9,884 results match your criteria Canadian journal of physiology and pharmacology[Journal]


Diabetes-related sex differences in the brain endothelin system following ischemia in vivo and in human brain endothelial cells in vitro.

Can J Physiol Pharmacol 2020 Jun 4. Epub 2020 Jun 4.

Medical University of South Carolina, 2345 , 171 Ashley AvenMSC 908 , Charleston, South Carolina, United States , 29425-2503.

The endothelin (ET) system has been implicated to contribute to the pathophysiology of cognitive impairment and stroke in experimental diabetes. Our goals were to test the hypotheses that 1) circulating and/or peri-infarct ET-1 levels are elevated after stroke in both sexes and this increase is greater in diabetes, 2) ET receptors are differentially regulated in the diabetic brain, 3) brain microvascular endothelial cells (BMVEC) of female and male origin express the ETA receptor subtype, and 4) diabetes and stroke-mimicking conditions increase ET-1 levels in BMVECs of both sexes. Control and diabetic rats were randomized to sham or stroke surgery. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0630DOI Listing

Gestational postprandial insulin sensitivity in the Sprague Dawley rat: the putative role of Hepatic Insulin Sensitizing Substance (HISS) in glucose partitioning in pregnancy.

Can J Physiol Pharmacol 2020 May 26. Epub 2020 May 26.

SciMar Ltd, Dauphin, Canada ;

Pregnancy requires adaptation of maternal insulin sensitivity. In the fed state, a pulse of insulin stimulates glucose uptake and nutrient energy storage via insulin-dependent as well as Hepatic Insulin Sensitizing Substance (HISS)-dependent action. HISS is released by the liver in the fed state in the presence of signals integrated through the liver and a pulse of insulin. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0575DOI Listing

History of the Pharmacological Society of Canada 1956-2008.

Can J Physiol Pharmacol 2020 May 25:343-350. Epub 2020 May 25.

Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, Ontario, Canada.

The Pharmacological Society of Canada (PSC) formed in 1956 and became a constituent society of the Canadian Federation of Biological Sciences (CFBS) in 1958. Over subsequent decades, it met annually with CFBS, matured as a society, and established an identity as the voice of pharmacology in Canada. During the 1980s, it sought a larger stage and bid for, and then hosted, the XIIth International Congress of Pharmacology in Montreal in 1994. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0380DOI Listing

Knockout of the Circadian Clock Protein PER1 Results in Sex-Dependent Alterations of ET-1 Production in Mice in Response to a High Salt Diet plus Mineralocorticoid Treatment.

Can J Physiol Pharmacol 2020 May 21. Epub 2020 May 21.

University of Florida, 3463, Medicine, Gainesville, Florida, United States ;

Previously, we showed that global knockout (KO) of the circadian clock transcription factor PER1 in male, but not female, mice fed a high salt diet plus mineralocorticoid treatment (HS/DOCP) resulted in non-dipping hypertension and decreased night/day ratio of sodium (Na) excretion. Additionally, we have shown that the endothelin-1 (ET-1) gene is targeted by both PER1 and aldosterone. We hypothesized that ET-1 would exhibit a sex-specific response to HS/DOCP treatment in PER1 KO. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0688DOI Listing

Predictive model of bosentan-induced liver toxicity in Japanese patients with pulmonary arterial hypertension.

Can J Physiol Pharmacol 2020 May 20. Epub 2020 May 20.

Kobe Pharmaceutical University, 12883, Clinical Pharmaceutical Science , 4-19-1, Motoyama-kitamachi, Higashinada , Kobe, Japan , 658-8588.

Bosentan, an endothelin receptor antagonist, has been widely used as a first-line medication for the treatment of pulmonary arterial hypertension (PAH). However, liver dysfunction is a major side effect of bosentan treatment that could hamper the optimal management of patients with PAH. Previously, we demonstrated, using drug metabolism enzymes and transporters (DMET) analysis, that the carbohydrate sulfotransferase 3 (CHST3) and CHST13 alleles are significantly more frequent in patients with elevated amino-transferases during therapy with bosentan than they are in patients without liver toxicity. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0656DOI Listing

Correction: Intracrine signaling through lipid mediators and their cognate nuclear G-protein-coupled receptors: a paradigm based on PGE, PAF, and LPA receptors.

Can J Physiol Pharmacol 2020 May 7. Epub 2020 May 7.

Departments of Pediatrics and Pharmacology, Research Center of Hôpital Sainte-Justine, 3175 Côte Sainte-Catherine, Université de Montréal, Montréal, QC H3T 1C5, Canada.

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http://dx.doi.org/10.1139/cjpp-2020-0206DOI Listing

Endokinin A/B stimulates rat gastric motility through myogenic NK1 receptors located in fundus.

Can J Physiol Pharmacol 2020 May 4. Epub 2020 May 4.

Lanzhou University, School of Life Sciences , 222 Tianshui South Road , Lanzhou, Gansu, China , 730000 ;

Endokinin A/B (EKA/B), the common C-terminal decapeptide in endokinins A and B, is a preferred ligand of NK1 receptor and regulates pain and itch. The study focused on the effects of EKA/B on rat gastric motility in vivo and in vitro. Gastric emptying was measured to evaluate gastric motility in vivo. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0522DOI Listing

Folic acid affects cardiometabolic, oxidative stress and immunohistochemical parameters in monocrotaline-induced rat heart failure.

Can J Physiol Pharmacol 2020 Apr 30. Epub 2020 Apr 30.

Institute of Medical Physiology "Richard Burian", Faculty of Medicine, University of Belgrade , str. Visegradska 26/2 , Belgrade, Serbia , 11000 ;

Heart failure (HF) is one of the major cardiovascular causes of death worldwide. In this study, we explored the effects of folic acid (FA) on cardiometabolic, oxidative stress-biomarker changes and the activity of proliferation marker Ki67 in monocrotaline-induced HF. The research was conducted during a 4-week period, using 5 experimental groups (8 animals/group): blank solution-exposed controls (C1 physiological saline 1ml/kg one day; C2 physiological saline 1ml/kg 28 days), MCT-induced HF (MCT 50mg/kg), FA (FA 5mg•kg-1•day-1) and MCT+FA (MCT 50mg/kg, FA 5mg•kg-1•day-1). Read More

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http://dx.doi.org/10.1139/cjpp-2020-0030DOI Listing

Endothelin-1, endothelin receptor antagonists and vein graft occlusion in coronary artery bypass surgery: twenty years on and still no journey from bench to bedside.

Can J Physiol Pharmacol 2020 Apr 28. Epub 2020 Apr 28.

University College London, 4919, Centre for Rheumatology , London, London, United Kingdom of Great Britain and Northern Ireland ;

The saphenous vein is the most commonly used bypass graft in patients with coronary artery disease. During routine coronary artery bypass grafting the vascular damage inflicted on the vein is likely to stimulate the release of endothelin-1, a potent endothelium-derived vasoconstrictor that also possesses cell proliferation and inflammatory properties, conditions associated with vein graft failure. In both in vitro and in vivo studies, endothelin receptor antagonists reduce neointimal thickening. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0598DOI Listing
April 2020
1.546 Impact Factor

The ratio of circulating endothelin-1:endothelin-3 associated with TIMI risk and dynamic TIMI risk score in ST elevation acute myocardial infarction.

Can J Physiol Pharmacol 2020 Apr 21. Epub 2020 Apr 21.

Universitas Gadjah Mada, 59166, Cardiology and Vascular Medicine, Yogyakarta, Indonesia ;

In ST segment elevation acute myocardial infarction (STEMI), the endothelin (ET) system imbalance, reflected by circulating ET-1:ET-3 ratio has not been investigated. This study primary objective was to measure the circulating ET-1:ET-3 ratio and correlated it with the risk stratification for 1-year mortality of STEMI, based on TIMI score. On admission TIMI risk score and at discharge dynamic TIMI risk score were calculated in 68 consecutive subjects with STEMI. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0654DOI Listing

Chondroitin sulfate mediates liver responses to injury induced by dual endothelin receptor inhibition.

Can J Physiol Pharmacol 2020 Apr 21. Epub 2020 Apr 21.

Kobe Pharmaceutical University, 12883, Clinical Pharmaceutical Science , 4-19-1, Motoyama-kitamachi, Higashinada , Kobe, Japan , 658-8588.

Although dual endothelin receptor antagonists (ERAs) show great promise for treating various conditions, their propensity to induce liver injury limits their clinical usage. Inflammation and fibrosis are important processes in liver responses to injury and it has been suggested that they and dual ERA-induced liver injury are mediated by the proteoglycan component chondroitin sulfate (CS), which is synthesized by CHST3 and CHST13. In this study we investigated whether dual ER inhibition in the liver could alter CHST3 and CHST13 expression and thus CS production, and whether liver CS content could prevent inflammatory and fibrosis responses after liver injury. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0649DOI Listing

Galectin-3 mediates high-glucose-induced cardiomyocyte injury by NADPH oxidase/reactive oxygen species pathway.

Can J Physiol Pharmacol 2020 Apr 20. Epub 2020 Apr 20.

Shandong Provincial Hospital, Jinan, China ;

Galectin-3 is a member of the β-galactoside-binding lectin family taken part in the regulation of inflammation, angiogenesis, and fibrosis. This study was designed to study the improved effect of galectin-3 inhibition on diabetic cardiomyopathy (DCM). Sprague-Dawley rats were randomized into the control, DCM, and DCM + modified citrus pectin (MCP, a galectin-3 pharmacological inhibitor) groups. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0708DOI Listing

Blocking properties of terguride at the 5-HT2 receptor subtypes mediating cardiovascular responses in the rat.

Can J Physiol Pharmacol 2020 Apr 8. Epub 2020 Apr 8.

Center for Research and Advanced Studies of the National Polytechnic Institute Department of Pharmacobiology South Campus, 505597, Pharmacobiology, Mexico city, Mexico ;

In vitro studies have suggested that terguride blocks the contractile and relaxant responses produced by 5-hydroxytryptamine (5-HT) via 5-HT2A/2B receptors. This study has now investigated terguride's blocking properties on central/peripheral 5-HT2 receptors in a cardiovascular preparation in anaesthetized or pithed rats. Male Wistar anaesthetized/pithed rats were cannulated for recording blood pressure and heart rate and for i. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0591DOI Listing

miR-142-5p and miR-212-5p cooperatively inhibit the proliferation and collagen formation of cardiac fibroblasts by regulating c-Myc/TP53INP1.

Can J Physiol Pharmacol 2020 May 8;98(5):314-323. Epub 2020 Apr 8.

Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang; 050000, Hebei Province, China.

The aim of this study was to investigate the effect and mechanism of miR-142-5p/212-5p on the proliferation and collagen formation of cardiac fibroblasts (CFs) after myocardial infarction (MI). The mouse MI model was established by ligation of the left anterior descending coronary artery. CFs were induced by transforming growth factor-beta 1 (TGF-β1) or angiotensin (Ang II). Read More

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http://dx.doi.org/10.1139/cjpp-2019-0495DOI Listing

CIRCULATING ENDOTHELIAL CELL-DERIVED MICROVESICLES ARE ELEVATED WITH HYPERTENSION AND ASSOCIATED WITH ENDOTHELIAL DYSFUNCTION.

Can J Physiol Pharmacol 2020 Apr 8. Epub 2020 Apr 8.

University of Colorado at Boulder , Integrative Vascular Biology Laboratory , Department of Integrative Physiology , Boulder, Colorado, United States , 80309-0354 ;

The purpose of this study was to determine: 1) if circulating endothelial microvesicles (EMVs) are elevated in hypertensive adults; and 2) whether circulating EMVs are associated with hypertension-related endothelial vasodilator dysfunction. Circulating EMVs (CD31+/42b-) was determined in 30 middle-aged adults (55+1 years): 15 normotensive (10M/5F; BP: 114/71+2/1 mmHg) and 15 hypertensive (10M/5F; 142/87+2/2 mmHg). Forearm blood flow (FBF: via plethysmography) was assessed by intra-arterial infusion of acetylcholine and sodium nitroprusside. Read More

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http://dx.doi.org/10.1139/cjpp-2020-0044DOI Listing

C-type natriuretic peptide (CNP)/Guanylate cyclase B (GC-B) system and endothelin-1(ET-1)/ET receptor A and B system in human vasculature.

Can J Physiol Pharmacol 2020 Apr 8. Epub 2020 Apr 8.

Kyoto University , 54 Sakyo-ku, Kawahara-cho , Kyoto, Japan ;

In order to assess the physiological and clinical implications of C-type natriuretic peptide (CNP)/guanylyl cyclase B (GC-B) system in the human vasculature, we have examined gene expressions of CNP and its receptor, GC-B, in human vascular endothelial cells (ECs) and smooth muscle cells (SMCs) and have also compared endothelin-1(ET-1)/endothelin receptor-A (ETR-A) and endothelin receptor-B (ETR-B) system in human aortic ECs (HAECs) and vascular SMCs (HSMCs) in vitro. We also examined these gene expressions in human embryonic stem (ES)/induced pluripotent stem cell (iPS)-derived ECs and mural cells (MCs). A little but significant amount of mRNA encoding CNP was detected in both human ES-derived ECs and HAECs. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0686DOI Listing

Dexamethasone causes defective glucose-6-phosphate dehydrogenase-dependent antioxidant barrier through endoglin in pregnant and non-pregnant rats.

Can J Physiol Pharmacol 2020 Apr 7. Epub 2020 Apr 7.

University of Ilorin, 108285, 1HOPE Cardiometabolic Research Team and Department of Physiology, College of Health Sciences, Ilorin, Kwara, Nigeria ;

Glucocorticoid (GC) therapy has been associated with adverse cardiometabolic effects during pregnancy. Inflammation-mediated cardiac dysfunction, an independent risk factor for morbidity and mortality, has been linked to defective glucose-6-phosphate dehydrogenase (G6PD)-dependent antioxidant defenses and increased endoglin expression. We therefore sought to investigate the effects of dexamethasone (DEX) on cardiac endoglin and G6PD-dependent antioxidant defense. Read More

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http://dx.doi.org/10.1139/cjpp-2018-0351DOI Listing

THIRTY-DAY EXPERIMENTAL DIABETES IMPAIRS CONTRACTILITY AND INCREASES FATIGUE RESISTANCE IN RAT DIAPHRAGM MUSCLE ASSOCIATED WITH INCREASED ANTI-OXIDATIVE ACTIVITY.

Can J Physiol Pharmacol 2020 Apr 3. Epub 2020 Apr 3.

Federal University of Parana, 28122, Physiology, Curitiba, PR, Brazil ;

Diabetes Mellitus (DM) is a metabolic disorder which can generate tissue damage through several pathways. Alteration and dysfunction of skeletal muscle are reported including respiratory muscles, which may compromise respiratory parameters in diabetic patients. We aimed to evaluate the diaphragm muscle contractility, tissue remodeling, oxidative stress and inflammatory parameters from 30 days streptozotocin-treated rats. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0609DOI Listing

A comparative, single-blind, randomized study on quetiapine and aripiperazole augmentation in treatment of selective serotonin reuptake inhibitor refractory obsessive-compulsive disorder.

Can J Physiol Pharmacol 2020 Apr 31;98(4):236-242. Epub 2020 Mar 31.

Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Obsessive-compulsive disorder (OCD) is a chronic psychiatric disorder, of unknown etiology, that affects 2.5% of the population. An appropriate therapeutic response to conventional treatment is seen. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0381DOI Listing

Temperature-dependent effects on CO water bath therapy induced changes in blood flow and vascularity in hind limb ischemia.

Can J Physiol Pharmacol 2020 Apr 24;98(4):228-235. Epub 2020 Mar 24.

Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Department of Physiology and Pathophysiology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.

To test if magnitudes of the beneficial actions of CO water bath therapy on blood flow and vascular density are dependent upon temperature, ischemia in the hind limb of rats was induced by occluding the left femoral artery for 2 weeks and the animals were exposed to water bath therapy with or without CO at 34 or 41 °C for 4 weeks (20 min treatment each day for 5 days/week). CO water bath therapy at 34 °C increased peak, minimal, and mean blood flow by 190%-600% in the ischemic limb. On the other hand, CO water bath treatment at 41 °C increased these parameters of blood flow by 37%, 55%, and 41%, respectively, in the ischemic limb. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0537DOI Listing

Selective perfusion of coronary vasculature in preterm sheep: a methodological innovation undermined by unfavourable operation of the foramen ovale.

Can J Physiol Pharmacol 2020 Apr 23;98(4):211-218. Epub 2020 Mar 23.

Fondazione Toscana Gabriele Monasterio, 54100 Massa and 56100 Pisa, Italy.

Antenatal cardiac intervention affords new prospects for hypoplastic left heart syndrome. Its success, however, may come not only from absence of impediments to blood flow but also from a sufficiently developed cardiac wall. Here, we examined the feasibility to perfuse selectively the fetal coronary circulation for treatment with growth promoting agents. Read More

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http://dx.doi.org/10.1139/cjpp-2018-0648DOI Listing

SET8 participates in lipopolysaccharide-mediated BV2 cells inflammation via modulation of TICAM-2 expression.

Can J Physiol Pharmacol 2020 Mar 16. Epub 2020 Mar 16.

1. Department of Anesthesiology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China., Shanghai, China ;

Microglia inflammation, involved in the occurrence and development of sepsis-associated encephalopathy, exhibits upregulation of pro-inflammatory cytokines and pro-inflammatory enzymes expression, leading to inflammation-induced neuronal cell apoptosis. TIR domain-containing adapter molecule-2 (TICAM-2), participates in lipopolysaccharide (LPS)-mediated BV2 cells inflammation. SET8 plays a crucial role in a variety of cellular signal pathways. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0699DOI Listing

Astragaloside IV relieves gestational diabetes mellitus in genetic mice through reducing hepatic gluconeogenesis.

Can J Physiol Pharmacol 2020 Mar 11. Epub 2020 Mar 11.

Cangzhou Central Hospital, Cangzhou , China ;

Background The glucose intolerance developed during pregnancy is called gestational diabetes mellitus (GDM). GDM has become a severe risk for the health of both mother and baby. Astragaloside IV (AS-IV) is the dominant active component in Astragalus membranaceus and has been reported to have anti-inflammation and immune-regulation function. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0548DOI Listing

Gut microbiota dysbiosis in Polycystic ovary syndrome (PCOS): association with obesity - A preliminary report.

Can J Physiol Pharmacol 2020 Mar 9. Epub 2020 Mar 9.

Southeast University Zhongda Hospital, 162752, Nanjing, Jiangsu, China ;

Objectives: To explore if and how the microbiota changed in PCOS women against healthy women.

Methods: Eight obese PCOS (PO group), ten non-obese PCOS (PN group) and nine healthy normal-weight women (control) (C group) were enrolled. Insulin (INS), testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2) and dehydroepiandrosterone (DHEA) were detected with radioimmunoassay. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0413DOI Listing

Why the endothelium? The endothelium as a target to reduce diabetes-associated vascular disease.'

Can J Physiol Pharmacol 2020 Mar 9. Epub 2020 Mar 9.

Dept of Pharmacology, Faculty of Medicine, University of Calgary, , 3300 Hospital Drive, NW , Calgary, Alberta, Canada , T2N4N1 ;

Over the past 66 years our knowledge of the role of endothelium in the regulation of cardiovascular function and dysfunction has advanced from the assumption that it is a single layer of cells that serves as a barrier between the blood stream and vascular smooth muscle, to an understanding of its role as an essential endocrine-like organ. In terms of historical contributions, we pay particular credit to: 1. The Canadian scientist, Dr. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0677DOI Listing

Retraction: Epigallocatechin gallate effectively ameliorates fluoride-induced oxidative stress and DNA damage in the liver of rats.

Can J Physiol Pharmacol 2020 03;98(3):182

Department of Zoology, Annamalai University, Annamalainagar 608002, Tamilnadu, India.

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http://dx.doi.org/10.1139/cjpp-2020-0072DOI Listing

Long-term survival outcome for pre-capillary pulmonary hypertension at a Japanese single center.

Can J Physiol Pharmacol 2020 Mar 3. Epub 2020 Mar 3.

Division of Cardiovascular Medicine, Department of Medicine, Kurume University School of Medicine, Kurume, Japan ;

In recent years, several treatment options for patients with pre-capillary pulmonary hypertension (PH) have improved the short-term prognosis. However, the long-term survival for pre-capillary PH has not been well investigated. This study sought to investigate the long-term survival for pre-capillary PH in Kurume University Hospital. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0663DOI Listing

The effect of Gender and ABCB1 Gene Polymorphism on the Pharmacokinetics of Azithromycin in Healthy Male and Female Pakistani Subjects.

Can J Physiol Pharmacol 2020 Mar 3. Epub 2020 Mar 3.

Kohat University of Science and Technology, 66860, Kohat, Pakistan ;

Background: In the current study, the possible outcome of sex difference and genotypic polymorphism of ABCB1 gene encoding p-glycoprotein (involved in biliary excretion of azithromycin) on the pharmacokinetics of azithromycin has been evaluated.

Methods: An open-label, the comparative pharmacokinetic study was arranged by including female and male healthy Pakistani volunteers. They were administered with a single oral dose of azithromycin 500 mg. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0569DOI Listing

Upregulation of PACE4 in Prostate Cancer is not dependent on E2F Transcription Factors.

Can J Physiol Pharmacol 2020 Mar 2. Epub 2020 Mar 2.

University of Sherbrooke Faculty of Medicine and Health Sciences, 12370, Department of Surgery , 3001, 12e Ave. Nord , Sherbrooke, Quebec, Canada , J1H5N4 ;

Recent studies in prostate cancer (PCa) have identified PACE4, a proprotein convertase enzyme as an emerging therapeutic target. Inhibition of PACE4-altCT, an oncogenic isoform of PACE4, using molecular or pharmacological approaches results in decreased cell proliferation and tumor progression in xenograft models. Although several validations have confirmed PACE4-altCT as a novel therapeutic target, the transcriptional regulation of PACE4 isoforms and mechanism of action remain a challenge. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0668DOI Listing

Endothelin-1 (ET-1) Promotes a Proinflammatory Microglia Phenotype in Diabetic Conditions.

Can J Physiol Pharmacol 2020 Mar 2. Epub 2020 Mar 2.

Medical University of South Carolina, 2345 , 171 Ashley AvenMSC 908 , Charleston, South Carolina, United States , 29425-2503.

Diabetes increases the risk and severity of cognitive impairment, especially after ischemic stroke. It is also known that the activation of ET system is associated with cognitive impairment and microglia around periinfarct area produce ET-1. However, little is known about the effect of ET-1 on microglial polarization, especially under diabetic conditions. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0679DOI Listing

Combined treatments with metformin and phosphodiesterase inhibitors alleviate non-alcoholic fatty liver disease in high-fat diet-fed rats: A comparative study.

Can J Physiol Pharmacol 2020 Feb 21. Epub 2020 Feb 21.

Faculty of Pharmacy, Minia University, El-Minia, Egypt ;

Nonalcoholic fatty liver disease (NAFLD) is an excessive accumulation of fats in the liver resulted in hepatic inflammation and fibrous tissue formation along with insulin resistance. This study was designed to investigate the possible protective effects of metformin alone and in combination with different phosphodiesterase inhibitors (PDEIs). Rats were fed a high-fat diet (HFD) for sixteen weeks to induce NAFLD. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0487DOI Listing
February 2020

Loss of endothelin type B receptor function improves insulin sensitivity in rats.

Can J Physiol Pharmacol 2020 Feb 21. Epub 2020 Feb 21.

University of Mississippi Medical Center, 21693 , 2500 North State St. , G558 , Jackson, United States , 39216-4505 ;

High salt intake (HS) is associated with obesity and insulin resistance. ET-1, a peptide released in response to HS, inhibits the actions of insulin on cultured adipocytes through ET-1 type B (ETB) receptors; however, the in vivo implications of ETB receptor activation on lipid metabolism and insulin resistance is unknown. We hypothesized that activation of ETB receptors in response to HS intake promotes dyslipidemia and insulin resistance. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0666DOI Listing
February 2020

Ketamine attenuates high-glucose-mediated endothelial inflammation in human umbilical vein endothelial cells.

Can J Physiol Pharmacol 2020 Mar 20;98(3):156-161. Epub 2020 Feb 20.

Department of Anaesthesiology, Xinjiang Medical University, Affiliated Tumour Hospital, Xinjiang, PR China.

Hyperglycemia mediates oxidative stress, thus inducing transcription factor nuclear factor kappa B (NF-κB) activation, increasing endothelial adhesion molecule expression and monocyte/endothelial interaction, and resulting in endothelial injury. Ketamine was reported to attenuate oxidative stress in many cases. In this research, we determined whether and how ketamine protects against high-glucose-mediated augmentation of monocyte/endothelial interaction and endothelial adhesion molecule expression in human umbilical vein endothelial cells. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0185DOI Listing

Inhibition of autophagy improves resistance and enhances sensitivity of gastric cancer cells to cisplatin.

Can J Physiol Pharmacol 2020 Feb 14. Epub 2020 Feb 14.

Zhengzhou University, 12636, School of Pharmaceutical Sciences, Zhengzhou, Henan, China.

Autophagy plays critical roles in tumorigenesis, while effects of autophagy on chemoresistance of cancer cells had great disparity. This study aims to explore the impacts of autophagy on the sensitivity and resistance of gastric cancer cells to cisplatin (DDP). We firstly demonstrated there was stronger autophagy activity in gastric cancer SGC-7901 cells than that in DDP-resisting SGC-7901/DDP cells. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0477DOI Listing
February 2020

Current trends in physiological sciences: from cell signals to the biology of aging.

Authors:

Can J Physiol Pharmacol 2020 Feb;98(2):iii

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http://dx.doi.org/10.1139/cjpp-2020-0059DOI Listing
February 2020

Scleraxis as a Prognostic Marker of Myocardial Fibrosis in Hypertrophic Cardiomyopathy (SPARC) Study.

Can J Physiol Pharmacol 2020 Feb 6. Epub 2020 Feb 6.

University of Manitoba Faculty of Health Sciences, 423134, Section of Cardiology, Department of Internal Medicine, Winnipeg, Manitoba, Canada ;

Interstitial fibrosis is a histopathological hallmark of hypertrophic cardiomyopathy (HCM). Although extracellular matrix (ECM) biomarkers, including matrix metalloproteinases, are overexpressed in HCM patients, they do not correlate with sudden cardiac death (SCD) risk. The objective of this study was to determine whether scleraxis, a transcription factor that regulates collagen gene expression, is detectable in HCM patients and correlates with disease burden. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0636DOI Listing
February 2020

Expression of cholinesterases and their anchoring proteins in rat heart.

Can J Physiol Pharmacol 2020 Feb 4. Epub 2020 Feb 4.

Comenius University in Bratislava Faculty of Pharmacy, 91876, Department of pharmacology and toxicology, Bratislava, Slovakia.

Acetylcholine-mediated vagal transmission as well as non-neuronal acetylcholine (ACh) release are considered cardioprotective in pathological situations with increased sympathetic drive such as ischemic-reperfusion and cardiac remodeling. ACh action is terminated by hydrolysis by the cholinesterases (ChEs), acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Both ChEs exist in multiple molecular variants either soluble or anchored by specific anchoring proteins like collagen Q anchoring protein (ColQ) and proline-rich membrane anchoring protein (PRiMA). Read More

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http://dx.doi.org/10.1139/cjpp-2019-0565DOI Listing
February 2020

Pharmacokinetic profile of the selective 5-HT3 receptor antagonist ondansetron in the rat: an original study and a mini-review of the behavioural pharmacological literature in the rat.

Can J Physiol Pharmacol 2020 Feb 4. Epub 2020 Feb 4.

Montreal Neurological Institute and Hospital, 55981, Montreal, Quebec, Canada ;

Selective pharmacological modulation of the 5-hydroxytryptamine (5-HT) type 3 (5-HT3) receptor has led to the development of therapeutics including ondansetron, a highly-selective 5-HT3 antagonist used as an anti-emetic for patients undergoing chemotherapy. Pre-clinical studies in rat have shown the effects of small doses of ondansetron on cognition, behavioural sensitisation and epilepsy. However, the pharmacokinetic (PK) profile of ondansetron in rat has not been described, which limits the translational relevance of these findings. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0551DOI Listing
February 2020

Metformin regulates TRPM6, a potential explanation for magnesium imbalance in type 2 diabetes patients.

Can J Physiol Pharmacol 2020 02 4. Epub 2020 Feb 4.

Radboudumc, 6034, Physiology , Radboud Institute for Molecular Life Sciences , Geert Grooteplein Zuid 28 , Nijmegen, Netherlands , 6525 GA ;

Metformin therapy is associated with lower serum magnesium (Mg2+) levels in type 2 diabetes (T2D) patients. The TRPM6 channel determines the fine-tuning of Mg2+ (re)absorption in intestine and kidney. Therefore, we aimed to investigate the short- and long-term effects of metformin on TRPM6. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0570DOI Listing
February 2020

Pharmacokinetics and tissue distribution study of herpetotriol in rats by ultrahigh-performance liquid chromatography with tandem mass spectrometry.

Can J Physiol Pharmacol 2020 Feb 3:366-372. Epub 2020 Feb 3.

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P.R. China.

Herpetotriol, a typical lignan in Wall's seeds that has long been used to treat icterhepatitis and indigestion and other related diseases in Tibet, is of potential hepatoprotection. This study aims to study the pharmacokinetics features of herpetotriol, including the blood drug concentration - time curve and tissue distribution. The ultrahigh-performance liquid chromatography with tandem mass spectrometry method was established to detect herpetotriol concentration in plasma and tissues, and the method showed good linearity from 10 to 2000 ng/mL ( ≥ 0. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0633DOI Listing
February 2020

Butyrate improves cardiac function and sympathetic neural remodeling following myocardial infarction in rats.

Can J Physiol Pharmacol 2020 Jan 30. Epub 2020 Jan 30.

Nanchang University, 47861, Department of Cardiology, The First Hospital of Nanchang,The Third Affiliated Hospital Of Nanchang University., Nanchang, Jiangxi, China ;

Increased inflammation is found in the cardiac sympathetic neural remodeling with malignant ventricular arrhythmia (VA) following myocardial infarction (MI). Butyrate, as a microbiota-derived short-chain fatty acids, can inhibit inflammation and myocardial hypertrophy. However, the role of butyrate in sympathetic neural remodeling after MI is unknown. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0531DOI Listing
January 2020

Elevated aldolase 1A, retrogene 1 expression induces cardiac apoptosis in rat experimental autoimmune myocarditis model.

Can J Physiol Pharmacol 2020 Jan 30:373-382. Epub 2020 Jan 30.

Department of Life Sciences, Korea University, Seoul 02841, Korea.

Acute myocarditis is an unpredictable heart disease that is caused by inflammation-associated cell death. Although viral infection and drug exposure are known to induce acute myocarditis, the molecular basis for its development remains undefined. Using proteomics and molecular analyses in myosin-induced rat experimental autoimmune myocarditis (EAM), we identified that elevated expression of aldolase 1A, retrogene 1 (Aldoart1) is critical to induce mitochondrial dysfunction and acute myocarditis development. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0539DOI Listing
January 2020

Morphometric analysis of structural renal alterations and beneficial effects of aminoguanidine in acute kidney injury induced by cisplatin in rats.

Can J Physiol Pharmacol 2020 Feb 29;98(2):117-123. Epub 2020 Jan 29.

University of Nis, Faculty of Medicine, Bulevar Dr. Zoran Djindjic 81, Nis, Serbia.

Since cisplatin-induced nephrotoxicity has very important clinical consequences, the purpose of this study was to determine the potential protective effect of aminoguanidine on the acute kidney injury caused by cisplatin. Experiments were done on 40 Wistar rats divided into four groups. The CIS group received cisplatin in a single dose of 8 mg/kg, while the CISAG group received the same dose of cisplatin and aminoguanidine (100 mg/kg) by intraperitoneal injections. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0252DOI Listing
February 2020

Meclizine ameliorates memory deficits in streptozotocin-induced experimental dementia in mice: role of nuclear pregnane X receptors.

Can J Physiol Pharmacol 2020 Jan 14:383-390. Epub 2020 Jan 14.

Department of Pharmacology, Chandigarh College of Pharmacy, Mohali (Punjab), India.

Pregnane X receptors (PXRs) regulate the expression of ATP-binding cassette proteins transporters and organic anion transporting polypeptides responsible for influx/efflux of xenobiotics across the brain. Ligand activation of PXR augments the expression of P-gp and promotes amyloid-β clearance across the blood-brain barrier. Dementia was induced in mice by intacerebroventricular administration of streptozotocin (STZ) followed by treatment with meclizine, a PXR agonist, and subsequently exposed to the Morris water maze test and biochemical and histopathological analysis to evaluate the effect on cognition. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0421DOI Listing
January 2020

Suppression of high-mobility group box 1 ameliorates xerostomia in a Sjögren syndrome-triggered mouse model.

Can J Physiol Pharmacol 2020 Jan 14:351-356. Epub 2020 Jan 14.

Department of Nephrology, Xijing Hospital, The First Affiliated Hospital of Fourth Military Medical University, Xi'an, Shaanxi 710032, People's Republic of China.

Xerostomia is a self-conscious symptom. High-mobility group box 1 (HMGB1) promotes pro-inflammatory effects in many diseases. This study aimed to clarify the role of HMGB1 in Sjögren syndrome (SS)-triggered xerostomia. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0337DOI Listing
January 2020

Rhein attenuates lipopolysaccharide-primed inflammation through NF-κB inhibition in RAW264.7 cells: targeting the PPAR-γ signal pathway.

Can J Physiol Pharmacol 2019 Dec 17:357-365. Epub 2019 Dec 17.

School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.

Inflammation is a common inducer of numerous severe diseases such as sepsis. The NF-κB signaling pathway plays a key role in the inflammatory process. Its activation promotes the release of pro-inflammatory mediators like inducible nitric oxide synthase and tumor necrosis factor alpha. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0389DOI Listing
December 2019

Stimulation of the endogenous hydrogen sulfide synthesis suppresses oxidative-nitrosative stress and restores endothelial-dependent vasorelaxation in old rats.

Can J Physiol Pharmacol 2020 May 17;98(5):275-281. Epub 2019 Dec 17.

Department of Blood Circulation, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine; Bogomoletz Institute of Physiology, 4, Bogomolets St., Kyiv, Ukraine, 01024.

Hydrogen sulfide (HS) is an endogenous gas transmitter with profound effects on the cardiovascular system. We hypothesized that stimulation of HS synthesis might alleviate age-associated changes in vascular reactivity. Pyridoxal-5-phosphate (PLP), the coenzyme of HS-synthesizing enzymes, was administrated to old male Wistar rats per os at a dose of 0. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0411DOI Listing

Upregulation of mitochondrial E3 ubiquitin ligase 1 in rat heart contributes to ischemia/reperfusion injury.

Can J Physiol Pharmacol 2020 May 11;98(5):259-266. Epub 2019 Dec 11.

Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.

Mitochondrial dysfunctions are responsible for myocardial injury upon ischemia/reperfusion (I/R), and mitochondrial E3 ubiquitin ligase 1 (Mul1) plays an important role in maintaining mitochondrial functions. This study aims to explore the function of Mul1 in myocardial I/R injury and the underlying mechanisms. The Sprague-Dawley rat hearts were subjected to 1 h of ischemia plus 3 h of reperfusion, which showed the I/R injury (increase in infarct size and creatine kinase release) and the elevated total and mitochondrial protein levels of Mul1 and p53 accompanied by the enhanced interactions between Mul1 and p53 as well as p53 and small a ubiquitin-like modifier (SUMO1). Read More

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http://dx.doi.org/10.1139/cjpp-2019-0285DOI Listing