8,301 results match your criteria Burkholderia


The antibiotic action of methylarsenite is an emergent property of microbial communities.

Mol Microbiol 2018 Nov 22. Epub 2018 Nov 22.

Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, 33199, USA.

Arsenic is the most ubiquitous environmental toxin. Here, we demonstrate that bacteria have evolved the ability to use arsenic to gain a competitive advantage over other bacteria at least twice. Microbes generate toxic methylarsenite (MAs(III)) by methylation of arsenite (As(III)) or reduction of methylarsenate (MAs(V)). Read More

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November 2018

Pan-drug-resistant and biofilm-producing strain of : first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia.

Int Med Case Rep J 2018 9;11:319-323. Epub 2018 Nov 9.

Department of Microbiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia,

Melioidosis, an infectious disease caused by , has recently gained importance as an emerging infectious disease in Indonesia. Reports of this infection in Indonesia are limited, although cases have been reported in Makassar, South Sulawesi. We report a case of cutaneous melioidosis caused by pan-drug-resistant, moderate biofilm-producer strain of in a diabetic patient. Read More

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November 2018

Synthesis and Biological Evaluation of the Novel Growth Inhibitor Streptol Glucoside, Isolated from an Obligate Plant Symbiont.

Chemistry 2018 Dec 3. Epub 2018 Dec 3.

University of Zurich, Department of Chemistry, Winterthurerstrasse 190, CH-8057, Zurich, SWITZERLAND.

The plant Psychotria kirkii hosts an obligatory bacterial symbiont, Candidatus Burkholderia kirkii, in nodules on their leaves. Recently, a glucosylated derivative of (+)-streptol, (+)-streptol glucoside, was isolated from the nodulated leaves and was found to possess a plant growth inhibitory activity. To establish a structure activity relationship study, a convergent strategy was developed to obtain several pseudosugars from a single synthetic precursor. Read More

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December 2018

Development of analysis toolkit to visualize interaction energies generated by fragment molecular orbital calculations.

J Chem Inf Model 2018 Dec 5. Epub 2018 Dec 5.

In modern praxis, a knowledge-driven design of pharmaceutical compounds relies heavily on protein structure data. Nonetheless, quantification of the interaction between protein and ligand is of great importance in the theoretical evaluation of the ability of a pharmaceutical compound to comply with certain expectations. The FMO (fragment molecular orbital) method is handy in this regard. Read More

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December 2018

The afc antifungal activity cluster, which is under tight regulatory control of ShvR, is essential for transition from intracellular persistence of Burkholderia cenocepacia to acute pro-inflammatory infection.

PLoS Pathog 2018 Dec 4;14(12):e1007473. Epub 2018 Dec 4.

VBMI, INSERM, Université de Montpellier, Nîmes, France.

The opportunistic pathogen Burkholderia cenocepacia is particularly life-threatening for cystic fibrosis (CF) patients. Chronic lung infections with these bacteria can rapidly develop into fatal pulmonary necrosis and septicaemia. We have recently shown that macrophages are a critical site for replication of B. Read More

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December 2018
1 Read

Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei.

PLoS One 2018 30;13(11):e0208277. Epub 2018 Nov 30.

Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD, United States of America.

Mouse models have been essential to generate supporting data for the research of infectious diseases. Burkholderia pseudomallei, the etiological agent of melioidosis, has been studied using mouse models to investigate pathogenesis and efficacy of novel medical countermeasures to include both vaccines and therapeutics. Previous characterization of mouse models of melioidosis have demonstrated that BALB/c mice present with an acute infection, whereas C57BL/6 mice have shown a tendency to be more resistant to infection and may model chronic disease. Read More

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November 2018
1 Read

Phylogenetic diversity of rhizobia nodulating native Mimosa gymnas grown in a South Brazilian ecotone.

Mol Biol Rep 2018 Nov 28. Epub 2018 Nov 28.

Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Avenida General Carlos Cavalcanti, 4748 - Uvaranas, C.P. 6001, Ponta Grossa, PR, 84030-900, Brazil.

Floristic surveys performed in "Campos Gerais" (Paraná, Brazil), an ecotone of Mata Atlântica and Cerrado biomes, highlights the richness and relative abundance of the family Fabaceae and point out the diversity and endemism of Mimosa spp. Our study reports the genetic diversity of rhizobia isolated from root nodules of native/endemic Mimosa gymnas Barneby in three areas of Guartelá State Park, an important conservation unit of "Campos Gerais". Soils of the sample areas were characterized as sandy, acid, poor in nutrients and organic matter. Read More

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November 2018

MarR Family Transcription Factors from Species: Hidden Clues to Control of Virulence-Associated Genes.

Microbiol Mol Biol Rev 2019 Mar 28;83(1). Epub 2018 Nov 28.

Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA

SUMMARYSpecies within the genus exhibit remarkable phenotypic diversity. Genomic plasticity, including genome reduction and horizontal gene transfer, has been correlated with virulence traits in several species. However, the conservation of virulence genes in species otherwise considered to have limited potential for infection suggests that phenotypic diversity may not be explained solely on the basis of genetic diversity. Read More

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March 2019
2 Reads

Small Noncoding Regulatory RNAs from and Complex.

Int J Mol Sci 2018 Nov 27;19(12). Epub 2018 Nov 27.

iBB-Institute for Bioengineering and Biosciences, Departmento de Bioengenharia, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Torre Sul, Piso 6, 1049-001 Lisbon, Portugal.

Cystic fibrosis (CF) is the most life-limiting autosomal recessive disorder in Caucasians. CF is characterized by abnormal viscous secretions that impair the function of several tissues, with chronic bacterial airway infections representing the major cause of early decease of these patients. and bacteria from the complex (Bcc) are the leading pathogens of CF patients' airways. Read More

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November 2018
1 Read

A mouse model of binge alcohol consumption and Burkholderia infection.

PLoS One 2018 28;13(11):e0208061. Epub 2018 Nov 28.

Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America.

Background: Binge drinking, an increasingly common form of alcohol consumption, is associated with increased mortality and morbidity; yet, its effects on the immune system's ability to defend against infectious agents are poorly understood. Burkholderia pseudomallei, the causative agent of melioidosis can occur in healthy humans, yet binge alcohol use is progressively being recognized as a major risk factor. Although our previous studies demonstrated that binge alcohol exposure results in reduced alveolar macrophage function and increased Burkholderia virulence in vitro, no experimental studies have investigated the outcomes of binge alcohol on Burkholderia spp. Read More

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November 2018
1 Read

Burkholderia Cepacia Endophthalmitis: An Unusual Presentation.

J Ophthalmic Vis Res 2018 Oct-Dec;13(4):504-507

Department of Ophthalmology, Mission Nethralaya, Shillong, Meghalaya, India.

Purpose: To report three cases of endophthalmitis following uneventful phacoemulsification surgery.

Case Report: Three cases presented with blurriness of vision 2 to 3 weeks following phacoemulsification. Vitreous culture confirmed the diagnosis. Read More

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November 2018

The chirality of imazethapyr herbicide selectively affects the bacterial community in soybean field soil.

Environ Sci Pollut Res Int 2018 Nov 24. Epub 2018 Nov 24.

Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Natural Resources and Environmental Science, Zhejiang University, Hangzhou, 310058, China.

The chiral herbicide imazethapyr (IM) is frequently used to control weeds in soybean fields in northeast China. However, the impact of IM enantiomers on microbial communities in soil is still unknown. Genetic markers (16S rRNA V3-V4 regions) were used to characterize and evaluate the variation of the bacterial communities potentially effected by IM enantiomers. Read More

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November 2018
1 Read

In Vitro Activity of Cefiderocol, a Siderophore Cephalosporin, Against Gram-Negative Bacilli Isolated by Clinical Laboratories in North America and Europe in 2015-2016: SIDERO-WT-2015.

Int J Antimicrob Agents 2018 Nov 21. Epub 2018 Nov 21.

International Health Management Associates, Inc., Schaumburg, Illinois, USA.

Cefiderocol (S-649266) is a parenteral siderophore cephalosporin currently in Phase III of clinical development. In this study, we determined the in vitro susceptibility of a 2015-2016 collection of 8,954 clinical isolates of Gram-negative bacilli (GNB), provided by 100 clinical laboratories in North America and Europe, to cefiderocol and comparators using the Clinical and Laboratory Standards Institute broth microdilution method. Iron-depleted cation-adjusted Mueller-Hinton broth was used to test cefiderocol. Read More

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November 2018
7 Reads
4.300 Impact Factor

Variation of the Bacterial Community in the Rhizoplane Iron Plaque of the Wetland Plant .

Int J Environ Res Public Health 2018 Nov 22;15(12). Epub 2018 Nov 22.

School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.

The survival of wetland plants in iron, sulfur and heavy metals-rich mine tailing ponds has been commonly attributed to the iron plaque (IP) on the root surface that acts as a protective barrier. However, the contribution of bacteria potentially regulates the iron-sulfur cycle and heavy metal exclusion at the root surface has not been studied in depth, particularly from a microbial ecology perspective. In this study, a pot experiment using , a typical wetland plant, in non-polluted soil (NP) and tailing soil (T) was conducted. Read More

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November 2018
1 Read

Antibacterial properties of the pituitary adenylate cyclase-activating polypeptide: A new human antimicrobial peptide.

PLoS One 2018 21;13(11):e0207366. Epub 2018 Nov 21.

INRS-Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.

The Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), a polycationic, amphiphilic and helical neuropeptide, is well known for its neuroprotective actions and cell penetrating properties. In the present study, we evaluated the potent antibacterial property of PACAP38 and related analogs against various bacterial strains. Interestingly, PACAP38 and related analogs can inhibit the growth of various bacteria including Escherichia coli (JM109), Bacillus subtilis (PY79), and the pathogenic Burkholderia cenocepacia (J2315). Read More

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November 2018
2 Reads

The structure and function of the global citrus rhizosphere microbiome.

Nat Commun 2018 Nov 20;9(1):4894. Epub 2018 Nov 20.

Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, 33885, FL, USA.

Citrus is a globally important, perennial fruit crop whose rhizosphere microbiome is thought to play an important role in promoting citrus growth and health. Here, we report a comprehensive analysis of the structural and functional composition of the citrus rhizosphere microbiome. We use both amplicon and deep shotgun metagenomic sequencing of bulk soil and rhizosphere samples collected across distinct biogeographical regions from six continents. Read More

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November 2018
20 Reads
10.742 Impact Factor

Complete genome sequence of Burkholderia sp. JP2-270, a rhizosphere isolate of rice with antifungal activity against Rhizoctonia solani.

Microb Pathog 2018 Nov 17;127:1-6. Epub 2018 Nov 17.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Electronic address:

Burkholderia sp. JP2-270, a bacterium with a strong ability to inhibit the growth of Rhizoctonia solani, was isolated from the rhizosphere of rice. The phylogenetic analysis based on 16S rRNA gene revealed that JP2-270 belonged to Burkholderia cepacia complex. Read More

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November 2018
1 Read

Isolation of Burkholderia pseudomallei from a Pet Green Iguana, Belgium.

Emerg Infect Dis 2018 Dec;24(12):2331-2333

We isolated Burkholderia pseudomallei, the causative agent of melioidosis, from liver granulomas of a pet green iguana (Iguana iguana) in Belgium. This case highlights a risk for imported green iguanas acting as a reservoir for introduction of this high-threat, zoonotic pathogen into nonendemic regions. Read More

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December 2018
1 Read

Monensin Alters the Functional and Metabolomic Profile of Rumen Microbiota in Beef Cattle.

Animals (Basel) 2018 Nov 17;8(11). Epub 2018 Nov 17.

College of Agriculture, Communities, and the Environment, Kentucky State University, Frankfort, KY 40601, USA.

To identify differences in rumen function as a result of feeding monensin to beef cattle, rumen fluid metagenomics and metabolomics analyses were used to evaluate the functional attributes and metabolites of rumen microbiota in beef steers fed no or 200 mg/d of monensin. Eight rumen-fistulated steers were used in the study for a period of 53 days. Rumen fluid samples were collected on the last day of the experiment. Read More

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November 2018
12 Reads

Plant growth promoting rhizobacteria induced Cd tolerance in Lycopersicon esculentum through altered antioxidative defense expression.

Chemosphere 2018 Nov 2;217:463-474. Epub 2018 Nov 2.

Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh, 11451, Saudi Arabia; Department of Botany, S.P. College Srinagar, Jammu and Kashmir, India. Electronic address:

The present study was designed to determine the role of plant growth-promoting rhizobacteria (Pseudomonas aeruginosa &Burkholderia gladioli) in alleviating Cd stress in Lycopersicon esculentum. Cd concentration of 0.4 mM enhanced superoxide anions, MDA and HO by 136%, 378% and 137% that also caused nuclear and cell viability damage. Read More

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November 2018
2 Reads

Burkholderia mallei: The dynamics of networks and disease transmission.

Transbound Emerg Dis 2018 Nov 14. Epub 2018 Nov 14.

Department of Population Health and Pathobiology, College of Veterinary Medicine, Raleigh, North Carolina.

Glanders is a highly infectious zoonotic disease caused by Burkholderia mallei. The transmission of B. mallei occurs mainly by direct contact, and horses are the natural reservoir. Read More

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November 2018
4 Reads

Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying Burkholderia pseudomallei and Burkholderia thailandensis isolates.

Eur J Clin Microbiol Infect Dis 2018 Nov 13. Epub 2018 Nov 13.

Department of Laboratory Medicine, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing, 400042, People's Republic of China.

Since Burkholderia thailandensis is included in the reference spectra of the VITEK MS libraries rather than Burkholderia pseudomallei, B. pseudomallei cannot be correctly identified in the current version of VITEK MS. This study was undertaken to evaluate the utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with the VITEK MS plus system in the detection of B. Read More

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November 2018
5 Reads

Native valve dual pathogen endocarditis caused by and - a case report.

JMM Case Rep 2018 Sep 8;5(9):e005143. Epub 2018 Mar 8.

Armed Forces Institute of Pathology, National University of Medical Sciences, Islamabad, Pakistan.

Introduction: Infective endocarditis (IE) is an important clinical condition with significant morbidity and mortality among the affected population. A single etiological agent is identifiable in more than 90 % of the cases, however, polymicrobial endocarditis (PE) is a rare find, with a poor clinical outcome. Here we report a case of native valve dual pathogen endocarditis caused by and in an immunocompetent individual. Read More

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September 2018
7 Reads

Molecular analysis of clinical Burkholderia pseudomallei isolates from southwestern coastal region of India, using multi-locus sequence typing.

PLoS Negl Trop Dis 2018 Nov 12;12(11):e0006915. Epub 2018 Nov 12.

Microbiology Division, Defence Research & Development Establishment, Gwalior, Madhya Pradesh, India.

Background: The Gram-negative soil dwelling bacterium Burkholderia pseudomallei is the etiological agent of melioidosis. The disease is endemic in most parts of Southeast Asia and northern Australia. Over last few years, there has been an increase in number of melioidosis cases from India; however the disease epidemiology is less clearly understood. Read More

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November 2018
3 Reads

Genomics-guided discovery of a new and significantly better source of anticancer natural drug FK228.

Synth Syst Biotechnol 2018 Dec 5;3(4):268-274. Epub 2018 Nov 5.

UNT System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.

FK228 is an FDA-approved anticancer drug naturally produced by No. 968 up to 19 mg/L in a pilot industry-scale batch fermentation. Here we report a genomics-guided discovery of MSMB43 as a new and significantly better source of FK228. Read More

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December 2018
12 Reads

Enhancement of growth and salt tolerance of rice seedlings by ACC deaminase-producing Burkholderia sp. MTCC 12259.

J Plant Physiol 2018 Oct 12;231:434-442. Epub 2018 Oct 12.

Microbiology Laboratory, UGC Centre for Advanced Study, Department of Botany, Burdwan University, Burdwan, 713104, WB, India. Electronic address:

Increasing soil salinity is often associated with accelerated ethylene production in plants, leading to overall growth reduction. The salt-tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing PGPR may alleviate salt stress by reducing the production of stress ethylene. In this study, a salt-tolerant ACC deaminase-producing strain named P50 was isolated from a coastal rice field in Odisha, India, which enhanced the growth of rice seedlings under salt stress. Read More

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October 2018
9 Reads

The clinical characteristics of patients with sepsis in a tertiary referral hospital in Yangon, Myanmar.

Trans R Soc Trop Med Hyg 2018 Nov 9. Epub 2018 Nov 9.

Department of Medicine, Insein General Hospital, Min Gyi Road, Insein Township, Yangon, Myanmar.

Background: The clinical characteristics and course of patients hospitalised with sepsis in Myanmar and the responsible pathogens remain poorly defined.

Methods: We performed an observational study of adults admitted from the community to a tertiary referral hospital in Yangon with fever and dysfunction of at least two organ systems.

Results: The 120 patients had a median age of 47 y (interquartile range 28-63); 11 (9%) were human immunodeficiency virus positive. Read More

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November 2018
3 Reads

Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases.

World J Microbiol Biotechnol 2018 Nov 7;34(11):169. Epub 2018 Nov 7.

Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil.

The parameters that effect the synthesis of poly(styrene-co-divinylbenzene) magnetized with magnetite (STY-DVB-M) by polymerization emulsion were assessed in order to obtain magnetic beads to be used as matrix for lipase immobilization. The combined effect of polyvinyl alcohol (PVA) concentration and agitation was studied using response surface methodology. A 2 full-factorial design was employed for experimental design and analysis of the results. Read More

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November 2018
3 Reads

Proteomic Profiling of During Host Infection Using Bio-Orthogonal Noncanonical Amino Acid Tagging (BONCAT).

Front Cell Infect Microbiol 2018 23;8:370. Epub 2018 Oct 23.

Lawrence Livermore National Laboratory, Livermore, CA, United States.

and are the causative agents of melioidosis and glanders, respectively, and are often fatal to humans and animals. Owing to the high fatality rate, potential for spread by aerosolization, and the lack of efficacious therapeutics, and are considered biothreat agents of concern. In this study, we investigate the proteome of , a closely related surrogate for the two more virulent species, during infection of host cells, and compare to that of in culture. Read More

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October 2018
2 Reads

Next Generation Diagnostics for Melioidosis: Evaluation of a Prototype i-STAT Cartridge to Detect Burkholderia pseudomallei Biomarkers.

Clin Infect Dis 2018 Oct 31. Epub 2018 Oct 31.

Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.

Background: Infection with the gram-negative bacterium Burkholderia pseudomallei can result in melioidosis, a life-threatening disease that can be difficult to diagnose. Culture remains the gold standard for diagnosis but requires laboratory resources not available in many endemic regions. A lateral flow immunoassay has shown promise for POC diagnostics but suffers from low sensitivity when used on blood samples. Read More

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October 2018
5 Reads

Molecular Basis of Aqueous-like Activity of Lipase Treated with Glucose-Headed Surfactant in Organic Solvent.

J Phys Chem B 2018 Nov 14;122(47):10659-10668. Epub 2018 Nov 14.

Departments of Biological Sciences and Bioengineering , Lehigh University , 111 Research Drive , Bethlehem , Pennsylvania 18015 , United States.

Lipases are useful as catalysts, particularly for the kinetic resolution of racemic alcohols and esters. However, their industrial applications are limited by their poor activities in organic media. We recently found that a lipoprotein lipase from Burkholderia species displays dramatically enhanced activity in organic solvent if the protein is coated with glucose-headed surfactant (GHS). Read More

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November 2018
2 Reads

Living dangerously: Burkholderia pseudomallei modulates phagocyte cell death to survive.

Authors:
Teresa Krakauer

Med Hypotheses 2018 Dec 14;121:64-69. Epub 2018 Sep 14.

Department of Immunology, Molecular Translational Sciences Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, United States. Electronic address:

Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a major cause of sepsis and mortality in endemic regions of Southeast Asia and Northern Australia. As a facultative intracellular pathogen, B. pseudomallei produces virulence factors to evade innate host response and survive within host cells. Read More

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December 2018
2 Reads

Modulation of bacterial virulence and fitness by host glutathione.

Curr Opin Microbiol 2018 Nov 2;47:8-13. Epub 2018 Nov 2.

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Electronic address:

Glutathione is a low molecular weight thiol that is important for maintaining intracellular redox homeostasis. Some bacteria are able to import exogenous glutathione as a nutritional source and to counter oxidative stress. In cytosolic pathogens Burkholderia pseudomallei and Listeria monocytogenes, host glutathione regulates bacterial virulence. Read More

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November 2018
4 Reads

Burkholderia pseudomallei distribution in Australasia is linked to paleogeographic and anthropogenic history.

PLoS One 2018 5;13(11):e0206845. Epub 2018 Nov 5.

College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.

Burkholderia pseudomallei is the environmental bacillus that causes melioidosis; a disease clinically significant in Australia and Southeast Asia but emerging in tropical and sub-tropical regions around the globe. Previous studies have placed the ancestral population of the organism in Australia with a single lineage disseminated to Southeast Asia. We have previously characterized B. Read More

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November 2018

BpeB, a major resistance-nodulation-cell division transporter from Burkholderia cenocepacia: construct design, crystallization and preliminary structural analysis.

Acta Crystallogr F Struct Biol Commun 2018 Nov 16;74(Pt 11):710-716. Epub 2018 Oct 16.

Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.

Burkholderia cenocepacia is an opportunistic pathogen that infects cystic fibrosis patients, causing pneumonia and septicemia. B. cenocepacia has intrinsic antibiotic resistance against monobactams, aminoglycosides, chloramphenicol and fluoroquinolones that is contributed by a homologue of BpeB, which is a member of the resistance-nodulation-cell division (RND)-type multidrug-efflux transporters. Read More

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November 2018
1 Read

Profile of inhalable bacteria in PM at Mt. Tai, China: Abundance, community, and influence of air mass trajectories.

Ecotoxicol Environ Saf 2019 Jan 29;168:110-119. Epub 2018 Oct 29.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP, Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China; College of Geography and Environment, Shandong Normal University, Jinan 250100, China; Institut de Combustion, Aérothermique, Réactivité et Environnement, CNRS, 45071 Orléans Cedex 02, France.

Bacteria are ubiquitous in the near-surface atmosphere where they constitute an important component of aerosols with the potential to affect climate change, ecosystems, atmospheric process and human health. Limitation in tracking bacterial diversity accurately has thus far prevented the knowledge of airborne bacteria and their pathogenic properties. We performed a comprehensive assessment of bacterial abundance and diverse community in PM collected at Mt. Read More

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January 2019
1 Read

Isolation and prospection of diazotrophic rhizobacteria associated with sugarcane under organic management.

An Acad Bras Cienc 2018 Oct-Dec;90(4):3813-3829. Epub 2018 Oct 25.

Laboratório de Microbiologia Ambiental e Biotecnologia, Departamento de Biotecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Av. Universitária, s/n, 74605-050 Goiânia, GO, Brazil.

Microorganisms associated with organic management are essential in nutrient transformation and release for plant use. The present study aimed to isolate, identify and characterize plant growth promoting diazotrophic rhizobacteria associated with sugarcane under organic management. Rhizospheres of organic sugarcane varieties IAC 911099 and CTC4 were sampled and inoculated onto nitrogen free NFb and Burk media. Read More

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October 2018
5 Reads

Identification of Specific and Non-specific Inhibitors of Bacillus anthracis Type III Pantothenate Kinase (PanK).

ChemMedChem 2018 Oct 30. Epub 2018 Oct 30.

UNITED STATES.

Antibiotics with novel mechanisms are desperately needed to combat increasing rates of multi-drug resistant infections. Bacterial pantothenate kinase has emerged as a target of interest to cut off the biosynthesis of coenzyme A. In this work we report the results of an in vitro high-throughput screen of over 10,000 small molecules against Bacillus anthracis pantothenate kinase, as well as a follow up screen of hits against PanK isolated from Pseudomonas aeruginosa and Burkholderia cenocepacia. Read More

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October 2018
4 Reads

A Pair of Bacterial Siderophores Releases and Traps an Intercellular Signal Molecule: An Unusual Case of Natural Nitrone Bioconjugation.

Angew Chem Int Ed Engl 2018 Oct 30. Epub 2018 Oct 30.

Department of Biomolecular Chemistry, Leibniz Institute, for Natural Product Chemistry and Infection Biology (HKI), Beutenbergstrasse 11a, 07745, Jena, Germany.

In microbial interactions bacteria employ diverse molecules with specific functions, such as sensing the environment, communication with other microbes or hosts, and conferring virulence. Insights into the molecular basis of bacterial communication are thus of high relevance for ecology and medicine. Targeted gene activation and in vitro studies revealed that the cell-to-cell signaling molecule and disease mediator IQS (aeruginaldehyde) of the human pathogen Pseudomonas aeruginosa and related bacteria derives from the siderophore pyochelin. Read More

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October 2018
6 Reads

Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms.

Nat Commun 2018 Oct 30;9(1):4518. Epub 2018 Oct 30.

Laboratory of General Biochemistry and Physical Pharmacy, University of Ghent, Ghent, 9000, Belgium.

Hindered penetration of antibiotics through biofilms is one of the reasons for the alarming increase in bacterial tolerance to antibiotics. Here, we investigate the potential of laser-induced vapour nanobubbles (VNBs) formed around plasmonic nanoparticles to locally disturb biofilm integrity and improve antibiotics diffusion. Our results show that biofilms of both Gram-negative (Burkholderia multivorans, Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria can be loaded with cationic 70-nm gold nanoparticles and that subsequent laser illumination results in VNB formation inside the biofilms. Read More

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October 2018
17 Reads

Microplastic surface properties affect bacterial colonization in freshwater.

J Basic Microbiol 2018 Oct 29. Epub 2018 Oct 29.

Department of Biological Sciences, Kent State University, Kent, Ohio.

Microplastics are a global concern in aquatic ecology and are readily colonized by bacteria in the environment. There is a lack of information on bacterial colonization of eroded and un-eroded microplastics in freshwater. In this study, six types of microplastics were incubated for 8 weeks in microcosms with water from Lake Erie. Read More

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October 2018
6 Reads

The Roles of Two Genes in the Virulence and Stress Resistance of .

Plant Pathol J 2018 Oct 1;34(5):412-425. Epub 2018 Oct 1.

Department of Microbiology, Pusan National University, Busan 46241, Korea.

The Hfq protein is a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was conducted to elucidate the functions of two genes in , a causal agent of rice grain rot. Read More

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October 2018
2 Reads

Effect of on Postharvest Diseases and Induced Resistance of Strawberry Fruits.

Plant Pathol J 2018 Oct 1;34(5):403-411. Epub 2018 Oct 1.

College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi P.R., 030801, China.

This study takes strawberry-fruits as the test material and discusses the effect of B-1 on preventing postharvest diseases and inducing resistance-related substances in strawberry-fruits. Soaking and wound inoculating is performed to analyze the inhibitory effects of different treatment solutions on the gray mold of postharvest strawberry-fruits. The count of antagonistic bacteria colonies in the wound is found, and the dynamic growth of antagonistic bacteria and the pathogenic fungus is observed by electron microscopy. Read More

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October 2018
1 Read

Seed-born Infects Rice Seedling and Maintains Bacterial Population during Vegetative and Reproductive Growth Stage.

Plant Pathol J 2018 Oct 1;34(5):393-402. Epub 2018 Oct 1.

Instituto de Biotecnología, Universidad Nacional de Colombia, A.A 14-490, Bogotá D.C., Colombia.

Rice world production is affected due to the growing impact of diseases such as bacterial panicle blight, produced by . The pathogen-induced symptoms include seedling rot, grain rot and leaf-sheath browning in rice plants. It is currently recognized the entrance of this pathogen to the plant, from infected seeds and from environmental sources of the microorganism. Read More

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October 2018

Evaluation of major Japanese rice cultivars for resistance to bacterial grain rot caused by and identification of standard cultivars for resistance.

Breed Sci 2018 Sep 28;68(4):413-419. Epub 2018 Aug 28.

Institute of Crop Science, NARO (National Agriculture and Food Research Organization), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.

Bacterial grain rot (BGR), caused by the bacterial pathogen , is one of the most destructive rice () diseases in Japan; however, there are no BGR-resistant cultivars for use in Japan. We previously developed a cut-panicle inoculation method to assess the levels of BGR resistance in the World Rice Collection (WRC). Here, we evaluated major Japanese cultivars for BGR resistance and found that none showed "strong" or "medium to strong" resistance; most were categorized as "medium to weak". Read More

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September 2018

AR-13 reduces antibiotic-resistant bacterial burden in cystic fibrosis phagocytes and improves cystic fibrosis transmembrane conductance regulator function.

J Cyst Fibros 2018 Oct 23. Epub 2018 Oct 23.

Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, OH, United States; Division of Pulmonary Medicine, Nationwide Children's Hospital, Columbus, OH, United States. Electronic address:

Background: There are no effective treatments for Burkholderia cenocepacia in patients with cystic fibrosis (CF) due to bacterial multi-drug resistance and defective host killing. We demonstrated that decreased bacterial killing in CF is caused by reduced macrophage autophagy due to defective cystic fibrosis transmembrane conductance regulator (CFTR) function. AR-12 is a small molecule autophagy inducer that kills intracellular pathogens such as Francisella. Read More

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October 2018
4 Reads

Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells.

Environ Microbiol 2018 Oct 25. Epub 2018 Oct 25.

Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco, Madrid 28049, Spain.

The molecular mechanisms behind the mutagenic effect of reactive oxygen species (ROS) released by defective metabolization of xenobiotic 2,4-dinitrotoluene (DNT) by a still-evolving degradation pathway were studied. To this end, the genes required for biodegradation of DNT from Burkholderia cepacia R34 were implanted in Escherichia coli and the effect of catabolizing the nitroaromatic compound monitored with stress-related markers and reporters. Such a proxy of the naturally-occurring scenario faithfully recreated the known accumulation of ROS caused by faulty metabolism of DNT and the ensuing onset of an intense mutagenesis regime. Read More

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October 2018
5 Reads

Transcriptional analysis of the laccase-like gene from Burkholderia cepacia BNS and expression in Escherichia coli.

Appl Microbiol Biotechnol 2018 Oct 26. Epub 2018 Oct 26.

College of Natural Resources and Environment, Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Bacterial laccases have received considerable attention because of several advantages associated with the higher environmental stability of these enzymes compared with fungal laccases. In this study, a laccase-like gene from Burkholderia cepacia BNS was successfully cloned. This gene was found to encode a mature protein of 279 amino acids that exhibited laccase activity in dimer form. Read More

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October 2018
4 Reads

Deciphering the bacterial composition in the rhizosphere of Baphicacanthus cusia (NeeS) Bremek.

Sci Rep 2018 Oct 25;8(1):15831. Epub 2018 Oct 25.

School of Biomedical Sciences, Huaqiao University, 362021, Quanzhou, China.

Rhizobacteria is an important ingredient for growth and health of medicinal herbs, and synthesis of pharmacological effective substances from it. In this study, we investigated the community structure and composition of rhizobacteria in Baphicacanthus cusia (NeeS) Bremek via 16S rRNA amplicon sequencing. We obtained an average of 3,371 and 3,730 OTUs for bulk soil and rhizosphere soil samples respectively. Read More

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October 2018
3 Reads

Outbreak of complex infections associated with contaminated octenidine mouthwash solution, Germany, August to September 2018.

Euro Surveill 2018 Oct;23(42)

Institute of Medical Microbiology and Hygiene, Saarland University, Homburg/Saar, Germany.

Three German patients developed nosocomial pneumonia after cardiac surgery and had complex detected in respiratory specimens. Two patients died of septic multi-organ failure. Whole-genome sequencing detected genetically identical complex strains in patient samples, from a batch of octenidine mouthwash solution, which had been used for nursing care, as well as in samples obtained from the manufacturer during production. Read More

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October 2018
9 Reads