300,298 results match your criteria Cancer biology & medicine[Journal]


Systematic Analysis of the Aberrances and Functional Implications of Ferroptosis in Cancer.

iScience 2020 Jun 20;23(7):101302. Epub 2020 Jun 20.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China; Precision Diagnosis and Treatment for Gastrointestinal Cancer, Chinese Academy of Medical Sciences, Guangzhou 510060, China. Electronic address:

Ferroptosis is a type of cell death related to cancer; however, the characteristics of ferroptosis in cancers are still uncertain. Based on the data in The Cancer Genome Atlas, we found that most ferroptosis regulator genes (FRGs) were differentially expressed in tumors, somatic copy number alterations (SCNA) and DNA methylation contributed to their aberrant expression. We established the ferroptosis potential index (FPI) to reveal the functional roles of ferroptosis and noticed that the FPI was higher in tumors than in normal tissues in most cancers and was associated with subtypes and clinical features. Read More

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http://dx.doi.org/10.1016/j.isci.2020.101302DOI Listing

Cu-selective NIR fluorescence sensor based on heptamethine cyanine in aqueous media and its application.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Jun 19;240:118606. Epub 2020 Jun 19.

Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand. Electronic address:

A near-infrared (NIR) colorimetric fluorescence sensor, Cy7C3, based on heptamethine cyanine dye was synthesized for determining the presence of Cu ions. The sensor showed highly sensitive fluorescence quenching toward Cu ions in acetonitrile/buffer solution at physiological pH with long emission wavelength of 718 nm. Cy7C3 also provided an excellent selectivity to Cu ions over other competing metal ions, with a low detection limit of 9 ppb, which was lower than the maximum concentration of Cu ions in drinking water of U. Read More

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http://dx.doi.org/10.1016/j.saa.2020.118606DOI Listing

Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma.

EBioMedicine 2020 Jul 3;57:102846. Epub 2020 Jul 3.

Department of Internal Medicine, National Taiwan University Hospital, No. 7, Zhongshan S Rd, Zhongzheng District, Taipei 10002, Taiwan; Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No. 1, Jen Ai Rd, Section 1, Zhongzheng District, Taipei 10051, Taiwan. Electronic address:

Background: Aberrant fucosylation plays a critical role in lung cancer progression. Nevertheless, the key fucosyltransferase with prognostic significance in lung cancer patients, the enzyme's intracellular targets, and complex molecular mechanisms underlying lung cancer metastasis remain incompletely understood.

Methods: We performed a large-scale transcriptome-clinical correlation to identify major fucosyltransferases with significant prognostic values. Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102846DOI Listing

Single cell genomics and developmental biology: moving beyond the generation of cell type catalogues.

Curr Opin Genet Dev 2020 Jul 3;64:66-71. Epub 2020 Jul 3.

Department of Haematology, University of Cambridge, Cambridge, UK; Wellcome-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. Electronic address:

Major developmental processes such as gastrulation and early embryogenesis rely on a complex network of cell-cell interactions, chromatin remodeling, and transcriptional regulators. This makes it challenging to study early development when using bulk populations of cells. Recent advances in single-cell technologies have allowed researchers to better understand the interactions between different molecular modalities and the heterogeneities within classically defined cell types. Read More

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http://dx.doi.org/10.1016/j.gde.2020.05.033DOI Listing

TROY signals through JAK1-STAT3 to promote glioblastoma cell migration and resistance.

Neoplasia 2020 Jul 3;22(9):352-364. Epub 2020 Jul 3.

Department of Cancer Biology, Mayo Clinic Arizona, Scottsdale, AZ 85259, United States. Electronic address:

Glioblastoma (GBM) is the most common primary malignant brain tumor in adults and carries a discouraging prognosis. Its aggressive and highly infiltrative nature renders the current standard treatment of maximal surgical resection, radiation, and chemotherapy relatively ineffective. Identifying the signaling pathways that regulate GBM migration/invasion and resistance is required to develop more effective therapeutic regimens to treat GBM. Read More

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http://dx.doi.org/10.1016/j.neo.2020.06.005DOI Listing

Modulating the modulators: regulation of protein arginine methyltransferases by post-translational modifications.

Drug Discov Today 2020 Jul 3. Epub 2020 Jul 3.

Department of Pharmacology and Toxicology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA; Department of Medical and Molecular Genetics, 975 W. Walnut Street, Indianapolis, IN 46202, USA. Electronic address:

The therapeutic potential of targeting protein arginine methyltransferases (PRMTs) is inextricably linked to their key roles in various cellular functions, including splicing, proliferation, cell cycle regulation, differentiation, and DNA damage signaling. Unsurprisingly, the development of inhibitors against these enzymes has become a rapidly expanding research area. However, effective targeting of PRMTs requires a deeper understanding of the mechanistic details behind their regulation at multiple levels, involving those mechanisms that alter their activity, interactions, and localization. Read More

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http://dx.doi.org/10.1016/j.drudis.2020.06.031DOI Listing

Characterization of a polysaccharide from Sanghuangporus vaninii and its antitumor regulation via activation of the p53 signaling pathway in breast cancer MCF-7 cells.

Int J Biol Macromol 2020 Jul 3. Epub 2020 Jul 3.

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address:

Sanghuangporus is a traditional medicine that has been used for nearly 2000 years in Asia. It has been found to enhance immunity and have anticancer properties. Some studies determined that Sanghuangporus polysaccharide can inhibit tumors in vitro, although the underlying mechanisms remain unknown. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.279DOI Listing

Impact of IL1R1 polymorphisms on the risk of head and neck cancer in Chinese Han population.

Gene 2020 Jul 3:144927. Epub 2020 Jul 3.

Central Laboratory, Hainan General Hospital, No.19, Xiuhua Road, Xiuying District, Haikou City, 570311; Key Laboratory of Cell and Molecular Genetic Translational Medicine in Hainan Province, Hainan General Hospital, No.19, Xiuhua Road, Xiuying District, Haikou City, 570311. Electronic address:

Aim: The incidence of head and neck cancer (HNC) is increasing but its pathogenic factors are complex. Changes in both internal (genetic) and external (environmental) causes HNC to some extent. The purpose of our study was to investigate the influence of IL1R1 polymorphisms on HNC risk in Chinese Han population. Read More

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http://dx.doi.org/10.1016/j.gene.2020.144927DOI Listing

Coronaviruses and Integrin αvβ3: Does Thyroid Hormone Modify the Relationship?

Endocr Res 2020 Aug;45(3):210-215

Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences , Rensselaer, NY, USA.

Background: Uptake of coronaviruses by target cells involves binding of the virus by cell ectoenzymes. For the etiologic agent of COVID-19 (SARS-CoV-2), a receptor has been identified as angiotensin-converting enzyme-2 (ACE2). Recently it has been suggested that plasma membrane integrins may be involved in the internalization and replication of clinically important coronaviruses. Read More

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http://dx.doi.org/10.1080/07435800.2020.1767127DOI Listing

Preclinical insights into the gut-skeletal muscle axis in chronic gastrointestinal diseases.

J Cell Mol Med 2020 Jul 6. Epub 2020 Jul 6.

Department of Medicine II, Division of Gastroenterology, Rostock University Medical Center, Rostock, Germany.

Muscle wasting represents a constant pathological feature of common chronic gastrointestinal diseases, including liver cirrhosis (LC), inflammatory bowel diseases (IBD), chronic pancreatitis (CP) and pancreatic cancer (PC), and is associated with increased morbidity and mortality. Recent clinical and experimental studies point to the existence of a gut-skeletal muscle axis that is constituted by specific gut-derived mediators which activate pro- and anti-sarcopenic signalling pathways in skeletal muscle cells. A pathophysiological link between both organs is also provided by low-grade systemic inflammation. Read More

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http://dx.doi.org/10.1111/jcmm.15554DOI Listing

Evaluation of an aldo-keto reductase gene signature with prognostic significance in colon cancer via activation of epithelial to mesenchymal transition and the p70S6K pathway.

Carcinogenesis 2020 Jul 6. Epub 2020 Jul 6.

Department of Biological Sciences, Orta Dogu Teknik Universitesi, Ankara, Turkey.

AKR1B1 and AKR1B10, members of the aldo-keto reductase family of enzymes that participate in the polyol pathway of aldehyde metabolism, are aberrantly expressed in colon cancer. We previously showed that high expression of AKR1B1 (AKR1B1HIGH) was associated with enhanced motility, inflammation and poor clinical outcome in colon cancer patients. Using publicly available datasets and ex vivo gene expression analysis (n=51, Ankara cohort), we have validated our previous in silico finding that AKR1B1HIGH was associated with worse overall survival (OS) compared to patients with low expression of AKR1B1 (AKR1B1LOW) samples. Read More

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http://dx.doi.org/10.1093/carcin/bgaa072DOI Listing

Astrocytes influence medulloblastoma phenotypes and CD133 surface expression.

PLoS One 2020 6;15(7):e0235852. Epub 2020 Jul 6.

Department of Pediatrics, Division of Neonatology, Developmental Vascular Biology Program, Children's Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.

The medulloblastoma (MB) microenvironment is diverse, and cell-cell interactions within this milieu is of prime importance. Astrocytes, a major component of the microenvironment, have been shown to impact primary tumor cell phenotypes and metastasis. Based on proximity of MB cells and astrocytes in the brain microenvironment, we investigated whether astrocytes may influence MB cell phenotypes directly. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235852PLOS

Anticancer Activity Assessment and DNA Binding Properties of Two Binuclear Platinum (II) Complexes using Spectroscopic and Molecular Simulation Approaches.

Anticancer Agents Med Chem 2020 Jul 5. Epub 2020 Jul 5.

Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran. Iran.

Background: Nowadays, the biological properties and anticancer activities of platinum-based drugs and metal coordination complexes have received particular attention. These compounds have revealed the clinical potential in cancer chemotherapy.

Objective: In this research, the anticancer activity and molecular simulation of two newly synthesized binuclear platinum complexes involving [Pt2Cl2(bhq)2(µ-dppm)] (1) and [(p-MeC6H4)(bhq) Pt(µ-dppm)Pt(bhq)(CF3CO2)] (2) in which bhq: benzo[h] quinolone and dppm: bis(diphenylphosphino) methane have been described. Read More

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http://dx.doi.org/10.2174/1871520620666200705221325DOI Listing

Intracellular signaling modules linking DNA damage to secretome changes in senescent melanoma cells.

Melanoma Res 2020 Aug;30(4):336-347

aCutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA bÉcole Polytechnique Universitaire Polytech'Nice-Sophia, Sophia Antipolis, France.

Cellular senescence is a major barricade on the path of cancer development, yet proteins secreted from senescent cells exert complex and often discordant effects on subsequent cancer evolution. Somatic genome alternations driving the formation of nevi and melanoma are efficient inducers of cellular senescence. Melanocyte and melanoma cell senescence is likely to come into play as a key factor affecting the course of tumorigenesis and responsiveness to therapy; little mechanistic information has been generated, however, that substantiates this idea and facilitates its clinical translation. Read More

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http://dx.doi.org/10.1097/CMR.0000000000000671DOI Listing

Loss of Metabolic Flexibility due to Overexpression of PDKs in Muscle, Liver, and the Immune System: Therapeutic Targets in Metabolic Diseases.

J Diabetes Investig 2020 Jul 6. Epub 2020 Jul 6.

Leading-Edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu, Republic of Korea.

Good health depends upon maintenance of metabolic flexibility which in turn is dependent upon maintenance of regulatory flexibility of a large number of regulatory enzymes but especially the pyruvate dehydrogenase complex (PDC) due to its central role in carbohydrate metabolism. Flexibility in regulation of PDC is dependent upon rapid changes in the phosphorylation state of PDC determined by the relative activities of the pyruvate dehydrogenase kinases (PDKs) and the pyruvate dehydrogenase phosphatases (PDPs). Inactivation of the PDC by overexpression of PDK4 contributes to the hyperglycemia and therefore the serious health problems associated with diabetes. Read More

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http://dx.doi.org/10.1111/jdi.13345DOI Listing

Curcumin downregulates the PI3K-AKT-mTOR pathway and inhibits growth and progression in head and neck cancer cells.

Phytother Res 2020 Jul 6. Epub 2020 Jul 6.

Epithelial Biology Laboratory, Department of Periodontics and Oral Medicine, Division of Oral Pathology Oral Radiology and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.

Curcumin, a polyphenol isolated from the rhizome of Curcuma longa, has been studied because of its antioxidant, antimicrobial, and antiinflammatory properties. This study aimed to evaluate the effects of curcumin on head and neck cancer (HNC) cell lines and how it modulates the PI3K-AKT-mTOR signaling pathway. Dose-response curves for curcumin were established for hypopharynx carcinoma (FaDu), tongue carcinoma (SCC-9), and keratinocytes (HaCaT) cell lines and IC values were calculated. Read More

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http://dx.doi.org/10.1002/ptr.6780DOI Listing

ERBB2b mRNA isoform encodes a nuclear variant of the ERBB2 oncogene in breast cancer.

J Cell Biochem 2020 Jul 6. Epub 2020 Jul 6.

INSERM UMR1090 TAGC, Aix-Marseille University, Marseille, France.

The presence of nuclear ERBB2 receptor-type tyrosine kinase is one of the causes of the resistance to membrane ERBB2-targeted therapy in breast cancers. It has been previously reported that this nuclear location arises through at least two different mechanisms: proteolytic shedding of the extracellular domain of the full-length receptor and translation of the messenger RNA (mRNA)-encoding ERBB2 from internal initiation codons. Here, we report a new mechanism and function where a significant portion of nuclear ERBB2 results from the translation of the variant ERBB2 mRNA under the transcriptional control of a distal promoter that is actively used in breast cancer cells. Read More

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http://dx.doi.org/10.1002/jcb.29762DOI Listing

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury.

J Vis Exp 2020 Jun 17(160). Epub 2020 Jun 17.

Department of Urology, Boston Children's Hospital; Department of Surgery, Harvard Medical School;

We describe the implementation of spinal cord injury in mice to elicit detrusor-sphincter dyssynergia, a functional bladder outlet obstruction, and subsequent bladder wall remodeling. To facilitate assessment of the cellular composition of the bladder wall in non-injured control and spinal cord injured mice, we developed an optimized dissociation protocol that supports high cell viability and enables the detection of discrete subpopulations by flow cytometry. Spinal cord injury is created by complete transection of the thoracic spinal cord. Read More

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http://dx.doi.org/10.3791/61455DOI Listing

PTEN and DNA-PK determine sensitivity and recovery in response to WEE1 inhibition in human breast cancer.

Elife 2020 Jul 6;9. Epub 2020 Jul 6.

Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

Inhibition of WEE1 kinase by AZD1775 has shown promising results in clinical cancer trials, but markers predicting AZD1775 response are lacking. Here we analysed AZD1775 response in a panel of human breast cancer (BC) cell lines by global proteome/transcriptome profiling and identified two groups of basal-like BC (BLBCs): 'PTEN low' BLBCs were highly sensitive to AZD1775 and failed to recover following removal of AZD1775, while 'PTEN high' BLBCs recovered. AZD1775 induced phosphorylation of DNA-PK, protecting cells from replication-associated DNA damage and promoting cellular recovery. Read More

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http://dx.doi.org/10.7554/eLife.57894DOI Listing

Drug mechanism-of-action discovery through the integration of pharmacological and CRISPR screens.

Mol Syst Biol 2020 Jul;16(7):e9405

Wellcome Sanger Institute, Hinxton, UK.

Low success rates during drug development are due, in part, to the difficulty of defining drug mechanism-of-action and molecular markers of therapeutic activity. Here, we integrated 199,219 drug sensitivity measurements for 397 unique anti-cancer drugs with genome-wide CRISPR loss-of-function screens in 484 cell lines to systematically investigate cellular drug mechanism-of-action. We observed an enrichment for positive associations between the profile of drug sensitivity and knockout of a drug's nominal target, and by leveraging protein-protein networks, we identified pathways underpinning drug sensitivity. Read More

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http://dx.doi.org/10.15252/msb.20199405DOI Listing

The Sca-1 and Sca-1 mouse prostatic luminal cell lineages are independently sustained.

Stem Cells 2020 Jul 6. Epub 2020 Jul 6.

Department of Urology, University of Washington, Seattle, WA.

The phenotypic and functional heterogeneity of the mouse prostate epithelial cell lineages remains incompletely characterized. We show that the Sca-1 luminal cells at the mouse proximal prostate express Sox2. These cells are replicative quiescent, castration resistant, and do not possess secretory function. Read More

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http://dx.doi.org/10.1002/stem.3253DOI Listing

Nanoparticle-based biosensors for detection of extracellular vesicles in liquid biopsies.

J Mater Chem B 2020 Jul 6. Epub 2020 Jul 6.

Aragón Materials Science Institute (ICMA), CSIC/University of Zaragoza, Zaragoza, Spain. and Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.

Tumor-derived extracellular vesicles have emerged as an alternative source of cancer biomarkers in liquid biopsies. Despite their clinical potential, traditional methods for isolation and analysis have hampered their translation into the clinic. The use of nanomaterial-based biosensors can speed up the development of analytical methods for quantifying extracellular vesicles in a specific, highly reproducible, robust, fast and inexpensive way. Read More

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http://dx.doi.org/10.1039/d0tb00861cDOI Listing

Association of IGF-1 gene rs2195239 polymorphism with the risk and clinical features of gastric cancer in a Chinese Han population.

J Clin Lab Anal 2020 Jul 5:e23436. Epub 2020 Jul 5.

Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.

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http://dx.doi.org/10.1002/jcla.23436DOI Listing

Fasten the seat belt: Increasing safety of CAR T-cell therapy.

Exp Dermatol 2020 Jul 5. Epub 2020 Jul 5.

Department of Dermatology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Universitätsklinikum Erlangen, Erlangen, Germany.

After the recent success and approvals of chimeric antigen receptor (CAR) T cells in haematological malignancies, its efficacy is currently evaluated in a broad spectrum of tumor entities including melanoma. However, severe and potentially life-threatening side effects like cytokine release syndrome, neurologic toxicities, and the competing risk of morbidity and mortality from the treatment itself are still a major limiting factor in the current CAR T-cell landscape. In addition, especially in solid tumors, the lack of ideal target antigens to avoid on-target/off-tumor toxicities also restricts its use. Read More

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http://dx.doi.org/10.1111/exd.14131DOI Listing

The usefulness of periostin determination in gynecology and obstetrics.

Ginekol Pol 2020 ;91(6):364-351

Department of Physiotherapy, Higher School of Physical Education, Wroclaw, Poland.

Periostin (POSTN) is a multifunctional glycoprotein that belongs to the group of extracellular matrix (ECM) proteins. Due to the molecular structure, cellular interactions, tissue locations as well functions of POSTN, we realize that its pivotal role is organization and regulation of ECM microenvironment. In available databases there is a lack of data summarizing current knowledge about POSTN expression in the field of gynecology and obstetrics. Read More

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http://dx.doi.org/10.5603/GP.2020.0064DOI Listing
January 2020

Expression profile of stem cell markers and ABC transporters in 5-fluorouracil resistant Hep-2 cells.

Mol Biol Rep 2020 Jul 5. Epub 2020 Jul 5.

Department of Molecular Biology and Genetics, Erzurum Technical University, Omer Nasuhi Bilmen Mah. Havaalani Yolu Cad. No: 53 Yakutiye, Erzurum, Turkey.

Resistance of laryngeal squamous cell carcinoma cells to traditional therapeutic regimens still remains to be a major reason for therapeutic failure in patients. In this study, we aimed at investigating the expression profiles of ATP-binding cassette (ABC) transporters and stem cell markers in 5-fluorouracil (5-FU) resistant laryngeal Hep-2 cells. We treated parental Hep-2 cells, with stepwise increased doses of 5-FU for almost 1 year to develop 5-FU resistant sub-lines with resistance against varying levels of 5-FU concentrations (4 sub-lines resistant to 1, 2, 4, and eightfold of 5-FU). Read More

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http://dx.doi.org/10.1007/s11033-020-05633-xDOI Listing

Possible involvement of TGF‑β‑SMAD‑mediated epithelial‑mesenchymal transition in pro‑metastatic property of PAX6.

Oncol Rep 2020 Aug 11;44(2):555-564. Epub 2020 Jun 11.

Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250103, P.R. China.

Paired box 6 (PAX6) is a transcription factor that has oncogenic features. In breast cancer, PAX6 facilitates tumor progression; however, the underlying mechanism is largely unknown. The majority of breast cancer‑related mortalities are associated with metastasis of cancer cells. Read More

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http://dx.doi.org/10.3892/or.2020.7644DOI Listing

Identification of TYMS as a promoting factor of retroperitoneal liposarcoma progression: Bioinformatics analysis and biological evidence.

Oncol Rep 2020 Aug 5;44(2):565-576. Epub 2020 Jun 5.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Hepato‑Pancreato‑Biliary Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China.

Retroperitoneal liposarcoma (RLPS) is one of the most common types of retroperitoneal sarcomas, and has a high recurrence rate. There is an urgent need to further explore its pathogenesis and develop more effective treatment strategies. The aim of the present study was to identify potential driver genes of RLPS through bioinformatics analysis and molecular biology to elucidate potential targets that are suitable for further analysis for the treatment of RLPS. Read More

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http://dx.doi.org/10.3892/or.2020.7635DOI Listing

Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation.

Int J Mol Med 2020 Aug 3;46(2):546-560. Epub 2020 Jun 3.

CONACyT, Metabolomics and Proteomics Laboratory, Academic Unit of Biological Sciences, Universidad Autónoma de Zacatecas, Zacatecas 98068, Mexico.

Placenta‑derived exosomes play an important role in cellular communication both in the mother and the fetus. Their concentration and composition are altered in several pregnancy disorders, such as gestational diabetes mellitus (GDM). The isolation and characterization of placental exosomes from serum, plasma and tissues from patients with GDM have been previously described; however, to the best of our knowledge, to date, there is no study available on placental exosomes isolated from urine of patients with GDM. Read More

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http://dx.doi.org/10.3892/ijmm.2020.4626DOI Listing

Isoorientin induces the apoptosis and cell cycle arrest of A549 human lung cancer cells via the ROS‑regulated MAPK, STAT3 and NF‑κB signaling pathways.

Int J Oncol 2020 Aug 9;57(2):550-561. Epub 2020 Jun 9.

Department of Biochemistry and Molecular Biology, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P.R. China.

Isoorientin (ISO) is a naturally occurring C‑glycosyl flavone that has various pharmacological properties, such as anti‑bacterial and anti‑inflammatory effects. However, its underlying molecular mechanisms in human lung cancer cells remain unknown. In the present study, the effects of ISO on the induction of apoptosis and relative molecular mechanisms in A549 human lung cancer cells were investigated. Read More

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http://dx.doi.org/10.3892/ijo.2020.5079DOI Listing

Alternative Polyadenylation Modification Patterns Reveal Essential Posttranscription Regulatory Mechanisms of Tumorigenesis in Multiple Tumor Types.

Biomed Res Int 2020 15;2020:6384120. Epub 2020 Jun 15.

School of Life Sciences, Shanghai University, Shanghai 200444, China.

Among various risk factors for the initiation and progression of cancer, alternative polyadenylation (APA) is a remarkable endogenous contributor that directly triggers the malignant phenotype of cancer cells. APA affects biological processes at a transcriptional level in various ways. As such, APA can be involved in tumorigenesis through gene expression, protein subcellular localization, or transcription splicing pattern. Read More

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http://dx.doi.org/10.1155/2020/6384120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315320PMC

Structural and Functional Insight Into the Glycosylation Impact Upon the HGF/c-Met Signaling Pathway.

Front Cell Dev Biol 2020 18;8:490. Epub 2020 Jun 18.

College of Medicine, Hunan Normal University, Changsha, China.

Upon interactions with its specific ligand hepatocyte growth factor (HGF), the c-Met signal is relayed to series of downstream pathways, exerting essential biological roles. Dysregulation of the HGF-c-Met signaling pathway has been implicated in the onset, progression and metastasis of various cancers, making the HGF-c-Met axis a promising therapeutic target. Both c-Met and HGF undergo glycosylation, which appears to be biologically relevant to their function and structural integrity. Read More

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http://dx.doi.org/10.3389/fcell.2020.00490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314907PMC

The Relevance of Transcription Factors in Gastric and Colorectal Cancer Stem Cells Identification and Eradication.

Front Cell Dev Biol 2020 18;8:442. Epub 2020 Jun 18.

i3S - Institute for Research and Innovation in Health, University of Porto, Porto, Portugal.

Gastric and colorectal cancers have a high incidence and mortality worldwide. The presence of cancer stem cells (CSCs) within the tumor mass has been indicated as the main reason for tumor relapse, metastasis and therapy resistance, leading to poor overall survival. Thus, the elimination of CSCs became a crucial goal for cancer treatment. Read More

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http://dx.doi.org/10.3389/fcell.2020.00442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314965PMC

Early T Stage Is Associated With Poor Prognosis in Patients With Metastatic Liver Colorectal Cancer.

Front Oncol 2020 18;10:716. Epub 2020 Jun 18.

Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, Chinese National Ministry of Education and Key Laboratory of Molecular Biology in Medical Sciences), Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Clinically, a considerable portion of patients with early T stage who were supposed to have a low distant metastatic probability were diagnosed with metastatic liver colorectal cancer (CRLM). Our study aims to evaluate the prognostic value of the T stage for metastatic patients and establish a convenient individual assessment model for clinicians to explore preoperative predictors. The mRNA profiles of colorectal tumors ( = 19) were obtained by microarray at our clinical center. Read More

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http://dx.doi.org/10.3389/fonc.2020.00716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314979PMC

Correction: MiR-940 inhibits TGF-β-induced epithelial-mesenchymal transition and cell invasion by targeting Snail in non-small cell lung cancer.

J Cancer 2020 11;11(16):4897-4898. Epub 2020 Jun 11.

Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou 215004, China.

[This corrects the article DOI: 10.7150/jca.31800. Read More

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http://dx.doi.org/10.7150/jca.48686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330705PMC

Long noncoding RNA PANDAR inhibits the development of lung cancer by regulating autophagy and apoptosis pathways.

J Cancer 2020 6;11(16):4783-4790. Epub 2020 Jun 6.

The State Key Lab of Respiratory Disease, The First Affiliated Hospital, The institute for Chemical Carcinogenesis, School of Public health, Guangzhou Medical University, Guangzhou, China.

LncRNAs has been shown to play important roles in the progression of lung cancer, but it remains poorly understood whether lncRNAs affect the occurrence and development of lung cancer by regulating autophagy and apoptosis levels. Here, we investigated the roles of PANDAR in NSCLC. The expression profile and clinical application of PANDAR and its possible target gene BECN1 were tested in 276 cases of lung cancer tissues. Read More

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http://dx.doi.org/10.7150/jca.45291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330687PMC

Time-resolved multi-omics analysis reveals the role of nutrient stress-induced resource reallocation for TAG accumulation in oleaginous fungus .

Biotechnol Biofuels 2020 1;13:116. Epub 2020 Jul 1.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave, Wuxi, 214122 Jiangsu People's Republic of China.

Background: Global resource reallocation is an established critical strategy through which organisms deal with environmental stress. The regulation of intracellular lipid storage or utilization is one of the most important strategies for maintaining energy homeostasis and optimizing growth. Oleaginous microorganisms respond to nitrogen deprivation by inducing lipid hyper accumulation; however, the associations between resource allocation and lipid accumulation are poorly understood. Read More

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http://dx.doi.org/10.1186/s13068-020-01757-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328260PMC

Emerging Roles of Mast Cells in the Regulation of Lymphatic Immuno-Physiology.

Front Immunol 2020 17;11:1234. Epub 2020 Jun 17.

Department of Medical Physiology, Texas A&M University Health Science Center College of Medicine, Bryan, TX, United States.

Mast cells (MCs) are abundant in almost all vascularized tissues. Furthermore, their anatomical proximity to lymphatic vessels and their ability to synthesize, store and release a large array of inflammatory and vasoactive mediators emphasize their significance in the regulation of the lymphatic vascular functions. As a major secretory cell of the innate immune system, MCs maintain their steady-state granule release under normal physiological conditions; however, the inflammatory response potentiates their ability to synthesize and secrete these mediators. Read More

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http://dx.doi.org/10.3389/fimmu.2020.01234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311670PMC

CAR-T Cells Hit the Tumor Microenvironment: Strategies to Overcome Tumor Escape.

Front Immunol 2020 17;11:1109. Epub 2020 Jun 17.

Department of Hematology and Oncology, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clinic, Barcelona, Spain.

Chimeric antigen receptor (CAR) T cell therapies have demonstrated remarkable efficacy for the treatment of hematological malignancies. However, in patients with solid tumors, objective responses to CAR-T cell therapy remain sporadic and transient. A major obstacle for CAR-T cells is the intrinsic ability of tumors to evade immune responses. Read More

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http://dx.doi.org/10.3389/fimmu.2020.01109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311654PMC

Polarization of Immune Cells in the Pathologic Response to Inhaled Particulates.

Authors:
Qiang Ma

Front Immunol 2020 17;11:1060. Epub 2020 Jun 17.

Receptor Biology Laboratory, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, United States.

Polarization of immune cells is commonly observed in host responses associated with microbial immunity, inflammation, tumorigenesis, and tissue repair and fibrosis. In this process, immune cells adopt distinct programs and perform specialized functions in response to specific signals. Accumulating evidence indicates that inhalation of micro- and nano-sized particulates activates barrier immune programs in the lung in a time- and context-dependent manner, including type 1 and type 2 inflammation, and T helper (Th) 17 cell, regulatory T cell (Treg), innate lymphoid cell (ILC), and myeloid-derived suppressor cell (MDSC) responses, which highlight the polarization of several major immune cell types. Read More

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http://dx.doi.org/10.3389/fimmu.2020.01060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311785PMC

-Methyl-D-Aspartate Receptors in Hematopoietic Cells: What Have We Learned?

Front Physiol 2020 17;11:577. Epub 2020 Jun 17.

Red Blood Cell Research Group, Institute of Veterinary Physiology, Vetsuisse Faculty, University of Zurich, Zürich, Switzerland.

The -methyl-D-aspartate receptor (NMDAR) provides a pathway for glutamate-mediated inter-cellular communication, best known for its role in the brain but with multiple examples of functionality in non-neuronal cells. Data previously published by others and us provided evidence that NMDARs regulate platelet and red blood cell (RBC) production. Here, we summarize what is known about these hematopoietic roles of the NMDAR. Read More

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http://dx.doi.org/10.3389/fphys.2020.00577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311790PMC

Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway.

Front Pharmacol 2020 18;11:864. Epub 2020 Jun 18.

Cardiovascular Department, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: M1 macrophage plays an important role in inflammatory reaction. In this study, potential anti-inflammatory effect of Fisch flavonoids (PCFF) was assessed Zebrafish acute inflammation model and LPS-induced pro-inflammatory M1 macrophage model .

Methods: The quality control of Fisch flavonoids (PCFF) was analyzed by HPLC. Read More

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http://dx.doi.org/10.3389/fphar.2020.00864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314944PMC

Evaluation of p16 expression as a single marker to select patients with HPV-driven oropharyngeal cancers for treatment de-escalation.

Br J Cancer 2020 Jul 6. Epub 2020 Jul 6.

Department of Otorhinolaryngology, Head and Neck Surgery, University of Giessen, Giessen, Germany.

Background: A remarkably better prognosis is associated with oropharyngeal squamous cell carcinomas (OPSCC) driven by human papillomaviruses (HPV) compared with HPV-negative OPSCC. Consequently, de-escalation of standard treatment has been suggested. Due to modest specificity rates, debates are ongoing, whether p16, a surrogate marker for HPV-driven OPSCC, is sufficient to correctly identify those tumours and avoid substantial HPV misattribution and thus undertreatment of patients by de-escalation. Read More

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http://dx.doi.org/10.1038/s41416-020-0964-xDOI Listing

Polygenic risk score opportunities for early detection and prevention strategies in endometrial cancer.

Br J Cancer 2020 Jul 6. Epub 2020 Jul 6.

Division of Cancer Sciences, Faculty of Biology, Medicine and Health, University of Manchester, St Mary's Hospital, Manchester, UK.

Recent large-scale genetic studies, particularly genome-wide association studies (GWAS), have emphasised the importance of common genetic variation in endometrial cancer susceptibility. Although each of these variants only confer modest effects on endometrial cancer risk, together they are likely to explain a substantial amount of the familial relative risk of the disease. Therefore, methods to combine genetic risk variants, such as polygenic risk scores (PRS) have gained traction as an attractive method for individualised risk prediction and management. Read More

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http://dx.doi.org/10.1038/s41416-020-0959-7DOI Listing

[Familial leukemia due to germline RUNX1 mutations: lessons learned from two decades of research and unsolved problems].

Rinsho Ketsueki 2020 ;61(6):687-696

Cancer Science Institute of Singapore, National University of Singapore.

The RUNX1 gene is a critical transcription factor for the generation and maintenance of hematopoietic stem cells. RUNX1 is also one of the most frequently mutated gene in sporadic leukemias. Heterozygous loss-of-function mutations of the RUNX1 gene in the germline cause a rare autosomal dominant disorder called familial platelet disorder with propensity to acute myelogenous leukemia (FPD/AML). Read More

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http://dx.doi.org/10.11406/rinketsu.61.687DOI Listing
January 2020

Reproductive outcomes after in vitro fertilization treatment in a cohort of Danish women transplanted with cryopreserved ovarian tissue.

Fertil Steril 2020 Jul 2. Epub 2020 Jul 2.

The Fertility Clinic, Skive Regional Hospital, Skive, Denmark; Faculty of Health, Aarhus University, Aarhus, Denmark.

Objective: To evaluate ovarian stimulation regimens and reproductive outcomes in a cohort of women undergoing ovarian tissue cryopreservation (OTC) and ovarian tissue transplantation (OTT).

Design: Retrospective cohort study.

Setting: University hospital and fertility clinics. Read More

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http://dx.doi.org/10.1016/j.fertnstert.2020.03.035DOI Listing

Survival of the cheapest: how proteome cost minimization drives evolution.

Authors:
Kasper P Kepp

Q Rev Biophys 2020 Jun 23;53:e7. Epub 2020 Jun 23.

Technical University of Denmark, DTU Chemistry, Kemitorvet 206, DK-2800 Kongens Lyngby, Denmark.

Darwin's theory of evolution emphasized that positive selection of functional proficiency provides the fitness that ultimately determines the structure of life, a view that has dominated biochemical thinking of enzymes as perfectly optimized for their specific functions. The 20th-century modern synthesis, structural biology, and the central dogma explained the machinery of evolution, and nearly neutral theory explained how selection competes with random fixation dynamics that produce molecular clocks essential e.g. Read More

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http://dx.doi.org/10.1017/S0033583520000037DOI Listing

The Role of Glypicans in Cancer Progression and Therapy.

J Histochem Cytochem 2020 Jul 6:22155420933709. Epub 2020 Jul 6.

Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

Glypicans are a family of heparan sulfate proteoglycans that are attached to the cell membrane via a glycosylphosphatidylinositol anchor. Glypicans interact with multiple ligands, including morphogens, growth factors, chemokines, ligands, receptors, and components of the extracellular matrix through their heparan sulfate chains and core protein. Therefore, glypicans can function as coreceptors to regulate cell proliferation, cell motility, and morphogenesis. Read More

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http://dx.doi.org/10.1369/0022155420933709DOI Listing

EpigenCentral: Portal for DNA methylation data analysis and classification in rare diseases.

Hum Mutat 2020 Jul 5. Epub 2020 Jul 5.

Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, Canada.

Epigenetic processes play a key role in regulating gene expression. Genetic variants that disrupt chromatin-modifying proteins are associated with a broad range of diseases, some of which have specific epigenetic patterns, such as aberrant DNA methylation, that may be used as disease biomarkers. While much of the epigenetic research has focused on cancer, there is a paucity of resources devoted to neurodevelopmental disorders, which include autism spectrum disorder and many rare, clinically overlapping syndromes. Read More

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http://dx.doi.org/10.1002/humu.24076DOI Listing

Variant effect on splicing regulatory elements, branchpoint usage, and pseudoexonization: strategies to enhance bioinformatic prediction using hereditary cancer genes as exemplars.

Hum Mutat 2020 Jul 5. Epub 2020 Jul 5.

Genetics and Computational Biology Department, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia.

It is possible to estimate the prior probability of pathogenicity for germline disease gene variants based on bioinformatic prediction of variant effect/s. However, routinely used approaches have likely led to the underestimation and underreporting of variants located outside donor and acceptor splice site motifs that affect mRNA processing. This review presents information about hereditary cancer gene germline variants, outside native splice sites, with experimentally validated splicing effects. Read More

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http://dx.doi.org/10.1002/humu.24074DOI Listing