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Slow and stepped re-warming after acute low temperature exposure do not improve survival of Drosophila melanogaster larvae.

Can Entomol 2008 ;140(3):306-311

Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada.

We tested that hypothesis that slow re-warming rates would improve the ability of Drosophila melanogaster Meigen larvae to survive acute low temperature exposure. Four larval stages (1(st), 2(nd), 3(rd) instars and wandering stage 3(rd) instars) of four wild-type strains were exposed to -7 degrees C for periods of time expected to result in 90 % mortality. Larvae were then either directly transferred to their rearing temperature (21 degrees C), or returned to this temperature in a stepwise fashion (pausing at 0 and 15 degrees C) or by slow warming at 1 or 0. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528286PMC
January 2008
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