7,552 results match your criteria Bulletin Of Experimental Biology And Medicine[Journal]


The Effect of Electromagnetic Fields of Extremely Low Frequency 30 Hz on Rat Ovaries.

Bull Exp Biol Med 2019 Mar 23;166(5):704-707. Epub 2019 Mar 23.

State Scientific Research Test Institute of Military Medicine, St. Petersburg, Russia.

We studied the effects of extremely low-frequency electromagnetic field on the ovaries in rats. The female rats were exposed to a 30 Hz sinusoidal magnetic field of 4 kA/m for 2 h per day for a period of 10 weeks, including the period of embryonic development. After the exposure, the duration of the estrous cycle and serum levels of luteinizing hormone, follicle stimulating hormone, progesterone, and estradiol E2 were measured in different phases of the estrous cycle and morphological changes in the ovaries were evaluated. Read More

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http://link.springer.com/10.1007/s10517-019-04422-2
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http://dx.doi.org/10.1007/s10517-019-04422-2DOI Listing
March 2019
2 Reads

Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats.

Bull Exp Biol Med 2019 Mar 23;166(5):699-703. Epub 2019 Mar 23.

Research Institute of Human Morphology, Moscow, Russia.

Hypoxia tolerance and the intensity of systemic inflammatory response during endotoxemia were examined in newborn, prepubertal, and mature Wistar rats. To assess the sensitivity to hypoxia, the rats were placed into a pressure chamber simulating an altitude of 11,500 m. The systemic inflammatory response was provoked by intraperitoneal injection of LPS from E. Read More

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http://dx.doi.org/10.1007/s10517-019-04421-3DOI Listing

Increased Blood Concentration of IgG Degradation Products in Prostate Cancer.

Bull Exp Biol Med 2019 Mar 23;166(5):651-655. Epub 2019 Mar 23.

N. M. Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.

For elucidation of the mechanisms of IgG binding with human plasminogen in prostate cancer patients, we propose an original ELISA on polystyrene plates with immobilized heavy and light plasminogen chains. The level of IgG bound to plasminogen heavy chain in the serum of prostate cancer patients significantly exceeded that in healthy volunteers. IgG treated with plasmin more actively (by more than 2 times) bound plasminogen heavy chain than intact IgG. Read More

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http://dx.doi.org/10.1007/s10517-019-04411-5DOI Listing

Cellular Composition and Expression of Cell Proliferation and Apoptosis Markers in Bone Marrow Cells after Heterotransplantation.

Bull Exp Biol Med 2019 Mar 23;166(5):695-698. Epub 2019 Mar 23.

Department of General and Clinical Morphology and Forensic Medicine, I. N. Ulyanov Chuvash State University, Cheboksary, Chuvash Republic, Russia.

The effects of heterotransplantation on bone marrow structures were studied in experimental male mice. Suspension of the bone marrow from feline femur (1 ml bone marrow diluted in isotonic saline, 1:2) was injected into the caudal vein. Heterotransplantation led to an increase in the level of serotonin involved in cell differentiation. Read More

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http://dx.doi.org/10.1007/s10517-019-04420-4DOI Listing

Abnormal Membrane Localization of α2 Isoform of Na,K-ATPase in m. soleus of Dysferlin-Deficient Mice.

Bull Exp Biol Med 2019 Mar 23;166(5):593-597. Epub 2019 Mar 23.

Department of General Physiology, St. Petersburg State University, St. Petersburg, Russia.

Dysferlin protein plays a key role in the multimolecular complex responsible for the maintenance of sarcolemma integrity and skeletal muscle cell functioning. We studied the membrane distribution of nicotinic acetylcholine receptors and α2 isoform of Na,K-ATPase in motor endplates of m. soleus in dysferlin-deficient Bla/J mice (a dysferlinopathy model). Read More

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http://dx.doi.org/10.1007/s10517-019-04398-zDOI Listing

The Role of NO Synthase in the Cardioprotective Effect of Substances of Humic Origin on the Model of Ischemia and Reperfusion of Isolated Rat Heart.

Bull Exp Biol Med 2019 Mar 23;166(5):598-601. Epub 2019 Mar 23.

E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

The cardioprotective and inotropic effects of standardized active natural substance based on high-molecular-weight compounds of humic origin were studied on the model of global ischemia (40 min) and reperfusion of isolated perfused rat heart. Preventive administration of the test substance (0.1 mg/ml) before ischemia/reperfusion modeling reduced reperfusion contracture and necrotic death of cardiomyocytes and promoted recovery of myocardial contractility. Read More

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http://dx.doi.org/10.1007/s10517-019-04399-yDOI Listing

Study of the Biocompatibility of Titanium-Niobium Implants by the Parameters of Their Osseointegration under Experimental Conditions.

Bull Exp Biol Med 2019 Mar 22;166(5):686-688. Epub 2019 Mar 22.

Clinical Center for Dentistry, Federal Medical-Biological Agency of the Russian Federation, Moscow, Russia.

Superelastic titanium alloys (titanium-niobium-zirconium and titanium-niobium-tantalum) are considered to be the more advantageous choice for dental implants than titanium alloys due to their physico-mechanical properties close to those of the bone tissue. Morphological studies of the biocompatibility of these alloys in comparison with titanium were carried out in vivo. Scanning electron microscopy and microprobe elemental analysis of the tissue along the interface between the bone and titanium/superelastic titanium alloys showed similar morphology and elemental composition both on days 30 (poorly mineralized connective tissue was detected in sites of contact with titanium alloy samples) and 90 after surgery (the surface of the titanium alloy samples was covered with mineralized tissue similar by its composition to the surrounding bone). Read More

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http://dx.doi.org/10.1007/s10517-019-04418-yDOI Listing

Death Mechanisms of Pulmonary Alveolocytes in Mice Infected with Influenza Viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05.

Bull Exp Biol Med 2019 Mar 22;166(5):637-640. Epub 2019 Mar 22.

Optimum Medical Laboratory, Research-and-Educational Center, Sochi, Russia.

In CBA mice infected with influenza viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05 in a dose of 10 MLD, the mechanisms of death of pulmonary alveolocytes over 10 postinfection days were studied by light microscopy, immunohistochemistry, and morphometry. In mice infected with A/H1N1, alveolocytes died predominantly via necrosis, while apoptosis mostly employed the mitochondrial pathway. In mice infected with A/H5N1, apoptosis was the dominant mechanism of alveolocyte death proceeded via membrane receptor signaling followed by switching to FAS-mediated pathway via activation of FADD, the apoptotic signal transduction protein. Read More

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http://link.springer.com/10.1007/s10517-019-04408-0
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http://dx.doi.org/10.1007/s10517-019-04408-0DOI Listing
March 2019
2 Reads

Induced Pluripotent Stem Cell-Derived Hematopoietic Embryoid Bodies Improve Mouse Status in Septic Peritonitis.

Bull Exp Biol Med 2019 Mar 22;166(5):667-670. Epub 2019 Mar 22.

Department of Legal Medicine, Nara Medical University, Kashihara, Nara, Japan.

We examined the efficacy of embryoid bodies from 6-day induced pluripotent stem cells an in vivo sepsis model. Injection of embryoid bodies to septic mice improved the condition of their lungs and significantly increased their survival rate. Although embryoid bodies secretedsphingosine-1-phosphate in vitro, its serum levels in mouse plasma were significantly reduced compared to that in the control (untreated mice receiving PBS). Read More

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http://dx.doi.org/10.1007/s10517-019-04414-2DOI Listing
March 2019
1 Read

Structure of Rat Testicles after Intravenous Injection of Nanosized Magnetite Particles.

Bull Exp Biol Med 2019 Mar 22;166(5):680-685. Epub 2019 Mar 22.

Department of X-Ray and Tomographic Diagnostic Methods, Research Institute of Cardiology, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

The structure of the testicles was studied in adult rats in 120 days after a single intravenous injection of chitosan-modified (magnetic nanospheres) and lipid-modified (magnetoliposomes) nanosized magnetite particles. Perls histochemical reaction detected in the testicular interstitial connective tissue the cells which absorbed and accumulated magnetite nanoparticles. The dynamics of spermatogenesis index and the count of Perls cells in the rat testicles were traced throughout the experiment. Read More

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http://link.springer.com/10.1007/s10517-019-04417-z
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http://dx.doi.org/10.1007/s10517-019-04417-zDOI Listing
March 2019
4 Reads

Possibilities of Postmortem Magnetic Resonance Imaging for Evaluation of Anasarca in Newborns.

Bull Exp Biol Med 2019 Mar 22;166(5):671-675. Epub 2019 Mar 22.

V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.

We studied the possibilities of postmortem magnetic resonance imaging (MRI) for evaluation of anasarca in newborns. Before the autopsy, MRI in T1 and T2 standard modes was performed, signal intensity tomograms in the subcutaneous tissue were analyzed, and tissue hydration index was calculated. Using 3D reconstruction, we determined the values of hydrothorax, hydropericardium, and ascites, as well as specific volumes of fluid in the abdominal and thoracic cavities. Read More

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http://dx.doi.org/10.1007/s10517-019-04415-1DOI Listing

Cytokine Profiling of Subclinical Tick-Borne Infections in Humans.

Bull Exp Biol Med 2019 Mar 22;166(5):622-625. Epub 2019 Mar 22.

Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.

Over many years, tick-borne infections remain one of the most serious threats to human health worldwide. The immune response to these infections in a human after confirmed bite by an infected carrier at the early stages of infection in the absence of clinical symptoms can be the first indicator of the presence of the infectious agent in the body. During viral infection, the concentration of IL-1α, IL-8, IL-10, IL-17A, and IFNγ increases; superoxide dismutase also increases, in contrast to bacterial infections. Read More

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http://dx.doi.org/10.1007/s10517-019-04405-3DOI Listing

Contribution of Fetal Programming in the Formation of Cognitive Impairments Induced by Lead Poisoning in White Rats.

Bull Exp Biol Med 2019 Mar 22;166(5):617-621. Epub 2019 Mar 22.

East Siberian Institute of Medical and Ecological Research, Angarsk, Russia.

The contribution of prenatal hypoxic damage to the CNS to the formation of high sensitivity of the body to lead acetate was studied. Prenatal fetal hypoxia was modeled by the administration of sodium nitrite in doses of 5, 25, and 50 mg/kg to pregnant female rats. Cognitive capacities of mature offspring were evaluated in the radial maze test and Morris water maze test. Read More

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http://dx.doi.org/10.1007/s10517-019-04404-4DOI Listing

Synthetic Phenolic Antioxidant TS-13 Suppresses the Growth of Lewis Lung Carcinoma and Potentiates Oncolytic Effect of Doxorubicin.

Bull Exp Biol Med 2019 Mar 22;166(5):646-650. Epub 2019 Mar 22.

Belarusian State University, Minsk, Republic of Belarus.

ROS are important intracellular messengers; their ambiguous role in malignant processes was demonstrated in many studies. The effects of a synthetic phenolic antioxidant sodium 3-(3'-tert-butyl-4'-hydroxyphenyl)propyl thiosulfonate sodium (TS-13) on the tumor growth and oncolytic properties of doxorubicin were studied in the experimental model of Lewis lung carcinoma in mice. In mice receiving TS-13 with drinking water (100 mg/kg), suppression of tumor growth by 32. Read More

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http://link.springer.com/10.1007/s10517-019-04410-6
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http://dx.doi.org/10.1007/s10517-019-04410-6DOI Listing
March 2019
6 Reads

Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells from the Apical Side of the Membrane under Static and Microcirculation Conditions.

Bull Exp Biol Med 2019 Mar 22;166(5):626-630. Epub 2019 Mar 22.

BioClinicum Research Center, Moscow, Russia.

Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulating natural environment of the body in comparison with static culturing. Impedance spectroscopy (BioClinicum Research Center) was used for non-invasive estimation of the monolayer quality and changes in the cell apical membrane due to the formation of microvilli. Read More

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http://dx.doi.org/10.1007/s10517-019-04406-2DOI Listing
March 2019
1 Read

Amino Acid Substitutions N123D and N149D in Hemagglutinin Molecule Enhance Immunigenicity of Live Attenuated Influenza H7N9 Vaccine Strain in Experiment.

Bull Exp Biol Med 2019 Mar 22;166(5):631-636. Epub 2019 Mar 22.

A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine, St. Petersburg, Russia.

We compared three cold-adapted live attenuated influenza vaccine strains prepared by reverse genetics methods on the basis of master donor virus A/Leningrad/134/17/57 and influenza H7N9 strains A/Anhui/1/2013 and A/Shanghai/1/2013. Two strains based on A/Anhui/1/2013 differed by amino acid positions 123 and 149 in HA1 (123N/149N; 123D/149D). All strains efficiently replicated in developing chicken embryos; A/Shanghai/1/2013-based strain and A/Anhui/1/2013-123N/149N variant were characterized by reduced replication in MDCK cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04407-1DOI Listing

Behavioral and Cognitive Reactions of Rats with Experimental Chronic Obstructive Lung Diseases of Different Degree.

Bull Exp Biol Med 2019 Mar 22;166(5):602-605. Epub 2019 Mar 22.

School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia.

The study presents the results of behavioral phenotyping of rats with experimental chronic obstructive pulmonary disease (eCOPD) of different severity modeled by using a combination of inductors (purified papain and bacterial LPS) in increasing concentrations. Locomotor and exploratory activity, spatial memory, and anxiety were evaluated. In mild eCOPD, locomotor and exploratory activity increased, while other parameters did not differ from the control values. Read More

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http://link.springer.com/10.1007/s10517-019-04400-8
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http://dx.doi.org/10.1007/s10517-019-04400-8DOI Listing
March 2019
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Effect of 4-Methyl-Phenyl Biguanide on Free Radical Homeostasis and Activity of Oxidative Metabolism Enzymes in Rats with Cardiovascular Pathology Developing against the Background of Rheumatoid Arthritis.

Bull Exp Biol Med 2019 Mar 22;166(5):606-609. Epub 2019 Mar 22.

Department of Medical Biochemistry and Microbiology, Voronezh State University, Voronezh, Russia.

We studied the effect of 4-methyl-phenyl biguanide, a substance chosen by PASS software (Prediction of Activity Spectra for Substance), on oxidative status and activity of oxidation metabolism enzymes participating in limitation free radical processes (aconitate hydratase, glucose-6-phosphate dehydrogenase, and NADP-isocitrate dehydrogenase) in the heart and blood serum of rats with cardiovascular pathology developing against the background of experimental rheumatoid arthritis. Activity of cardiomyocyte cytolysis markers in rat blood serum was also measured. Normalization of the studied parameters under the influence of the test compound in animals with experimental pathology attests to its positive effects on metabolic and free radical processes. Read More

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http://dx.doi.org/10.1007/s10517-019-04401-7DOI Listing
March 2019
2 Reads

Structural Analysis of the Myocardium in Experimental Anthracycline-Induced Cardiomyopathy Combined with Adrenergic Stimulation.

Bull Exp Biol Med 2019 Mar 22;166(5):689-694. Epub 2019 Mar 22.

Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

We studied dynamic changes in the total number of cardiomyocytes and the character of structural lesions in the myocardium in rats with modeled anthracycline-induced cardiomyopathy provoked by a single injection of doxorubicin in a dose of 10 mg/kg alone or in combination with subsequent adrenergic stimulation. The injections of epinephrine during the development of anthracycline-induced cardiomyopathy resulted in more pronounced loss of body weight, stronger decrement of the heart weight, and more severe decrease of the cardiomyocyte count in comparison with the corresponding changes induced by doxorubicin alone. The basic lesions of cardiomyocytes in anthracycline-induced cardiomyopathy are the lytic alterations and subsegmental contractures; in contrast, combined use of doxorubicin and epinephrine provoked degree II and III contractures. Read More

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http://dx.doi.org/10.1007/s10517-019-04419-xDOI Listing

Transcriptome Guided Drug Combination Suppresses Proliferation of Breast Cancer Cells.

Bull Exp Biol Med 2019 Mar 22;166(5):656-660. Epub 2019 Mar 22.

P. A. Hertsen Moscow Oncology Research Center, Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.

One of actively developing trends in modern pharmacology is the use of the transcriptome analysis for drug repositioning. We have previously detected two molecular markers of relapses in patients with malignant breast tumors: ELOVL5 and IGFBP6. Poor prognosis is associated with low expression of these markers. Read More

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http://dx.doi.org/10.1007/s10517-019-04412-4DOI Listing

Comparison of Reactivity of the Lipid Peroxidation-Antioxidant Defense System in Normal and Hypertensive Rats at Different Stages of Stress-Reaction.

Bull Exp Biol Med 2019 Mar 22;166(5):613-616. Epub 2019 Mar 22.

Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.

We compared changes in the parameters of LPO-antioxidant defense system at various stages of the stress response in mature male ISIAH rats (hereditary stress-induced arterial hypertension) and WAG rats in 3 h (stage of anxiety) and 7 days (resistance stage) after a 3-h single immobilization in the supine position. It was found that post-stress reactivity of the nonspecific stress-limiting LPO system is different in normotensive and hypertensive rats. ISIAH rats, irrespective of the stage of the stress-reaction, demonstrated more intense LPO reactions (accumulation of TBA-reactive products) and reduced antioxidant defense parameters (low content of lipid-soluble vitamins, increased oxidized glutathione content) in comparison with the corresponding parameters in WAG rats. Read More

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http://dx.doi.org/10.1007/s10517-019-04403-5DOI Listing
March 2019
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Expression of Aβ42, τ-Protein, p16, p53 in Buccal Epithelium: Prospects for Use in the Diagnostics of Alzheimer's Disease and Rate of Aging.

Bull Exp Biol Med 2019 Mar 22;166(5):676-679. Epub 2019 Mar 22.

St. Petersburg Research Center Institute of Bioregulation and Gerontology, St. Petersburg, Russia.

The expression of Aβ42, and τ-protein, and p16 and p53 proteins was analyzed in the buccal epithelium of elderly and senile patients with Alzheimer's disease. We revealed enhanced synthesis of Alzheimer's disease markers Aβ42 (by 15-30 times) and τ-protein (by 5 times) in comparison with the corresponding values in people without neurodegenerative pathology of the same age groups. In addition, increased synthesis of proteins of cell aging and apoptosis p16 (by 6-10 times) and p53 (by 2-3 times) was observed in patients in comparison with age-matched persons without neuropathology. Read More

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http://dx.doi.org/10.1007/s10517-019-04416-0DOI Listing

Evaluation of Lipid Peroxidation-Antioxidant Defense System Depending on the Stage of Stress Reaction in Hypertensive ISIAH Rats.

Bull Exp Biol Med 2019 Mar 22;166(5):610-612. Epub 2019 Mar 22.

Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.

The stages of stress reaction (anxiety and resistance) were reproduced on the model of stresssensitive hypertensive ISIAH rats. Analysis of the experimental data showed an increase in activity of the antioxidant defense system in ISIAH rats: total antioxidant activity of the blood and superoxide dismutase activity increased at the stage of anxiety and then decreased during the resistance stage. Read More

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http://dx.doi.org/10.1007/s10517-019-04402-6DOI Listing

Changes in the Metastatic Properties of MDA-MB-231 Cells after IGFBP6 Gene Knockdown Is Associated with Increased Expression of miRNA Genes Controlling INSR, IGF1R, and CCND1 Genes.

Bull Exp Biol Med 2019 Mar 22;166(5):641-645. Epub 2019 Mar 22.

A. F. Tsyb Medical Radiological Research Center, National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Obninsk, Moscow Region, Russia.

Metastatic cascade is associated with the process of epithelial-mesenchymal transition accompanied by changes in cell proliferation, migration, adhesion, and invasiveness mediated by the insulin-like growth factor (IGF) signal pathway. IGFBP6 protein binds IGF and prevents its interaction with receptors. IGFBP6 gene knockdown through RNA-interference inhibits cell migration and increased the rate of proliferation of breast cancer MDA-MB-231 cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04409-zDOI Listing

Antimetastatic Activity of Combined Topotecan and Tyrosyl-DNA Phosphodiesterase-1 Inhibitor on Modeled Lewis Lung Carcinoma.

Bull Exp Biol Med 2019 Mar 22;166(5):661-666. Epub 2019 Mar 22.

Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.

The antimetastatic activity of combined or individual administration of topotecan and tyrosyl-DNA phosphodiesterase 1 (Tdp1) inhibitor was examined under various administration schedules in mice with Lewis lung carcinoma modeled by intravenous injection of 200,000 clone/mouse. The greatest antimetastatic effect was observed after combined use of topotecan and Tdp1 inhibitor as documented by macroscopic study of the lungs that revealed the decreased metastatic scores by 76, 91, or 74% at the respective inhibitor doses of 2, 4, or 6 mg/mouse, respectively, in parallel with inhibition of metastasis up to 98% (at inhibitor dose of 4 mg/mouse) and morphological and morphometric analyses of the lung sections, which revealed elevation of metastasis growth delay index to 86 and 63% at the respective inhibitor doses of 4 and 6 mg/mouse, respectively. The combined administration of topotecan and Tdp1 inhibitor is viewed as the most effective way to eliminate the metastatic formations with possible restitution of focal lesions. Read More

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http://dx.doi.org/10.1007/s10517-019-04413-3DOI Listing

Correction to: Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of the Pegylated Form of Glucagon-Like Peptide 1.

Bull Exp Biol Med 2019 02;166(4):591

Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

The title of the article should read:"Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of the Pegylated Form of Glucagon-Like Peptide 1". Read More

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http://dx.doi.org/10.1007/s10517-019-04397-0DOI Listing
February 2019

Comparative Analysis of Bioactivity of the Russian-Made Antitumor Substances of the Nitrosourea Group.

Bull Exp Biol Med 2019 Feb 27;166(4):456-460. Epub 2019 Feb 27.

I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation, Moscow, Russia.

We performed an in vivo comparative study of activity of three substances of the nitrosourea group produced in Russia. All substances demonstrated high antitumor activity against various solid and leukemic tumors. Aranosa significantly enhanced life duration in mice with leukemia (by 65-194%) and inhibited the growth of solid tumors (by 49-99. Read More

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http://dx.doi.org/10.1007/s10517-019-04371-wDOI Listing
February 2019
2 Reads

Application of Adeno-Associated Virus Vectors for Engineering SCF-Containing Extracellular Vesicles of Mesenchymal Stromal Cells.

Bull Exp Biol Med 2019 Feb 22;166(4):527-534. Epub 2019 Feb 22.

National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia.

Mesenchymal stromal cells from rat adipose tissue were transduced with adeno-associated viral (AAV) vector encoding stem cell factor SCF that stimulates proliferation of cardiac c-kit cells and improved cardiac function and survival of animals after myocardial infarction. Extracellular vesicles isolated from the medium conditioned by mesenchymal stromal cells by ultracentrifugation were characterized by Western blotting, transmission electron microscopy, nanoparticle tracking analysis, immunostaining, and mass spectrometry analysis. Using proteomic analysis, we identified transgenic SCF in extracellular vesicles released by AAV-modified mesenchymal stromal cells and detected some proteins specific of extracellular vesicles secreted by transduced cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04387-2DOI Listing
February 2019

Comparative Analysis of Secretome of Human Umbilical Cord- and Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells.

Bull Exp Biol Med 2019 Feb 22;166(4):535-540. Epub 2019 Feb 22.

V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.

Production of cytokines and growth factors by cultured human umbilical cord tissue- and bone marrow-derived multipotent mesenchymal stromal cells was measured by multiplex analysis. In most cases, the concentrations of bioactive factors in the culture medium conditioned by umbilical cord-derived cells was ten- to hundred-times higher than in the medium conditioned by bone marrow-derived cells. These results suggest that both multipotent mesenchymal stromal cells from the umbilical cord and cell-free products can have more pronounced therapeutic effect in comparison with mesenchymal stromal cells obtained from "adult" sources. Read More

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http://dx.doi.org/10.1007/s10517-019-04388-1DOI Listing
February 2019

Effect of Transcutaneous Electrical Stimulation of Nerves on Blood Pressure and Blood Content of Neuropeptide CGRP and Nitric Oxide in Hypertensive Rats with Metabolic Disturbances.

Bull Exp Biol Med 2019 Feb 21;166(4):436-439. Epub 2019 Feb 21.

Research Institute of Physiology and Fundamental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.

The development of arterial hypertension in male Wistar rats with fructose-induced metabolic syndrome (12.5% of fructose solution as the only drinking source for 10 weeks) along with impaired glucose tolerance and increased serum concentration of triglycerides and LPO products caused a decrease in the content of serum blood calcitonin gene-related peptide (CGRP). Low-frequency transcutaneous electrical nerve stimulation (1 mA, 2 Hz, 10 min daily for 2 weeks) performed in 8 weeks after the beginning of fructose treatment reduced systolic BP and serum concentration of triglycerides and LPO produces and improved glucose tolerance. Read More

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http://dx.doi.org/10.1007/s10517-019-04367-6DOI Listing
February 2019

Cytotoxic Activity of Peripheral Blood NK Cells towards Trophoblast Cells during Pregnancy.

Bull Exp Biol Med 2019 Feb 20;166(4):567-573. Epub 2019 Feb 20.

D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia.

We evaluated cytotoxic activity of peripheral blood NK cells towards trophoblast cells. NK cells either isolated or in the composition of mononuclear cell fraction, caused death of trophoblast cells. In women with physiological pregnancy, the cytotoxic effect of NK cells present in mononuclear cell fraction preincubated with IL-2 was lower than in nonpregnant women, who had never been pregnant previously, and in fertile women. Read More

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http://dx.doi.org/10.1007/s10517-019-04393-4DOI Listing
February 2019

Comparative Analysis of the Effects of Intravenous Administration of Placental Mesenchymal Stromal Cells and Neural Progenitor Cells Derived from Induced Pluripotent Cells on the Course of Acute Ischemic Stroke in Rats.

Bull Exp Biol Med 2019 Feb 20;166(4):558-566. Epub 2019 Feb 20.

V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.

We compared the effects of placental mesenchymal stromal cells and neural progenitor cells derived from induced human pluripotent cells after their intravenous administration to rats in 24 h after transitory occlusion of the middle cerebral artery. The therapeutic effects were evaluated by the dynamics of animal survival, body weight, neurological deficit, and the volume of infarction focus in 7, 14, 30, and 60 days after surgery. Intravenous injection of neural progenitor cells produced a therapeutic effect on the course of experimental ischemic stroke by increasing animal survival in the most acute period and accelerating compensation of neurological deficit and body weight recovery. Read More

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http://dx.doi.org/10.1007/s10517-019-04392-5DOI Listing
February 2019

Drug with Neuroprotective Properties Noopept Does Not Stimulate Cell Proliferation.

Bull Exp Biol Med 2019 Feb 20;166(4):466-468. Epub 2019 Feb 20.

V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.

Effects of Noopept (N-phenylacetyl-L-prolylglycine ethyl ester) on the relative level of proliferation marker Ki-67 and cell cycle parameters were studied in HEK293 and SH-SY5Y cell lines. The previously established multifactorial mechanism of action of the drug includes enhancement of neurotrophin NGF and BDNF expression and increase in HIF-1 activity. The possible mitogenic action of Noopept was estimated by its effect on cell proliferation. Read More

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http://dx.doi.org/10.1007/s10517-019-04373-8DOI Listing
February 2019

Blood Cytokine Profile in Rats with Different Behavioral Characteristics after Metabolic Stress.

Bull Exp Biol Med 2019 Feb 20;166(4):432-435. Epub 2019 Feb 20.

Federal Research Center of Nutrition and Biotechnology, Moscow, Russia.

Changes in the blood cytokine profile in rats with various parameters of behavior were studied under conditions of 5-day starvation not followed by the recovery period or with subsequent normalization of food intake. Metabolic stress had no effect on the content of most cytokines. The concentration of pro- and anti-inflammatory cytokines in blood plasma of rats was shown to increase significantly by the end of a 5-day recovery period after food deprivation. Read More

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http://dx.doi.org/10.1007/s10517-019-04366-7DOI Listing
February 2019

Correlations between the Levels of the Main Extracellular Matrix Components in Mouse Liver in Chronic BCG-Induced Granulomatosis.

Bull Exp Biol Med 2019 Feb 20;166(4):469-472. Epub 2019 Feb 20.

Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

Correlations between extracellular matrix components in mouse liver revealed the pathogenetically determined dynamic structure of these interrelations (some correlations appeared while others disappeared) at various terms of BCG-induced granulomatosis. The correlations between the studied parameters were strong during the first (postinfection days 3-10) and the second (postinfection days 60-90) periods but became moderate during the third period manifested by chronic inflammation (postinjection day 180). The greatest number of correlations was observed at the beginning of the stabilization period (postinjection day 60). Read More

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http://dx.doi.org/10.1007/s10517-019-04374-7DOI Listing
February 2019

Peculiarities of Immediate Response of Respiratory Chain Enzymes in Rat Cerebral Cortex to Hypoxia.

Bull Exp Biol Med 2019 Feb 20;166(4):426-431. Epub 2019 Feb 20.

Research Institute of General Pathology and Pathophysiology, Moscow, Russia.

We performed a complex study of the dependence of immediate reaction of catalytic subunits in mitochondrial enzymes (NDUFV2, SDHA, Cyt b, COX1, and ATP5A) in rat cerebral cortex (the most hypoxia-sensitive tissue) on the severity and duration of hypoxia in vivo and the role of individual resistance of rats to oxygen deficiency in this process. Three types of responses to hypoxia were revealed. The immediate response of mitochondria to oxygen deficiency appeared after its drop by 30-33% relatively to normal atmosphere level. Read More

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http://dx.doi.org/10.1007/s10517-019-04365-8DOI Listing
February 2019

Stimulation of Spermatogenesis and Synthesis of Testosterone by Allotransplantation of Neonatal Testicular Tissue under Tunica Albuginea of Cryptorchid Testis.

Bull Exp Biol Med 2019 Feb 20;166(4):497-502. Epub 2019 Feb 20.

N. A. Lopatkin Research Institute of Urology and Interventional Radiology Affiliated Branch of National Medical Research Center of Radiology, Ministry of Health of the Russian Federation, Moscow, Russia.

The abdominal type of cryptorchism was modeled on random-bred albino rats by replacing both testes from the scrotum into the abdominal cavity for 3 weeks; thereupon they were manipulated into the scrotum. In control rats, no additional surgery was performed. In experimental rats, the testicular tissue obtained from 1-2-day rat pups was transplanted under testicular tunica albuginea. Read More

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http://dx.doi.org/10.1007/s10517-019-04381-8DOI Listing
February 2019

Analysis of Anti-Glycan IgG and IgM Antibodies in Colorectal Cancer.

Bull Exp Biol Med 2019 Feb 20;166(4):489-493. Epub 2019 Feb 20.

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.

We propose an approach that allows simultaneous determination of the levels of M and G isotypes of antibodies to the panel of glycans using microarrays. The level of IgG antibodies to 3'-O-su-Le glycan detects patients with colorectal cancer with a sensitivity of 69.77% and specificity of 62. Read More

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http://dx.doi.org/10.1007/s10517-019-04379-2DOI Listing
February 2019

Carbohydrate Specificity and Isotypes of Monoclonal and Polyclonal Antibodies to Conjugated Tetrasaccharide, a Synthetic Analogue of Repeating Unit of Capsular Polysaccharide of Streptococcus Pneumoniae Serotype 14.

Bull Exp Biol Med 2019 Feb 20;166(4):477-480. Epub 2019 Feb 20.

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.

We studied carbohydrate specificity and isotypes of antibodies to BSA-conjugated tetrasaccharide, a repeating unit of the capsular polysaccharide of Streptococcus pneumoniae serotype 14, in mouse polyclonal sera and hybridoma-synthesized products. Natural IgM antibodies to the tetrasaccharide containing epitopes similar to surface carbohydrate structures of mammalian and human cells in low titers were determined in native mouse serum by ELISA using biotinylated tetrasaccharide and synthetic capsular polysaccharide as the solid-phase antigens. Polyclonal sera to the conjugated tetrasaccharide contained IgM and all subclasses of IgG antibodies, which were detected in a higher titer when the biotinylated tetrasaccharide was used as a solid phase antigen compared to synthetic capsular polysaccharide. Read More

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http://link.springer.com/10.1007/s10517-019-04376-5
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http://dx.doi.org/10.1007/s10517-019-04376-5DOI Listing
February 2019
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Analgesic Activity of Hexaazaisowurtzitane Derivatives.

Bull Exp Biol Med 2019 Feb 20;166(4):461-465. Epub 2019 Feb 20.

E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Tomsk, Russia.

Pronounced analgesic activity of the innovative compound 4-(3,4-dibromthiophencarbonyl)-2,6,8,12-tetraacethyl-2,4,6,8,10,12hexaazatetracyclo[5,5,0,0,0] dodecane (thiowurtzine) was observed in the thermal nociceptive hot plate test and in the acute visceral and somatic deep pain model (acetic acid writhing test). In these experimental models, naloxone-sensitive thiowurtzine-induced analgesia was revealed. The absence of tropism to peripheral opioid receptors in the acetic acid writhing test was demonstrated using naloxone methiodide. Read More

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http://dx.doi.org/10.1007/s10517-019-04372-9DOI Listing
February 2019

Strategy of Pharmacological Regulation of Intracellular Signal Transduction in Regeneration-Competent Cells.

Bull Exp Biol Med 2019 Feb 20;166(4):448-455. Epub 2019 Feb 20.

E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Tomsk, Russia.

The study focuses on the development of principally novel priority-oriented healthcare strategy - targeted therapy in regenerative medicine known as Strategy of Pharmacological Control over Intracellular Signal Transduction in Regeneration-Competent Cells. It implies selective action of promising drugs on specific key elements in the signaling cascade responsible for functional activity of various progenitor cells (including stem cells) and elements of tissue microenvironment. The results of pioneer studies are described that were aimed on revealing the peculiarities in signal transduction and the role of distinct signaling molecules (the potential targets) in the control of cell cycle and other functions of progenitor elements and regulatory cells of different types. Read More

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http://dx.doi.org/10.1007/s10517-019-04370-xDOI Listing
February 2019

Ligands of NOD2 (Muramyl Dipeptide) and TLR4 (LPS) in 24 h after Combined In Vivo Administration Produce a Synergistic Increase in the Content of Multipotent Stromal Cells in the Bone Marrow and Peritoneal Exudate of CBA Mice.

Bull Exp Biol Med 2019 Feb 20;166(4):473-476. Epub 2019 Feb 20.

Laboratory of Immunity Regulation and Immunological Tolerance, Moscow, Russia.

In 24 h after combined administration of ligands of NOD2 (muramyl dipeptide) and TLR4 (LPS) receptors to CBA mice, a synergistic increase (by 10 times compared to the intact control) in cloning efficiency and content of multipotent stromal cells was observed in the bone marrow in comparison with the total effects of their individual administration (by 2.1 and 4.1 times, respectively). Read More

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http://dx.doi.org/10.1007/s10517-019-04375-6DOI Listing
February 2019

Effects of Natural Cytokine Complex on the Myocardial Blood Flow in Normal and under Conditions of Increased Hemodynamic Load.

Bull Exp Biol Med 2019 Feb 20;166(4):444-447. Epub 2019 Feb 20.

M. F. Vladimirsky Moscow Regional Research Clinical Institute, Moscow, Russia.

The effects of a natural complex of cytokines IL-1, IL-2, IL-6, TNF, MIF, and GTFβ on myocardial blood flow were studied under control conditions and during acute experimental aortal stenosis. Systemic administration of the cytokine complex under control conditions led to moderate impairment of the blood flow in the myocardium associated with plethora and perivascular edema. The number of functioning vessels in the myocardium significantly increased under these conditions, which reflected enhancement of the coronary blood flow. Read More

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http://dx.doi.org/10.1007/s10517-019-04369-4DOI Listing
February 2019

Correlation between Urinary Excretion of Arginine-Vasopressin and Renal Reabsorption of Sodium and Water.

Bull Exp Biol Med 2019 Feb 20;166(4):417-420. Epub 2019 Feb 20.

I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia.

We developed an approach for quantitative assay of injected vasopressin in urine samples by ELISA under conditions of physiological suppression of hormone secretion from the neurohypophysis into the blood. In experiments on unanesthetized rats, water load (5 ml/100 g body weight) almost completely blocked secretion of arginine-vasopressin. Injection of arginine-vasopressin in a dose of 0. Read More

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http://dx.doi.org/10.1007/s10517-019-04363-wDOI Listing
February 2019

Modulation of Structural and Functional Properties of Human Lymphocytes by Reactive Oxygen Species.

Bull Exp Biol Med 2019 Feb 20;166(4):481-486. Epub 2019 Feb 20.

Voronezh State University, Voronezh, Russia.

The effects of ROS on functional properties (cytotoxic activity, antibody-producing activity, TNFα synthesis, and free cytosol calcium level), membrane structure (by expression of some surface markers), and apoptosis of lymphocytic cells were estimated in the peripheral blood of healthy volunteers. O, [Formula: see text], OH, and HO mostly suppressed cytotoxic activity of lymphocytes against Ehrlich ascites carcinoma cells and inhibited IgG synthesis and expression of receptors and surface markers (Fc receptors, CD3, CD19, and CD56). The exposure of lymphocytes to HO (10 M), O, and OH was followed by an increase in the level of a secondary messenger, intracellular calcium, in comparison with non-exposed cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04377-4DOI Listing
February 2019

Effect of Antiobiotic-Induced Disbiosis and Its Correction with Probiotics on Myocardial Tolerance to Ischemia-Reperfusion Injury in SPF Rats.

Bull Exp Biol Med 2019 Feb 20;166(4):440-443. Epub 2019 Feb 20.

Institute of Experimental Medicine, V. A. Almazov National Medical Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia.

Changes in morphofunctional parameters of the isolated heart subjected to global ischemia-reperfusion were studied in SPF Wistar rats with antibiotic-induced dysbiosis (AID) treated with lyophilized yeast Saccharomyces boulardii and inactivated probiotic bacteria Lactobacillus reuteri KR2017. In contrast to S. boulardii, correction of dysbiosis with L. Read More

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http://dx.doi.org/10.1007/s10517-019-04368-5DOI Listing
February 2019

Effect of Polyethylene Terephthalate on Functional Properties of Endothelial and Mesenchymal Cells.

Bull Exp Biol Med 2019 Feb 19;166(4):580-585. Epub 2019 Feb 19.

E. N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.

We studied the influence of vascular prostheses made of polytetrafluoroethylene and polyethylene terephthalate on the proliferation, migration, and NO production by bone marrow mesenchymal stem cells, human endothelial progenitor cells, and EA.hy926 endothelial cells, colonization of the prosthesis surface by endothelial and mesenchymal cells was also analyzed. Synthetic prostheses have a negative effect on cell proliferation and migration, while surface treatment with proteins (fibronectin or gelatin) promotes colonization of the prostheses with cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04395-2DOI Listing
February 2019

Effect of Extracellular Vesicles Formed by Multipotent Mesenchymal Stromal Cells on Irradiated Animals.

Bull Exp Biol Med 2019 Feb 19;166(4):574-579. Epub 2019 Feb 19.

Research Institute of General Pathology and Pathophysiology, Ministry of Health of the Russian Federation, Moscow, Russia.

C57Bl/6J mice were exposed to γ-radiation in a dose of 7.5 Gy. A week later, the experimental group received intravenous injection of extracellular vesicles isolated from the culture medium of human bone marrow multipotent mesenchymal stromal cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04394-3DOI Listing
February 2019

Efficiency and Prospects of Diagnosis of the Functional State of the Dental System.

Bull Exp Biol Med 2019 Feb 19;166(4):517-521. Epub 2019 Feb 19.

Federal Research Center Computer Science and Control, Russian Academy of Sciences, Moscow, Russia.

This paper presents the results of studying the effectiveness of the developed method for diagnosing the functional state of the dental system. The results indicate that the accuracy of the method is about 80%. The results of previous studies are reviewed and the prospects for diagnosis of the functional state of the dental system as a research and practical trend in dentistry are outlined. Read More

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http://dx.doi.org/10.1007/s10517-019-04385-4DOI Listing
February 2019

Effect of Different Regimens of Co-Transplantation of Hemopoietic and Mesenchymal Hemopoietic Stem Cells on the Rates of Hemopoietic Recovery in Laboratory Mice Treated with Cyclophosphamide in Sublethal Doses.

Bull Exp Biol Med 2019 Feb 19;166(4):553-557. Epub 2019 Feb 19.

A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Center of Radiology, Ministry of Health of the Russian Federation, Obninsk, Russia.

We studied the effect of mesenchymal stem cells and hemopoietic stem cells co-transplanted in different regimens (sequence and intervals between the injections within 0-48 h) on the rate of the bone marrow hemopoiesis recovery in 468 inbred mice with cytostatic aplasia caused by a single injection of cyclophosphamide in a dose of 500 mg/kg. The efficacy of stem cells was evaluated by animal survival and general physical condition, body weight dynamics, peripheral blood counts (leucocytes, platelets, and reticulocytes), total cellularity and the presence of hemopoietic stem cells in the bone marrow on day 9 after cyclophosphamide injection. The relative content of hemopoietic stem cells in the total myelokaryocyte pool was assessed by flow cytofluorometry using specific monoclonal antibodies to CD117 and CD184. Read More

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http://dx.doi.org/10.1007/s10517-019-04391-6DOI Listing
February 2019