7,975 results match your criteria Bulletin Of Experimental Biology And Medicine[Journal]


Influence of Tumor Suppressor p53 Functioning on the Expression of Antioxidant System Genes under the Action of Cytotoxic Compounds.

Bull Exp Biol Med 2020 May 5;169(1):169-175. Epub 2020 Jun 5.

Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia.

The effect of inhibition of the tumor suppressor p53 on the antioxidant system genes expression under the influence of cytotoxic compounds of the platinum group was studied. It was found that the action of platinum(II) and platinum(IV) complexes induced accumulation of p53 protein with a maximum in 12 h, which was confirmed by an increase in the expression of the P21 gene, the target gene of the p53 protein. It was shown that the action of platinum complexes activated the expression of catalase and superoxide dismutase 2 genes. Read More

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http://dx.doi.org/10.1007/s10517-020-04844-3DOI Listing

Effects of Covalent Conjugates of Fullerene Derivatives with Xanthene Dyes on Activity of Ca-ATPase of the Sarcoplasmic Reticulum.

Bull Exp Biol Med 2020 May 4;169(1):89-94. Epub 2020 Jun 4.

Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia.

The effects of the newly synthesized covalent conjugates of water-soluble fullerene derivatives (WSFD) with xanthene dyes: polyanionic WSFD-fluorescein (1), polycationic WSFD-fluorescein (2), polyanionic WSFD-eosin (3), and polyanionic WSFD (4), polycationic WSFD (5), fluorescein (6) and eosin (7), on activity of the membrane-bound Ca-ATPase of the sarcoplasmic reticulum (SR Ca-ATPase) were studied. Compounds 1, 3, 4, 6, and 7 inhibit the hydrolytic function of the enzyme, the inhibition constants for these compounds are Ki=1.3×10 M (1), Ki=4. Read More

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http://dx.doi.org/10.1007/s10517-020-04831-8DOI Listing

Respiratory Characteristics of Rat Liver Mitochondria Depend on the Duration of Moderate Hypothermia.

Bull Exp Biol Med 2020 May 3;169(1):29-34. Epub 2020 Jun 3.

Department of Biochemistry and Biophysics, Dagestan State University, Makhachkala, Dagestan Republic, Russia.

The development of pathological or compensatory-and-adaptive reactions in homoeothermic animals during various periods of hypothermia can be caused by shifts in the respiratory functions of the mitochondria. Short-term hypothermia promoted an increase in the rates of the glutamate- and succinate-dependent respiration of mitochondria. Phosphorylation rate increased as well, while oxidative phosphorylation coefficient (P/O), respiratory control, and 2,4-DNP sensitivity depended. Read More

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http://dx.doi.org/10.1007/s10517-020-04817-6DOI Listing

Effects of Dynamic and Static Loads on the Concentration of Sodium and Potassium in Murine Skeletal Muscles.

Bull Exp Biol Med 2020 May 3;169(1):1-4. Epub 2020 Jun 3.

National Research Tomsk State University, Tomsk, Russia.

The effects of dynamic and static load on the intracellular concentrations of sodium [Formula: see text] and potassium (K) in m. soleus and m. biceps, respectively, were studied in mice. Read More

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http://dx.doi.org/10.1007/s10517-020-04811-yDOI Listing

Molecular Profiling of Follicular Variant of Papillary Thyroid Cancer.

Bull Exp Biol Med 2020 May 3;169(1):85-88. Epub 2020 Jun 3.

Cancer Research Institute, Tomsk National Research Medical Center, Tomsk, Russia.

The molecular features of the follicular variant of papillary thyroid cancer are closely related to the clinical behavior of the tumor and the prognosis of the disease. BRAF-V600E mutations in patients with a follicular variant of papillary thyroid cancer have not been identified; however, the majority of patients had T3-4N0M0 stage of the disease. Changes in the expression of transcription and growth factors and AKT/m-TOR signaling pathway components were detected. Read More

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http://dx.doi.org/10.1007/s10517-020-04830-9DOI Listing

Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.

Bull Exp Biol Med 2020 May 3;169(1):63-66. Epub 2020 Jun 3.

Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis. The mice were infected with mycobacteria tuberculosis 3 months prior to onset of treatment. The preparations under examinations were administered twice a week over 2 months. Read More

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http://dx.doi.org/10.1007/s10517-020-04825-6DOI Listing

rs2072580T>A Polymorphism in the Overlapping Promoter Regions of the SART3 and ISCU Genes Associated with the Risk of Breast Cancer.

Bull Exp Biol Med 2020 May 3;169(1):81-84. Epub 2020 Jun 3.

Federal Research Center Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.

We analyzed association of potentially regulatory polymorphisms (rs590352, rs11542583, rs3829202, rs207258, and rs4796672) with breast cancer. A significant association was found between this disease and rs2072580T>A (p=0.001) located in the overlapping promoter regions of the SART3 and ISCU genes. Read More

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http://dx.doi.org/10.1007/s10517-020-04829-2DOI Listing

Restoration of β-Adrenergic Signaling and Activity of Akt-Kinase and AMP-Activated Protein Kinase with Metformin in the Myocardium of Diabetic Rats.

Bull Exp Biol Med 2020 May 3;169(1):24-28. Epub 2020 Jun 3.

Laboratory of Molecular Endocrinology and Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Moscow, Russia.

We studied the effect of metformin (100 and 200 mg/kg/day, 4 weeks) on the adenylyl cyclasestimulating effects of β-agonists and relaxin in the myocardial membranes and on activities of Akt-kinase, an effector component of insulin signaling, and AMP-activated protein kinase (AMPK), a cellular energy sensor, in the myocardium of rats with type 2 diabetes mellitus induced by high-fat diet and streptozotocin. Metformin normalized the ratio of adenylyl cyclase effects of β- and β-agonists in the myocardial membranes, that is reduced in DM2, and restored phosphorylation of Akt-kinase by Ser473 and AMPK by Thr172 in the myocardium of diabetic rats. The effect of metformin in a dose of 200 mg/kg/day was more pronounced. Read More

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http://dx.doi.org/10.1007/s10517-020-04816-7DOI Listing

Comparative Analysis of Cytotoxic Activity of New Nitrosyl Iron-Sulfur Complexes in Human Tumor Cells In Vitro.

Bull Exp Biol Med 2020 May 3;169(1):130-136. Epub 2020 Jun 3.

Laboratory of Experimental Chemotherapy, N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.

We studied cytotoxic activity of new tetranitrosyl NO-generating binuclear iron-sulfur [Fe-S] complexes containing different ligands in the molecule against tumor cells in vitro. Cytotoxic activity of the most active complex with cysteamine (CysAm) was compared with that of antitumor drug cisplatin. Caspase activation and morphological changes in cells were visualized by fluorescence microscopy. Read More

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http://dx.doi.org/10.1007/s10517-020-04839-0DOI Listing

Intranasal Human Recombinant Modified Glucagon-Like Peptide-1: High Antihyperglycemic Activity and Duration of Action in Mice.

Bull Exp Biol Med 2020 May 3;169(1):53-56. Epub 2020 Jun 3.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center" Kurchatov Institute" (National Research Center Kurchatov Institute - GosNIIgenetika), Moscow, Russia.

The study compared effectiveness of intranasal administration of glypin (human recombinant modified glucagon-like peptide-1) and reference drug Victoza in BALB/c mice. The minimum effective dose of intranasal glypin was 0.5 mg/kg, and a 2-fold elevation of this dose increased the parameters of glypin activity up to the maximal levels. Read More

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http://dx.doi.org/10.1007/s10517-020-04822-9DOI Listing

Peculiarities of Collagen Turnover in Aging BALB/c Mice.

Bull Exp Biol Med 2020 May 3;169(1):100-103. Epub 2020 Jun 3.

Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

Examination of collagen turnover in the liver, lungs, and spleen of BALB/c mice revealed the age-related differences in the levels of hydroxyproline and its fractions. The highest level of total hydroxyproline was observed in the lungs and spleen of young (2-month-old) mice. In the liver, this level attained maximum at the age of 6 months at the expense of elevation of protein-bound hydroxyproline relatively its level in 2-month-old mice. Read More

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http://dx.doi.org/10.1007/s10517-020-04833-6DOI Listing

Serum Protein Corona Abolishes Changes in the Expression of Proinflammatory Genes Induced by Quantum Dots in Human Blood Mononuclear Cell.

Bull Exp Biol Med 2020 May 3;169(1):95-99. Epub 2020 Jun 3.

I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.

We studied changes in the transcription of genes encoding cytokines (TNF, IL-6, IL-10, and IL-32), cell activation markers (ICAM1, CD38, Fas, and FCGRIII), ROS production catalyst (NOX2), autophagy (Beclin 1, LC3B, and p62) and apoptosis (BAX, BCL2) regulators in peripheral blood mononuclear cells upon contact with quantum dots CdSe/ZnS-MPA and CdSe/CdSeZnS/ZnS-PTVP. Up-regulation of TNF, ICAM1, Fas, p62 mRNA and down-regulation of the FCGRIII and NOX2 mRNA in response to the presence of quantum dots were revealed. The formation of serum protein corona on the surface of quantum dots abolished this effect. Read More

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http://dx.doi.org/10.1007/s10517-020-04832-7DOI Listing

Comparative Analysis of the Paracrine Action of Neuronal and Glial Progenitor Cells Derived from Induced Human Pluripotent Stem Cells.

Bull Exp Biol Med 2020 May 2;169(1):176-181. Epub 2020 Jun 2.

N. P. Bochkov Research Center for Medical Genetics, Moscow, Russia.

We performed comparative analysis of paracrine activity of neuronal and glial progenitors derived from induced pluripotent stem cells under conditions of hypoxia modeled by addition of cobalt dichloride. Neuronal and glial progenitors produced neuroprotective and neurotrophic effects on SHSY-5Y neuroblastoma cells in co-culture during the post-hypoxic recovery and reduced the number of apoptotic and necrotic cells. Moreover, they produced a neurotrophic effect and promote the formation and growth of neurites in neuroblastoma cells. Read More

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http://dx.doi.org/10.1007/s10517-020-04845-2DOI Listing

Telocytes in the Myocardium of Children with Congenital Heart Disease Tetralogy of Fallot.

Bull Exp Biol Med 2020 May 2;169(1):137-146. Epub 2020 Jun 2.

A. N. Bakulev National Medical Research Center of Cardiovascular Surgery, Ministry of Health of Russian Federation, Moscow, Russia.

Telocytes, a new type of interstitial stem cells with long thin processes that form a three-dimensional network around cardiomyocytes, vessels, and nerve fibers were described in the myocardium of children with tetralogy of Fallot. Two types of morphologically different telocytes, spindle-shaped and rounded, were identified. Contacts of telocytes with stem cells and interstitial macrophages were found. Read More

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http://dx.doi.org/10.1007/s10517-020-04840-7DOI Listing

Evaluation of Stromal Vascular Fraction Properties and Histological Changes of Regenerating Tissue during Healing of Radiation-Induced Lesions in the Rectum.

Bull Exp Biol Med 2020 May 2;169(1):157-161. Epub 2020 Jun 2.

South-Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia.

We analyzed the main properties of autologous adipose-derived stromal vascular fraction (SVF) used for the treatment of radiation-induced lesions in the rectum. No statistically significant correlation between the main characteristics of the cell product (cell number, viability) and patient's age or donor area were revealed. The stages and peculiarities of histological changes in the regenerating tissue after injection of autologous adipose tissue cells were analyzed. Read More

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http://dx.doi.org/10.1007/s10517-020-04842-5DOI Listing

Functional State of Mesenchymal Stem Cells upon Exposure to Bioactive Coatings on Titanium Alloys.

Bull Exp Biol Med 2020 May 2;169(1):147-156. Epub 2020 Jun 2.

Institute of Chemistry, Far-Eastern Division of the Russian Academy of Sciences, Vladivostok, Russia.

Bioactive coatings on implants affect osteogenic differentiation of mesenchymal stem cells (MSC). We studied the morphofunctional state of bone marrow MSC cultured on the surface of calcium phosphate coatings on titanium formed by plasma electrolytic oxidation (PEO). The biocompatible properties of the coatings manifested in the absence of the cytotoxic effect on cells. Read More

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http://dx.doi.org/10.1007/s10517-020-04841-6DOI Listing

Experimental Study of the Possibility of Culturing of Mesenchymal Stromal Cell and Induction of Osteogenic Differentiation on Collagen-Based Scaffolds of Various Modifications.

Bull Exp Biol Med 2020 May 2;169(1):162-168. Epub 2020 Jun 2.

I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.

We analyzed the suitability of various collagen-based scaffolds for culturing and osteogenic differentiation of mesenchymal stromal cells (MSC). Decellularized and lyophilized swine intestinal submucosa (SIS) and porous collagen sponge made from reconstructed bovine derma (PCS) were the most effective in promoting MSC adhesion, survival, and growth. MSC from rat and mouse bone marrow and rat adipose tissue successfully adhered to the scaffold surface and penetrated into its deep layers. Read More

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http://dx.doi.org/10.1007/s10517-020-04843-4DOI Listing

Preservation of Mesenchymal Stem Cell-Derived Extracellular Vesicles after Abdominal Delivery in the Experiment.

Bull Exp Biol Med 2020 May 2;169(1):122-129. Epub 2020 Jun 2.

Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.

Light luminescent microscopy was used to study the distribution of extracellular microvesicles with PKH26-stained membranes secreted by placenta-derived mesenchymal stromal cells in the uterine tissues at different terms after injections to intact rats and after abdominal delivery (a model of cesarian section). Microvesicles migrated through the uterine tissues and were detected for at least 8 days after injection. In some cases, microvesicles were more numerous in the uterus after cesarian section modeling, which can be related to blockade of microcirculation and lymph flow due to inflammation accompanying surgical intervention. Read More

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http://dx.doi.org/10.1007/s10517-020-04838-1DOI Listing

A Group of miRNA as Candidates for Prognostic Biomarkers of Gastric Cancer Metastasis.

Bull Exp Biol Med 2020 May 2;169(1):77-80. Epub 2020 Jun 2.

N. P. Bochkov Research Centre for Medical Genetics, Moscow, Russia.

An association was found between reduced expression of miR-34a, miR-146a with both metastasis to regional lymph nodes (relative risk RR=10.50 and RR=5.25, respectively) and the development of distant metastases (RR=9. Read More

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http://dx.doi.org/10.1007/s10517-020-04828-3DOI Listing

Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.

Bull Exp Biol Med 2020 May 2;169(1):71-76. Epub 2020 Jun 2.

Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH). LEDZ inhalation proved to be more effective in reducing fibrosis severity, both in the lungs and liver. However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver. Read More

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http://dx.doi.org/10.1007/s10517-020-04827-4DOI Listing

Effects of a New Benzimidazole Derivative with Actoprotective Properties on Spermatogenic Epithelium of the Testes and Fertility of Male White Rats.

Bull Exp Biol Med 2020 May 2;169(1):57-59. Epub 2020 Jun 2.

Research Center of Innovative Medicinal Substances, Volgograd State Medical University, the Ministry of Health of the Russian Federation, Volgograd, Russia.

The effects of a new derivative of benzimidazole (K-134) in doses of 5 and 50 mg/kg on the spermatogenesis and fertilizing ability of spermatozoa were studied on male rats. It was found that 2-month course treatment with the studied substance enhances the producing ability of the spermatogenic epithelium and improves fertilizing ability of spermatozoa. Read More

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http://dx.doi.org/10.1007/s10517-020-04823-8DOI Listing

Structural Changes in the Nervous Fibers of the Colon Mucosa in Experimental Acute Colitis.

Bull Exp Biol Med 2020 May 2;169(1):104-109. Epub 2020 Jun 2.

Research Institute of Human Morphology, Moscow, Russia.

Morphological, morphometric, and ultrastructural analysis of the nerve fibers in the colon mucosa was performed in C57BL/6 mice at various terms of development of acute colitis induced by dextran sodium sulphate. The nerve fibers were labeled with antibodies to pan-neuronal marker βIII-tubulin. The progression of inflammatory and ulcerative processes in the mucosa on days 3-5 was associated with hyperplasia and hypertrophy of nerve fibers that peaked on day 7 after colitis induction. Read More

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http://dx.doi.org/10.1007/s10517-020-04834-5DOI Listing

Evaluation of Genotoxicity of Perchlozone, Antituberculous Drug.

Bull Exp Biol Med 2020 May 2;169(1):48-52. Epub 2020 Jun 2.

East Siberian Institute of Medical Ecological Research, Angarsk, Russia.

Experimental studies of Perchlozone, an antituberculous drug with manifest inhibitory activity towards Mycobacterium tuberculosis and Mycobacterium bovis, were carried out. Genotoxicity of Perchlozone was evaluated by the DNA comet method on liver and lung tissues and blood cells after 14-day inhalation exposure of rats. The level of DNA aberrations in response to inhalations of the drug in a concentration of 102. Read More

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http://dx.doi.org/10.1007/s10517-020-04821-wDOI Listing

Dalayed Effects of Antitumor Drug Paclitaxel on the Hereditary Material and Hemopoietic System of CBA Mice.

Bull Exp Biol Med 2020 May 2;169(1):43-47. Epub 2020 Jun 2.

E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

Paclitaxel in a single MTD of 40 mg/kg caused chromosome aberrations and genome changes (polyploidy) in the bone marrow cells of mice early and 3 months after the injection. The quantity of early precursors of erythropoiesis in the bone marrow decreased, as did their proliferative potential irrespective of the animal gender. Injection of paclitaxel in the MTD caused the development of bone marrow hypoplasia during the early period of observation (up to 14 days) and 3 months after injection. Read More

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http://dx.doi.org/10.1007/s10517-020-04820-xDOI Listing

Immunotropic Properties of an Experimental Synthetic Selenium-Organic Compound.

Bull Exp Biol Med 2020 May 2;169(1):40-42. Epub 2020 Jun 2.

Irkutsk Antiplague Research Institute, Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Irkutsk, Russia.

We studied immunotropic properties of synthetic selenium-organic preparation 2,6-dipyridinium-9-selenabicyclo[3.3.1]nonyl dibromide (974zh). Read More

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http://dx.doi.org/10.1007/s10517-020-04819-4DOI Listing

Therapeutic Effects of Salidroside on Cognitive Ability in Rats with Experimental Vascular Dementia.

Bull Exp Biol Med 2020 May 2;169(1):35-39. Epub 2020 Jun 2.

Department of Neurology, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, Zhejiang province, PR China.

We examined the effects of salidroside on cognition in rats with vascular dementia and explored the mechanisms of its neuroprotective effects. Sprague-Dawley rats (n=60) were randomly subdivided into 3 equal groups: controls, untreated rats with vascular dementia, and rats with vascular dementia treated with salidroside (30 mg/kg for 8 weeks). Vascular dementia was provoked by bilateral occlusion of the common carotid arteries. Read More

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http://dx.doi.org/10.1007/s10517-020-04818-5DOI Listing

Cytotoxicity of Potassium Salts of (+)- and (-) Usnic Acid for Paramecium caudatum.

Bull Exp Biol Med 2020 May 2;169(1):110-113. Epub 2020 Jun 2.

Institute for Biological Problems of Cryolithozone, Siberian Division of Russian Academy of Sciences, Yakutsk, Russia.

We analyzed cytotoxicity of water-soluble potassium salts of (+)- and (-) usnic acid (UA) for ciliates P. caudatum. The median lethal concentrations for (+)- and (-) enantiomers did not significantly differ and were 7. Read More

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http://dx.doi.org/10.1007/s10517-020-04835-4DOI Listing

Prostatotropic Action of Glycyrrhizic Acid Disodium Salt in Benign Prostatic Hyperplasia Models.

Bull Exp Biol Med 2020 May 2;169(1):114-118. Epub 2020 Jun 2.

Institute of Molecular Pathology and Pathomorphology, Federal Research Center for Fundamental and Translational Medicine, Novosibirsk, Russia.

The prostatotropic activity of glycyrrhizic acid disodium salt (NaGA) was studied in the models of benign prostatic hyperplasia (BPH) induced by chronic injection of sulpiride (40 mg/kg intraperitoneally for 8 weeks) or testosterone propionate (20 mg/kg subcutaneously for 4 weeks) in the Wistar rats. The oral administration of NaGA in a dose of 100 mg/kg produced a moderate antiproliferative effect in both BPH models resulting in reduced volume density of prostatic epithelium (in the testosterone model) and increased volume density of the glandular lumen (in both models). The observed prostatotropic effects of NaGA were similar to those of Permixon and finasteride, but they were less pronounced as confirmed by smaller drops in epithelial volume density and epithelial-to-stromal ratio compared to the effects of both reference drugs. Read More

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http://dx.doi.org/10.1007/s10517-020-04836-3DOI Listing

Development of Proliferative Response of Thymic Lymphocytes to T-Cell Mitogen in Rats Exposed to Endocrine Disrupter DDT during Ontogeny.

Bull Exp Biol Med 2020 May 2;169(1):60-62. Epub 2020 Jun 2.

Laboratory of Endocrine System Development, Research Institute of Human Morphology, Moscow, Russia.

We studied the formation of proliferative response of thymic lymphocytes to T-cell mitogen in rats exposed to endocrine disrupter DDT during prenatal and postnatal ontogeny. Developmental exposure to the endocrine disruptor was found to attenuate proliferative response during puberty and adulthood due to maintenance of higher proliferation rate of thymic lymphocytes in comparison with age-matched controls. Insufficient proliferative response to mitogens in rats developmentally exposed to the endocrine disrupter increases the risk of impairment of cell-mediated reactions of adaptive immunity. Read More

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http://dx.doi.org/10.1007/s10517-020-04824-7DOI Listing

Dose-Dependent Effect of Bacillus sp. Metabolites from Permafrost on Lymphocyte Differentiation in the Thymus.

Bull Exp Biol Med 2020 May 2;169(1):67-70. Epub 2020 Jun 2.

Federal Research Centre Tyumen Research Centre, Siberian Division of the Russian Academy of Sciences, Tyumen, Russia.

We studied the effect of a single intraperitoneal injection of metabolites from Bacillus sp. M3 strain isolated from permafrost (from 5×10 to 50×10 microbial bodies) on differentiation of T cells in the thymus of F1(CBA/Black-6) mice. On day 21 after the injection of metabolites, a dose-dependent decrease in the level of CD34+CD44+ and an increase in the number of CD34CD44-, CD25-TCR, CD25TCR, CD4CD8-, CD4-CD8, and CD44TCR lymphocytes were observed in the thymus. Read More

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http://dx.doi.org/10.1007/s10517-020-04826-5DOI Listing

A Novel Express Method to Determine Activity of Antitumor Enzyme L-Lysine-α-Oxidase of Trichoderma harzianum Rifai F-180.

Bull Exp Biol Med 2020 May 2;169(1):119-121. Epub 2020 Jun 2.

Russian University of Peoples' Friendship, Moscow, Russia.

A novel express method is developed to determine activity of antitumor enzyme L-lysine-α-oxidase obtained by culturing Trichoderma harzianum Rifai F-180 fungus. The carcinogenic reagent ortho-dianisidine-hydrochloride was replaced in the reaction medium with environmentally friendly reagents of the chromogenic mixture that included tetramethylbenzidine. This method improved precision and sensitivity of ELISA by 10 and 40 times, respectively. Read More

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http://dx.doi.org/10.1007/s10517-020-04837-2DOI Listing

Experimental Study of Heart Functionality during Treatment of Chronic Heart Failure in Rats of Different Age Groups with Levosimendan (Experimental Study).

Bull Exp Biol Med 2020 May 31;169(1):18-23. Epub 2020 May 31.

Department of Anatomy and Physiology of Humans and Animals, Institute of Biology and Chemistry, Moscow State Pedagogical University, Moscow, Russia.

Levosimendan is effective in the therapy of chronic heart failure in both mature and old rats, but the duration of its therapeutic effect differs in these age groups. In 15 days after treatment, the myocardium in old rats maintained augmented functionality, whereas in old animals, functional potencies returned to the levels observed prior to the therapy. Stimulation of tissue respiration with combined administration of L-carnitine and α-lipoic acid against the background of levosimendan treatment prolonged its therapeutic effect in mature, but not in old rats. Read More

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http://dx.doi.org/10.1007/s10517-020-04815-8DOI Listing

Continuous Normobaric Hypoxia Improved Cardiac Bioenergetics after Ischemia/Reperfusion: Role of Opioid Receptors.

Bull Exp Biol Med 2020 May 31;169(1):13-17. Epub 2020 May 31.

Cardiology Research Institute, Tomsk National Research Medical Center, Tomsk, Russia.

We analyzed the role of opioid receptors in the conditioning effect of continuous normobaric hypoxia on bioenergetics of the heart subjected to ischemia/reperfusion injury. Male Wistar rats were adapted to a 21-day continuous normobaric hypoxia (12% pO). Then, the hearts were isolated and subjected to 45-min total ischemia followed by 30-min reperfusion. Read More

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http://dx.doi.org/10.1007/s10517-020-04814-9DOI Listing

Antibodies to Glutamate Facilitate Spatial Memory Formation in the Morris Water Maze in Aging C57BL/6 mice.

Bull Exp Biol Med 2020 May 31;169(1):5-8. Epub 2020 May 31.

School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK.

Intranasal administration of antibodies to glutamate in a dose of 250 μg/kg for two weeks facilitated spatial learning and memory formation in the Morris water maze in aging C57BL/6 mice. In animals treated with glutamate antibodies, the content of serotonin and dopamine metabolites 3-MT and HVA in the hippocampus decreased, but no changes in the metabolism of neurotransmitter acids were revealed. In the prefrontal cortex, dopamine level decreased and the content of its metabolite DOPAC increased; in parallel, an increase in excitatory and inhibitory amino acids (aspartic acid, glutamate, glycine, taurine, and GABA) was observed. Read More

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http://dx.doi.org/10.1007/s10517-020-04812-xDOI Listing

Effects of Anti-Orthostatic Hypokinesia on the Parameters of Vertical Posture: Orthostatic and Stabilometric Study.

Bull Exp Biol Med 2020 May 31;169(1):9-12. Epub 2020 May 31.

Research Institute for Space Medicine, Federal Research Clinical Centre, Federal Medical-Biological Agency of Russia, Moscow, Russia.

The effects of anti-orthostatic hypokinesia on the parameters of vertical posture (orthostatic and postural stability) were studied. Anti-orthostatic hypokinesia was followed by orthostatic instability manifested in a decrease in the tolerance time in the orthostatic test, a significant increase in HR and systolic BP starting from the 11th minute of the test. Significant changes in the parameters reflecting postural stability were observed starting from minutes 10-11 of the stabilometric test. Read More

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http://dx.doi.org/10.1007/s10517-020-04813-wDOI Listing

Ultrastructural Alterations in Granular Neurons of the Dentate Fascia Caused by Intrahippocampal Injection of Beta-Amyloid 1-42.

Bull Exp Biol Med 2020 Apr 23;168(6):802-806. Epub 2020 Apr 23.

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.

The deposition of beta-amyloid (Aβ) in the brain is detected in Alzheimer's disease and during ageing. Until now, ultrastructural studies of changes caused by Aβ in the dentate gyrus are very scarce. The effects of Aβ 1-42 injection into the CA1 field of rat hippocampus were studied by electron microscopy. Read More

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http://dx.doi.org/10.1007/s10517-020-04806-9DOI Listing

The Role of Cardiac Opioid Receptors in the Cardioprotective Effect of Continuous Normobaric Hypoxia.

Bull Exp Biol Med 2020 Apr 27;168(6):727-729. Epub 2020 Apr 27.

Cardiology Research Institute, Tomsk National Research Medical Center, Tomsk, Russia.

We studied the role of opioid receptor subtypes in improvement of the functional state of the heart during reperfusion after adaptation to continuous normobaric hypoxia. To this end, male Wistar rats were subjected to continuous normobaric hypoxia (12% O). Then, the hearts were isolated and exposed to total 45-min ischemia followed by 30-min reperfusion. Read More

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http://dx.doi.org/10.1007/s10517-020-04789-7DOI Listing

Metabolism of a New Antiaggregant, Indolinone Derivative.

Bull Exp Biol Med 2020 Apr 25;168(6):739-742. Epub 2020 Apr 25.

E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

Cytochrome p450-mediated metabolism of GRS (indolinone antiaggregant) and its effects on activities of cytochrome p450 isoenzymes were studied. Inhibition of 6 isomers of cytochrome p450 in human liver microsomes was studied with the use of specific substrates. It was found that human liver cytochrome p450 enzymes could not induce degradation of GRS and that GRS was not an inductor or inhibitor of cytochrome p450 family members 1A2, 2C9, 2C19, 2D6, 2C8, and 3A4. Read More

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http://dx.doi.org/10.1007/s10517-020-04792-yDOI Listing

Specific Features of Interactions between Megakaryocytic and Granulocytic Hematopoiesis Lineages and Myelofibrosis during the Acute Phase of Chronic Myeloid Leukemia, Chronic Lymphocytic Leukemia, and Multiple Myeloma.

Bull Exp Biol Med 2020 Apr 25;168(6):734-738. Epub 2020 Apr 25.

Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

The specific features of interactions between megakaryocytic and granulocytic hematopoiesis lineages and myelofibrosis were studied in patients with active phase (before treatment) of chronic myeloid leukemia, chronic lymphocytic leukemia, and multiple myeloma. In patients with chronic myeloid leukemia, a direct correlation was found between the severity of both early and advanced myelofibrosis and the number of megakaryocytes in the bone marrow irrespectively of the type of the bone marrow tumor. The parameters of granulocytic hematopoiesis lineage were higher in myelofibrosis. Read More

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http://dx.doi.org/10.1007/s10517-020-04791-zDOI Listing

Experimental Study of the Biodistribution of New Bone-Seeking Compounds Based on Phosphonic Acids and Gallium-68.

Bull Exp Biol Med 2020 Apr 25;168(6):777-780. Epub 2020 Apr 25.

National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russia.

We investigate biodistribution of gallium-labeled hydroxyethylidenediphosphonic acid (Ga-HEDP) and diethylenetriaminepentakis(methylenephosphonic acid) (Ga-DTPMP) in intact Wistar rats. It was shown that Ga-DTPMP accumulated mainly in the bone tissue providing high femur/blood and femur/muscle ratios and had high stability in vivo. In contrast, Ga-HEDP was characterized by low stability and high uptake of radioactivity in blood throughout the study. Read More

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http://dx.doi.org/10.1007/s10517-020-04800-1DOI Listing

Social and Cognitive Impairments in Rat Offspring after Ultrasound-Induced Prenatal Stress.

Bull Exp Biol Med 2020 Apr 25;168(6):730-733. Epub 2020 Apr 25.

Department of the Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.

We studied the possibility of developing an autism model based on chronic prenatal psychological stress caused by variable frequency ultrasound 20-45 kHz. The offspring of female rats stressed during pregnancy demonstrated reduced time of social contacts in the social interaction test, increased anxiety in the open-field test, and memory impairment in the Morris water maze test in comparison with the control (intact) rat offspring. We also found a reducing trend in the BDNF gene expression in the amygdala in males of the experimental group. Read More

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http://dx.doi.org/10.1007/s10517-020-04790-0DOI Listing

Expression of Metalloproteinases and Type I and III Collagens during Healing of Excisional Skin Wound on the Abdomen and Back in Rats.

Bull Exp Biol Med 2020 Apr 23;168(6):812-816. Epub 2020 Apr 23.

Research Institute of Human Morphology, Moscow, Russia.

The study was carried out using a novel rat model developed in our laboratory, namely16 mm diameter circular excisional wounds were generated on the abdomen which resulted in minimal scarring. Restoration of the skin integrity was completed by day 60 after the wounding surgery. By this time, regenerates on the abdomen were stronger than on the back (at, respectively, 58 and 17. Read More

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http://dx.doi.org/10.1007/s10517-020-04808-7DOI Listing

Immunohistochemical Study of Dark and Progenitor Carotid Body Cells: Artefacts or Real Subtypes?

Bull Exp Biol Med 2020 Apr 23;168(6):807-811. Epub 2020 Apr 23.

Research Institute of Human Morphology, Moscow, Russia.

Postmortem changes occurring in human carotid body were simulated on the Wistar rat model. It was shown that light, dark, and pyknotic (progenitor) subtypes of human carotid body cells are an artifact and cannot be used in clinical practice to study the characteristics of various human diseases. The differences between the control group of healthy individuals and individuals with the various pathologies are most likely due to the different levels of premortal hypoxia that the tissue had been exposed to. Read More

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http://dx.doi.org/10.1007/s10517-020-04807-8DOI Listing

Method of Surgical Treatment of Spine Injury with the Use of Extracorporeal Navigation System Based on Additive Technologies.

Bull Exp Biol Med 2020 Apr 23;168(6):821-825. Epub 2020 Apr 23.

Ural Research Institute of Phthisiopulmonology - Affiliated Branch of the National Medical Research Center for Phthisiopulmonology and Infectious Diseases, the Ministry of Health of Russia, Yekaterinburg, Russia.

We present a method of minimally invasive transcutaneous insertion of screws using a prefabricated extracorporeal navigation system using additive technologies (based on primary data obtained from the DICOM package in multi-detector computed tomography of the affected spine segment) according to the principle of personalized medicine. The method was tested on 10 dogs of different breeds with generally similar mechanism of trauma and typical consequences that led to fracture and dislocation of one of the lumbar vertebrae. In all animals, a positive treatment outcome of different degrees was achieved. Read More

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http://dx.doi.org/10.1007/s10517-020-04810-zDOI Listing

Blockade of Dopamine D2 Receptors as a Novel Approach to Stimulation of Notch1 Endothelial Progenitor Cells and Angiogenesis in C57BL/6 Mice with Pulmonary Emphysema Induced by Proteases and Deficiency of α1-Antitrypsin.

Bull Exp Biol Med 2020 Apr 23;168(6):718-723. Epub 2020 Apr 23.

Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Moscow, Russia.

We studied the effects of spiperone, a selective blocker of dopamine D2 receptors, on the model of pulmonary emphysema provoked by administration of elastase and D-galactosamine hydrochloride to female C57BL/6 mice and characterized by activation of proteases in the lungs and systemic deficiency of its inhibitor α1-antitrypsin. In this model, spiperone prevented the development of inflammatory reaction and reduced the area of emphysematous expanded alveolar tissue. The expression of angiogenic marker CD31 in the lungs increased under these conditions. Read More

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http://dx.doi.org/10.1007/s10517-020-04787-9DOI Listing

Changes in Transcriptional Regulation of Postnatal Morphogenesis of the Adrenal Zona Fasciculata Caused by Endocrine Disruptor Dichlorodiphenyltrichloroethane.

Bull Exp Biol Med 2020 Apr 23;168(6):797-801. Epub 2020 Apr 23.

Laboratory of Biochemistry of Nitrogen Fixation and Nitrogen Metabolism, A. N. Bach Institute of Biochemistry, Federal Research Center of Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia.

We studied the expression of transcriptional factors regulating postnatal morphogenesis of the adrenal zona fasciculata in rats after developmental exposure to endocrine disruptor DDT. It was found that tissue reparation after trophic disorders and cell death triggered by prenatal and postnatal exposure to DDT was accompanied by an increase in the number of Oct4- and Shh-expressing cells forming a pool located outside the regeneration zones and involved in the maintenance of tissue homeostasis in the zona fasciculata. DDT exposure also disrupted the expression of antiproliferative factor Hhex. Read More

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http://dx.doi.org/10.1007/s10517-020-04805-wDOI Listing

Effect of Platinum Nanoparticles on the Structure of Protein Molecules and Regulation of the Tone of Brain Vessels in Experimental Animals.

Bull Exp Biol Med 2020 Apr 23;168(6):781-784. Epub 2020 Apr 23.

Research Institute of General Pathology and Pathophysiology, Moscow, Russia.

The effects of platinum nanoparticles on the morphological structures of the solid phase of blood serum and the tone of cerebral microvessels as indicators of the dynamics of homeostasis were studied on outbred albino male rats (n=40) weighing 300-350 g. Platinum nanoparticles were injected to experimental animals in 1 ml of physiological saline. For systemic BP measurements and blood sampling, the femoral artery was isolated and catheterized. Read More

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http://dx.doi.org/10.1007/s10517-020-04801-0DOI Listing

Distribution of Ferrihydrite Nanoparticles in the Body and Possibility of Controlling Them in an Isolated Organ by a Permanent Magnetic Field.

Bull Exp Biol Med 2020 Apr 23;168(6):789-792. Epub 2020 Apr 23.

Federal Research Center Krasnoyarsk Research Center, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, Russia.

We studied the distribution of ferrihydrite nanoparticles isolated from bacteria Klebsiella oxytoca in the whole body in vivo and in a cultured isolated organ (liver). The possibility of controlling these nanoparticles in the body using a magnetic field was assessed. One hour after intravenous injection of ferrihydrite nanoparticles to mice, their accumulation was observed in the liver, lungs, and kidneys. Read More

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http://dx.doi.org/10.1007/s10517-020-04803-yDOI Listing

Parameters of Experimental Activity in the Study of the Cognitive Abilities of Rhesus Monkeys (Macaca mulatta) and Baboons (Papio hamadryas).

Bull Exp Biol Med 2020 Apr 23;168(6):793-796. Epub 2020 Apr 23.

Research Institute of Medical Primatology, Sochi, Russia.

We compared experimental activity, behavioral activity in the experiment, of rhesus monkeys (Macaca mulatta) and baboons (Papio hamadryas). Baboons showed higher levels of experimental activity. The contrast in the activity between the species is probably associated with species-specific characteristics of the behavior and different ability to adapt under new conditions. Read More

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http://dx.doi.org/10.1007/s10517-020-04804-xDOI Listing

Rat Blood Leukocytes after Intravenous Injection of Chitosan-Modified Magnetic Nanospheres.

Bull Exp Biol Med 2020 Apr 23;168(6):785-788. Epub 2020 Apr 23.

Department of Morphology and Pathology, Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

Nanosized magnetite particles (magnetic nanospheres) are a prospective basis for creation of new diagnostic and therapeutic agents. The structure of blood leukocytes and the leukocytic formula are studied in adult rats over a period of 120 days after a single intravenous injection of chitosan-modified nanosized magnetite particles. No effects of chitosan-modified magnetic nanospheres on the structure of rat blood leukocytes are detected. Read More

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http://dx.doi.org/10.1007/s10517-020-04802-zDOI Listing