone secretion and apoptosis of follicular granulosa cells (GCs) after being treated with inhibin A protein, and flow cytometry was performed to analyze GC apoptosis in INHA-specific small RNA interference (siRNA). Read More

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http://dx.doi.org/10.1007/s00441-020-03207-8DOI Listing

An organoid model to assay the role of CFTR in the human epididymis epithelium.

Cell Tissue Res 2020 May 6. Epub 2020 May 6.

Department of Genetics and Genome Sciences, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.

Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. Read More

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http://dx.doi.org/10.1007/s00441-020-03208-7DOI Listing

Exosomal miR-9-5p secreted by bone marrow-derived mesenchymal stem cells alleviates osteoarthritis by inhibiting syndecan-1.

Cell Tissue Res 2020 Jul 6;381(1):99-114. Epub 2020 May 6.

Department of Orthopaedics, Dashiqiao Central Hospital, Shenyang, 115100, People's Republic of China.

Mesenchymal stem cells (MSCs) have been demonstrated to serve as targets for the treatment of osteoarthritis (OA) and exosomes derived from MSCs also display chondroprotective effects. This study aims to investigate the regulatory role of exosomal microRNA-9-5p (miR-9-5p) secreted by bone marrow-derived MSCs (BM-MSCs) on OA in a rat model induced by anterior cruciate ligament/medial collateral ligament transection. Luciferase reporter assay was conducted to verify the putative miR-9-5p binding sites to 3'UTR of syndecan-1 (SDC1). Read More

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http://dx.doi.org/10.1007/s00441-020-03193-xDOI Listing

The pig as a model for immunology research.

Authors:
Reinhard Pabst

Cell Tissue Res 2020 May 30;380(2):287-304. Epub 2020 Apr 30.

Institute of Immunomorphology, Centre of Anatomy, Medical School Hannover, Hanover, Germany.

The pig is an omnivorous, monogastric species with many advantages to serve as an animal model for human diseases. There are very high similarities to humans in anatomy and functions of the immune system, e g., the presence of tonsils, which are absent in rodents. Read More

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http://dx.doi.org/10.1007/s00441-020-03206-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223737PMC

Extracellular matrix gene expression during arm regeneration in Amphiura filiformis.

Cell Tissue Res 2020 Apr 30. Epub 2020 Apr 30.

Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London, WC1E 6BT,, UK.

Extracellular matrix (ECM) plays a dynamic role during tissue development and re-growth. Body part regeneration efficiency relies also on effective ECM remodelling and deposition. Among invertebrates, echinoderms are well known for their striking regenerative abilities since they can rapidly regenerate functioning complex structures. Read More

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http://dx.doi.org/10.1007/s00441-020-03201-0DOI Listing

Bone marrow mesenchymal stem cells combine with normothermic machine perfusion to improve rat donor liver quality-the important role of hepatic microcirculation in donation after circulatory death.

Cell Tissue Res 2020 Apr 29. Epub 2020 Apr 29.

Department of Organ Transplantation, Tianjin First Central Hospital, No. 24 Fukang Road, Nankai District, Tianjin, 300192, People's Republic of China.

Donation after circulatory death (DCD) can expand the donor pool effectively. A gap remains in outcome between DCD livers and living donor livers, warranting improved DCD liver quality and urgent resolution. Bone marrow mesenchymal stem cells (BMMSCs) can regulate immunity, participate in the anti-inflammatory response, and secrete cytokines. Read More

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http://dx.doi.org/10.1007/s00441-020-03202-zDOI Listing

A model of respiratory syncytial virus (RSV) infection of infants in newborn lambs.

Cell Tissue Res 2020 May 29;380(2):313-324. Epub 2020 Apr 29.

LambCure LLC, Corvallis, OR, USA.

Many animal models have been established for respiratory syncytial virus (RSV) infection of infants with the purpose of studying the pathogenesis, immunological response, and pharmaceutical testing and the objective of finding novel therapies and preventive measures. This review centers on a neonatal lamb model of RSV infection that has similarities to RSV infection of infants. It includes a comprehensive description of anatomical and immunological similarities between ovine and human lungs along with comparison of pulmonary changes and immune responses with RSV infection. Read More

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http://dx.doi.org/10.1007/s00441-020-03213-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223741PMC

The study and manipulation of spermatogonial stem cells using animal models.

Cell Tissue Res 2020 May 27;380(2):393-414. Epub 2020 Apr 27.

Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5B4, Canada.

Spermatogonial stem cells (SSCs) are a rare group of cells in the testis that undergo self-renewal and complex sequences of differentiation to initiate and sustain spermatogenesis, to ensure the continuity of sperm production throughout adulthood. The difficulty of unequivocal identification of SSCs and complexity of replicating their differentiation properties in vitro have prompted the introduction of novel in vivo models such as germ cell transplantation (GCT), testis tissue xenografting (TTX), and testis cell aggregate implantation (TCAI). Owing to these unique animal models, our ability to study and manipulate SSCs has dramatically increased, which complements the availability of other advanced assisted reproductive technologies and various genome editing tools. Read More

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http://dx.doi.org/10.1007/s00441-020-03212-xDOI Listing
May 2020
3.565 Impact Factor

Utility of spontaneous animal models of Alzheimer's disease in preclinical efficacy studies.

Authors:
Caroline J Zeiss

Cell Tissue Res 2020 May 27;380(2):273-286. Epub 2020 Apr 27.

Department of Comparative Medicine, Yale School of Medicine, New Haven, CT, 06520, USA.

Spontaneous animal models of Alzheimer's disease (AD) offer the potential to bridge the translational gulf between promising rodent studies and failed human clinical trials. In this review, the relationship between cell biology, neuropathology, clinical phenotype and biomarker progression in human AD is summarized. Genetically altered animals have provided key insights into the cell biology of AD and, together with emerging stem cell systems, remain the most effective means to disentangle the entwined mechanisms that underlie AD. Read More

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http://dx.doi.org/10.1007/s00441-020-03198-6DOI Listing

Establishment and functional characterization of a murine primary Sertoli cell line deficient of connexin43.

Cell Tissue Res 2020 Apr 23. Epub 2020 Apr 23.

Institute of Anatomy, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.

The Sertoli cell (SC) specific connexin43 (Cx43) knockout (SCCx43KO) mouse line is ideal to gain insight into the mechanistic gap junction formation in SC and the seminiferous epithelium. A method for developing primary SC cultures from these mice was established, validated and successfully characterized via polymerase chain reaction, immunohistochemistry, immunofluorescence (IF), and Western blots (WB). It was evident that both knockout (KO) and wild-type (WT) primary cell cultures were similar in morphology. Read More

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http://dx.doi.org/10.1007/s00441-020-03203-yDOI Listing

Animal models to study the role of pulmonary intravascular macrophages in spontaneous and induced acute pancreatitis.

Cell Tissue Res 2020 May 22;380(2):207-222. Epub 2020 Apr 22.

Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, T2N 4Z6, Canada.

Acute necrotizing pancreatitis (ANP) is a common gastrointestinal cause of emergency admissions in dogs and humans and can lead to a systemic inflammatory response syndrome resulting in multiple organ dysfunction syndrome. Among the various complications associated with ANP, acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) is a major contributor leading to high mortality rates associated with severe acute pancreatitis (AP) in human patients. The incidence of ALI/ARDS in ANP dogs is not well-characterized in spontaneous AP and there are no models to study it in rodent models. Read More

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http://dx.doi.org/10.1007/s00441-020-03211-yDOI Listing

Innovative use of animal models to advance scientific research.

Cell Tissue Res 2020 05;380(2):205-206

Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.

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http://dx.doi.org/10.1007/s00441-020-03210-zDOI Listing

Transient lamellipodia predict sites of dendritic branch formation in hippocampal neurons.

Cell Tissue Res 2020 Jul 7;381(1):35-42. Epub 2020 Apr 7.

Department of Biology, Whitman College, 345 Boyer Ave, Walla Walla, WA, 99362, USA.

Extensive branching creates the complex dendritic arbor of mammalian CNS neurons but capturing the complete process of branch formation with time-lapse recordings has been challenging. Here, we report that application of BMP7 to cultured hippocampal neurons accelerated dendritic growth sufficiently to document branches forming in less than 20 h via frequent time-lapse imaging (10-min intervals). In these recordings, most branches emerged as collateral sprouts from the shaft of a parent branch. Read More

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http://dx.doi.org/10.1007/s00441-020-03194-wDOI Listing

BXSB/MpJ-Yaa mouse model of systemic autoimmune disease shows increased apoptotic germ cells in stage XII of the seminiferous epithelial cycle.

Cell Tissue Res 2020 Jul 4;381(1):203-216. Epub 2020 Apr 4.

Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo, Japan.

In mammals, the reproductive system and autoimmunity regulate mutual functions. Importantly, systemic autoimmune diseases are thought to cause male infertility but the underlying pathological mechanism remains unclear. In this study, the morpho-function of the testes in BXSB/MpJ-Yaa mice was analyzed as a representative mouse model for systemic autoimmune diseases to investigate the effect of excessive autoimmunity on spermatogenesis. Read More

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http://dx.doi.org/10.1007/s00441-020-03190-0DOI Listing

Functional morphology of the primary olfactory centers in the brain of the hermit crab Coenobita clypeatus (Anomala, Coenobitidae).

Cell Tissue Res 2020 Jun 2;380(3):449-467. Epub 2020 Apr 2.

Zoological Institute and Museum, Department of Cytology and Evolutionary Biology, University of Greifswald, Soldmannstrasse 23, 17498, Greifswald, Germany.

Terrestrial hermit crabs of the genus Coenobita display strong behavioral responses to volatile odors and are attracted by chemical cues of various potential food sources. Several aspects of their sense of aerial olfaction have been explored in recent years including behavioral aspects and structure of their peripheral and central olfactory pathway. Here, we use classical histological methods and immunohistochemistry against the neuropeptides orcokinin and allatostatin as well as synaptic proteins and serotonin to provide insights into the functional organization of their primary olfactory centers in the brain, the paired olfactory lobes. Read More

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http://dx.doi.org/10.1007/s00441-020-03199-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242284PMC

Neuropilin-1 receptor in the rapid and selective estrogen-induced neurovascular remodeling of rat uterus.

Cell Tissue Res 2020 Apr 2. Epub 2020 Apr 2.

Laboratory of Cell Biology, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, ZP 11600, Montevideo, Uruguay.

Sympathetic nerves innervate most organs and regulate organ blood flow. Specifically, in the uterus, estradiol (E2) elicits rapid degeneration of sympathetic axons and stimulates the growth of blood vessels. Both physiological remodeling processes, critical for reproduction, have been extensively studied but as independent events and are still not fully understood. Read More

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http://dx.doi.org/10.1007/s00441-020-03196-8DOI Listing

The roles of neuron-NG2 glia synapses in promoting oligodendrocyte development and remyelination.

Cell Tissue Res 2020 Jul 31;381(1):43-53. Epub 2020 Mar 31.

Department of Physiology, Army Medical University (Third Military Medical University), Chongqing, 400038, China.

NG2 immunopositive progenitor cells, also simply termed as NG2 glia and thought mainly to be oligodendrocyte precursor cells (OPCs), form synaptic connections with neurons in gray and white matters of brain. One of the most classical features of oligodendrocyte lineage cells is myelination, which will favor neuronal signaling transmission. Thus, is there a causal link between the specific synapses of neuron-NG2 glia and myelination? Building on this, here, we will discuss several relevant issues. Read More

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http://dx.doi.org/10.1007/s00441-020-03195-9DOI Listing

The role of Piezo proteins and cellular mechanosensing in tuning the fate of transplanted stem cells.

Cell Tissue Res 2020 Jul 25;381(1):1-12. Epub 2020 Mar 25.

INSERM U1148, Laboratory for Vascular Translational Science, Cardiovascular Bioengineering, Université Sorbonne Paris Nord, 93430, Villetaneuse, France.

Differentiation of stem cells can be modulated by a combination of internal and external signals, including mechanical cues from the surrounding microenvironment. Although numerous chemical and biological agents have been recognized in regulating stem cells' fate, little is known about their potential to directly sense the mechanical signals to choose differentiation into a specific lineage. The success of any stem cell transplantation effort, however, hinges on thorough understanding of the fate of these cells under different signals, including mechanical cues. Read More

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http://dx.doi.org/10.1007/s00441-020-03191-zDOI Listing

Sensory nerve endings arising from single spinal afferent neurons that innervate both circular muscle and myenteric ganglia in mouse colon: colon-brain axis.

Cell Tissue Res 2020 Jul 25;381(1):25-34. Epub 2020 Mar 25.

Visceral Neurophysiology Laboratory, College of Medicine and Public Health, Centre for Neuroscience, Flinders University, Bedford Park, Adelaide, South Australia, 5042, Australia.

There is considerable interest in understanding how contents within the gut wall (including microbiome) can activate sensory nerve endings in the gut that project to the central nervous system. However, we have only recently begun to understand the location and characteristics of extrinsic spinal afferent nerve endings that innervate the lower gastrointestinal (GI) tract. Our aim is to identify the nerve endings in the mouse distal colon that arise from single spinal afferent neurons. Read More

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http://dx.doi.org/10.1007/s00441-020-03192-yDOI Listing

FERM domain-containing protein 6 identifies a subpopulation of varicose nerve fibers in different vertebrate species.

Cell Tissue Res 2020 Jul 21;381(1):13-24. Epub 2020 Mar 21.

Institute of Anatomy and Cell Biology, University of Erlangen, Krankenhausstr. 9, 91054, Erlangen, Germany.

FERM domain-containing protein 6 (FRMD6) is a member of the FERM protein superfamily, which is evolutionary highly conserved and has recently been identified as an upstream regulator of the conserved growth-promoting Hippo signaling pathway. In clinical studies, the FRMD6 gene is correlated with high significance to Alzheimer's disease and cognitive impairment implicating a wider role of this protein in the nervous system. Scare data are available on the localization of endogenous FRMD6 in neural tissues. Read More

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http://dx.doi.org/10.1007/s00441-020-03189-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306050PMC

Inhibition of oxygen-sensing prolyl hydroxylases increases lipid accumulation in human primary tubular epithelial cells without inducing ER stress.

Cell Tissue Res 2020 Jul 18;381(1):125-140. Epub 2020 Mar 18.

Department of Nephrology and Hypertension, Friedrich-Alexander University Erlangen-Nürnberg and University Hospital Erlangen, Loschgestrasse 8, 91054, Erlangen, Germany.

The role of the hypoxia-inducible transcription factor (HIF) pathway in renal lipid metabolism is largely unknown. As HIF stabilizing prolyl hydroxylase (PHD) inhibitors are currently investigated in clinical trials for the treatment of renal anemia, we studied the effects of genetic deletion and pharmacological inhibition of PHDs on renal lipid metabolism in transgenic mice and human primary tubular epithelial cells (hPTEC). Tubular cell-specific deletion of HIF prolyl hydroxylase 2 (Phd2) increased the size of Oil Red-stained lipid droplets in mice. Read More

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http://dx.doi.org/10.1007/s00441-020-03186-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306052PMC

Regulation of Nrf2 and NF-κB during lead toxicity in bovine granulosa cells.

Cell Tissue Res 2020 Jun 18;380(3):643-655. Epub 2020 Feb 18.

Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.

Lead (Pb), one of the pervasive and protracted environmental heavy metals, is believed to affect the female reproductive system in many species. The Nrf2 and NF-κB are the two key transcriptional factors regulating cellular redox status and response against stress and inflammation respectively, showing an interaction between each other. The aim of this study is to investigate the effect of Pb on bovine granulosa cells (GCs) and its association with the regulation of Nrf2 and NF-κB pathways. Read More

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http://dx.doi.org/10.1007/s00441-020-03177-xDOI Listing
June 2020
3.565 Impact Factor

The association between increasing levels of O-GlcNAc and galectins in the liver tissue of hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus).

Cell Tissue Res 2020 Jul 10;381(1):115-123. Epub 2020 Mar 10.

Department of Biology, The University of Western Ontario, 1151 Richmond St. N, London, ON, N6A 5B7, Canada.

Post-translational glycosylation of proteins with O-linked β-N-acetylglucosamine (O-GlcNAcylation) and changes of galectin expression profiles are essential in many cellular stress responses. We examine this regulation in the liver tissue of hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus) representing a biological model of hypometabolism and physiological stress resistance. The tissue levels of O-GlcNAcylated proteins as well as galectin-1 and galectin-3 proteins detected by immunodot blot assay were significantly lower by 4. Read More

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http://dx.doi.org/10.1007/s00441-020-03185-xDOI Listing

Evidence that TNF-β suppresses osteoblast differentiation of mesenchymal stem cells and resveratrol reverses it through modulation of NF-κB, Sirt1 and Runx2.

Cell Tissue Res 2020 Jul 6;381(1):83-98. Epub 2020 Mar 6.

Musculoskeletal Research Group and Tumour Biology, Chair of Vegetative Anatomy, Faculty of Medicine, Institute of Anatomy, Ludwig-Maximilian-University Munich, Pettenkoferstrasse 11, 80336, Munich, Germany.

It has been established that inflammation plays an important role in bone formation and bone loss. Although a lot is known about the role of TNF-α in bone health, very little is understood about TNF-β, also called lymphotoxin. In this report, we examine the effect of TNF-β on osteogenic differentiation of mesenchymal stem cells (MSCs) and its modulation by resveratrol. Read More

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http://dx.doi.org/10.1007/s00441-020-03188-8DOI Listing