6,559,342 results match your criteria Cell & Tissue Transplantation & Therapy [Journal]

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[Construction and analysis of gene co-expression networks in intracranial aneurysm].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(7):525-531

Department of Neurology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.

To analyze the expression microarray data in the public databases of intracranial aneurysms (IA) using bioinformatics, and to provide important information for the study of disease mechanisms. Gene co-expression network was constructed by weighted gene co-expression network analysis (WGCNA) based on the dataset (GSE75436) and pivot genes were identified. Using the online tool DAVID (Database for Annotation, Visualization, and Integrated Discovery) to perform GO function enrichment and KEGG path analysis on modules highly related to IA. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.07.010DOI Listing
February 2019

[Regulatory effect of exosomes secreted by vaginal wall fibroblasts on angiogenesis in patients with stress urinary incontinence].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(7):510-514

Department of Obstetrics & Gynecology, Shanxi Academy of Medical Sciences & Shanxi Da Yi Hospital, Shanxi Da Yi Hospital Affiliated to Shanxi Medical University, Taiyuan 030032, China.

Exosomes are considered to mediate intercellular communication by delivering biomolecules like mRNA, miRNA into recipient cells. The purpose of this study was to evaluate the effects of exosomes secreted by fibroblasts from women with stress urinary incontinence (SUI-EXO) on endothelial cells angiogenesis. Primary fibroblasts were acquired from periurethral vaginal wall tissues and exosomes were prepared by ultracentrifugation of fibroblasts cells conditioned medium. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.07.007DOI Listing
February 2019

[The effect of Cathepsin S in primary biliary cholangitis].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(7):505-509

Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Key Laboratory of Rheumatology and Cinical Immunology, Ministry of Education, Beijing 100730, China.

To explore the effect of cathepsin (Cat) S in primary biliary cholangitis (PBC). The serum of PBC patients and Hepatitis B virus (HBV) patients were collected in Peking Union Medical College Hospital from August 2016 to July 2017 and the liver tissue of PBC were collected from March 2010 to July 2013. Indirect enzyme-linked immunosorbent assay (ELISA) was used to detect the serum concentrations of total-and pro-Cat S respectively in 24 PBC patients, 24 Hepatitis B patients and 24 healthy controls. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.07.006DOI Listing
February 2019
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[Effects of penehyclidine hydrochloride on Nrf2/ARE signaling pathway during endotoxin-induced acute lung injury in neonate rats].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(6):453-457

Department of Anesthesiology, Affiliated Anti-cancer Hospital of Zhengzhou University, Zhengzhou 450008, China.

To evaluate the effects of penehyclidine hydrochloride on the nuclear factor erythroid 2-related factor 2/antioxidant responsive element (Nrf2/ARE) signaling pathway during endotoxin-induced acute lung injury(ALI) in neonate rats. Forty 7-day-old Wistar rats weighing 12-18 g were randomly divided into 4 groups (10) using a random number table: normal saline group(NS group), acute lung injury(ALI group), penehyclidine hydrochloride group(PHC group) and penehyclidine hydrochloride+ Nrf2 siRNA plasmid group(PNS group). The ALI model was induced with intraperitoneal endotoxin (5. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.06.014DOI Listing
February 2019
1 Read

[Establishment of rat model of cardiorenal syndrome by transabdominal subtotal nephrectomy].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(6):447-452

Department of Cardiovascular Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.

To evaluate the changes of rat renal function and cardiac function after transabdominal subtotal nephrectomy (STNx) and provide an ideal animal model for the study of chronic kidney disease (CKD) and cardiorenal syndrome (CRS). The SD rats (male, 5-6 weeks) were randomly divided into Sham group (10) and STNx group (10). The rats from Sham group underwent sham surgery, and the right nephrectomy plus the 2/3 left kidney artery ligation through abdominal incision were performed in the rats from STNx group. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.06.013DOI Listing
February 2019

[Activation of GPER inhibts proliferation and autophagy in prostatic epithelial cells].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(6):419-422

Department of Urology, the First Affiliated Hospital of Guangzhou Medical College, Guangdong Key Laboratory of Urology, Guangzhou 510230, China.

To investigate the effect of G protein-coupled estrogen receptor (GPER) on proliferation and autophagy in prostatic epithelial cells. Cell Counting Kit-8 (CCK8) assay was used to observe the growth of BPH-1 and RWPE-1 cells after treating with or not with estradiol or GPER selective agonist G1. Autophagy was quantified with Western blot and Cyto-ID autophagy detection kit after treating with estradiol, G1 or both G1 and G15 in the same cells. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.06.007DOI Listing
February 2019

Abdominal CT evaluation of the liver and spleen for staging mast cell tumors in dogs yields nonspecific results.

Vet Radiol Ultrasound 2019 Feb 20. Epub 2019 Feb 20.

Department of Clinical Sciences and Services, The Royal Veterinary College, North Mymms, Hatfield, AL9 7TA, UK.

Canine mast cell tumor staging is commonly performed using abdominal ultrasonography and fine-needle aspiration cytology of masses, lymph nodes, and hepatic and splenic parenchyma. Computed tomography is used for abdominal, thoracic, or whole body imaging in staging mast cell tumors in the authors' institution enabling evaluation of multiple body areas in one examination. The aim of this study was to compare the CT examinations acquired for staging of mast cell disease to their subsequent liver and spleen cytology findings. Read More

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http://dx.doi.org/10.1111/vru.12717DOI Listing
February 2019

Clonal expansion of hepatic progenitor cells and differentiation into hepatocyte-like cells.

Dev Growth Differ 2019 Feb 20. Epub 2019 Feb 20.

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Hepatic progenitor cells (HPCs) in adult liver are promising for treatment of liver diseases. A biliary-derived HPC population in adult mice has been characterized by co-expression of stem cell marker Sry (sex determining region Y)-box 9 (SOX9) and biliary marker cytokeratin 7 (CK7). However, isolation of these HPCs in adult healthy liver without any selection procedures remains a big challenge in this field. Read More

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http://doi.wiley.com/10.1111/dgd.12596
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http://dx.doi.org/10.1111/dgd.12596DOI Listing
February 2019
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Varicella Zoster Virus Induces Differential Cell-Type Specific Responses in Human Corneal Epithelial Cells and Keratocytes.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):704-711

Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, United States.

Purpose: While VZV DNA and antigen have been detected in acute and chronic VZV keratitis, it is unclear whether productive infection of corneal cells is ongoing or whether residual, noninfectious VZV antigens elicit inflammation. Herein, we examined VZV-infected primary human corneal epithelial cells (HCECs) and keratocytes (HKs) to elucidate the pathogenesis of VZV keratitis.

Methods: HCECs and HKs were mock- or VZV infected. Read More

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http://dx.doi.org/10.1167/iovs.18-25801DOI Listing
February 2019

Transformation of the Transcriptomic Profile of Mouse Periocular Mesenchyme During Formation of the Embryonic Cornea.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):661-676

BioSciences Department, Rice University, Houston, Texas, United States.

Purpose: Defects in neural crest development are a major contributing factor in corneal dysgenesis, but little is known about the genetic landscape during corneal development. The purpose of this study was to provide a detailed transcriptome profile and evaluate changes in gene expression during mouse corneal development.

Methods: RNA sequencing was used to uncover the transcriptomic profile of periocular mesenchyme (pNC) isolated at embryonic day (E) 10. Read More

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http://dx.doi.org/10.1167/iovs.18-26018DOI Listing
February 2019

KLF4 Regulates Corneal Epithelial Cell Cycle Progression by Suppressing Canonical TGF-β Signaling and Upregulating CDK Inhibitors P16 and P27.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):731-740

Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States.

Purpose: Krüppel-like factor 4 (KLF4) promotes corneal epithelial (CE) cell fate while suppressing mesenchymal properties. TGF-β plays a crucial role in cell differentiation and development, and if dysregulated, it induces epithelial-mesenchymal transition (EMT). As KLF4 and TGF-β regulate each other in a context-dependent manner, we evaluated the role of the crosstalk between KLF4 and TGF-β-signaling in CE homeostasis. Read More

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http://dx.doi.org/10.1167/iovs.18-26423DOI Listing
February 2019

3D bioprinted complex constructs reinforced by hybrid multilayers of electrospun nanofiber sheets.

Biofabrication 2019 Feb 20. Epub 2019 Feb 20.

Digital Manufacturing Process Group, Korea Institute of Industrial Technology, Siheung-si, KOREA, REPUBLIC OF.

Despite the usefulness of hydrogels for cell-based bioprinting, the fragility of their resulting constructs has hindered their practical applications in tissue engineering research. Here, we suggest a hybrid integration method based on cell-hydrogel bioprinting that includes alternate layering of flexible nanofiber sheets. Because the bioprinting was implemented on a nanofibrous surface, the hydrogel-based materials could be printed with enhanced shape resolution compared to printing on a bare hydrogel. Read More

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http://dx.doi.org/10.1088/1758-5090/ab08c2DOI Listing
February 2019

A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.

Biofabrication 2019 Feb 20. Epub 2019 Feb 20.

Center for Minimally Invasive Therapeutics, UCLA, Los Angeles, California, UNITED STATES.

Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly desirable. This work demonstrates a technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels by stacking and crosslinking prehydrogel solution in a layer by layer manner. Read More

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http://dx.doi.org/10.1088/1758-5090/ab08b5DOI Listing
February 2019

The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality.

Aging (Albany NY) 2019 Feb 20. Epub 2019 Feb 20.

State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, Jiangsu, P.R. China.

Tight control of energy metabolism is essential for normal cell function and organism survival. PKM (pyruvate kinase, muscle) isoforms 1 and 2 originate from alternative splicing of PKM pre-mRNA. They are key enzymes in oxidative phosphorylation and aerobic glycolysis, respectively, and are essential for ATP generation. Read More

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http://dx.doi.org/10.18632/aging.101802DOI Listing
February 2019

Continuous intracerebroventricular injection of Porphyromonas gingivalis lipopolysaccharide induces systemic organ dysfunction in a mouse model of Alzheimer's disease.

Exp Gerontol 2019 Feb 17. Epub 2019 Feb 17.

Department of Pharmacology and Molecular Therapeutics, Graduate School of Medical Sciences, Kumamoto University, Japan.

Systemic organ dysfunction is one of the important issues for the patients with Alzheimer's disease (AD) and their caregivers. Recent evidences suggest that periodontitis is a possible risk factor for progression of AD and lipopolysaccharide derived from Porphyromonas gingivalis (Pg-LPS) which is a major periodontopathic bacteria induces cognitive impairment in mice. However, the precise relationships between the brain exposure of Pg-LPS and systemic organ dysfunction in AD patients are still undetermined. Read More

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http://dx.doi.org/10.1016/j.exger.2019.02.007DOI Listing
February 2019

Cholinergic drugs ameliorate endothelial dysfunction by decreasing O-GlcNAcylation via M3 AChR-AMPK-ER stress signaling.

Life Sci 2019 Feb 17. Epub 2019 Feb 17.

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, People's Republic of China. Electronic address:

Aims: Obesity is associated with increased cardiovascular morbidity and mortality. It is accompanied by augmented O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins via increasing hexosamine biosynthetic pathway (HBP) flux. However, the changes and regulation of the O-GlcNAc levels induced by obesity are unclear. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.02.036DOI Listing
February 2019

An industry perspective: a streamlined screening strategy using alternative models for chemical assessment of developmental neurotoxicity.

Neurotoxicology 2019 Feb 17. Epub 2019 Feb 17.

Toxicology & Environmental Research and Consulting, The Dow Chemical Company, Midland, Michigan. Electronic address:

Developmental neurotoxicity (DNT) is an important endpoint for the safety assessment of chemicals. However, the current in vivo animal model for DNT assessment is resource-intensive and may not fully capture all mechanisms that may be relevant to DNT in humans. As a result, there is a growing need for more reliable, time- and cost-efficient approaches for DNT evaluation. Read More

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http://dx.doi.org/10.1016/j.neuro.2019.02.010DOI Listing
February 2019

High-resolution Regulatory Maps Connect Vascular Risk Variants to Disease Related Pathways.

Circ Genom Precis Med 2019 Feb 20. Epub 2019 Feb 20.

Science for Life Laboratory, KTH - Royal Institute of Technology, Solna, Sweden.

Background: Genetic variant landscape of coronary artery disease (CAD) is dominated by non-coding variants among which many occur within putative enhancers regulating the expression levels of relevant genes. It is crucial to assign the genetic variants to their correct genes both to gain insights into perturbed functions and better assess the risk of disease.

Methods: In this study, we generated high-resolution genomic interaction maps (~750 bases) in aortic endothelial, smooth muscle cells and THP-1 macrophages stimulated with lipopolysaccharide using Hi-C coupled with sequence capture targeting 25,429 features including variants associated with CAD. Read More

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http://dx.doi.org/10.1161/CIRCGEN.118.002353DOI Listing
February 2019

Molecular Mechanisms of Plant Regeneration.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan; email: , , , , , ,

Plants reprogram somatic cells following injury and regenerate new tissues and organs. Upon perception of inductive cues, somatic cells often dedifferentiate, proliferate, and acquire new fates to repair damaged tissues or develop new organs from wound sites. Wound stress activates transcriptional cascades to promote cell fate reprogramming and initiate new developmental programs. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-100434DOI Listing
February 2019

The Scope, Functions, and Dynamics of Posttranslational Protein Modifications.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

Systembiologie der Pflanze, Universität Hohenheim, 70599 Stuttgart, Germany; email:

Assessing posttranslational modification (PTM) patterns within protein molecules and reading their functional implications present grand challenges for plant biology. We combine four perspectives on PTMs and their roles by considering five classes of PTMs as examples of the broader context of PTMs. These include modifications of the N terminus, glycosylation, phosphorylation, oxidation, and N-terminal and protein modifiers linked to protein degradation. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-100211DOI Listing
February 2019

Mammalian Respiratory Complex I Through the Lens of Cryo-EM.

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge CB2 0XY, United Kingdom; email: , , ,

Single-particle electron cryomicroscopy (cryo-EM) has led to a revolution in structural work on mammalian respiratory complex I. Complex I (mitochondrial NADH:ubiquinone oxidoreductase), a membrane-bound redox-driven proton pump, is one of the largest and most complicated enzymes in the mammalian cell. Rapid progress, following the first 5-Å resolution data on bovine complex I in 2014, has led to a model for mouse complex I at 3. Read More

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http://dx.doi.org/10.1146/annurev-biophys-052118-115704DOI Listing
February 2019

Membrane Electroporation and Electropermeabilization: Mechanisms and Models.

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Faculty of Electrical Engineering, University of Ljubljana , SI-1000 Ljubljana, Slovenia; email: ,

Exposure of biological cells to high-voltage, short-duration electric pulses causes a transient increase in their plasma membrane permeability, allowing transmembrane transport of otherwise impermeant molecules. In recent years, large steps were made in the understanding of underlying events. Formation of aqueous pores in the lipid bilayer is now a widely recognized mechanism, but evidence is growing that changes to individual membrane lipids and proteins also contribute, substantiating the need for terminological distinction between electroporation and electropermeabilization. Read More

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http://dx.doi.org/10.1146/annurev-biophys-052118-115451DOI Listing
February 2019

How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits?

Authors:
Robert M Glaeser

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA; email:

Impressive though the achievements of single-particle cryo-electron microscopy are today, a substantial gap still remains between what is currently accomplished and what is theoretically possible. As is reviewed here, twofold or more improvements are possible as regards ( a) the detective quantum efficiency of cameras at high resolution, ( b) converting phase modulations to intensity modulations in the image, and ( c) recovering the full amount of high-resolution signal in the presence of beam-induced motion of the specimen. In addition, potential for improvement is reviewed for other topics such as optimal choice of electron energy, use of aberration correctors, and quantum metrology. Read More

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http://dx.doi.org/10.1146/annurev-biophys-070317-032828DOI Listing
February 2019

Multipotent Mesenchymal Stromal Cells and Lung Disease: Not Ready for Prime Time.

Ann Am Thorac Soc 2019 Feb 20. Epub 2019 Feb 20.

University of Miami - Miller School of Medicine, Pulmonary and Critical Care Medicine, Miami, Florida, United States.

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http://dx.doi.org/10.1513/AnnalsATS.201811-843PSDOI Listing
February 2019

Time-resolved quantification of the dynamic extracellular space in the brain during short-lived event: methodology and simulations.

J Neurophysiol 2019 Feb 20. Epub 2019 Feb 20.

Shantou University.

Two macroscopic parameters describe the interstitial diffusion of substances in the extracellular space (ECS) of the brain, the ECS volume fraction α and the diffusion tortuosity λ. Past methods based on sampling the extracellular concentration of a membrane-impermeable ion tracer, such as tetramethyl ammonium (TMA+), can characterize either the dynamic α(t) alone or the constant α and λ in resting state, but never the dynamic α(t) and λ(t) simultaneously in short-lived brain events. In this work, we propose to use a sinusoidal method of TMA+ to provide time-resolved quantification of α(t) and λ(t) in acute brain events. Read More

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http://dx.doi.org/10.1152/jn.00347.2018DOI Listing
February 2019

Maspin binds to cardiolipin in mitochondria and triggers apoptosis.

FASEB J 2019 Feb 20:fj201802182R. Epub 2019 Feb 20.

Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.

A central question in cell biology is how cells respond to stress signals and biochemically regulate apoptosis. One critical pathway involves the change of mitochondrial function and release of cytochrome c to initiate apoptosis. In response to apoptotic stimuli, we found that maspin-a noninhibitory member of the serine protease inhibitor superfamily-translocates from the cytosol to mitochondria and binds to cardiolipin in the inner mitochondrial membrane. Read More

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http://dx.doi.org/10.1096/fj.201802182RDOI Listing
February 2019

Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.

Annu Rev Anal Chem (Palo Alto Calif) 2019 Feb 20. Epub 2019 Feb 20.

Center for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, and Department of Chemistry, University of Virginia, Charlottesville, Virginia 22908, USA; email:

Although fluorescent reporters and biosensors have become indispensable tools in biological and biomedical fields, fluorescence measurements require external excitation light, thereby limiting their use in thick tissues and live animals. Bioluminescent reporters and biosensors may potentially overcome this hurdle because they use enzyme-catalyzed exothermic biochemical reactions to generate excited-state emitters. This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors. Read More

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http://dx.doi.org/10.1146/annurev-anchem-061318-115027DOI Listing
February 2019

Local Administration of Magnesium Promotes Meniscal Healing Through Homing of Endogenous Stem Cells: A Proof-of-Concept Study.

Am J Sports Med 2019 Feb 20:363546518820076. Epub 2019 Feb 20.

Department of Orthopaedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Background:: Although many strategies have been developed to modify the biological and biomechanical environment of the meniscal suture repair to improve the chances of healing, the failure rates remain high. Thus, new methods to promote meniscal regeneration and repair are needed.

Hypothesis:: Administration of magnesium (via a repair using magnesium stitches) might enhance recruitment and adherence of endogenous stem cells to the site of the lesion, thereby promoting in situ meniscal regeneration and chondroprotective functions. Read More

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http://dx.doi.org/10.1177/0363546518820076DOI Listing
February 2019

High Throughput Complementary Analysis and Quantitation of Metabolites by MALDI- and Silicon Nanopost Array-Laser Desorption Ionization Mass Spectrometry.

Anal Chem 2019 Feb 20. Epub 2019 Feb 20.

Silicon nanopost arrays (NAPA) have been shown to be effective substrates for laser desorption ionization-mass spectrometry (LDI-MS), and have been used to analyze a variety of samples including peptides, metabolites, drugs, explosives, and intact cells, as well as to image lipids and metabolites in tissue sections. However, no direct comparison has yet been conducted between NAPA-MS and the most commonly-used LDI-MS technique, matrix-assisted laser desorption ionization (MALDI)-MS. In this work, we compare the utility of NAPA-MS to that of MALDI-MS using two common matrices for the analysis of metabolites in cellular extracts and human urine. Read More

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http://dx.doi.org/10.1021/acs.analchem.8b05074DOI Listing
February 2019

DPD-inspired discovery of novel LsrK kinase inhibitors: an opportunity to fight antimicrobial resistance.

J Med Chem 2019 Feb 20. Epub 2019 Feb 20.

Antibiotic resistance is posing a continuous threat to global public health and represents a huge burden for society as a whole. In the last decade, the interference with bacterial Quorum Sensing (QS) (i.e. Read More

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http://dx.doi.org/10.1021/acs.jmedchem.9b00025DOI Listing
February 2019

Sacituzumab Govitecan-hziy in Refractory Metastatic Triple-Negative Breast Cancer.

N Engl J Med 2019 02;380(8):741-751

From the Massachusetts General Hospital Cancer Center (A.B., S.J.I.) and Dana-Farber Cancer Institute (S.M.T.), Harvard Medical School, Boston; Vanderbilt-Ingram Cancer Center, Nashville (I.A.M., V.G.A.); Weill Cornell Medical College (L.T.V.) and New York-Presbyterian-Columbia University Irving Medical Center (K.K.), New York; University of Colorado Cancer Center, Aurora (J.R.D.); Texas Oncology, Baylor University Medical Center, US Oncology, Dallas (J.O.); Orlando Health University of Florida Health Cancer Center, Orlando (R.L.M., N.C.S.); Yale University School of Medicine, New Haven, CT (A.D.S.); University of California, San Francisco, Helen Diller Family Comprehensive Cancer Center, San Francisco (H.S.R.); Immunomedics, Morris Plains, NJ (D.M.G., R.I., S.W., R.M.S., W.A.W.); and AIS Consulting, Ann Arbor, MI (A.M.S.).

Background: Standard chemotherapy is associated with low response rates and short progression-free survival among patients with pretreated metastatic triple-negative breast cancer. Sacituzumab govitecan-hziy is an antibody-drug conjugate that combines a humanized monoclonal antibody, which targets the human trophoblast cell-surface antigen 2 (Trop-2), with SN-38, which is conjugated to the antibody by a cleavable linker. Sacituzumab govitecan-hziy enables delivery of high concentrations of SN-38 to tumors. Read More

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http://www.nejm.org/doi/10.1056/NEJMoa1814213
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http://dx.doi.org/10.1056/NEJMoa1814213DOI Listing
February 2019
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Retraction: Steering CAR T Cells into Solid Tumors. N Engl J Med 2019;380:289-91.

N Engl J Med 2019 Feb 20. Epub 2019 Feb 20.

University of Oxford, Oxford, United Kingdom.

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http://dx.doi.org/10.1056/NEJMc1902526DOI Listing
February 2019

Design of CK2β-mimicking peptides as tools to study the CK2α/CK2β interaction in cancer cells.

ChemMedChem 2019 Feb 20. Epub 2019 Feb 20.

University of Cologne, Department of Pharmacology, Gleueler Strasse 24, 50931, Cologne, GERMANY.

The ubiquitously expressed Ser/Thr kinase CK2 is a key regulator in a variety of key processes in normal and malignant cells. Due to its distinctive anti-apoptotic and tumor-driving properties, elevated levels of CK2 have frequently been found in tumors of different origin. In recent years, development of CK2 inhibitors has largely been focused on ATP-competitive compounds; however, targeting the CK2α/CK2β interface has emerged as a further concept that might avoid selectivity issues. Read More

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http://dx.doi.org/10.1002/cmdc.201800786DOI Listing
February 2019

Increase of germ cell nuclear factor expression in globozoospermic Gopc knockout mice.

Andrology 2019 Feb 20. Epub 2019 Feb 20.

Department of Cell Biology and Histology, School of Medicine and Nursing, University of the Basque Country (UPV/EHU), Leioa, Spain.

Background: Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) is a Golgi protein that plays a role in vesicular transport and intracellular protein trafficking and degradation. Mice deficient in GOPC protein have globozoospermia and are infertile. The germ cell nuclear factor (GCNF) is a member of the nuclear receptor superfamily which is expressed in male germ cells, from spermatocytes and spermatids, both in the nucleus and the acrosomal region. Read More

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http://dx.doi.org/10.1111/andr.12594DOI Listing
February 2019

Histopathological and hematological changes in recurrent nasal polyposis.

Int Forum Allergy Rhinol 2019 Feb 20. Epub 2019 Feb 20.

Otolaryngology Section, Department of Neuroscience (DNS), Padova University, Padova, Italy.

Background: There is a dearth of information regarding the histological and hematological differences between primary and recurrent chronic rhinosinusitis with nasal polyps (CRSwNP). The present study analyzed the histological changes in recurrent CRSwNP in terms of eosinophilic infiltrate, subepithelial edema, goblet cell hyperplasia, and basement membrane thickness. Blood levels of eosinophils and basophils were also measured prior to surgery on both primary and recurrent disease. Read More

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http://dx.doi.org/10.1002/alr.22314DOI Listing
February 2019

Cellular origin of microRNA-371a-3p in healthy males based on systematic urogenital tract tissue evaluation.

Andrology 2019 Feb 20. Epub 2019 Feb 20.

Pathology (LEPO), Erasmus MC Cancer Institute, University Medical Center, Rotterdam, The Netherlands.

Background: The microRNA-371a-3p (miR-371a-3p) has been reported to be an informative liquid biopsy (serum and plasma) molecular biomarker for both diagnosis and follow-up of patients with a malignant (testicular) germ cell tumor ((T)GCT). It is expressed in all histological cancer elements, with the exception of mature teratoma. However, normal testis, semen, and serum of males with a disrupted testicular integrity without a TGCT may contain miR-371a-3p levels above threshold, of which the cellular origin is unknown. Read More

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http://dx.doi.org/10.1111/andr.12595DOI Listing
February 2019

Alpha lipoic acid reverses the negative effect of LPS on mouse spermatozoa and developmental competence of resultant embryos in vitro.

Andrology 2019 Feb 20. Epub 2019 Feb 20.

Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Background: Reproductive toxicity of lipopolysaccharide (LPS) on spermatozoa is well established.

Objective: The aim of the present study was to show the potential benefits of alpha lipoic acid (ALA) as a strong antioxidant in alleviating the reproductive toxicity of LPS.

Materials And Methods: Sperm cells and cumulus-oocyte complexes (COCs) were collected from healthy NMRI mice (body weights ranged from 25 to 35 g, 100 females and 200 males). Read More

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http://dx.doi.org/10.1111/andr.12596DOI Listing
February 2019

Hierarchical Microchannel Architecture in Chitosan/Bioactive Glass Scaffolds via Electrophoretic Deposition Positive-Replica.

J Biomed Mater Res A 2019 Feb 20. Epub 2019 Feb 20.

Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Milano, Italy.

One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way. As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing. Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes. Read More

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http://dx.doi.org/10.1002/jbm.a.36660DOI Listing
February 2019

Influence of NKG2C gene deletion and CCR5Δ32 in Pre-eclampsia-Approaching the effect of innate immune gene variants in pregnancy.

Int J Immunogenet 2019 Feb 20. Epub 2019 Feb 20.

Laboratório de Imunobiologia e Imunogenética, Programa de Pós-Graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.

Pre-eclampsia (PE) is a hypertensive disorder that affects an important number of pregnant women worldwide. The exact causes of PE remain poorly understood. However, inflammation and deregulation of innate immune cells, such as natural killer (NK) cells, contribute to PE pathogenesis. Read More

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http://dx.doi.org/10.1111/iji.12416DOI Listing
February 2019

Silk Fluorescence Collimator for Ultrasensitive Humidity Sensing and Light-Harvesting in Semitransparent Dye-Sensitized Solar Cells.

Small 2019 Feb 20:e1804171. Epub 2019 Feb 20.

Research Institute for Biomimetics and Soft Matter, College of Materials, College of Physical Science and Technology, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Xiamen University, Xiamen, 361005, China.

This work examines the self-collimation effect of silk materials on fluorescence emission/detection. A macroscopic regulation strategy, coupled with meso-reconstruction and meso-functionalization, is adopted to amplify the fluorescence emission of organic fluorescent dyes (i.e. Read More

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http://dx.doi.org/10.1002/smll.201804171DOI Listing
February 2019

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices.

J Biomed Mater Res A 2019 Feb 20. Epub 2019 Feb 20.

Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.

Due to the similar collagen composition and closely physiological relationship with soft connective tissues, demineralized bone matrices (DBMs) were used to repair the injured tendon or ligament. However, the osteoinductivity of DBMs would be a huge barrier of these applications. Hydrogen peroxide (H O ) has been proved to reduce the osteoinductivity of DBMs. Read More

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http://dx.doi.org/10.1002/jbm.a.36662DOI Listing
February 2019

Roles of electrical stimulation in promoting osteogenic differentiation of BMSCs on conductive fibers.

J Biomed Mater Res A 2019 Feb 20. Epub 2019 Feb 20.

School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States.

The strategy of using conductive materials in regenerating bone defects is attractive benefiting from the bioelectricity feature of natural bone tissues. Thereby, POP conductive fibers were fabricated by coating polypyrrole (PPY) onto electrospun poly(L-lactide) (PLLA) fibers, and their potentials in promoting osteogenic differentiation of bone mesenchymal stromal cells (BMSCs) were investigated. Different from the smooth-surfaced PLLA fibers, POP fibers were rough-surfaced and favorable for protein adsorption and mineralization nucleation. Read More

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http://dx.doi.org/10.1002/jbm.a.36659DOI Listing
February 2019

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell.

Small 2019 Feb 20:e1805416. Epub 2019 Feb 20.

Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

Advances in DNA nanotechnology empower the programmable assembly of DNA building blocks (oligonucleotides and plasmids) into DNA nanostructures with precise architectural control. As DNA nanostructures are biocompatible and can naturally enter mammalian cells without the aid of transfection agents, they have found numerous biological or biomedical applications as delivery carriers of therapeutic and imaging cargoes into mammalian cells for at least a decade. Nevertheless, mechanistic studies on how DNA nanostructures interact with cells have remained limited and incomprehensive until 2-3 years ago. Read More

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http://dx.doi.org/10.1002/smll.201805416DOI Listing
February 2019

Age- and sex-specific reference intervals for hematologic analytes in Chinese children.

Int J Lab Hematol 2019 Feb 20. Epub 2019 Feb 20.

Department of Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Introduction: Reference intervals for pediatric laboratory tests need to be interpreted in the context of age- and sex-dependent dynamics. However, few reference intervals for healthy ethnic Han Chinese children have previously attempted to establish using large sample sizes. As such, there are no national hematological standards in China for pediatric reference intervals. Read More

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http://dx.doi.org/10.1111/ijlh.12979DOI Listing
February 2019

Impact of maternal body mass index and gestational weight gain on pregnancy complications: An individual participant data meta-analysis of European, North American and Australian cohorts.

BJOG 2019 Feb 20. Epub 2019 Feb 20.

The Generation R Study Group, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.

Objective: To assess the separate and combined associations of maternal pre-pregnancy BMI and gestational weight gain with the risks of pregnancy complications and their population impact.

Design: Individual participant data meta-analysis of 39 cohorts.

Setting: Europe, North America and Oceania. Read More

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http://dx.doi.org/10.1111/1471-0528.15661DOI Listing
February 2019

Evaluation of pulmonary toxicity of benzalkonium chloride and triethylene glycol mixtures using in vitro and in vivo systems.

Environ Toxicol 2019 Feb 20. Epub 2019 Feb 20.

Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research, Incheon, Republic of Korea.

Benzalkonium chloride (BAC) is a widely used disinfectant/preservative, and respiratory exposure to this compound has been reported to be highly toxic. Spray-form household products have been known to contain BAC together with triethylene glycol (TEG) in their solutions. The purpose of this study was to estimate the toxicity of BAC and TEG mixtures to pulmonary organs using in vitro and in vivo experiments. Read More

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http://dx.doi.org/10.1002/tox.22722DOI Listing
February 2019

Successful hematopoietic stem cell transplantation from an HLA-mismatched parent for engraftment failure after unrelated cord blood transplantation in patients with juvenile myelomonocytic leukemia: Report of two cases.

Pediatr Transplant 2019 Feb 20:e13378. Epub 2019 Feb 20.

Department of Pediatrics, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.

JMML is an aggressive hematopoietic malignancy of early childhood, and allogeneic HSCT is the only curative treatment for this disease. Umbilical cord blood is one of donor sources for HSCT in JMML patients who do not have an HLA-compatible relative, but engraftment failure remains a major problem. Here, we report two cases of JMML who were successfully rescued by HSCT from an HLA-mismatched parent after development of primary engraftment failure following unrelated CBT. Read More

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http://dx.doi.org/10.1111/petr.13378DOI Listing
February 2019

HspBP1 and anti-HspBP1 levels in the serum of HIV-infected individuals are associated to the disease progression.

J Appl Microbiol 2019 Feb 20. Epub 2019 Feb 20.

Laboratory of Cellular and Molecular Immunology - Federal University of Health Sciences of Porto Alegre, Av. Sarmento Leite, 245, Centro Historico, 90050-170, Porto Alegre, RS, Brazil.

Aims: The objective of this research was to quantify the levels of circulating HspBP1 and anti-HspBP1 IgG in HIV-infected individuals and to correlate them with CD4 T cell counts and viral load, as well as to determine the kinetics of those proteins during acute phase.

Methods And Results: Sixty serum samples from HIV-positive outpatients, thirty with high viral load and thirty with low viral load were analyzed. The HspBP1and anti-HspBP1 were quantified by ELISA. Read More

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http://dx.doi.org/10.1111/jam.14230DOI Listing
February 2019

Hematopoietic stem cell transplantation in children with sickle cell anemia: The parents' experience.

Pediatr Transplant 2019 Feb 20:e13376. Epub 2019 Feb 20.

Department of Psychopathology, Avicenne Hospital, Paris XIII University & Assistance Publique-Hôpitaux de Paris, Bobigny, France.

Genoidentical HSCT is currently the only curative treatment for SCA, preventing further vascular complications in high-risk children. Studies on the psychological implications of HSCT for recipient, sibling donor, and the rest of the family have been limited in SCA. This study enrolled ten families and used semi-structured interviews to explore the parents' experience at three time points: first before transplantation, then 3 months later, and 1 year later. Read More

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http://dx.doi.org/10.1111/petr.13376DOI Listing
February 2019

Creating Cell and Animal Models of Human Disease by Genome Editing Using CRISPR/Cas9.

J Gene Med 2019 Feb 20:e3082. Epub 2019 Feb 20.

Department of Genetics, Shiraz University of Medical Science, Shiraz, Iran.

Aims: Set of unique sequences in bacterial genomes, responsible for protecting bacteria against bacteriophages, have been recently used for genetic manipulation of specific points in the genome. These system comprises of one RNA component and one enzyme component, known as CRISPR and Cas9, respectively. The present review focuses on the applications of the CRISPR/Cas9 technology in the development of cellular and animal models of human disease. Read More

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http://dx.doi.org/10.1002/jgm.3082DOI Listing
February 2019
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