9,114 results match your criteria Canadian journal of microbiology[Journal]


Delineating the origins of the multidrug-resistant (MDR) pathogens in ornamental fish farms by multilocus sequence typing and identification of a novel MDR plasmid.

Can J Microbiol 2019 Apr 9. Epub 2019 Apr 9.

Dalian Ocean University, 26480, Dalian, Liaoning, China ;

To evaluate the overuse of antibiotics and identify the origin of pathogens in the ornamental fish industry, a field investigation was conducted for three representative fish farms in Liaoning province, China. Drug-resistant pathogens in the fish pond and groundwater were isolated and sub-typed by multilocus sequence typing (MLST). In total, 33 pathogenic strains including Aeromonas veronii and other five pathogens were isolated from the diseased fish and groundwater. Read More

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http://dx.doi.org/10.1139/cjm-2019-0097DOI Listing

Bacterial community diversity associated with the severity of bacterial wilt disease in tomato fields in south-east China.

Can J Microbiol 2019 Apr 8. Epub 2019 Apr 8.

Xiamen University, School of Life Sciences, Xiamen, China ;

Tomato bacterial wilt caused by Ralstonia solanacearum is a devastating plant disease. The aim of this study was to investigate the relationship among soil nutrients, rhizobacterial community and quantification of R. solanacearum, and appropriate information for control the disease. Read More

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http://dx.doi.org/10.1139/cjm-2018-0637DOI Listing
April 2019
2 Reads

Bactéries sporulées et biofilms : un problème récurrent dans les lignes de production de lait reconstitué ou recombiné pasteurisé.

Authors:
Fadila Malek

Can J Microbiol 2019 Apr 1:1-16. Epub 2019 Apr 1.

Département de Biologie, Faculté SNV-STU, Université de Tlemcen, Tlemcen, 13000, Algérie.

In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is inefficient against bacterial spores. Read More

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http://dx.doi.org/10.1139/cjm-2018-0435DOI Listing
April 2019
7 Reads

Mudflat reclamation causes change in the composition of fungal communities under long-term rice cultivation.

Can J Microbiol 2019 Mar 29. Epub 2019 Mar 29.

College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu Province, PR China, Yangzhou, China ;

Fungi play a critical role in farmland ecosystems, especially in improving soil fertility; however, little is known about the changes in fungal communities caused by mudflat reclamation under rice cultivation. In this study, mudflats located in Yancheng, China, which were divided into nine plots with 0, 11, and 20 years of successive rice cultivation histories, were sampled to determine the fungal community composition by using Illumina MiSeq sequencing. Results show that the Shannon diversity of the fungal communities had no significant changes, while the species richness increased during mudflat reclamation with long-term rice cultivation. Read More

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http://dx.doi.org/10.1139/cjm-2019-0005DOI Listing

Reshaping fecal gut microbiota composition by growing with Polygonum cuspidatum, Houttuynia cordata Thunb, and Ipomoea aquatica Forsk.

Can J Microbiol 2019 Mar 28. Epub 2019 Mar 28.

Chinese Academy of Fishery Sciences Freshwater Fisheries Research Center, 273339, Wuxi, Jiangsu, China ;

We performed sequencing using samples cultivated in floating beds with different Chinese medicinal herbs to analyze the changes in the gut microbiota composition of tilapia feces. Fusobacteria (ranging from 49.0% to 73. Read More

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http://dx.doi.org/10.1139/cjm-2018-0497DOI Listing

Nitrogen fertilizer is a key factor effecting the soil chemical and microbial communities in a Mollisol.

Can J Microbiol 2019 Mar 22. Epub 2019 Mar 22.

School of Engineering, RMIT University, GPO Box 2476, Melbourne 3001, VIC, Australia, Melbourne, Australia ;

Microbial communities drive geochemical cycles in soils. Relatively few studies have assessed the long-term impacts of different types of soil amendments under field conditions in long-term experiments. The response of soil microbial organisms in a Mollisol cultivated with maize for 35 years was examined. Read More

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http://dx.doi.org/10.1139/cjm-2018-0683DOI Listing

Rapid identification of Erwinia amylovora and Pseudomonas syringae species and characterization of E. amylovora streptomycin resistance using quantitative PCR assays.

Can J Microbiol 2019 Mar 22. Epub 2019 Mar 22.

AAC-AAFC, Vineland, Ontario, Canada ;

Erwinia amylovora and Pseudomonas syringae are bacterial phytopathogens responsible for considerable yield losses in commercial pome fruit production. The pathogens, if left untreated, can compromise tree health and economically impact entire commercial fruit productions. Historically, the choice of effective control methods has been limited. Read More

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http://dx.doi.org/10.1139/cjm-2018-0587DOI Listing
March 2019
1 Read

Complete genome sequence of Paracoccus denitrificans ATCC 19367 and its denitrification characteristics.

Can J Microbiol 2019 Mar 21. Epub 2019 Mar 21.

Department of Food and Environmental Engineering, Yangjiang Polytechnic, Yangjiang, Canada ;

Studies show that Paracoccus denitrificans can denitrify under aerobic conditions. However, the lack of its genome sequence restricted molecular studies and practical applications. In this study, the complete genome of P. Read More

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http://dx.doi.org/10.1139/cjm-2019-0037DOI Listing
March 2019
2 Reads

Colonization and degradation of polyhydroxyalkanoates by lipase-producing bacteria.

Can J Microbiol 2019 Mar 21:1-15. Epub 2019 Mar 21.

a Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.

Biodegradation of short-chain-length polyhydroxyalkanoates (scl-PHAs) and medium-chain-length polyhydroxyalkanoates (mcl-PHAs) was studied using 2 bacteria, Pseudomonas chlororaphis and Acinetobacter lwoffii, which secrete an enzyme, or enzymes, with lipase activity. These bacteria produced clear zones of depolymerization on Petri plates containing colloidal solutions of PHA polymers with different monomer compositions. Lipase activity in these bacteria was measured using p-nitrophenyl octanoate as a substrate. Read More

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http://dx.doi.org/10.1139/cjm-2019-0042DOI Listing

Propidium monoazide real-time PCR amplification for viable Salmonella species and Salmonella Heidelberg in pork.

Can J Microbiol 2019 Mar 13:1-9. Epub 2019 Mar 13.

a College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China.

Salmonella enterica serovar Heidelberg causes foodborne infections and is a major threat to the food chain and public health. In this study, we aimed to develop a rapid molecular typing approach to identify Salmonella enterica serovar Heidelberg. Using comparative genomics, four serovar-specific gene fragments were identified, and a real-time polymerase chain reaction (PCR) combined with a propidium monoazide (PMA) pretreatment method was developed for simultaneous detection of viable Salmonella sp. Read More

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http://dx.doi.org/10.1139/cjm-2018-0547DOI Listing
March 2019
1 Read

The Campylobacter fetus S layer provides resistance to photoactivated zinc oxide nanoparticles.

Can J Microbiol 2019 Mar 13:1-11. Epub 2019 Mar 13.

Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada.

The antimicrobial activity of metal-based compounds, including metal oxides, has resulted in numerous agricultural, industrial, and medical applications. Zinc oxide nanoparticles are toxic to Gram-positive and Gram-negative bacteria as well as to some fungi. In this study we assess the sensitivity of Campylobacter fetus, a Gram-negative bacterial pathogen of humans and animals, to ZnO nanoparticles and determine whether the S layer protects C. Read More

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http://dx.doi.org/10.1139/cjm-2018-0451DOI Listing

Bacterial community succession in dairy manure composting with a static composting technique.

Can J Microbiol 2019 Mar 12:1-14. Epub 2019 Mar 12.

College of Resources and Environment, Northeast Agricultural University, Harbin 150030, P.R. China.

This study applied high-throughput sequencing technology and PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved state) to examine the microbial population dynamics during the composting of dairy manure and rice straw in a static (without turning) composting system. The results showed that the composition of the bacterial community varied significantly during the composting process. The dominant phyla included Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Chloroflexi. Read More

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http://dx.doi.org/10.1139/cjm-2018-0560DOI Listing
March 2019
11 Reads

Resistance to benzalkonium chloride and cadmium chloride in Listeria monocytogenes isolates from food and food-processing environments in southern Brazil.

Can J Microbiol 2019 Feb 5:1-7. Epub 2019 Feb 5.

a Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

Resistance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many Listeria monocytogenes strains worldwide and is associated with cadmium chloride (CC) resistance. Therefore, the aims of this study were to evaluate the susceptibility to BC and CC, detect the presence of resistance genes, and investigate the possible role of efflux pumps in BC and CC resistance in L. monocytogenes isolates from food and food-processing environments in southern Brazil. Read More

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http://dx.doi.org/10.1139/cjm-2018-0618DOI Listing
February 2019
1 Read

Development and application of an indirect enzyme-linked immunosorbent assay using recombinant S1 for serological testing of porcine epidemic diarrhea virus.

Can J Microbiol 2019 Feb 1:1-10. Epub 2019 Feb 1.

a Zhejiang Provincial Key Lab of Preventive Veterinary Medicine, Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China.

Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was expressed with the Bac-to-Bac system and purified by nickel-affinity chromatography. Read More

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http://dx.doi.org/10.1139/cjm-2018-0240DOI Listing
February 2019
3 Reads
1.221 Impact Factor

Bacillus subtilis SL-13 biochar formulation promotes pepper plant growth and soil improvement.

Can J Microbiol 2019 Jan 31:1-10. Epub 2019 Jan 31.

a School of Chemistry and Chemical Engineering, The Key Lab for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi 832003, P.R. China.

The use of microbial fertilizers can help to avoid the harmful effects of traditional agricultural fertilizers and pesticides; however, there are many constraints on the practical application of such fertilizers. In this study, microbial biochar formulations (MBFs) were obtained by loading biochar, created from agricultural waste, with Bacillus subtilis SL-13. The effects of the MBF on pepper plant growth and soil fertility were studied in pot experiments. Read More

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http://dx.doi.org/10.1139/cjm-2018-0333DOI Listing
January 2019
3 Reads

Evaluation of ACC-deaminase-producing rhizobacteria to alleviate water-stress impacts in wheat (Triticum aestivum L.) plants.

Can J Microbiol 2019 Jan 31:1-17. Epub 2019 Jan 31.

a Department of Biological Sciences, CBS&H, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, U.S. Nagar, Uttarakhand, India.

Application of plant-growth-promoting rhizobacteria (PGPR) is an environmentally sustainable option to reduce the effects of abiotic and biotic stresses on plant growth and productivity. Bacteria isolated from rain-fed agriculture field soils in the Central Himalaya Kumaun region, India, were evaluated for the production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Those producing ACC deaminase in high amounts were evaluated for their potential to improve wheat (Triticum aestivum L. Read More

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http://dx.doi.org/10.1139/cjm-2018-0636DOI Listing
January 2019

Genomic comparison of facultatively anaerobic and obligatory aerobic Caldibacillus debilis strains GB1 and Tf helps explain physiological differences.

Can J Microbiol 2019 Jan 29:1-8. Epub 2019 Jan 29.

a Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.

Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Read More

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http://dx.doi.org/10.1139/cjm-2018-0464DOI Listing
January 2019

Sulfur-oxidizing buffalo dung bacteria enhance growth and yield of Foeniculum vulgare Mill.

Can J Microbiol 2019 Jan 18:1-10. Epub 2019 Jan 18.

Department of Botany and Microbiology, Gurukula Kangri Vishwavidyalaya, Haridwar 249-404, Uttarakhand, India.

This study aimed to harness the benefits of sulfur-oxidizing beneficial bacteria from buffalo dung to improve crop yields of Foeniculum vulgare. A total of 61 bacterial isolates were screened from buffalo dung, of which 40 isolates exhibited plant-growth-promoting attributes, such as phosphate solubilization, indole-3-acetic acid production, and hydrogen cyanide production. Of these 40, four bacterial isolates, viz. Read More

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http://dx.doi.org/10.1139/cjm-2018-0476DOI Listing
January 2019
1.221 Impact Factor

Insight into the correlation between biochar amendment and shifts in bacterial community 4 years after a single incorporation in soybean- and maize-planted soils in northeastern China.

Can J Microbiol 2019 Jan 16:1-12. Epub 2019 Jan 16.

School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, P.R. China.

To date, there have been few reports examining the correlation between biochar treatments, crop species, and microbiome shifts. In this study, shifts in the soil bacterial community were investigated 4 years after a single incorporation of biochar in soils planted with soybeans and maize. Clear changes in the bacterial community composition and structure were detected in the soybean-planted soil amended with low-titer biochar (7. Read More

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http://dx.doi.org/10.1139/cjm-2018-0366DOI Listing
January 2019

Isolation and characterization of mercury-resistant bacteria from wastewater sources in Egypt.

Can J Microbiol 2019 Apr 11;65(4):308-321. Epub 2019 Jan 11.

c Department of Microbiology & Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia 11566, Cairo, Egypt.

An important mechanism for microbial resistance to mercury is its reduction into elemental mercury (facilitated by the merA gene). Thirty-eight microbial isolates from a variety of wastewater sources in Egypt were collected. Approximately 14 of the 38 isolates exhibited not only a high degree of tolerance to mercury (up to 160 ppm) but also a high degree of resistance to other tested heavy metals (Cu, Co, Ni, and Zn). Read More

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http://dx.doi.org/10.1139/cjm-2018-0379DOI Listing
April 2019
1 Read

Enhanced depolymerization and utilization of raw lignocellulosic material by co-cultures of Ruminiclostridium thermocellum with hemicellulose-utilizing partners.

Can J Microbiol 2019 Apr 4;65(4):296-307. Epub 2019 Jan 4.

Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.

Ruminiclostridium thermocellum is one of the most promising candidates for consolidated bioprocessing (CBP) of low-cost lignocellulosic materials to biofuels but it still shows poor performance in its ability to deconstruct untreated lignocellulosic substrates. One promising approach to increase R. thermocellum's rate of hydrolysis is to co-culture this cellulose-specialist with partners that possess synergistic hydrolysis enzymes and metabolic capabilities. Read More

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http://dx.doi.org/10.1139/cjm-2018-0535DOI Listing

Widespread detection of antibiotic-resistant bacteria from natural aquatic environments in southern Ontario.

Can J Microbiol 2019 Apr 20;65(4):322-331. Epub 2018 Dec 20.

a Department of Chemistry and Biology, Ryerson University, 350 Victoria Street, Toronto, ON M5B 2K3, Canada.

To elucidate how widespread antibiotic resistance is in the surface water environment, we studied the prevalence of antibiotic resistance bacteria at four locations in southern Ontario. We found that the percentage of bacteria resistant to the antibiotic tetracycline was higher at the river site, which flows through agricultural land, and lower at the lake sites. A total of 225 colonies were selected for further testing of antibiotic disc susceptibility to eight different antibiotics to calculate the multiple antibiotic resistance (MAR) score and the antibiotic resistance index for each site. Read More

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http://dx.doi.org/10.1139/cjm-2018-0286DOI Listing

Fungal pathogens pose a potential threat to animal and plant health in desertified and pika-burrowed alpine meadows on the Tibetan Plateau.

Can J Microbiol 2018 Dec 19:1-12. Epub 2018 Dec 19.

a Key Laboratory of Alpine Ecology, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, P.R. China.

Intact Tibetan meadows provide significant defense against soil-borne pathogen dispersal. However, dramatic meadow degradation has been observed due to climate change and pika damage, but their impacts on soil-borne pathogens are still unclear. With approximately 40% of the world's population living in Tibetan Plateau and its downstream watersheds, this lack of knowledge should be of great concern. Read More

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http://dx.doi.org/10.1139/cjm-2018-0338DOI Listing
December 2018
1 Read
1.221 Impact Factor

Biogeography and genotypic diversity of Metarhizium brunneum and Metarhizium robertsii in northwestern North American soils.

Can J Microbiol 2019 Apr 11;65(4):261-281. Epub 2018 Dec 11.

a Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.

The biogeography and genotype diversity of Metarhizium species in northwestern North American soils was examined; 20 ecoregions were sampled, including 58 agricultural and 80 natural habitat subsites, and areas that were glaciated during the Pleistocene epoch. One hundred and twenty-nine isolates of M. brunneum, 26 isolates of M. Read More

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http://dx.doi.org/10.1139/cjm-2018-0297DOI Listing

Presence of genes associated with adhesion, invasion, and toxin production in Campylobacter jejuni isolates and effect of temperature on their expression.

Can J Microbiol 2019 Apr 11;65(4):253-260. Epub 2018 Dec 11.

a Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

The aims of this study were to evaluate the presence of genes associated with adhesion (cadF), invasion (ciaB), and cytotoxin production (cdtA, cdtB, and cdtC) among Campylobacter jejuni isolates from a poultry slaughterhouse and to investigate the effect of different temperatures on the expression of these virulence-associated genes. A total of 88 C. jejuni isolates from cecum, liver, chicken carcasses, chilled water, and scalding water were submitted to PCR assay for detection of virulence genes. Read More

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http://dx.doi.org/10.1139/cjm-2018-0539DOI Listing
April 2019
1 Read

Remediation of deterioration in microbial structure in continuous Pinellia ternata cropping soil by crop rotation.

Can J Microbiol 2019 Apr 3;65(4):282-295. Epub 2018 Dec 3.

a College of Life Sciences, Northwest A & F University, Yangling 712100, P.R. China.

Pinellia ternata is a traditional Chinese herb that suffers from continuous cropping (CC), which significantly decreases both yield and quality. The influence of CC on the microbiome in P. ternata rhizosphere and the effects of remediation on microbiota by rotational cropping (CR) were assessed by Illumina high-throughput sequencing technology. Read More

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http://dx.doi.org/10.1139/cjm-2018-0409DOI Listing
April 2019
1 Read

Evaluating the diversity and composition of bacterial communities associated with Vachellia pachyceras - the only existing native tree species in the Kuwait desert.

Can J Microbiol 2019 Mar 29;65(3):235-251. Epub 2018 Nov 29.

a Desert Agriculture and Ecosystems Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat 13109, Kuwait.

We investigated the diversity and composition of bacterial communities in rhizospheric and non-rhizospheric bulk soils as well as root nodule bacterial communities of Vachellia pachyceras - the only native tree species existing in the Kuwait desert. Community fingerprinting comparisons and 16S rDNA sequence identifications were used for characterization of the bacterial population using specific primers. The bacterial characterization of soil samples revealed four major phyla: Acidobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0421
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http://dx.doi.org/10.1139/cjm-2018-0421DOI Listing
March 2019
6 Reads

Microbial degradation of low-density polyethylene and synthesis of polyhydroxyalkanoate polymers.

Can J Microbiol 2019 Mar 28;65(3):224-234. Epub 2018 Nov 28.

a Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.

We have characterized the ability of eight bacterial strains to utilize powdered low-density polyethylene (LDPE) plastic (untreated and without any additives) as a sole carbon source. Cell mass production on LDPE-containing medium after 21 days of incubation varied between 0.083 ± 0. Read More

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http://dx.doi.org/10.1139/cjm-2018-0335DOI Listing
March 2019
17 Reads

Pseudomonas species isolated from tobacco seed promote root growth and reduce lead contents in Nicotiana tobacum K326.

Can J Microbiol 2019 Mar 20;65(3):214-223. Epub 2018 Nov 20.

a College of Agronomy, College of Bioscience and Biotechnology, College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R. China.

Endophytic bacteria are generally helpful for plant growth and protection. We isolated from tobacco seeds three Pseudomonas strains (K03, Y04, and N05) that could produce siderophores, indole-3-acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase, fix nitrogen, dissolve phosphorus and potassium, and tolerate heavy metals. In pot experiments, the three isolated strains significantly promoted root growth and increased the root enzyme activity in Nicotiana tobacum K326. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0434
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http://dx.doi.org/10.1139/cjm-2018-0434DOI Listing
March 2019
18 Reads

Glucose-induced changes in the bacterial communities of mine tailings at different acidification stages.

Can J Microbiol 2019 Mar 19;65(3):201-213. Epub 2018 Nov 19.

a School of Resources and Environmental Engineering, Anhui University, Hefei, Anhui Province, P.R. China.

Ecological restoration technologies applied to tailings can influence the associated bacterial communities. However, it is unknown if the shifts in these bacterial communities are caused by increased organic carbon. Glucose-induced respiration and high-throughput sequencing were used to assess the microbial activity and bacterial communities, respectively. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2017-0782
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http://dx.doi.org/10.1139/cjm-2017-0782DOI Listing
March 2019
11 Reads
1.221 Impact Factor

Impact of artificial reefs on sediment bacterial structure and function in Bohai Bay.

Can J Microbiol 2019 Mar 9;65(3):191-200. Epub 2018 Nov 9.

a Department of Resources and Environment and Ecology Laboratory, Tianjin Bohai Sea Fisheries Research Institute, Tianjin 300457, P.R. China.

Artificial reefs have significantly altered ecological and environmental conditions compared with natural reefs, but how these changes affect sediment bacteria structure and function is unknown. Here, we compared the structure and function of the sediment bacterial community in the artificial reef area, the future artificial reef area, and the control area in Bohai Bay by 16S rRNA genes sequencing. Our results indicated that bacteria communities in the sediment were both taxonomically and functionally different between the reef area and control area. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0157
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http://dx.doi.org/10.1139/cjm-2018-0157DOI Listing
March 2019
6 Reads

Hydrogen cyanide produced by Pseudomonas chlororaphis O6 is a key aphicidal metabolite.

Can J Microbiol 2019 Mar 6;65(3):185-190. Epub 2018 Nov 6.

a Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, South Korea.

A biocontrol bacterium, Pseudomonas chlororaphis O6 promotes plant health through multifaceted mechanisms. In this study, we used P. chlororaphis O6 mutants to examine metabolites with aphicidal activity. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0372
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http://dx.doi.org/10.1139/cjm-2018-0372DOI Listing
March 2019
15 Reads

Anti-adhesion of probiotic Enterococcus faecium WEFA23 against five pathogens and the beneficial effect of its S-layer proteins against Listeria monocytogenes.

Can J Microbiol 2019 Mar 5;65(3):175-184. Epub 2018 Nov 5.

a State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330031, P.R. China.

Enterococcus faecium WEFA23 is a potential probiotic strain isolated from Chinese infant feces. In this study, the antagonistic activity of E. faecium WEFA23 on adhesion to pathogens was investigated. Read More

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http://dx.doi.org/10.1139/cjm-2018-0031DOI Listing
March 2019
7 Reads

A real-time PCR regimen for testing environmental samples for Salmonella enterica subsp. enterica serovars of concern to the poultry industry, with special focus on Salmonella Enteritidis.

Can J Microbiol 2019 Feb 5;65(2):162-173. Epub 2018 Nov 5.

Animal Health Microbiology, Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada.

A real-time PCR (qPCR) regimen, using up to six genetic targets, was developed to rapidly detect Salmonella and in particular identify Salmonella Enteritidis. The test regimen was first evaluated using a reference culture collection of Salmonella to confirm the appropriateness of the selected targets, which included up to three genetic markers for discrimination of Salmonella Enteritidis from other Salmonella serovars commonly found in poultry facilities. The qPCR procedure was then compared with culture methods used to detect Salmonella using a collection of enrichment broths previously generated from 239 environmental samples collected from a large number of hatchery facilities across Canada over several years. Read More

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http://dx.doi.org/10.1139/cjm-2018-0417DOI Listing
February 2019

Identification of Borrelia bissettii in Ixodes scapularis ticks from New Brunswick, Canada.

Can J Microbiol 2019 Feb 5;65(2):155-161. Epub 2018 Nov 5.

Department of Biology, Mount Allison University, Sackville, NB E4L 1G7, Canada.

Lyme disease is a tick-borne disease that is emerging in Canada. The disease is caused by spirochetes of the Lyme borreliosis group, which is expanding as new species are discovered. In Canada, Lyme disease risk has so far been assessed primarily by detection of Borrelia burgdorferi sensu stricto. Read More

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http://dx.doi.org/10.1139/cjm-2018-0376DOI Listing
February 2019
2 Reads

Appointment of a new Deputy Executive Editor-in-Chief.

Authors:

Can J Microbiol 2018 11;64(11):iii

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http://dx.doi.org/10.1139/cjm-2018-deeicDOI Listing
November 2018

Capacity to adhere to and invade human epithelial cells, as related to the presence of virulence genes in, motility of, and biofilm formation of Campylobacter jejuni strains isolated from chicken and cattle.

Can J Microbiol 2019 Feb 19;65(2):126-134. Epub 2018 Oct 19.

b Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago, Chile.

Campylobacter jejuni is a zoonotic pathogen transmitted through the "farm to fork" route. Outbreaks are generally associated with the consumption of chicken meat; however, dairy cows, birds, wild and domestic food animals, and pets are other important sources. Currently, there are not enough data comparing the virulence of strains isolated from these reservoirs. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0503
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http://dx.doi.org/10.1139/cjm-2018-0503DOI Listing
February 2019
13 Reads

Analysis of carbohydrate-active enzymes in Thermogemmatispora sp. strain T81 reveals carbohydrate degradation ability.

Can J Microbiol 2018 Dec 19;64(12):992-1003. Epub 2018 Oct 19.

b Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.

The phylum Chloroflexi is phylogenetically diverse and is a deeply branching lineage of bacteria that express a broad spectrum of physiological and metabolic capabilities. Members of the order Ktedonobacteriales, including the families Ktedonobacteriaceae, Thermosporotrichaceae, and Thermogemmatisporaceae, all have flexible aerobic metabolisms capable of utilizing a wide range of carbohydrates. A number of species within these families are considered cellulolytic and are capable of using cellulose as a sole carbon and energy source. Read More

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http://dx.doi.org/10.1139/cjm-2018-0336DOI Listing
December 2018
99 Reads
1.221 Impact Factor

Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage.

Can J Microbiol 2019 Feb 18;65(2):135-143. Epub 2018 Oct 18.

a Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, North-12, West-5, Kita-ku, Sapporo 060-0812, Japan.

The obligate intracellular bacterium Chlamydia trachomatis activates the host cell apoptosis pathway at a late stage of its developmental cycle. However, whether caspase-3, which is a key enzyme of apoptosis, is activated in Chlamydia-infected cells remains unknown. Here, we established HEp-2 cells stably expressing cFluc-DEVD, which is a caspase-3 substrate sequence inserted into cyclic firefly luciferase, and then monitored the dynamics of caspase-3 activity in cells infected with Chlamydia. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0408
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http://dx.doi.org/10.1139/cjm-2018-0408DOI Listing
February 2019
7 Reads

Restoration of polar-flagellum motility and biofilm-forming capacity in the mmsB1 mutant of the alphaproteobacterium Azospirillum brasilense Sp245 points to a new role for a homologue of 3-hydroxyisobutyrate dehydrogenase.

Can J Microbiol 2019 Feb 18;65(2):144-154. Epub 2018 Oct 18.

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov, 13, 410049 Saratov, Russia.

The bacterium Azospirillum brasilense can swim and swarm owing to the rotation of a constitutive polar flagellum (Fla) and inducible lateral flagella, respectively. They also form biofilms on various interfaces. Experimental data on flagellar assembly and social behaviours in these bacteria are scarce. Read More

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http://dx.doi.org/10.1139/cjm-2018-0481DOI Listing
February 2019
1 Read

Genetic diversity and symbiotic efficiency difference of endophytic rhizobia of Medicago sativa.

Can J Microbiol 2019 Jan 1;65(1):68-83. Epub 2018 Oct 1.

a College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, P.R. China.

Research on rhizobium diversity has paved the way for diversification of rhizobial germplasm resources. Seventy-three endophytic bacterial isolates were collected from seven tissues of five alfalfa cultivars in three geographic locations in Gansu, China. Restriction fragment length polymorphism (RFLP) fingerprinting of 16S rRNA and analysis of concatenated sequence of three housekeeping genes (atpD, glnII, and recA) and two symbiotic genes (nodC and nifH) were used for strain identification. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0158
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http://dx.doi.org/10.1139/cjm-2018-0158DOI Listing
January 2019
14 Reads

Developmental stage has a greater effect than Cry1Ac expression in transgenic cotton on the phyllosphere mycobiome.

Can J Microbiol 2019 Feb 28;65(2):116-125. Epub 2018 Sep 28.

b Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.

Transgenic Bt cotton is widely cultivated, yet its impact on the phyllosphere mycobiome is poorly understood. Hence, the objective of this study was to investigate the effects resulting from the planting of Bt cotton on fungal diversity composition. The α diversity for the Bt cotton line SGK321 was lower than that of control plants at the budding stage and the blossoming and boll-forming stage, while an obvious increase in diversity for Bt cotton XP188 was observed at the same stage. Read More

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http://dx.doi.org/10.1139/cjm-2018-0309DOI Listing
February 2019
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The synergistic antifungal effects of sodium phenylbutyrate combined with azoles against Candida albicans via the regulation of the Ras-cAMP-PKA signalling pathway and virulence.

Can J Microbiol 2019 Feb 27;65(2):105-115. Epub 2018 Sep 27.

d Department of Pharmacy, Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong Province, P.R. China.

The pathogenic fungus Candida albicans is one of the most commonly clinically isolated fungal species, and its resistance to the antifungal drug fluconazole is known to be increasing. In this paper, we sought to characterize the effect of sodium phenylbutyrate used alone or in combination with azoles against resistant C. albicans. Read More

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http://dx.doi.org/10.1139/cjm-2018-0337DOI Listing
February 2019
2 Reads

Horizontal transfer of antibiotic resistance genes in clinical environments.

Can J Microbiol 2019 Jan 24;65(1):34-44. Epub 2018 Sep 24.

a Department of Biology, University of Regina, Regina, SK S4S 0A2, Canada.

A global medical crisis is unfolding as antibiotics lose effectiveness against a growing number of bacterial pathogens. Horizontal gene transfer (HGT) contributes significantly to the rapid spread of resistance, yet the transmission dynamics of genes that confer antibiotic resistance are poorly understood. Multiple mechanisms of HGT liberate genes from normal vertical inheritance. Read More

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http://dx.doi.org/10.1139/cjm-2018-0275DOI Listing
January 2019
1 Read

Comparing the effect of intestinal bacteria from rabbit, pig, and chicken on inflammatory response in cultured rabbit crypt and villus.

Can J Microbiol 2019 Jan 19;65(1):59-67. Epub 2018 Sep 19.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.

Rabbit is susceptible to intestinal infection, which often results in severe inflammatory response. To investigate whether the special community structure of rabbit intestinal bacteria contributes to this susceptibility, we compared the inflammatory responses of isolated rabbit crypt and villus to heat-treated total bacteria in pig, chicken, and rabbit ileal contents. The dominant phylum in pig and chicken ileum was Firmicutes, while Bacteroidetes was dominant in rabbit ileum. Read More

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http://dx.doi.org/10.1139/cjm-2017-0757DOI Listing
January 2019

Characterization and sequence analysis of potential biofertilizer and biocontrol agent Bacillus subtilis strain SEM-9 from silkworm excrement.

Can J Microbiol 2019 Jan 19;65(1):45-58. Epub 2018 Sep 19.

a The Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, P.R. China.

Fusarium wilt is a devastating soil-borne disease caused mainly by highly host-specific formae speciales of Fusarium oxysporum. Antagonistic microorganisms play a very important role in Fusarium wilt control, and the isolation of potential biocontrol strains is becoming more and more important. We isolated a bacterial strain (SEM-9) from the high-temperature stage of silkworm excrement composting, which had a marked ability to solubilize phosphorus, promote the growth and increase the yield of the small Chinese cabbage, and which also exhibited considerable antagonistic effect towards Fusarium sambucinum and other fungi. Read More

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http://dx.doi.org/10.1139/cjm-2018-0350DOI Listing
January 2019
2 Reads

Sand bedding as a reservoir for Lactococcus garvieae dissemination in dairy farms.

Can J Microbiol 2019 Jan 18;65(1):84-89. Epub 2018 Sep 18.

a Department of Food, Environmental and Nutritional Sciences, Division of Food Microbiology and Bioprocesses, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy.

Lactococcus garvieae is now recognized as a species with clinical significance for human and veterinary medicine. The aim of this study was to evaluate the presence of this pathogen in sand bedding and milk samples. Two farms in Minnesota with problems of clinical and subclinical mastitis due to streptococci-like organisms were selected. Read More

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http://dx.doi.org/10.1139/cjm-2018-0251DOI Listing
January 2019
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Engineering root microbiomes for healthier crops and soils using beneficial, environmentally safe bacteria.

Can J Microbiol 2019 Feb 18;65(2):91-104. Epub 2018 Sep 18.

b Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095-1606, USA.

The Green Revolution developed new crop varieties, which greatly improved food security worldwide. However, the growth of these plants relied heavily on chemical fertilizers and pesticides, which have led to an overuse of synthetic fertilizers, insecticides, and herbicides with serious environmental consequences and negative effects on human health. Environmentally friendly plant-growth-promoting methods to replace our current reliance on synthetic chemicals and to develop more sustainable agricultural practices to offset the damage caused by many agrochemicals are proposed herein. Read More

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http://dx.doi.org/10.1139/cjm-2018-0315DOI Listing
February 2019
5 Reads
1.221 Impact Factor

Deletion of oxyR in Legionella pneumophila causes growth defect on agar.

Can J Microbiol 2018 Dec 13;64(12):1030-1041. Epub 2018 Sep 13.

Department of Natural Resource Sciences, McGill University, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada.

The intracellular pathogen Legionella pneumophila (Lp) is a strict aerobe, surviving and replicating in environments where it frequently encounters reactive oxygen species (ROS), such as the nutrient-poor water environment and its replicative niche inside host cells. In many proteobacteria, the LysR-type regulator OxyR controls the oxidative stress response; however, the importance of the OxyR homologue in Lp is still unclear. Therefore, we undertook the characterization of phenotypes associated with the deletion of oxyR in Lp. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0129
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http://dx.doi.org/10.1139/cjm-2018-0129DOI Listing
December 2018
12 Reads

Multidisciplinary approaches for studying rhizobium-legume symbioses.

Can J Microbiol 2019 Jan 11;65(1):1-33. Epub 2018 Sep 11.

a Department of Biology, University of Florence, Sesto Fiorentino, FI 50019, Italy.

The rhizobium-legume symbiosis is a major source of fixed nitrogen (ammonia) in the biosphere. The potential for this process to increase agricultural yield while reducing the reliance on nitrogen-based fertilizers has generated interest in understanding and manipulating this process. For decades, rhizobium research has benefited from the use of leading techniques from a very broad set of fields, including population genetics, molecular genetics, genomics, and systems biology. Read More

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http://dx.doi.org/10.1139/cjm-2018-0377DOI Listing
January 2019
2 Reads