1,940 results match your criteria Canadian Journal Of Veterinary Research-revue Canadienne De Recherche Veterinaire[Journal]


Genetic characterization of a hantavirus isolated from Heilongjiang province, China.

Can J Vet Res 2019 Jan;83(1):75-77

Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China (Wang, Cao, Hua); Heilongjiang International Travel Healthcare Center, Harbin, 150000, China (Cheng, Ju).

Hantavirus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). Heilongjiang Province is experiencing an epidemic of HFRS, the main causative agent is a variant of hantavirus called Seoul virus (SEOV). In this study, the entire genome of one SEOV, the DN2 strain, was sequenced and analyzed. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318823PMC
January 2019
6 Reads

Protective efficacy of an inactivated vaccine candidate lysed by GI24 against brucellosis in Korean black goats.

Can J Vet Res 2019 Jan;83(1):68-74

Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Gobong-ro 79, Iksan, Jeollabuk-do, Republic of Korea (Kim, Moon, Cho, Ochirkhuyag, Hur); Veterinary Histology, College of Veterinary Medicine, Chonbuk National University, Gobong-ro 79, Iksan, Jeollabuk-do, Republic of Korea (Akanda, Park).

The efficacy of GI24-lysed cells as a vaccine candidate against brucellosis in goats was evaluated on 2 groups of Korean black goats. Group A goats were immunized subcutaneously (SC) with sterile phosphate-buffered saline, whereas group B goats were immunized SC with approximately 3 × 10 lysed cells. Subcutaneous immunization with the lysed cells did not cause any negative impact on the overall clinical status, such as behavior and appetite, throughout the study period. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318821PMC
January 2019
1.022 Impact Factor

Comparison of 4 commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines against heterologous Korean PRRSV-1 and PRRSV-2 challenge.

Can J Vet Res 2019 Jan;83(1):57-67

Seoul National University, College of Veterinary Medicine, Department of Veterinary Pathology, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea.

The efficacy of 4 commercial porcine reproductive and respiratory syndrome virus (PRRSV) modified-live vaccines (MLV), against PRRSV-1 and PRRSV-2 was evaluated and compared in growing pigs. Two of the vaccines were based on PRRSV-1 and two on PRRSV-2. There were no significant differences between each of the two PRRSV-1 MLV vaccines and the two PRRSV-2 MLV vaccines respectively based on virology, immunological, and pathological evaluations. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318830PMC
January 2019
1 Read

Peripartum metabolic profiles in a Holstein dairy herd with alarm level prevalence of subclinical ketosis detected in early lactation.

Can J Vet Res 2019 Jan;83(1):50-56

Department of Veterinary Herd Health, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.

The aim of this study was to characterize peripartum metabolic profiles, including the insulin sensitivity index, in cows diagnosed with subclinical ketosis (SCK) in the early stage of lactation. Cows that calved from January 2011 through December 2014 on a dairy farm with alarm level prevalence of SCK in Hokkaido, Japan ( = 175) were used. Blood and body condition scores (BCS) were obtained at regular health examinations in 2 consecutive periods, the first between 14 and 2 d before parturition, and the second between 3 and 14 d after parturition. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318827PMC
January 2019

Comparative molecular analysis of fecal microbiota of bobcats ( and domestic cats .

Can J Vet Res 2019 Jan;83(1):42-49

Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, 1800 Denison Avenue, Manhattan, Kansas 66506, USA (Eshar); Department of Animal Sciences and Industry, Kansas State University, 131 Call Hall, Manhattan, Kansas 66506, USA (Lee); Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Weese).

The goal of this study was to explore and describe fecal microbiota of captive and wild bobcats and compare the results to those of domestic cats . Fecal samples from 27 bobcats (8 wild, 19 zoo-kept) were used for novel bacterial deoxyribonucleic acid (DNA) identification using next-generation sequencing of the V4 region of the bacterial 16S ribosomal ribonucleic acid (rRNA) gene, analyzed by Illumina sequencing, and then compared to data obtained from a colony of 10 domestic cats. In this study, the microbiota of both species was dominated by Firmicutes, followed by Proteobacteria, Actinobacteria, Bacteroidetes, and Verrucomicrobia. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318824PMC
January 2019
1 Read

Changes in antimicrobial susceptibility profiles of over time.

Can J Vet Res 2019 Jan;83(1):34-41

Animal Health Laboratory, Laboratory Services (Cai, McDowall, Parker) and Department of Pathobiology (Kaufman, Caswell), University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1.

is a major cause of pneumonia, arthritis, and mastitis in cattle and can lead to significant economic losses. Antimicrobial resistance is a concern and further limits the already short list of drugs effective against mycoplasmas. The objective of this study was to examine changes in minimum inhibitory concentrations (MICs) of antimicrobials of aminoglycoside, fluoroquinolone, lincosamide, macrolide, pleuromutilin, phenicol, and tetracycline classes for 210 isolates collected from 1978 to 2009. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318825PMC
January 2019
1 Read

Use of standard diagnostic techniques to determine eradication of infection in experimental equine septic arthritis.

Can J Vet Res 2019 Jan;83(1):24-33

Department of Veterinary Pathology (Koziy, Dickinson, Rybicka, Moshynskyy, Simko), Department of Large Animal Clinical Sciences (Yoshimura, Bracamonte), and Prairie Diagnostic Services Inc. (Ngeleka), Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.

Septic arthritis is an important disease in horses, necessitating aggressive and prolonged therapy. In order to guide therapy, reliable methods of detecting the eradication of infection are needed. Therefore, the objective of this study was to investigate detection of eradication of infection in an experimental model of equine septic arthritis using standard diagnostic techniques. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318826PMC
January 2019

The effect of transfixation pins on the biomechanical properties of angled acrylic connecting bars.

Can J Vet Res 2019 Jan;83(1):17-23

Faculty of Veterinary Medicine, Ringgold Standard Institution - Clinical Sciences, University of Montreal, Saint Hyacinthe, Quebec (Montasell, Herndon, Szwec); Faculty of Veterinary Medicine, University of Montreal, Saint Hyacinthe, Quebec (Beauchamp).

With acrylic external-fixation frames for fracture repair the acrylic columns can be contoured to allow greater versatility in the placement of transfixation pins, thus minimizing damage to the surrounding soft tissue and making mandibular and transarticular fixation easier. However, contouring affects the stiffness and ultimate strength of the construct under axial compression. In this study, polymethylmethacrylate columns 21 mm in diameter angled at 0°, 30°, 45°, 60°, or 90° with clamps were constructed. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318828PMC
January 2019

Cochleosaccular (Scheibe) dysplasia in dogs: A temporal bone study.

Can J Vet Res 2019 Jan;83(1):11-16

Department of Internal Medicine (Keskin, Albasan) and Department of Surgery (Sancak), Faculty of Veterinary Medicine, University of Ankara, Ankara, Turkey; Department of Otolaryngology, Head and Neck Surgery, School of Medicine, University of Minnesota, Lions Research Building, Room 210, Mayo Mail Code 2873, 2001 6th Street SE, Minneapolis, Minnesota 55455, USA (Keskin, Albasan, Sancak, Cureoglu); Paparella Ear Head & Neck Institute, Minneapolis, Minnesota, USA (Paparella); Department of Otolaryngology, Massachusetts Eye and Ear Infirmary (Mini-fellowship), Harvard University, Boston, Massachusetts, USA (Keskin).

The objective of this study was to evaluate any otopathologic changes in temporal bone specimens from dogs with deafness related to cochleosaccular (Scheibe) dysplasia (CSD). We used the canine temporal bone collections of the Otopathology Laboratory at the University of Minnesota and of the Massachusetts Eye and Ear Infirmary at Harvard University in Boston. Our morphometric analysis included measuring the areas of the stria vascularis and the spiral ligament and counting the number of spiral ganglion cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318820PMC
January 2019
10 Reads

Evaluation of effects of radiation therapy combined with either pamidronate or zoledronate on canine osteosarcoma cells.

Can J Vet Res 2019 Jan;83(1):3-10

Department of Clinical Studies (Hoddinott, Oblak, Mutsaers) and Department of Pathobiology (Wood), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610-0126, USA (Boston).

Canine osteosarcoma is a devastating disease with an overall poor prognosis. Radiation therapy and bisphosphonates are currently used in combination for palliative treatment, despite a paucity of literature that investigates their combined use. The objectives of this study were to assess the effects of radiation therapy and bisphosphonates on canine osteosarcoma cells when used in combination. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318829PMC
January 2019

fecal shedding in pigs from birth to market and its association with the presence of in palatine tonsils and submandibular lymph nodes at slaughter.

Can J Vet Res 2018 Oct;82(4):249-255

Department of Pathobiology (Ainslie-Garcia, Farzan, Newman, Lillie) and Department of Population Medicine (Farzan, Newman, Friendship), University of Guelph, Guelph, Ontario N1G 2W1.

is an important cause of foodborne illnesses in humans. Food-producing animals, including swine, are a major source of in food products. This study investigated on farm fecal shedding in pigs at different production stages - from weaning to marketing - and its association with the presence of in tissues at slaughter. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168018PMC
October 2018
14 Reads

The effect of graded concentrations of dietary tryptophan on canine behavior in response to the approach of a familiar or unfamiliar individual.

Can J Vet Res 2018 Oct;82(4):294-305

University of Guelph, Department of Animal Biosciences, Guelph, Ontario N1G 2W1 (Templeman, Cant, Osborne, Shoveller); The Iams Company, Procter and Gamble - Pet Care Division, Mason, Ohio, 45040 USA (Davenport, Shoveller).

Tryptophan (Trp) is an indispensable dietary amino acid that supports the modulation of mood and behavior in mammalian species through its role in the serotonergic pathway. This study assessed the behavior patterns of 36 healthy, female adult mixed-breed hound dogs fed a control diet (tryptophan = 0.18% as-fed) or 1 of several experimental diets with graded concentrations of tryptophan (0. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168022PMC
October 2018
7 Reads

A method to detect carrying the colistin-resistance genes and using a single real-time polymerase chain reaction and its application to chicken cecal and porcine fecal samples.

Can J Vet Res 2018 Oct;82(4):312-315

Department of Pathobiology (Chalmers, Davis, Mulvey, Reid-Smith, Boerlin) and Department of Population Medicine, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1 (Poljak, Friendship, Deckert, Reid-Smith); National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba (Mulvey); Centre for Food-borne, Environmental Zoonotic Infectious Diseases, Public Health Agency of Canada, Guelph, Ontario (Deckert, Reid-Smith).

Colistin is one of the last-resort antibiotics for the treatment of multidrug-resistant infections in humans, but transmissible colistin-resistance genes have emerged in bacteria from animals. The rapid and sensitive detection among animals of colonization with bacteria carrying these genes is critical in helping to control further spread. Here we describe a method for broth enrichment of colistin-resistant from animal fecal and cecal samples followed by real-time polymerase chain reaction (PCR) for the simultaneous detection of two of the main colistin-resistance genes, and The PCR uses a single set of nondegenerative primers, and variants can be differentiated by melt-curve analysis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168024PMC
October 2018
3 Reads

Competitive Luminex immunoassays for detection of antibodies to foot-and-mouth disease and vesicular stomatitis viruses in multiple susceptible hosts.

Can J Vet Res 2018 Oct;82(4):316-321

National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba R3E 3M4.

Foot-and-mouth disease (FMD) and vesicular stomatitis (VS) cause such similar clinical signs and lesions that laboratory tests are required to distinguish between infections caused by each virus. Using mouse anti-foot-and-mouth disease virus (FMDV) 3B monoclonal or polyclonal anti-vesicular stomatitis virus-New Jersey (VSV-NJ) antibodies and recombinant FMDV 3ABC or VSV-NJ glycoprotein (G) antigens coated to MagPlex beads, competitive Luminex immunoassays (cLIAs) were developed for FMDV and VSV-NJ, respectively. The cLIAs successfully detected antibodies to FMDV 3ABC and VSV-NJ G in sera from infected animals. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168021PMC
October 2018
12 Reads

Comparison of saphenous and cephalic blood lactate concentrations in dogs with gastric dilatation and volvulus: 45 cases.

Can J Vet Res 2018 Oct;82(4):271-277

Koret School of Veterinary Medicine Department of Emergency and Critical Care, Hebrew University of Jerusalem, Jerusalem, Israel 76100 (Oron, Klainbart, Bruchim, Kelmer, Saar); Faculty of Veterinary Medicine, Veterinary Clinical and Diagnostic Sciences, University of Calgary, Calgary, Alberta T2N 1N4 (McMurray, Boysen).

Gastric dilatation and volvulus (GDV) is a life-threatening condition, suspected to result in differential tissue perfusion in different regions of the body. Lactate is a biomarker of hypoperfusion that correlates with treatment outcomes in dogs with GDV. This prospective observational study aimed to compare lactate between saphenous and cephalic venous samples in 45 client-owned dogs with GDV, to evaluate their association with treatment outcomes (survival, surgical complications, and duration of hospitalization) and to assess the correlation between lactate and other biochemical parameters. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168020PMC
October 2018
18 Reads
1.022 Impact Factor

Molecular epidemiology, antimicrobial susceptibility, and pulsed-field gel electrophoresis genotyping of isolates from mink.

Can J Vet Res 2018 Oct;82(4):256-263

College of Veterinary Medicine, National Risk Assessment Laboratory for Antimicrobial Resistance of Microorganisms in Animals, South China Agricultural University, 483 WuShan Road, Tianhe District, Guangzhou 510642, China (Zhao, Li, Fang, Huang); Qingdao Yebio Biological Engineering Company Ltd., Qingdao, China (Guo).

is an important animal pathogen and contributes to hemorrhagic pneumonia in mink. Between April 2011 and December 2016, samples of lung, liver, and spleen were collected from mink with this disease on 11 mink farms in 5 Chinese provinces. From these samples, we obtained 98 isolates of that belonged to 5 serotypes: G ( = 58), I ( = 15), C ( = 8), M ( = 5), and B ( = 2); 10 isolates were not typeable (10/98). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168023PMC
October 2018
15 Reads
1.022 Impact Factor

Use of platelet-rich plasma for the treatment of prostatic cysts in dogs.

Can J Vet Res 2018 Oct;82(4):264-270

Department of Veterinary Medical Science, University of Parma, Via del Taglio 10, 43100 Parma, Italy.

Abnormalities of the canine prostate gland, in particular, benign hyperplasia and cysts, are frequent in dogs over 5 years of age. Treatment strategies for dogs with prostatic cysts include ultrasound-guided percutaneous drainage with alcohol sclerotherapy, reduction surgery, and omentalization. The aim of this study was to evaluate the use of platelet-rich plasma (PRP) for the treatment of prostatic cysts in dogs. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168016PMC
October 2018
11 Reads

Hematological and serum biochemical profile values in pregnant and non-pregnant mares.

Can J Vet Res 2018 Oct;82(4):287-293

College of Veterinary Medicine, Western University of Health Sciences, Pomona, California (Faramarzi); Antech Diagnostics, Irvine, California (Rich); Pacific Northwest University of Health Sciences, Yakima, Washington (Wu).

Pregnancy in many mammals, including mares, is associated with physiological changes that are reflected in hematological and biochemical profiles. Understanding those physiological changes and differentiating them from pathological changes is imperative for providing care and medical therapy in pregnant mares. Our objective was to compare normal hematological and biochemical profiles in healthy non-pregnant as well as healthy pregnant mares during the 1st and 2nd trimesters of pregnancy. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168017PMC
October 2018
55 Reads

Effects of immunosuppressive prednisolone therapy on pancreatic tissue and concentration of canine pancreatic lipase immunoreactivity in healthy dogs.

Can J Vet Res 2018 Oct;82(4):278-286

Laboratory of Veterinary Internal Medicine (Ohta, Kojima, Yokoyama, Sasaki, Takiguchi), Diagnostic Pathology (Kagawa), and Veterinary Teaching Hospital (Hanazono, Ishizuka, Morishita, Nakamura, Takagi), Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Sapporo, Hokkaido 060-0818, Japan.

The objective of this study was to examine the effects of immunosuppressive prednisolone therapy on pancreatic tissue and the concentration of serum canine pancreatic lipase immunoreactivity (cPLI) in healthy dogs. Six healthy beagle dogs were subcutaneously administered an immunosuppressive dose of prednisolone [4 mg/kg body weight (BW)] once daily for either 2 or 3 weeks. Serum cPLI concentration was measured before and after treatment. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168014PMC
October 2018
2 Reads

Correlation between transepidermal water loss (TEWL) and severity of clinical symptoms in cats with atopic dermatitis.

Can J Vet Res 2018 Oct;82(4):306-311

Subdepartment of Clinical Diagnostics and Veterinary Dermatology (Szczepanik, Wilkołek, Adamek, Kalisz, Sitkowski, Taszkun) and Subdepartment of Internal Diseases of Farm Animals and Horses (Gołyński), Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.

The aim of this study was to demonstrate whether there is a correlation between transepidermal water loss (TEWL) and the severity of skin lesions in cats with feline atopic dermatitis (AD). The severity of symptoms was determined by using 2 scoring systems for clinically assessing the skin lesions in cats: Scoring Feline Allergic Dermatitis (SCORFAD) and Feline Extent and Severity Index (FeDESI). Such a correlation between TEWL and systems for clinically evaluating symptoms has already been shown in humans and dogs. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168015PMC
October 2018
10 Reads

Oscillometric and Doppler arterial blood pressure measurement in conscious goats.

Can J Vet Res 2018 Oct;82(4):244-248

Department of Small Animal Diseases with Clinic (Szaluś-Jordanow), Laboratory of Veterinary Epidemiology and Economics (Czopowicz, Mickiewicz, Moroz, Kaba), and Department of Pathology and Veterinary Diagnostics (Garncarz, Szpinda), Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland; Round-the-clock Veterinary Clinic LEGWET, Legionowo, Poland (Świerk); Institute of Genetics and Animal Breeding, Polish Academy of Science, Magdalenka, Poland (Bagnicka).

The objective of this study was to characterize arterial blood pressure (BP) measurements obtained by using 2 indirect methods, oscillometry and Doppler ultrasonic sphygmomanometry, in conscious goats. Agreement between systolic BP yielded by these 2 methods was then assessed. Sixty female dairy goats aged from 1. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168019PMC
October 2018
9 Reads

Effect of piglet separation from dam at birth on colostrum uptake.

Can J Vet Res 2018 Jul;82(3):239-242

Animal Science Department, California Polytechnic State University, San Luis Obispo, California, USA (Manjarín, Montano, Bennet); School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia 5371, Australia (Kirkwood, Petrovski); Australian Centre for Antimicrobial Resistance Ecology, University of Adelaide, Adelaide, South Australia, Australia (Petrovski); Davies Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia, 5371, Australia (Petrovski).

The objective of this study was to determine whether birth order influences piglet survival because of reduced uptake of maternal antibodies by the piglets born later in large litters. Forty-five litters were serially allocated to one of 2 study groups. The crèche group consisted of 18 litters for which the 205 piglets were removed to a warm box to prevent suckling until 4 h after delivery of the first pig, and the control group of 27 litters for which 306 piglets were allowed to suckle from birth. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040012PMC
July 2018
23 Reads

gene variants as potential phenotype and performance biomarkers in Brazilian sport horses training for eventing in a tropical climate.

Can J Vet Res 2018 Jul;82(3):236-238

Programa de Pós-Graduação em Medicina Veterinária (Clínica e Reprodução Animal), Universidade Federal Fluminense, Rua Vital Brasil Filho, 64-Vital Brasil, Niterói, Rio de Janeiro, Brazil 24230-340 (Padilha, Ferreira); Departamento de Genética e Biologia Molecular, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Brazil (El-Jaick); Laboratório de Pesquisa em Farmacogenética, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil (de Castro); Plataforma Genômica-Sequenciamento de DNA, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil (Moreira); Laboratório de Avaliação do Desempenho de Equinos, Escola de Equitação do Exército, Rio de Janeiro, Brazil (Padilha, de Almeida, Ferreira); Departamento de Medicina e Cirurgia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil (de Almeida); Laboratório de Biologia Molecular do Banco de Tecidos e Material Genético de Animais Silvestres e Domésticos, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil (Padilha, Ferreira); Departamento de Patologia e Clínica Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil (Ferreira).

The aim of this study was to look for mutations in the equine gene and to identify sequence variants that might be associated with the phenotype and performance of Brazilian sport horses training for events in a tropical climate. Among 17 such horses direct DNA sequencing and mutation analysis of the exon 15 and the intron-exon boundaries of revealed 2 new sequence variants in the intron 14-15, designated c.1681-86G > A and c. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040019PMC
July 2018
6 Reads

Isolation, culture, and characterization of chicken lung-derived mesenchymal stem cells.

Can J Vet Res 2018 Jul;82(3):225-235

Research Center for Sports Scientific Experiment, Harbin Institute of Physical Education, Harbin, Heilongjiang Province, 150008, P.R. China (Wang X, C, Wang JJ, Ji, Zhao); Department of Animal Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China (Wang X, Guan).

Using lung tissues separated from 12-day-old chicken embryos, we attempted to obtain a novel population of stem cells, namely, chicken lung-derived mesenchymal stem cells (LMSCs), which exhibit spindle-like morphology. The results of colony-forming assay and population doubling assay demonstrated that LMSCs had enormous colony-forming, self-renewal, and proliferative potential. When appropriately induced, LMSCs could differentiate into osteoblasts, adypocytes, chondrocytes, and neurons; in other words, LMSCs had cross-embryonic layer differentiation potential under corresponding induction conditions. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040015PMC
July 2018
24 Reads

The biological characteristics of sheep umbilical cord mesenchymal stem cells.

Can J Vet Res 2018 Jul;82(3):216-224

Institute of Kinesiology, Harbin Sport University, Harbin, P.R. China (Chen, Zhao C, Zhao Y, Li, Liu, Zhu), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Chen, Guan).

Although mesenchymal stem cells (MSCs) are now regarded as a promising cell resource for tissue repair and regeneration, the optimal source of MSCs has not yet been determined. The objective of this study was to provide a theoretical basis for the clinical application of umbilical cord mesenchymal stem cells (UCMSCs) in the future. Umbilical cord is an easily obtainable tissue resource, which is one reason that it has become a candidate resource for mesenchymal stem cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040013PMC
July 2018
8 Reads
1.020 Impact Factor

Intraocular pressure measurements in cattle, sheep, and goats with 2 different types of tonometers.

Can J Vet Res 2018 Jul;82(3):208-215

Freie Universität Berlin, Department of Veterinary Medicine, Small Animal Clinic, Oertzenweg 19b, Berlin 14163, Germany.

The aim of this study was to investigate normal intraocular pressure (IOP) values of cattle, sheep, and goats with a rebound tonometer [TonoVet (TV)] and an applanation tonometer [Tono-Pen AVIA (TPA)] and to determine correction functions for the 2 devices. A total of 60 healthy cattle, sheep, and goats (20 of each) underwent slit-lamp biomicroscopy. Intraocular pressure (IOP) readings were taken from both eyes with the 2 different tonometers and statistically analyzed. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040018PMC

Effects of a single intravenous bolus injection of alfaxalone on canine splenic volume as determined by computed tomography.

Can J Vet Res 2018 Jul;82(3):203-207

Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, Florida 32610, USA (Hasiuk, Berry, Ellison); Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, Florida 32610, USA (Garcia-Pereira).

The purpose of this study was to evaluate the effects of a single intravenous dose of alfaxalone on canine splenic volume. In 6 adult beagle dogs the splenic volume [mean ± standard error (SE)] was determined by computed tomography to be 0.17 ± 0. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040021PMC
July 2018
1 Read

Cardiac weights and weight ratios as indicators of cardiac lesions in pigs: A study of pig hearts from an Ontario abattoir.

Can J Vet Res 2018 Jul;82(3):198-202

Department of Population Medicine, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1 (Zurbrigg, Friendship, O'Sullivan); Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, Iowa 50011, USA (Rothschild).

Clinically healthy pigs used in research are assumed to have normal cardiac structure and function. Subclinical cardiac abnormalities may adversely affect the responses being measured in these experiments. The gross and histologic lesions observed in hearts collected from a Canadian abattoir between 2012 and 2015 indicated an unexpectedly high prevalence of cardiac abnormalities: 75% (297/396) of the hearts examined had such lesions. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040014PMC
July 2018
11 Reads

Impact of a trap-neuter-return event on the size of free-roaming cat colonies around barns and stables in Quebec: A randomized controlled trial.

Can J Vet Res 2018 Jul;82(3):192-197

Department of Clinical Sciences (Bissonnette, Lussier) and Department of Pathology and Microbiology (Doizé, Arsenault), Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec J2S 7C6.

The objective of this study was to evaluate the impact of a trap-neuter-return (TNR) event on the size of free-roaming rural cat colonies in Quebec. This prospective randomized, controlled study included 18 cat colonies around barns and stables that were randomly assigned to either a TNR group (10 colonies of 7 to 27 cats; 14.3 cats on average) or a control group (8 colonies of 7 to 26 cats; 14. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040017PMC

Does antimicrobial therapy improve outcomes in horses with severe equine asthma and a positive tracheal wash bacterial culture?

Can J Vet Res 2018 Jul;82(3):184-191

Department of Large Animal Clinical Sciences (Husulak, Manning, Epp, Montgomery) and Department of Veterinary Pathology (Meachem, Burgess), Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.

The objective of this study was to observe the outcomes of adding an antimicrobial treatment to a conventional treatment regime in horses with severe equine asthma in a clinical setting. Eleven client-owned horses with a history consistent with severe equine asthma, increased respiratory effort and nostril flaring, ≥ 20% neutrophils on bronchoalveolar lavage (BAL), and a positive tracheal wash (TW) bacterial culture were treated with environmental management, corticosteroids, and bronchodilators. Six horses were also treated with an antimicrobial (principal group), while the other 5 were administered saline as a placebo (control group). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040016PMC
July 2018
12 Reads

Pharmacokinetics of cannabidiol administered by 3 delivery methods at 2 different dosages to healthy dogs.

Can J Vet Res 2018 Jul;82(3):178-183

Department of Clinical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, Colorado 80523, USA.

The purpose of this study was to determine the pharmacokinetics of cannabidiol (CBD) in healthy dogs. Thirty, healthy research dogs were assigned to receive 1 of 3 formulations (oral microencapsulated oil beads, oral CBD-infused oil, or CBD-infused transdermal cream), at a dose of 75 mg or 150 mg q12h for 6 wk. Serial cannabidiol plasma concentrations were measured over the first 12 h and repeated at 2, 4, and 6 wk. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6038832PMC
July 2018
64 Reads

Changes in antimicrobial resistance levels among and in Ontario broiler chickens between 2003 and 2015.

Can J Vet Res 2018 Jul;82(3):163-177

Public Health Agency of Canada, Guelph (Agunos, Avery, Deckert, Janecko, Léger, Parmley, Reid-Smith); Canadian Food Inspection Agency, Ottawa (Arsenault); Ontario Veterinary College, University of Guelph, Guelph, Ontario (McEwen); Public Health Agency of Canada, Saskatoon (Gow).

Poultry has been identified as a reservoir of foodborne enteric pathogens and antimicrobial resistant bacteria. The objective of this study was to describe and compare antimicrobial resistant isolates from an Ontario broiler chicken farm-level baseline project (2003 to 2004) to the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) Ontario abattoir and retail surveillance data from 2003, and to the most recent (2015) CIPARS Ontario chicken surveillance data in order to assess the impact of an industry-wide policy change in antimicrobial use. Ceftiofur resistance (TIO-R) prevalence in decreased by 7% on farm between 2003 and 2004 and 2015. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6038833PMC
July 2018
2 Reads

Peak vertical force in a stabilized canine cranial cruciate deficient stifle model: A one-year follow-up.

Can J Vet Res 2018 Apr;82(2):159-161

Quebec Animal Pharmacology Research Group (Groupe de recherche en pharmacologie animale du Québec; GREPAQ), Université de Montréal, Saint-Hyacinthe, Quebec J2S 2M2 (Lussier, Gagnon, Moreau, Troncy); Osteoarthritis Research Unit, Centre hospitalier de l'Université de Montréal, Hôpital Notre-Dame, Montreal, Quebec H2L 4M1 (Lussier, Moreau, Pelletier, Troncy).

This study aimed to describe the peak vertical force (PVF) over a 1-year period in a stabilized canine cranial cruciate deficient stifle model. Our hypothesis was that PVF would be restored to Baseline (intact) at the end of the follow-up. Fifteen (> 20 kg) mixed-breed dogs were included in this study. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914080PMC
April 2018
2 Reads

Effect of a commercially available fish-based dog food enriched with nutraceuticals on hip and elbow dysplasia in growing Labrador retrievers.

Can J Vet Res 2018 Apr;82(2):154-158

Department of Veterinary Medical Science, University of Parma, Via del Taglio 10, 43100 Parma, Italy (Manfredi, Di Ianni, Gnudi, Miduri, Fabbi, Daga, Parmigiani, Volta); Centro Veterinario Specialistico, Via Sandro Giovannini, 51/53, 00137 Rome, Italy (Di Girolamo); Forza10 USA Corporation, Research and Development Department, 6450 Kingspointe Parkway, Orlando, Florida 32819, USA (Canello); Sanypet, Research and Development Department, Via Austria 3, 35023 Bagnoli di Sopra, Padua, Italy (Guidetti, Centenaro).

The aim of this study was to evaluate the prevalence of hip and elbow dysplasia in a group of growing Labrador retrievers fed a fish-based diet enriched with nutraceuticals with chondroprotective properties. The puppies ranged from 3 to 12 mo of age and were divided into 2 groups, each fed a different diet. The control diet consisted of a high quality, chicken-based dog food, while the test diet was a fish-based dog food, enriched with nutraceuticals. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914087PMC
April 2018
10 Reads

Evaluation of the efficacy of a novel porcine circovirus type 2 synthetic peptide vaccine.

Can J Vet Res 2018 Apr;82(2):146-153

Seoul National University, College of Veterinary Medicine, Department of Veterinary Pathology, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea.

A novel porcine circovirus type 2 (PCV2) peptide vaccine comprised of a consensus capsid (Cap) protein domain encoded by open reading frame 2 was developed to control PCV2 infection. The efficacy of the vaccine was evaluated against a commercial baculovirus-expressed recombinant PCV2 subunit vaccine based on the Cap protein. The amino acid sequence of this Cap protein was designed based on the alignment of amino acid sequences from different isolates from Europe, North America, and Asia. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914084PMC
April 2018
1 Read

Time course of shedding and antibody response in naturally infected pigs during grower-finisher stage.

Can J Vet Res 2018 Apr;82(2):139-145

Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.

A longitudinal trial was conducted to determine the course of shedding and antibody response in naturally infected grower-finisher pigs. Ten-week-old pigs ( = 45) were transferred from a farm with history of salmonellosis and housed at a research facility. Weekly fecal samples (weeks 1 to 11) as well as tissue samples at slaughter were cultured for Serum samples were tested for presence of antibody by enzyme-linked immunosorbent assay (ELISA). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914076PMC
April 2018
2 Reads

H9N2 avian influenza virus retained low pathogenicity after serial passage in chickens.

Can J Vet Res 2018 Apr;82(2):131-138

Ottawa Laboratory (Fallowfield), Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, Ontario (Jegede, Fu, Lin, Guan); National Centre for Foreign Animal Disease (Winnipeg Laboratory - Arlington), 1015 Arlington Street, Winnipeg, Manitoba (Berhane); Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ontario (Lin, Kumar).

The H9N2 strains of avian influenza viruses (AIVs) circulate worldwide in poultry and cause sporadic infection in humans. To better understand the evolution of these viruses while circulating in poultry, an H9N2 chicken isolate was passaged 19 times in chickens aerosol inoculation. Whole-genome sequencing showed that the viruses from the initial stock and those after the 8th and 19th passages (P0, P8, and P19) all had the same monobasic cleavage site in the hemagglutinin (HA), typical for viruses of low pathogenicity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914077PMC
April 2018
25 Reads
1.022 Impact Factor

Efficacy and pharmacokinetics of bupivacaine with epinephrine or dexmedetomidine after intraperitoneal administration in cats undergoing ovariohysterectomy.

Can J Vet Res 2018 Apr;82(2):124-130

Department of Clinical Sciences (Benito, Steagall), Biomedical Sciences (Monteiro, Beaudry), and Animal Pharmacology Research Group of Quebec (GREPAQ; Groupe de recherche en pharmacologie animale du Québec) (Monteiro, Beaudry, Steagall), Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, Quebec J2S 2M2.

The aim of this study was to determine the efficacy and pharmacokinetics of bupivacaine in combination with epinephrine or dexmedetomidine after intraperitoneal administration in cats undergoing ovariohysterectomy. Sixteen healthy adult cats (3.3 ± 0. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914079PMC
April 2018
15 Reads

26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumors.

Can J Vet Res 2018 Apr;82(2):115-123

Institute of Veterinary Medicine, Georg August University of Goettingen, Burckhardtweg 2, (Gerke, Neumann); Pathology Unit, German Primate Center, Leibniz-Institute for Primate Research, Kellnerweg 4, 37077 Goettingen, Germany (Kaup).

Studies in humans have shown that the ubiquitin-proteasome pathway and the insulin-like growth factor axis are involved in carcinogenesis, thus, components of these systems might be useful as prognostic markers and constitute potential therapeutic targets. In veterinary medicine, only a few studies exist on this topic. Here, serum concentrations of 26S proteasome (26SP) and insulin-like growth factor-1 (IGF-1) were measured by canine enzyme-linked immunosorbent assay (ELISA) in 43 dogs suffering from malignant tumors and 21 clinically normal dogs (control group). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914081PMC

Venous blood gases, plasma biochemistry, and hematology of wild-caught common chameleons .

Can J Vet Res 2018 Apr;82(2):106-114

Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, 1800 Denison Avenue, Manhattan, Kansas 66506, USA (Eshar); Department of Pathobiology (Ammersbach) and Health Sciences Center (Beaufrère), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1; National Natural History Collections, The Hebrew University of Jerusalem, E. Safra Campus, Jerusalem 9190401, Israel (Shacham); Department of Evolutionary and Environmental Biology, University of Haifa, 199 Aba Khoushy Avenue, Mount Carmel, Haifa 3498838, Israel (Katzir).

The purpose of this study was to determine a wide range of selected hematologic, venous blood gases, and plasma biochemistry analytes in common chameleons . Blood samples were collected from the ventral tail vein of 41 common chameleons to determine reference intervals for 30 different blood analytes. The calcium-to-phosphorus ratio, packed cell volume (PCV), refractometric total solids (TS), blood cell counts, and differentials were also determined. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914082PMC
April 2018
2 Reads

Roles of the and genes of serovar Typhimurium in protective efficacy and immune responses to vaccination in mice.

Can J Vet Res 2018 Apr;82(2):102-105

The Key Laboratory of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, China (Chen, Liao, Zhang, Cheng); Luoyang Key Lab of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, China (Chen, Liao, Zhang, Cheng); College of Veterinary Medicine, Northwest A&F University, Yangling, China (Chen).

serovar Typhimurium has a wide host range and is capable of causing infections ranging from severe gastroenteritis to systemic infection in humans. To determine if attenuated Typhimurium strains can serve as safe and effective oral vaccines to prevent typhoid fever, the biologic characteristics of and deletion mutants were evaluated. Previous studies had found that the and genes are related to pathogenicity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914075PMC
April 2018
4 Reads

Comparison between cerebrospinal fluid and serum lactate concentrations in neurologic dogs with and without structural intracranial disease.

Can J Vet Res 2018 Apr;82(2):97-101

School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6009, USA.

The objectives of this study were to investigate the relationship between cerebrospinal fluid lactate and serum concentrations in dogs with clinical signs of central nervous system disease and to establish if cerebrospinal fluid lactate (CSF) concentrations are higher in dogs with structural intracranial disease (Group Pos-MRI) compared to dogs that have clinical signs of intracranial disease but no structural brain disease (Group Neg-MRI) based on magnetic resonance imaging (MRI) findings. Using a prospective study canine blood and cerebrospinal fluid were collected in 24 dogs with neurological signs after undergoing brain MRI. Dogs were divided in 2 groups. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914086PMC

Using a computer simulation model to examine the impact of biosecurity measures during a facility-level outbreak of equine influenza.

Can J Vet Res 2018 Apr;82(2):89-96

Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.

On-farm biosecurity measures are an important part of a control plan to minimize the introduction and spread of infectious diseases, such as equine influenza, in an equine facility. It can be challenging, however, to evaluate the efficacy of biosecurity measures under field conditions. We used an agent-based computer simulation model to describe the impact of: i) preventive vaccination; ii) reduced horse-to-horse contact; and iii) a combination of vaccination and reduced contact during an outbreak of equine influenza in a simulated horse facility. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914078PMC

study of matrix metalloproteinases 1, 2, 9, 13 and serum amyloid A mRNAs expression in equine fibroblast-like synoviocytes treated with doxycycline.

Can J Vet Res 2018 Apr;82(2):82-88

Section of Surgery and Radiology, Department of Clinical Sciences (Ghasemi, Sardari); Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Razavi Khorasan, Iran (Mirshokraei); Department of Basic Sciences, Physiology Division, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Chaharmahal and Bakhtiari, Iran (Hassanpour).

Application of synthetic matrix metalloproteinases (MMPs) inhibitors, such as doxycycline is one of the possible therapeutic options for osteoarthritis. However, little is known about the protective mechanism of doxycycline in equine models on MMPs inhibitors as well as on serum amyloid A (SAA) gene expression. This study investigated the effects of doxycycline on mRNA expression of MMP-1, MMP-2, MMP-9, MMP-13, and SAA of equine fibroblast-like synoviocytes (FLSs). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914083PMC
April 2018
6 Reads

Effect of transoral tracheal wash on respiratory mechanics in dogs with respiratory disease.

Can J Vet Res 2018 Jan;82(1):75-79

Section of Critical Care, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts 01536, USA.

The purpose of this study was to determine the impact of a transoral tracheal wash (TOTW) on respiratory mechanics in dogs and to describe the use of a critical care ventilator (CCV) to determine respiratory mechanics. Fourteen client-owned dogs with respiratory diseases were enrolled. Respiratory mechanics, including static compliance (C) and static resistance (R), were determined before and after TOTW. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764042PMC
January 2018
1 Read

Development and application of a droplet digital polymerase chain reaction (ddPCR) for detection and investigation of African swine fever virus.

Can J Vet Res 2018 Jan;82(1):70-74

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China (Xulong Wu, Yin Wang, Zexiao Yang, Xueping Yao); Sichuan Animal Science Academy, Chengdu, 610066, China (Lu Xiao); Key Laboratory of Animal Genetics and Breeding of Sichuan Province, Chengdu, 611130, China (Lu Xiao); Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China (Yin Wang, Zexiao Yang, Xueping Yao); Inspection and Quarantine Technical Center, Sichuan entry-exit inspection and Quarantine Bureau, Chengdu 610041, China (Hua Lin, Shijie Chen, Miao Yang, Wei An); College of Life Science, Sichuan Agricultural University, Yaan 625014, China (Zizhong Tang).

The aim of this study was to develop a droplet digital polymerase chain reaction (ddPCR) method to detect African swine fever virus (ASFV). The methods of ASFV real-time PCR and ddPCR were established and optimal reaction conditions were confirmed. Each method was evaluated for linearity, limit of detection, and specificity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764039PMC
January 2018
10 Reads

Livestock-associated methicillin-resistant and in wild Norway rats from Ontario swine farms.

Can J Vet Res 2018 Jan;82(1):66-69

Department of Pathobiology (Rothenburger, Rousseau, Weese, Jardine) and Canadian Wildlife Health Cooperative Ontario-Nunavut Region (Rothenburger, Jardine), Ontario Veterinary College, University of Guelph, Guelph, Ontario.

Methicillin-resistant (MRSA) and are important human pathogens that are also carried by animals. The role of wild mammals on farms in their maintenance and transmission, however, is poorly understood. To determine if Norway rats are potential carriers of these bacteria on Canadian farms, we tested 21 rats from swine farms in Ontario. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764037PMC
January 2018
3 Reads

Histological structure and age-related changes in the luminal diameter of the excurrent duct system of guinea cocks and associated changes in testosterone concentrations.

Can J Vet Res 2018 Jan;82(1):60-65

Department of Veterinary Science, Faculty of Agriculture, University for Development Studies, P.O. Box TL 1882, Nyankpala Campus, Tamale, Ghana (Abdul-Rahman); Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow G61 1QH, Scotland, UK (Jeffcoate).

As little information is available on the reproductive system of guinea fowl a study was conducted on 49 male guinea fowl to document the histological structure and developmental changes in the luminal diameter of the ducts within the excurrent duct system and associated changes in concentrations of testosterone. Age-related changes were analyzed using the Kruskal-Wallis test and medians separated by the Mann-Whitney U-test. Tubuli recti were clearly visible in the guinea fowl and the rete testes were both intracapsular and extracapsular. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764047PMC
January 2018
3 Reads

Comparison of the oral and rectal mucosal and colonic serosal microcirculations of healthy, anesthetized horses.

Can J Vet Res 2018 Jan;82(1):55-59

Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA (Kieffer, Williams, Giguère, Epstein); MedVet Medical & Cancer Centers for Pets, Chicago, Illinois, USA (Shepard).

The objectives of the study were to: i) determine baseline microvascular perfusion indices (MPI) and assess their repeatability in healthy horses under general anesthesia, and ii) compare the MPIs of 3 microvascular beds (oral mucosa, colonic serosa, and rectal mucosa). Healthy adult horses were anesthetized and sidestream dark field microscopy was used to collect video loops of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions without cardiovascular support drugs; videos were later analyzed to produce MPIs. Baseline MPI values were determined for each site, which included the total vessel density (TVD), perfused vessel density (PVD), portion perfused vessels (PPV), and microcirculatory flow index (MFI). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764044PMC
January 2018
3 Reads

Biological characteristics and conjugated antigens of ClfA A-FnBPA and CP5 in .

Can J Vet Res 2018 Jan;82(1):48-54

College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, China (Li, Song, Zhang, Chen); College of Life Science, Shihezi University, 832000, Shihezi, Xinjiang, China (Huang).

To obtain immunogenic conjugate antigens, adipic acid dihydrazide (ADH), as a bridge, and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride (EDAC), as a coupling agent, were used to conjugate the purified fusion protein, clumping factor A-fibronectin binding protein ClfA A-FnBPA, and type 5 capsular polysaccharide (CP5). The conjugates were mixed with an adjuvant, and mice were immunized 3 times and challenged with 1 week later. Antibody titers were determined by indirect enzyme-linked immunosorbent assay (ELISA). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764038PMC
January 2018