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Zoonoses Public Health 2021 Jun 15. Epub 2021 Jun 15.

Royal (Dick) School of Veterinary Studies and The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian, UK.

Previous work identified that bacterial zoonoses (Brucella species, Coxiella burnetii and Leptospira hardjo) were present in Cameroonian pastoral cattle. To assess the characteristics of this zoonotic risk, we analyse seroprevalence of each pathogen and the associated management, herd and environmental factors in Cameroonian pastoral and dairy cattle. Cross-sectional samples included pastoralist herds in the Northwest Region (NWR n = 750) and Vina Division (VD n = 748) and small holder dairy herds in the NWR (n = 60). Read More

Vaccine 2021 Jun 11. Epub 2021 Jun 11.

The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China. Electronic address:

Infectious diseases can have a major impact on the profitability of the cattle industry. To determine the occurrence of bovine infectious diseases in China and the adoption of vaccination to control them, a national-wide questionnaire and focus group meeting were performed. The questionnaire was administered to 189 farmers including 93 dairy farmers, 80 beef cattle farmers and 16 yak farmers. Read More

Infect Immun 2021 Jun 14:IAI0015621. Epub 2021 Jun 14.

Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, 65211.

Brucellosis is one of the most common global zoonoses and is caused by facultative intracellular bacteria of the genus . Numerous studies have found that MyD88 signaling contributes to protection against , however the underlying mechanism has not been entirely defined. Here we show that MyD88 signaling in hematopoietic cells contributes both to inflammation and to control of infection . Read More

Mol Cells 2021 Jun 11. Epub 2021 Jun 11.

Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Center for Food and Bioconvergence, CALS, Seoul National University, Seoul 08826, Korea.

A recent genetic study with revealed the secretion activator gene A (SagA) as an autolysin component creating pores in the peptidoglycan (PGN) layer for the type IV secretion system (T4SS) and peptidoglycan hydrolase inhibitor A (PhiA) as an inhibitor of SagA. In this study, we determined the crystal structures of both SagA and PhiA. Notably, the SagA structure contained a PGN fragment in a space between the N- and C-terminal domains, showing the substrate-dependent hinge motion of the domains. Read More

PLoS Negl Trop Dis 2021 Jun 10;15(6):e0009500. Epub 2021 Jun 10.

Nelson Mandela African Institution for Science and Technology, Arusha, Tanzania.

Brucellosis is a priority zoonotic disease in Tanzania that causes ill-health in people and affects livestock productivity. Inadequate awareness and behavior risking transmission can impede control efforts. We conducted a cross-sectional survey of 333 livestock owners in three villages in the Kilombero district, Tanzania, to understand their awareness, knowledge and behavior associated with brucellosis. Read More

Neuroophthalmology 2021 1;45(2):131-138. Epub 2021 Mar 1.

Department of Internal Medicine, Wright State University School of Medicine, Dayton, Ohio, USA.

Horner's syndrome coexisting with an ipsilateral fourth cranial nerve palsy is a rare occurrence and likely localises to pathology in the cavernous sinus. One such case may have occurred in the 18 century affecting the renowned mathematician Leonhard Euler. A review of his biographies, eulogies, and three finely detailed facial portraits suggest that these two neuro-ophthalmic conditions, along with visual loss and a decades-long intermittent febrile illness, may have been the result of an orbital cellulitis and septic cavernous sinus thrombosis, from an underlying chronic brucellosis infection. Read More

J Spec Oper Med 2021 ;21(2):98-99

Human brucellosis can be caused by different Brucella spp. When conducting operations in a country with high rates of brucellosis, extra precautions should be taken. Appropriate personal protective measures should be used in situations when close contact with animal carcasses cannot be avoided. Read More

J Vis Exp 2021 May 23(171). Epub 2021 May 23.

Department of Spine Surgery, First Affiliated Hospital of Xinjiang Medical University;

Brucellar spondylodiscitis (BS) is the most prevalent and significant osteoarticular presentation of human Brucellosis, which is commonly manifested in pastoral communities. It is difficult to differentially diagnose and usually leads to irreversible neurologic deficits and spinal deformities. The initial diagnosis of BS is based on clinical findings and radiographic assessments, and the confirmed diagnosis should be established by the isolation of Brucella species from the blood and/or the standard tube agglutination test. Read More

J Appl Microbiol 2021 Jun 5. Epub 2021 Jun 5.

Hygiene and Preventive Medicine Department, Faculty of Veterinary Medicine, Kafrelsheikh University, P.O. 33516, Kafr El-Sheikh, Egypt.

Aims: This study aimed to identify the genotypic fingerprinting of B. melitensis biovar 3 isolates from ruminants in Kafr El-Sheikh, Egypt, to compare with other peers globally, and to highlight the epidemiology and potential causes of brucellosis control failure.

Methods And Results: A multilocus variable-number tandem-repeat analysis (MLVA 16) was carried out on 41 B. Read More

Acta Microbiol Immunol Hung 2021 Jun 2. Epub 2021 Jun 2.

2Department of Family Medicine, College of Medicine, University of Bisha, P.O. Box 1290, Bisha, Saudi Arabia.

We determined the incidence and clinical manifestations of human brucellosis from patients who attended a referral hospital in South of Saudi Arabia. A record-based retrospective study was conducted from January 2015 to December 2019 at King Abdulla Hospital, Bisha, Saudi Arabia. Information on patients' demographic characteristics, detailed records of signs and symptoms, and the laboratory findings were reviewed. Read More

Vet Res 2021 Jun 2;52(1):75. Epub 2021 Jun 2.

Laboratory of Vaccine and Antibody Engineering, Beijing Institute of Biotechnology, Beijing, China.

Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in humans and various animals. The threat of brucellosis has increased, yet currently available live attenuated vaccines still have drawbacks. Read More

Sci Rep 2021 Jun 1;11(1):11513. Epub 2021 Jun 1.

Department of Respiratory Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, People's Republic of China.

Brucellosis is one of the major public health problems in China, and human brucellosis represents a serious public health concern in Xinjiang and requires a prediction analysis to help making early planning and putting forward science preventive and control countermeasures. According to the characteristics of the time series of monthly reported cases of human brucellosis in Xinjiang from January 2008 to June 2020, we used seasonal autoregressive integrated moving average (SARIMA) method and nonlinear autoregressive regression neural network (NARNN) method, which are widely prevalent and have high prediction accuracy, to construct prediction models and make prediction analysis. Finally, we established the SARIMA((1,4,5,7),0,0)(0,1,2) model and the NARNN model with a time lag of 5 and a hidden layer neuron of 10. Read More

Genes (Basel) 2021 May 25;12(6). Epub 2021 May 25.

School of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil.

Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. Read More

Pathogens 2021 May 18;10(5). Epub 2021 May 18.

Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Str. 96a, 07743 Jena, Germany.

Brucellosis is a neglected zoonotic disease, and occupations with a high risk of infection exist. Limited information is available on brucellosis for these employees at high risk in Pakistan. A total of 459 persons with high-risk occupations, i. Read More

Trop Med Infect Dis 2021 May 13;6(2). Epub 2021 May 13.

Central Department of Microbiology, Tribhuvan University, Kirtipur 44618, Nepal.

(1) Background: Scrub typhus (ST) is endemic to Nepal. It is often underdiagnosed and misdiagnosed due to non-specific clinical presentation coupled with limited microbiological facilities, leading to adverse clinical outcomes. This study aimed to assess the seroprevalence of scrub typhus in febrile patients attending Sukraraj Tropical and Infectious Disease Hospital (STIDH), Nepal, from August 2018 to April 2019. Read More

Vet Med Sci 2021 May 31. Epub 2021 May 31.

Department of Animal Science, Faculty of agriculture, Ilam University, Ilam, Iran.

Brucellosis is a zoonotic disease causes by Brucella bacteria. So far, there is not any efficient treatment for this infectious disease in animals, although subunit vaccines can be a safe alternative. To this aim, we have designed a new chimeric OMP25-OMP31 antigen formulated in Chitosan nanoparticles and studied its protective efficiency in vivo. Read More

Res Vet Sci 2021 Jul 21;137:252-261. Epub 2021 May 21.

4 Attfield Close, Clyde, New Zealand. Electronic address:

Brucellosis caused by Brucella abortus and Brucella melitensis is endemic in the Republic of Azerbaijan but a complex mix of fiscal, political and technical constraints has impeded regulatory authority decision making for adoption of a sustainable national control programme. This paper reports a series of epidemiologic studies of the disease in animals and humans which we conducted between 2009 and 2020. A preliminary study and a subsequent larger study using vaccination of all non-pregnant female sheep and goats of breeding age and all females between 3 and 8 months with conjunctival Rev1 vaccine both recorded significant reduction in small ruminant seroprevalences. Read More

World J Clin Cases 2021 May;9(15):3649-3654

Department of Hematology and Rheumatology, The Second Affiliated Hospital of Xiamen Medical College, Xiamen 361021, Fujian Province, China.

Background: Brucellosis is a contagious bacterial disease caused by species, which is a leading zoonotic disease worldwide. Most patients with brucellosis have a clear infection source; however, our case had a rare presentation of secondary haemophagocytic lymphohistiocytosis without any epidemiological history.

Case Summary: A 50-year-old man was admitted to our hospital with a fever of unknown origin. Read More

Heliyon 2021 May 20;7(5):e07041. Epub 2021 May 20.

Center for Wildlife Management Studies, The School for Field Studies, PO Box 304, Karatu, Tanzania.

Understanding factors influencing conventional medical knowledge (CMK), general attitudes and risk perceptions of zoonotic diseases among rural residents who face risk of exposure to such diseases is important for human, livestock, and wildlife health. Focusing on Maasai from Makame, Kiteto District (Tanzania) who largely maintained a semi-nomadic lifestyle, we evaluated respondents' CMK of causes, symptoms, treatments, and prevention methods of rabies, brucellosis, and anthrax. In addition, we identified socio-demographic correlates of CMK with respect to the target zoonoses. Read More

J Family Med Prim Care 2021 Feb 27;10(2):1028-1033. Epub 2021 Feb 27.

Indira Gandhi Government Medical College and Hospital, Central Ave, Mominpura, Nagpur, Maharashtra, India.

Introduction: Brucellosis is a recognised occupational threat among animal handler and raw animal product consumers. In India, there is a likelihood of missed diagnoses and under-reporting cases by physicians causing an extended debilitating illness. We steered research to conclude the seroprevalence and risk factors allied with Human Brucellosis (HB) among the rural population in Nagpur District, Maharashtra, India. Read More

Front Pharmacol 2021 10;12:657805. Epub 2021 May 10.

Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University, Beijing, China.

We investigated the prevalence, demographic and clinical features, and risk factors associated with drug-induced liver injury (DILI) during the treatment of brucellosis inpatients in a retrospective study. We collected the clinical data of 782 brucellosis inpatients admitted at the Shawan County People's Hospital, Xinjiang, from 2015-2019. All cases were re-evaluated using the international consensus of DILI criteria and RUCAM rating scale. Read More

Medicine (Baltimore) 2021 May;100(21):e26076

The Spinal Surgery Department of The First Affiliated Hospital Of University Of South China.

Abstract: There has been no ideal surgical approach for lumbar brucella spondylitis (LBS). This study aims to compare clinical efficacy and safety of posterior versus anterior approaches for the treatment of LBS.From April 2005 to January 2015, a total of 27 adult patients with lumbar brucella spondylitis were recruited in this study. Read More

Epidemiol Infect 2021 May 25;149:e136. Epub 2021 May 25.

Department of Immunology, College of Basic Medicine, Xinjiang Medical University, Urumqi, 830011Xinjiang, China.

Brucellosis is one of the most serious and widespread zoonotic diseases, which seriously threatens human health and the national economy. This study was based on the T/B dominant epitopes of Brucella outer membrane protein 22 (Omp22), outer membrane protein 19 (Omp19) and outer membrane protein 28 (Omp28), with bioinformatics methods to design a safe and effective multi-epitope vaccine. The amino acid sequences of the proteins were found in the National Center for Biotechnology Information (NCBI) database, and the signal peptides were predicted by the SignaIP-5. Read More

Microb Pathog 2021 May 21;157:104958. Epub 2021 May 21.

Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Brucellosis during pregnancy is associated with major concerns for mothers but, it is still not clear whether the infection could be transmitted through breastfeeding to newborns. This study aimed at evaluating the shedding of Brucella melitensis through human milk. We describe phenotypic and genotypic characterization of Brucella isolate from human milk. Read More

One Health 2021 Dec 1;13:100262. Epub 2021 May 1.

World Health Organization Regional Office for the Eastern Mediterranean, Cairo, Egypt.

Background: Zoonotic diseases constitute a threat to humans and animals. The Middle East Region is a hotspot for such a threat; given its geographic location under migratory birds' flight paths, mass gatherings, political conflicts, and refugee crises. Thus, prioritizing zoonotic diseases of national significance is critical for preventing and controlling such threats and optimizing limited resources. Read More

One Health 2021 Dec 27;13:100255. Epub 2021 Apr 27.

Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.

Brucellosis is a highly contagious and incapacitating disease of humans, livestock and wildlife species globally. Treatment of brucellosis in animals is not recommended, and in humans, combinations of antibiotics recommended by the World Health Organization are used. However, sporadic antimicrobial-resistant (AMR) isolates and relapse cases have been reported from different endemic regions. Read More

Front Vet Sci 2021 7;8:630960. Epub 2021 May 7.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Brucellosis, a zoonotic disease, infects various hosts, including swine and humans. It has reemerged in recent years as a public health concern, and current studies on brucellosis infection in swine have been conducted worldwide. However, no meta-analyses of global brucellosis infection in swine have been published. Read More

J Dairy Sci 2021 May 20. Epub 2021 May 20.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G2P5. Electronic address:

The aim of this study was to explore the effect of colostrum feeding time on the ileal microbiome of neonatal calves. In this study, 22 male Holstein calves were randomly assigned to different colostrum feeding time treatments: after birth (at 45 min, n = 7); at 6 h after birth (n = 8); and at 12 h after birth (TRT12h; n = 7). At 51 h after birth, calves were killed and ileum digesta was collected for microbiome analysis using shotgun metagenomic sequencing. Read More

Microbiol Resour Announc 2021 May 20;10(20). Epub 2021 May 20.

State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

This report describes the isolation, sequencing, and annotation of Ws20160810, which was isolated from a blood sample from a brucellosis patient suffering from swelling of the right testicle in the Inner Mongolia Autonomous Region, China. The genome size was 3,244,234 bp with a 57.23% GC content, 3,294 coding DNA sequences (CDSs), 55 tRNAs, 5 rRNAs (5S [ = 2], 16S [ = 1], and 23S [ = 2]), and 3 small RNAs (sRNAs). Read More

BMC Infect Dis 2021 May 20;21(1):460. Epub 2021 May 20.

School of Animal Science and Technology, Shihezi University, Shihezi City, 832000, Xinjiang, China.

Background: This case report describes the clinical process of a shepherd who suffered brucellosis-related endocarditis (BE) and spondylitis (BS) and was infected with Brucella melitensis biovar 3 (B. melitensis biovar 3).

Case Presentation: A 55-year-old male patient was admitted to The First Affiliated Hospital of Shihezi University on October 11, 2018, due to over 3 months of intermittent fever, back pain, and heart trouble. Read More