Search Our Scientific Publications & Authors

PLoS One 2018 14;13(12):e0208878. Epub 2018 Dec 14.

Department of Paediatrics, the University of Melbourne, Melbourne, Australia.

Anemia is regarded as major public health problem among adolescents in Low and Middle-Income Countries (LMICs) but there is limited primary data in many countries, including Nepal. This study investigated the prevalence and correlates of anemia in a nationally representative sample of adolescents within the 2014 National Adolescent Nutrition Survey in Nepal. A total of 3780 adolescents aged 10 to 19 years were selected from a cross-sectional survey through multi-stage cluster sampling. Read More

J Pediatr Adolesc Gynecol 2018 Dec 11. Epub 2018 Dec 11.

Institute of Maternal and Child Research, School of Medicine, University of Chile, Santiago, Chile. Electronic address:

Study Objective: The Androgen Receptor (AR) harbors a variable repeat of a variable number of glutamine residues [(CAG)n] which seems to affect inversely AR transcriptional activity. We assessed whether (CAG)n affect the sequence of the androgen-sensitive pubertal events and body composition in prepubertal girls.

Design: and participants: Nested case-control study within the Growth and Obesity Cohort Study of 1196 low-middle income children (≈50% girls) from Santiago, Chile. Read More

Invest Ophthalmol Vis Sci 2018 Dec;59(15):5965-5972

Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States.

Purpose: The purpose of this study was to investigate the effect of reducing diabetes-induced lysyl oxidase (LOX) overexpression on vascular cell apoptosis and blood-retinal barrier (BRB) characteristics in diabetic rats.

Methods: Nondiabetic rats, diabetic rats, and diabetic rats intravitreally (IV) injected with LOX siRNA or scrambled (scram) siRNA were used in the study. One month after the onset of diabetes, intravitreal injections were initiated at monthly intervals for up to three times. Read More

Retina 2018 Dec 6. Epub 2018 Dec 6.

Service d'Ophtalmologie, Hôpital Lariboisière, AP-HP, Assistance Publique-Hôpitaux de Paris, Université Paris Diderot, Sorbonne Paris Cité, France.

Purpose: To provide values of retinal vessel density (VD) in the three retinal capillary plexuses, foveal avascular zone (FAZ) area, and retinal layer thickness in a cohort of healthy subjects.

Methods: The optical coherence tomography angiography maps of 148 eyes of 84 healthy subjects, aged 22 to 76 years, were analyzed for measuring VD of the retinal capillary plexuses, using the Optovue device comprising a projection artifact removal algorithm. Foveal avascular zone metrics were measured, and the relationship between optical coherence tomography angiography findings and age, sex, and image quality was studied. Read More

J Cell Mol Med 2018 Dec 13. Epub 2018 Dec 13.

Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

The poor survival of stem cells seriously limits their therapeutic efficacy for myocardial infarction (MI). Mineralocorticoid receptor (MR) activation plays an important role in the pathogenesis of multiple cardiovascular diseases. Here, we examined whether MR silencing in bone marrow derived mesenchymal stem cells (MSCs) could improve MSCs' survival and enhance their cardioprotective effects in MI. Read More

J Cell Physiol 2018 Dec 13. Epub 2018 Dec 13.

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Microsatellite instability (MSI) is a prognostic marker in colorectal cancer (CRC). The biological significance of MSI-low (MSI-L) phenotype and its differences with microsatellite stable (MSS) phenotype remains unclear. The aim of this study is indicating the role of mononucleotide repeat in identifying MSI-L and revealing the association of MSI-L with elevated microsatellite alterations at selected tetranucleotide repeats (EMAST) and oncologic outcome in CRC patients. Read More

Small 2018 Dec 13:e1804549. Epub 2018 Dec 13.

Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, Guangdong, 518060, China.

Understanding the mechanical stability of granular-armored liquid marbles is prerequisite for their applications including encapsulation, sensors, microreactions, and miniaturized liquid storage. Most liquid marbles are armored with agglomerated granular structure which complicates the wetting and interacting states of particles, hence, impeding one from understanding the effect of granular size on the mechanical stability of marbles. In this work, using a custom-built platform to examine the liquid marbles armored by a single layer of uniform grains, it is revealed that larger microsized grains produce stronger liquid marble. Read More

Proteomics Clin Appl 2018 Dec 13:e1800135. Epub 2018 Dec 13.

A. K. Lucas-Herald, B. Mansoorian, W. Mullen, C. Delles, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, G12 8TA, UK.

Background: Progeria-like syndromes offer a unique insight into ageing. Here we present the case of a boy affected with mandibuloacral dysplasia and compound heterozygous mutations in ZMPSTE24.

Methods: Capillary electrophoresis-mass spectroscopy was used for proteome analysis to analyse peptides previously found to be differentially regulated in chronic kidney disease (273 peptides defining the CKD273 classifier), coronary artery disease (238 peptides defining the CAD238 classifier) and ageing (116 peptides defining the AGE116 classifier). Read More

Electrophoresis 2018 Dec 13. Epub 2018 Dec 13.

BIOS-Lab on a Chip Group, MESA+ Institute of Nanotechnology, TechMed Centre and Max Planck Center for Complex Fluid Dynamics, University of Twente, Enschede, Overijssel, The Netherlands.

Point-of-care systems based on microchip capillary electrophoresis require single-use, disposable microchips pre-filled with all necessary solutions so an untrained operator only needs to apply the sample and perform the analysis. While microchip fabrication can be (and has been) standardized, some manufacturing differences between microchips are unavoidable. To improve analyte precision without increasing device costs or introducing additional error sources, we recently proposed the use of integrated internal standards (ISTDs): ions added to the BGE in small concentrations which form system peaks in the electropherogram that can be used as a measurement reference. Read More

Electrophoresis 2018 Dec 13. Epub 2018 Dec 13.

Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, SC, 29634.

Extracellular vesicles, including microvesicles and exosomes, are lipidic membrane-derived vesicles that are secreted by most cell types. Exosomes, one class of these vesicles that are 30-100 nm in diameter, hold a great deal of promise in disease diagnostics, as they display the same protein biomarkers as their originating cell. For exosomes to become useful in disease diagnostics, and as burgeoning drug delivery platforms, they must be isolated efficiently and effectively without compromising their structure. Read More

Electrophoresis 2018 Dec 13. Epub 2018 Dec 13.

School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.

Despite the numerous techniques developed for the studying nanoparticle and peptide interaction nowadays, sensitive and convenient assay in the process of flow, especially to simulate the self-assembly of quantum dots and peptide in-flow in blood vessels, still remains big challenges. Here we report a novel assay for studying the self-assembly of quantum dots and peptide, based on capillary electrophoresis using a bending capillary. We demonstrate that the semicircles numbers of the bending capillary affect the self-assembly kinetics of CdSe/ZnS quantum dots and ATTO-D3LVPRGSGP G H peptide (ATTO-LVP). Read More

Electrophoresis 2018 Dec 13. Epub 2018 Dec 13.

Faculty of Science, Department of Physical and Macromolecular Chemistry, Charles University in Prague, Prague, Czech Republic.

The linear theory of electromigration, including the first-order nonlinear approximation, is generalized to systems with any equilibria fast enough to be considered instantaneous in comparison with the time-scale of peak movement. For example, this theory is practically applied in the electrokinetic chromatography (EKC) mode of the capillary zone electrophoresis. The model enables the calculation of positions and shapes of analyte and system peaks without restricting the number of selectors, the complexation stoichiometry, or simultaneous acid-base equilibria. Read More

Electrophoresis 2018 Dec 12. Epub 2018 Dec 12.

College of Chemistry, Fuzhou University, Fuzhou, 350108, P. R. China.

An organic-silica hybrid monolith was prepared by a single-step ring-opening polymerization of octaglycidyldimethylsilyl polyhedral oligomeric silsesquioxane (POSS-epoxy), poly(ethylenimine) (PEI) and β-cyclodextrin (β-CD) in a ternary porogenic solvent consisting of poly(ethylene glycol) (PEG), 1,4-butanediol and 1-propanol. The framework of POSS-PEI hybrid monolith could offer well-defined 3D skeleton, while β-CD with the ability of forming a host-guest inclusion complexes with a variety of compounds could show an ability of specific selection. The obtained hybrid monoliths were successfully applied for separation of phenols, benzoic acids and nucleobases. Read More

Prenat Diagn 2018 Dec 12. Epub 2018 Dec 12.

Department of Pediatrics, Hospital for Sick Children, Toronto, ON.

Objective: To assess the cerebral and vascular development in fetuses with hypoplastic left heart syndrome (HLHS).

Methods: Pregnant women carrying fetuses diagnosed with HLHS who decided to interrupt their pregnancies were included in our study. Aortic size and blood flow were assessed based from fetal echocardiography. Read More

J Diabetes Sci Technol 2018 Dec 14:1932296818821105. Epub 2018 Dec 14.

1 Institut für Diabetes-Technologie, Forschungs- und Entwicklungsgesellschaft mbH an der Universität Ulm, Ulm, Germany.

Background:: Analytical quality of blood glucose monitoring systems (BGMS) is an important aspect for many diabetes patients. Sufficiently high analytical quality is required for adequate diabetes therapy.

Methods:: In this study, system accuracy and measurement precision of a BGMS were assessed based on ISO 15197:2013. Read More

J Cardiol Cases 2013 Jul 6;8(1):e13-e16. Epub 2013 Apr 6.

Department of Medicine and Biological Science, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511, Japan.

We report a case of a 41-year-old male with end-stage renal disease receiving chronic hemodialysis who was referred to this hospital because of dyspnea. He had been on a regular dialysis for 20 years due to chronic glomerulonephritis. His transthoracic echocardiography revealed severe pulmonary hypertension (PH), and cardiac catheterization confirmed this diagnosis. Read More

Methods Mol Biol 2019 ;1894:281-299

Pharmaceutical Sciences Department, University of Kentucky Academic Medical Center, Lexington, KY, USA.

The role and functional anatomy of the blood-brain barrier (BBB) is summarized to enable the investigator to appropriately address evaluation of nanomaterial interaction with, and distribution across, it into brain tissue (parenchyma). Transport mechanisms across the BBB are presented, in relation to nanomaterial physicochemical properties. Measures and test substances to assess BBB integrity/disruption/permeation are introduced, along with how they are used to interpret the results obtained with the presented methods. Read More

J Cancer Res Clin Oncol 2018 Dec 13. Epub 2018 Dec 13.

UMR Inserm 1066, CNRS 6021, Université d'Angers, CHU, IBS, IRIS, MINT, UNIV Angers, INSERM, CNRS, Université Bretagne Loire, IBS-CHU, 4, rue Larrey, 49933, Angers, France.

Tumour angiogenesis is defined by an anarchic vasculature and irregularities in alignment of endothelial cells. These structural abnormalities could explain the variability in distribution of nanomedicines in various tumour models. Then, the main goal of this study was to compare and to characterize the tumour vascular structure in different mouse models of melanoma tumours (B16F10 and SK-Mel-28) and in human melanomas from different patients. Read More

Oncoimmunology 2019 22;8(1):e1486949. Epub 2018 Oct 22.

Department of Biomedical Sciences and Human Oncology, Unit of Internal Medicine "Guido Baccelli", University of Bari Medical School, Bari, Italy.

Endothelial cells (EC) line the bone marrow microvasculature and are in close contact with CD8 T cells that come and go across the permeable capillaries. Because of these intimate interactions, we investigated the capacity of EC to act as antigen-presenting cells (APC) and modulate CD8 T cell activation and proliferation in bone marrow of patients with multiple myeloma (MM) and monoclonal gammopathy of undetermined significance. We found that EC from MM patients show a phenotype of semi-professional APC given that they express low levels of the co-stimulatory molecules CD40, CD80 and CD86, and of the inducible co-stimulator ligand (ICOSL). Read More

Radiol Case Rep 2019 Mar 4;14(3):304-308. Epub 2018 Dec 4.

Department of Diagnostic and Interventional Radiology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.

Choroid plexus papillomas (CPPs) are rare neoplasms classified as World Health Organization grade I tumors. CPPs containing other tissues have occasionally been documented in the literature. However, few of these previous reports have provided clinical and radiological information. Read More

Exp Ther Med 2018 Dec 15;16(6):5021-5024. Epub 2018 Oct 15.

Pneumology Department, The Affiliated Traditional Medical Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.

The aim of this study was to investigated the functional changes of airway epithelial cells and mitochondria in rat models of asthenic lung and phlegm blocking combined with cough variant asthma (CVA). Sixteen Sprague-Dawley rats were randomly divided into two groups: Control and model group, with 8 rats in each group. On the basis of the CVA rat model induced and sensitized by ovalbumin and aluminum hydroxide, the rat models with asthenic lung and phlegm blocking combined with CVA were established via smoking stimulation. Read More

Front Immunol 2018 28;9:2774. Epub 2018 Nov 28.

Simon Lab, Department of Biomedical Engineering, University of California, Davis, Davis, CA, United States.

Neutrophils are the most motile of mammalian cells, a feature that enables them to protect the host against the rapid spread of pathogens from tissue into the circulatory system. A critical process is the recruitment of neutrophils to inflamed endothelium within post-capillary venules. This occurs through cooperation between at least four families of adhesion molecules and G-protein coupled signaling receptors. Read More

NPJ Schizophr 2018 Dec 13;4(1):26. Epub 2018 Dec 13.

Mental Health Research Centre, Zagorodnoe shosse 2, 117152, Moscow, Russia.

Microglial activation has been proposed to be involved in the pathophysiology of schizophrenia (SCZ). We hypothesized that dystrophic alterations of oligodendrocytes previously reported in the prefrontal white matter in SCZ might be associated with microglial activation in the acute state of SCZ. White matter of the prefrontal cortex (BA10) was studied in post-mortem brain tissue from 21 SCZ cases and 20 normal controls. Read More

Eur Respir J 2018 Dec 13. Epub 2018 Dec 13.

Cardiovascular Institute, Dept of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA.

Clinical and translational research has played a major role in advancing our understanding of pulmonary hypertension (PH), including pulmonary arterial hypertension and other forms of PH with severe vascular remodelling ( chronic thromboembolic PH and pulmonary veno-occlusive disease). However, PH remains an incurable condition with a high mortality rate, underscoring the need for a better transfer of novel scientific knowledge into healthcare interventions. Herein, we review recent findings in pathology (with the questioning of the strict morphological categorisation of various forms of PH into pre- or post-capillary involvement of pulmonary vessels) and cellular mechanisms contributing to the onset and progression of pulmonary vascular remodelling associated with various forms of PH. Read More

Eur Respir J 2018 Dec 13. Epub 2018 Dec 13.

Pulmonary Circulation Unit, Pulmonary Division, Heart Institute (InCor), Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil.

Since the 1st World Symposium on Pulmonary Hypertension (WSPH) in 1973, pulmonary hypertension (PH) has been arbitrarily defined as mean pulmonary arterial pressure (mPAP) ≥25 mmHg at rest, measured by right heart catheterisation. Recent data from normal subjects has shown that normal mPAP was 14.0±3. Read More

EBioMedicine 2018 Dec 10. Epub 2018 Dec 10.

Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China.. Electronic address:

Background: We previously found that loss of lncRNA-AZIN2 splice variant (AZIN2-sv) increases cardiomyocyte (CM) proliferation and attenuates adverse ventricular remodelling post-myocardial infarction (MI). However, whether inhibition of AZIN2-sv can simultaneously induce angiogenesis and thus improve prognosis after MI is unclear.

Methods: We used in situ hybridization and quantitative PCR to determine AZIN2-sv expression in endothelial cells. Read More

Prim Care Diabetes 2018 Dec 10. Epub 2018 Dec 10.

Epidemiology and Biostatistics, School of Population Health, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address:

Aim: To describe quality management processes and appropriate interpretation with respect to HbA point-of-care (POC) testing in a national diabetes and cardiovascular risk screening programme.

Methods: We compared HbA results from capillary blood, measured by the cobas b 101 (Roche Diagnostics) POC testing system, with results from venous blood measured by accredited laboratory analysers to inform national screening practice and a (separately-reported) randomised controlled trial. Difference plots and regressions were used to aid interpretation around 40 and 50mmol/mol, the cut-offs used to identify "pre-diabetes" and diabetes in New Zealand. Read More

Biochem Biophys Res Commun 2018 Dec 10. Epub 2018 Dec 10.

Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul, South Korea; Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, South Korea. Electronic address:

Renal fibrosis is the final common pathway of various renal injuries and it leads to chronic kidney disease. Recent studies reported that FOXD1-lineage pericyte plays a critical role in tubulointerstitial fibrosis (TIF). However the regulatory mechanisms remain unclear. Read More

Nutrients 2018 Dec 7;10(12). Epub 2018 Dec 7.

Research Centre for Musculoskeletal Science & Sports Medicine, Department of Exercise and Sport Science, Manchester Metropolitan University Cheshire, Crewe CW1 5DU, UK.

The aim of this study was to determine the response to an oral glucose tolerance test (OGTT) in adult males with Becker muscular dystrophy (BMD) and Duchenne muscular dystrophy (DMD), and to investigate whether body composition contributes to any variance in the glucose response. Twenty-eight adult males with dystrophinopathy (BMD, = 13; DMD, = 15) and 12 non-dystrophic controls, ingested 75 g oral anhydrous glucose solution. Fingertip capillary samples were assessed for glucose at 30-min intervals over 2-h post glucose ingestion. Read More

Medicine (Baltimore) 2018 Dec;97(49):e13279

Department of Endocrinology, The Second Affiliated Hospital of Zhejiang University School of Medicine.

Rationale: Hyperpigmentation is a common skin disease. However, there are few reported cases of Grave's disease with diffuse hyperpigmentation. We hereby described a rare case with diffuse hyperpigmentation induced by Grave's disease. Read More

J Neurosurg 2018 Dec 1:1-7. Epub 2018 Dec 1.

The authors report on a 57-year-old woman in whom progression to brain death occurred on day 9 after aneurysmal subarachnoid hemorrhage without evidence of significant brain edema or vasospasm. Neuromonitoring demonstrated that brain death was preceded by a series of cortical spreading depolarizations that occurred in association with progressive hypoxic episodes. The depolarizations induced final electrical silence in the cortex and ended with a terminal depolarization that persisted > 7 hours. Read More

Cardiorenal Med 2018 Dec 13;9(2):83-91. Epub 2018 Dec 13.

Department of Medicine, Einstein Medical Center, Philadelphia, Pennsylvania, USA.

Background: Renal dysfunction is an important predictor of poor outcomes in patients with heart failure with preserved ejection fraction (HFpEF). Right ventricular (RV) dysfunction is implicated as one of the explanations for worsening renal function in cardiorenal syndrome. Novel right heart catheterization (RHC) parameters such as pulmonary artery pulsatility index (PAPi) and right atrial to pulmonary capillary wedge pressure ratio (RA:PCWP) have been found as predictors of RV dysfunction. Read More

Forensic Sci Int Genet 2018 Nov 28;39:44-49. Epub 2018 Nov 28.

Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Linköping, Sweden; Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.

The recent year's development of massively parallel sequencing (MPS) instruments and assays have now made it a compatible complement to the established capillary electrophoresis (CE) analysis for different forensic genetic applications. It is well known that short tandem repeat (STR) alleles of the same fragment size could have different DNA sequences. Thus, there will be an expected increase in the population genetic diversity for the present set of forensic STRs when performing the analysis with MPS technologies and taking the internal DNA sequence into account. Read More

Microvasc Res 2018 Dec 10. Epub 2018 Dec 10.

Department of Sciences and Humanities, National Institute of Technology, Uttarakhand-246174, India. Electronic address:

The flow through curved microvessels has more realistic applications in physiological transport phenomena especially in blood flow through capillary and microvessels. Motivated by the biomicrofluidics applications, a mathematical model is developed to describe the blood flow inside a curved microvessel driven by electroosmosis. In addition to this flow, the channel experiences electric double layer phenomenon due to zeta potential about -25mV. Read More

ACS Nano 2018 Dec 13. Epub 2018 Dec 13.

Wearable systems could offer noninvasive and real-time solutions for monitoring of biomarkers in human sweat as an alternative to blood testing. Recent studies have demonstrated that the concentration of certain biomarkers in sweat, can be directly correlated to their concentrations in blood, making sweat a trusted biofluid candidate for non-invasive diagnostics. We introduce a wearable sweat sensing system to track biochemical information at the surface of the skin in real time. Read More

Soft Matter 2018 Dec 13. Epub 2018 Dec 13.

Surface du Verre et Interfaces, UMR 125 CNRS/Saint-Gobain, 93300 Aubervilliers, France and Department of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA.

Withdrawing a plate from a suspension leads to the entrainment of a coating layer of fluid and particles on the solid surface. In this article, we study the Landau-Levich problem in the case of a suspension of non-Brownian particles at moderate volume fraction 10% < φ < 41%. We observe different regimes depending on the withdrawal velocity U, the volume fraction of the suspension φ, and the diameter of the particles 2a. Read More

Pol Arch Intern Med 2018 Dec 13. Epub 2018 Dec 13.

INTRODUCTION Raynaud Phenomenon is a medical condition in which spasm of arteries cause episodes of reduced blood flow. Potential disorders in the microcirculation R2.1a of the oral mucosa may promote the occurrence of lesions. Read More

Ann Biol Clin (Paris) 2018 Dec;76(6):675-680

Département de biochimie et génétique, CHU Angers, France.

We report the case of an infant hospitalized for neonatal anoxic ischemia in whom the diagnosis of Alagille syndrome (SAG ; MIM # 118450) was suspected in the presence of major cholestasis, cardiac malformations, suggestive facial dysmorphia, and vertebral and ocular abnormalities. This diagnosis was later confirmed by the detection of a heterozygous pathogenic variant in the gene JAG1, i.e. Read More

Folia Morphol (Warsz) 2018 Dec 13. Epub 2018 Dec 13.

Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.

This study aimed to investigate the antioxidative and anti-inflammatory effects of caffeic acid phenethyl ester (CAPE) on damage caused to cerebellum tissue by diffuse traumatic head trauma via biochemical, histopathologic, and immuno-histochemical methods. Male Sprague-Dawley (300-350g) rats were subjected to traumatic brain injury with a weight-drop device (300 g-1 m weight-height impact). Twenty-four adult rats were randomly divided into three equal groups of 8, including a control group, traumatic brain injury (TBI) group, and TBI+CAPE treatment group (10μmol/kg/i. Read More

Eur J Appl Physiol 2018 Dec 12. Epub 2018 Dec 12.

Post-graduate Program in Translational Medicine, Federal University of São Paulo, São Paulo, SP, Brazil.

Purpose: High cardiac vagal control in endurance athletes has been generally associated with adequate recovery from training and readiness to cope high-intensity training. A method that improves cardiac vagal control in endurance athletes could therefore be advantageous. Accordingly, we sought to test whether ischemic preconditioning (IPC) could enhance cardiac vagal control in endurance runners. Read More

Environ Sci Process Impacts 2018 Dec 13. Epub 2018 Dec 13.

Soil, Water, and Environmental Science Department, School of Earth and Environmental Sciences, The University of Arizona, Tucson, AZ 85721, USA. and Hydrology and Atmospheric Sciences Department, School of Earth and Environmental Sciences, The University of Arizona, Tucson, AZ 85721, USA.

Pore-scale fluid processes in geological media are critical for numerous applications in several fields. Continued improvement of high-resolution image acquisition and processing methods has provided a means to directly characterize pore-scale fluid processes for natural geomedia, and to test the representativeness of theoretical and computational models developed to simulate them. High-resolution synchrotron X-ray microtomography (XMT) combined with advanced 3-D image visualization was used to investigate the impact of larger-scale solid-surface heterogeneity on nonwetting-wetting (air-water) interfacial area for two natural geomedia (a sand and a soil). Read More

Analyst 2018 Dec 13. Epub 2018 Dec 13.

Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.

In situ capillary microsampling with capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) enabled the characterization of cationic metabolites in single cells in complex tissues and organisms. For deeper coverage of the metabolome and metabolic networks, analytical approaches are needed that provide complementary detection for anionic metabolites, ideally using the same instrumentation. Described here is one such approach that enables sequential cationic and anionic (dual) analysis of metabolites in the same identified cell in a live vertebrate embryo. Read More

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2018 Nov;30(11):1071-1076

Department of Critical Care Medicine, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning, China. Corresponding author: Zang Bin, Email:

Objective: To investigate the regulative effect of hemichannels protein Pannexin-1 on P2X7 receptor activation and caspase-1 mediated inflammatory response in the lungs of mice with lung injury.

Methods: Sixty male C57BL/6 mice were randomly divided into five groups with 12 mice in each group: sham operation group (Sham group), mechanical ventilation (MV) group, MV + low dose lipopolysaccharide (LPS) group (MLL group), MV + medium and high dose LPS group (MML group) and MV + high dose LPS group (MHL group). A "two-hit" lung injury model was reproduced by MV with high tidal volume combined with LPS injection in airway. Read More

MAbs 2018 Dec 12:1-19. Epub 2018 Dec 12.

a UltraHuman Limited, Codebase , Edinburgh , UK.

Monoclonal anti-programmed cell death 1 (PD1) antibodies are successful cancer therapeutics, but it is not well understood why individual antibodies should have idiosyncratic side-effects. As the humanized antibody SHR-1210 causes capillary hemangioma in patients, a unique toxicity amongst anti-PD1 antibodies, we performed human receptor proteome screening to identify nonspecific interactions that might drive angiogenesis. This screen identified that SHR-1210 mediated aberrant, but highly selective, low affinity binding to human receptors such as vascular endothelial growth factor receptor 2 (VEGFR2), frizzled class receptor 5 and UL16 binding protein 2 (ULBP2). Read More

J Cell Biochem 2018 Dec 7. Epub 2018 Dec 7.

Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, China.

Current evidence supports the use of bone marrow-derived mesenchymal stem cells (MSCs) for a diverse range of clinical applications, and many studies have shown that MSCs have renal-protective effects, but the mechanism is not well understood. Therefore, in this study, we aim to further identify whether MSCs can attenuate renal fibrosis by decreasing tubulointerstitial injury in a unilateral ureteral obstruction (UUO) model. In this study, we cultured MSCs and then transplanted them into a UUO model through the tail vein. Read More

Regen Ther 2018 Dec 19;9:79-88. Epub 2018 Sep 19.

Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science, 86 Nishi-cho, Yonago 683-8503, Japan.

Introduction: Cell sheets using myoblasts have been developed for the treatment of heart failure after myocardial infarction (MI) bridging to heart transplantation. Stem cells are supposed to be better than myoblasts as a source of cells, since they possess a potential to proliferate and differentiate into cardiomyocytes, and also have capacity to secrete angiogenic factors. Adipose-derived stem cells (ASCs) obtained from fat tissues are expected to be a new cell source for ASC sheet therapies. Read More

Regen Ther 2019 Jun 20;10:1-9. Epub 2018 Nov 20.

Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata City, Osaka, 573-1010, Japan.

Introduction: Human platelet lysate (hPL) part of the growth factor cocktail derived from human platelets, which has been applied as a cell growth supplement. The production process is easier in comparison to platelet-rich plasma; thus, hPL is now considered for use in wound healing therapy. However, methods for preserving hPL for more than several months that maintain its bioactivity must be considered, especially for chronic wound treatment. Read More

Nanotechnology 2018 Nov 26. Epub 2018 Nov 26.

Department of Mechanical Engineering, Texas Tech University, Box 41021, Lubbock, TX 79409-1021, USA, Lubbock, UNITED STATES.

Lithography-free nanomanufacturing by elongation and fracture of glass forming metallic liquid is presented. The viscous metallic liquid confined in a cavity is laterally downsized to nanoscale by stretching. The extent of size-reduction can be controlled by tuning the active volume of liquid and the viscous and capillary stresses. Read More

Chemphyschem 2018 Dec 6. Epub 2018 Dec 6.

RUSSIAN FEDERATION.

Previously unknown transformation of aldehydes, ketones and carboxylic acid derivatives leads to the formation of substituted oxiranes, aziridines and azirines as shown by DFT and MP2 computations. Formations of 2,2-dimethyloxirane-d8 from acetone-d6, phenylazirine-d2 from benzonitrile and 2-methyl-2-(4-hydroxyphenyl)-oxirane from 4-hydroxyacetophenone were detected experimentally by electrospray ionization mass-spectrometry with heated desolvating capillary. This reaction is a truly concerted process characterized by high activation barriers (activation enthalpies 320-480 kJ/mol). Read More

Syst Parasitol 2018 Dec 6. Epub 2018 Dec 6.

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Petrozavodsk, Russia.

We isolated and re-investigated Nephrocystidium pickii Weißenberg, 1921 (Myxozoa: Bivalvulida: Variisporina) using light microscopy and phylogenetic analysis of the small-subunit (SSU) ribosomal RNA gene. This species is a parasite of the northern pike Esox lucius L. (Actinopterygii: Esocidae) which localizes in the endothelial cells of the glomerular capillary. Read More