6,983,731 results match your criteria Cell & developmental biology[Journal]

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Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population.

Clin Exp Dent Res 2020 Jul 11. Epub 2020 Jul 11.

Oral and Maxillofacial Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Background: Undifferentiated pleomorphic sarcomas are one of the most common subtypes of soft tissue sarcomas. These are aggressive mesenchymal tumors and are devoid of the major known biomarkers except vimentin. Our objective was to establish and characterize a primary cell population from a mandibular UPS specimen. Read More

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http://dx.doi.org/10.1002/cre2.301DOI Listing

Regenerative effects of human chondrocyte sheets in a xenogeneic transplantation model using immune-deficient rats.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan.

Although cell transplantation has attracted much attention in regenerative medicine, animal models continue to be used in translational research to evaluate safety and efficacy because cell sources and transplantation modalities are so diverse. In the present study, we investigated the regenerative effects of human chondrocyte sheets on articular cartilage in a xenogeneic transplantation model using immune-deficient rats. Osteochondral defects were created in the knee joints of immune-deficient rats that were treated as: Group A, untreated (without transplantation); Group B, transplantation of a layered chondrocyte sheet containing 5. Read More

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http://dx.doi.org/10.1002/term.3101DOI Listing

Two novel mutations in the MCM8 gene shared by two Chinese siblings with primary ovarian insufficiency and short stature.

Mol Genet Genomic Med 2020 Jul 11:e1396. Epub 2020 Jul 11.

Department of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: Minichromosome maintenance complex component 8 (MCM8) is responsible for homologous recombination and DNA double-strand breaks (DSBs) repair and is the cause of primary ovarian insufficiency (POI), which is seldom diagnosed in adolescents and children.

Methods: Whole-exome sequencing was performed in a 13-year-old girl, and Sanger sequencing was used to identify potentially pathogenic variants in her sister (aged 6 years and 7 months) and parents. To identify potential pathogenic mutations, DSBs were induced by mitomycin C (MMC), and the DNA repair capacity was evaluated by the histone H2AX phosphorylation level. Read More

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http://dx.doi.org/10.1002/mgg3.1396DOI Listing

Caffeine Upregulates Hepatic Sex Hormone-Binding Globulin Production by Increasing Adiponectin Through AKT/FOXO1 Pathway in White Adipose Tissue.

Mol Nutr Food Res 2020 Jul 11:e1901253. Epub 2020 Jul 11.

Diabetes and Metabolism Research Unit, Vall Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona and CIBERDEM (ISCIII), Barcelona, Spain.

Scope: Epidemiological studies have shown that caffeine increases serum sex hormone-binding globulin (SHBG) levels. The relationship between caffeine and SHBG production has never been studied before at molecular level. The aim of our study was to examine whether caffeine regulates SHBG production and to determine the associated molecular mechanisms. Read More

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http://dx.doi.org/10.1002/mnfr.201901253DOI Listing

Ageing-associated increase in SGLT2 disrupts mitochondrial/sarcoplasmic reticulum Ca homeostasis and promotes cardiac dysfunction.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Departments of Biophysics, Ankara University Faculty of Medicine, Ankara, Turkey.

The prevalence of death from cardiovascular disease is significantly higher in elderly populations; the underlying factors that contribute to the age-associated decline in cardiac performance are poorly understood. Herein, we identify the involvement of sodium/glucose co-transporter gene (SGLT2) in disrupted cellular Ca -homeostasis, and mitochondrial dysfunction in age-associated cardiac dysfunction. In contrast to younger rats (6-month of age), older rats (24-month of age) exhibited severe cardiac ultrastructural defects, including deformed, fragmented mitochondria with high electron densities. Read More

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http://dx.doi.org/10.1111/jcmm.15483DOI Listing

Enzyme Instructed Self-assembly of Naphthalimide-dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation.

Chem Asian J 2020 Jul 11. Epub 2020 Jul 11.

National Chiao Tung University, Materials Science and Engineering, 1001 University Rd., 30010, Hsinchu, TAIWAN.

Understanding the structure-morphology relationships of the self-assembled nanostructures is very crucial for developing the materials with the desired chemical and biological functions. Here, phosphate-based naphthalimide (NI) derivatives have been developed for the first time to study the enzyme instructed self-assembly process. Self-assembly of simple amino acid derivative NI-Y p resulted in non-specific amorphous aggregates in the presence of alkaline phosphatase enzyme. Read More

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http://dx.doi.org/10.1002/asia.202000575DOI Listing

MMP13 and TIMP1 are functional markers for two different potential modes of action by mesenchymal stem/stromal cells when treating osteoarthritis.

Stem Cells 2020 Jul 11. Epub 2020 Jul 11.

Institute of Lifecourse and Medical Sciences, University of Liverpool, UK.

Mesenchymal stem cells (MSCs) have been investigated as a potential injectable therapy for the treatment of knee osteoarthritis, with some evidence of success in preliminary human trials. However, optimisation and scale-up of this therapeutic approach depends on the identification of functional markers that are linked to their mechanism of action. One possible mechanism is through their chondrogenic differentiation and direct role in neo-cartilage synthesis. Read More

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http://dx.doi.org/10.1002/stem.3255DOI Listing

LINC00160 mediated paclitaxel-And doxorubicin-resistance in breast cancer cells by regulating TFF3 via transcription factor C/EBPβ.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Center for Precision Medicine, Anhui No.2 Provincial People's Hospital, Hefei, China.

Chemoresistance represents a major challenge in breast cancer (BC) treatment. This study aimed to probe the roles of LINC00160 in paclitaxel- and doxorubicin-resistant BC cells. Three pairs of BC and adjacent normal tissue were used for lncRNA microarray analysis. Read More

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http://dx.doi.org/10.1111/jcmm.15487DOI Listing

Significance of tumor microenvironment in acquiring resistance to vascular endothelial growth factor-tyrosine kinase inhibitor and recent advance of systemic treatment of clear cell renal cell carcinoma.

Pathol Int 2020 Jul 11. Epub 2020 Jul 11.

Department of Urology, Keio University School of Medicine, Tokyo, Japan.

The development of systemic therapies, including vascular endothelial growth factor-tyrosine kinase inhibitors (VEGF-TKI) and mammalian target of rapamycin (mTOR) inhibitors, represents a major breakthrough in the treatment of patients with renal cell carcinoma (RCC). However, inherent resistance is observed in some patients and acquired resistance commonly develops in many patients within several months of the initiation of systemic therapies. Since these treatments rarely cure patients, their aim is to suppress tumor progression and prolong survival. Read More

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http://dx.doi.org/10.1111/pin.12984DOI Listing

Knockdown of DNA binding protein A (dbpA) enhances the chemotherapy sensitivity of colorectal cancer via suppressing the Wnt/β-catenin/Chk1 pathway.

Cell Biol Int 2020 Jul 11. Epub 2020 Jul 11.

Department of hepatobiliary surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P.R. China.

DNA-binding protein A (dbpA) is reported to be upregulated in many cancers and associated with tumor progress. The present study aimed to investigate the role of dbpA in 5-Fluorouracil (5-FU)-resistant and Oxaliplatin (L-OHP)-resistant colorectal cancer (CRC) cells. We found that 5-FU and L-OPH treatment promoted the expression of dbpA. Read More

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http://dx.doi.org/10.1002/cbin.11416DOI Listing

The Response of Gingiva Monolayer, Spheroid and ex vivo Tissue Cultures to Collagen Membranes and Bone Substitute.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Department of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.

Collagen membranes and bone substitute are popular biomaterials in guided tissue regeneration for treatment of traumatized or diseased periodontal tissue. Development of these biomaterials starts in monolayer cell culture, failing to reflect in vivo tissue organization. Spheroid cultures potentially mimic in vivo tissues in structure and functionality. Read More

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http://dx.doi.org/10.1002/term.3102DOI Listing

Genome-wide admixture mapping of DSM-IV alcohol dependence, criterion count, and the self-rating of the effects of ethanol in African American populations.

Am J Med Genet B Neuropsychiatr Genet 2020 Jul 11. Epub 2020 Jul 11.

Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana, USA.

African Americans (AA) have lower prevalence of alcohol dependence and higher subjective response to alcohol than European Americans. Genome-wide association studies (GWAS) have identified genes/variants associated with alcohol dependence specifically in AA; however, the sample sizes are still not large enough to detect variants with small effects. Admixture mapping is an alternative way to identify alcohol dependence genes/variants that may be unique to AA. Read More

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http://dx.doi.org/10.1002/ajmg.b.32805DOI Listing

SNPs in SNCA, MCCC1, DLG2, GBF1 and MBNL2 are associated with Parkinson's disease in southern Chinese population.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Department of Neurology and Institute of Neurology, Ruijin Hospital Affiliated with the Shanghai Jiaotong University School of Medicine, Shanghai, China.

Numerous single nucleotide polymorphisms (SNPs), which have been identified as susceptibility factors for Parkinson's disease (PD) as per genome-wide association studies, have not been fully characterized for PD patients in China. This study aimed to replicate the relationship between 12 novel SNPs of 12 genes and PD risk in southern Chinese population. Twelve SNPs of 12 genes were detected in 231 PD patients and 249 controls, using the SNaPshot technique. Read More

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http://dx.doi.org/10.1111/jcmm.15508DOI Listing

Pyroglutamylated RFamide peptide 43: a putative modulator of testicular steroidogenesis.

Andrology 2020 Jul 11. Epub 2020 Jul 11.

Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.

Background: We have recently shown that QRFP and its receptor are predominantly expressed in germ cells, Sertoli cells, and Leydig cells in mice testes.

Objective: The present study investigated the role of QRFP in testicular steroidogenesis in mice.

Materials And Methods: Both ex vivo and in vivo experiments were performed. Read More

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http://dx.doi.org/10.1111/andr.12864DOI Listing

The impact of antibiotic allergy testing in transplant patients.

Transpl Infect Dis 2020 Jul 11:e13411. Epub 2020 Jul 11.

Department of Infectious Diseases and Centre for Antibiotic Allergy and Research, Austin Health,, VIC, Australia.

Patient reported antibiotic allergies, so-called antibiotic allergy labels (AAL) are highly prevalent amongst hospitalized patients globally . The highest prevalence of AALs has been found to be in immunocompromised patients, in particular transplant patients, with a large U.S. Read More

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http://dx.doi.org/10.1111/tid.13411DOI Listing

Acylglycerol kinase promotes the stemness of nasopharyngeal carcinoma cells by promoting β-catenin translocation to the nucleus through activating PI3K/Akt pathway.

Environ Toxicol 2020 Jul 11. Epub 2020 Jul 11.

Department of Otolaryngology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, China.

Recent evidences show that acylglycerol kinase (AGK) expression is related to the occurrence and development of various human cancers. However, its roles in nasopharyngeal carcinoma (NPC) progression are still unclear. This work aims to explore the roles of AGK in NPC cell stemness. Read More

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http://dx.doi.org/10.1002/tox.22994DOI Listing

Priming of microglia with IFN-γ impairs adult hippocampal neurogenesis and leads to depression-like behaviors and cognitive defects.

Glia 2020 Jul 11. Epub 2020 Jul 11.

School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.

Neuroinflammation driven by interferon-gamma (IFN-γ) and microglial activation has been linked to neurological disease. However, the effects of IFN-γ-activated microglia on hippocampal neurogenesis and behavior are unclear. In the present study, IFN-γ was administered to mice via intracerebroventricular injection. Read More

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http://dx.doi.org/10.1002/glia.23878DOI Listing

Administration of secretome from human placental stem cell conditioned media improves recovery of erectile function in the pelvic neurovascular injury model.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.

Introduction: Human placental stem cells (PSC) enhance histological and functional recovery in a rodent erectile dysfunction (ED) model. We tested the hypothesis that bioactive factors secreted by PSC (i.e. Read More

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http://dx.doi.org/10.1002/term.3105DOI Listing

Treatment of a large osseous defect in a feline tarsus using a stem cell-seeded custom implant.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Fitzpatrick Referrals, Eashing, Godalming, Surrey, United Kingdom.

Aim: To describe the treatment of an infected segmental bone defect in a cat using a novel, custom-designed titanium implant seeded with adipose-derived stem cells (AdMSCs) to facilitate osseous ingrowth and preserve limb function.

Case Report: Large bone defects occur secondary to trauma, infection, or neoplasia, and often result in amputation. We established a novel autologous AdMSC-impregnated trabecular metal spacer made using 3D printing, to bridge the distal tibia and metatarsal bones in the left pelvic limb of a cat that had previously undergone right pelvic limb amputation. Read More

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http://dx.doi.org/10.1002/term.3104DOI Listing

Clearance of therapy-induced senescent tumor cells by the senolytic ABT-263 via interference with BCL-X -BAX Interaction.

Mol Oncol 2020 Jul 11. Epub 2020 Jul 11.

Departments of Pharmacology & Toxicology, School of Medicine, Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, USA.

Tumor cells undergo senescence in response to both conventional and targeted cancer therapy. The induction of senescence in response to cancer therapy can contribute to unfavorable outcomes, potentially including cancer relapse. This work demonstrates that tumor cells induced into senescence by doxorubicin, etoposide, or radiation can give rise to viable tumors in vivo. Read More

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http://dx.doi.org/10.1002/1878-0261.12761DOI Listing

Sotrastaurin, a PKC inhibitor, attenuates RANKL-induced bone resorption and attenuates osteochondral pathologies associated with the development of OA.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Department of Orthopedics, The Ninth Affiliated Hospital of Guangxi Medical University, Beihai, China.

Osteoarthritis (OA) is a common degenerative disease that affects the musculoskeletal structure of the whole joint, which is characterized by progressive destruction of both articular cartilage and subchondral bone. Treatment of the bone pathologies, particularly osteoclast-mediated subchondral bone loss in the early stages of OA, could prevent subsequent cartilage degeneration and progression of OA. In the present study, the PKC inhibitor, Sotrastaurin, was found to inhibit RANKL-induced osteoclast formation in vitro in a dose- and time-dependent manner. Read More

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http://dx.doi.org/10.1111/jcmm.15404DOI Listing

Potentiation of calcium-activated chloride secretion and barrier dysfunction may underlie EGF receptor tyrosine kinase inhibitor-induced diarrhea.

Physiol Rep 2020 Jul;8(13):e14490

Division of Gastroenterology, Department of Medicine, University of California San Diego, La Jolla, CA, USA.

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFr TKIs) are first-line therapies for various cancers, and cause dose-limiting severe diarrhea in many patients. We hypothesized that diarrhea caused by EGFr TKIs might reflect actions on epithelial transport, barrier function, or both, which we tested using cell cultures including murine and human enteroid-derived monolayers (EDMs), analyzed using electrophysiological and other relevant methods. EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Read More

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http://dx.doi.org/10.14814/phy2.14490DOI Listing

Topical RT1640 treatment effectively reverses gray hair and stem cell loss in a mouse model of radiation-induced canities.

Pigment Cell Melanoma Res 2020 Jul 11. Epub 2020 Jul 11.

Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.

Gray hair is a visible sign of tissue degeneration during aging. Graying is attributed to dysfunction of melanocyte stem cells (McSCs) that results in depletion of their melanin-producing progeny. This non-lethal phenotype makes the hair follicle and its pigment system an attractive model for investigating mechanisms that contribute to tissue aging as well as therapeutic strategies to combat this process. Read More

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http://dx.doi.org/10.1111/pcmr.12913DOI Listing

Increased Inflammasome Activity in Subcutaneous Adipose Tissue of Patients with Metabolic Syndrome.

Diabetes Metab Res Rev 2020 Jul 11. Epub 2020 Jul 11.

VA Medical Center, Mather, California.

Aims: The metabolic syndrome (MetS) is an inflammatory disorder associated with an increased risk for diabetes and atherosclerotic cardiovascular disease (ASCVD). Studies in patients and animal models of obesity and diabetes have shown increased NOD-like receptor family pyrin domain containing 3 (NLPR3) inflammasome activity. However, there is scanty data on the activity of the NLRP3 inflammasome in patients with nascent MetS. Read More

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http://dx.doi.org/10.1002/dmrr.3383DOI Listing

De novo mutation and skewed X-inactivation in girl with BCAP31-related syndrome.

Hum Mutat 2020 Jul 11. Epub 2020 Jul 11.

Departments of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.

Full genome analysis of a young girl with deafness, dystonia, central hypomyelination, refractory seizure, and fluctuating liver function impairment revealed a heterozygous, de novo variant in the BCAP31 gene on chromosome Xq28 (NM_001256447.2:c.92G>A), mutations of which caused the X-linked recessive severe neurologic disorder DDCH (Deafness, Dystonia, and Cerebral Hypomyelination). Read More

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http://dx.doi.org/10.1002/humu.24080DOI Listing

Decellularised human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction.

J Tissue Eng Regen Med 2020 Jul 11. Epub 2020 Jul 11.

Laboratory of Experimental Ophthalmology, Department of Ophthalmology, Pius-Hospital, Carl von Ossietzky University, Germany.

The shortage of donor corneas as well as the limitations of tissue substitutes leads to the necessity to develop alternative materials for ocular surface reconstruction. Corneal surface substitutes must fulfill specific requirements such as high transparency, low immunogenicity, and mechanical stability combined with elasticity. This in vitro study evaluates a decellularized matrix secreted from human corneal fibroblast (hCF) as an alternative material for ocular surface reconstruction. Read More

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http://dx.doi.org/10.1002/term.3103DOI Listing

Low-magnitude vibration induces osteogenic differentiation of bone marrow mesenchymal stem cells via miR-378a-3p/Grb2 pathway to promote bone formation in a rat model of age-related bone loss.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

The dysfunction of bone marrow mesenchymal stem cells (BMSCs) in osteogenic differentiation is one of the main causes of age-related bone loss. Our previous studies have shown that low-magnitude vibration (LMV) induces the osteogenic differentiation of BMSCs derived from ovariectomized osteoporotic rats. To investigate whether LMV promotes osteogenic differentiation of BMSCs and its underlying mechanisms in aged rats, 20-month-old female Sprague-Dawley rats (n = 20) were randomly divided into LMV group (rats were vibrated at 0. Read More

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http://dx.doi.org/10.1096/fj.201902830RRRDOI Listing

Metabolic implication of tigecycline as an efficacious second-line treatment for sorafenib-resistant hepatocellular carcinoma.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Institute of Pharmacy, Center for Public Health, Paracelsus Medical University, Salzburg, Austria.

Sorafenib represents the current standard of care for patients with advanced-stage hepatocellular carcinoma (HCC). However, acquired drug resistance occurs frequently during therapy and is accompanied by rapid tumor regrowth after sorafenib therapy termination. To identify the mechanism of this therapy-limiting growth resumption, we established robust sorafenib resistance HCC cell models that exhibited mitochondrial dysfunction and chemotherapeutic crossresistance. Read More

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http://dx.doi.org/10.1096/fj.202001128RDOI Listing

The link between Vitamin D and Covid-19: distinguishing facts from fiction.

Authors:
Peter Bergman

J Intern Med 2020 Jul 11. Epub 2020 Jul 11.

Department of Laboratory Medicine, Karolinska Institutet, Alfred Nobels Alle 8, 141 52, Huddinge, Sweden.

Vitamin D is produced in the skin under the influence of UVB-light from the sun or obtained via the diet by eating fatty fish, enriched dairy products or supplements. Vitamin D is known to support a healthy bone and severe deficiency may lead to osteomalacia or the rickets, which still occur in poor areas of the world. In addition, vitamin D support key functions in many organs, including the brain, muscle and the immune systems (Holick, 2007). Read More

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http://dx.doi.org/10.1111/joim.13158DOI Listing

MIB1-mediated degradation of WRN promotes cellular senescence in response to camptothecin treatment.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Department of Medical Genetics, Center for Medical Genetics, Peking University Health Science Center, Beijing, China.

Werner syndrome protein (WRN) plays critical roles in DNA replication, recombination, and repair, as well as transcription and cellular senescence. Ubiquitination and degradation of WRN have been reported, however, the E3 ubiquitin ligase of WRN is little known. Here, we identify mindbomb E3 ubiquitin protein ligase 1 (MIB1) as a novel E3 ubiquitin ligase for WRN protein. Read More

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http://dx.doi.org/10.1096/fj.202000268RRRDOI Listing

Nuclear β-catenin immunoexpression in scars.

J Cutan Pathol 2020 Jul 11. Epub 2020 Jul 11.

Department of Diagnostic Pathology, Shizuoka Cancer Center Hospital, Nagaizumi, Japan.

Background: Histopathologically, scars can mimic superficial fibromatoses. Superficial fibromatoses are known to show nuclear β-catenin immunoexpression, although the tumor types do not harbor CTNNB1 or APC alterations. The present study aimed to evaluate nuclear β-catenin immunoexpression in scars compared to that in superficial fibromatoses. Read More

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http://dx.doi.org/10.1111/cup.13806DOI Listing

Mitochondrial division inhibitor 1 (mdivi-1) increases oxidative capacity and contractile stress generated by engineered skeletal muscle.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Laboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.

In skeletal muscle fibers, mitochondria are densely packed adjacent to myofibrils because adenosine triphosphate (ATP) is needed to fuel sarcomere shortening. However, despite this close physical and biochemical relationship, the effects of mitochondrial dynamics on skeletal muscle contractility are poorly understood. In this study, we analyzed the effects of Mitochondrial Division Inhibitor 1 (mdivi-1), an inhibitor of mitochondrial fission, on the structure and function of both mitochondria and myofibrils in skeletal muscle tissues engineered on micromolded gelatin hydrogels. Read More

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http://dx.doi.org/10.1096/fj.201901039RRDOI Listing

Phylogenetic analysis and expression differences of eye-related genes in cavefish genus Sinocyclocheilus.

Integr Zool 2020 Jul 11. Epub 2020 Jul 11.

State Key Laboratory of Membrane Biology, Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

The adaptive evolution of visual systems has been observed in many cavefish. However, little is known about the molecular mechanisms underlying these adaptations, which include regressive changes such as eye degeneration. Here, we analyzed phylogenetic and expression patterns of six eye-related genes (crx, foxg1b, opn1sw2, otx2, rho, and sox2) in twelve Sinocyclocheilus species from China including eight stygobionts and four stygophiles, and examined photoreceptor cell morphology of these species. Read More

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http://dx.doi.org/10.1111/1749-4877.12466DOI Listing

MicroRNA-142 regulates osteoblast differentiation and apoptosis of mouse pre-osteoblast cells by targeting bone morphogenetic protein 2.

FEBS Open Bio 2020 Jul 11. Epub 2020 Jul 11.

Department of Anesthesiology, Weifang People's Hospital, Weifang, Shandong Province, 261041, P. R. China.

Osteoporosis is a common disease that can seriously impair the physical and mental health of the elderly. However, current treatment is unsatisfactory, due to the lack of effective therapeutic targets. Abnormal expression and involvement of microRNA-142 (miR-142) have been identified in many diseases, including bone-related diseases. Read More

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http://dx.doi.org/10.1002/2211-5463.12929DOI Listing

DNMT3B deficiency alters mitochondrial biogenesis and α-ketoglutarate levels in human embryonic stem cells.

Stem Cells 2020 Jul 11. Epub 2020 Jul 11.

Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo and Oslo University Hospital, Oslo, Norway.

Embryonic stem cell (ESC) renewal and differentiation is regulated by metabolites that serve as co-factors for epigenetic enzymes. Increase of α-ketoglutarate (α-KG), a co-factor for histone and DNA demethylases, triggers multi-lineage differentiation in human ESCs. To gain further insight how the metabolic fluxes in pluripotent stem cells can be influenced by inactivating mutations in epigenetic enzymes, we generated human ESCs deficient for de novo DNA methyltransferases (DNMT) 3A and 3B. Read More

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http://dx.doi.org/10.1002/stem.3256DOI Listing

A cholesterosis of the common bile duct successfully diagnosed macroscopically and histologically using the transpapillary approach.

Dig Endosc 2020 Jul 11. Epub 2020 Jul 11.

Division of Gastroenterology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8575, Japan.

Cholesterosis is defined as the aggregation of foamy cells under the mucosa [1]. Although cholesterosis of the gallbladder is a common disease, cholesterosis of the common bile duct (CBD) is rare, with only <40 cases reported [2], and diagnosis is extremely difficult [3]. Since cholesterosis is basically a benign tumor, a direct visual examination and histopathological diagnosis is important to reduce unnecessary surgery [4], and endoscopic retrograde cholangiopancreatography (ERCP) and its related procedure have great benefits for patients because of its less-invasiveness and simplicity. Read More

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http://dx.doi.org/10.1111/den.13790DOI Listing

Positive selection and convergent evolution shape molecular phenotypic traits of innate immunity receptors in tits (Paridae).

Mol Ecol 2020 Jul 11. Epub 2020 Jul 11.

Charles University, Faculty of Science, Department of Zoology, Viničná 7, 128 43, Prague, Czech Republic.

Despite widespread variability and redundancy abounding animal immunity, little is currently known about the rate of evolutionary convergence (functionally analogous traits not inherited from a common ancestor) in host molecular adaptations to parasite selective pressures. Toll-like receptors (TLRs) provide the molecular interface allowing hosts to recognize pathogenic structures and trigger early danger signals initiating an immune response. Using a novel combination of bioinformatic approaches, here we explore genetic variation in ligand-binding regions of bacteria-sensing TLR4 and TLR5 in 29 species belonging to the tit family of passerine birds (Aves: Paridae). Read More

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http://dx.doi.org/10.1111/mec.15547DOI Listing

Astroglial N-myc downstream-regulated gene 2 protects the brain from cerebral edema induced by stroke.

Glia 2020 Jul 11. Epub 2020 Jul 11.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, The Air Force Military Medical University, Xi'an, China.

Brain edema is a grave complication of brain ischemia and is the main cause of herniation and death. Although astrocytic swelling is the main contributor to cytotoxic edema, the molecular mechanism involved in this process remains elusive. N-myc downstream-regulated gene 2 (NDRG2), a well-studied tumor suppressor gene, is mainly expressed in astrocytes in mammalian brains. Read More

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http://dx.doi.org/10.1002/glia.23888DOI Listing

CURLED LATER1 encoding the largest subunit of the Elongator complex has a unique role in leaf development and meristem function in rice.

Plant J 2020 Jul 11. Epub 2020 Jul 11.

School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-8654, Japan.

The Elongator complex, which is conserved in eukaryotes, has multiple roles in diverse organisms. In Arabidopsis thaliana, Elongator is shown to be involved in development, hormone action, and environmental responses. Except for Arabidopsis, however, our knowledge of its function is poor in plants. Read More

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http://dx.doi.org/10.1111/tpj.14925DOI Listing

Surgical results and pathological analysis of cardiac fibroma in the adolescent and the adult.

J Card Surg 2020 Jul 11. Epub 2020 Jul 11.

National Clinical Research Center of Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Backgrounds: Disparities may exist between the adolescent and the adult patients with cardiac fibromas in the symptoms, surgical outcomes, and pathological characteristics. The aim of this study was to compare short and midterm surgical outcomes of cardiac fibromas and to compare the biomarker expressions of tumor tissue samples between the adult and the adolescent.

Methods: Consecutive patients with the diagnosis of cardiac fibroma were admitted and received surgeries. Read More

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http://dx.doi.org/10.1111/jocs.14790DOI Listing

Elevated In Vitro Kinase Activity in Peripheral Blood Mononuclear Cells of Leucine-Rich Repeat Kinase 2 G2019S Carriers: A Novel Enzyme-Linked Immunosorbent Assay-Based Method.

Mov Disord 2020 Jul 11. Epub 2020 Jul 11.

Division of Basic Neurosciences, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.

Background: Leucine-rich repeat kinase 2 kinase inhibitors are being vigorously pursued as potential therapeutic options; however, there is a critical need for sensitive and quantitative assays of leucine-rich repeat kinase 2 function and target engagement.

Objectives: Our objective was to compare collection and storage protocols for peripheral blood mononuclear cells, and to determine the optimal conditions for downstream analyses of leucine-rich repeat kinase 2 in PD cohorts.

Methods: Here, we describe enzyme-linked immunosorbent assay-based assays capable of detecting multiple aspects of leucine-rich repeat kinase 2 function at endogenous levels in human tissues. Read More

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http://dx.doi.org/10.1002/mds.28175DOI Listing

Aldosterone-induced microRNAs act as feedback regulators of mineralocorticoid receptor signaling in kidney epithelia.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA.

The final steps in the Renin-Angiotensin-Aldosterone signaling System (RAAS) involve binding of the corticosteroid hormone, aldosterone to its mineralocorticoid receptor (MR). The bound MR interacts with response elements to induce or repress the transcription of aldosterone-regulated genes. A well characterized aldosterone-induced gene is the serum and glucocorticoid-induced kinase (SGK1), which acts downstream to increase sodium transport in distal kidney nephron epithelial cells. Read More

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http://dx.doi.org/10.1096/fj.201902254RRDOI Listing

Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A).

Hum Mutat 2020 Jul 11. Epub 2020 Jul 11.

Brain and Mitochondrial Research Group, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia.

Defects in the motor domain of kinesin family member 1A (KIF1A), a neuron-specific ATP-dependent anterograde axonal transporter of synaptic cargo, are well-recognized to cause a spectrum of neurological conditions, commonly known as KIF1A-associated neurological disorders (KAND). Here we report one mutation-negative female with classic Rett syndrome (RTT) harboring a de novo heterozygous novel variant [NP_001230937.1:p. Read More

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http://dx.doi.org/10.1002/humu.24079DOI Listing

Mouse Double Minute 2 Amplification in Esophageal Squamous Cell Carcinoma Associated with Better Outcome.

Histopathology 2020 Jul 11. Epub 2020 Jul 11.

Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.

Aims: This study aimed to determine the frequency of mouse double minute 2 (MDM2) amplification in esophageal squamous cell carcinomas (ESCC) and to clarify its prognostic significance.

Methods: We investigated MDM2 amplification on tissue microarrays, using fluorescence in situ hybridization and analyzed its correlations with clinicopathological features and outcomes in 515 Chinese ESCC patients.

Results: MDM2 amplifications were found in 37 of 515 ESCC patients (7. Read More

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http://dx.doi.org/10.1111/his.14208DOI Listing

Epithelial/mesenchymal heterogeneity of high-grade serous ovarian carcinoma samples correlates with miRNA let-7 levels and predicts tumor growth and metastasis.

Mol Oncol 2020 Jul 11. Epub 2020 Jul 11.

Department of Basic Sciences, Division of Biochemistry, Loma Linda University, Loma Linda, CA, USA.

Patient-derived samples present an advantage over current cell line models of high grade serous ovarian cancer (HGSOC) that are not always reliable and phenotypically faithful models of in vivo HGSOC. To improve upon cell line models of HGSOC, we set out to characterize a panel of patient-derived cells and determine their epithelial and mesenchymal characteristics. We analyzed RNA and protein expression levels in patient-derived xenograft (PDX) models of HGSOC, and functionally characterized these models using flow cytometry, wound healing assays, invasion assays and spheroid cultures. Read More

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http://dx.doi.org/10.1002/1878-0261.12762DOI Listing

Aortopulmonary paraganglioma masquerading as an anterior mediastinal mass: A fine line between confusion and chaos!

J Card Surg 2020 Jul 11. Epub 2020 Jul 11.

Department of Cardiothoracic Surgery, The Essex Cardiothoracic Centre, Basildon University Hospital, Nether Mayne, Basildon, UK.

Mediastinal paragangliomas are exceedingly rare neuroendocrine tumors of chromaffin cell origin. They are rarely endocrinologically functional, but complications often arise due to mass effect within the mediastinal cavity. We present a case of a 67-year-old gentleman referred to our unit for excision of a large mediastinal mass, thought to be thymic in origin, but without confirmatory preoperative histological diagnosis. Read More

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http://dx.doi.org/10.1111/jocs.14751DOI Listing

Serum progranulin levels in pediatric patients with Gaucher disease; relation to disease severity and liver stiffness by transient elastography.

Liver Int 2020 Jul 11. Epub 2020 Jul 11.

Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Background: Non-invasive screening for liver fibrosis using transient elastography (TE) could be of value in the management of Gaucher disease (GD). Progranulin (PGRN) is a novel disease modifier in GD and an independent marker of liver fibrosis.

Objectives: We determined PGRN levels in pediatric patients with GD and assessed its role as a potential marker for disease severity and relation to liver stiffness by TE. Read More

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http://dx.doi.org/10.1111/liv.14598DOI Listing

Daratumumab for disabling cold agglutinin disease refractory to B-cell directed therapy.

Am J Hematol 2020 Jul 11. Epub 2020 Jul 11.

Department of Haematology, University College London Hospitals NHS Foundation Trust, London, United Kingdom.

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http://dx.doi.org/10.1002/ajh.25932DOI Listing

Long Term Follow Up of Hematopoietic Stem Cell Transplant Survivors: A Focus on Screening, Monitoring, and Therapeutics.

Pharmacotherapy 2020 Jul 11. Epub 2020 Jul 11.

University of California, San Francisco Medical Center, Department of Pharmaceutical Services, San Francisco, CA, USA.

Annually, approximately 50,000 patients undergo hematopoietic stem cell transplantation (HCT) worldwide with almost 22,000 of these patients receiving HCT in the United States. HCT is a curative option for a wide range of hematologic malignancies and advances in transplantation medicine have resulted in an increase in HCT survivors. It is anticipated that the number of HCT survivors will more than double from 242,000 in 2020 to approximately 500,000 in 2030. Read More

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http://dx.doi.org/10.1002/phar.2443DOI Listing

Determination of the immunostimulatory drug - Glucosoaminyl-Muramyl-Dipeptide - in human plasma using high performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study.

Biomed Chromatogr 2020 Jul 11:e4948. Epub 2020 Jul 11.

Laboratory of Pharmacokinetics and Metabolomic Analysis, Institute of Translational Medicine and Biotechnology, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

Introduction: MDP (glucosaminyl-muramyl-dipeptide), a synthetic analog of the peptidoglycan fragment of the bacterial cell wall, is an active component of the immunomodulatory drug Licopid®. But, the pharmacokinetic parameters of GMDP in humans after oral administration have not been investigated yet. A present study aimed to develop and validate a sensitive liquid chromatography-tandem mass spectrometry method for the analysis of GMDP in human plasma. Read More

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http://dx.doi.org/10.1002/bmc.4948DOI Listing
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