6,976,375 results match your criteria Cell & Chromosome [Journal]

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Surmounting the obstacles that impede effective CAR T cell trafficking to solid tumors.

J Leukoc Biol 2020 Jul 3. Epub 2020 Jul 3.

Laboratory on Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.

Innovative immunotherapies based on immune checkpoint targeting antibodies and engineered T cells are transforming the way we approach cancer treatment. However, although these T cell centered strategies result in marked and durable responses in patients across many different tumor types, they provide therapeutic efficacy only in a proportion of patients. A major challenge of immuno-oncology is thereby to identify mechanisms responsible for resistance to cancer immunotherapy in order to overcome them via adapted strategies that will ultimately improve intrinsic efficacy and response rates. Read More

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http://dx.doi.org/10.1002/JLB.1MR0520-746RDOI Listing

Interplay between alveolar epithelial and dendritic cells and Mycobacterium tuberculosis.

J Leukoc Biol 2020 Jul 3. Epub 2020 Jul 3.

Basic and Applied Immunology Program, Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of Sao Paulo, Sao Paulo, Brazil.

The innate response plays a crucial role in the protection against tuberculosis development. Moreover, the initial steps that drive the host-pathogen interaction following Mycobacterium tuberculosis infection are critical for the development of adaptive immune response. As alveolar Mϕs, airway epithelial cells, and dendritic cells can sense the presence of M. Read More

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http://dx.doi.org/10.1002/JLB.4MR0520-112RDOI Listing

Anti-CD20 rituximab IgG1, IgG3, and IgG4 but not IgG2 subclass trigger Ca mobilization and cytotoxicity in human NK cells.

J Leukoc Biol 2020 Jul 3. Epub 2020 Jul 3.

Division of Immunology and Allergology, Department of Medicine, University Hospitals and Medical Faculty, Geneva, Switzerland.

NK cell-mediated Ab-dependent cellular cytotoxicity (ADCC) is increasingly recognized to play an important role in cancer immunotherapy, transplant rejection, and autoimmunity. However, several aspects of the molecular interactions of IgG subclasses with the Fc-gamma receptor IIIA (FcγRIIIA)/CD16a expressed on NK cells remain unknown. The aim of the current study was to further analyze the role of IgG subclasses and FCGR3A V158F single nucleotide polymorphism (SNP) on Ca signaling and NK cell-mediated ADCC against Daudi target cells in vitro. Read More

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http://dx.doi.org/10.1002/JLB.5MA0620-039RDOI Listing

Identification of the association between HBcAg-specific T cell and viral control in chronic HBV infection using a cultured ELISPOT assay.

J Leukoc Biol 2020 Jul 3. Epub 2020 Jul 3.

State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Hepatitis B virus (HBV)-specific T cells play a critical role in determining the outcome of HBV infection. However, T cell response induced by predominant Ag in chronic infection is hardly detectable owing to the lack of a suitable assay. We herein established an optimized method to enumerate HBV-specific T cells and assessed the association between HBV surface Ag (HBsAg) and HBV DNA. Read More

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http://dx.doi.org/10.1002/JLB.5MA0620-023RRDOI Listing

Guanylate binding proteins contained in the murine chromosome 3 are important to control mycobacterial infection.

J Leukoc Biol 2020 Jul 3. Epub 2020 Jul 3.

Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Minas Gerais, Belo Horizonte, Brazil.

Guanylate binding proteins (GBPs) are important effector molecules of autonomous response induced by proinflammatory stimuli, mainly IFNs. The murine GBPs clustered in chromosome 3 (GBPchr3) contains the majority of human homologous GBPs. Despite intense efforts, mycobacterial-promoted diseases are still a major public health problem. Read More

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http://dx.doi.org/10.1002/JLB.4MA0620-526RRDOI Listing

Small molecule 3PO inhibits glycolysis but does not bind to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3).

FEBS Lett 2020 Jul 3. Epub 2020 Jul 3.

Laboratory of Physiopharmacology, University of Antwerp, Antwerp, Belgium.

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3) is a key enzyme of the glycolytic pathway, and it plays an essential role in angiogenesis. 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO) is frequently used as a glycolysis inhibitor and is thought to inhibit PFKFB3. However, this latter effect of 3PO has never been investigated in detail and was the aim of the present study. Read More

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http://dx.doi.org/10.1002/1873-3468.13878DOI Listing

Analysis of lncRNAs-miRNAs-mRNAs networks in periodontal ligament stem cells under mechanical force.

Oral Dis 2020 Jul 3. Epub 2020 Jul 3.

Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, China.

Objectives: Our study aims to analyze the expression profiles of long non-coding RNAs (lncRNAs) and investigate the potential regulatory networks among lncRNAs, microRNAs (miRNAs) and mRNAs in periodontal ligament stem cells (PDLSCs) under mechanical force (MF).

Materials And Methods: PDLSCs were isolated from human periodontal ligament tissues and identified by flow cytometry analysis. Multidirectional differentiation potential of PDLSCs was obtained by osteogenic and adipogenic induction. Read More

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http://dx.doi.org/10.1111/odi.13530DOI Listing

Efficient T cell compartment in HIV+ patients receiving orthotopic liver transplant and immunosuppressive therapy.

J Infect Dis 2020 Jul 4. Epub 2020 Jul 4.

Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, Modena, Italy.

Background And Aims: In patients undergoing orthotopic liver transplant (OLT), immunosuppressive (IS) treatment is mandatory and infections are leading causes of morbidity/mortality. Thus, it is essential to understand the functionality of cell-mediated immunity after OLT. The aim of the study was to identify changes in T cell phenotype and polyfunctionality in HIV+ and HIV- patients treated with IS after OLT. Read More

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http://dx.doi.org/10.1093/infdis/jiaa395DOI Listing

Impaired Th1 response is associated therapeutic failure in patients with cutaneous leishmaniasis caused by Leishmania braziliensis.

J Infect Dis 2020 Jul 3. Epub 2020 Jul 3.

Instituto Gonçalo Moniz, FIOCRUZ, Salvador, Bahia, Brazil.

Background: Leishmania skin test (LST) evaluates the delayed type hypersensitivity to Leishmania antigens (LA) and has been used for diagnosis of cutaneous leishmaniasis (CL). CL patients usually present a positive LST, but as small percentage have a negative LST (LST-). The aim of this study was to determine the clinic and immunologic features and response to antimony therapy in LST- CL patients. Read More

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http://dx.doi.org/10.1093/infdis/jiaa374DOI Listing

The relationship between CD4+ follicular helper T cells and CD8+ resident memory T cells: sisters or distant cousins?

Int Immunol 2020 Jul 3. Epub 2020 Jul 3.

University of Minnesota Medical School, Center for Immunology, Department of Laboratory Medicine and Pathology, Minneapolis, MN, USA.

Independent studies over the last decade have characterized the properties of non-circulating CD8+ "resident" memory T cells (TRM), which offer barrier protective immunity in non-lymphoid tissues and CD4+ follicular helper T cells (TFH), which mediate B cell help in lymphoid sites. Despite their very different biological roles in the immune system, intriguing parallels have been noted between the trafficking properties and differentiation cues of these populations, parallels which have only sharpened with recent findings. In this review, we explore the features that underlie these similarities and discuss whether these indicate meaningful homologies in the development of CD8+ TRM and CD4+ TFH or reflect resemblances which are only "skin-deep". Read More

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http://dx.doi.org/10.1093/intimm/dxaa045DOI Listing

Realization of a Push-Me-Pull-You swimmer at low Reynolds numbers.

Bioinspir Biomim 2020 Jul 3. Epub 2020 Jul 3.

Dept. of Bioengineering, Santa Clara University, Santa Clara, California, UNITED STATES.

Locomotion at low Reynolds numbers encounters stringent physical constraints due to the dominance of viscous over inertial forces. A variety of swimming microorganisms has demonstrated diverse strategies to generate self-propulsion in the absence of inertia. In particular, ameboid and euglenoid movements exploit shape deformations of the cell body for locomotion. Read More

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http://dx.doi.org/10.1088/1748-3190/aba2b9DOI Listing

Bioprinting small diameter blood vessel constructs with an endothelial and smooth muscle cell bilayer in a single step.

Biofabrication 2020 Jul 3. Epub 2020 Jul 3.

Wake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina, UNITED STATES.

Bioengineered artificial blood vessels have been a major area of interest over the last decade. Of particular interest are small diameter vessels, as surgical options are currently limited. This study aimed to fabricate a small diameter, heterogeneous bilayer blood vessel-like construct in a single step with gelatin methacryloyl (GelMA) bioink using a 3D micro-extrusion bioprinter on a solid platform. Read More

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http://dx.doi.org/10.1088/1758-5090/aba2b6DOI Listing

Calcium phosphate nanoparticles as intrinsic inorganic antimicrobials: mechanism of action.

Biomed Mater 2020 Jul 3. Epub 2020 Jul 3.

Division of Biomaterials and Biomedical Engineering, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA, San Francisco, UNITED STATES.

This is the final report of the study aimed at assessing the antimicrobial activity of CP nanoparticles delivered in the form of hydroxyapatite (HAp) or amorphous CP (ACP) and understanding the fundamental principles behind their mechanisms of action. Not responding to propidium iodide and causing no gross morphological changes except moderate stress-induced filamentation in E. coli, CP nanoparticles were shown to be bacteriostatic, not bactericidal. Read More

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http://dx.doi.org/10.1088/1748-605X/aba281DOI Listing

Redispersion of cryoaggregated gold nanoparticle by means of laser irradiation and effect on biological interactions.

Nanotechnology 2020 Jul 3. Epub 2020 Jul 3.

Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, INDIA.

Agglomeration/aggregation is an indispensable phenomenon observed by different nanoparticles. In the present study, commercial grade (50nm) and chemically synthesized (40nm) gold nanoparticles (AuNPs) were aggregated at sub-zero temperatures, followed by disruption of the AuNP aggregates via nanosecond laser-ablation and subsequent effect on biological interactions. AuNPs were characterized pre/post laser-ablation via UV-visible spectroscopy, transmission electron microscopy, atomic force microscopy, etc. Read More

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http://dx.doi.org/10.1088/1361-6528/aba2a1DOI Listing

Blood glutamate scavengers and exercises as an effective neuroprotective treatment in mice with spinal cord injury.

J Neurosurg Spine 2020 Jul 3:1-13. Epub 2020 Jul 3.

1Steyer School of Health Professions, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

Objective: Excitotoxicity due to neuronal damage and glutamate release is one of the first events that leads to the progression of neuronal degeneration and functional impairment. This study is based on a paradigm shift in the therapeutic approach for treating spinal cord injury (SCI). The authors tested a new treatment targeting removal of CNS glutamate into the blood circulation by injection of the blood glutamate scavengers (BGSs) recombinant enzyme glutamate-oxaloacetate transaminase (rGOT1) and its cosubstrate oxaloacetic acid (OxAc). Read More

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http://dx.doi.org/10.3171/2020.4.SPINE20302DOI Listing

Mechanical control of plant morphogenesis: concepts and progress.

Authors:
Fei Du Yuling Jiao

Curr Opin Plant Biol 2020 Jun 30;57:16-23. Epub 2020 Jun 30.

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Understanding how the genome encodes organismal shape is fundamental to biology. Extensive molecular genetic studies have uncovered genes regulating morphogenesis, that is, the generation of shape, however, such genes do not directly determine cell and tissue shape. Recent studies have started to elucidate how mechanical cues mediate the physical shaping of cells and tissues. Read More

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http://dx.doi.org/10.1016/j.pbi.2020.05.008DOI Listing

Broadly neutralizing antibodies potently inhibit cell-to-cell transmission of semen leukocyte-derived SHIV162P3.

EBioMedicine 2020 Jun 30;57:102842. Epub 2020 Jun 30.

Université Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial diseases ≫ (IMVA-HB/IDMIT), Fontenay-aux-Roses & Le Kremlin-Bicêtre, France. Electronic address:

Background: HIV-1 sexual transmission occurs mostly through infected semen, which contains both free virions and infected leukocytes. Transmission initiated by infected cells has been shown by several in vitro and in vivo studies and a reduced capacity of broadly neutralizing antibodies (bNAbs) to inhibit cell-to-cell transmission has also been reported. However, due to limitations of available experimental models, there is yet no clarity to which extend bNAbs can prevent transmission mediated by semen leukocytes. Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102842DOI Listing

Development of an immune gene prognostic classifier for survival prediction and respond to immunocheckpoint inhibitor therapy/chemotherapy in endometrial cancer.

Int Immunopharmacol 2020 Jun 30;86:106735. Epub 2020 Jun 30.

Department of Gynecology, the First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China. Electronic address:

Immunotherapy has provided a promising therapeutic strategy for endometrial cancer (EC). The present study aims to develop a prognostic classifier based on immune-related genes (IRGs) to stratify EC patients. A total of 15 prognosis-related IRGs were further filtrated by multivariate Cox regression: LTA, TMSB15A, S100A14, PLA2G2A, PDGFRA, CLDN4, CTF1, PRLH, PTN, SST, HTR3E, NRP1, RORA, THRA and CBLC. Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106735DOI Listing

Nitric oxide alleviates lignification and softening of water bamboo (Zizania latifolia) shoots during postharvest storage.

Food Chem 2020 Jun 27;332:127416. Epub 2020 Jun 27.

Department of Food Science, School of Food Science and Engineering, Yangzhou University, Yangzhou, China. Electronic address:

Water bamboo shoots quickly deteriorate after harvest as a result of rapid lignification and softening. Nitric oxide (NO) has been used to extend the postharvest life of several other vegetables. Here, we examined the effect of NO on the storage of water bamboo shoots at 4℃ for 28 days. Read More

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http://dx.doi.org/10.1016/j.foodchem.2020.127416DOI Listing

MiR-483-3p inhibition ameliorates myocardial ischemia/reperfusion injury by targeting the MDM4/p53 pathway.

Mol Immunol 2020 Jun 30;125:9-14. Epub 2020 Jun 30.

Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, PR China. Electronic address:

MiR-483-3p is involved in the pathogenesis of acute myocardial infarctions, but its association with myocardial ischemia reperfusion (IR) remains mostly unknown. In this study, an in vitro model of myocardial IR injury was established by putting H9c2 cells into hypoxia reoxygenation (HR) treatment to explore the effects and possible mechanisms of miR-483-3p in myocardial IR injury. HR exposure resulted in increased miR-483-3p levels in H9c2 cells. Read More

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http://dx.doi.org/10.1016/j.molimm.2020.06.014DOI Listing

Inhibition of PIM1 kinase attenuates bleomycin-induced pulmonary fibrosis in mice by modulating the ZEB1/E-cadherin pathway in alveolar epithelial cells.

Mol Immunol 2020 Jun 30;125:15-22. Epub 2020 Jun 30.

Department of Anesthesiology, Weifang Medical University, Weifang, China; Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

PIM1 is serine/threonine protein kinase that is involved in numerous biological processes. Pulmonary fibrosis (PF) is a chronic pathological result of the dysfunctional repair of lung injury without effective therapeutic treatments. In the current study, we investigated whether PIM1 inhibition would improve bleomycin (BLM)-induced pulmonary fibrosis. Read More

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http://dx.doi.org/10.1016/j.molimm.2020.06.013DOI Listing

Radiomic analysis identifies tumor subtypes associated with distinct molecular and microenvironmental factors in head and neck squamous cell carcinoma.

Oral Oncol 2020 Jun 30;110:104877. Epub 2020 Jun 30.

Department of Medical Physics, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Purpose: To identify whether radiomic features from pre-treatment computed tomography (CT) scans can predict molecular differences between head and neck squamous cell carcinoma (HNSCC) using The Cancer Imaging Archive (TCIA) and The Cancer Genome Atlas (TCGA).

Methods: 77 patients from the TCIA with HNSCC had imaging suitable for analysis. Radiomic features were extracted and unsupervised consensus clustering was performed to identify subtypes. Read More

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http://dx.doi.org/10.1016/j.oraloncology.2020.104877DOI Listing

Clinicopathological features of 50 mismatch repair (MMR)-deficient endometrial carcinomas, tested by immunohistochemistry: A single institutional feasibility study, India.

Ann Diagn Pathol 2020 Jun 20;47:151558. Epub 2020 Jun 20.

Department of Radiation Oncology (Gynecology, Disease Management group), Tata Memorial Hospital, HBNI University, Mumbai, India.

There are few comprehensive studies from Asia on clinicopathologic features of mismatch repair (MMR)-deficient endometrial carcinomas, including rarely from our country. One hundred and four cases of endometrial carcinomas were tested for four MMR proteins by immunohistochemistry. Among 50 MMR-deficient (MMRd) tumors(48%), age-range was 27-68 years(median = 53) and tumor size(n = 34) varied from 1. Read More

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http://dx.doi.org/10.1016/j.anndiagpath.2020.151558DOI Listing

Arsenic exposure-related hyperglycemia is linked to insulin resistance with concomitant reduction of skeletal muscle mass.

Environ Int 2020 Jun 30;143:105890. Epub 2020 Jun 30.

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. Electronic address:

Background: Alargebodyof evidence has shown a link between arsenic exposure and diabetes, but the underlying mechanisms have not yet been clarified.

Objective: We explored the association between arsenic exposure and the reduction of skeletal muscle mass as a potential mechanism of insulin resistance for developing arsenic-related hyperglycemia.

Methods: A total of 581 subjects were recruited from arsenic-endemic and non-endemic areas in Bangladesh and their fasting blood glucose (FBG), serum insulin, and serum creatinine levels were determined. Read More

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http://dx.doi.org/10.1016/j.envint.2020.105890DOI Listing

Polymorphisms of HLA-B: influences on assembly and immunity.

Curr Opin Immunol 2020 Jun 30;64:137-145. Epub 2020 Jun 30.

Department of Microbiology and Immunology, Michigan Medicine, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address:

The major histocompatibility class I (MHC-I) complex functions in innate and adaptive immunity, mediating surveillance of the subcellular environment. In humans, MHC-I heavy chains are encoded by three genes: the human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. These genes are highly polymorphic, which results in the expression, typically, of six different HLA class I (HLA-I) proteins on the cell surface, and the presentation of diverse peptide antigens to CD8 T cells for broad surveillance against many pathogenic conditions. Read More

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http://dx.doi.org/10.1016/j.coi.2020.05.008DOI Listing

A divergent mode of activation of a nitrosyl iron complex with unusual antiangiogenic activity.

J Inorg Biochem 2020 Jun 20;210:111133. Epub 2020 Jun 20.

Departamento de Química Orgânica e Inorgânica, Grupo de Bioinorgânica, Universidade Federal do Ceará-UFC, P.O Box 6021, Fortaleza, CE CEP 60440-900, Brazil. Electronic address:

Nitric oxide (NO) and nitroxyl (HNO) have gained broad attention due to their roles in several physiological and pathophysiological processes. Remarkably, these sibling species can exhibit opposing effects including the promotion of angiogenic activity by NO compared to HNO, which blocks neovascularization. While many NO donors have been developed over the years, interest in HNO has led to the recent emergence of new donors. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2020.111133DOI Listing

Drug repurposing: Discovery of troxipide analogs as potent antitumor agents.

Eur J Med Chem 2020 Jun 26;202:112471. Epub 2020 Jun 26.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China. Electronic address:

Drug repurposing plays a vital role in the discovery of undescribed bioactivities in clinical drugs. Based on drug repurposing strategy, we for the first time reported a novel series of troxipide analogs and then evaluated their antiproliferative activity against MCF-7, PC3, MGC-803, and PC9 cancer cell lines and WPMY-1, most of which showed obvious selectivity toward PC-3 over the other three cancer cell lines and WPMY-1. Compound 5q, especially, could effectively inhibit PC3 with an IC value of 0. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112471DOI Listing

Design, synthesis and biological evaluation of 4-aniline quinazoline derivatives conjugated with hydrogen sulfide (HS) donors as potent EGFR inhibitors against L858R resistance mutation.

Eur J Med Chem 2020 Jun 27;202:112522. Epub 2020 Jun 27.

College of Pharmacy, Xuzhou Medical University, Xuzhou, 221004, PR China; Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221004, PR China.

In this study, a series of 4-aniline quinazoline derivatives bearing hydrogen sulfide (HS) donors were designed, synthesized and evaluated for biological activities. The synthesized compounds were screened for the enzymatic activities against EGFR and EGFR mutants by kinase target-based cell screening method. The results demonstrate that most compounds exhibit selectively inhibitory activities against TEL-EGFR-L858R-BaF3, especially compound 9h with GI = 0. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112522DOI Listing

Identification of dibucaine derivatives as novel potent enterovirus 2C helicase inhibitors: In vitro, in vivo, and combination therapy study.

Eur J Med Chem 2020 Jun 25;202:112310. Epub 2020 Jun 25.

Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Frontier Science Center for Immunology and Metabolism, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China. Electronic address:

Enterovirus A71 (EV-A71) is a human pathogen causing hand, foot and mouth disease (HFMD) which seriously threatened the safety and lives of infants and young children. However, there are no licensed direct antiviral agents to cure the HFMD. In this study, a series of quinoline formamide analogues as effective enterovirus inhibitors were developed, subsequent systematic structure-activity relationship (SAR) studies demonstrated that these quinoline formamide analogues exhibited good potency to treat EV-A71 infection. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112310DOI Listing

Genome-wide non-invasive prenatal testing in single- and multiple-pregnancies at any risk: Identification of maternal polymorphisms to reduce the number of unnecessary invasive confirmation testing.

Eur J Obstet Gynecol Reprod Biol 2020 Jun 2;252:19-29. Epub 2020 Jun 2.

Institute of Medical Genetics, University of Zurich, Zurich, Switzerland.

Objective: Non-invasive prenatal testing by targeted or genome-wide copy number profiling (cnNIPT) has the potential to outperform standard NIPT targeting the common trisomies 13, 18, and 21, only. Nevertheless, prospective results and outcome data on cnNIPT are still scarce and there is increasing evidence for maternal copy number variants (CNVs) interfering with results of both, standard and cnNIPT.

Study Design: We assessed the performance of cnNIPT in 3053 prospective and 116 retrospective cases with special consideration of maternal CNVs in singleton and multiple gestational pregnancies at any risk, as well as comprehensive follow-up. Read More

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http://dx.doi.org/10.1016/j.ejogrb.2020.05.070DOI Listing

Artificial protein cages - inspiration, construction, and observation.

Curr Opin Struct Biol 2020 Jun 30;64:66-73. Epub 2020 Jun 30.

Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-392 Krakow, Poland. Electronic address:

Protein cages are hollow, often spherical, protein structures. They are scientifically interesting for reasons including their capability to serve as protective containers for delivering medically useful cargoes to cells. Design and construction of artificial protein cages is a powerful strategy enabling them to be endowed with bespoke properties not seen in natural forms. Read More

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http://dx.doi.org/10.1016/j.sbi.2020.05.014DOI Listing

Disruption of oligodendrocyte progenitor cells is an early sign of pathology in the triple transgenic mouse model of Alzheimer's disease.

Neurobiol Aging 2020 Jun 7;94:130-139. Epub 2020 Jun 7.

Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK. Electronic address:

There is increasing evidence that myelin disruption is related to cognitive decline in Alzheimer's disease (AD). In the CNS, myelin is produced by oligodendrocytes, which are generated throughout life by adult oligodendrocyte progenitor cells (OPCs), also known as NG2-glia. To address whether alterations in myelination are related to age-dependent changes in OPCs, we analyzed NG2 and myelin basic protein (MBP) immunolabelling in the hippocampus of 3×Tg-AD mice at 6 and 24 months of age, compared with non-Tg age-matched controls. Read More

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http://dx.doi.org/10.1016/j.neurobiolaging.2020.05.016DOI Listing

The feasibility and usability of DNA-dot bioconjugation to antibody for targeted in vitro cancer cell fluorescence imaging.

J Photochem Photobiol B 2020 Jun 26;209:111944. Epub 2020 Jun 26.

Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

DNA-protein bioconjugation is an appealing target-tracking strategy. The new capability of DNA molecule as a biological nanomaterial endows unique fluorescence and physicochemical properties to be applied in bioimaging. Progression in targeted imaging is contingent on the conjugation of diagnostic nanoparticles to biomolecular signatures, particularly antibody-based ligands. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2020.111944DOI Listing

Preoperative Type and Screen is Unnecessary in Elective Anatomic Lung Resection and Esophagectomy.

J Surg Res 2020 Jun 30;255:411-419. Epub 2020 Jun 30.

Geisel School of Medicine, Hanover, New Hampshire; Department of Surgery, Section of Thoracic Surgery, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire. Electronic address:

Background: Preoperative type and screen (TS) is routinely performed before elective thoracic surgery. We sought to evaluate the utility of this practice by examining our institutional data related to intraoperative and postoperative transfusions for two common, complex procedures.

Materials And Methods: A single-center, retrospective review of a prospective thoracic surgery database was performed. Read More

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http://dx.doi.org/10.1016/j.jss.2020.05.087DOI Listing

Dietary oligosaccharides attenuate DSS-induced colitis in mice, induce PGlyRP3 expression, and inhibit NF-κB and MEK/ERK signaling.

Authors:
Ayman Hyder

Cell Immunol 2020 Jun 10;354:104144. Epub 2020 Jun 10.

Faculty of Science, Damietta University, New Damietta 34517, Egypt. Electronic address:

Peptidoglycan recognition protein 3 (PGlyRP3) is a pattern recognition protein found in the gut epithelia and proven to have an anti-inflammatory effect in response to dietary lipids via the upregulation of PPARγ. We have reported an in vitro anti-inflammatory action for prebiotic oligosaccharides in the intestinal Caco-2 cells through stimulation of PPARγ and PGlyRP3. In the present study, this effect was examined in vivo in a DSS-induced colitis mouse model. Read More

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http://dx.doi.org/10.1016/j.cellimm.2020.104144DOI Listing

Definitive chemoradiotherapy plus cetuximab for cancer in the oesophagus or gastro-oesophageal junction.

Cancer Treat Res Commun 2020 Jun 27;24:100187. Epub 2020 Jun 27.

Department of Oncology, Rigshospitalet, Copenhagen, Denmark.

Background: Chemoradiotherapy is standard treatment for localized oesophageal cancer unsuitable for surgery. We aimed to evaluate the efficacy of cetuximab in combination with chemoradiotherapy.

Methods: This non-randomised multicentre phase II trial recruited patients aged 18-75 with WHO performance status 0-2 having squamous cell carcinoma or adenocarcinoma in the oesophagus or gastro-oesophageal junction, T2-4, N0-3, M0 not suitable for surgery. Read More

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http://dx.doi.org/10.1016/j.ctarc.2020.100187DOI Listing

Update on Müller glia regenerative potential for retinal repair.

Curr Opin Genet Dev 2020 Jun 30;64:52-59. Epub 2020 Jun 30.

Université Paris-Saclay, CNRS, Retina France, Institut des Neurosciences Paris Saclay, Orsay, France. Electronic address:

Retinal regeneration efficiency from Müller glia varies tremendously among vertebrate species, being extremely limited in mammals. Efforts towards the identification of molecular mechanisms underlying Müller cell proliferative and neurogenic potential should help finding strategies to awake them and ensure regeneration in mammals. We provide here an update on the most recent and original progresses made in the field. Read More

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http://dx.doi.org/10.1016/j.gde.2020.05.025DOI Listing

The advantages of using cytospins of uterine lavage fluid for the diagnosis of equine endometritis.

Theriogenology 2020 May 21;154:143-151. Epub 2020 May 21.

Animal Reproduction Center of Vairão, ICBAS-UP, Portugal.

Uterine lavage (UL) is a routine diagnostic procedure for endometritis. In UL the fluid is centrifuged and the sediment smeared. Samples prepared in cytocentrifuges, the so-called "cytospins", are useful for evaluating cells in fluids, but never been used in UL. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2020.05.008DOI Listing

The effect of the ghrelin-receptor agonist capromorelin on glucose metabolism in healthy cats.

Domest Anim Endocrinol 2020 Apr 18;74:106484. Epub 2020 Apr 18.

Department of Veterinary Medicine and Epidemiology, College of Veterinary Medicine, University of California, Davis, 1 Shields Ave, Davis, CA 95616, USA; Department of Small Animal Clinical Sciences, University of Florida, College of Veterinary Medicine, 2015 SW 16th Ave, Gainesville, FL 32610, USA. Electronic address:

Somatostatin secretion from islet delta cells is important in maintaining low glycemic variability (GV) by providing negative feedback to beta cells and inhibiting insulin secretion. Capromorelin is a ghrelin-receptor agonist that activates the growth hormone secretagogue receptor on delta cells. We hypothesized that in cats, capromorelin administration will result in decreased GV at the expense of reduced insulin secretion and glucose tolerance. Read More

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http://dx.doi.org/10.1016/j.domaniend.2020.106484DOI Listing

IL-7 receptor alpha defines heterogeneity and signature of human effector memory CD8 T cells in high dimensional analysis.

Cell Immunol 2020 Jun 24;355:104155. Epub 2020 Jun 24.

Departments of Internal Medicine and Yale University School of Medicine, New Haven, CT 06520, USA. Electronic address:

The IL-7 receptor alpha chain (IL-7Rα or CD127) can be differentially expressed in memory CD8 T cells. Here we investigated whether IL-7Rα could serve as a key molecule in defining a comprehensive landscape of heterogeneity in human effector memory (EM) CD8 T cells using high-dimensional Cytometry by Time-Of-Flight (CyTOF) and single-cell RNA-seq (scRNA-seq). IL-7Rα had diverse, but organized, expressional relationship in EM CD8 T cells with molecules related to cell function and gene regulation, which rendered an immune landscape defining heterogeneous cell subsets. Read More

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http://dx.doi.org/10.1016/j.cellimm.2020.104155DOI Listing

Introduction to this Cellular Immunology issue on new advances in solid organ transplantation immunology.

Cell Immunol 2020 Jun 27;355:104156. Epub 2020 Jun 27.

Department of Medicine, University of Chicago, Chicago, IL, United States. Electronic address:

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http://dx.doi.org/10.1016/j.cellimm.2020.104156DOI Listing

Combined TLR4 and TLR9 agonists induce distinct phenotypic changes in innate immunity in vitro and in vivo.

Cell Immunol 2020 Jun 14;355:104149. Epub 2020 Jun 14.

School of Biological Sciences, University of Nebraska-Lincoln, NE 68583, USA; Nebraska Center for Virology, University of Nebraska-Lincoln, NE 68583, USA. Electronic address:

Toll-like receptor (TLR)4 and TLR9 agonists, MPL and CpG, are used as adjuvants in vaccines and have been investigated for their combined potential. However, how these two combined agonists regulate transcriptional changes in innate immune cells and cells at the site of vaccination has not been thoroughly investigated. Here, we utilized transcriptomics to investigate how CpG, MPL, and CpG + MPL impact gene expression in dendritic cells (DC) in vitro. Read More

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http://dx.doi.org/10.1016/j.cellimm.2020.104149DOI Listing

Optimizing 5-aza-2'-deoxycytidine treatment to enhance the development of porcine cloned embryos by inhibiting apoptosis and improving DNA methylation reprogramming.

Res Vet Sci 2020 Jun 25;132:229-236. Epub 2020 Jun 25.

College of Veterinary Medicine, Qingdao Agricultural University, Shandong Province, Qingdao, China. Electronic address:

Apoptosis and incomplete DNA methylation reprogramming in cloned embryos reduce cloning efficiency. 5-aza-2'-deoxycytidine (5-aza-dC) is proven to regulate apoptosis and DNA methylation reprogramming, however, the treatment method and potential role of 5-aza-dC during cloned embryo development are still not well studied. This study displayed that treating donor cells with 5-aza-dC (AN group) significantly reduced the blastocyst rate, while treating cloned embryos (NA group) or both donor cells and cloned embryos (ANA group) significantly promoted the blastocyst formation, and the ANA group was the best treatment of 5-aza-dC to enhance the development of cloned embryos. Read More

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http://dx.doi.org/10.1016/j.rvsc.2020.06.020DOI Listing

cRGD mediated redox and pH dual responsive poly(amidoamine) dendrimer-poly(ethylene glycol) conjugates for efficiently intracellular antitumor drug delivery.

Colloids Surf B Biointerfaces 2020 Jun 12;194:111195. Epub 2020 Jun 12.

Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China. Electronic address:

To improve antitumor efficiency of chemotherapy and reduce side effect, according to the physiological characteristics of tumor tissues and tumor intracellular microenvironment, a multifunctional drug delivery system with properties of long circulation, active targeting, redox and pH triggered drug release was established based on the Generation 4 polyamidoamine dendrimer (PAMAM). First, the redox cleavable disulfide bonds (SS) were introduced for linking polyethylene glycol (PEG) with PAMAM to form PAMAM-S-S-PEG (PSSP). Then cRGD peptide was applied to the PEG end of PSSP to construct RGD-PSSP conjugates. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2020.111195DOI Listing

Real world outcomes in KRAS G12C mutation positive non-small cell lung cancer.

Lung Cancer 2020 Jun 26;146:310-317. Epub 2020 Jun 26.

Department of Medical Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, 3010, Australia.

Background: KRAS mutations are found in 20-30 % of non-small cell lung cancers (NSCLC) and were traditionally considered undruggable. KRAS mutation confers sensitivity to KRAS covalent inhibitors, however its prognostic impact remains unclear. This study assesses the frequency, clinical features, prevalence of brain metastases and outcomes in KRAS NSCLC in a real-world setting. Read More

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http://dx.doi.org/10.1016/j.lungcan.2020.06.030DOI Listing

Peripheral cytokines are not influenced by the type of surgical approach for non-small cell lung cancer by four weeks postoperatively.

Lung Cancer 2020 Jun 27;146:303-309. Epub 2020 Jun 27.

Department of Thoracic and Cardiovascular Surgery, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, United States. Electronic address:

Objectives: The influence of surgical approach on systemic inflammatory response and the subsequent oncologic impact for non-small cell lung cancer is debated. We aimed to measure the effects of thoracic surgical approach on peripheral cytokine milieu over time.

Methods: Patients undergoing primary lung resection without neoadjuvant therapy (2016-2018) were evaluated. Read More

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http://dx.doi.org/10.1016/j.lungcan.2020.06.022DOI Listing

Characteristics and outcomes of lung cancer in solid organ transplant recipients.

Lung Cancer 2020 Jun 20;146:297-302. Epub 2020 Jun 20.

Columbia University Medical Center, Herbert Irving Pavilion, 161 Fort Washington Avenue, New York, NY 10032, United States; Department of Medicine, Columbia University Medical Center, United States. Electronic address:

Objectives: Lung cancer is the third most common malignancy that develops in patients following solid organ transplantation and is the leading cause of cancer deaths in the general population. The aims of this study are to examine the characteristics of patients who developed lung cancer following solid organ transplantation at our institution and to compare their outcomes to those of lung cancer patients without a history of transplant.

Materials And Methods: We performed a single-institution retrospective study of 44 solid organ transplant recipients who developed lung cancer and compared their characteristics to a cohort of 74 lung cancer patients without a history of transplant. Read More

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http://dx.doi.org/10.1016/j.lungcan.2020.06.018DOI Listing

High-throughput proteotyping of bacterial isolates by double barrel chromatography-tandem mass spectrometry based on microplate paramagnetic beads and phylopeptidomics.

J Proteomics 2020 Jun 30:103887. Epub 2020 Jun 30.

Laboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, F-30207 Bagnols sur Cèze, France. Electronic address:

Tandem mass spectrometry-based proteotyping of microorganisms presents several advantages over whole-cell MALDI-TOF mass spectrometry: because a larger number of signals are recorded with better accuracy and precision, the approach allows for the identification of microorganisms at more resolved taxonomic levels, and can easily manage complex samples. Additionally, the use of SP3 paramagnetic beads for cell lysis and protein cleanup simplifies sample preparation for proteotyping. Based on these features, we have developed and tested a 96-well plate platform for high-throughput proteotyping of a large variety of bacteria. Read More

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http://dx.doi.org/10.1016/j.jprot.2020.103887DOI Listing

Delivery of self-amplifying mRNA vaccines by cationic lipid nanoparticles: The impact of cationic lipid selection.

J Control Release 2020 Jun 30. Epub 2020 Jun 30.

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral St., G4 0RE Glasgow, Scotland. Electronic address:

Self-amplifying RNA (SAM) represents a versatile tool that can be used to develop potent vaccines, potentially able to elicit strong antigen-specific humoral and cellular-mediated immune responses to virtually any infectious disease. To protect the SAM from degradation and achieve efficient delivery, lipid nanoparticles (LNPs), particularly those based on ionizable amino-lipids, are commonly adopted. Herein, we compared commonly available cationic lipids, which have been broadly used in clinical investigations, as an alternative to ionizable lipids. Read More

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http://dx.doi.org/10.1016/j.jconrel.2020.06.027DOI Listing

The HDAC6 inhibitor 7b induces BCR-ABL ubiquitination and downregulation and synergizes with imatinib to trigger apoptosis in chronic myeloid leukemia.

Pharmacol Res 2020 Jun 30:105058. Epub 2020 Jun 30.

Department of Pharmacy, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea. Electronic address:

Despite the discovery of tyrosine kinase inhibitors (TKIs) for the treatment of breakpoint cluster region-Abelson (BCR-ABL) cancer types, patients with chronic myeloid leukemia (CML) treated with TKIs develop resistance and severe adverse effects. Combination treatment, especially with a histone deacetylase (HDAC) 6 inhibitor (HDAC6i), appears to be an attractive option to prevent TKI resistance, considering the potential capacity of an HDAC6i to diminish BCR-ABL expression. We first validated the in vivo anti-cancer potential of the compound 7b by significantly reducing the tumor burden of BALB/c mice xenografted with K-562 cells, without notable organ toxicity. Read More

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http://dx.doi.org/10.1016/j.phrs.2020.105058DOI Listing
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