9,762 results match your criteria Burkholderia


Clinico-microbiological description and evaluation of rapid lateral flow immunoassay and PCR for detection of Burkholderia pseudomallei from patients hospitalized with sepsis and pneumonia: A twenty-one months study from Odisha, India.

Acta Trop 2021 Jun 9:105994. Epub 2021 Jun 9.

Department of Community and Family Medicine, AIIMS, Bhubaneswar. Electronic address:

Establishing a diagnosis of melioidosis based on clinical grounds is difficult in hospitalized patients with sepsis or community acquired pneumonia (CAP). We aimed to ascertain the prevalence, clinico-epidemiological and laboratory profile of melioidosis in hospitalized patients with sepsis or CAP, and to evaluate the diagnostic utility of rapid lateral flow immunoassay (LFI) and PCR in comparison with culture. In all patients with sepsis or CAP, blood, sputum/throat swab, and urine sample were subjected to culture along with other samples based on clinical presentation. Read More

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Disruption of biofilms and killing of Burkholderia cenocepacia from cystic fibrosis lung using an antioxidant-antibiotic combination therapy.

Int J Antimicrob Agents 2021 Jun 8:106372. Epub 2021 Jun 8.

Department of Infectious Diseases and Immunology, School of Medical Science, The University of Sydney. Electronic address:

Cystic fibrosis (CF) is a disease caused by mutations in the CF transmembrane conductance regulator. The resulting chloride and bicarbonate imbalance produces a thick static lung mucus. This mucus is not easily expelled from the lungs, and so can be colonised by bacteria, leading to biofilm formation. Read More

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One-step, multiplex, dual-function oligonucleotide of loop-mediated isothermal amplification assay for the detection of pathogenic Burkholderia pseudomallei.

Anal Chim Acta 2021 Aug 24;1171:338682. Epub 2021 May 24.

Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia; Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address:

This study highlights the development of a multiplex real-time loop-mediated isothermal amplification assay. The developed assay employed a dual-function oligonucleotide (DFO) which simultaneously monitors the emitted amplification signals and accelerates the amplification process. The DFO was a modification of loop primer (LP); the 5'-end and 3'-end of the LP was tagged with fluorophore and quencher, respectively. Read More

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Antibiotic Cycling Reverts Extensive Drug Resistant .

Antimicrob Agents Chemother 2021 Jun 7:AAC0061121. Epub 2021 Jun 7.

Biological Sciences Department, University of North Carolina at Charlotte, Charlotte, NC.

Antibiotic collateral sensitivity, in which acquired resistance to one drug leads to decreased resistance to a different drug, occurs in . Here we observed that treatment of extensive drug resistant variants from a CF sputum sample with either meropenem or sulfamethoxazole/trimethoprim, depending on past resistance phenotypes, resulted in increased sensitivity to five different classes of antibiotics. We further identified mutations that may be involved in this phenotype, including putative Resistance-Nodulation-Division efflux pump regulators and uncharacterized pumps in Read More

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Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for lectin (BambL).

Chem Sci 2020 Oct 21;11(47):12662-12670. Epub 2020 Oct 21.

Università di Firenze, Department of Chemistry via della Lastruccia, 3, 13, 50019 Sesto F.no FI Italy

Two orthogonal, metal free click reactions, enabled to glycosylate ubiquitin and its mutant A28C forming two protein scaffolds with high affinity for BambL, a lectin from the human pathogen . A new fucoside analogue, with high affinity with BambL, firstly synthetized and co-crystallized with the protein target, provided the insights for sugar determinants grafting onto ubiquitin. Three ubiquitin-based glycosides were thus assembled. Read More

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October 2020

Genetic Diversity and Characterization of Symbiotic Bacteria Isolated from Endemic Phaseolus Cultivars Located in Contrasting Agroecosystems in Venezuela.

Microbes Environ 2021 ;36(2)

Institute of Agriculture, Tokyo University of Agriculture and Technology (TUAT).

Phaseolus vulgaris is a grain cultivated in vast areas of different countries. It is an excellent alternative to the other legumes in the Venezuelan diet and is of great agronomic interest due to its resistance to soil acidity, drought, and high temperatures. Phaseolus establishes symbiosis primarily with Rhizobium and Ensifer species in most countries, and this rhizobia-legume interaction has been studied in Asia, Africa, and the Americas. Read More

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January 2021

Continuous production of monoacylglycerol via glycerolysis of babassu oil by immobilized Burkholderia cepacia lipase in a packed bed reactor.

Bioprocess Biosyst Eng 2021 Jun 5. Epub 2021 Jun 5.

Department of Chemical Engineering, School of Engineering of Lorena, University of São Paulo (USP), 12.602-810, Lorena, SP, Brazil.

This study investigated the glycerolysis of babassu oil by Burkholderia cepacia lipase immobilized on SiO-PVA particles in a continuous packed bed reactor. Experiments were conducted in a solvent-free system at 273.15 K either in an inert atmosphere or in the presence of cocoa butter to prevent lipid oxidation. Read More

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Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning.

Sci Rep 2021 Jun 4;11(1):11883. Epub 2021 Jun 4.

Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University, Tsushima, Okayama, 700-8530, Japan.

We developed a method to improve protein thermostability, "loop-walking method". Three consecutive positions in 12 loops of Burkholderia cepacia lipase were subjected to random mutagenesis to make 12 libraries. Screening allowed us to identify L7 as a hot-spot loop having an impact on thermostability, and the P233G/L234E/V235M mutant was found from 214 variants in the L7 library. Read More

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Contributions of meat waste decomposition to the abundance and diversity of pathogens and antibiotic-resistance genes in the atmosphere.

Sci Total Environ 2021 Aug 16;784:147128. Epub 2021 Apr 16.

Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Guangdong Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Institute of Environmental Health and Pollution control, Guangdong University of Technology, Guangzhou 510006, China; Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Key Laboratory of City Cluster Environmental Safety and Green development, Department of Education, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:

Airborne transmission of antibiotic-resistance genes (ARGs) in landfill and acquisition of antibiotic resistance by pathogenic bacteria are posing potential threat to human and environmental health. However, little is known about contribution of waste decomposition to airborne ARGs and pathogens during landfilling of household waste. Herein, the dynamic changes of microbial communities and ARGs were comparatively investigated in leachate and bioaerosol during the decomposition of chicken, fish, and pork wastes. Read More

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Comparative Genomic Analysis of the Foodborne Pathogen pv. Harboring a Bongkrekic Acid Biosynthesis Gene Cluster.

Front Microbiol 2021 17;12:628538. Epub 2021 May 17.

NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Sciences Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing, China.

The environmental bacterium pv. () has been linked to fatal food poisoning cases in Asia and Africa. Bongkrekic acid (BA), a mitochondrial toxin produced by , is thought to be responsible for these outbreaks. Read More

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Does Cefiderocol Have a Potential Role in Cystic Fibrosis Pulmonary Exacerbation Management?

Microb Drug Resist 2021 Jun 1. Epub 2021 Jun 1.

Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Brooklyn, New York, USA.

Cystic fibrosis (CF) is associated with frequent pulmonary exacerbations and the need for novel antibiotics against antimicrobial resistance. Cefiderocol is a newly approved therapeutic option active against a variety of multidrug resistant (MDR) bacteria such as gram-negative species commonly encountered by CF patients. This review describes the potential role of cefiderocol against , , , and complex. Read More

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Regulation of Glycine Cleavage and Detoxification by a Highly Conserved Glycine Riboswitch in Burkholderia spp.

Curr Microbiol 2021 Jun 2. Epub 2021 Jun 2.

Department of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia.

The glycine riboswitch is a known regulatory element that is unique in having two aptamers that are joined by a linker region. In this study, we investigated a glycine riboswitch located in the 5' untranslated region of a glycine cleavage system homolog (gcvTHP) in Burkholderia spp. Structure prediction using the sequence generated a model with a glycine binding pocket composed of base-triple interactions (G62-A64-A86 and G65-U84-C85) that are supported by A/G minor interactions (A17-C60-G88 and G16-C61-G87, respectively) and two ribose-zipper motifs (C11-G12 interacting with A248-A247 and C153-U154 interacting with A79-A78) which had not been previously reported. Read More

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Genomic Analysis of a Novel Phage Infecting the Turkey Pathogen APEC O78 and Its Endolysin Activity.

Viruses 2021 May 31;13(6). Epub 2021 May 31.

Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

Due to the increasing spread of multidrug-resistant (MDR) bacteria, phage therapy is considered one of the most promising methods for addressing MDR bacteria. lives symbiotically in the intestines of humans and some animals, and most strains are beneficial in terms of maintaining a healthy digestive tract. However, some strains can cause serious zoonotic diseases, including diarrhea, pneumonia, urinary tract infections, and hemolytic uremic syndrome. Read More

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An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral Infection.

Pathogens 2021 May 18;10(5). Epub 2021 May 18.

Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA.

Non-typhoidal salmonellosis, caused by serovar Typhimurium is a common fecal-oral disease characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body aches, nausea, and vomiting. Increasing incidences of antibiotic resistant invasive non-typhoidal infections makes this a global threat requiring novel treatment strategies including next-generation vaccines. The goal of the current study was to formulate a novel vaccine platform against infection that could be delivered orally. Read More

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An Overview of Metabolic Activity, Beneficial and Pathogenic Aspects of Spp.

Metabolites 2021 May 17;11(5). Epub 2021 May 17.

School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.

is an important bacterial species which has different beneficial effects, such as promoting the plant growth, including rhizosphere competence for the secretion of allelochemicals, production of antibiotics, and siderophores. In addition, most of species have demonstrated promising biocontrol action against different phytopathogens for diverse crops. In particular, demonstrates significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites. Read More

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Pan-Genome Analysis Reveals Host-Specific Functional Divergences in .

Microorganisms 2021 May 22;9(6). Epub 2021 May 22.

Department of Integrated Biological Science, Pusan National University, Busan 46241, Korea.

has high versatility and adaptability to various ecological niches. Here, we constructed a pan-genome using 14 genome sequences of , which originate from different niches, including gladiolus, rice, humans, and nature. Functional roles of core and niche-associated genomes were investigated by pathway enrichment analyses. Read More

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Use of Recombinant Strains in Immunofluorescence Assays for Melioidosis Diagnosis.

Pathogens 2021 May 6;10(5). Epub 2021 May 6.

Center of Excellent Research for Melioidosis (CERM), School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.

is a Gram-negative bacterium and the causative agent of melioidosis in humans and animals in the tropics. The clinical manifestations of melioidosis are diverse, ranging from localized infections to whole-body sepsis. The effective serological method is crucial for the point-of-care diagnosis of melioidosis. Read More

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The profile of the soil microbiota in the Cerrado is influenced by land use.

Appl Microbiol Biotechnol 2021 Jun 1;105(11):4791-4803. Epub 2021 Jun 1.

Industrial Microbiology and Bioremediation Department, Universidade Federal do Rio de Janeiro (UFRJ), Caxias, Rio de Janeiro, Brazil.

Extensive areas of the Cerrado biome have been deforested by the rapid advance of agricultural frontiers, especially by agricultural monocultures, and cultivated pastures. The objective of this study was to characterize the soil microbial community of an environment without anthropogenic interference and to compare it with soybean soil and pasture areas. For that, metagenomic sequencing techniques of the 16S rRNA gene were employed. Read More

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The nitrate, and nitrite pathways and the dynamic changes in the bacterial communities during beet sugar processing.

J Sci Food Agric 2021 May 31. Epub 2021 May 31.

COFCO Sugar Co., Ltd., Key Laboratory of Quality & Safety Control for Sugar Crops and Tomato, Ministry of Agriculture of the PRC, Changji, 831100, China.

The bacterial community successions were surveyed during the processing stages of sugar production using high-throughput sequencing methods. Furthermore, the correlation between bacterial community and nitrate/nitrite content in beet sugar processing were investigated. In an analysis of the V3-V4 region of the 16S rDNA gene, 254122 effective sequences were obtained from samples that included sugar beet, cossettes, diffusion juice, second-phase diffusion juice, light juice, and thick juice. Read More

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Characterization of microbiota diversity of engorged ticks collected from dogs in China.

J Vet Sci 2021 May;22(3):e37

Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Background: Ticks are one of the most common external parasites in dogs, and are associated with the transmission of a number of major zoonoses, which result in serious harm to human health and even death. Also, the increasing number of pet dogs and pet owners in China has caused concern regarding human tick-borne illnesses. Accordingly, studies are needed to gain a complete understanding of the bacterial composition and diversity of the ticks that parasitize dogs. Read More

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RegAB Homolog of Burkholderia pseudomallei is the Master Regulator of Redox Control and involved in Virulence.

PLoS Pathog 2021 May 28;17(5):e1009604. Epub 2021 May 28.

Friedrich Loeffler Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.

Burkholderia pseudomallei, the etiological agent of melioidosis in humans and animals, often occupies environmental niches and infection sites characterized by limited concentrations of oxygen. Versatile genomic features enable this pathogen to maintain its physiology and virulence under hypoxia, but the crucial regulatory networks employed to switch from oxygen dependent respiration to alternative terminal electron acceptors (TEA) like nitrate, remains poorly understood. Here, we combined a Tn5 transposon mutagenesis screen and an anaerobic growth screen to identify a two-component signal transduction system with homology to RegAB. Read More

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Imaging manifestations of pulmonary melioidosis: a case series.

Radiologia 2021 May 24. Epub 2021 May 24.

Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia; Clínica Universitaria Colombia, Clínica Colsanitas, Bogotá, Colombia.

Melioidosis is an endemic disease in Southeast Asia and Oceania caused by the gram-negative bacillus Burkholderia pseudomallei. We studied 15 adult patients from Colombia with microbiologically diagnosed pulmonary melioidosis. We reviewed 15 chest X-rays and 10 chest computed tomography (CT) studies. Read More

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MicroRNA-146a inhibits autophagy to maintain the intracellular survival of Burkholderia pseudomallei by targeting LIPA.

Microb Pathog 2021 May 24:104969. Epub 2021 May 24.

Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Third Military Medical University (Army Medical University), Chongqing, PR China; Department of Respiratory, General Hospital of Center Theater of PLA, PLA's Health Service Scientific Research Plan, Wuhan, PR China. Electronic address:

Burkholderia pseudomallei is the etiological agent of melioidosis, which is an emerging infectious disease endemic to many tropical regions. Autophagy is an intrinsic cellular process that degrades cytoplasmic components and plays an important role in protecting the host against pathogens. Like many intracellular pathogens, B. Read More

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Biotechnological utilization: the role of Zea mays rhizospheric bacteria in ecosystem sustainability.

Appl Microbiol Biotechnol 2021 Jun 27;105(11):4487-4500. Epub 2021 May 27.

Food Security and Safety Niche, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, Mmabatho, 2735, South Africa.

Maize is an essential cereal crop and the third most essential food crop globally. The extensive dependence on pesticides and chemical fertilizers to control pests and increase crop yield, respectively, has generated an injurious impact on soil and animal health. Plant growth-promoting rhizobacteria (PGPR), which depict a broad array of bacteria inhabiting the root vicinity and root surface, have proven to be a better alternative. Read More

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[Diversity and community characteristics of organic phosphate-mineralizing bacteria in the sea area between Minjiang Estuary to Pingtan.]

Ying Yong Sheng Tai Xue Bao 2021 May;32(5):1863-1872

College of Environmental Science and Engineering, Fujian Normal University, Fuzhou 350007, China.

Microbial mineralization of organic phosphorus is an important component of marine phosphorus cycle. The research on organic phosphate-mineralizing bacteria (OPB) is helpful to reveal microbial driving mechanism of organic phosphorus mineralization in eutrophic sea area. The diversity and community characteristics of OPB were examined by Illumina high-throughput sequencing using the primer sets in the sea area between Minjiang Estuary to Pingtan in April (spring) and July (summer) 2019. Read More

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Melioidosis Presenting as Septic Arthritis: The Role of F-18 Fludeoxyglucose Positron Emission Tomography/Computed Tomography in Diagnosis and Management.

Indian J Nucl Med 2021 Jan-Mar;36(1):59-61. Epub 2021 Mar 4.

Department of Nuclear Medicine, Narayana Hrudayalaya, Bengaluru, Karnataka, India.

Melioidosis, caused by the soil saprophyte , is a great mimicker. With its wide variety of presentations which are often nonspecific, a good clinical suspicion is required for the timely diagnosis of the disease. F-18 fludeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) has a well-established role in the diagnosis and management of various infective diseases. Read More

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Understanding the complexity of disease-climate interactions for rice bacterial panicle blight under tropical conditions.

PLoS One 2021 26;16(5):e0252061. Epub 2021 May 26.

International Center for Tropical Agriculture (CIAT), Agrobiodiversity Research Area, Cali, Colombia.

Bacterial panicle blight (BPB) caused by Burkholderia glumae is one of the main concerns for rice production in the Americas since bacterial infection can interfere with the grain-filling process and under severe conditions can result in high sterility. B. glumae has been detected in several rice-growing areas of Colombia and other countries of Central and Andean regions in Latin America, although evidence of its involvement in decreasing yield under these conditions is lacking. Read More

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Computational and experimental analysis on the preferential selectivity of lipases for triglycerides in Licuri oil.

Bioprocess Biosyst Eng 2021 May 26. Epub 2021 May 26.

Tiradentes University, Av. Murilo Dantas 300, Farolândia, Aracaju, SE, 49032-490, Brazil.

In the present study, we demonstrated the use of molecular docking as an efficient in silico screening tool for lipase-triglyceride interactions. Computational simulations using the crystal structures from Burkholderia cepacia lipase (BCL), Thermomyces lanuginosus lipase (TLL), and pancreatic porcine lipase (PPL) were performed to elucidate the catalytic behavior with the majority triglycerides present in Licuri oil, as follows: caprilyl-dilauryl-glycerol (CyLaLa), capryl-dilauryl-glycerol (CaLaLa), capryl-lauryl-myristoyl-glycerol (CaLaM), and dilauryl-myristoyl-glycerol (LaLaM). The computational simulation results showed that BCL has the potential to preferentially catalyze the major triglycerides present in Licuri oil, demonstrating that CyLaLa, (≈25. Read More

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Simple plug-in synthetic step for the synthesis of (-)-camphor from renewable starting materials.

Chembiochem 2021 May 25. Epub 2021 May 25.

FhG-IGB: Fraunhofer-Institut fur Grenzflachen- und Bioverfahrenstechnik IGB, Bio-, Chemo- und Elektrokatalyse BioCAt, GERMANY.

Racemic camphor and isoborneol are readily available as industrial side products, whereas (1R)-camphor is available from natural sources. Optically pure (1S)-camphor, however, is much more difficult to obtain. The synthesis of racemic camphor from a-pinene proceeds via an intermediary racemic isobornyl ester, which is then hydrolysed and oxidized to give camphor. Read More

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