8,438 results match your criteria Burkholderia


Spatial and seasonal variation of the airborne microbiome in a rapidly developing city of China.

Sci Total Environ 2019 Feb 3;665:61-68. Epub 2019 Feb 3.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, China. Electronic address:

Exposure to airborne microbes (AM) can affect the human microbiome and has various consequences for human health. Investigating the profiles of AM and the potential bacterial pathogens within, along with the factors influencing their community, is pivotal for understanding the impact of AM on human health. In this study, we collected AM during spring and summer from 11 sites with various levels of urbanization in the city of Xiamen, China. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.01.367DOI Listing
February 2019

Direct, selective and ultrasensitive electrochemical biosensing of methyl parathion in vegetables using Burkholderia cepacia lipase@MOF nanofibers-based biosensor.

Talanta 2019 May 14;197:356-362. Epub 2019 Jan 14.

Department of Food Science and Engineering, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China. Electronic address:

Methyl parathion is one of the most widely used pesticides in agricultural practices. It caused accumulation of acetylcholine and over-stimulation of receptors in synapses which eventually led to damage of the nervous system. Present study developed a direct, sensitive, rapid and reliable method for methyl parathion residues detection in vegetable samples. Read More

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http://dx.doi.org/10.1016/j.talanta.2019.01.052DOI Listing

Disruption of quorum sensing and virulence in by a structural analogue of the -2-dodecenoic acid signal.

Appl Environ Microbiol 2019 Feb 15. Epub 2019 Feb 15.

School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou 510275, China

Quorum sensing (QS) signals are widely used by bacterial pathogens to control biological functions and virulence in response to changes in cell population densities. employs a molecular mechanism in which the -2-dodecenoic acid (named iffusible ignal actor, abbreviated as BDSF) QS system regulates -acyl homoserine lactone (AHL) signal production and virulence by modulating intracellular levels of cyclic diguanosine monophosphate (c-di-GMP). Thus, inhibition of BDSF signaling may offer a non-antibiotic-based therapeutic strategy against BDSF-regulated bacterial infections. Read More

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http://dx.doi.org/10.1128/AEM.00105-19DOI Listing
February 2019

[The development and application of reagents kit for detection RNA of the causative agents of melioidosis and glanders by transcription-based amplification (NASBA) in real-time.]

Klin Lab Diagn 2018 ;63(10):651-657

Volgograd Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare, 40013, Volgograd, Russia.

The polymorphism of clinical manifestations of melioidosis and glanders and their high mortality require improvement of diagnostics for detection of this agents. The perspectivity of development of transcription-based amplification real-time NASBA diagnostic kits is determined by high analytical sensitivity and the opportunity to perfom the verification of the results of other methods for pathogenic Burkholderia species detection. The fragment of 23S rRNA gene was selected as the target for development of real-time NASBA kit. Read More

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http://dx.doi.org/10.18821/0869-2084-2018-63-10-651-657DOI Listing
January 2018

A systematic review for variables to be collected in a transplant database for improving risk prediction.

Transplantation 2019 Feb 6. Epub 2019 Feb 6.

Evidence-Based Practice Research Program, Mayo Clinic, MN, USA.

Background: This systematic review was commissioned to identify new variables associated with transplant outcomes that are not currently collected by the Organ Procurement and Transplantation Network (OPTN).

Methods: We identified 81 unique studies including 1,193,410 patients with median follow-up of 36 months posttransplant, reporting 108 unique risk factors.

Results: Most risk factors (104) were recipient-related; few (4) were donor-related. Read More

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http://dx.doi.org/10.1097/TP.0000000000002652DOI Listing
February 2019

Head Tilt in Immunodeficient Mice Due to Contamination of Drinking Water by Burkholderia gladioli.

J Am Assoc Lab Anim Sci 2019 Feb 14. Epub 2019 Feb 14.

Immunodeficient mice in multiple holding rooms presented with head tilt, circling, spinning when picked up by the tail,dehydration, and lethargy. Burkholderia gladioli, a plant pathogen, was identified as the causative agent. Environmental testing revealed the presence of B. Read More

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http://dx.doi.org/10.30802/AALAS-JAALAS-18-000106DOI Listing
February 2019

Whole Genome Sequencing and Comparative Genomics Analyses of sp. XY-2, a New Species Capable of Biodegrade Tetracycline.

Front Microbiol 2019 29;10:33. Epub 2019 Jan 29.

School of Minerals Processing and Bioengineering, Central South University, Changsha, China.

Few bacteria are resistant to tetracycline and can even biodegrade tetracycline in the environment. In this study, we isolated a bacterium sp. XY-2, which could biodegrade 74% tetracycline at pH 7. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361800PMC
January 2019

Temporal proteomic profiling reveals changes that support Burkholderia biofilms.

Pathog Dis 2019 Feb 13. Epub 2019 Feb 13.

Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD 21201, USA.

Melioidosis associated with opportunistic pathogen Burkholderia pseudomallei imparts a huge medical burden in Southeast Asia and Australia. At present there is no available human vaccine that protects against B. pseudomallei infection and antibiotic treatments are limited particularly for drug-resistant strains and bacteria in biofilm forms. Read More

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http://dx.doi.org/10.1093/femspd/ftz005DOI Listing
February 2019

Can the imaging manifestations of melioidosis prognosticate the clinical outcome? A 6-year retrospective study.

Insights Imaging 2019 Feb 13;10(1):17. Epub 2019 Feb 13.

Department of Biostatistics, Christian Medical College Vellore, Tamil Nadu, 632004, India.

Objective: Melioidosis being an important cause of community-acquired sepsis, caused by Burkholderia pseudomallei in the tropical and subtropical countries, is often underreported or misinterpreted on imaging investigations. We aim to describe the spectrum of imaging manifestations of melioidosis and to evaluate its role in prognosticating clinical outcome, and look for association of specific organ involvement with risk factors.

Methods: From January 2011 to October 2017, retrospective analysis of imaging investigations of 189 consecutive patients with culture-proven melioidosis was performed. Read More

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http://dx.doi.org/10.1186/s13244-019-0708-8DOI Listing
February 2019

Melioidosis with a subdural collection - a case report.

BMC Infect Dis 2019 Feb 12;19(1):143. Epub 2019 Feb 12.

Department of Medicine, Faculty of Medicine, University of Kelaniya, Kelaniya, Sri Lanka.

Background: Melioidosis is an infection caused by Burkholderia pseudomallei, which is more prevalent in the tropics and leads to significant morbidity and mortality. It characteristically produces widespread caseous lesions and abscesses, and can present with varied clinical manifestations. Melioidosis involving the central nervous system is uncommon. Read More

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http://dx.doi.org/10.1186/s12879-019-3782-0DOI Listing
February 2019

Root-Associated Endophytic Bacterial Community Composition of from Four Representative Provinces in China.

Microorganisms 2019 Feb 11;7(2). Epub 2019 Feb 11.

National Engineering Research Center of Juncao, Fuzhou 350002, China.

, a source of bio-energy with high biomass production, is a species that contains high crude protein and will be useful for solving the shortage of forage grass after the implementation of "Green for Grain" project in the Loess plateau of Northern Shaanxi in 1999. Plants may receive benefits from endophytic bacteria, such as the enhancement of plant growth or the reduction of plant stress. However, the composition of the endophytic bacterial community associated with the roots of is poorly elucidated. Read More

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http://dx.doi.org/10.3390/microorganisms7020047DOI Listing
February 2019

Oxidative Pathways of Deoxyribose and Deoxyribonate Catabolism.

mSystems 2019 Jan-Feb;4(1). Epub 2019 Feb 5.

Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Using genome-wide mutant fitness assays in diverse bacteria, we identified novel oxidative pathways for the catabolism of 2-deoxy-d-ribose and 2-deoxy-d-ribonate. We propose that deoxyribose is oxidized to deoxyribonate, oxidized to ketodeoxyribonate, and cleaved to acetyl coenzyme A (acetyl-CoA) and glyceryl-CoA. We have genetic evidence for this pathway in three genera of bacteria, and we confirmed the oxidation of deoxyribose to ketodeoxyribonate . Read More

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http://dx.doi.org/10.1128/mSystems.00297-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365646PMC
February 2019

Human Tick-Borne Diseases in Australia.

Front Cell Infect Microbiol 2019 28;9. Epub 2019 Jan 28.

Neuroinflammation Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia.

There are 17 human-biting ticks known in Australia. The bites of , and can cause paralysis, inflammation, and severe local and systemic reactions in humans, respectively. Six ticks, including , and may transmit , or subsp. Read More

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http://dx.doi.org/10.3389/fcimb.2019.00003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360175PMC
January 2019
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Application of qPCR assays based on haloacids transporter gene dehp2 for discrimination of Burkholderia and Paraburkholderia.

BMC Microbiol 2019 Feb 11;19(1):36. Epub 2019 Feb 11.

Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

Background: A major facilitator superfamily transporter Dehp2 was recently shown to be playing an important role in transport and biodegradation of haloacids in Paraburkholderia caribensis MBA4, and Dehp2 is phylogenetically conserved in Burkholderia sensu lato.

Results: We designed both Burkholderia sensu stricto-specific and Paraburkholderia-specific qPCR assays based on dehp2 and 16S rRNA, and validated the qPCR assays in 12 bacterial strains. The qPCR assays could detect single species of Burkholderia sensu stricto or Paraburkholderia with high sensitivity and discriminate them in mixtures with high specificity over a wide dynamic range of relative concentrations. Read More

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http://dx.doi.org/10.1186/s12866-019-1411-0DOI Listing
February 2019

Response of soil bacterial community to bioaugmentation with a plant residue-immobilized bacterial consortium for crude oil removal.

Chemosphere 2019 Jan 25;222:831-838. Epub 2019 Jan 25.

Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, No.99, ShangDa Road, BaoShan District, Shanghai 200444, China.

Both the crude oil removal efficiency of the Eichhornia crassipes dried straw-immobilized bacterial consortium and shifts in soil bacterial community in response to the bioaugmentation strategy were unmasked in this study. After 30 days of bioremediation, total petroleum hydrocarbon in soil was determined and immobilized consortium showed a removal percentage (51.7%) better than either Eichhornia crassipes dried powder (37. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.01.133DOI Listing
January 2019

Comparative cytology, physiology and transcriptomics of Burkholderia insecticola in symbiosis with the bean bug Riptortus pedestris and in culture.

ISME J 2019 Feb 11. Epub 2019 Feb 11.

Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.

In the symbiosis of the bean bug Riptortus pedestris with Burkholderia insecticola, the bacteria occupy an exclusive niche in the insect midgut and favor insect development and reproduction. In order to understand how the symbiotic bacteria stably colonize the midgut crypts and which services they provide to the host, we compared the cytology, physiology, and transcriptomics of free-living and midgut-colonizing B. insecticola. Read More

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http://www.nature.com/articles/s41396-019-0361-8
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http://dx.doi.org/10.1038/s41396-019-0361-8DOI Listing
February 2019
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Misidentification of and Other Species From Pediatric Infections in Mexico.

Open Forum Infect Dis 2019 Feb 18;6(2):ofz008. Epub 2019 Jan 18.

Instituto Politecnico Nacional, Escuela Nacional de Ciencias Biológicas, Mexico City, Mexico.

and complex are poorly studied in Mexico. The genotypic analysis of 38 strains isolated from children with pneumonia were identified and showed that both groups were present in patients. From our results, it is plausible to suggest that new species are among the analyzed strains. Read More

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http://dx.doi.org/10.1093/ofid/ofz008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359878PMC
February 2019
1 Read

Evaluation of different molecular and phenotypic methods for identification of environmental Burkholderia cepacia complex.

World J Microbiol Biotechnol 2019 Feb 9;35(3):39. Epub 2019 Feb 9.

Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Av. do Café S/N. Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil.

The correct identification of different genera and bacterial species is essential, especially when these bacteria cause infections and appropriate therapies need to be chosen. Bacteria belonging to the Burkholderia cepacia complex are considered important opportunistic pathogens, causing different types of infections in immunocompromised, principally in patients with cystic fibrosis. Twenty-one isolates were obtained from different soil samples and identified by sequencing of 16S rRNA, 23S rRNA, recA gene, MLST and by VITEK 2 and MALDI-TOF MS systems. Read More

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http://dx.doi.org/10.1007/s11274-019-2614-0DOI Listing
February 2019
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Genome sequencing and traits analysis of Burkholderia strains reveal a promising biocontrol effect against grey mould disease in grapevine (Vitis vinifera L.).

World J Microbiol Biotechnol 2019 Feb 9;35(3):40. Epub 2019 Feb 9.

Unité de Recherche Résistance Induite et BioProtection des Plantes, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Université de Reims-Champagne-Ardenne, 51687, Reims Cedex 2, France.

Grey mould caused by Botrytis cinerea is among the most important disease affecting the production of grapevine worldwide. The high economical loss each year has led producers to become more dependent on chemical pesticides for protection. However, environmental impacts of the pesticides overuse have sparked crescent interest in developing alternative biocontrol methods. Read More

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http://dx.doi.org/10.1007/s11274-019-2613-1DOI Listing
February 2019

Feces from wild Triatoma dimidiata induces local inflammation and specific immune response in a murine model

Ann Parasitol 2018 ;64(4):367-377

Centro de Investigaciones Biomédicas, Universidad Autonoma de Campeche, Patrico Trueba s/n, Campeche 24090, Mexico

In endemic regions for Triatoma dimidiata the vector for Chagas disease, subjects can be in contact with insect`s feces several times through a lifetime. The triatomine’s digestive tract is colonized by diverse but few dominant genera of microorganisms. The immune responses to microbiota feces are poorly known in mammal hosts. Read More

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January 2018

Methylomic correlates of autophagy activity in cystic fibrosis.

J Cyst Fibros 2019 Feb 5. Epub 2019 Feb 5.

Department of Microbial Infection and Immunity, Infectious Diseases Institute, USA; The Ohio State University, Columbus, OH 43210, USA. Electronic address:

Autophagy is a highly regulated, biological process that provides energy during periods of stress and starvation. This conserved process also acts as a defense mechanism and clears microbes from the host cell. Autophagy is impaired in Cystic Fibrosis (CF) patients and CF mice, as their cells exhibit low expression levels of essential autophagy molecules. Read More

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http://dx.doi.org/10.1016/j.jcf.2019.01.011DOI Listing
February 2019
2 Reads

Inhibition of bacterial α-, β- and γ-class carbonic anhydrases with selenazoles incorporating benzenesulfonamide moieties.

J Enzyme Inhib Med Chem 2019 Dec;34(1):244-249

a Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche , Università degli Studi di Firenze , Florence , Italy.

A series of benzenesulfonamides incorporating selenazoles with diverse substitution patterns were investigated as inhibitors of six bacterial carbonic anhydrases (CAs, EC 4.2.1. Read More

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http://dx.doi.org/10.1080/14756366.2018.1547287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327980PMC
December 2019

Exploitation of the bilosome platform technology to formulate antibiotics and enhance efficacy of melioidosis treatments.

J Control Release 2019 Feb 4. Epub 2019 Feb 4.

Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK.

Burkholderia pseudomallei is a Gram-negative intracellular bacterium which is recalcitrant to antibiotic therapy. There also is currently no licensed vaccine for this potentially fatal pathogen, further highlighting the requirement for better therapeutics to treat the disease melioidosis. Here we use an oral delivery platform, the bilosome to entrap already- licensed antibiotics. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.02.002DOI Listing
February 2019

Inflammasomes, Autophagy, and Cell Death: The Trinity of Innate Host Defense against Intracellular Bacteria.

Authors:
Teresa Krakauer

Mediators Inflamm 2019 8;2019:2471215. Epub 2019 Jan 8.

Department of Immunology, Molecular and Translational Sciences Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.

Inflammasome activation is an innate host defense mechanism initiated upon sensing pathogens or danger in the cytosol. Both autophagy and cell death are cell autonomous processes important in development, as well as in host defense against intracellular bacteria. Inflammasome, autophagy, and cell death pathways can be activated by pathogens, pathogen-associated molecular patterns (PAMPs), cell stress, and host-derived damage-associated molecular patterns (DAMPs). Read More

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http://dx.doi.org/10.1155/2019/2471215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341260PMC
January 2019
1 Read

Microbiome dysbiosis is associated with disease duration and increased inflammatory gene expression in systemic sclerosis skin.

Arthritis Res Ther 2019 Feb 6;21(1):49. Epub 2019 Feb 6.

Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Background: Infectious agents have long been postulated to be disease triggers for systemic sclerosis (SSc), but a definitive link has not been found. Metagenomic analyses of high-throughput data allows for the unbiased identification of potential microbiome pathogens in skin biopsies of SSc patients and allows insight into the relationship with host gene expression.

Methods: We examined skin biopsies from a diverse cohort of 23 SSc patients (including lesional forearm and non-lesional back samples) by RNA-seq. Read More

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http://dx.doi.org/10.1186/s13075-019-1816-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366065PMC
February 2019
1 Read
3.753 Impact Factor

Inhibition of α-, β-, γ-, δ-, ζ- and η-class carbonic anhydrases from bacteria, fungi, algae, diatoms and protozoans with famotidine.

J Enzyme Inhib Med Chem 2019 Dec;34(1):644-650

a Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche , Università degli Studi di Firenze , Florence , Italy.

Famotidine, an antiulcer drug belonging to the H antagonists class of pharmacological agents, was recently shown to potently inhibit human (h) and bacterial carbonic anhydrases (CAs, EC 4.2.1. Read More

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http://dx.doi.org/10.1080/14756366.2019.1571273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366436PMC
December 2019
1 Read

Characterization of iron-metabolizing communities in soils contaminated by acid mine drainage from an abandoned coal mine in Southwest China.

Environ Sci Pollut Res Int 2019 Feb 6. Epub 2019 Feb 6.

Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-Environmental Science and Technology, 808 Tianyuan Road, Guangzhou, 510650, Guangdong, China.

Acid mine discharge (AMD) has been demonstrated to have significant impacts on microbial community composition in the surrounding soil environment. However, their effect on adjacent soil has not been extensively studied. In this study, microbial community composition of 20 AMD-contaminated soil samples collected from an abandoned coal mine along an AMD creek was characterized using high-throughput sequencing. Read More

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http://dx.doi.org/10.1007/s11356-019-04336-6DOI Listing
February 2019
1 Read

"Resurrecting old β-lactams": the potent inhibitory activity of temocillin against multi-drug resistant spp. isolates from the United States.

Antimicrob Agents Chemother 2019 Feb 4. Epub 2019 Feb 4.

Research Service, Louis Stokes Cleveland Department of Veterans Affairs, Cleveland, OH 44106

spp. are opportunistic human pathogens that infect persons with cystic fibrosis and the immunocompromised. spp. Read More

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http://dx.doi.org/10.1128/AAC.02315-18DOI Listing
February 2019

Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update.

Ann Clin Microbiol Antimicrob 2019 Feb 4;18(1). Epub 2019 Feb 4.

Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, 632004, India.

Bacteria belonging to the Burkholderia cepacia complex (Bcc) are among the most important pathogens isolated from cystic fibrosis (CF) patients and in hospital acquired infections (HAI). Accurate identification of Bcc is questionable by conventional biochemical methods. Clonal typing of Burkholderia is also limited due to the problem with identification. Read More

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http://dx.doi.org/10.1186/s12941-019-0306-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360774PMC
February 2019

Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF.

PLoS Biol 2019 Feb 4;17(2):e3000123. Epub 2019 Feb 4.

Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers, State University of New Jersey, Newark, New Jersey, United States of America.

The diffusible signal factors (DSFs) are a family of quorum-sensing autoinducers (AIs) produced and detected by numerous gram-negative bacteria. The DSF family AIs are fatty acids, differing in their acyl chain length, branching, and substitution but having in common a cis-2 double bond that is required for their activity. In both human and plant pathogens, DSFs regulate diverse phenotypes, including virulence factor expression, antibiotic resistance, and biofilm dispersal. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000123
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http://dx.doi.org/10.1371/journal.pbio.3000123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361424PMC
February 2019
2 Reads

Cefiderocol: A Siderophore Cephalosporin with Activity Against Carbapenem-Resistant and Multidrug-Resistant Gram-Negative Bacilli.

Drugs 2019 Feb 2. Epub 2019 Feb 2.

Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.

Cefiderocol is an injectable siderophore cephalosporin discovered and being developed by Shionogi & Co., Ltd., Japan. Read More

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http://dx.doi.org/10.1007/s40265-019-1055-2DOI Listing
February 2019
2 Reads

The Scourge of Antibiotic-resistant Infections in Cystic Fibrosis.

Trends Microbiol 2019 Jan 29. Epub 2019 Jan 29.

GeneCology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland, Australia; Sunshine Coast Health Institute, University of the Sunshine Coast, Birtinya, Queensland, Australia.

Bacterial infections are the primary cause of respiratory decline and mortality in cystic fibrosis (CF) patients. In a recent study, Diaz Caballero and colleagues [1] (PLoS Pathog. 2018;14:e1007453) catalogued the molecular adaptation of a decade-long Burkholderia multivorans infection in a Canadian CF patient, which evolved to become resistant towards multiple classes of antibiotics. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0966842X193001
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http://dx.doi.org/10.1016/j.tim.2019.01.003DOI Listing
January 2019
1 Read

Intestinal Morphologic and Microbiota Responses to Dietary spp. in a Broiler Chicken Model.

Front Physiol 2018 17;9:1968. Epub 2019 Jan 17.

College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.

Dietary inclusion of probiotic spp. beneficially affect the broiler chickens by balancing the properties of the indigenous microbiota causing better growth performance. The effects of three spp. Read More

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http://dx.doi.org/10.3389/fphys.2018.01968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344408PMC
January 2019
1 Read

[Comparative analysis of LAMP and Real Time PCR methods to detect pathogens of glanders and meliodosis.]

Klin Lab Diagn 2018 ;63(6):378-384

State Scientific Center of Applied Microbiology and Biotechnology, Obolensk, Russia.

Results of detection of Burkholderia mallei and Burkholderia pseudomallei DNA strains by LAMP (Loop-mediated Isothermal Amplification) and Real Time PCR are shown. It has been revealed that, in Real Time PCR, primers steadily detected DNA of those microorganism for the sequences of which they were designed. The above mentioned primers did not detect DNA of heterologous strains. Read More

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http://dx.doi.org/10.18821/0869-2084-2018-63-6-378-384DOI Listing
January 2018

Non-oral, aerobic, Gram-negative bacilli in the oral cavity of Thai HIV-positive patients on Highly-active anti-retrovirus therapy medication.

J Investig Clin Dent 2019 Jan 30:e12387. Epub 2019 Jan 30.

Department of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

In the present study, we identified and evaluated the antibiotic susceptibility of 96 independent, aerobic, Gram-negative bacillus isolates from 255 Thai HIV-positive adults who were on Highly-active anti-retrovirus therapy (HAART) medication. Another 46 isolates from HIV non-HAART individuals, vertically transmitted HIV-positive individuals, and non-HIV controls were included for comparison. A total of 103 strains were tested for antibiotic susceptibility using disc diffusion for screening and E-test for minimal inhibitory concentration determination, with special attention on extended-spectrum beta-lactamase (ESBL) isolates. Read More

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http://dx.doi.org/10.1111/jicd.12387DOI Listing
January 2019

Guild Composition of Root-Associated Bacteria Changes with Increased Soil Contamination.

Microb Ecol 2019 Jan 30. Epub 2019 Jan 30.

Department of Environmental Engineering, Technical University of Denmark, 2800, Lyngby, Denmark.

The interaction of plants and root-associated bacteria encourage the removal of soil contaminants. Engineers and scientists have looked at this phenomenon as a possible means of soil treatment (rhizodegradation). In this study, root-associated bacteria were isolated and selected for growth on a model soil contaminant: polycyclic aromatic hydrocarbons. Read More

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http://dx.doi.org/10.1007/s00248-019-01326-6DOI Listing
January 2019

Biological activities associated with the volatile compound 2,5-bis(1-methylethyl)-pyrazine.

FEMS Microbiol Lett 2019 Jan 29. Epub 2019 Jan 29.

Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), PO BOX 50, 6700 AB Wageningen, the Netherlands.

Pyrazines are 1,4- diazabenzene based volatile organic compounds and known for their broad-spectrum antimicrobial activity. In the present study we assessed the antimicrobial activity of 2,5-bis(1-methylethyl)-pyrazine, produced by Paenibacillus sp. AD87 during co-culture with Burkholderia sp. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/femsle/fnz023DOI Listing
January 2019
4 Reads

Persistent Bacteremia in A Filipino Immigrant to the United States: A Case Report.

Trop Med Infect Dis 2019 Jan 28;4(1). Epub 2019 Jan 28.

HSHS St. Elizabeth's Hospital, O'Fallon, IL 62269, USA.

Melioidosis is rare in the United States and endemic to Southeast Asia and Australia. Treatment includes an initial intensive phase of intravenous ceftazidime or meropenem monotherapy depending on severity. The following report describes a case of persistent bacteremia with ceftazidime failure and prolonged meropenem therapy on a ceftazidime-susceptible strain of . Read More

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http://dx.doi.org/10.3390/tropicalmed4010020DOI Listing
January 2019

The virulence function and regulation of the metalloprotease gene prtA in the plant pathogenic bacterium, Burkholderia glumae.

Mol Plant Microbe Interact 2019 Jan 29. Epub 2019 Jan 29.

Louisiana State University Agricultural Center, Plant Pathology and Crop Physiology , 302 Life Sciences Building , Louisiana State University , Baton Rouge, Louisiana, United States , 70803 ;

Bacterial panicle blight caused by Burkholderia glumae is a major bacterial disease of rice. Our preliminary RNA-seq study showed that a serine metalloprotease gene, prtA, is regulated in a similar manner to the genes for the biosynthesis and transport of toxoflavin, which is a known major virulence factor of B. glumae. Read More

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http://dx.doi.org/10.1094/MPMI-11-18-0312-RDOI Listing
January 2019
1 Read

: An Outbreak in the Peritoneal Dialysis Unit.

Perit Dial Int 2019 Jan-Feb;39(1):92-95

Department of Renal Medicine, Middlemore Hospital, Auckland, New Zealand.

is a ubiquitous, opportunistic, environmental gram-negative bacillus which most commonly affects cystic fibrosis and immunocompromised patients. Rarely, it can cause peritoneal dialysis (PD) exit-site infection (ESI). Information relating to predisposing factors, clinical course, and treatment options for ESIs is limited. Read More

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http://dx.doi.org/10.3747/pdi.2018.00095DOI Listing
January 2019

Burkholderia cepacia lipase immobilization for hydrolytic reactions and the kinetic resolution of the non-equimolar mixtures of isomeric alcohols.

Bioorg Chem 2019 Jan 25. Epub 2019 Jan 25.

Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland.

The major drawbacks of native lipase applications in processes occurring in water or in organic solvents include: difficulties in catalyst recycling, low activity and operational instability. The immobilization of Burkholderia cepacia lipase by adsorption or covalent binding onto 5 differently functionalized carriers (silica, acrylic, cellulose-based) was performed to overcome this problem. The optimization of the reaction preparation in water-rich media was based on the hydrolytic reactivity of the preparations, as well as the thermal, operational and storage stabilities. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.01.041DOI Listing
January 2019

[The laboratory diagnostic in patients with mucoviscidosis: A review.]

Klin Lab Diagn 2018 ;63(5):315-320

The Federal State Budget Educational Institution of Higher Education "The Samara State Medical university" of Minzdrav of Russia, 443099, Samara, Russia.

The mycobacterioses as a group of diseases conditioned by non-tuberculosis mycobacteria, acquire even greater significance for patients from various risk groups. The patients with cystic fibrosis along with patients with other genetic diseases, consist risk group of infection with non-tuberculosis mycobacteria. The diagnostic of mycobacterioses in patients with cystic fibrosis has a number of peculiarities both at the stage of processing clinical material and the stage of identification of separated microorganisms. Read More

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http://dx.doi.org/10.18821/0869-2084-2018-63-5-315-320DOI Listing
January 2018
4 Reads

Characteristics of Organic Acid Secretion Associated with the Interaction between WS-FJ9 and Poplar Root System.

Biomed Res Int 2018 31;2018:9619724. Epub 2018 Dec 31.

Academy of Agricultural Sciences of Lianyungang, Lianyungang, Jiangsu 222006, China.

The objective of this study was to investigate whether plant-bacteria interaction affects the secretion of organic acids by both organisms and to assess whether the production of IAA by the bacterium increases the secretion of organic acids by root exudates, and if the stress produced by low available phosphorus (P) affects the production of organic acids by bacteria, by roots, or by root exudates in presence of bacterial cultures. With this purpose, we used as a biological model poplar plants and one strain of able to solubilize P. High performance liquid chromatography was utilized to measure organic acids. Read More

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http://dx.doi.org/10.1155/2018/9619724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330825PMC
December 2018

The Type VI Secretion System 5: Composition, Regulation and Role in Virulence.

Front Microbiol 2018 10;9:3339. Epub 2019 Jan 10.

Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany.

The soil saprophyte and Tier I select agent can cause rapidly fatal infections in humans and animals. The capability of switching to an intracellular life cycle during infection appears to be a decisive trait of for causing disease. harbors multiple type VI secretion systems (T6SSs) orthologs of which are present in the surrogate organism . Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2018.03339
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http://dx.doi.org/10.3389/fmicb.2018.03339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335564PMC
January 2019
5 Reads

Key Players and Individualists of Cyclic-di-GMP Signaling in .

Front Microbiol 2018 10;9:3286. Epub 2019 Jan 10.

Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role of all 25 putative c-di-GMP metabolizing proteins of H111 with respect to motility, colony morphology, pellicle formation, biofilm formation, and virulence. Read More

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http://dx.doi.org/10.3389/fmicb.2018.03286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335245PMC
January 2019
1 Read

Antimicrobial susceptibility of Burkholderia pseudomallei isolates in the Northern Vietnam.

J Glob Antimicrob Resist 2019 Jan 24. Epub 2019 Jan 24.

Microbiology Department, Bach Mai hospital, Hanoi, Vietnam.

Objectives: Antimicrobial suceptibility pattern of clinical Burkholderia pseudomallei in Vietnam has not reported since the first publication in 2008. The present study aimed to determine the antimicrobial susceptibility pattern of B. pseudomallei isolated in a tertiary referral center in Hanoi from January 2012 to December 2017. Read More

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http://dx.doi.org/10.1016/j.jgar.2019.01.024DOI Listing
January 2019

[Multilocus sequence analysis, biofilm production, antibiotic susceptibility and synergy tests of Burkholderia species in patients with and without cystic fibrosis].

Mikrobiyol Bul 2019 Jan;53(1):22-36

Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey.

Burkholderia spp. emerged as important pathogens in the airways of immunocompromised humans, especially those with cystic fibrosis (CF). Failure of identification with conventional techniques, high intrinsic resistance to most antibiotics and biofilm formation can cause difficulties in the treatment of these infections. Read More

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http://dx.doi.org/10.5578/mb.67730DOI Listing
January 2019
3 Reads

Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier I Select Agents in Tissues.

Emerg Infect Dis 2019 May 17;25(5). Epub 2019 May 17.

For safety, Select Agents in tissues must be inactivated and viability tested before the tissue undergoes further processing and analysis. In response to the shipping of samples of "inactivated" Bacillus anthracis that inadvertently contained live spores to nonregulated entities and partners worldwide, the Federal Register now mandates in-house validation of inactivation procedures and standardization of viability testing to detect live organisms in samples containing Select Agents that have undergone an inactivation process. We tested and validated formaldehyde and glutaraldehyde inactivation procedures for animal tissues infected with virulent B. Read More

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http://dx.doi.org/10.3201/eid2505.180928DOI Listing
May 2019
1 Read

A selective genome-guided method for environmental Burkholderia isolation.

J Ind Microbiol Biotechnol 2019 Jan 24. Epub 2019 Jan 24.

Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada.

The genus Burkholderia is an emerging source of novel natural products chemistry, yet to date few methods exist for the selective isolation of strains of this genus from the environment. More broadly, tools to efficiently design selection media for any given genus would be of significant value to the natural products and microbiology communities. Using a modification of the recently published SMART protocol, we have developed a two-stage isolation protocol for strains from the genus Burkholderia. Read More

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http://dx.doi.org/10.1007/s10295-018-02121-xDOI Listing
January 2019

Melioidosis in the Torres Strait Islands, Australia: Exquisite Interplay between Pathogen, Host, and Environment.

Am J Trop Med Hyg 2019 Jan 21. Epub 2019 Jan 21.

Department of Medicine, Cairns Hospital, Cairns, Australia.

, a bacterium that lives in the soil of the tropics, causes the disease melioidosis. This retrospective study investigated the temporospatial epidemiology of the 49 laboratory-confirmed melioidosis cases in the Torres Straits Islands of tropical Australia between 1997 and 2017. An identifiable risk factor for the disease was present in 43/49 (88%) cases and in 35/36 (97%) cases with complete clinical data. Read More

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http://dx.doi.org/10.4269/ajtmh.18-0806DOI Listing
January 2019
1 Read