207,992 results match your criteria Cell Adhesion & Migration[Journal]


Effects of 17 α-methyltestosterone on the transcriptome, gonadal histology and sex steroid hormones in Pseudorasbora parva.

Theriogenology 2020 Jun 23;155:88-97. Epub 2020 Jun 23.

College of Animal Science and Technology, Shanxi Agricultural University, No. 1 Mingxiannan Road, Taigu, Jinzhong, Shanxi, 030801, China. Electronic address:

In recent years, the increasing use of environmental endocrine disruptors has caused serious environmental pollution and hurt aquatic organisms. It is still risky for aquatic species and humans exposed to 17α-methyltestosterone (MT), however, the harmful effect of MT on fish is still poorly understood. The main purpose of this study is to evaluate the influence of MT on Pseudorasbora parva at multi-levels. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2020.05.035DOI Listing

Molecular pathogenesis of the hyaluronic acid capsule of Pasteurella multocida.

Microb Pathog 2020 Jul 6:104380. Epub 2020 Jul 6.

Laboratory of Veterinary Biologics Engineering, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471023, China; Key-Disciplines Lab of Safety of Environment and Animal Product, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471023, China. Electronic address:

Pasteurella multocida possesses a viscous capsule polysaccharide on the cell surface, which is a critical structural component and virulence factor. Capsular polysaccharides are structurally similar to vertebrate glycosaminoglycans, providing an immunological mechanism for bacterial molecular mimicry, resistance to phagocytosis, and immune evasion during the infection process. Based on the capsular antigen, P. Read More

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http://dx.doi.org/10.1016/j.micpath.2020.104380DOI Listing

Everything Illuminated-Clostridium perfringens β-toxin.

Authors:
Wayne I Lencer

Cell Host Microbe 2020 Jul;28(1):5-6

Boston Children's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA; Harvard Digestive Disease Center, Boston, MA 02115, USA. Electronic address:

In this issue of Cell Host & Microbe, Bruggisser et al. show that Clostridium perfringens β-toxin (CPB) binds platelet endothelial cell adhesion molecule-1 (PECAM-1) (also known as CD31) to induce membrane pores. The discovery explains the cell type specificity for CPB and, in large part, the basic pathophysiology of disease. Read More

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http://dx.doi.org/10.1016/j.chom.2020.06.006DOI Listing

Cell-type-specific differences in KDEL receptor clustering in mammalian cells.

PLoS One 2020 9;15(7):e0235864. Epub 2020 Jul 9.

Molecular and Cell Biology, Department of Biosciences and Center of Human and Molecular Biology (ZHMB), Saarland University, Saarbrücken, Germany.

In eukaryotic cells, KDEL receptors (KDELRs) facilitate the retrieval of endoplasmic reticulum (ER) luminal proteins from the Golgi compartment back to the ER. Apart from the well-documented retention function, recent findings reveal that the cellular KDELRs have more complex roles, e.g. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235864PLOS

Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state.

PLoS Biol 2020 Jul 9;18(7):e3000755. Epub 2020 Jul 9.

School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

Kindlin-1, -2, and -3 directly bind integrin β cytoplasmic tails to regulate integrin activation and signaling. Despite their functional significance and links to several diseases, structural information on full-length kindlin proteins remains unknown. Here, we report the crystal structure of human full-length kindlin-3, which reveals a novel homotrimer state. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000755DOI Listing

Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis.

ACS Appl Mater Interfaces 2020 Jul 9. Epub 2020 Jul 9.

The application of cyclic strain is known to enhance myoblast differentiation and muscle growth and . However, current techniques apply strain to full tissues or cell monolayers, making it difficult to evaluate whether mechanical stimulation at the subcellular or single-cell scales would drive myoblast differentiation. Here, we report the use of optomechanical actuator (OMA) particles, comprised of a ~0. Read More

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http://dx.doi.org/10.1021/acsami.0c08871DOI Listing

The catalytically defective receptor protein tyrosine kinase EphA10 promotes tumorigenesis in pancreatic cancer cells.

Cancer Sci 2020 Jul 9. Epub 2020 Jul 9.

Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea.

EphA10 (erythropoietin-producing hepatocellular carcinoma receptor A10) is a catalytically defective receptor protein tyrosine kinase in the ephrin receptor family. Although EphA10 is involved in the malignancy of some types of cancer, its role as an oncogene has not been extensively studied. Here, we investigated the influence of EphA10 on the tumorigenic potential of pancreatic cancer cells. Read More

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http://dx.doi.org/10.1111/cas.14568DOI Listing

Association of Circulating Tumor DNA and Circulating Tumor Cells After Neoadjuvant Chemotherapy With Disease Recurrence in Patients With Triple-Negative Breast Cancer: Preplanned Secondary Analysis of the BRE12-158 Randomized Clinical Trial.

JAMA Oncol 2020 Jul 9. Epub 2020 Jul 9.

Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indianapolis.

Importance: A significant proportion of patients with early-stage triple-negative breast cancer (TNBC) are treated with neoadjuvant chemotherapy. Sequencing of circulating tumor DNA (ctDNA) after surgery, along with enumeration of circulating tumor cells (CTCs), may be used to detect minimal residual disease and assess which patients may experience disease recurrence.

Objective: To determine whether the presence of ctDNA and CTCs after neoadjuvant chemotherapy in patients with early-stage TNBC is independently associated with recurrence and clinical outcomes. Read More

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http://dx.doi.org/10.1001/jamaoncol.2020.2295DOI Listing

High-quality human preimplantation embryos stimulate endometrial stromal cell migration via secretion of microRNA hsa-miR-320a.

Hum Reprod 2020 Jul 9. Epub 2020 Jul 9.

Reproductive Biology Laboratory, Center for Reproductive Medicine, Amsterdam Reproduction & Development Research Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.

Study Question: How do high-quality human preimplantation embryos influence the endometrium to promote their own implantation?

Summary Answer: High-quality human preimplantation embryos secrete a specific microRNA (miRNA), hsa-miR-320a, which promotes migration of human endometrial stromal cells (hESCs).

What Is Known Already: We have previously shown that high-quality human preimplantation embryos excrete unknown factors that influence migration of hESCs.

Study Design, Size, Duration: Embryo excreted miRNAs, specifically those excreted by high-quality embryos, were identified and their effect on hESCs was determined by measuring the migration capacity and gene expression patterns of primary isolated hESCs. Read More

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http://dx.doi.org/10.1093/humrep/deaa149DOI Listing

In Vitro Immunotoxicity of Organophosphate Flame Retardants in Human THP-1-Derived Macrophages.

Environ Sci Technol 2020 Jul 9. Epub 2020 Jul 9.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Scarce attention has been paid to the immunotoxicity of organophosphate flame retardants (PFRs), which poses a challenge to the systematic assessment of their health risks. In this study, a battery of in vitro immunotoxicity screening assays, including adhesion, phagocytosis, and 48 cytokine/chemokine production, was measured after exposing THP-1-derived macrophages to six selected common PFRs (TPHP, TDCPP, TNBP, TOCP, TCEP, and TBOEP) at a noncytotoxic concentration (≤50 μM). Our results showed that TPHP and TBOEP partially attenuated the adhesion and phagocytosis of the THP-1 mφs and that TDCPP caused a functional loss of phagocytosis, implying the potential immunosuppression. Read More

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http://dx.doi.org/10.1021/acs.est.0c01152DOI Listing

Role of the small GTPase activating protein IQGAP1 in collagen phagocytosis.

J Cell Physiol 2020 Jul 9. Epub 2020 Jul 9.

Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.

Many adult connective tissues undergo continuous remodeling to maintain matrix homeostasis. Physiological remodeling involves the degradation of collagen fibers by the intracellular cathepsin-dependent phagocytic pathway. We considered that a multidomain, small GTPase activating protein, IQGAP1, which is involved in the generation of cell extensions, is required for collagen phagocytosis, possibly arising from its interactions with cdc42 and the actin-binding protein Flightless I (FliI). Read More

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http://dx.doi.org/10.1002/jcp.29933DOI Listing

Salivary and serum markers of angiogenesis in periodontitis in relation to smoking.

Clin Oral Investig 2020 Jul 8. Epub 2020 Jul 8.

Department of Periodontology, Faculty of Dentistry, University of Marmara, 34854 Maltepe, Istanbul, Turkey.

Objective: Angiogenesis is essential in maintenance of periodontal homeostasis, and it is regulated by growth factors and cytokines, including basic fibroblast growth factor (b-FGF), endoglin, platelet and endothelial cell adhesion molecule (PECAM-1), vascular endothelial growth factor (VEGF), soluble intercellular adhesion molecule-1 (sICAM-1), and soluble vascular cell adhesion molecule-1 (sVCAM-1). In this study, the salivary and serum concentrations of these angiogenesis-related proteins in relation to smoking and periodontitis were examined.

Material And Methods: Full-mouth periodontal status together with unstimulated whole saliva and serum samples was collected from 78 individuals, including 40 periodontitis patients (20 smokers and 20 nonsmokers) and 38 periodontally healthy controls (20 smokers and 18 nonsmokers). Read More

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http://dx.doi.org/10.1007/s00784-020-03411-4DOI Listing

Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.

J Cell Biol 2020 Sep;219(9)

Sorbonne Université, Institut National de la Santé et de la Recherche Médicale, Association Institut de Myologie, Centre de Recherche en Myologie, UMRS 974, Paris, France.

Clathrin function directly derives from its coat structure, and while endocytosis is mediated by clathrin-coated pits, large plaques contribute to cell adhesion. Here, we show that the alternative splicing of a single exon of the clathrin heavy chain gene (CLTC exon 31) helps determine the clathrin coat organization. Direct genetic control was demonstrated by forced CLTC exon 31 skipping in muscle cells that reverses the plasma membrane content from clathrin plaques to pits and by promoting exon inclusion that stimulated flat plaque assembly. Read More

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http://dx.doi.org/10.1083/jcb.201912061DOI Listing
September 2020

Expression profiling of the adhesion G protein-coupled receptor GPR133 (ADGRD1) in glioma subtypes.

Neurooncol Adv 2020 Jan-Dec;2(1):vdaa053. Epub 2020 Apr 28.

Departments of Neurosurgery, New York, New York, USA.

Background: Glioma is a family of primary brain malignancies with limited treatment options and in need of novel therapies. We previously demonstrated that the adhesion G protein-coupled receptor GPR133 (ADGRD1) is necessary for tumor growth in adult glioblastoma, the most advanced malignancy within the glioma family. However, the expression pattern of GPR133 in other types of adult glioma is unknown. Read More

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http://dx.doi.org/10.1093/noajnl/vdaa053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262742PMC

Circulating angiogenic cells in glioblastoma: toward defining crucial functional differences in CAC-induced neoplastic versus reactive neovascularization.

Neurooncol Adv 2020 Jan-Dec;2(1):vdaa040. Epub 2020 Apr 1.

Laboratory for Tumor Immuno-Pathology, Erasmus Medical Center, Rotterdam, The Netherlands.

Background: In order to identify suitable therapeutic targets for glioma anti-angiogenic therapy, the process of neovascularization mediated by circulating angiogenic cells (CACs) needs to be scrutinized.

Methods: In the present study, we compared the expression of neovascularization-related genes by 3 circulating CAC subsets (hematopoietic progenitor cells [HPCs], CD34, and KDR cells; internal controls: peripheral blood mononuclear cells and circulating endothelial cells) of treatment-naïve patients with glioblastoma (GBM) to those of patients undergoing reactive neovascularization (myocardial infarction (MI). CACs from umbilical cord (representing developmental neovascularization) and healthy subjects served as controls. Read More

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http://dx.doi.org/10.1093/noajnl/vdaa040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7276933PMC

The soluble form of pan-RTK inhibitor and tumor suppressor LRIG1 mediates downregulation of AXL through direct protein-protein interaction in glioblastoma.

Neurooncol Adv 2019 May-Dec;1(1):vdz024. Epub 2019 Sep 6.

NorLux Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, Luxembourg.

Background: Targeted approaches for inhibiting epidermal growth factor receptor (EGFR) and other receptor tyrosine kinases (RTKs) in glioblastoma (GBM) have led to therapeutic resistance and little clinical benefit, raising the need for the development of alternative strategies. Endogenous LRIG1 (Leucine-rich Repeats and ImmunoGlobulin-like domains protein 1) is an RTK inhibitory protein required for stem cell maintenance, and we previously demonstrated the soluble ectodomain of LRIG1 (sLRIG1) to potently inhibit GBM growth in vitro and in vivo.

Methods: Here, we generated a recombinant protein of the ectodomain of LRIG1 (sLRIG1) and determined its activity in various cellular GBM models including patient-derived stem-like cells and patient organoids. Read More

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http://dx.doi.org/10.1093/noajnl/vdz024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212925PMC
September 2019

Kupffer cells promote T-cell hepatitis by producing CXCL10 and limiting liver sinusoidal endothelial cell permeability.

Theranostics 2020 1;10(16):7163-7177. Epub 2020 Jun 1.

Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.

Kupffer cells (KCs) play a crucial role in liver immune homeostasis through interacting with other immune cells and liver sinusoidal endothelial cells (LSECs). However, how KCs exactly interact with these cells for maintaining the homeostasis still require the further investigation. CXCL10 is a chemokine that has been implicated in chemoattraction of monocytes, T cells, NK cells, and dendritic cells, and promotion of T cell adhesion to endothelial cells. Read More

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http://dx.doi.org/10.7150/thno.44960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330839PMC

DNA polymerase beta modulates cancer progression via enhancing CDH13 expression by promoter demethylation.

Oncogene 2020 Jul 8. Epub 2020 Jul 8.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.

DNA polymerase β (Pol β) plays a critical role in DNA base excision repair (BER), which is involved in maintaining genomic stability and in the modulation of DNA demethylation. Numerous studies implicated deficiency of Pol β in the genomic instability and dysregulation of genes expression, leading to affecting initiation of cancer. However, the role of Pol β in cancer progression is still unclear. Read More

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http://dx.doi.org/10.1038/s41388-020-1386-1DOI Listing

Identification of six candidate genes for endometrial carcinoma by bioinformatics analysis.

World J Surg Oncol 2020 Jul 8;18(1):161. Epub 2020 Jul 8.

Department of Gynaecology, The First Affiliated Hospital of Zhejiang Chinese Medical University; Zhejiang Provincial Hospital of Traditional Chinese Medicine, Youdian Road, Hangzhou, 310006, Zhejiang, China.

Background: Endometrial carcinoma (EC) is the most common gynecological malignant tumors which poses a serious threat to women health. This study aimed to screen the candidate genes differentially expressed in EC by bioinformatics analysis.

Methods: GEO database and GEO2R online tool were applied to screen the differentially expressed genes (DEGs) of EC from the microarray datasets. Read More

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http://dx.doi.org/10.1186/s12957-020-01920-wDOI Listing

Effects of two peroxide enzymatic denture cleaners on Candida albicans biofilms and denture surface.

BMC Oral Health 2020 Jul 8;20(1):193. Epub 2020 Jul 8.

Department of Periodontology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, PR China.

Objective: To compare the antifungal action of two commercially available denture cleaning agents to that of standard clinical solutions, and determine their effects on the polymethyl methacrylate (PMMA) acrylic resin denture surface.

Methods: Candida albicans growth was analyzed by colony forming assay, and the methyl thiazolyl tetrazolium (MTT) assay was used to evaluate biofilm formation and cell adhesion. The morphology and roughness of PMMA acrylic resin surface was measured by scanning electron microscopy (SEM) images and stylus method. Read More

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http://dx.doi.org/10.1186/s12903-020-01176-6DOI Listing

Mural Cell-Specific Deletion of Cerebral Cavernous Malformation 3 in the Brain Induces Cerebral Cavernous Malformations.

Arterioscler Thromb Vasc Biol 2020 Jul 9:ATVBAHA120314586. Epub 2020 Jul 9.

From the Interdepartmental Program in Vascular Biology and Therapeutics, Department of Pathology, Yale University School of Medicine, New Haven, CT. (K.W., H.Z., Y.H., Q.J., J.S.P., W.M., H.J.Z.).

Objective: Cerebral cavernous malformations (CCM), consisting of dilated capillary channels formed by a single layer of endothelial cells lacking surrounding mural cells. It is unclear why CCM lesions are primarily confined to brain vasculature, although the 3 CCM-associated genes (, , and ) are ubiquitously expressed in all tissues. We aimed to determine the role of gene in brain mural cell in CCM pathogenesis. Read More

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http://dx.doi.org/10.1161/ATVBAHA.120.314586DOI Listing

Importance of Initial Interfacial Steps during Chalcopyrite Bioleaching by a Thermoacidophilic Archaeon.

Microorganisms 2020 Jul 6;8(7). Epub 2020 Jul 6.

CINDEFI (CCT La Plata -CONICET, U.N.L.P.), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata 1900, Argentina.

Studies of thermophilic microorganisms have shown that they have a considerable biotechnological potential due to their optimum growth and metabolism at high temperatures. Thermophilic archaea have unique characteristics with important biotechnological applications; many of these species could be used in bioleaching processes to recover valuable metals from mineral ores. Particularly, bioleaching at high temperatures using thermoacidophilic microorganisms can greatly improve metal solubilization from refractory mineral species such as chalcopyrite (CuFeS), one of the most abundant and widespread copper-bearing minerals. Read More

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http://dx.doi.org/10.3390/microorganisms8071009DOI Listing

Sclerostin and Vascular Pathophysiology.

Int J Mol Sci 2020 Jul 6;21(13). Epub 2020 Jul 6.

Department of Clinical and Experimental Medicine, University of Messina, 98125 Messina, Italy.

There is cumulating evidence for a contribution of Wnt signaling pathways in multiple processes involved in atherosclerosis and vascular aging. Wnt signaling plays a role in endothelial dysfunction, in the proliferation and migration of vascular smooth muscle cells (VSMCs) and intimal thickening. Moreover, it interferes with inflammation processes, monocyte adhesion and migration, as well as with foam cell formation and vascular calcification progression. Read More

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http://dx.doi.org/10.3390/ijms21134779DOI Listing

Effect of sterilization treatment on mechanical properties, biodegradation, bioactivity and printability of GelMA hydrogels.

Biomed Mater 2020 Jul 8. Epub 2020 Jul 8.

Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, CANADA.

Gelatin methacryloyl (GelMA) hydrogel scaffolds and GelMA-based bioinks are widely used in tissue engineering and bioprinting due to their ability to support cellular functions and new tissue development. Unfortunately, while terminal sterilization of the GelMA is a critical step for translational tissue engineering applications, it can potentially cause thermal or chemical modifications of GelMA. Thus, understanding the effect of terminal sterilization on GelMA properties is an important, though often overlooked, aspect of material design for translational tissue engineering applications. Read More

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http://dx.doi.org/10.1088/1748-605X/aba40cDOI Listing

Single cell sequencing revealed the underlying pathogenesis of the development of osteoarthritis.

Gene 2020 Jul 5:144939. Epub 2020 Jul 5.

Wenzhou Center for Disease Control and Prevention, Wenzhou, China. Electronic address:

Osteoarthritis (OA) is a chronic degenerative change with high incidence and leads to a lower quality of life and a larger socioeconomic burden. This study aimed to explore potential crucial genes and pathways associated with OA that can be used as potential biomarkers forearly treatment. Single-cell gene expression profile of 1464 chondrocytes and 192 fibroblasts in OA were downloaded from the public database (GSE104782 and GSE109449) for subsequent analysis. Read More

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http://dx.doi.org/10.1016/j.gene.2020.144939DOI Listing

Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium.

Cell Rep 2020 Jul;32(1):107842

Faculty of Medicine, Department of Pediatrics, Gynecology & Obstetrics, University of Geneva, Geneva 1211, Switzerland; Faculty of Medicine, Department of Cell Physiology & Metabolism, University of Geneva, Geneva 1211, Switzerland. Electronic address:

Pseudomonas aeruginosa (Pa) represents the leading cause of airway infection in cystic fibrosis (CF). Early airways colonization can be explained by enhanced adhesion of Pa to the respiratory epithelium. RNA sequencing (RNA-seq) on fully differentiated primary cultures of airway epithelial cells from CF and non-CF donors predict that VAV3, β1 INTEGRIN, and FIBRONECTIN genes are significantly enriched in CF. Read More

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http://dx.doi.org/10.1016/j.celrep.2020.107842DOI Listing

Phosphoproteomic quantitation and causal analysis reveal pathways in GPVI/ITAM-mediated platelet activation programs.

Blood 2020 Jul 8. Epub 2020 Jul 8.

Oregon Health & Science University, Portland, Oregon, United States.

Platelets engage cues of pending vascular injury through coordinated adhesion, secretion and aggregation responses. These rapid, progressive changes in platelet form and function are orchestrated downstream of specific receptors on the platelet cell surface, and through intracellular signaling mechanisms that remain systematically undefined. This study brings together cell physiological and phosphoproteomics methods incorporating peptide tandem mass tag (TMT) labeling, sample multiplexing, synchronous precursor selection (SPS) and triple stage tandem mass spectrometry (MS3) to profile signaling mechanisms downstream of the immunotyrosine activation motif (ITAM) platelet collagen receptor GPVI. Read More

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http://dx.doi.org/10.1182/blood.2020005496DOI Listing

Degraded Porphyra yezoensis polysaccharide protects HK-2 cells and reduces nano-COM crystal toxicity, adhesion and endocytosis.

J Mater Chem B 2020 Jul 8. Epub 2020 Jul 8.

Department of Chemistry, Jinan University, Guangzhou 510632, China. and Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.

We studied the protection of degraded Porphyra yezoensis polysaccharide (PYP) on human proximal tubular epithelial cells (HK-2) from cytotoxicity of nano-calcium oxalate monohydrate (COM) crystal, and the regulation of adhesion and endocytosis of the COM crystal. Four degraded fractions, namely, PYP1, PYP2, PYP3, and PYP4, were successfully obtained, with molecular weights (Mws) of 576.2, 49. Read More

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http://dx.doi.org/10.1039/d0tb00360cDOI Listing

The role of adipokines in the modulation of lymphoid lineage cell development and activity: An overview.

Obes Rev 2020 Jul 7. Epub 2020 Jul 7.

Faculty of Health Sciences, Department of Experimental Immunology, Medical University of Lodz, Lodz, Poland.

Adipokines are predominantly known to play a vital role in the control of food intake, energy homeostasis and regulation of glucose and lipid metabolism. However, evidence supporting the concept of their extensive involvement in immune system defence mechanisms and inflammatory processes continues to grow. Some of the adipokines, that is, leptin and resistin, have been recognized to exhibit mainly pro-inflammatory properties, whereas others such as visfatin, chemerin, apelin and vaspin have been found to exert regulatory effects. Read More

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http://dx.doi.org/10.1111/obr.13055DOI Listing

Hydrogels with Cell Adhesion Peptide-Decorated Channel Walls for Cell Guidance.

Macromol Rapid Commun 2020 Jul 8:e2000295. Epub 2020 Jul 8.

Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Queensland, 4059, Australia.

A method is reported for making hollow channels within hydrogels decorated with cell-adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide-thiol chemistry, which are backfilled with a cysteine-containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell-guides, reducing the amount of required peptide 20-fold when compared to bulk functionalization. Read More

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http://dx.doi.org/10.1002/marc.202000295DOI Listing

Rab Proteins: Insights into Intracellular Trafficking in Endometrium.

Reprod Sci 2020 Jul 7. Epub 2020 Jul 7.

CONICET-UNCuyo-IMBECU, 5500, Mendoza, Argentina.

Rab proteins belong to the Ras superfamily of small monomeric GTPases. These G proteins are the main controllers of vesicular transport in every tissue, among them, the endometrium. They are in charge of to the functional subcellular compartmentalization and cargo transport between organelles and the plasma membrane. Read More

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http://dx.doi.org/10.1007/s43032-020-00256-wDOI Listing

Evaluation of the efficacy of platelet-rich plasma in preventing postoperative intraabdominal adhesions.

Turk J Surg 2020 Mar 18;36(1):53-58. Epub 2020 Mar 18.

Department of General Surgery, Selcuk University School of Medicine, Konya, Turkey.

Objectives: Postoperative intraabdominal adhesions still maintain their currency as serious causes of morbidity and mortality. This study aimed at evaluating the role of platelet-rich plasma (PRP) in the prevention of intraabdominal adhesions.

Material And Methods: A total of 16 healthy rabbits were used within the scope of the study. Read More

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http://dx.doi.org/10.5578/turkjsurg.4421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315448PMC

Osteoblast/fibroblast coculture derived bioactive ECM with unique matrisome profile facilitates bone regeneration.

Bioact Mater 2020 Dec 30;5(4):938-948. Epub 2020 Jun 30.

Zhejiang Key Laboratory of Pathophysiology, Medical School in Ningbo University, Ningbo, Zhejiang, PR China.

Extracellular matrix (ECM) with mimetic tissue niches was attractive to facilitate tissue regeneration via recruitment of endogenous cells and stimulation of self-healing process. However, how to engineer the complicate tissue specific ECM with unique matrisome was a challenge of ECM-based biomaterials in tissue engineering and regenerative medicine. Here, we introduced coculture system to engineer bone mimetic ECM niche guided by cell-cell communication. Read More

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http://dx.doi.org/10.1016/j.bioactmat.2020.06.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330453PMC
December 2020

biomineralization and biocompatibility of friction stir additively manufactured AZ31B magnesium alloy-hydroxyapatite composites.

Bioact Mater 2020 Dec 30;5(4):891-901. Epub 2020 Jun 30.

Laboratory for Laser Aided Additive and Subtractive Manufacturing, Department of Materials Science and Engineering, University of North Texas, 1150 Union Circle 305310, Denton, TX, 76203-5017, USA.

The present study aims to evaluate effect of hydroxyapatite (HA, Ca(PO)OH), a ceramic similar to natural bone, into AZ31B Mg alloy matrix on biomineralization and biocompatibility. The novel friction stir processing additive manufacturing route was employed to fabricate Mg-HA composites. Various HA contents (5, 10, 20 wt%) were incorporated into Mg matrix. Read More

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http://dx.doi.org/10.1016/j.bioactmat.2020.06.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332469PMC
December 2020

miR-7 Reduces Breast Cancer Stem Cell Metastasis via Inhibiting RELA to Decrease ESAM Expression.

Mol Ther Oncolytics 2020 Sep 4;18:70-82. Epub 2020 Jun 4.

Department of Pathogenic Biology and Immunology, School of Medicine, Southeast University, Nanjing 210009, China.

This study aimed to present evidence that miR-7 inhibited the metastasis of breast cancer stem cells (BCSCs) and elucidated the mechanisms that have remained unknown. The samples collected from miR-7 agomir-treated, BCSC-driven tumors were subjected to a protein array to analyze the protein expression profiles. A dual-luciferase reporter and chromatin immunoprecipitation-PCR were used to validate and evaluate the molecular expressions of interest in the collected breast cancer tissues and cell lines. Read More

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http://dx.doi.org/10.1016/j.omto.2020.06.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327889PMC
September 2020

Identification of the Combinatorial Effect of miRNA Family Regulatory Network in Different Growth Patterns of GC.

Mol Ther Oncolytics 2020 Jun 30;17:531-546. Epub 2020 Mar 30.

Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, China.

According to the growth pattern, gastric cancer (GC) could be classified into expanding-type GC and infiltrative-type GC (Ming's classification). The growth pattern of GC is often related to the malignant degree, invasion, metastasis, and other pathological characteristics of tumors. MicroRNAs (miRNAs) play important roles in modulating gene expression during the GC development. Read More

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http://dx.doi.org/10.1016/j.omto.2020.03.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321821PMC

Administration of Slow-Release Synthetic Prostacyclin Agonist Promoted Angiogenesis and Skeletal Muscle Regeneration for Limb Ischemia.

Mol Ther Methods Clin Dev 2020 Sep 22;18:119-130. Epub 2020 May 22.

Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.

Gene or cell therapy is currently not fully efficacious for arteriosclerosis obliterans (ASO). In this study, we determined whether YS-1402, a slow-release synthetic prostacyclin agonist, promoted neovascularization and skeletal muscle regeneration in a mouse model of critical limb ischemia (CLI). We ligated the femoral artery and its branches to obtain the CLI mouse model, administered saline (S group) or YS-1402 (YS group) to the thigh adductor 1 week after femoral artery occlusion, and evaluated tissue blood flow after surgery. Read More

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http://dx.doi.org/10.1016/j.omtm.2020.05.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321796PMC
September 2020

Osteoclast Signal Transduction During Bone Metastasis Formation.

Front Cell Dev Biol 2020 19;8:507. Epub 2020 Jun 19.

Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.

Osteoclasts are myeloid lineage-derived bone-resorbing cells of hematopoietic origin. They differentiate from myeloid precursors through a complex regulation process where the differentiation of preosteoclasts is followed by intercellular fusion to generate large multinucleated cells. Under physiological conditions, osteoclastogenesis is primarily directed by interactions between CSF-1R and macrophage colony-stimulating factor (M-CSF, CSF-1), receptor activator of nuclear factor NF-κB (RANK) and RANK ligand (RANKL), as well as adhesion receptors (e. Read More

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http://dx.doi.org/10.3389/fcell.2020.00507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317091PMC

Type III Secretion 1 Effector Gene Diversity Among Isolates From Coastal Areas in China.

Front Cell Infect Microbiol 2020 18;10:301. Epub 2020 Jun 18.

Department of Marine Biology, College of Oceanography, Hohai University, Nanjing, China.

Vibrios, which include more than 120 valid species, are an abundant and diverse group of bacteria in marine and estuarine environments. Some of these bacteria have been recognized as pathogens of both marine animals and humans, and therefore, their virulence mechanisms have attracted increasing attention. The type III secretion system (T3SS) is an important virulence determinant in many gram-negative bacteria, in which this system directly translocates variable effectors into the host cytosol for the manipulation of the cellular responses. Read More

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http://dx.doi.org/10.3389/fcimb.2020.00301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318850PMC

Evaluation of the Anti-Tumor Activity of the Humanized Monoclonal Antibody NEO-201 in Preclinical Models of Ovarian Cancer.

Front Oncol 2020 19;10:805. Epub 2020 Jun 19.

Women's Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

Despite high initial response rates with cytoreductive surgery, conventional chemotherapy and the incorporation of biologic agents, ovarian cancer patients often relapse and die from their disease. New approaches are needed to improve patient outcomes. This study was designed to evaluate the antitumor activity of NEO-201 monoclonal antibody (mAb) in preclinical models of ovarian cancer where the NEO-201 target is highly expressed. Read More

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http://dx.doi.org/10.3389/fonc.2020.00805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318110PMC

Paracrine SPARC signaling dysregulates alveolar epithelial barrier integrity and function in lung fibrosis.

Cell Death Discov 2020 30;6:54. Epub 2020 Jun 30.

Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, SO16 6YD UK.

Idiopathic pulmonary fibrosis (IPF) is a chronic scarring disease in which aging, environmental exposure(s) and genetic susceptibility have been implicated in disease pathogenesis, however, the causes and mechanisms of the progressive fibrotic cascade are still poorly understood. As epithelial-mesenchymal interactions are essential for normal wound healing, through human 2D and 3D in vitro studies, we tested the hypothesis that IPF fibroblasts (IPFFs) dysregulate alveolar epithelial homeostasis. Conditioned media from IPFFs exaggerated the wound-healing response of primary human Type II alveolar epithelial cells (AECs). Read More

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http://dx.doi.org/10.1038/s41420-020-0289-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327077PMC

Structural modeling, mutation analysis, and expression of usherin, a major protein in inherited retinal degeneration and hearing loss.

Comput Struct Biotechnol J 2020 10;18:1363-1382. Epub 2020 Jun 10.

Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah, Salt Lake City, UT, United States.

Usherin is the most common causative protein associated with autosomal recessive retinitis pigmentosa (RP) and Usher syndrome (USH), which are characterized by retinal degeneration alone and in combination with hearing loss, respectively. Usherin is essential for photoreceptor survival and hair cell bundle integrity. However, the molecular mechanism underlying usherin function in normal and disease conditions is unclear. Read More

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http://dx.doi.org/10.1016/j.csbj.2020.05.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317166PMC

HMGB1/PI3K/Akt/mTOR Signaling Participates in the Pathological Process of Acute Lung Injury by Regulating the Maturation and Function of Dendritic Cells.

Front Immunol 2020 19;11:1104. Epub 2020 Jun 19.

Department of Critical Care Medicine, Institute of Anesthesia and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

High-mobility group box 1 protein (HMGB1) was identified as a highly conserved DNA binding nuclear protein, which participates in the processes of acute lung injury (ALI). HMGB1 binds to its specific receptors not only to activate the nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) pathways but also to regulate the activation of the phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of the rapamycin (PI3K/AKT/mTOR) pathway. Mature dendritic cells (DCs) regulate acute lung inflammation and pathological injury in ALI. Read More

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http://dx.doi.org/10.3389/fimmu.2020.01104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318890PMC

Involvement of FAK/P38 Signaling Pathways in Mediating the Enhanced Osteogenesis Induced by Nano-Graphene Oxide Modification on Titanium Implant Surface.

Int J Nanomedicine 2020 30;15:4659-4676. Epub 2020 Jun 30.

Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, People's Republic of China.

Background: Titanium implants are widely used in dental and orthopedic medicine. Nevertheless, there is limited osteoinductive capability of titanium leading to a poor or delayed osseointegration, which might cause the failure of the implant therapy. Therefore, appropriate modification on the titanium surface for promoting osseointegration of existing implants is still pursued. Read More

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http://dx.doi.org/10.2147/IJN.S245608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335313PMC

BMP-1 disrupts cell adhesion and enhances TGF-β activation through cleavage of the matricellular protein thrombospondin-1.

Sci Signal 2020 Jul 7;13(639). Epub 2020 Jul 7.

University of Lyon, CNRS UMR 5305, Tissue Biology and Therapeutic Engineering Laboratory (LBTI), F-69367 Lyon, France.

Bone morphogenetic protein 1 (BMP-1) is an important metalloproteinase that synchronizes growth factor activation with extracellular matrix assembly during morphogenesis and tissue repair. The mechanisms by which BMP-1 exerts these effects are highly context dependent. Because BMP-1 overexpression induces marked phenotypic changes in two human cell lines (HT1080 and 293-EBNA cells), we investigated how BMP-1 simultaneously affects cell-matrix interactions and growth factor activity in these cells. Read More

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http://dx.doi.org/10.1126/scisignal.aba3880DOI Listing

A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly.

J Biol Chem 2020 Jul 6. Epub 2020 Jul 6.

Biological Sciences, University of Toledo, United States.

Nutrient-transporting enterocytes interact with their luminal environment using a densely-packed collection of apical microvilli known as the brush border.  Assembly of the brush border is controlled by the intermicrovillar adhesion complex (IMAC), a protocadherin-based complex found at the tips of brush border microvilli that mediates adhesion between neighboring protrusions.  ANKS4B is known to be an essential scaffold within the IMAC, though its functional properties have not been thoroughly characterized. Read More

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http://dx.doi.org/10.1074/jbc.RA120.013790DOI Listing

Collective cell migration and residual stress accumulation: Rheological consideration.

J Biomech 2020 Jul 13;108:109898. Epub 2020 Jun 13.

Faculty of Technology and Metallurgy, Belgrade University, Karnegijeva 4, Belgrade, Serbia.

Stress generation during collective cell migration represents one of the key factors which influence the configuration of migrating cells, viscoelasticity of multicellular systems and their inter-relation. Local generation of stress (normal and shear) is significant even in 2D. Normal stress is primarily accumulated within a core region of migrating cell clusters during their movement through the dense environment and during the collisions of migrating cell clusters caused by uncorrelated motility. Read More

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http://dx.doi.org/10.1016/j.jbiomech.2020.109898DOI Listing

Role of Curcumin and (-)-Epigallocatechin-3--Gallate in Bladder Cancer Treatment: A Review.

Cancers (Basel) 2020 Jul 5;12(7). Epub 2020 Jul 5.

Department of Pharmaceutical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.

The incidence of bladder cancer (BC) is increasing, and although current therapeutic approaches are effective in many cases, recurrence of BC is common. Therefore, it seems necessary to search not only for novel therapeutic approaches, but also for new therapeutic agents. Natural polyphenols, such as curcumin (CUR) and epigallocatechin gallate (EGCG), possess remarkable antitumor activity. Read More

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http://dx.doi.org/10.3390/cancers12071801DOI Listing

Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study.

J Clin Med 2020 Jul 4;9(7). Epub 2020 Jul 4.

Department of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Av. Gaspar Aguilar 90, 46017 Valencia, Spain.

Aim: The primary objective of this pilot study was to evaluate the effect of non-surgical periodontal treatment. The secondary aim was to evaluate the effect of dietary therapy on both parameters of oxidative stress in leukocytes and leukocyte-endothelial cell interactions in an obese population.

Methods: This was a pilot study with a before-and-after design. Read More

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http://dx.doi.org/10.3390/jcm9072117DOI Listing