9,177 results match your criteria Burkholderia


Complete Genome Sequence of Burkholderia contaminans SK875, Isolated from the Respiratory Tract of a Pig in the Republic of Korea.

Microbiol Resour Announc 2020 Jul 2;9(27). Epub 2020 Jul 2.

Department of Animal Sciences and Technology, Konkuk University, Seoul, Republic of Korea

SK875 was isolated from the respiratory tract of a pig in the Republic of Korea. Here, we report the genome of SK875, which consists of three circular chromosomes and one plasmid of 8,596,045 bp with 7,727 genes. Read More

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http://dx.doi.org/10.1128/MRA.00642-20DOI Listing

Emergence of multidrug-resistant non-fermentative gram negative bacterial infection in hospitalized patients in a tertiary care center of Nepal.

BMC Res Notes 2020 Jul 2;13(1):319. Epub 2020 Jul 2.

Department of Clinical Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.

Objective: This study was designed for the characterization and establishment of antibiotic susceptibility profiles of non-fermentative gram negative bacteria isolated from hospitalized patients in a tertiary care hospital of Nepal.

Results: A total of 402 non-fermentative gram negative bacteria was isolated in 1486 culture-positive cases from 6216 different clinical samples obtained from hospitalized patients. Among total non-fermentative gram negative bacterial isolates, the highest number was recovered from specimens collected from lower respiratory tract infections (n = 173, 43. Read More

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http://dx.doi.org/10.1186/s13104-020-05163-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330544PMC

Environmental contamination alters the intestinal microbial community of the livebearer killifish .

Heliyon 2020 Jun 23;6(6):e04190. Epub 2020 Jun 23.

Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas - ICB, Campus Carreiros, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, 96203-900, Brazil.

Intestinal microbiota perform important functions for the health of fishes. Knowing the microbial composition and evaluating the possible effects caused by anthropogenic pollution in the intestinal microbiota of fish populations might represent an important step in defining microbial biomarkers for water pollution. This study evaluated the impact of environmental contamination on the gut microbiota of the livebearer killifish . Read More

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http://dx.doi.org/10.1016/j.heliyon.2020.e04190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322053PMC

tepR encoding a bacterial enhancer-binding protein orchestrates the virulence and interspecies competition of Burkholderia glumae through qsmR and a type VI secretion system.

Mol Plant Pathol 2020 Jul 1. Epub 2020 Jul 1.

Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA, USA.

The pathogenesis of the rice pathogenic bacterium Burkholderia glumae is under the tight regulation of the tofI/tofR quorum-sensing (QS) system. tepR, encoding a group I bacterial enhancer-binding protein, negatively regulates the production of toxoflavin, the phytotoxin acting as a major virulence factor in B. glumae. Read More

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http://dx.doi.org/10.1111/mpp.12947DOI Listing

Electronic State of the His/Tyr-Ligated Heme of BthA by Mössbauer and DFT Analysis.

Inorg Chem 2020 Jun 30. Epub 2020 Jun 30.

Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.

The BthA protein from the microorganism contains two hemes with axial His/OH and His/Tyr coordinations separated by the closest interheme distance of 14 Å. BthA has a similar structure and belongs to the same family of multiheme cytochrome peroxidases as MauG, which performs long-range oxidation of the partner protein methylamine dehydrogenase. Magnetic Mössbauer spectroscopy of the diferric state of BthA corroborates previous structural work identifying a high-spin (His/OH) peroxidatic heme and a low-spin (His/Tyr) electron transfer heme. Read More

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http://dx.doi.org/10.1021/acs.inorgchem.0c01349DOI Listing

Matrix-assisted laser desorption/ionization time-of-flight MS for the accurate identification of complex and in the clinical microbiology laboratory.

J Med Microbiol 2020 Jun 29. Epub 2020 Jun 29.

Division of Respiratory Medicine, Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada.

complex (Bcc) bacteria, currently consisting of 23 closely related species, and , can cause serious and difficult-to-treat infections in people with cystic fibrosis. Identifying bacteria to the species level is considered important for understanding epidemiology and infection control, and predicting clinical outcomes. Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF) is a rapid method recently introduced in clinical laboratories for bacterial species-level identification. Read More

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http://dx.doi.org/10.1099/jmm.0.001223DOI Listing

Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress.

Sci Rep 2020 Jun 26;10(1):10453. Epub 2020 Jun 26.

Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.

A bpss2242 gene, encoding a putative short-chain dehydrogenase/oxidoreductase (SDR) in Burkholderia pseudomallei, was identified and its expression was up-regulated by ten-fold when B. pseudomallei was cultured under high salt concentration. Previous study suggested that BPSS2242 plays important roles in adaptation to salt stress and pathogenesis; however, its biological functions are still unknown. Read More

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http://dx.doi.org/10.1038/s41598-020-67382-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320009PMC

Insect-Associated Bacteria Assemble the Antifungal Butenolide Gladiofungin by Non-Canonical Polyketide Chain Termination.

Angew Chem Int Ed Engl 2020 Jun 26. Epub 2020 Jun 26.

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Department of Biomolecular Chemistry, Beutenbergstr. 11a, 07745, Jena, GERMANY.

Genome mining of one of the protective symbionts ( Burkholderia gladioli ) of the invasive beetle Lagria villosa revealed a cryptic gene cluster coding for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore. Targeted gene inactivation, metabolic profiling and bioassays led to the discovery of the gladiofungins as yet overlooked components of the antimicrobial armory of the beetle symbiont, specifically against the entomopathogenic fungus Purpureocillium lilacinum . By mutational analyses, isotope labeling and in silico analyses of the modular polyketide synthase, we found that the rare butenolide moiety of gladiofungins derives from an unprecedented polyketide chain termination reaction involving a glycerol-derived C3 building block. Read More

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http://dx.doi.org/10.1002/anie.202005711DOI Listing

A study of Rose Bengal against a 2-keto-3-deoxy-d--octulosonate cytidylyltransferase as an antibiotic candidate.

J Enzyme Inhib Med Chem 2020 Dec;35(1):1414-1421

Graduate School of Pharmaceutical Sciences, Ewha Woman's University, Seoul, Republic of Korea.

Frequent occurrences of multi-drug resistance of pathogenic Gram-negative bacteria threaten human beings. The CMP-2-keto-3-deoxy-d--octulosonic acid biosynthesis pathway is one of the new targets for antibiotic design. 2-Keto-3-deoxy-d--octulosonate cytidylyltransferase (KdsB) is the key enzyme in this pathway. Read More

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http://dx.doi.org/10.1080/14756366.2020.1751150DOI Listing
December 2020

Multi-drug resistant keratitis following laser keratomileusis complicated by interface fluid syndrome.

Indian J Ophthalmol 2020 Jul;68(7):1443-1444

Cornea and Anterior Segment, LV Prasad Eye Institute, GMR Varalakshmi Campus, Visakhapatnam, Andhra Pradesh, India.

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http://dx.doi.org/10.4103/ijo.IJO_1633_19DOI Listing

Plant growth-promoting bacteria isolated from wild legume nodules and nodules of Phaseolus vulgaris L. trap plants in central and southern Mexico.

Microbiol Res 2020 Jun 13;239:126522. Epub 2020 Jun 13.

Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, C.P. 11340, Mexico City, Mexico. Electronic address:

Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene sequence analysis, tested for plant growth-promoting (PGP) activities and the production of antimicrobial compounds, and analyzed for potential nodulation by amplifying the nodC gene. Read More

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http://dx.doi.org/10.1016/j.micres.2020.126522DOI Listing

Serological Survey of Humans Exposed to -Infected Equids: A Public Health Approach.

Asia Pac J Public Health 2020 Jun 25:1010539520930500. Epub 2020 Jun 25.

Indian Council of Agricultural Research, National Research Centre on Equines, Hisar, Haryana, India.

Glanders is a fatal bacterial infection of equids caused by . The infection can be transmitted to humans through prolonged direct contact with glanderous equids. Recently, reemergence of equine glanders has been reported in many countries. Read More

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http://dx.doi.org/10.1177/1010539520930500DOI Listing

Management and outcomes of Burkholderia cepacia complex bacteremia in patients without cystic fibrosis: a retrospective observational study.

Eur J Clin Microbiol Infect Dis 2020 Jun 25. Epub 2020 Jun 25.

Department of Pathology, Seoul National University Boramae Hospital, Seoul National University College of Medicine, 20 Boramae-ro 5-gil, Dongjak-gu, Seoul, 07061, Republic of Korea.

Burkholderia cepacia complex (BCC) is an emerging pathogen of nosocomial infection in chronic or critically ill patients without cystic fibrosis (CF). The objective was to evaluate the management and outcomes of BCC bacteremia in patients without CF. We conducted a retrospective study of non-CF adult patients with BCC bacteremia between January 1997 and December 2016 at 4 tertiary hospitals in South Korea. Read More

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http://dx.doi.org/10.1007/s10096-020-03960-2DOI Listing

Immune responses in beta-thalassaemia: heme oxygenase 1 reduces cytokine production and bactericidal activity of human leucocytes.

Sci Rep 2020 Jun 24;10(1):10297. Epub 2020 Jun 24.

The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.

Patients with beta-thalassaemia increase the risk of bacterial infections, particularly Burkholderia pseudomallei (Bp), the causative agent of melioidosis in Thailand. Impaired immune cell functions may be the cause of this susceptibility, but detailed mechanisms have not been defined. In this study, we observed impaired production of IFN-gamma and IL-10 by whole blood from beta-thalassaemia patients upon stimulation with a range of bacteria-derived stimuli. Read More

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http://dx.doi.org/10.1038/s41598-020-67346-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314746PMC
June 2020
5.078 Impact Factor

Clinical repercussions of Glanders (Burkholderia mallei infection) in a Brazilian child: a case report.

Rev Soc Bras Med Trop 2020 22;53:e20200054. Epub 2020 Jun 22.

Hospital de Urgência de Sergipe, Aracaju, SE, Brasil.

Glanders is a relatively unknown zoonotic disease caused by Burkholderia mallei. This bacterium affect solipeds and humans, and can be used as a biological warfare. Glanders is characterized as an occupational disease. Read More

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http://dx.doi.org/10.1590/0037-8682-0054-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310367PMC

Shotgun metagenomic sequencing data of sunflower rhizosphere microbial community in South Africa.

Data Brief 2020 Aug 11;31:105831. Epub 2020 Jun 11.

Food Security and Safety Niche, Faculty of Natural and Agricultural Sciences, Private Mail Bag X2046, North-West University, South Africa.

This dataset presents shotgun metagenomic sequencing of sunflower rhizosphere microbiome in Bloemhof, South Africa. Data were collected to decipher the structure and function in the sunflower microbial community. Illumina HiSeq platform using next generation sequencing of the DNA was carried out. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305406PMC

Open database searching enables the identification and comparison of bacterial glycoproteomes without defining glycan compositions prior to searching.

Mol Cell Proteomics 2020 Jun 23. Epub 2020 Jun 23.

Department of Microbiology and Immunology, University of Melbourne, Australia

Mass spectrometry has become an indispensable tool for the characterisation of glycosylation across biological systems. Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind. While glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not. Read More

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http://dx.doi.org/10.1074/mcp.TIR120.002100DOI Listing

Development of a DNA microarray assay for rapid detection of fifteen bacterial pathogens in pneumonia.

BMC Microbiol 2020 Jun 23;20(1):177. Epub 2020 Jun 23.

Department of Pulmonary & Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.

Background: The rapid identification of pathogenic bacteria is important for determining an appropriate antimicrobial therapy for pneumonia, but traditional bacterial culture is time-consuming and labourious. The aim of this study was to develop and evaluate a DNA microarray assay for the simultaneous detection of fifteen bacterial species directly from respiratory tract specimens in patients with pneumonia. These species included Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Mycoplasma pneumoniae, Enterococcus faecalis, Enterococcus faecium, Enterobacter cloacae, Stenotrophomonas maltophilia, Burkholderia cepacia, Legionella pneumophila and Chlamydia pneumoniae. Read More

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http://dx.doi.org/10.1186/s12866-020-01842-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310556PMC

Bacterial-induced cell fusion is a danger signal triggering cGAS-STING pathway via micronuclei formation.

Proc Natl Acad Sci U S A 2020 Jun 22. Epub 2020 Jun 22.

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore;

is the causative agent of melioidosis, an infectious disease in the tropics and subtropics with high morbidity and mortality. The facultative intracellular bacterium induces host cell fusion through its type VI secretion system 5 (T6SS5) as an important part of its pathogenesis in mammalian hosts. This allows it to spread intercellularly without encountering extracellular host defenses. Read More

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http://dx.doi.org/10.1073/pnas.2006908117DOI Listing

Investigation of Burkholderia cepacia complex bacteremia outbreak in a neonatal intensive care unit: a case series.

J Med Case Rep 2020 Jun 23;14(1):76. Epub 2020 Jun 23.

Department of Microbiology, SGT University, Gurugram, Haryana, India.

Introduction: Burkholderia cepacia complex is a ubiquitous organism with a high virulence potential. It is found most commonly in moist environments. Hospital outbreaks have been reported from diverse sources such as contaminated faucets, nebulizers, disinfectant solutions, multidose antibiotic vials, tap water, bottled water, nasal sprays, and ultrasound gels. Read More

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http://dx.doi.org/10.1186/s13256-020-02415-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308110PMC

High clinical impact of rapid susceptibility testing on CHROMID ESBL medium directly from swabs.

Ann Transl Med 2020 May;8(9):604

Department of Microbiology, School of Medicine, University of Granada-Instituto de Investigación Biosanitaria, Granada, Spain.

Background: Antibiotic resistance is a serious public health challenge exacerbated by the widespread use of β-lactam and glycopeptide antibiotics. The identification of resistances is crucial, and CHROMID ESBL medium has been developed to detect enterobacteria with extended-spectrum β-lactamases (ESBL). The objective of this study was to evaluate the potential of this medium to detect other types of resistant bacteria. Read More

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http://dx.doi.org/10.21037/atm.2020.02.158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290529PMC

Parotitis due to Burkholderia cepacia Infection after Autologous Hematopoietic Stem Cell Transplantation.

Transpl Infect Dis 2020 Jun 21:e13374. Epub 2020 Jun 21.

Department of Laboratory, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.

Burkholderia cepacia predominantly causes opportunistic infections in hospitalized and immunocompromised patients such as patients with cystic fibrosis, cancer, or human immunodeficiency virus (HIV). Nonetheless, Burkholderia cepacia is infrequently reported to cause infection in hematopoietic stem cell transplantation (HSCT) recipients. Herein, we report a rare case of suppurative parotitis in a 31-year-old patient with T-cell lymphoblastic lymphoma (T-LBL) who underwent auto-HSCT. Read More

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http://dx.doi.org/10.1111/tid.13374DOI Listing

Antimicrobial resistance: Concerns of healthcare providers and people with CF.

J Cyst Fibros 2020 Jun 17. Epub 2020 Jun 17.

Department of Pediatrics, University of North Carolina, Chapel Hill, NC, USA. Electronic address:

Background: Chronic lung infections and their treatment pose risks for the development of antimicrobial resistance (AMR) in people with cystic fibrosis (PWCF). In this study, we evaluated the attitudes of healthcare providers' (HCP) and PWCF or their parents' toward AMR within the international CF community.

Methods: HCP and PWCF identified through listservs and CF-related organizations were asked to complete an AMR centered survey, with additional questions on antimicrobial stewardship (AMS) for HCP. Read More

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http://dx.doi.org/10.1016/j.jcf.2020.05.009DOI Listing

NMR-based metabolic profiling to follow the production of anti-phytopathogenic compounds in the culture of the marine strain Streptomyces sp. PNM-9.

Microbiol Res 2020 Jun 3;239:126507. Epub 2020 Jun 3.

Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera 30 No. 45-03, Edificio de Química of 427, Bogotá, 111321, Colombia. Electronic address:

Actinobacteria are the major source of bioactive secondary metabolites and are featured in the search for antimicrobial compounds. We used nuclear magnetic resonance (RMN)-metabolic profiling and multivariate data analysis (MVDA) to correlate the metabolites' production of Streptomyces sp. PNM-9 from the algae Dictyota sp. Read More

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http://dx.doi.org/10.1016/j.micres.2020.126507DOI Listing

In vitro and in planta antagonistic effects of plant growth promoting rhizobacteria consortium against soilborne plant pathogens of Solanum tuberosum and S. lycopersicum.

FEMS Microbiol Lett 2020 Jun 20. Epub 2020 Jun 20.

Potatoes (Solanum tuberosum L.) and tomatoes (Solanum lycopersicum L.), among the main crops belonging to the Solanaceae family, are attacked by several pathogens. Read More

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http://dx.doi.org/10.1093/femsle/fnaa099DOI Listing

Phenotypic characterization of trimeric autotransporter adhesin-defective bcaC mutant of Burkholderia cenocepacia: cross-talk towards the histidine kinase BCAM0218.

Microbes Infect 2020 Jun 15. Epub 2020 Jun 15.

iBB-Institute for Bioengineering and Biosciences, Biological Sciences Research Group, Av. Rovisco Pais 1, 1049-001, Lisbon, Portugal; Department of Bioengineering, Instituto Superior Técnico, University of Lisbon, Av. Rovisco Pais 1, 1049-001, Lisbon, Portugal. Electronic address:

Burkholderia cenocepacia is a virulent species belonging to the Burkholderia cepacia complex (Bcc) and one of the most problematic agents of chronic lung infection in cystic fibrosis patients. B. cenocepacia possesses a large panel of virulence traits that include trimeric autotransporter adhesins (TAAs). Read More

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http://dx.doi.org/10.1016/j.micinf.2020.05.018DOI Listing

A Case Report of Cystic Fibrosis Complicated by Burkholderia Cepacia and Cutaneous Vasculitis.

Cureus 2020 May 16;12(5):e8158. Epub 2020 May 16.

Pediatrics Section, Marie Curie Children's Emergency Hospital, Bucharest , ROU.

While the pulmonary and pancreatic involvement of cystic fibrosis (CF) is commonly described and therefore best studied, the cutaneous manifestations are frequently underdiagnosed, despite being important markers of disease severity. We report a case of antineutrophil cytoplasmic antibody-negative cutaneous vasculitis in a 15-year-old female CF patient in tandem with infection and subsequent colonization by Burkholderia cepacia complex (BCC). The flares of cutaneous vasculitis is associated closely with an infective exacerbation of CF and improved upon treatment of the infective exacerbation. Read More

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http://dx.doi.org/10.7759/cureus.8158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294859PMC

MELIOIDOSIS IN A BOTTLENOSE DOLPHIN () AFTER A HURRICANE IN THE CARIBBEAN ISLANDS.

J Zoo Wildl Med 2020 Jun;51(2):443-447

Center for Conservation Medicine and Ecosystem Health and Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.

Melioidosis is an emerging infectious disease of humans and animals caused by the bacterium and endemic in tropical regions, principally Southeast Asia and northern Australia. In September 2017, after Hurricane Maria impacted the Dolphin Discovery facility in the Federation of St. Kitts and Nevis, a juvenile male bottlenose dolphin () died within 96 hr of presenting with acute anorexia, lethargy, and respiratory distress. Read More

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http://dx.doi.org/10.1638/2019-0050DOI Listing

Soil Sickness in Aged Tea Plantation Is Associated With a Shift in Microbial Communities as a Result of Plant Polyphenol Accumulation in the Tea Gardens.

Front Plant Sci 2020 28;11:601. Epub 2020 May 28.

Key Laboratory of Fujian Province for Agroecological Process and Safety Monitoring, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China.

In conventional tea plantations, a large amount of pruned material returns to the soil surface, putting a high quantity of polyphenols into the soil. The accumulation of active allelochemicals in the tea rhizosphere and subsequent shift in beneficial microbes may be the cause of acidification, soil sickness, and regeneration problem, which may be attributed to hindrance of plant growth, development, and low yield in long-term monoculture tea plantation. However, the role of pruning leaf litter in soil sickness under consecutive tea monoculture is unclear. Read More

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http://dx.doi.org/10.3389/fpls.2020.00601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270330PMC

Whole genome shotgun sequencing of POPs degrading bacterial community dwelling tannery effluents and petrol contaminated soil.

Microbiol Res 2020 May 30;238:126504. Epub 2020 May 30.

Department of Biochemistry and Biotechnology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan. Electronic address:

The present study involved identification of genes which are present in the genome of native bacteria to make them effective tools for bioremediation of persistent organic pollutants (POPs). During this study, forty-one POPs (naphthalene, toluene and petrol) metabolizing bacteria were isolated from tannery effluents and petrol contaminated soil samples by successive enrichment culturing. The taxonomic diversity and gene repertoire conferring POPs degradation ability to the isolated bacterial community were studied through whole genome shotgun sequencing of DNA consortium. Read More

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http://dx.doi.org/10.1016/j.micres.2020.126504DOI Listing

Melioidosis in Gujarat: a neglected tropical disease.

Trop Doct 2020 Jun 12:49475520928219. Epub 2020 Jun 12.

Professor, Department of Microbiology, Kasturba Medical College, Manipal, and Coordinator, Center for Emerging and Tropical Diseases, Manipal Academy of Higher Education, Manipal, Karnataka, India.

We report what we believe is the first case in Gujarat of melioidosis in a 67-year-old man. Awareness of this disease is limited, especially in areas supposed not to be endemic. Read More

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http://dx.doi.org/10.1177/0049475520928219DOI Listing

Characterization of Complex Core Genome and the Underlying Recombination and Positive Selection.

Front Genet 2020 21;11:506. Epub 2020 May 21.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.

Recombination and positive selection are two key factors that play a vital role in pathogenic microorganisms' population adaptation and diversification. The complex (Bcc) represents bacterial species with high similarity, which can cause severe infections among cases suffering from the chronic granulomatous disorder and cystic fibrosis (CF). At present, no genome-wide study has been carried out focusing on investigating the core genome of Bcc associated with the two evolutionary forces. Read More

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http://dx.doi.org/10.3389/fgene.2020.00506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253759PMC

The Phages ΦE058 and ΦE067 Represent Distinct Prototypes of a New Subgroup of Temperate Myoviruses.

Front Microbiol 2020 27;11:1120. Epub 2020 May 27.

Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

and are highly pathogenic species which are closely related, but diverse regarding their prophage content. While temperate phages have not yet been isolated from , several phages of , and its non-pathogenic relative have been described. In this study we isolated two phages from and three phages from and determined their morphology, host range, and relationship. Read More

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http://dx.doi.org/10.3389/fmicb.2020.01120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266877PMC

Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community.

Front Microbiol 2020 12;11:750. Epub 2020 May 12.

Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, China.

The effects of nodulation properties of legumes on the rhizosphere bacterial community are still not clear. To determine the effects of nodulation phenotypes on bacterial communities in the rhizosphere of soybean plants, we performed high-throughput sequencing of the 16S rRNA gene to estimate the rhizosphere bacterial community of three soybean lines with different nodulation phenotypes grown in soil supplied with different levels of N fertilizer. The results revealed that both the soybean nodulation phenotypes and the N levels affected the rhizosphere bacteria community, but the nodulation phenotypes contributed more than the N-supply. Read More

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http://dx.doi.org/10.3389/fmicb.2020.00750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247815PMC

Evaluation of major rice cultivars for resistance to bacterial seedling rot caused by and identification of Japanese standard cultivars for resistance assessments.

Breed Sci 2020 Apr 19;70(2):221-230. Epub 2020 Mar 19.

Institute of Crop Science, NARO (National Agriculture and Food Research Organization), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.

causes bacterial seedling rot (BSR) and bacterial grain rot (BGR) in rice (), both of which are important diseases in Japan. We previously evaluated major Japanese cultivars for BGR resistance and selected standard cultivars for resistance assessments. Here, we assessed the BSR occurrence rate in cultivars from the World Rice Collection (WRC) and other sources and found wide variation in resistance. Read More

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http://dx.doi.org/10.1270/jsbbs.19117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272248PMC

Ultrasound-assisted production of biodiesel using engineered methanol tolerant Proteus vulgaris lipase immobilized on functionalized polysulfone beads.

Ultrason Sonochem 2020 Jun 3;68:105211. Epub 2020 Jun 3.

School of Chemistry, University of Melbourne, VIC 3010, Australia. Electronic address:

In the present study, Proteus vulgaris lipase (PVL) was engineered using directed evolution to increase methanol tolerance so that it would be more tolerant and efficient for harsh conditions employed in biodiesel synthesis, which is limiting their industrial use. The influence of ultrasound under different experimental conditions on the biodiesel conversion yield using methanolysis of non-edible neem oil was also emphasized. A special attention was also paid to the immobilization of lipase on Polysulfone (PS) beads and comparative studies with industrially used Burkholderia cepacia lipase. Read More

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http://dx.doi.org/10.1016/j.ultsonch.2020.105211DOI Listing

Rapid identification of melioidosis agent by an insulated isothermal PCR on a field-deployable device.

PeerJ 2020 27;8:e9238. Epub 2020 May 27.

Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Background: causes melioidosis, a serious illness that can be fatal if untreated or misdiagnosed. Culture from clinical specimens remains the gold standard but has low diagnostic sensitivity.

Method: In this study, we developed a rapid, sensitive and specific insulated isothermal Polymerase Chain Reaction (iiPCR) targeting gene ( Intracellular Motility A; BPSS1492) for the identification of A pair of novel primers: BimA(F) and BimA(R) together with a probe were designed and 121 clinical strains obtained from numerous clinical sources and 10 ATCC non-targeted strains were tested with iiPCR and qPCR in parallel. Read More

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http://dx.doi.org/10.7717/peerj.9238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261116PMC

Effects of Selected Functional Bacteria on Maize Growth and Nutrient Use Efficiency.

Microorganisms 2020 Jun 5;8(6). Epub 2020 Jun 5.

Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan Branch, Locked Bag No. 3, Sandakan 90509, Sabah, Malaysia.

Plant growth-promoting rhizobacteria (PGPR), which include isolates from genera , and have received attention due to their numerous plant growth-promoting mechanisms such as their ability to solubilize insoluble phosphates and nitrogen-fixation. However, there is a dearth of information on the potential plant growth-promoting effects of these three groups of bacteria on non-legumes such as maize. This study determined the influences of the aforementioned strains on soil properties, maize growth, nutrient uptake and nutrient use efficiency. Read More

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http://dx.doi.org/10.3390/microorganisms8060854DOI Listing

Tailoring a stable and recyclable nanobiocatalyst by immobilization of surfactant treated Burkholderia cepacia lipase on polyaniline nanofibers for biocatalytic application.

Int J Biol Macromol 2020 Jun 5;161:573-586. Epub 2020 Jun 5.

Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, S.A.S. Nagar 160062, Punjab, India. Electronic address:

Polyaniline nanofibers were synthesized by the oxidative polymerization of aniline. Surfactant treated lipase from Burkholdaria cepacia was immobilized on these polyaniline nanofibers by adsorption. The activity of immobilized preparation was six times higher than that of free lipase with an enhanced dispersion in organic solvents. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.002DOI Listing

Bacterial pathogens from lower respiratory tract infections: A study from Western Rajasthan.

J Family Med Prim Care 2020 Mar 26;9(3):1407-1412. Epub 2020 Mar 26.

Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India.

Respiratory tract infections are the most common diseases that are associated with social burden for the patient. Western Rajasthan has cases of Cystic fibrosis due to migrant population. The dry and dusty environment has led to prevalence of silicosis and COPD. Read More

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http://dx.doi.org/10.4103/jfmpc.jfmpc_994_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266181PMC

Polymicrobial community-acquired and bacteremia: opportunistic infections with similar risk factors in northern Australia.

IDCases 2020 23;21:e00833. Epub 2020 May 23.

Department of Medicine, Cairns Hospital, Cairns, QLD 4870, PO Box 902, Australia.

We report the case of a 61-year-old man from northern Australia with concurrent community-onset complex and bacteremia presenting as severe tropical pneumonia requiring intensive care unit support. The pneumonia was complicated by L3/4 discitis and vertebral osteomyelitis presumed to be due to melioidosis. His risk factors included chronic lung disease and immunosuppression with etanercept. Read More

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http://dx.doi.org/10.1016/j.idcr.2020.e00833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264049PMC

Systemic Melioidosis With Ruptured Splenic Abscess.

Cureus 2020 May 4;12(5):e7956. Epub 2020 May 4.

Surgery, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IND.

Melioidosis is a severe systemic disease caused by the bacterium , commonly found in soil, ground water, and ponds of endemic regions. It is transmitted to humans via percutaneous inoculation while working in these areas without protective clothing and footwear giving rise to the disease which has a high case fatality rate. It has a wide range of clinical manifestations, varying from asymptomatic infection to localized abscess formation to fulminating disease with multiple organ involvement and even death. Read More

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http://dx.doi.org/10.7759/cureus.7956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270831PMC

Performance of Burkholderia multivorans CCA53 for ethyl red degradation.

J Gen Appl Microbiol 2020 Jun 5. Epub 2020 Jun 5.

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST).

The discharge of industrial dyes and their breakdown products is often environmentally harmful. Here, we describe a biodegradation method using Burkholderia multivorans CCA53, which exhibits a capacity to degrade azo dyes, particularly ethyl red. Under optimized culture conditions, 100 μM ethyl red was degraded more than 99% after incubation for 8 h. Read More

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http://dx.doi.org/10.2323/jgam.2019.11.001DOI Listing

Greener approach for synthesis of N,N,N-trimethyl chitosan (TMC) using ternary deep eutectic solvents (TDESs).

Carbohydr Res 2020 Jul 15;493:108033. Epub 2020 May 15.

Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400 019, India. Electronic address:

N,N,N-trimethyl chitosan (TMC), quaternized hydrophilic derivative of chitosan, has been projected to have wide applications in the pharmaceutical industry owing to its improved solubility at physiological conditions. However, the conventional synthesis of TMC involves toxic organic agents, which complicates its use for biological applications. Moreover, these reactions result into unwanted O-methylation and scission of the parent polymer. Read More

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http://dx.doi.org/10.1016/j.carres.2020.108033DOI Listing

Needs assessment for novel Gram-negative antibiotics in US hospitals: a retrospective cohort study.

Lancet Infect Dis 2020 Jun 4. Epub 2020 Jun 4.

Critical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, MD, USA.

Background: Evidence-based needs assessments for novel antibiotics against highly-resistant Gram-negative infections (GNIs) are scarce. We aimed to use real-world data from an electronic health record repository to identify treatment opportunities in US hospitals for GNIs resistant to all first-line drugs.

Methods: For this retrospective cohort study, population estimates with an unmet need for novel Gram-negative antibiotics were quantified using the Cerner Health Facts database (2009-15), aggregating episodes of infection in US hospitals with pathogens displaying difficult-to-treat resistance (DTR; resistance to carbapenems, other β-lactams, and fluoroquinolones) and episodes involving empirical coverage with reserve drugs (colistin or polymyxin B and aminoglycosides). Read More

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http://dx.doi.org/10.1016/S1473-3099(20)30153-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272178PMC

Talus osteomyelitis: An uncommon manifestation of melioidosis.

Int J Infect Dis 2020 Jun 4;97:94-95. Epub 2020 Jun 4.

Department of Diagnostic Imaging, National University Health System, 1E Kent Ridge Road, Level 12, Singapore 119228, Singapore; NUS Yong Loo Lin School of Medicine, NUHS Tower Block, 1E Kent Ridge Road, Level 11, Singapore 119228, Singapore.

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http://dx.doi.org/10.1016/j.ijid.2020.05.120DOI Listing

Identification, cloning and expression patterns of the genes related to phosphate solubilization in Burkholderia multivorans WS-FJ9 under different soluble phosphate levels.

AMB Express 2020 Jun 5;10(1):108. Epub 2020 Jun 5.

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.

As important plant growth-promoting rhizobacteria, phosphate-solubilizing bacteria (PSB) fix nitrogen, dissolve potassium, promote growth, improve the soil micro-environment, and enhance soil fertility. A high-efficiency PSB strain from the pine tree rhizosphere, Burkholderia multivorans WS-FJ9, was screened in our laboratory. In this study, using a Bio Screener fully automatic microbial growth curve meter to determine the growth of the WS-FJ9 strain in phosphate-removing medium, the growth and mineral phosphate solubilization of WS-FJ9 were measured by Mo-Sb colorimetry and organophosphate-solubilization plate assays. Read More

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http://dx.doi.org/10.1186/s13568-020-01032-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275115PMC

Winter warming in Alaska accelerates lignin decomposition contributed by Proteobacteria.

Microbiome 2020 Jun 5;8(1):84. Epub 2020 Jun 5.

Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, 73019, USA.

Background: In a warmer world, microbial decomposition of previously frozen organic carbon (C) is one of the most likely positive climate feedbacks of permafrost regions to the atmosphere. However, mechanistic understanding of microbial mediation on chemically recalcitrant C instability is limited; thus, it is crucial to identify and evaluate active decomposers of chemically recalcitrant C, which is essential for predicting C-cycle feedbacks and their relative strength of influence on climate change. Using stable isotope probing of the active layer of Arctic tundra soils after depleting soil labile C through a 975-day laboratory incubation, the identity of microbial decomposers of lignin and, their responses to warming were revealed. Read More

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http://dx.doi.org/10.1186/s40168-020-00838-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275452PMC

Management of initial colonisations with Burkholderia species in France, with retrospective analysis in five cystic fibrosis Centres: a pilot study.

BMC Pulm Med 2020 Jun 5;20(1):159. Epub 2020 Jun 5.

CRCM pédiatrique, Service de Pneumo-Allergologie pédiatrique, Hôpital des Enfants, Centre Hospitalier Universitaire de Toulouse, 330 avenue de Grande-Bretagne - TSA 40031, 31059, Toulouse cedex 9, France.

Background: Whereas Burkholderia infections are recognized to impair prognosis in cystic fibrosis (CF) patients, there is no recommendation to date for early eradication therapy. The aim of our study was to analyse the current management of initial colonisations with Burkholderia cepacia complex (BCC) or B. gladioli in French CF Centres and its impact on bacterial clearance and clinical outcome. Read More

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http://dx.doi.org/10.1186/s12890-020-01190-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275364PMC

Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis.

Med Microbiol Immunol 2020 Jun 3. Epub 2020 Jun 3.

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK.

In the original article, incorrect  figures were published with incorrect captions. The correct figures and captions are given below. Read More

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http://dx.doi.org/10.1007/s00430-020-00682-2DOI Listing