6,632 results match your criteria Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas[Journal]


Estrogen attenuates TGF-β1-induced EMT in intrauterine adhesion by activating Wnt/β-catenin signaling pathway.

Braz J Med Biol Res 2020 6;53(8):e9794. Epub 2020 Jul 6.

College of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, China.

Although estrogen has crucial functions for endometrium growth, the specific dose and underlying molecular mechanism in intrauterine adhesion (IUA) remain unclear. In this study, we aimed to investigate the effects of estrogen on epithelial-mesenchymal transition (EMT) in normal and fibrotic endometrium, and the role of estrogen and Wnt/β-catenin signaling in the formation of endometrial fibrosis. CCK-8 and immunofluorescence assay were performed to access the proliferation of different concentrations of estrogen on normal human endometrial epithelial cells (hEECs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209794DOI Listing

Hyaluronic acid versus dexamethasone for the treatment of recurrent aphthous stomatitis in children: efficacy and safety analysis.

Braz J Med Biol Res 2020 26;53(8):e9886. Epub 2020 Jun 26.

Department of Stomatology, Jiangmen Maternal and Child Health Hospital (Jiangmen Children's Hospital), Jiangmen, Guangdong, China.

The objective of this study was to compare the safety and efficacy of 0.2% hyaluronic acid (HA) topical gel and dexamethasone topical ointment in the treatment of recurrent aphthous ulcers (RAU) in children. This retrospective observational study included 104 patients who had more than two episodes of oral aphthous ulcers per year and were treated with HA (n=52) or dexamethasone (n=52) from August 15, 2014 to September 3, 2018. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326376PMC

A method to assess heart rate variability in neonate rats: validation in normotensive and hypertensive animals.

Braz J Med Biol Res 2020 26;53(8):e9493. Epub 2020 Jun 26.

Laboratório de Fisiologia Translacional, Universidade Nove de Julho, São Paulo, SP, Brasil.

Several studies have focused on the heart rate variability (HRV) of murine species, while studies discussing HRV in murine neonates and infants remain scarce, since recording hemodynamic signals through invasive methods in small animals has been found to be quite challenging. Thus, this study aimed at describing and validating a novel method to assess HRV in newborn rats. An electrocardiogram (ECG) system was used to determine RR intervals in awake newborns and evaluate HRV in normotensive (Wistar) and hypertensive (SHR) neonate rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326379PMC

Predictors of chest drainage of pneumothorax in neonates.

Braz J Med Biol Res 2020 26;53(8):e9469. Epub 2020 Jun 26.

Department of Radiology, Children's Hospital of Soochow University, Suzhou, China.

This is a retrospective, single-center observational study to explore the predictors of chest drainage for neonatal pneumothorax. A total of 183 neonates (age ≤28 days) who presented to the Children's Hospital of Soochow University between January 1, 2015 and December 31, 2018 for pneumothorax or developed pneumothorax during a hospital stay were included. Demographic data, clinical presentation, and imaging characteristics of neonatal pneumothorax were collected and analyzed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326378PMC

Long non-coding RNA LINC01268 promotes cell growth and inhibits cell apoptosis by modulating miR-217/SOS1 axis in acute myeloid leukemia.

Braz J Med Biol Res 2020 26;53(8):e9299. Epub 2020 Jun 26.

Department of Hematology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China.

The aim of this study was to evaluate the pathogenic role of newly identified long non-coding (lnc)-RNA LINCO1268 in acute myeloid leukemia (AML), and investigate its therapeutic potential. The expression level of LINC01268 in AML was measured by quantitative PCR (qPCR). The viability, cell cycle progression, and apoptosis of AML cells were measured by CCK-8 assay and flow cytometry, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326380PMC

Treatment with zolpidem after ethanol administration potentiates the expression of ethanol-induced behavioral sensitization in mice.

Braz J Med Biol Res 2020 26;53(8):e10034. Epub 2020 Jun 26.

Departamento de Ciências da Saúde, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.

Contradictory findings suggest that the behavioral and abuse-related effects of ethanol are mediated by its action at α1 subunit-containing GABAA (α1GABAA) receptors. In the present study, we investigated the effects of a sub-chronic post-ethanol administration treatment with zolpidem, an α1-preferring positive allosteric modulator at GABAA receptors, on the subsequent expression of ethanol-induced behavioral sensitization in mice. Animals received ethanol (1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X202010034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326377PMC

Temporomandibular inflammation mobilizes parvalbumin and FosB/deltaFosB neurons of amygdala and dorsal raphe.

Braz J Med Biol Res 2020 19;53(8):e9950. Epub 2020 Jun 19.

Departamento de Psicologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.

Pathophysiological mechanisms involved in orofacial pain and their relationship with emotional disorders have emerged as an important research area for multidisciplinary studies. In particular, temporomandibular disorders (TMD) have been evaluated clinically from both physiological and psychological perspectives. We hypothesized that an altered neuronal activity occurs in the amygdala and the dorsal raphe nucleus (DR), encephalic regions involved in the modulation of painful and emotional information. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307894PMC

Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response.

Braz J Med Biol Res 2020 19;53(8):e9501. Epub 2020 Jun 19.

Department of Gynaecology and Obstetrics, Binzhou Medical University Hospital, Binzhou, Shandong, China.

Sepsis often leads to cardiac dysfunction and inflammation. This study investigated the clinical value of microRNA-328 (miR-328) in sepsis and its role in cardiac dysfunction and inflammation caused by sepsis. The expression level of miR-328 in the serum of the subjects was detected by qRT-PCR. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307893PMC

Modulation of Wnt/β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells.

Braz J Med Biol Res 2020 19;53(8):e9488. Epub 2020 Jun 19.

Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, China.

Macrophages play pivotal roles in host defense and immune homeostasis, which have two major functional polarization states, the classically activated M1 and the alternatively activated M2. Interleukin (IL)-17A is an immune modulator able to shape macrophage phenotypes. Wnt/β-catenin is a developmental signaling pathway that plays crucial roles in morphogenesis and tissue homeostasis, which has also been recently demonstrated playing roles in immune regulation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307890PMC

Subjective time under altered states of consciousness in ayahuasca users in shamanistic rituals involving music.

Braz J Med Biol Res 2020 19;53(8):e9278. Epub 2020 Jun 19.

Departamento de Psicologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.

Ayahuasca is described as a hallucinogenic substance whose property is to alter the subjective experience of time and impair the perception of the passage of time during stimuli of more than two to three seconds. The dose-dependent effects of two concentrations of ayahuasca in the ritualistic context were investigated employing temporal reproduction tasks in participants experienced in shamanistic ayahuasca rituals. The study was conducted on nine healthy volunteers who ingested two doses of ayahuasca at two times during a ritual session. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307892PMC

Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis.

Braz J Med Biol Res 2020 19;53(8):e9268. Epub 2020 Jun 19.

Laboratório de Biofísica Teórico-Experimental e Computacional, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Dois Irmãos, Recife, PE, Brasil.

Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307891PMC

Development of a three-channel automatic climbing training system for rat rehabilitation after ischemic stroke.

Braz J Med Biol Res 2020 10;53(7):e8943. Epub 2020 Jun 10.

Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan.

This paper reports the development of a three-channel automatic speed-matching climbing training system that could train three rats at the same time for rehabilitation after an ischemic stroke. An infrared (IR) remote sensor was installed at the end of each channel to monitor the real-time position of a climbing rat. This research was carried out in five stages: i) system design; ii) hardware circuit; iii) running speed control; iv) functional testing; and v) verification using an animal model of cerebral stroke. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20208943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296713PMC

Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats.

Braz J Med Biol Res 2020 3;53(7):e9628. Epub 2020 Jun 3.

Nephrology Department, Heping Hospital affiliated to Changzhi Medical College, Changzhi, Shanxi, China.

Ophiopogonin D (OP-D) is the principal pharmacologically active ingredient from Ophiopogon japonicas, which has been demonstrated to have numerous pharmacological activities. However, its protective effect against renal damage in streptozotocin (STZ)-induced diabetic nephropathy (DN) rats remains unclear. The present study was performed to investigate the protective effect of OP-D in the STZ-induced DN rat model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279694PMC

Knocking down LSD1 inhibits the stemness features of colorectal cancer stem cells.

Braz J Med Biol Res 2020 3;53(7):e9230. Epub 2020 Jun 3.

Department of Gastrointestinal Surgery, Guizhou Provincial Bijie City Qixingguan District People's Hospital, Bijie, China.

As a top leading cause of cancer death in many countries, colorectal cancer (CRC) has drawn increasing attention to the study of the pathological mechanism. According to the "cancer stem cell hypothesis", malignancies originate from a small fraction of cancer cells that show self-renewal properties to initiate and sustain tumor growth and tumor metastasis. Therefore, these cancer stem cells (CSC) probably play important roles in tumor recurrence, metastasis, and drug resistance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279696PMC

Relationship between PI3K/mTOR/RhoA pathway-regulated cytoskeletal rearrangements and phagocytic capacity of macrophages.

Braz J Med Biol Res 2020 3;53(7):e9207. Epub 2020 Jun 3.

Department of Gerontal Respiratory Medicine, The First Hospital of Lanzhou University, Lanzhou, Gansu, China.

The objective of this study was to investigate the relationship between PI3K/mTOR/RhoA signaling regulated cytoskeletal rearrangements and phagocytic capacity of macrophages. RAW264.7 macrophages were divided into four groups; blank control, negative control, PI3K-RNAi, and mTOR-RNAi. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279697PMC

MicroRNA-424-5p inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma cells by decreasing AKT3 expression.

Braz J Med Biol Res 2020 3;53(7):e9029. Epub 2020 Jun 3.

Department of Otorhinolaryngology and Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.

This study examined the expression and potential mechanism of microRNA (miRNA)-424-5p in nasopharyngeal carcinoma (NPC). NPC tissues were collected from 40 patients who were enrolled in the study, and skin samples were collected from 26 healthy subjects during plastic surgery as controls. We performed various in vitro assays using miR-424-5p to examine its function in primary NPC-1 cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279695PMC

Reaching task performance is associated to neuromuscular junction adaptations in rats with induced diabetes mellitus.

Braz J Med Biol Res 2020 3;53(7):e8763. Epub 2020 Jun 3.

Departamento de Fisioterapia, Universidade Federal de São Carlos, São Carlos, SP, Brasil.

Upper limb performance is affected by diabetes mellitus (DM). Neuromuscular junction (NMJ) is a key structure to understand the relationship between performance and morphology in DM. The aim of the study was to analyze NMJ plasticity due to DM in an animal model and its relationship with the function of forelimbs in rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20208763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279698PMC

Heart failure with preserved ejection fraction: an update on pathophysiology, diagnosis, treatment, and prognosis.

Braz J Med Biol Res 2020 5;53(7):e9646. Epub 2020 Jun 5.

Department of Cardiovascular Surgery, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Heart failure (HF) with preserved ejection fraction (HFpEF) is a clinical syndrome in which patients have symptoms and signs of HF with normal or near-normal left ventricular ejection fraction (LVEF ≥50%). Roughly half of all patients with HF worldwide have an LVEF ≥50% and nearly half have an LVEF <50%. Thanks to the increased scientific attention about the condition and improved characterization and diagnostic tools, the incidence of HF with reduced ejection fraction (HFrEF) dropped while that of HFpEF has increased by 45%. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296715PMC

Detoxification effects of long-chain versus a mixture of medium- and long-chain triglyceride-based fat emulsion on propafenone poisoning.

Braz J Med Biol Res 2020 5;53(7):e9491. Epub 2020 Jun 5.

Department of Emergency Medicine, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, China.

In the present study, we aimed to compare the detoxifying effects of two fat emulsions containing either long-chain triglyceride or a mixture of medium-chain and long-chain triglycerides in the propafenone-poisoned rat model. Rats were randomly divided into 3 groups according to the fat emulsions used: long-chain triglyceride-based fat emulsion (LL) group; medium-chain and long-chain triglyceride-based fat emulsion (ML) group; normal saline (NS) group. Propafenone was continuously pumped (velocity=70 mg/kg per h) until the mean blood pressure dropped to 50% of basal level. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296737PMC

lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma.

Braz J Med Biol Res 2020 5;53(7):e9271. Epub 2020 Jun 5.

Department of Pediatrics, The Affiliated Hospital of Yangzhou University, Yangzhou University, Jiangsu, Yangzhou, China.

Montelukast sodium is an effective and well-tolerated anti-asthmatic drug. Long non-coding RNAs (lncRNAs) are involved in the treatment of asthma. Therefore, this study aimed to investigate the effect of montelukast sodium on children with cough-variant asthma (CVA) and the role of lncRNA prostate cancer gene expression marker 1 (PCGEM1) in drug efficacy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296716PMC

Voxel-wise meta-analysis of structural changes in gray matter of Parkinson's disease patients with mild cognitive impairment.

Braz J Med Biol Res 2020 18;53(6):e9275. Epub 2020 May 18.

Department of Neurology, Affiliated Liuzhou People's Hospital of Guangxi University of Science and Technology/Liuzhou People's Hospital, Liuzhou, Guangxi, China.

Evidence from previous voxel-based morphometry (VBM) studies indicates that widespread brain regions are involved in Parkinson's disease with mild cognitive impairment (PD-MCI). However, the spatial localization reported for gray matter (GM) abnormalities is heterogeneous. The aim of the present study was to quantitatively integrate studies on GM abnormalities observed in PD-MCI in order to determine whether a pattern exists. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266500PMC

Animal models of human atherosclerosis: current progress.

Braz J Med Biol Res 2020 18;53(6):e9557. Epub 2020 May 18.

Core Facility Centre, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

Atherosclerosis retains the leading position among the causes of global morbidity and mortality worldwide, especially in the industrialized countries. Despite the continuing efforts to investigate disease pathogenesis and find the potential points of effective therapeutic intervention, our understanding of atherosclerosis mechanisms remains limited. This is partly due to the multifactorial nature of the disease pathogenesis, when several factors so different as altered lipid metabolism, increased oxidative stress, and chronic inflammation act together leading to the formation and progression of atherosclerotic plaques. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266502PMC

Correlation between amyloid deposits affecting renal compartments and glomerular filtration rate during renal biopsy in a renal amyloidosis case series.

Braz J Med Biol Res 2020 18;53(6):e8625. Epub 2020 May 18.

Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Niterói, RJ, Brasil.

Amyloidosis comprises a group of disorders that accumulate modified autologous proteins in organs, mainly the kidneys. Few studies have addressed the amyloid compartmental distribution and associated clinical outcomes. The aim of this study was to present a case series of renal amyloidosis correlating histopathological data with glomerular filtration rate (GFR) during kidney biopsy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20208625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266501PMC

Evaluation of factors for predicting risk of uveitis recurrence in Behcet's disease patients.

Braz J Med Biol Res 2020 8;53(6):e9118. Epub 2020 May 8.

Department of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.

This study aimed to investigate the predictive factors for uveitis recurrence (UR) risk in Behcet's disease (BD) patients. BD patients (n=164) with a history of uveitis were recruited, and demographic data, clinical features, and laboratory tests were recorded. Uveitis was defined as anterior uveitis, intermediate uveitis, posterior uveitis, panuveitis referring to the "International Uveitis Study Group recommendations for the evaluation of intraocular inflammatory disease". Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228548PMC

A Brazilian regional basic diet-induced chronic malnutrition drives liver inflammation with higher ApoA-I activity in C57BL6J mice.

Braz J Med Biol Res 2020 8;53(6):e9031. Epub 2020 May 8.

Laboratório da Biologia da Cicatrização Tecidual, Ontogenia e Nutrição de Tecidos, Departamento de Morfologia e Instituto de Biomedicina, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.

Malnutrition is still considered endemic in many developing countries. Malnutrition-enteric infections may cause lasting deleterious effects on lipid metabolism, especially in children living in poor settings. The regional basic diet (RBD), produced to mimic the Brazilian northeastern dietary characteristics (rich in carbohydrate and low in protein) has been used in experimental malnutrition models, but few studies have explored the effect of chronic RBD on liver function, a central organ involved in cholesterol metabolism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228546PMC

Effect of ethanol extracts of Antrodia cinnamomea on head and neck squamous cell carcinoma cell line.

Authors:
Li Liu Chen Wang

Braz J Med Biol Res 2020 8;53(6):e8694. Epub 2020 May 8.

Fuyang Vocational Technical College, Fuyang, China.

Head and neck squamous cell carcinoma (HNSCC) is one of the most common malignant tumors. Ethanol extract of Antrodia cinnamomea (EEA) has been widely studied for its health benefits including anticancer effects. The purpose of this study was to assess the effects of EEA on HNSCC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20208694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228547PMC

Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund's adjuvant-induced rheumatoid arthritis via inhibition of NF-κB.

Braz J Med Biol Res 2020 8;53(6):e9489. Epub 2020 May 8.

Department of Traditional Chinese Medical Gynecology, Wenzhou Hospital of Chinese Medicine, Wenzhou, China.

Rheumatoid arthritis (RA) is an autoimmune disease of knee joints involving pain and inflammation. Rhoifolin is a plant flavonoid known to have antioxidant and anti-inflammatory properties. This study was taken to identify the effect of rhoifolin on complete Freund's adjuvant (CFA)-induced arthritis in the rat model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233197PMC

Aging and low-intensity exercise change oxidative biomarkers in brain regions and radiographic measures of femur of Wistar rats.

Braz J Med Biol Res 2020 8;53(6):e9237. Epub 2020 May 8.

Laboratório de Neurobiologia Comparada, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

We investigated changes in oxidative biomarkers in brain regions such as brainstem, cerebellum, and cerebral cortex of 3-, 6-, 18-, 24-, and 30-month-old rats. We also assessed the effects of low-intensity exercise on these biomarkers in these regions of 6-, 18-, and 24-month-old rats that started exercise on a treadmill at 3, 15, and 21 months of age, respectively. Radiographic images of the femur were taken for all rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228549PMC

Treatment with β-elemene combined with paclitaxel inhibits growth, migration, and invasion and induces apoptosis of ovarian cancer cells by activation of STAT-NF-κB pathway.

Braz J Med Biol Res 2020 8;53(6):e8885. Epub 2020 May 8.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

In this study, we aimed to analyze the anti-cancer effects of β-elemene combined with paclitaxel for ovarian cancer. RT-qPCR, MTT assay, western blot, flow cytometry, and immunohistochemistry were used to analyze in vitro and in vivo anti-cancer effects of combined treatment of β-elemene and paclitaxel. The in vitro results showed that β-elemene+paclitaxel treatment markedly inhibited ovarian cancer cell growth, migration, and invasion compared to either paclitaxel or β-elemene treatment alone. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20208885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228545PMC

High levels of chemerin associated with variants in the NOS3 and APOB genes in rural populations of Ouro Preto, Minas Gerais, Brazil.

Braz J Med Biol Res 2020 8;53(6):e9113. Epub 2020 May 8.

Núcleo de Pesquisa em Ciências Biológicas, Programa de Pós-Graduação em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.

Chemerin is an adipokine that has been associated with components of metabolic syndrome. It has been described to affect adipocyte metabolism and inflammatory responses in adipose tissue, as well as the systemic metabolism of lipids and glucose. Few epidemiological studies have evaluated classical and genetics cardiovascular risk factors (CVRFs) in the mixed adult rural population in Brazil. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228550PMC

Notoginsenoside R1 upregulates miR-221-3p expression to alleviate ox-LDL-induced apoptosis, inflammation, and oxidative stress by inhibiting the TLR4/NF-κB pathway in HUVECs.

Braz J Med Biol Res 2020 8;53(6):e9346. Epub 2020 May 8.

Department of Cardiology, Central Hospital of Yiwu, Yiwu, Zhejiang, China.

Atherosclerosis (AS) is a common vascular disease, which can cause apoptosis of vascular endothelial cells. Notoginsenoside R1 (NGR1) is considered an anti-AS drug. MicroRNAs (miRNAs) are believed to play a vital role in cell apoptosis and angiogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233198PMC
June 2020
1.034 Impact Factor

miR-378a-5p and miR-630 induce lens epithelial cell apoptosis in cataract via suppression of E2F3.

Braz J Med Biol Res 2020 27;53(5):e9608. Epub 2020 Apr 27.

Department of Ophthalmology, People's Hospital of Zhaoyuan City, Zhaoyuan, Shandong, China.

Cataract, an eye disease that threatens the health of millions of people, brings about severe economic burden for patients and society. MicroRNA (miR)-378a-5p and miR-630 were recognized as essential regulators in multiple cancers. However, the exact functions of miR-378a-5p and miR-630 in cataract are still unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197652PMC
June 2020
1.034 Impact Factor

Enhanced anti-tumor efficacy of 5-aminolevulinic acid-gold nanoparticles-mediated photodynamic therapy in cutaneous squamous cell carcinoma cells.

Braz J Med Biol Res 2020 27;53(5):e8457. Epub 2020 Apr 27.

Department of Dermatology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

The objective of this study was to investigate whether the conjugation of gold nanoparticles (GNPs) to 5-aminolevulinic acid (5-ALA) could enhance the anti-tumor efficiency of photodynamic therapy (PDT) in epidermoid carcinoma cells. The mRNA and protein expression levels were determined by quantitative real-time PCR and western blot, respectively. Cell viability, apoptosis, invasion, and migration were determined by MTT assay, flow cytometry, transwell invasion assay, and migration assay, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20208457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205413PMC

Indomethacin attenuates mechanical allodynia during the organization but not the maintenance of the peripheral neuropathic pain induced by nervus ischiadicus chronic constriction injury.

Braz J Med Biol Res 2020 27;53(5):e9255. Epub 2020 Apr 27.

Laboratório de Neurociências da Dor & Emoções, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.

The neurochemical mechanisms underlying neuropathic pain (NP) are related to peripheral and central sensitization caused by the release of inflammatory mediators in the peripheral damaged tissue and ectopic discharges from the injured nerve, leading to a hyperexcitable state of spinal dorsal horn neurons. The aim of this work was to clarify the role played by cyclooxygenase (COX) in the lesioned peripheral nerve in the development and maintenance of NP by evaluating at which moment the non-steroidal anti-inflammatory drug indomethacin, a non-selective COX inhibitor, attenuated mechanical allodynia after placing one loose ligature around the nervus ischiadicus, an adaptation of Bennett and Xie's model in rodents. NP was induced in male Wistar rats by subjecting them to chronic constriction injury (CCI) of the nervus ischiadicus, placing one loose ligature around the peripheral nerve, and a sham surgery (without CCI) was used as control. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205414PMC

Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells.

Braz J Med Biol Res 2020 27;53(5):e9331. Epub 2020 Apr 27.

Department of Prevention Medicine, Hangzhou Normal University School of Medicine, Hangzhou, Zhejiang, China.

The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co-exposure in human kidney 293 cells using the MTT assay. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205411PMC

miR-22 and cerebral microbleeds in brainstem and deep area are associated with depression one month after ischemic stroke.

Braz J Med Biol Res 2020 27;53(5):e9162. Epub 2020 Apr 27.

Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

In this study, we aimed to explore the relationship among miR-22, deep cerebral microbleeds (CMBs), and post-stroke depression (PSD) 1 month after ischemic stroke. We consecutively recruited 257 patients with first-ever and recurrent acute cerebral infarction and performed PSD diagnosis in accordance with the Diagnostic and Statistical Manual IV criteria for depression. Clinical information, assessments of stroke severity, and imaging data were recorded on admission. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197650PMC

Effect of Camellia sinensis teas on left ventricular hypertrophy and insulin resistance in dyslipidemic mice.

Braz J Med Biol Res 2020 27;53(5):e9303. Epub 2020 Apr 27.

Faculdade de Medicina, Universidade José do Rosário Vellano, Alfenas, MG, Brasil.

The control of dyslipidemia using plants is an important subject of studies since it has numerous benefits in cardiovascular protection. The objective of this study was to evaluate the effect of three Camellia sinensis L. teas (green, red, and white) on left ventricular hypertrophy and insulin resistance in low-density lipoprotein receptor knockout (LDLr-/-) mice fed a high-fat diet. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197649PMC

Expression of CHPF modulates cell proliferation and invasion in lung cancer.

Braz J Med Biol Res 2020 27;53(5):e9021. Epub 2020 Apr 27.

Department of Oncology, Nanjing Medical University, Nanjing, Jiangsu, China.

Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431x20209021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205412PMC

MicroRNA-4290 suppresses PDK1-mediated glycolysis to enhance the sensitivity of gastric cancer cell to cisplatin.

Braz J Med Biol Res 2020 17;53(5):e9330. Epub 2020 Apr 17.

Department of Gastroenterology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu, China.

The development of chemotherapy resistance significantly impairs the efficiency of chemotherapy, but the underlying mechanisms of chemotherapy resistance in gastric cancer (GC) are complicated and still need to be further explored. Here, we aimed to reveal the effects of miR-4290/PDK1 (pyruvate dehydrogenase kinase 1) axis on chemotherapy resistance of GC in vitro. The expression patterns of miR-4290 in GC tissues and cell lines were determined by real-time quantitative PCR. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184963PMC

Tissue fusion technology versus suture and staple in porcine bowel anastomosis: an in vivo study.

Braz J Med Biol Res 2020 17;53(5):e9305. Epub 2020 Apr 17.

Department of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.

The aim of this study was to make a comparison between the tissue fusion technique and conventional methods for sealing bowel anastomosis. Eighteen female domestic pigs (Suidae, Sus) were used in our study. Tissue-fused anastomoses (LigaSure groups) were made in 13 animals (5 anastomoses per animal), which were subdivided into 4 groups according to different manufacturing settings: "LigaSure-L-1" and "LigaSure-L-2", with low energy output level with 1 or 2 device-activated tissue sealing times, and "LigaSure-M" and "LigaSure-H", with medium or high energy output level. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184961PMC

MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL.

Braz J Med Biol Res 2020 17;53(5):e9292. Epub 2020 Apr 17.

Dermatology Department, The First Hospital of Changsha City, Kaifu District, Changsha, China.

Systemic lupus erythematosus (SLE) is an autoimmune disease associated with an aberrant activation of immune cells partly due to the dysfunction of cytokines such as type I interferons (IFNs). Long non-coding RNA MALAT1 has been found to play a pathogenic role in SLE; however, the underlying mechanisms are still poorly understood. Bioinformatics analysis showed the up-regulation of type I IFN downstream effectors OAS2, OAS3, and OASL (OAS-like) in CD4+ T cells, CD19+ B cells, and CD33+ myeloid cells in patients with active SLE compared to healthy participants. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184960PMC

Alanyl-glutamine protects the intestinal barrier function in trained rats against the impact of acute exhaustive exercise.

Braz J Med Biol Res 2020 17;53(5):e9211. Epub 2020 Apr 17.

Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.

Strenuous exercise triggers deleterious effects on the intestinal epithelium, but their mechanisms are still uncertain. Here, we investigated whether a prolonged training and an additional exhaustive training protocol alter intestinal permeability and the putative effect of alanyl-glutamine (AG) pretreatment in this condition. Rats were allocated into 5 different groups: 1) sedentary; 2 and 3) trained (50 min per day, 5 days per week for 12 weeks) with or without 6 weeks oral (1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184964PMC

Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats.

Braz J Med Biol Res 2020 17;53(5):e9108. Epub 2020 Apr 17.

Programa de Pós-Graduação em Ciências da Motricidade, Universidade Estadual Paulista, Presidente Prudente, SP, Brasil.

Recent evidence suggests that aerobic physical training may attenuate the deleterious effects of cancer risk factors, including smoking. We investigated the effects of cigarette smoke inhalation and aerobic physical training on the expression of steroid receptors and inflammatory and apoptotic proteins in the prostate. Forty male Wistar rats were distributed in four groups: control (CO), exercise (EXE), cigarette smoke exposure (CS), and cigarette smoke exposure with exercise (CS+EXE). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184962PMC

Prognostic value of increased expression of RBM8A in gastric cancer.

Braz J Med Biol Res 2020 9;53(4):e9290. Epub 2020 Apr 9.

Department of Radiotherapy, Lishui Municipal Central Hospital, Zhejiang, China.

This study was designed to investigate the expression of RBM8A protein in patients with gastric cancer (GC) and to explore its correlation with clinical pathological features as well as prognosis. One hundred pairs of gastric carcinoma tissues and adjacent tissues from patients undergoing gastrectomy for GC were included in this study. The protein expression level of RBM8A was determined by immunohistochemistry using tissue microarrays. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164227PMC

lncRNA ZEB1-AS1 inhibits high glucose-induced EMT and fibrogenesis by regulating the miR-216a-5p/BMP7 axis in diabetic nephropathy.

Braz J Med Biol Res 2020 9;53(4):e9288. Epub 2020 Apr 9.

Department of Nephrology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China.

Diabetic nephropathy (DN) is one of the leading causes of mortality in diabetic patients. Long non-coding RNA zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) plays a crucial role in the development of various diseases, including DN. However, the molecular mechanism of ZEB1-AS1 in DN pathogenesis remains elusive. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162581PMC

A poor prognosis in human hepatocellular carcinoma is associated with low expression of DPP4.

Braz J Med Biol Res 2020 9;53(4):e9114. Epub 2020 Apr 9.

Department of Hepatobiliary Surgery, The First Hospital of Jiaxing, Jiaxing, Zhejiang, China.

This study aimed to explore the prognostic role of dipeptidyl peptidase 4 (DPP4) expression in hepatocellular carcinoma (HCC). DPP4 expression was measured in formalin-fixed paraffin-embedded specimens that were gathered from 327 HCC patients. Immunohistochemistry analyses were utilized to examine DPP4 expression characteristics and prognostic values (overall survival (OS) and time to recurrence) of DDP4 in HCC tissues. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20209114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162587PMC

VPA/PLGA microfibers produced by coaxial electrospinning for the treatment of central nervous system injury.

Braz J Med Biol Res 2020 9;53(4):e8993. Epub 2020 Apr 9.

Laboratório de Hematologia e Células-tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

The central nervous system shows limited regenerative capacity after injury. Spinal cord injury (SCI) is a devastating traumatic injury resulting in loss of sensory, motor, and autonomic function distal from the level of injury. An appropriate combination of biomaterials and bioactive substances is currently thought to be a promising approach to treat this condition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20208993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162582PMC

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition.

Braz J Med Biol Res 2020 31;53(4):e8882. Epub 2020 Mar 31.

Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.

Hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20198882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162583PMC

Acute effect of passive cycle-ergometry and functional electrical stimulation on nitrosative stress and inflammatory cytokines in mechanically ventilated critically ill patients: a randomized controlled trial.

Braz J Med Biol Res 2020 9;53(4):e8770. Epub 2020 Apr 9.

Departamento de Fisioterapia e Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, Brasil.

Early mobilization is beneficial for critically ill patients because it reduces muscle weakness acquired in intensive care units. The objective of this study was to assess the effect of functional electrical stimulation (FES) and passive cycle ergometry (PCE) on the nitrous stress and inflammatory cytometry in critically ill patients. This was a controlled, randomized, open clinical trial carried out in a 16-bed intensive care unit. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/1414-431X20208770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162584PMC