9,210 results match your criteria Canadian journal of microbiology[Journal]


Bioprospection of native psychrotolerant plant growth-promoting rhizobacteria from the Peruvian Andean Plateau soils associated with Chenopodium quinoa.

Can J Microbiol 2020 Jun 23. Epub 2020 Jun 23.

Universidad Nacional Agraria, 187163, Biology, Lima, Peru ;

The Peruvian Andean Plateau is one of the main production areas for native varieties of Chenopodium quinoa. This region is exposed to abrupt decrease of environmental temperature that affects production of crops. In this scenario, Plant Growth-Promoting (PGP) bacteria that tolerate low temperatures could be used as organic biofertilizers for the agriculture in this region. Read More

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http://dx.doi.org/10.1139/cjm-2020-0036DOI Listing

A short overview of the CRISPR-Cas adaptation stage.

Can J Microbiol 2020 Jun 19. Epub 2020 Jun 19.

Université Laval, 4440, Biochimie, microbiologie et bio-informatique , Pavillon Vachon , Quebec, Quebec, Canada , G1V 0A6 ;

CRISPR research began over 30 years ago with the incidental discovery of an unusual nucleotide arrangement in the Escherichia coli genome. It took twenty years to find the main function of CRISPR-Cas systems as an adaptive defence mechanism against invading nucleic acids and our knowledge of their biology has been steadily increased ever since. In parallel, the number of applications derived from CRISPR-Cas systems has risen spectacularly. Read More

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http://dx.doi.org/10.1139/cjm-2020-0212DOI Listing

Correction: Microbial diversity and community structure in agricultural soils suffering from 4 years of Pb contamination.

Can J Microbiol 2020 Jul 19;66(7):456. Epub 2020 Jun 19.

College of Natural Resources and Environment, Northwest A&F University, Ministry of Agriculture Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, People's Republic of China.

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http://dx.doi.org/10.1139/cjm-2020-0242DOI Listing

Community structure and diversity of the microbiomes of two microhabitats at the root-soil interface: implications of meta-analysis of the root-zone soil and root endosphere microbial communities in Xiong'an New Area.

Can J Microbiol 2020 Jun 11. Epub 2020 Jun 11.

Hebei Normal University, 66447, Shijiazhuang, China , 050024 ;

The diversity of the microbial compositions of the root-zone soil (the rhizosphere-surrounding soil) and root endosphere (all inner root tissues) of the two plant genera Pinus tabulaeformis Carr. and Ginkgo biloba L. were evaluated in Xiong'an New Area using high-throughput sequencing, as well as the influence of the soil edaphic parameters on microbial community compositions. Read More

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http://dx.doi.org/10.1139/cjm-2020-0061DOI Listing

Variety features differentiate microbiota in the grape leaves.

Can J Microbiol 2020 Jun 8. Epub 2020 Jun 8.

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Shuangqing Road 18# Haidian Distract , Beijing, China , 100085 ;

The dependence of plant health and crop quality on the epiphytic microbial community has been universally addressed, but little is known about plant-associated microbial communities under natural conditions. In this study, the bacterial and fungal communities on grape leaves were analyzed by bacterial 16S rRNA gene and fungal internal transcribed spacer (ITS) high-throughput sequencing. The results showed differences in the composition of microbial communities among leaf samples of nine varieties of wine grapes. Read More

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http://dx.doi.org/10.1139/cjm-2019-0551DOI Listing

and population dynamics and quantification of spores after inoculation on ornamental plants.

Can J Microbiol 2020 Jun 4:1-6. Epub 2020 Jun 4.

Centre SÈVE, Département de biologie, Faculté des sciences, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, QC J1K 2R1, Canada.

and are used in organic agriculture as an alternative to chemical pesticides to fight against phytopathogen organisms. These Gram-positive soil-dwelling bacteria are able to resist harsh conditions and survive by differentiating into endospores. Few studies have examined how bacterial populations change on plants over time, and whether they remain active or enter a dormant state. Read More

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http://dx.doi.org/10.1139/cjm-2020-0174DOI Listing

Occurrence of strains on a polychrome cotton painting and their elimination by anoxic treatment.

Can J Microbiol 2020 Jun 4:1-7. Epub 2020 Jun 4.

Department of Microbiology, Institute of Biological Science, Federal University of Minas Gerais, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte, CEP 31270-901, Minas Gerais, Brazil.

This study aimed to isolate and identify the population of filamentous fungi colonizing a cotton painting, whose conservation status was compromised and showed signs of biodeterioration due to dirt accumulation and microbial metabolism. In addition, microbiological techniques such as cultivation-dependent approach and molecular biology were used to identify microbial populations and to eliminate their metabolic action. For this, the nondestructive anoxic atmosphere technique was used, in which the microbial metabolism was affected by the absence of oxygen. Read More

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http://dx.doi.org/10.1139/cjm-2020-0173DOI Listing

Volatile organic compounds kill the white-nose syndrome fungus, , in hibernaculum sediment.

Can J Microbiol 2020 Jun 2:1-7. Epub 2020 Jun 2.

Department of Biology, Nesbitt Building, Carleton University, 1125 Colonel By Drive, Ottawa, ON K1S 5B6, Canada.

, the fungal pathogen that causes white-nose syndrome, has killed millions of bats across eastern North America and continues to threaten new bat populations. The spread and persistence of has likely been worsened by the ability of this fungus to grow as a saprotroph in the hibernaculum environment. Reducing the environmental growth of may improve bat survival. Read More

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http://dx.doi.org/10.1139/cjm-2020-0071DOI Listing

Correction: Isolation and characterization of bacteriophages from India, with lytic activity against .

Can J Microbiol 2020 Jul 29;66(7):455. Epub 2020 May 29.

Department of Biomedical Science, Acharya Narendra Dev College (University of Delhi) Govindpuri, Kalkaji, New Delhi 110019, India.

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http://dx.doi.org/10.1139/cjm-2020-0104DOI Listing
July 2020
1.221 Impact Factor

Proteomic analysis of the inhibitory effect of the butanolic fraction of on .

Can J Microbiol 2020 May 14:1-14. Epub 2020 May 14.

CONACYT-Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Colonia La Victoria, C.P. 83304, Hermosillo, Sonora, México.

, a plant native to northwestern Mexico, has an inhibitory effect against phytopathogenic fungi. Previous studies have shown that the butanolic extract of causes retardation and atrophy in mycelial growth of . However, the action mechanism of this extract is unknown. Read More

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http://dx.doi.org/10.1139/cjm-2020-0127DOI Listing

It takes three to tango: the importance of microbes, host plant, and soil management to elucidate manipulation strategies for the plant microbiome.

Can J Microbiol 2020 Jul 12;66(7):413-433. Epub 2020 May 12.

School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.

The world's population is expected to grow to almost 10 billion by 2050, placing unprecedented demands on agriculture and natural resources. The risk in food security is also aggravated by climate change and land degradation, which compromise agricultural productivity. In recent years, our understanding of the role of microbial communities on ecosystem functioning, including plant-associated microbes, has advanced considerably. Read More

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http://dx.doi.org/10.1139/cjm-2020-0085DOI Listing

Structural analysis of the lipopolysaccharide O-antigen from strain CC 7/3 JVN3 C1 and development of a mouse monoclonal antibody specific to the O-antigen.

Can J Microbiol 2020 May 12:1-6. Epub 2020 May 12.

Vaccine and Emerging Infections Research, Human Health Therapeutics Research Centre, National Research Council, Ottawa, ON K1A 0R6, Canada.

is becoming increasingly recognised as an emerging pathogen, gaining attention as a potential factor for exacerbating colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. Little is known about the virulence factors of this organism; thus, we have initiated studies to examine the bacterium's surface glycochemistry. In an effort to characterise the surface carbohydrates of , the aims of this study were to investigate the structure of the lipopolysaccharide (LPS) O-antigen of the cancer-associated isolate strain CC 7/3 JVN3 C1 (hereafter C1) and to develop monoclonal antibodies (mAbs) to the LPS O-antigen that may be beneficial to the growing field of research. Read More

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http://dx.doi.org/10.1139/cjm-2020-0117DOI Listing

Biological characteristics and whole-genome analysis of the phage PEf771.

Can J Microbiol 2020 May 6:1-16. Epub 2020 May 6.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China.

is a common pathogen causing refractory periapical periodontitis and secondary intraradicular infections. In this study, YN771 isolated from a re-treated root canal at a stomatology department was used as the host bacterium and was co-cultured with wastewater from the same department and patient samples to isolate a phage that lyses . We studied the biological and genomic characteristics of this phage. Read More

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http://dx.doi.org/10.1139/cjm-2019-0336DOI Listing

Administration of HS-08 increases intestinal acetate and induces immunoglobulin A secretion in mice.

Can J Microbiol 2020 Apr 29:1-10. Epub 2020 Apr 29.

Hokukon Co., Ltd., 66-20-2 Imaichi, Fukui 918-8152, Japan.

A probiotic is considered a live microbial feed supplement that has beneficial effects on the host. In this study, the probiotic property by which HS-08 strengthens the immune system was investigated. Using a murine model, we evaluated the abilities of this strain to increase intestinal short-chain fatty acid contents and to induce the production of mucosal immunoglobulin A (IgA), which are crucial for mucosal immune systems. Read More

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http://dx.doi.org/10.1139/cjm-2020-0020DOI Listing

Organic acids and root exudates of : effects on chemotaxis and biofilm formation of endophytic bacteria.

Can J Microbiol 2020 Apr 29:1-14. Epub 2020 Apr 29.

Department of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, 21 111 Lakeshore Drive, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.

Root colonization by plant-growth-promoting bacteria could not be useful without the beneficial properties of the bacterium itself. Thus, it is necessary to evaluate the bacterial capacity to form biofilms and establish a successful interaction with the plant roots. We assessed the ability of growth-promoting bacterial strains to form biofilm and display chemotactic behaviour in response to organic acids and (or) root exudates of the model plant This assessment was based on the evaluation of single strains of bacteria and a multispecies consortium. Read More

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http://dx.doi.org/10.1139/cjm-2020-0041DOI Listing

Impact of liquid hog manure applications on antibiotic resistance genes concentration in soil and drainage water in field crops.

Can J Microbiol 2020 Apr 24:1-13. Epub 2020 Apr 24.

Research and Development Institute for the Agri-environment, 335 Vingt-Cinq East Road, Saint-Bruno-de-Montarville, QC J3V 0G7, Canada.

Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteria in soil and drainage water under wheat and grain corn crops. Targeted ARGs (T), , and in liquid hog manure, soil, and water samples were quantified by qPCR. Read More

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http://dx.doi.org/10.1139/cjm-2019-0343DOI Listing

The yeast, the antifungal, and the wardrobe: a journey into antifungal resistance mechanisms of .

Can J Microbiol 2020 Jun 22;66(6):377-388. Epub 2020 Apr 22.

Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Graduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil.

is a prominent non- species involved in cases of candidemia, mainly causing infections in patients in intensive care units and (or) those presenting neutropenia. In recent years, several studies have reported an increase in the recovery rates of azole-resistant isolates. Understanding resistance is of great importance, since resistant strains are implicated in persistent or recurrent and breakthrough infections. Read More

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http://dx.doi.org/10.1139/cjm-2019-0531DOI Listing

Codon usage bias affects α-amylase mRNA level by altering RNA stability and cytosine methylation patterns in .

Can J Microbiol 2020 Apr 7:1-8. Epub 2020 Apr 7.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China.

Codon usage bias exists in almost every organism and is reported to regulate protein translation efficiency and folding. Besides translation, the preliminary role of codon usage bias on gene transcription has also been revealed in some eukaryotes such as . In this study, we took as an example the α-amylase-coding gene () and examined the role of codon usage bias in regulating gene expression in the typical prokaryote . Read More

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http://dx.doi.org/10.1139/cjm-2019-0624DOI Listing

Co-occurrence patterns among prokaryotes across an age gradient in pit mud of Chinese strong-flavor liquor.

Can J Microbiol 2020 Apr 1:1-10. Epub 2020 Apr 1.

School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan Province, People's Republic of China.

It is widely believed that the quality and characteristics of Chinese strong-flavor liquor (CSFL) are closely related to the age of the pit mud; CSFL produced from older pit mud tastes better. This study aimed to investigate the alteration and interaction of prokaryotic communities across an age gradient in pit mud. Prokaryotic microbes in different-aged pit mud (1, 6, and 10 years old) were analyzed by Illumina MiSeq sequencing of the 16S rRNA gene. Read More

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http://dx.doi.org/10.1139/cjm-2020-0012DOI Listing

Effects of bioporous carriers on the performance and microbial community structure in side-stream anaerobic membrane bioreactors.

Can J Microbiol 2020 Mar 30:1-15. Epub 2020 Mar 30.

School of Civil Engineering, Sichuan University of Science & Engineering, Zigong 643000, P.R. China.

The aim of this study was to investigate the effects of a volcanic rock porous carrier (VRPC) on sludge reduction, pollutant removal, and microbial community structure in an anaerobic side-stream reactor (ASSR). Three lab-scale membrane bioreactors (MBRs), including an anoxic-oxic MBR, which served as the control (C-MBR), an ASSR-coupled MBR (A-MBR), and an A-MBR filled with VRPC (FA-MBR) were stably and simultaneously operated for 120 days. The effect of the three reactors on the removal of chemical oxygen demand (COD) was almost negligible (all greater than 95%), but the average removal efficiency of ammonium nitrogen, total nitrogen, and total phosphorus was significantly improved by the insertion of an ASSR, especially when the ASSR was filled with VRPC. Read More

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http://dx.doi.org/10.1139/cjm-2019-0632DOI Listing
March 2020
1.221 Impact Factor

Cytosolic mtDNA released from pneumolysin-damaged mitochondria triggers IFN-β production in epithelial cells.

Can J Microbiol 2020 Jul 19;66(7):435-445. Epub 2020 Mar 19.

School of Laboratory Medicine, Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education, Chongqing Medical University, Chongqing 400016, People's Republic of China.

Pneumolysin (Ply) is a major virulence factor of . Ply-induced interferon-β (IFN-β) expression in host macrophages has been shown to be due to the accumulation of mitochondrial deoxyribonucleic acid (mtDNA) in the cytoplasm during infection. Our findings extend this work to show human bronchial epithelial cells that reside at the interface of inflammatory injury, BEAS-2B, adapt to local cues by altering mitochondrial states and releasing excess mtDNA. Read More

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http://dx.doi.org/10.1139/cjm-2019-0481DOI Listing

Isolation of strain SEM-2 from silkworm excrement and characterisation of its antagonistic effect against spp.

Can J Microbiol 2020 Jun 11;66(6):401-412. Epub 2020 Mar 11.

The Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, P.R. China.

wilt is a devastating soil-borne disease mainly caused by highly host-specific formae speciales of spp. Antagonistic microorganisms play a very important role in wilt control. Isolation of potential biocontrol strains has become increasingly important. Read More

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http://dx.doi.org/10.1139/cjm-2019-0621DOI Listing

Corn microbial diversity and its relationship to yield.

Can J Microbiol 2020 Mar 10:1-17. Epub 2020 Mar 10.

A&L Biologicals, Agroecological Research Services Centre, 2136 Jetstream Road, London, ON N5V 3P5, Canada.

This study aimed to identify possible relationships between corn ( L.) productivity and its endosphere microbial community. Any insights would be used to develop testable hypotheses at the farm level. Read More

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http://dx.doi.org/10.1139/cjm-2020-0002DOI Listing

Towards safer stable isotope probing - effect of formamide on the separation of isotope-labeled and unlabeled RNA by isopycnic density ultracentrifugation.

Can J Microbiol 2020 Mar 5:1-4. Epub 2020 Mar 5.

Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology and Hygiene Group, Furtwangen University, Villingen-Schwenningen, Germany.

RNA-based stable isotope probing (RNA-SIP) is used in molecular microbial ecology to link the identity of microorganisms in a complex community with the assimilation of a distinct substrate. The technique is highly dependent on a reliable separation of isotopic-labeled RNA from unlabeled RNA by isopycnic density gradient ultracentrifugation. Here we show that C-labeled and unlabeled RNA can be sufficiently separated by isopycnic ultracentrifugation even in the absence of formamide. Read More

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http://dx.doi.org/10.1139/cjm-2019-0612DOI Listing

The complex multicellular morphology of the food spoilage bacteria strains isolated from ground chicken.

Can J Microbiol 2020 Apr 2;66(4):303-312. Epub 2020 Mar 2.

US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center Molecular Characterization of Foodborne Pathogens Research Unit, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.

Herein we describe a highly structured, filamentous growth phenotype displayed by an isolate of the food spoilage microorganism . The growth morphology of this strain (strain BII) was dependent on environmental factors such as the growth media, incubation temperatures, and the inoculum concentration. Inoculation of cultures in highly dilute suspensions resulted in the formation of isolated, tight aggregates resembling fungal growth in liquid media. Read More

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http://dx.doi.org/10.1139/cjm-2019-0502DOI Listing

In Memoriam / In Memoriam.

Can J Microbiol 2020 Mar;66(3):iii-iv

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http://dx.doi.org/10.1139/cjm-2020-0073DOI Listing

Close-up on a bacterial informational war in the geocaulosphere.

Can J Microbiol 2020 Jul 24;66(7):447-454. Epub 2020 Feb 24.

Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM EA 4312) - Normandie Université - LMSM, 55 rue Saint-Germain, 27000 Evreux, France & Structure Fédérative de Recherche Normandie Végétale 4277 (NORVEGE).

The geocaulosphere is home to microbes that establish communication between themselves and others that disrupt them. These cell-to-cell communication systems are based on the synthesis and perception of signaling molecules, of which the best known belong to the -acyl-homoserine lactone (AHL) family. Among indigenous bacteria, certain Gram-positive actinobacteria can sense AHLs produced by soft-rot Gram-negative phytopathogens and can degrade the quorum-sensing AHL signals to impair the expression of virulence factors. Read More

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http://dx.doi.org/10.1139/cjm-2019-0546DOI Listing

Antibacterial and antibiofilm effects of α-humulene against .

Can J Microbiol 2020 Jun 19;66(6):389-399. Epub 2020 Feb 19.

Department of Medical Sciences, College of Medical Sciences, Soonchunhyang University, Asan, Republic of Korea.

The rapid increase in antibiotic resistance has prompted the discovery of drugs that reduce antibiotic resistance or new drugs that are an alternative to antibiotics. Plant extracts have health benefits and may also exhibit antibacterial and antibiofilm activities against pathogens. This study determined the antibacterial and antibiofilm effects of α-humulene extracted from plants against enterotoxigenic , which causes inflammatory bowel disease. Read More

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http://dx.doi.org/10.1139/cjm-2020-0004DOI Listing

Evaluating the risks associated with Shiga-toxin-producing (STEC) in private well waters in Canada.

Can J Microbiol 2020 May 18;66(5):337-350. Epub 2020 Feb 18.

Environmental Health Sciences, School of Public Health, University of Alberta, Edmonton, AB T6G 2G7, Canada.

Shiga-toxin-producing (STEC) represent a major concern for waterborne disease outbreaks associated with consumption of contaminated groundwater. Over 4 million people rely on private groundwater systems as their primary drinking water source in Canada; many of these systems do not meet current standards for water quality. This manuscript provides a scoping overview of studies examining STEC prevalence and occurrence in groundwater, and it includes a synopsis of the environmental variables affecting survival, transport, persistence, and overall occurrence of these important pathogenic microbes in private groundwater wells used for drinking purposes. Read More

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http://dx.doi.org/10.1139/cjm-2019-0329DOI Listing

Rhizospheric soil fungal community patterns of in response to altitude gradient in Yunnan, southwest China.

Can J Microbiol 2020 May 13;66(5):359-367. Epub 2020 Feb 13.

Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.

The magnitude of the impact of altitude gradient on microbial community and diversity has been studied in recent decades. Whereas bacteria have been the focus of most studies, fungi have been given relatively less attention. As a vital part of the macro- and microscopic ecosystem, rhizosphere fungi play a key role in organic matter decomposition and relative abundance of plant species and have an impact on plant growth and development. Read More

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http://dx.doi.org/10.1139/cjm-2019-0589DOI Listing

Preliminary screening of rhizobacteria for biocontrol of little seed canary grass ( Retz.) and wild oat ( L.) in wheat.

Can J Microbiol 2020 May 10;66(5):368-376. Epub 2020 Feb 10.

Department of Agronomy, University of Agriculture, Faisalabad 38040, Pakistan.

Conventional weed control methods often have environmental impact. The present study was conducted to screen selected accessions of for both potential biocontrol of and and potential concurrent growth promotion of wheat. The four strains (B11, T19, T24, and T75) were found positive for cyanide production, siderophore production, phosphorus solubilization, oxidase activity, catalase activity, and ACC deaminase activity in vitro. Read More

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http://dx.doi.org/10.1139/cjm-2019-0427DOI Listing

Insights into and gene regulation on the virulence of M5-90.

Can J Microbiol 2020 May 10;66(5):351-358. Epub 2020 Feb 10.

School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang 832000, P.R. China.

Iron is a fundamental element required by most organisms, including . Several researchers have suggested that the iron response regulator () and rhizobial iron regulator () genes regulate iron acquisition by , influencing heme synthesis by and virulence of this pathogen. However, little is known about another species, . Read More

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http://dx.doi.org/10.1139/cjm-2019-0393DOI Listing
May 2020
1.221 Impact Factor

Biofilm formation by South African non-O157 Shiga toxigenic on stainless steel coupons.

Can J Microbiol 2020 Apr 4;66(4):328-336. Epub 2020 Feb 4.

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.

This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 °C and after 168 h at 10 °C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on both the submerged surface and the air-liquid interface of coupons, whereas strains O116:H21, wzx-Onovel5:H19, O129:H21, and O26:H11 formed biofilm only at the air-liquid interface. Viable cell counts and scanning electron microscopy showed that biofilm formation increased ( < 0. Read More

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http://dx.doi.org/10.1139/cjm-2019-0554DOI Listing

biofertilizer application in a tea plantation reduces soil NO by changing denitrifier communities.

Can J Microbiol 2020 Mar 3;66(3):214-227. Epub 2020 Feb 3.

CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, P.R. China.

Increasing the use of nitrogen fertilizers in tea orchards has led to intense nitrous oxide (NO) emissions. Foliar application of biofertilizer has been proven to be beneficial for organic tea production. In this study, tea yield and quality were significantly improved after application of biofertilizer compared with the control but were not significantly different from chemical fertilizer treatments. Read More

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http://dx.doi.org/10.1139/cjm-2019-0511DOI Listing

Retraction: Analysis of antimicrobial silver nanoparticles synthesized by coastal strains of and .

Can J Microbiol 2020 02 30;66(2):169. Epub 2020 Jan 30.

Centre of advanced study in Marine Biology, Annamalai University, Parangipettai, 608502 Tamil Nadu, India.

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http://dx.doi.org/10.1139/cjm-2020-0029DOI Listing
February 2020

Biological H and CO oxidation activities are sensitive to compositional change of soil microbial communities.

Can J Microbiol 2020 Apr 30;66(4):263-273. Epub 2020 Jan 30.

Institut national de la recherche scientifique, Centre Armand Frappier Santé Biotechnologie, 531 boulevard des Prairies, Laval, QC H7V 1B7, Canada.

Trace gas uptake by microorganisms controls the oxidative capacity of the troposphere, but little is known about how this important function is affected by changes in soil microbial diversity. This article bridges that knowledge gap by examining the response of the microbial community-level physiological profiles (CLPPs), carbon dioxide (CO) production, and molecular hydrogen (H) and carbon monoxide (CO) oxidation activities to manipulation of microbial diversity in soil microcosms. Microbial diversity was manipulated by mixing nonsterile and sterile soil with and without the addition of antibiotics. Read More

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http://dx.doi.org/10.1139/cjm-2019-0412DOI Listing

Application of culturomics to characterize diverse anaerobic bacteria from the gastrointestinal tract of broiler chickens in relation to environmental reservoirs.

Can J Microbiol 2020 Apr 27;66(4):288-302. Epub 2020 Jan 27.

Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1, Canada.

Characterization of the microbiota of chickens is of current interest. The goals of the current study were to apply anaerobic isolation methods to comprehensively isolate and identify bacteria from the gastrointestinal tract of chickens and their environment. Bacterial communities within the drinking water were dominated by , whereas communities in litter were more representative of the cecum. Read More

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http://dx.doi.org/10.1139/cjm-2019-0469DOI Listing

FtsA G50E mutant suppresses the essential requirement for FtsK during bacterial cell division in .

Can J Microbiol 2020 Apr 23;66(4):313-327. Epub 2020 Jan 23.

Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada.

In , the N-terminal domain of the essential protein FtsK (FtsK) is proposed to modulate septum formation through the formation of dynamic and essential protein interactions with both the Z-ring and late-stage division machinery. Using genomic mutagenesis, complementation analysis, and pull-down assays, we aimed to identify protein interaction partners of FtsK essential to its function during division. Here, we identified the cytoplasmic Z-ring membrane anchoring protein FtsA as a direct protein-protein interaction partner of FtsK. Read More

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http://dx.doi.org/10.1139/cjm-2019-0493DOI Listing

Identification and characterization of the glycoside hydrolase family 18 genes from the entomopathogenic fungus genome.

Can J Microbiol 2020 Apr 21;66(4):274-287. Epub 2020 Jan 21.

School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.

Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the genome. Seventeen of the genes belonged to chitinases and one was an endo-β--acetylglucosaminidase (ENGase). Read More

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http://dx.doi.org/10.1139/cjm-2019-0129DOI Listing

Diversity and space-time dynamics of the bacterial communities in cotton () rhizosphere soil.

Can J Microbiol 2020 Mar 16;66(3):228-242. Epub 2020 Jan 16.

Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P.R. China.

Rhizosphere bacteria are key determinants of plant health and productivity. In this study, we used PCR-based next-generation sequencing to reveal the diversity and community composition of bacteria in the cotton rhizosphere from samples collected in Xinjiang Province, China. We identified 125 bacterial classes within 49 phyla from these samples. Read More

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http://dx.doi.org/10.1139/cjm-2019-0196DOI Listing

Production of prodigiosin pigment by is negatively associated with cellular ATP levels during high-rate, low-cell-density growth.

Can J Microbiol 2020 Mar 10;66(3):243-255. Epub 2020 Jan 10.

Charles T. Campbell Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, 203 Lothrop Street, Pittsburgh, PA 15213, USA.

is a facultatively anaerobic bacterium and the most recognized producer of the hydrophobic pigment prodigiosin. Previous work has shown that prodigiosin both increases ATP production during population lag phase and approximately doubles the stationary-phase cell yield. Here, we employed both batch and chemostat culture methods to investigate prodigiosin's role during high rate growth at low cell density as peak cellular ATP levels decline. Read More

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http://dx.doi.org/10.1139/cjm-2019-0548DOI Listing

Changes in polyhydroxyalkanoate granule accumulation make optical density measurement an unreliable method for estimating bacterial growth in .

Can J Microbiol 2020 Mar 9;66(3):256-262. Epub 2020 Jan 9.

Centre Armand-Frappier Santé Biotechnologie, Institut national de la recherche scientifique, Laval, Quebec, Canada.

Optical density (OD) measurement is the standard method used in microbiology for estimating bacterial concentrations in cultures. However, most studies do not compare these measurements with viable cell counts and assume that they reflect the real cell concentration. was recently identified as a polyhydroxyalkanoate (PHA) producer. Read More

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http://dx.doi.org/10.1139/cjm-2019-0342DOI Listing

detected on modified charcoal-cefoperazone-deoxycholate agar in a waste stabilization pond.

Can J Microbiol 2020 Mar 19;66(3):206-213. Epub 2019 Dec 19.

School of Environment and Science, Griffith University, Nathan, Queensland, Australia.

is a recommended reference pathogen for the verification and validation of water recycling schemes in Australia and globally. In a larger study investigating the efficacy of pathogen removal in waste stabilization ponds (WSP), we cultivated bacteria from wastewater samples on modified charcoal-cefoperazone-deoxycholate agar (mCCDA) targeting the growth of A high number of colonies characteristic of grew on this selective medium, but this did not correlate with qPCR data. Using primers targeting the 16S rRNA gene, and additional confirmatory tests to detect VS1, , , genes, we tested 80 random colonies from 10 WSP samples. Read More

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http://dx.doi.org/10.1139/cjm-2019-0282DOI Listing

Changes in structure and function of bacterial and fungal communities in open composting of Chinese herb residues.

Can J Microbiol 2020 Mar 2;66(3):194-205. Epub 2019 Dec 2.

Research Center for Metabolites, Shaanxi Institute of Microbiology, 76 Xiying Road, Xi'an, Shaanxi 710043, P.R. China.

In this study, dynamic changes in bacterial and fungal communities, metabolic characteristics, and trophic modes in Chinese herb residues open composting for 30 days were analyzed by using high-throughput sequencing, PICRUSt, and FUNGuild, respectively. and predominated at the early composting stage, while and became the dominant phyla during the active phase. Aerobic composting had a significant effect on bacterial metabolic characteristics and fungal trophic modes over the composting time. Read More

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http://dx.doi.org/10.1139/cjm-2019-0347DOI Listing
March 2020
1.221 Impact Factor

Effect of common bean seed exudates on growth, lipopolysaccharide production, and lipopolysaccharide transport gene expression of .

Can J Microbiol 2020 Mar 21;66(3):186-193. Epub 2019 Nov 21.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.

Lipopolysaccharide (LPS) is essential for successful nodulation during the symbiosis of rhizobia and legumes. However, the detailed mechanism of the LPS in this process has not yet been clearly elucidated. In this study, the effects of common bean seed exudates on the growth, lipopolysaccharide production, and lipopolysaccharide transport genes expression () of were investigated. Read More

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http://dx.doi.org/10.1139/cjm-2019-0413DOI Listing
March 2020
1.221 Impact Factor

Mode of action of nisin on .

Can J Microbiol 2020 Feb 19;66(2):161-168. Epub 2019 Nov 19.

Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON K1S 5B6, Canada.

Nisin is a class I polycyclic bacteriocin produced by the bacterium , which is used extensively as a food additive to inhibit the growth of foodborne Gram-positive bacteria. Nisin also inhibits growth of Gram-negative bacteria when combined with membrane-disrupting chelators such as citric acid. To gain insight into nisin's mode of action, we analyzed chemical-genetic interactions and identified nisin-sensitive strains in the Keio library of knockout mutants. Read More

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http://dx.doi.org/10.1139/cjm-2019-0315DOI Listing
February 2020
1.221 Impact Factor

Persistent contamination of , , , and at a broiler farm in New Zealand.

Can J Microbiol 2020 Mar 13;66(3):171-185. Epub 2019 Nov 13.

Department of Food Technology, School of Food and Advanced Technology, Massey University, Private Bag 102904, Auckland 0745, New Zealand.

Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of the pathogens , , , and was determined by standard methods at the farm level. After disinfection, swab samples collected from wall crevices, drinkers, and vents were heavily contaminated, as accumulated organic matter and dust likely protected the pathogens from the disinfectants used. Read More

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http://dx.doi.org/10.1139/cjm-2019-0280DOI Listing

Detection of canine parvovirus types 2b and 2c in canine faecal samples contaminating urban thoroughfares in Brazil.

Can J Microbiol 2020 Feb 12;66(2):138-143. Epub 2019 Nov 12.

Department of Veterinary Sciences, Federal University of Paraná, 85950-000, Palotina, PR, Brazil.

Canine parvovirus type 2 (CPV-2) is a highly contagious virus that causes acute gastroenteritis in dogs all over the world. Because of its stability in the environment, CPV-2 can remain infective for a long time, especially if protected in organic matter. To demonstrate CPV-2's potential as an environmental hazard for nonimmunized susceptible hosts, we investigated 50 faecal samples collected from public areas in a municipality of Paraná state, Brazil. Read More

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http://dx.doi.org/10.1139/cjm-2019-0137DOI Listing
February 2020

Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress.

Can J Microbiol 2020 Feb 12;66(2):144-160. Epub 2019 Nov 12.

Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh.

Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) from saline and non-saline areas in Bangladesh where electrical conductivity was measured as >7. Read More

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http://dx.doi.org/10.1139/cjm-2019-0323DOI Listing
February 2020

Antimicrobial capacity of ultrasound and ozone for enhancing bacterial safety on inoculated shredded green cabbage ( var. ).

Can J Microbiol 2020 Feb 7;66(2):125-137. Epub 2019 Nov 7.

Beijing Institute of Technology, Beijing 100081, P.R. China.

The high frequency and incidence of foodborne outbreaks related to fresh vegetables consumption is a major public health concern and an economic burden worldwide. This study evaluated the effect of individual and combined application of ultrasound (40 kHz, 100 W) and ozone on the inactivation of foodborne and , as well as their impact on cabbage color and vitamin C content. Plate count, scanning electron microscopy (SEM), and flow cytometry (FCM) following single or double staining with carboxyfluorescein diacetate and (or) propidium iodide were used to determine bacterial inactivation parameters, such as cell culturability, membrane integrity, intracellular enzyme activity, and injured and dead cells. Read More

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http://dx.doi.org/10.1139/cjm-2019-0313DOI Listing
February 2020
1.221 Impact Factor