968,032 results match your criteria Cancer cell & microenvironment[Journal]


Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population.

Clin Exp Dent Res 2020 Jul 11. Epub 2020 Jul 11.

Oral and Maxillofacial Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Background: Undifferentiated pleomorphic sarcomas are one of the most common subtypes of soft tissue sarcomas. These are aggressive mesenchymal tumors and are devoid of the major known biomarkers except vimentin. Our objective was to establish and characterize a primary cell population from a mandibular UPS specimen. Read More

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http://dx.doi.org/10.1002/cre2.301DOI Listing

LINC00160 mediated paclitaxel-And doxorubicin-resistance in breast cancer cells by regulating TFF3 via transcription factor C/EBPβ.

J Cell Mol Med 2020 Jul 11. Epub 2020 Jul 11.

Center for Precision Medicine, Anhui No.2 Provincial People's Hospital, Hefei, China.

Chemoresistance represents a major challenge in breast cancer (BC) treatment. This study aimed to probe the roles of LINC00160 in paclitaxel- and doxorubicin-resistant BC cells. Three pairs of BC and adjacent normal tissue were used for lncRNA microarray analysis. Read More

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http://dx.doi.org/10.1111/jcmm.15487DOI Listing

Knockdown of DNA binding protein A (dbpA) enhances the chemotherapy sensitivity of colorectal cancer via suppressing the Wnt/β-catenin/Chk1 pathway.

Cell Biol Int 2020 Jul 11. Epub 2020 Jul 11.

Department of hepatobiliary surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P.R. China.

DNA-binding protein A (dbpA) is reported to be upregulated in many cancers and associated with tumor progress. The present study aimed to investigate the role of dbpA in 5-Fluorouracil (5-FU)-resistant and Oxaliplatin (L-OHP)-resistant colorectal cancer (CRC) cells. We found that 5-FU and L-OPH treatment promoted the expression of dbpA. Read More

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http://dx.doi.org/10.1002/cbin.11416DOI Listing

The impact of antibiotic allergy testing in transplant patients.

Transpl Infect Dis 2020 Jul 11:e13411. Epub 2020 Jul 11.

Department of Infectious Diseases and Centre for Antibiotic Allergy and Research, Austin Health,, VIC, Australia.

Patient reported antibiotic allergies, so-called antibiotic allergy labels (AAL) are highly prevalent amongst hospitalized patients globally . The highest prevalence of AALs has been found to be in immunocompromised patients, in particular transplant patients, with a large U.S. Read More

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http://dx.doi.org/10.1111/tid.13411DOI Listing

Potentiation of calcium-activated chloride secretion and barrier dysfunction may underlie EGF receptor tyrosine kinase inhibitor-induced diarrhea.

Physiol Rep 2020 Jul;8(13):e14490

Division of Gastroenterology, Department of Medicine, University of California San Diego, La Jolla, CA, USA.

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFr TKIs) are first-line therapies for various cancers, and cause dose-limiting severe diarrhea in many patients. We hypothesized that diarrhea caused by EGFr TKIs might reflect actions on epithelial transport, barrier function, or both, which we tested using cell cultures including murine and human enteroid-derived monolayers (EDMs), analyzed using electrophysiological and other relevant methods. EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Read More

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http://dx.doi.org/10.14814/phy2.14490DOI Listing

Increased Inflammasome Activity in Subcutaneous Adipose Tissue of Patients with Metabolic Syndrome.

Diabetes Metab Res Rev 2020 Jul 11. Epub 2020 Jul 11.

VA Medical Center, Mather, California.

Aims: The metabolic syndrome (MetS) is an inflammatory disorder associated with an increased risk for diabetes and atherosclerotic cardiovascular disease (ASCVD). Studies in patients and animal models of obesity and diabetes have shown increased NOD-like receptor family pyrin domain containing 3 (NLPR3) inflammasome activity. However, there is scanty data on the activity of the NLRP3 inflammasome in patients with nascent MetS. Read More

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http://dx.doi.org/10.1002/dmrr.3383DOI Listing

MIB1-mediated degradation of WRN promotes cellular senescence in response to camptothecin treatment.

FASEB J 2020 Jul 11. Epub 2020 Jul 11.

Department of Medical Genetics, Center for Medical Genetics, Peking University Health Science Center, Beijing, China.

Werner syndrome protein (WRN) plays critical roles in DNA replication, recombination, and repair, as well as transcription and cellular senescence. Ubiquitination and degradation of WRN have been reported, however, the E3 ubiquitin ligase of WRN is little known. Here, we identify mindbomb E3 ubiquitin protein ligase 1 (MIB1) as a novel E3 ubiquitin ligase for WRN protein. Read More

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http://dx.doi.org/10.1096/fj.202000268RRRDOI Listing

DNMT3B deficiency alters mitochondrial biogenesis and α-ketoglutarate levels in human embryonic stem cells.

Stem Cells 2020 Jul 11. Epub 2020 Jul 11.

Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo and Oslo University Hospital, Oslo, Norway.

Embryonic stem cell (ESC) renewal and differentiation is regulated by metabolites that serve as co-factors for epigenetic enzymes. Increase of α-ketoglutarate (α-KG), a co-factor for histone and DNA demethylases, triggers multi-lineage differentiation in human ESCs. To gain further insight how the metabolic fluxes in pluripotent stem cells can be influenced by inactivating mutations in epigenetic enzymes, we generated human ESCs deficient for de novo DNA methyltransferases (DNMT) 3A and 3B. Read More

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http://dx.doi.org/10.1002/stem.3256DOI Listing

Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A).

Hum Mutat 2020 Jul 11. Epub 2020 Jul 11.

Brain and Mitochondrial Research Group, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia.

Defects in the motor domain of kinesin family member 1A (KIF1A), a neuron-specific ATP-dependent anterograde axonal transporter of synaptic cargo, are well-recognized to cause a spectrum of neurological conditions, commonly known as KIF1A-associated neurological disorders (KAND). Here we report one mutation-negative female with classic Rett syndrome (RTT) harboring a de novo heterozygous novel variant [NP_001230937.1:p. Read More

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http://dx.doi.org/10.1002/humu.24079DOI Listing

Epithelial/mesenchymal heterogeneity of high-grade serous ovarian carcinoma samples correlates with miRNA let-7 levels and predicts tumor growth and metastasis.

Mol Oncol 2020 Jul 11. Epub 2020 Jul 11.

Department of Basic Sciences, Division of Biochemistry, Loma Linda University, Loma Linda, CA, USA.

Patient-derived samples present an advantage over current cell line models of high grade serous ovarian cancer (HGSOC) that are not always reliable and phenotypically faithful models of in vivo HGSOC. To improve upon cell line models of HGSOC, we set out to characterize a panel of patient-derived cells and determine their epithelial and mesenchymal characteristics. We analyzed RNA and protein expression levels in patient-derived xenograft (PDX) models of HGSOC, and functionally characterized these models using flow cytometry, wound healing assays, invasion assays and spheroid cultures. Read More

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http://dx.doi.org/10.1002/1878-0261.12762DOI Listing

Clinical characteristics and outcome of SARS-CoV-2 infection in Italian pediatric oncology patients: a study from the Infectious Diseases Working Group of the AIEOP.

J Pediatric Infect Dis Soc 2020 Jul 11. Epub 2020 Jul 11.

Pediatric Hematology Oncology, Department of Mother and Child, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy.

Background: Little is known as yet about the outcome of SARS-CoV-2 infection in children being treated for cancer.

Methods: We collected information on the clinical characteristics and outcomes of a cohort of 29 children (16 females and 13 males, median age 7 years, range [0-16]) diagnosed with SARS-CoV-2 infection while on chemotherapy/immunotherapy (N=26), or after stem cell transplantation (N=3) during the peak of the epidemic in Italy. These patients suffered from leukemia (N=16), lymphoma (N=3), solid tumors (N=10), and Langerhans cell histiocytosis (N=1). Read More

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http://dx.doi.org/10.1093/jpids/piaa088DOI Listing

The circular RNA circEIF3M promotes breast cancer progression by promoting cyclin D1 expression.

Aging (Albany NY) 2020 Jul 11;12. Epub 2020 Jul 11.

Department of General Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

We investigated the function of circular RNA circEIF3M (hsa_circ_0003119) in triple-negative breast cancer. The expression profiles of circRNAs in 3 specimens of triple-negative breast cancer tissues with adjacent nontumor tissues were analyzed by RNA-sequencing. We verified the oncogenic role of circEIF3M in triple-negative breast cancer through a series of biological function experiments. Read More

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http://dx.doi.org/10.18632/aging.103539DOI Listing

A model based on tumor-infiltrating immune cells for predicting the relapse rates of patients with testicular germ cell tumors.

Int Immunopharmacol 2020 Jul 8;86:106710. Epub 2020 Jul 8.

Department of Urology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China. Electronic address:

Objective: The activities of tumor-infiltrating immune cells (TIICs) play an important role in the outcomes of many types of cancers. Here, we sought to describe the landscape of TIICs in testicular germ cell tumors (TGCT) and to develop a prognostic model based on this information.

Methods: The Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) algorithm was used to determine the proportions of 22 types of TIICs in a TGCT dataset (n = 74). Read More

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http://dx.doi.org/10.1016/j.intimp.2020.106710DOI Listing

Combined blockade of EGFR and glutamine metabolism in preclinical models of colorectal cancer.

Transl Oncol 2020 Jul 8;13(10):100828. Epub 2020 Jul 8.

Vanderbilt Center for Molecular Probes, Vanderbilt University Medical Center, 1161 21(st) Avenue South, Medical Center North, R0102, Nashville, TN 37232, United States; Vanderbilt University Institute of Imaging Science, Vanderbilt University Medical Center, 1161 21st Avenue South, Medical Center North, R0102, Nashville, TN 37232, United States; Department of Radiology and Radiological Sciences, Vanderbilt University Medical Center, 1161 21st Avenue South, Medical Center North, Nashville, TN 37232, United States; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, 2220 Pierce Avenue, Nashville, TN 37232, United States. Electronic address:

Improving response to epidermal growth factor receptor (EGFR)-targeted therapies in patients with advanced wild-type (WT) RAS colorectal cancer (CRC) remains an unmet need. In this preclinical work, we evaluated a new therapeutic combination aimed at enhancing efficacy by targeting cancer cell metabolism in concert with EGFR. We hypothesized that combined blockade of glutamine metabolism and EGFR represents a promising treatment approach by targeting both the "fuel" and "signaling" components that these tumors need to survive. Read More

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http://dx.doi.org/10.1016/j.tranon.2020.100828DOI Listing

CpG-coated Prussian blue nanoparticles-based photothermal therapy combined with anti-CTLA-4 immune checkpoint blockade triggers a robust abscopal effect against neuroblastoma.

Transl Oncol 2020 Jul 8;13(10):100823. Epub 2020 Jul 8.

The George Washington Cancer Center, The George Washington University, Washington, DC 20052, USA; The Institute for Biomedical Sciences, The George Washington University, Washington, DC 20037, USA; Department of Medicine, The George Washington University, Washington, DC 20037, USA. Electronic address:

High-risk neuroblastoma, which is associated with regional and systemic metastasis, is a leading cause of cancer-related mortality in children. Responding to this need for novel therapies for high-risk patients, we have developed a "nanoimmunotherapy," which combines photothermal therapy (PTT) using CpG oligodeoxynucleotide-coated Prussian blue nanoparticles (CpG-PBNPs) combined with anti-CTLA-4 (aCTLA-4) immunotherapy. Our in vitro studies demonstrate that in addition to causing ablative tumor cell death, our nanoimmunotherapy alters the surface levels of co-stimulatory, antigen-presenting, and co-inhibitory molecules on neuroblastoma tumor cells. Read More

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http://dx.doi.org/10.1016/j.tranon.2020.100823DOI Listing

Quantifying DNA damage induced by ionizing radiation and hyperthermia using single DNA molecule imaging.

Transl Oncol 2020 Jul 8;13(10):100822. Epub 2020 Jul 8.

Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden. Electronic address:

Ionizing radiation (IR) is a common mode of cancer therapy, where DNA damage is the major reason of cell death. Here, we use an assay based on fluorescence imaging of single damaged DNA molecules isolated from radiated lymphocytes, to quantify IR induced DNA damage. The assay uses a cocktail of DNA-repair enzymes that recognizes and excises DNA lesions and then a polymerase and a ligase incorporate fluorescent nucleotides at the damage sites, resulting in a fluorescent "spot" at each site. Read More

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http://dx.doi.org/10.1016/j.tranon.2020.100822DOI Listing

Candidate biomarker assessment for pharmacological response.

Transl Oncol 2020 Jul 8;13(10):100830. Epub 2020 Jul 8.

Developmental Therapeutic Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.

Using the information from our CellMiner (https://discover.nci.nih. Read More

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http://dx.doi.org/10.1016/j.tranon.2020.100830DOI Listing

Pamiparib is a potent and selective PARP inhibitor with unique potential for the treatment of brain tumor.

Neoplasia 2020 Jul 8;22(9):431-440. Epub 2020 Jul 8.

Department of In Vivo Pharmacology, BeiGene (Beijing) Co., Ltd., Beijing, PR China. Electronic address:

Pamiparib, an investigational Poly (ADP-ribose) polymerase (PARP) inhibitor in clinical development, demonstrates excellent selectivity for both PARP1 and PARP2, and superb anti-proliferation activities in tumor cell lines with BRCA1/2 mutations or HR pathway deficiency (HRD). Pamiparib has good bioavailability and is 16-fold more potent than olaparib in an efficacy study using BRCA1 mutated MDA-MB-436 breast cancer xenograft model. Pamiparib also shows strong anti-tumor synergy with temozolomide (TMZ), a DNA alkylating agent used to treat brain tumors. Read More

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http://dx.doi.org/10.1016/j.neo.2020.06.009DOI Listing

A case of one lung adenocarcinoma patient harboring a novel FAM179A-ALK (F1, A19) rearrangement responding to lorlatinib treatment.

Lung Cancer 2020 Jun 24;147:26-29. Epub 2020 Jun 24.

Department of Oncology, The 904 Hospital of the Joint Logistic Support Force of the Chinese People's Liberation Army, Wuxi, Jiangsu, 214044, PR China. Electronic address:

The FAM179A gene has recently been screened as a new fusion partner fusing to the anaplastic lymphoma kinase gene (ALK) in plasma cell-free DNA (cfDNA) of patients with non-small-cell lung cancer (NSCLC). However, the response of patients with NSCLC harboring the FAM179A-ALK fusion to ALK inhibitors remains unknown. In this study we report a novel FAM179A-ALK rearrangement variant (F1, A19) identified by next-generation sequencing in an NSCLC patient with multiple brain metastases (M1c). Read More

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http://dx.doi.org/10.1016/j.lungcan.2020.06.026DOI Listing

Pembrolizumab-induced severe oral mucositis in a patient with squamous cell carcinoma of the lung: A case study.

Lung Cancer 2020 Jun 30;147:21-25. Epub 2020 Jun 30.

Division of Medical Oncology-Hematology, Department of Internal Medicine, Kyung Hee University Hospital, College of Medicine, Kyung Hee University, Seoul, Republic of Korea. Electronic address:

Background: Immune checkpoint inhibitors, such as pembrolizumab, a humanized monoclonal antibody against programmed death-1, elicit antitumor activity in various types of cancers, including lung cancer. However, pembrolizumab has been reported to cause diverse immune-related adverse events associated with T-cell activation.

Case Presentation: We present the case of a 61-year-old man with advanced non-small cell lung cancer who was administered pembrolizumab as first-line treatment. Read More

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http://dx.doi.org/10.1016/j.lungcan.2020.06.033DOI Listing

IL-33/IL1RL1 axis regulates cell survival through the p38 MAPK pathway in acute myeloid leukemia.

Leuk Res 2020 Jul 2;96:106409. Epub 2020 Jul 2.

Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, PR China. Electronic address:

Acute myeloid leukemia (AML) is often characterized by the presence of specific and recurrent chromosomal abnormalities. Current treatments have greatly increased remission rate, but relapse still occurs. Therefore, novel therapeutic approaches are required. Read More

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http://dx.doi.org/10.1016/j.leukres.2020.106409DOI Listing

Design and evaluation a kind of functional biomaterial for bone tissue engineering: Selenium/mesoporous bioactive glass nanospheres.

J Colloid Interface Sci 2020 Jul 1;579:654-666. Epub 2020 Jul 1.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, People's Republic of China. Electronic address:

Conventional treatments of bone tumor involve removal followed by radiation and chemotherapeutic drugs that may have limitations and cause secondary damage. The development of functional filling biomaterial has led to a new strategy for tumor therapy. In this study, a novel therapeutic ion selenium doped mesoporous bioactive glasses (Se/MBG) nanospheres were successfully synthesized by a facile sol-gel technique using cetyl trimethyl ammonium bromide (CTAB) as the template, which had uniform spherical morphology (≈ 400 nm), high surface area (>400 m/g) and mesopore volume (≈0. Read More

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http://dx.doi.org/10.1016/j.jcis.2020.06.122DOI Listing

Tumour budding, poorly differentiated clusters, and T-cell response in colorectal cancer.

EBioMedicine 2020 Jul 8;57:102860. Epub 2020 Jul 8.

Program in MPE Molecular Pathological Epidemiology, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; Cancer Immunology and Cancer Epidemiology Programs, Dana-Farber Harvard Cancer Center, Boston, Massachusetts, USA. Electronic address:

Background: Tumour budding and poorly differentiated clusters (PDC) represent forms of tumour invasion. We hypothesised that T-cell densities (reflecting adaptive anti-tumour immunity) might be inversely associated with tumour budding and PDC in colorectal carcinoma.

Methods: Utilising 915 colon and rectal carcinomas in two U. Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102860DOI Listing

In vitro biological evaluation and consideration about structure-activity relationship of silver(I) aminoacidate complexes.

J Inorg Biochem 2020 Jul 3;210:111170. Epub 2020 Jul 3.

Department of Inorganic Chemistry, Faculty of Science, P. J. Šafárik University, Moyzesova 11, SK-041 54 Košice, Slovak Republic. Electronic address:

Two silver(I) aminoacidate complexes {[Ag(L-HAla)(NO)]NO} (AgAla, complex 1, Ala = alanine) and {[Ag(L-Phe)]} (AgPhe, complex 2, Phe = phenylalanine) were prepared and characterized by elemental, spectral analysis (FT-IR, NMR techniques) and single crystal X-ray analysis in solid state and their solution stability was measured in biological testing time-scale by H NMR. The bridging coordination modes of the zwitterionic Ala and deprotonated Phe ligands led to the formation of 1D polymeric chains of the complexes. The significant argentophilic interactions are presented in the structure of AgAla. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2020.111170DOI Listing

Development, evaluation and effect of anionic co-ligand on the biological activity of benzothiazole derived copper(II) complexes.

J Inorg Biochem 2020 Jul 3;210:111174. Epub 2020 Jul 3.

Department of Chemistry, School of Chemical Sciences and Pharmacy, Central University of Rajasthan, Bandarsindri, District Ajmer, Rajasthan 305817, India. Electronic address:

Research on development of novel metal based anti-cancer agents continues with its popularity among bioinorganic community. Benzothiazole, an important heterocyclic pharmacophore, was chosen as a valuable and useful scaffold for the synthesis of novel copper(II) complexes. Three new copper(II) complexes obtained from the synthesis of newly synthesized benzothiazole based N-(benzo[d]thiazol-2-ylmethyl)-N-methyl-2-(pyridin-2-yl)ethan-1-amine (btzpy) ligand with CuCl [Cu(btzpy)Cl] (1), Cu(NCS) [Cu(btzpy)(NCS)] (2), and Cu(NO) [Cu(btzpy)(NO)(HO)]NO (3) were isolated and characterized by physical and spectroscopic measurements, including single-crystal X-ray structures. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2020.111174DOI Listing

Iron promotes breast cancer cell migration via IL-6/JAK2/STAT3 signaling pathways in a paracrine or autocrine IL-6-rich inflammatory environment.

J Inorg Biochem 2020 Jun 24;210:111159. Epub 2020 Jun 24.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China; Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Iron overload can act as catalyst for the formation of free radicals, which may promote oxidant-mediated breast carcinogenesis. However, the association between iron and breast cancer has not been comprehensively elucidated. In this study, we found that iron overload upregulated the inflammatory cytokine interleukin-6 (IL-6) expression to activate Janus Kinases 2/Signal Transducer and Activator of Transcription 3 (JAK2/STAT3) signaling in triple negative breast cancer (TNBC) MDA-MB-231 cell lines, resulting in epithelial-mesenchymal transition (EMT) and cancer cell migration, but it had no effects on the estrogen receptor (ER)-positive breast cancer MCF-7 cells. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2020.111159DOI Listing

Controlled Release of Soy Isoflavones from 3D Printed Bone Scaffolds for Bone Tissue Engineering.

Acta Biomater 2020 Jul 8. Epub 2020 Jul 8.

W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States. Electronic address:

Recent challenges in post-surgical bone tumor management have elucidated the need for a multifunctional scaffold, which can be used for residual tumor-cell suppression, defect repair, and simultaneous bone regeneration. In this perspective, 3D printing allows to create a wide variety of patient-specific implant with complex porous architecture and compatible mechanical strength to that of cancellous bone. Here, a multifunctional bone graft substitute is designed by incorporating the three primary soy isoflavones: genistein, daidzein, and glycitein onto a 3D printed (3DP) tricalcium phosphate (TCP) scaffolds with designed pores, endowing them with in vitro chemopreventive, bone-cell proliferating and immune-modulatory potential. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.07.006DOI Listing

Osimertinib improves overall survival in EGFR-mutated non-small cell lung cancer patients with leptomeningeal metastases regardless of T790M mutational status.

J Thorac Oncol 2020 Jul 8. Epub 2020 Jul 8.

Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. Electronic address:

Introduction: Osimertinib, a third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), efficiently penetrates the blood-brain barrier. Current study explored whether treatment with osimertinib leads to improved overall survival (OS) for EGFR-mutated NSCLC patients with leptomeningeal metastases (LM) compared with those not treated with osimertinib.

Methods: From October 2008 to October 2019, patients with EGFR-mutated NSCLC and cytologically confirmed LM were retrospectively analyzed for OS according to osimertinib treatment and T790M mutational status. Read More

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http://dx.doi.org/10.1016/j.jtho.2020.06.018DOI Listing

Overexpression of the Tuberous sclerosis complex 2 (TSC2) gene inhibits goat myoblasts proliferation and differentiation in understanding the underlying mechanism of muscle development.

Gene 2020 Jul 8:144943. Epub 2020 Jul 8.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

The growth of animal skeletal muscle is mainly determined by the synthesis processes of total proteins in skeletal muscle cells, which has a significant impact on the postnatal growth of young animals. An increasing number of studies are focusing on the functions of Tuberous sclerosis complex 2 (TSC2) during the process of cell protein synthesis and growth. However, it is still unclear the effect of whether and how TSC2 on goat myoblasts proliferation and differentiation. Read More

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http://dx.doi.org/10.1016/j.gene.2020.144943DOI Listing

The Effect of Tumor Size and Histology on Outcomes Following Segmentectomy vs. Lobectomy for Clinically Node-negative Non-small Cell Lung Cancer.

Chest 2020 Jul 8. Epub 2020 Jul 8.

Division of Cardiovascular and Thoracic Surgery, Department of Surgery, Duke University Medical Center, Durham, NC.

Background: The interaction between tumor size and the comparative prognosis of lobar and sublobar resection has been poorly defined.

Research Question: The purpose of this study was to characterize the relationship between tumor size and the receipt of segmentectomy or lobectomy in association with overall survival in patients with clinically node-negative non-small cell lung cancer (NSCLC).

Study Design: and Methods: The 2004-2015 National Cancer Database (NCDB) was queried for patients with cT1-3N0M0 NSCLC who underwent segmentectomy or lobectomy without neoadjuvant therapy or missing survival data. Read More

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http://dx.doi.org/10.1016/j.chest.2020.06.066DOI Listing

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

Dev Cell 2020 Jul 8. Epub 2020 Jul 8.

MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Crewe Road South, Edinburgh EH4 2XU, UK; Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK. Electronic address:

Melanocytes, replenished throughout life by melanocyte stem cells (MSCs), play a critical role in pigmentation and melanoma. Here, we reveal a function for the metastasis-associated phosphatase of regenerating liver 3 (PRL3) in MSC regeneration. We show that PRL3 binds to the RNA helicase DDX21, thereby restricting productive transcription by RNAPII at master transcription factor (MITF)-regulated endolysosomal vesicle genes. Read More

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http://dx.doi.org/10.1016/j.devcel.2020.06.013DOI Listing

Dietary Lipids Induce Ferroptosis in Caenorhabditiselegans and Human Cancer Cells.

Dev Cell 2020 Jun 30. Epub 2020 Jun 30.

School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USA. Electronic address:

Dietary lipids impact development, homeostasis, and disease, but links between specific dietary fats and cell fates are poorly understood. Ferroptosis is an iron-dependent form of nonapoptotic cell death associated with oxidized polyunsaturated phospholipids. Here, we show that dietary ingestion of the polyunsaturated fatty acid (PUFA) dihomogamma-linolenic acid (DGLA; 20:3n-6) can trigger germ-cell ferroptosis and sterility in the nematode Caenorhabditis elegans. Read More

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http://dx.doi.org/10.1016/j.devcel.2020.06.019DOI Listing

A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation.

Nucleic Acids Res 2020 Jul 11. Epub 2020 Jul 11.

The Medical Research Council Cancer Unit, University of Cambridge, Hills Road, Cambridge CB2 0XZ, UK.

The catalytic activity of human AURORA-A kinase (AURKA) regulates mitotic progression, and its frequent overexpression in major forms of epithelial cancer is associated with aneuploidy and carcinogenesis. Here, we report an unexpected, kinase-independent function for AURKA in DNA replication initiation whose inhibition through a class of allosteric inhibitors opens avenues for cancer therapy. We show that genetic depletion of AURKA, or its inhibition by allosteric but not catalytic inhibitors, blocks the G1-S cell cycle transition. Read More

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http://dx.doi.org/10.1093/nar/gkaa570DOI Listing

Tumor Organoids to Study Gastroesophageal Cancer: A Primer.

J Mol Cell Biol 2020 Jul 11. Epub 2020 Jul 11.

Division of Gastroenterology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, USA.

Gastroesophageal cancers are leading causes of cancer death. Our attempts at adopting molecularly based treatment approaches have been slow and ineffective even though we begin to identify specific targetable gene mutations and pathways. It is clear that we should no longer treat all gastroesophageal cancers as a homogeneous disease, which is what we do when we use non-specific chemotherapy. Read More

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http://dx.doi.org/10.1093/jmcb/mjaa035DOI Listing

Melatonin protects against apoptosis of megakaryocytic cells via its receptors and the AKT/mitochondrial/caspase pathway.

Aging (Albany NY) 2020 Jul 10;12. Epub 2020 Jul 10.

Nanfang Hospital, Southern Medical University, Guangzhou, China.

Clinical studies have shown that melatonin lowers the frequency of thrombocytopenia in patients with cancer undergoing radiotherapy or chemotherapy. Here, we investigated the mechanisms by which melatonin promotes platelet formation and survival. Our results show that melatonin exerted protective effects on serum-free induced apoptosis of CHRF megakaryocytes (MKs). Read More

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http://dx.doi.org/10.18632/aging.103483DOI Listing

ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression.

Bosn J Basic Med Sci 2020 Jun 22. Epub 2020 Jun 22.

Department of Pathology, Pingshan District People's Hospital of Shenzhen, Shenzhen, China.

Breast cancer is one of the most prevalent malignancies in women worldwide. Although great progress has been achieved in the diagnosis and treatment of breast cancer, the prognosis of patients with breast cancer is still poor due to distal recurrence and metastasis after surgery. This study demonstrated that the expression level of ankyrin repeat domain 22 (ANKRD22) in human breast cancer tissues was significantly higher than that in normal breast tissues. Read More

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http://dx.doi.org/10.17305/bjbms.2020.4701DOI Listing

LAPTM4B promotes the progression of nasopharyngeal cancer.

Bosn J Basic Med Sci 2020 Jul 2. Epub 2020 Jul 2.

Department of Radiotherapy, Gansu Provincial Cancer Hospital, Lanzhou, China.

Lysosomal protein transmembrane 4 beta (LAPTM4B) is a protein that contains four transmembrane domains. The impact of LAPTM4B on the malignancy of nasopharyngeal carcinoma (NPC) remains unclear. In the present study, we aimed to investigate the role of LAPTM4B in NPC. Read More

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http://dx.doi.org/10.17305/bjbms.2020.4738DOI Listing

Solid Tumor Metastases to the Pancreas Diagnosed Using Fine-Needle Aspiration.

Am J Clin Pathol 2020 Jul 11. Epub 2020 Jul 11.

Department of Anatomical Pathology, Hepatology and Nutrition, University of Texas MD Anderson Cancer Center, Houston.

Objectives: Solid tumor metastases to the pancreas are rare, and only limited literature on the topic is available. In this retrospective study, we reviewed 107 cases of solid tumor metastases to the pancreas diagnosed by fine-needle aspiration (FNA) from 2005 to 2019.

Methods: Information including the patients' clinical histories, radiologic and pathologic findings, treatments, and follow-up were collected. Read More

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http://dx.doi.org/10.1093/ajcp/aqaa090DOI Listing

Bevacizumab in recurrent ovarian cancer: could it be particularly effective in patients with clear cell carcinoma?

Clin Transl Oncol 2020 Jul 10. Epub 2020 Jul 10.

Department of Medical Oncology, Hospital Universitario La Paz, Paseo de la Castellana 261, 28046, Madrid, Spain.

Purpose: Treatment of recurrent ovarian carcinoma is a challenge, particularly for the clear cell (CCC) subtype. However, there is a preclinical rationale that these patients could achieve a benefit from antiangiogenic therapy. To assess this hypothesis, we used the growth modulation index (GMI), which represents an intrapatient comparison of two successive progression-free survival (PFS). Read More

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http://dx.doi.org/10.1007/s12094-020-02446-zDOI Listing

Hyperprogressive disease in patients with advanced renal cell carcinoma: a new pattern of post-treatment cancer behavior.

Immunol Res 2020 Jul 10. Epub 2020 Jul 10.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, 200080, People's Republic of China.

Renal cell carcinoma (RCC) is among the most common cancers of the genitourinary system. Once RCC has progressed to a high tumor stage, surgery is no longer the optimal option, and treatment with drugs is more suitable. However, a proportion of patients with advanced RCC (aRCC) experience accelerated progression following targeted therapy or immunotherapy, a condition known as hyperprogressive disease (HPD). Read More

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http://dx.doi.org/10.1007/s12026-020-09138-4DOI Listing

Immunotherapy in Anal Cancer.

Curr Oncol Rep 2020 Jul 11;22(9):94. Epub 2020 Jul 11.

Montefiore Medical Center, Bronx, NY, USA.

Purpose Of Review: Standard treatment for early-stage squamous cell cancer of the anal canal (SCCA) includes concurrent chemotherapy and radiation to achieve curative intent. Treatment options are limited, however, especially with locoregional disease relapse occurring in 20-30% of patients and about 10-30% of patients presenting with metastatic disease. With more than 90% of SCCAs occurring in the setting of HPV, immune-based therapies are now the target of possible new treatments for this rare disease. Read More

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http://dx.doi.org/10.1007/s11912-020-00946-3DOI Listing

Anti-transcriptional intermediary factor 1-γ antibody-positive dermatomyositis induced by nivolumab for lung adenocarcinoma: A case report.

Invest New Drugs 2020 Jul 10. Epub 2020 Jul 10.

Department of Respiratory Medicine, Kobe City Medical Center General Hospital, 2-1-1, Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.

Immune checkpoint inhibitors can induce immune-related adverse events (irAEs) in different organs. Dermatomyositis is a rare form of systemic irAE. Although dermatomyositis-specific antibodies, especially anti-transcriptional intermediary factor 1-γ (anti-TIF1-γ) antibodies, have been detected in a few cases of immune checkpoint inhibitor-associated dermatomyositis, their titers before immunotherapy have not been examined. Read More

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http://dx.doi.org/10.1007/s10637-020-00974-7DOI Listing

Cancer immunosensor based on apo and holo transferrin binding.

Mikrochim Acta 2020 Jul 10;187(8):438. Epub 2020 Jul 10.

Chemistry Department, Campus CEDETEG, State University of Midwest Paraná, Guarapuava, Brazil.

An electrochemical immunosensor was developed for the determination of apo-Tf (non-iron-bound) and holo-Tf (iron-bound) using polyclonal antibody transferrin (anti-Tf) immobilized at an electrode surface as a biorecognition platform. The monitoring was based on the anti-Tf binding with both Tf forms which allows the detection of cancer cells due to the constant iron cycle and the overexpression of anti-Tf on the cancer cell surface. The immunosensor characterization was performed using electrochemical impedance spectroscopy (EIS), which evaluated the impedimetric biorecognition of the antigens-antibody by the use of KFe(CN) redox group. Read More

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http://dx.doi.org/10.1007/s00604-020-04420-6DOI Listing

Effect of Ag-1031 on apoptosis in gastric cancer AGS cells and its effects on the PI3K/AKT/mTOR signaling pathway.

Biotechnol Lett 2020 Jul 10. Epub 2020 Jul 10.

Department of Hepatobiliary Surgery, The Second People's Hospital of Wuhu, No. 259, Jiuhua Middle Road, Wuhu, 241001, Anhui, China.

Purpose: To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway.

Methods: Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Read More

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http://dx.doi.org/10.1007/s10529-020-02954-6DOI Listing

Ga-FAPi-46 diffuse bilateral breast uptake in a patient with cervical cancer after hormonal stimulation.

Eur J Nucl Med Mol Imaging 2020 Jul 10. Epub 2020 Jul 10.

Ahmanson Translational Theranostics Division, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095-7370, USA.

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http://dx.doi.org/10.1007/s00259-020-04947-zDOI Listing

Selective BET-bromodomain inhibition by JQ1 suppresses dendritic cell maturation and antigen-specific T-cell responses.

Cancer Immunol Immunother 2020 Jul 10. Epub 2020 Jul 10.

Department of Oncology, Hematology, Immuno-Oncology and Rheumatology, University Hospital of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.

Bromo- and extra-terminal domain (BET) inhibitors represent potential therapeutic approaches in solid and hematological malignancies that are currently analyzed in several clinical trials. Additionally, BET are involved in the epigenetic regulation of immune responses by macrophages and dendritic cells (DCs), that play a central role in the regulation of immune responses, indicating that cancer treatment with BET inhibitors can promote immunosuppressive effects. The aim of this study was to further characterize the effects of selective BET inhibition by JQ1 on DC maturation and DC-mediated antigen-specific T-cell responses. Read More

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http://dx.doi.org/10.1007/s00262-020-02665-xDOI Listing

Do Not Minimize the Impact of Cellular Immunity in the Development of Vaccines and Therapeutics for COVID-19.

J Infect Dis 2020 Jul 11. Epub 2020 Jul 11.

Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

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http://dx.doi.org/10.1093/infdis/jiaa415DOI Listing

Cell death in the gut epithelium and implications for chronic inflammation.

Nat Rev Gastroenterol Hepatol 2020 Jul 10. Epub 2020 Jul 10.

Department of Medicine 1, Friedrich-Alexander University, Erlangen, Germany.

The intestinal epithelium has one of the highest rates of cellular turnover in a process that is tightly regulated. As the transit-amplifying progenitors of the intestinal epithelium generate ~300 cells per crypt every day, regulated cell death and sloughing at the apical surface keeps the overall cell number in check. An aberrant increase in the rate of intestinal epithelial cell (IEC) death underlies instances of extensive epithelial erosion, which is characteristic of several intestinal diseases such as inflammatory bowel disease and infectious colitis. Read More

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http://dx.doi.org/10.1038/s41575-020-0326-4DOI Listing

Pan-RAF inhibition induces apoptosis in acute myeloid leukemia cells and synergizes with BCL2 inhibition.

Leukemia 2020 Jul 10. Epub 2020 Jul 10.

Institute for Molecular Medicine Finland, Helsinki Institute of Life Science, University of Helsinki, Helsinki, Finland.

Pan-RAF inhibitors have shown promise as antitumor agents in RAS and RAF mutated solid cancers. However, the efficacy of pan-RAF inhibitors in acute myeloid leukemia (AML) has not previously been explored. In AML, the RAS-RAF-MEK-ERK (MAPK) pathway is one of the most aberrantly activated oncogenic pathways, but previous targeting of this pathway by MEK inhibitors has not proven effective in clinical trials. Read More

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http://dx.doi.org/10.1038/s41375-020-0972-0DOI Listing

Association of progression-free or event-free survival with overall survival in diffuse large B-cell lymphoma after immunochemotherapy: a systematic review.

Leukemia 2020 Jul 10. Epub 2020 Jul 10.

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Collaborative Innovation Center for Cancer Medicine, Beijing, P.R. China.

To investigate progression-free survival (PFS) and event-free survival (EFS) as early efficacy endpoints in diffuse large B-cell lymphoma (DLBCL), this systematic review included phase III randomized controlled trials (RCTs), phase II trials, and retrospective studies in newly diagnosed DLBCL receiving rituximab-containing chemotherapy through databases search up to 2019. Quality control was performed, where studies with high risk of bias were excluded. Prediction models were first established using the RCTs, and then externally validated in the phase II and retrospective populations. Read More

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http://dx.doi.org/10.1038/s41375-020-0963-1DOI Listing
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