71 results match your criteria Carnets De Geologie[Journal]


Assessing T follicular helper cell function in vivo: antigen-specific B cell response to hapten and affinity maturation.

Methods Mol Biol 2015 ;1291:87-101

INSERM UMR_S996, LabEx LERMIT, Université Paris-sud, 32 rue des Carnets, Clamart, 92140, France.

The mechanisms controlling affinity maturation have been extensively studied over the last 20 years and the central role of T follicular helper cells (Tfh) in this process has now been clearly established. In order to analyze how Tfh impact on affinity maturation several models have been developed. This chapter aims to present three different techniques to evaluate antigen-specific B cell response and affinity maturation using the NP system: Flow cytometric single cell sorting and sequence analysis, ELISA and ELISpot. Read More

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http://dx.doi.org/10.1007/978-1-4939-2498-1_8DOI Listing
December 2015
13 Reads

[The new tools of microbiological diagnosis of tuberculosis].

Rev Med Interne 2014 Dec 8;35(12):794-800. Epub 2014 Aug 8.

Service de bactériologie-hygiène, hôpital Antoine-Béclère, AP-HP, HUPS, 157, rue de la Porte-de-Trivaux, 92140 Clamart, France. Electronic address:

This review focuses on the role of new tools in the "modern" microbiological diagnosis of tuberculosis. Traditional techniques of microscopy and culture remain essential to diagnostic certainty, but some innovations replace daily the older techniques such as the identification of Mycobacterium tuberculosis complex by immunochromatography or mass spectrometry MALDI-TOF type from positive cultures, or susceptibility testing in liquid medium. New tools that use molecular techniques have become important. Read More

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http://dx.doi.org/10.1016/j.revmed.2014.05.001DOI Listing
December 2014
1 Read

The relevance of the chemokine receptor ACKR3/CXCR7 on CXCL12-mediated effects in cancers with a focus on virus-related cancers.

Cytokine Growth Factor Rev 2014 Jun 9;25(3):307-16. Epub 2014 May 9.

Univ. Paris-Sud, Laboratoire "Cytokines, Chemokines and Immunopathology", UMR_S996, 32, rue des Carnets, Clamart F-92140, France; INSERM, Univ. Paris-Sud, Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT), Clamart F-92140, France. Electronic address:

Recent studies have highlighted the importance of understanding the molecular determinants of CXCL12-mediated effects in cancers. Once previously thought to interact exclusively with CXCR4, CXCL12 also binds with high affinity to CXCR7 (recently renamed ACKR3), which belongs to an atypical chemokine receptor family whose members fail to activate Gαi proteins but interact with β-arrestins. In addition to its capacity to control CXCL12 bioavailability, ACKR3 can either enhance or dampen CXCR4-mediated signaling and activity. Read More

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http://dx.doi.org/10.1016/j.cytogfr.2014.04.006DOI Listing
June 2014
108 Reads

Loss of LH-induced down-regulation of anti-Müllerian hormone receptor expression may contribute to anovulation in women with polycystic ovary syndrome.

Hum Reprod 2013 Mar 14;28(3):762-9. Epub 2013 Jan 14.

INSERM, U782, 32 rue des Carnets, Clamart F-92140, France.

Study Question: Are anti-Müllerian hormone (AMH) and AMH type II receptor (AMHR-II) mRNAs similarly regulated by gonadotrophins in lutein granulosa cells (GCs) from control, normo-ovulatory and oligo/anovulatory women with polycystic ovary syndrome (PCOS)?

Summary Answer: AMH mRNA expression was induced by LH only in lutein GC of oligo/anovulatory PCOS women; down-regulation of AMHR-II, induced by LH in control and normo-ovulatory PCOS women, was absent in oligo/anovulatory women.

What Is Known Already: It was suggested that AMH could be responsible for the blockade of follicles at the small antral stage in PCOS women. In keeping with this hypothesis, both AMH and AMHR-II are overexpressed in lutein GCs from oligo/anovulatory PCOS women. Read More

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http://dx.doi.org/10.1093/humrep/des460DOI Listing
March 2013
68 Reads

Altered p16 and Bcl-2 expression reflects pathologic development in hydatidiform moles and choriocarcinoma.

Pathol Oncol Res 2013 Apr 13;19(2):217-27. Epub 2012 Oct 13.

INSERM U782 Endocrinologie et génétique de la reproduction et du développement, 32 rue des Carnets, 92140, Clamart, France.

Abnormal trophoblast differentiation is the main cause of gestational trophoblast diseases in the case of hydatidiform moles and choriocarcinomas. Here we investigated the expression patterns of two gene products, p16 and Bcl-2, implicated in cell cycle regulation and apoptosis, respectively, using immunohistochemistry during normal placenta differentiation, hydatidiform moles (partial, complete and invasive) and post-molar choriocarcinomas. The p16 protein shows a gradual expression in cytotrophoblast of normal villous, from a p16 weak proliferative phenotype to a p16 strong invasive phenotype reaching a maximum around 17 weeks of gestation. Read More

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http://dx.doi.org/10.1007/s12253-012-9572-2DOI Listing
April 2013
16 Reads

Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors.

J Clin Endocrinol Metab 2012 Sep 11;97(9):E1649-57. Epub 2012 Jun 11.

Institut National de la Santé et de la Recherche Médicale Unité 782, 32 Rue des Carnets, Clamart F-92140, France.

Background: Anti-müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as a useful diagnostic and prognostic tool in human reproductive endocrinology.

Objective: The aim of this study was to investigate the regulation of human ovarian AMH by estradiol and FSH. Read More

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http://dx.doi.org/10.1210/jc.2011-3133DOI Listing
September 2012
37 Reads

[CXCR4, a therapeutic target in rare immunodeficiencies?].

Med Sci (Paris) 2011 Apr 28;27(4):391-7. Epub 2011 Apr 28.

Université Paris-Sud, laboratoire cytokines, chimiokines et immunopathologie, UMR-S996, 32, rue des Carnets, 92140 Clamart, France.

Currently, more than 200 primary immunodeficiency diseases have been discovered. In most cases, genetic defects affect the expression or the function of proteins involved in immune development and homeostasis. Some orphan immuno-hematological disorders are characterized by an abnormal leukocyte trafficking, a notion predictive of an anomaly of the chemokine/chemokine receptor system. Read More

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http://dx.doi.org/10.1051/medsci/2011274015DOI Listing
April 2011
120 Reads

[WHIM syndrome: on the track of an interplay between human papillomavirus and the CXCL12 chemokine].

Med Sci (Paris) 2011 Apr 28;27(4):341-3. Epub 2011 Apr 28.

Laboratoire cytokines, chimiokines et immunopathologie, UMR-S996, Université Paris-Sud 11, 32, rue des Carnets, 92140 Clamart, France.

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http://dx.doi.org/10.1051/medsci/2011274002DOI Listing
April 2011
37 Reads

New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side.

J Reprod Immunol 2011 Mar 18;88(2):118-23. Epub 2011 Feb 18.

Unité 782 INSERM, Hôpital Antoine Béclère et Université Paris Sud Orsay, 32 rue des Carnets, 92141 Clamart Cedex, France.

Identification of biomarkers of optimal uterine receptivity to the implanting embryo as well as biomarkers of oocyte competence would undoubtedly improve the efficiency of assisted reproductive technology (ART). Expression of IL-15 and IL-18 has been shown to be different in patients with failed implantation after IVF/ICSI compared with fertile controls and both correlate with local uNK (CD56+) recruitment and angiogenesis. Tumor necrosis factor weak inducer of apoptosis (TWEAK) has been described in mice as a potent early immune regulator able to protect the conceptus. Read More

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http://dx.doi.org/10.1016/j.jri.2011.01.007DOI Listing
March 2011
12 Reads

FSH and its second messenger cAMP stimulate the transcription of human anti-Müllerian hormone in cultured granulosa cells.

Mol Endocrinol 2011 Apr 17;25(4):645-55. Epub 2011 Feb 17.

Institut National de la Santé et de la Recherche Médicale, Unité 782, 32 rue des Carnets, 92120 Clamart, France.

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance, a member of the TGF-ß family, is responsible for the regression of Müllerian ducts in the male fetus. In females, AMH is synthesized by granulosa cells of preantral and small antral follicles, and production wanes at later stages of follicle maturation. Using RT-PCR in luteal granulosa cells in primary culture and reporter gene techniques in the KK1 granulosa cell line, we show that FSH and cAMP enhance AMH transcription, and LH has an additive effect. Read More

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http://dx.doi.org/10.1210/me.2010-0297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417263PMC
April 2011
21 Reads

[Dysfunctions of the CXCL12 (SDF-1)/CXCR4 signaling axis in the WHIM syndrome and the idiopathic CD4(+) T-cell lymphocytopenia].

Biol Aujourdhui 2010 10;204(4):273-84. Epub 2011 Jan 10.

Université Paris-Sud, Laboratoire Cytonkin, Chimiokines et Immunopathologies, UMR S996, 32 rue des Carnets, 92140 Clamart, France - INSERM, 92140 Clamart, France.

Chemokines are small cytokine-like secreted proteins that govern migration of leukocytes to their specific niches in lymphoid organs and to inflammatory sites. They mediate their functions by binding to and activating chemokine receptors, which belong to the heptahelical G protein-coupled receptor family. The CXC chemokine Stromal cell Derived Factor-1 (SDF-1/CXCL12) is the sole natural ligand for the broadly expressed CXCR4 receptor and acts as a chemoattractant for many leukocyte subsets. Read More

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http://dx.doi.org/10.1051/jbio/2010022DOI Listing
April 2011
151 Reads

Treatment failure with antagonists of TNF-α: mechanisms and implications for the care of patients.

Eur Cytokine Netw 2010 Dec 18;21(4):226-31. Epub 2010 Nov 18.

INSERM U996, Service de microbiologie-Immunologie Biologique, Hôpital Antoine-Béclère, Université Paris-Sud, 32 rue des Carnets, Clamart, France.

The use of TNF-α antagonists has substantially improved the care of many patients with inflammatory and autoimmune diseases. However, approximately one third of such patients fail to respond well to treatment, regardless of the antagonist used or of the underlying disease. The mechanisms underlying these failures are analyzed in this review, and proposals made concerning how best to adapt therapeutic decisions in these instances. Read More

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http://dx.doi.org/10.1684/ecn.2010.0212DOI Listing
December 2010
49 Reads

Processing of anti-mullerian hormone regulates receptor activation by a mechanism distinct from TGF-beta.

Mol Endocrinol 2010 Nov 22;24(11):2193-206. Epub 2010 Sep 22.

Institut National de la Santé et de la Recherche Médicale U782, 32 rue des Carnets, Clamart F-92140, France.

TGF-β family ligands are translated as prepropeptide precursors and are processed into mature C-terminal dimers that signal by assembling a serine/threonine kinase receptor complex containing type I and II components. Many TGF-β ligands are secreted in a latent form that cannot bind their receptor, due to the pro-region remaining associated with the mature ligand in a noncovalent complex after proteolytic cleavage. Here we show that anti-Müllerian hormone (AMH), a TGF-β family ligand involved in reproductive development, must be cleaved to bind its type II receptor (AMHRII), but dissociation of the pro-region from the mature C-terminal dimer is not required for this initial interaction. Read More

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https://academic.oup.com/mend/article-lookup/doi/10.1210/me.
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http://dx.doi.org/10.1210/me.2010-0273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417381PMC
November 2010
18 Reads

Natural mutations of the anti-Mullerian hormone type II receptor found in persistent Mullerian duct syndrome affect ligand binding, signal transduction and cellular transport.

Hum Mol Genet 2009 Aug 20;18(16):3002-13. Epub 2009 May 20.

INSERM U782, 32 rue des Carnets, Clamart F-92140, France.

The anti-Müllerian hormone type II (AMHRII) receptor is the primary receptor for anti-Müllerian hormone (AMH), a protein produced by Sertoli cells and responsible for the regression of the Müllerian duct in males. AMHRII is a membrane protein containing an N-terminal extracellular domain (ECD) that binds AMH, a transmembrane domain, and an intracellular domain with serine/threonine kinase activity. Mutations in the AMHRII gene lead to persistent Müllerian duct syndrome in human males. Read More

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https://academic.oup.com/hmg/article-lookup/doi/10.1093/hmg/
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http://dx.doi.org/10.1093/hmg/ddp238DOI Listing
August 2009
32 Reads

Current knowledge on natural killer cells, pregnancy and pre-eclampsia. Introduction.

Authors:
Gérard Chaouat

Reprod Biomed Online 2008 Feb;16(2):170-2

U 782 INSERM, Equipe implantation et dialogue cytokinique mère conceptus, 32 rue des Carnets et Hôpital Antoine Béclère Pavillon Jean Dalsace, 92141 Clamart France.

The introduction to this review discusses briefly why immunology, perceived as difficult by assisted reproduction technology clinicians, need nevertheless be envisaged as a central actor in the reproduction process, and how the maternal immune system, initially perceived as a threat to the fetoplacental unit, is in fact utterly necessary for successful pregnancy. The key cells in such a process are uterine natural killer cells, which can act as friend or foe to the fetus, but are now known to play a key role in local vasculogenesis. As an ultimate consequence in cases of dysfunction/dysregulation, these factors result in implantation failure, abortion or pre-eclampsia. Read More

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http://dx.doi.org/10.1016/s1472-6483(10)60572-xDOI Listing
February 2008
3 Reads

Cytokines: Important for implantation?

J Assist Reprod Genet 2007 Nov 28;24(11):491-505. Epub 2007 Nov 28.

U 782 INSERM, Equipe cytokines et dialogue cytokinique mère conceptus, Université Paris Sud et Hôpîtal Antoine Béclère, 32 rue des Carnets, Clamart Cedex, France.

Problem: Cytokines are obviously very important in an established pregnancy, but what about human embryo implantation?

Methods: Literature review.

Results: We first discuss the necessity and limits of animal models, and then review the few cytokines which have been demonstrated by knock-out methods to be absolutely necessary for embryo implantation using in animal models. We then review what is known or discussed about the role of other cytokines as deduced from quantitative and/or qualitative dysregulation in animals and in humans. Read More

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http://link.springer.com/10.1007/s10815-007-9142-9
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http://dx.doi.org/10.1007/s10815-007-9142-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3455031PMC
November 2007
4 Reads

Effectors regulatory T cells in pregnancy (and autoimmunity?).

Authors:
Gérard Chaouat

Expert Rev Clin Immunol 2007 Nov;3(6):861-9

U 782 INSERM, 32 Rue des Carnets, 92141 Clamart, Cedex, France.

In this review, we put the discovery of 'suppressor T cells' in pregnancy in an historical perspective, and then highlight some of the data that precluded the resurrection of the phenomenon in the last years. We then review the evidence for the existence of Tregs in murine and human pregnancy. We then emit our opinion on some data presented in a murine abortion system. Read More

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http://dx.doi.org/10.1586/1744666X.3.6.861DOI Listing
November 2007
20 Reads

Effect of TNF antagonists on the T-lymphocyte response.

Authors:
Dominique Emilie

Joint Bone Spine 2007 Dec 20;74(6):558-9. Epub 2007 Sep 20.

INSERM U764, Paris-South University, Microbiology/Biological Immunology Department, Antoine Béclère Teaching Hospital, AP-HP, 32 rue des Carnets, 92140 Clamart, France.

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http://dx.doi.org/10.1016/j.jbspin.2007.07.003DOI Listing
December 2007
17 Reads

[Physiology of implantation].

Gynecol Obstet Fertil 2007 Sep 31;35(9):861-6. Epub 2007 Aug 31.

Inserm U 782, 32, rue des Carnets, 92141 Clamart,

This article explains why we have had to come to a central role for innate immunity rather than the threat of maternal rejection of the foetal allograft. We encompass briefly the role of inflammation in implantation, not only for invasion adhesion, but also to prepare future "tolerance". In this context, we envisage the role of TWEAK and complement. Read More

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http://dx.doi.org/10.1016/j.gyobfe.2007.07.023DOI Listing
September 2007
1 Read

The Th1/Th2 paradigm: still important in pregnancy?

Authors:
Gérard Chaouat

Semin Immunopathol 2007 Jun;29(2):95-113

Unité 782 Inserm, 32 rue des Carnets 92141 Clamart, France.

The enunciation of the T helper 1/T helper 2 (Th1/Th2) paradigm in pregnancy has represented a major step forward in our understanding of physiological and pathologic materno-foetal relationship. However, recent developments in studies of the implantation process and in the emergence of the uterine vascular bed and its control by natural killer cells and cytokines suggest that one must go beyond this hitherto useful scheme. In this review, we replace the emergence of the paradigm in its historical context and then emphasises what it does explain and what it no longer account for. Read More

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http://dx.doi.org/10.1007/s00281-007-0069-0DOI Listing
June 2007
4 Reads

Immune cells in uteroplacental tissues throughout pregnancy: a brief review.

Reprod Biomed Online 2007 Feb;14(2):256-66

U 782 INSERM, Equipe implantation et dialogue cytokinique mère conceptus, 32 rue des Carnets et Hôpital Antoine Béclère Pavillon Jean Dalsace, 92141 Clamart, France.

In a brief introduction, this review states why the presence of immune cells at the interface poses problems for an immunologist (Medawar paradigm). Different types of placentation are then discussed, and the various interactions with leukocytes, the extreme being with the equids where a certain degree of 'attack' is often seen. The limits of animal models when dealing with the human situation are emphasized. Read More

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http://dx.doi.org/10.1016/s1472-6483(10)60796-1DOI Listing
February 2007
1 Read

Immune cells in uteroplacental tissues throughout pregnancy: a brief review.

Reprod Biomed Online 2007 ;14 Spec No 1:83-93

U 782 INSERM, Equipe implantation et dialogue cytokinique mère conceptus, 32 rue des Carnets et Hôpital Antoine Béclère Pavillon Jean Dalsace, 92141 Clamart France.

In a brief introduction, this review states why the presence of immune cells at the interface poses problems for an immunologist (Medawar paradigm). Different types of placentation are then discussed, and the various interactions with leukocytes, the extreme being with the equids where a certain degree of 'attack' is often seen. The limits of animal models when dealing with the human situation are emphasized. Read More

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http://dx.doi.org/10.1016/S1472-6483(10)61462-9DOI Listing
September 2010
2 Reads

Anti-Müllerian hormone receptor defect.

Best Pract Res Clin Endocrinol Metab 2006 Dec;20(4):599-610

Unité INSERM 782, Université Paris XI, 32 rue des Carnets, 92140 Clamart, France.

Anti-Müllerian hormone (AMH), produced by gonadal somatic cells, is mainly responsible for the regression of Müllerian ducts--the anlagen of uterus and Fallopian tubes--during male sex differentiation. Like other members of the transforming growth factor beta (TGF-beta) family, AMH signals through two serine/threonine kinase receptors, of which type II is specific, and type I is shared with the bone morphogenetic protein family. Persistent Müllerian duct syndrome is a rare form of male pseudohermaphroditism characterized by the persistence of Müllerian derivatives in otherwise normally virilized males. Read More

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http://dx.doi.org/10.1016/j.beem.2006.09.004DOI Listing
December 2006
9 Reads

Type-I interferons and systemic lupus erythematosus.

Autoimmun Rev 2006 Oct 27;5(8):554-62. Epub 2006 Jun 27.

INSERM U 764 and Université Paris XI, 32 Rue des Carnets, Clamart, France.

Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease characterized by the production of autoantibodies directed against nuclear antigens and chronic inflammation affecting multiple tissues. Complex genetic disorders are at the origin of the disease in humans and in SLE-prone mice, leading to the escape of auto-reactive B-lymphocytes from central and peripheral control checkpoints that operate normally in healthy organisms. Although necessary, autoimmune B-cells are not sufficient and additional mechanisms such as T-cell help are clearly needed for the disease to occur. Read More

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http://dx.doi.org/10.1016/j.autrev.2006.05.002DOI Listing
October 2006
22 Reads

[History of spermatozoon and changing views].

Authors:
J Gonzalès

Gynecol Obstet Fertil 2006 Sep 7;34(9):819-26. Epub 2006 Sep 7.

U 782 Endocrinologie et génétique de la reproduction et du développement du Directeur-J.-Y.-Picard, 32, rue des Carnets, 92140 Clamart, France.

With Ham, Leeuwenhoek discovered animalcules in human semen in 1677, without theoretical interpretations. Discussions focused on the respective role of ovum and animalcules during two centuries with erroneous doctrines. Modern ideas on status and origin of animalcules are associated with the development of the cell theory. Read More

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http://dx.doi.org/10.1016/j.gyobfe.2006.07.025DOI Listing
September 2006
3 Reads

Inhibition of anti-tuberculosis T-lymphocyte function with tumour necrosis factor antagonists.

Arthritis Res Ther 2006 ;8(4):R114

INSERM UMR-S764, Service d'Hépato-Gastro-Entérologie, Hôpital Antoine Béclère, Assistance Publique-Hôpitaux de Paris, Institut Paris-Sud sur les Cytokines, Université Paris-Sud, INSERM U764, 32 rue des Carnets, 92140, Clamart, France.

Reactivation of latent Mycobacterium tuberculosis (Mtb) infection is a major complication of anti-tumour necrosis factor (TNF)-alpha treatment, but its mechanism is not fully understood. We evaluated the effect of the TNF antagonists infliximab (Ifx), adalimumab (Ada) and etanercept (Eta) on anti-mycobacterial immune responses in two conditions: with ex vivo studies from patients treated with TNF antagonists and with the in vitro addition of TNF antagonists to cells stimulated with mycobacterial antigens. In both cases, we analysed the response of CD4+ T lymphocytes to purified protein derivative (PPD) and to culture filtrate protein (CFP)-10, an antigen restricted to Mtb. Read More

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http://arthritis-research.biomedcentral.com/articles/10.1186
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http://dx.doi.org/10.1186/ar1994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779425PMC
February 2007
34 Reads

Cyclosporin-A inhibits ERK phosphorylation in B cells by modulating the binding of Raf protein to Bcl2.

Biochem Biophys Res Commun 2006 May;344(1):134-9

INSERM unite 131, IFR13, 32 Rue des Carnets 92140 Clamart, Université Paris XI, France.

Extracellular signal-related kinase (ERK) signaling is regulated by sequential phosphorylation of upstream kinases including Raf. We report herein that ERK phosphorylation is inhibited by a short incubation with Cyclosporin-A (CsA) in anti-IgM activated Daudi B cells. As Bcl2, through its BH4 domain, was previously shown to bind both Calcineurin (Can) and Raf proteins, we hypothesized that CsA inhibited Can binding to Bcl2 allowing the latter to bind more Raf at the mitochondria thereby diverting it from activating the ERK cascade. Read More

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http://dx.doi.org/10.1016/j.bbrc.2006.03.121DOI Listing
May 2006
14 Reads

High Mda-7 expression promotes malignant cell survival and p38 MAP kinase activation in chronic lymphocytic leukemia.

Leukemia 2006 Mar;20(3):498-504

INSERM Unité 131, IFR 13, Université Paris XI 32 Rue des Carnets, Clamart, France.

Chronic lymphocytic leukemia (CLL)-B-cells are quiescent differentiated cells that produce interleukin (IL)-10 and accumulate due to resistance to apoptosis. The mechanisms underlying such resistance are poorly understood. Herein we show that all CLL B-cells tested (30/30) display high mRNA and protein expression of the tumor suppressor Mda-7/IL-24, an IL-10 family member, in comparison to normal B cells. Read More

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http://dx.doi.org/10.1038/sj.leu.2404073DOI Listing
March 2006
1 Read

Fourth International Workshop on immunology of pre-eclampsia, December 2004, Reunion, France.

J Reprod Immunol 2005 Oct;67(1-2):103-11

INSERM U131, Unité Cytokines dans la Relation Materno-Faetale, 32 Rue des Carnets, 92141 Clamart, France.

The Fourth International Workshop on 'Reproductive Immunology, Immunological Tolerance and Immunology of Pre-eclampsia' was held in the Island of La Reunion in December 2004. Besides intense sharing of immunological data, it included also epidemiological studies from South Africa, USA and Australia, as well as genetic studies from South Africa and Mauritius, discussions on the future of graft transplant tolerance, implications of inositol-phosphoglycans in the pathogenesis of pre-eclampsia (PE), pathways to the inflammatory syndrome and other topics. Participants shared the belief that we may be witnessing significant steps forward in our comprehension of PE as an immunological event, with a prominent role for deregulation of the innate immune system, probably controlled by T cells and cytokine networks at the feto-placental interface. Read More

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http://dx.doi.org/10.1016/j.jri.2005.09.004DOI Listing
October 2005
3 Reads

The chemokine SDF-1/CXCL12 contributes to T lymphocyte recruitment in human pre-ovulatory follicles and coordinates with lymphocytes to increase granulosa cell survival and embryo quality.

Am J Reprod Immunol 2005 Nov;54(5):270-83

INSERM Unité 131 Cytokines et Immunorégulation, Institut Paris-Sud Sur les Cytokines, IFR 13, rue des Carnets, Clamart, France.

We investigated the production and the role of the chemokine stromal cell-derived factor-1 (SDF-1/CXCL12) in pre-ovulatory follicles of women undergoing in vitro fertilization. We detected CXCL12 and its receptor CXCR4 by flow cytometry, western blotting and RT-PCR. We tested cell migration in Transwell experiments. Read More

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http://dx.doi.org/10.1111/j.1600-0897.2005.00307.xDOI Listing
November 2005
83 Reads

[B lymphocytes--a dogma revisited].

Authors:
Jacques Couderc

C R Biol 2005 Aug;328(8):758-66

Inserm 131, Cytokines et Immunorégulations, 32, rue des Carnets, 92140 Clamart, France.

The one B cell-one antibody hypothesis proposed by M.F. Burnet (1957) was recently challenged by results showing that one B cell can simultaneously express an auto-reactive BCR, and a BCR directed against non-self antigens. Read More

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http://dx.doi.org/10.1016/j.crvi.2005.05.008DOI Listing
August 2005
4 Reads

[From human andro- and parthenogenesis (hydatidiform moles and benign ovarian teratomas) to cancer].

Authors:
P Coullin

Gynecol Obstet Fertil 2005 Jul-Aug;33(7-8):469-82

Inserm U 493 endocrinologie et génétique du développement et de la reproduction, 32, rue des Carnets, 92140 Clamart, France.

Genomic imprinting is a process that appeared in mammals. This phenomenon blocks the normal development of parthenogenic and androgenic conceptuses, that is to say benign ovarian teratomas and hydatidiform moles respectively. Pathological modifications of these conceptuses depend on whether the chromosomes come from the mother or father. Read More

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http://dx.doi.org/10.1016/j.gyobfe.2005.05.023DOI Listing
January 2006

Role of type I receptors for anti-Müllerian hormone in the SMAT-1 Sertoli cell line.

Oncogene 2005 Jul;24(31):4984-92

Institut National de la Santé et de la Recherche Médicale, Unité 493 sur l'Endocrinologie du Développement, Université Paris XI, 32 rue des Carnets, 92140 Clamart, France.

Anti-Müllerian hormone (AMH) is a member of the transforming growth factor-beta family responsible for regression of Müllerian ducts during male sexual differentiation and for regulation of gonadal steroidogenesis. AMH is also a gonadal tumor suppressor which mediates its effects through a specific type II receptor and the bone morphogenetic protein (BMP)-specific Smad proteins, suggesting that AMH and BMPs could also share type I receptors, namely activin-like kinases (ALKs)2, 3 or 6. However, attempts to identify a unique AMH type I receptor among them were unsuccessful. Read More

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http://dx.doi.org/10.1038/sj.onc.1208686DOI Listing
July 2005
26 Reads

IFN{alpha} enhances human B-cell chemotaxis by modulating ligand-induced chemokine receptor signaling and internalization.

Int Immunol 2005 Apr 4;17(4):459-67. Epub 2005 Mar 4.

Institut National de la Santé et de la Recherche Medicale U 131, Institut Paris-Sud sur les Cytokines, 32 rue des Carnets, 92 140, Clamart, France.

In this study, we show that IFNalpha increases the chemotaxis of human B cells to CCL20, CCL21 and CXCL12 in a dose- and time-dependent manner. The effect was maximal with 2000 IU ml(-1) IFNalpha. It peaked at 24 h and decreased thereafter. Read More

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http://dx.doi.org/10.1093/intimm/dxh227DOI Listing
April 2005
9 Reads
13 Citations
2.540 Impact Factor

Nucleosomes in the pathogenesis of systemic lupus erythematosus.

Rheum Dis Clin North Am 2004 Aug;30(3):529-58, ix

Institut des Cytokines Paris-Sud, INSERM U131, 32 Rue des Carnets, 92140 Clamart, France.

Systemic lupus erythematosus (SLE) is characterized by the development of a large array of autoantibodies that primarily are directed against the whole chromatin (antinucleosome) and its individual components, dsDNA and histones. Apoptotic defects and impaired removal of apoptotic cells could contribute to an overload of autoantigens (and in particular of nucleosomes) in circulation or in target tissues that could become available to initiate an autoimmune response. In susceptible individuals, this can lead to autoantibody-mediated tissue damage. Read More

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http://dx.doi.org/10.1016/j.rdc.2004.04.001DOI Listing
August 2004
4 Reads

Human immunodeficiency virus 1 downregulates cell surface expression of the non-classical major histocompatibility class I molecule HLA-G1.

J Gen Virol 2004 Jul;85(Pt 7):1945-1954

INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.

Human immunodeficiency virus 1 (HIV-1) downregulates cell surface expression of HLA-A and HLA-B but not HLA-C or HLA-E to ultimately escape immune defences. Here, it is shown that cell surface expression of the non-classical HLA-G1 is also downregulated by HIV-1, by using co-transfection experiments and infection with cell-free HIV-1 of HLA-G1-expressing U87 glioma cells or macrophages in primary culture. Moreover, co-transfection experiments using proviruses deleted in either nef or vpu or plasmids encoding HIV-1 Nef and Vpu mixed together with a HLA-G1-expressing construct demonstrated that HLA-G1 downregulation is Nef-independent and Vpu-dependent, contrasting with the Nef- and Vpu-dependent HLA-A2 downregulation. Read More

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http://dx.doi.org/10.1099/vir.0.79867-0DOI Listing
July 2004
15 Reads

Induction of tolerance by Porphyromonas gingivalis on APCS: a mechanism implicated in periodontal infection.

J Dent Res 2004 May;83(5):429-33

INSERM U131, Institut Paris-Sud sur les Cytokines, 32 rue des Carnets, 92140 Clamart, France.

The periodontal pathogen Porphyromonas gingivalis (Pg) is a potent inducer of the production of pro-inflammatory cytokines by neutrophils, monocytes, and macrophages, and can desensitize immune cells in vitro and in vivo. We analyzed the ability of Pg lipopolysaccharide (LPS) to induce endotoxin tolerance. Treatment of dendritic cells (DC), the human macrophage cell line THP-1, and monocytes (antigen-presenting cells, APC) with Pg. Read More

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http://dx.doi.org/10.1177/154405910408300515DOI Listing
May 2004
1 Read

Reproductive immunology 2003: reassessing the Th1/Th2 paradigm?

Immunol Lett 2004 Apr;92(3):207-14

U 131 INSERM, Hôpital Antoine Béclère, 32 rue des Carnets, 92141 Clamart, France.

We briefly review the history of concepts (some of which are still valid) which have lead to the present situation where pregnancy is viewed as being a Th2 phenomenon. We recall some of the early evidence which has been taken as supporting the general validity of this concept in murine and human pregnancy. We then recall some of the recent data dealing with "newer" cytokines and the role of uterine natural killer (NK) cells at the feto-maternal interface which fit neither with a steady-state concept nor with inflammatory cytokines, being solely "bad guys" as the paradigm would predict, nor with the concept of reduction of NK "activity" being required for successful pregnancy. Read More

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http://dx.doi.org/10.1016/j.imlet.2004.01.011DOI Listing
April 2004
14 Reads

Abnormal production of the TNF-homologue APRIL increases the proliferation of human malignant glioblastoma cell lines via a specific receptor.

Oncogene 2004 Apr;23(17):3005-12

INSERM U131, 32, rue des Carnets, 92140 Clamart, France.

A proliferation-inducing ligand (APRIL) of the tumour necrosis factor (TNF) family is produced in small amounts in many tissues and more abundantly in tumours. APRIL has been reported to promote cell growth in vivo and in vitro. It was recently shown that the production of APRIL in some glioblastoma cell lines does not lead to an increase in cell growth. Read More

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http://dx.doi.org/10.1038/sj.onc.1207350DOI Listing
April 2004
33 Reads

Anti-Müllerian hormone, beta-catenin and Müllerian duct regression.

Authors:
F Xavier S Allard

Mol Cell Endocrinol 2003 Dec;211(1-2):115-21

Unité de recherches sur l'Endocrinologie du Développement, INSERM, 32 rue des Carnets, 93140 Clamart, France.

The embryo is initially sexually indifferent, and correct sexual development is dependent on gonadal hormone production. Thus, in the male embryo, anti-Müllerian hormone (AMH), secreted by the Sertoli cells of the testis, induces regression of the Müllerian duct, the anlagen of female reproductive tract. This hormone causes ductal epithelial regression through a paracrine mechanism originating in periductal mesenchyme and the cross-talk between the mesenchymal and epithelial layers accounts for the cranial-to-caudal pattern of Müllerian regression. Read More

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http://dx.doi.org/10.1016/j.mce.2003.09.022DOI Listing
December 2003

Components of the anti-Müllerian hormone signaling pathway in gonads.

Mol Cell Endocrinol 2003 Dec;211(1-2):9-14

Unité INSERM 493, 32 rue des Carnets, Clamart 92140, France.

Anti-Müllerian hormone (AMH) is a member of the Transforming Growth Factor-beta (TGF-beta) family implicated in the regression of Müllerian ducts in male fetuses and in the development and function of gonads of both sexes. Members of the TGF-beta family signal through two types of serine/threonine kinase receptors called type I and type II, and two types of Smad proteins, receptor-regulated Smad (R-Smad) and common Smad, Smad4. Components of the AMH signaling pathway have been identified in gonads and gonadal cell lines. Read More

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http://dx.doi.org/10.1016/j.mce.2003.09.005DOI Listing
December 2003
4 Reads

[The impact of stress in the maternofetal relationship: an immunological approach].

Gynecol Obstet Fertil 2003 Sep;31(9):778-81

Inserm U131, cytokines et relation maternofoetale, 32, rue des Carnets, 92140 Clamart, France.

Pregnancy is controlled primarily, though not exclusively, by a delicate equilibrium between locally acting growth factors and cytokines, some under steroid control. The hypothesis considered here is that stress is able to influence the equilibrium between cytokines and thus lead to abortions or implantation failure. We thus detailed the studies on that topic in order to explore the psycho-neuro-immunological mechanisms concerned. Read More

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http://dx.doi.org/10.1016/s1297-9589(03)00213-3DOI Listing
September 2003
2 Reads

Implantation: can immunological parameters of implantation failure be of interest for pre-eclampsia?

J Reprod Immunol 2003 Aug;59(2):205-17

U131 INSERM, Unite Cytokines dans la Relation Materno-Fetale, 32 Rue des Carnets, 92141 Clamart, France.

We restate briefly why we consider that the Th1/Th2 paradigm, as useful as it has been, is now no longer adequate and is obsolete. We take as an example the role of IL-18, abortifacient at high doses but cardinal for the control of natural killer (NK) cell effects on spiral artery remodelling in mice, and likely also in humans. We then describe briefly our recent studies on cytokine defects and implantation failure in humans, a key feature being the link between uterine cytokine dysregulation and abnormal uterine vascular scores. Read More

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http://dx.doi.org/10.1016/s0165-0378(03)00048-2DOI Listing
August 2003
12 Reads

Transduction pathway of anti-Müllerian hormone, a sex-specific member of the TGF-beta family.

Trends Endocrinol Metab 2003 Mar;14(2):91-7

Unité de Recherches sur l'Endocrinologie du Développement (INSERM), Institut Paris-Sud sur les Cytokines, 32 rue des Carnets, 92140 Clamart, France.

Anti-Müllerian hormone (AMH), also called Müllerian inhibiting substance, is a member of the transforming growth factor beta (TGF-beta) family that represses the development and function of reproductive organs. Not for nothing did Professor Alfred Jost, who first discovered its existence, christen it 'hormone inhibitrice'! Anti-Müllerian hormone is thought to exert its effects through two membrane-bound serine/threonine kinase receptors, type 2 and type 1. Upon ligand binding, these drive receptor-specific cytoplasmic substrates, the Smad molecules, into the nucleus where they act as transcription factors. Read More

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http://dx.doi.org/10.1016/s1043-2760(03)00005-5DOI Listing
March 2003
4 Reads

[Why from clinic to biology?].

Authors:
Pierre Galanaud

J Soc Biol 2002 ;196(1)

Unité INSERM 131 (Cytokines et Immunorégulation), Institut Paris-Sud Cytokines (IFR 13), 32, rue des Carnets, 92140 Clamart.

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August 2002
2 Reads

Natural antisense RNA inhibits the expression of BCMA, a tumour necrosis factor receptor homologue.

BMC Mol Biol 2002 Apr 18;3. Epub 2002 Apr 18.

INSERM U131, Institut Paris-Sud sur les Cytokines, 32, rue des Carnets, 92140 Clamart, France.

Background: BCMA (B-cell maturation) belongs to the tumour necrosis factor receptor gene family, and is specifically expressed in mature B lymphocytes. Antisense BCMA RNA is produced by transcription from the same locus and has typical mRNA features, e.g, polyadenylation, splicing, Kozak consensus sequence and an ORF (p12). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107798PMC
http://dx.doi.org/10.1186/1471-2199-3-4DOI Listing
April 2002
13 Reads

Failure to infect embryos after virus injection in mouse zygotes.

Hum Reprod 2002 Mar;17(3):760-4

Institut National de la Santé et de la Recherche Médicale (INSERM) U-355, 32 rue des Carnets, 92140 Clamart, France.

Background: The intracytoplasmic injection of sperm raises the problem that viral elements may be transported into the oocyte by the spermatozoon or the surrounding medium. It also raises questions about how the developing zygote will behave.

Methods: We used the murine model to microinject murine cytomegalovirus (MCMV) into the zygote ooplasm and followed the changes in these microinjected zygotes in vivo and in vitro over time. Read More

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http://dx.doi.org/10.1093/humrep/17.3.760DOI Listing
March 2002
2 Reads

[Chemokines and defense-system cell homing].

J Soc Biol 2001 ;195(1):9-12

Unité INSERM 131 (Cytokines et Immunomodulation) et Institut Paris-Sud Cytokines (IFR 13), 32, rue des Carnets, 92140 Clamart.

The general properties of chemokines and their receptors are described, and the perspectives raised for cellular therapy are discussed. Specific examples are provided in the cases of the CXC chemokine SDF1 and of chemokines ligands of CCR5. Read More

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October 2001
4 Reads

Lead accumulation in the mouse ovary after treatment-induced follicular atresia.

Reprod Toxicol 2001 Jul-Aug;15(4):385-91

INSERM Unité 355, Maturation Gamétique et Fécondation 32, rue des Carnets, 92140-Clamart, France.

Although the main target of lead (Pb) toxicity is the red blood cell, Pb-associated changes in the nervous system, the kidney, and the reproductive system have also been described. The few Pb studies conducted on females revealed mostly miscarriages, premature delivery, and infant mortality in humans and animals. This study was done to correlate Pb accumulation in the ovary with damage to folliculogenesis. Read More

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http://dx.doi.org/10.1016/s0890-6238(01)00139-3DOI Listing
October 2001
1 Read