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    6805 results match your criteria Brazilian Journal of Medical and Biological Research [Journal]

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    Ultra-short-term heart rate variability during resistance exercise in the elderly.
    Braz J Med Biol Res 2018 21;51(6):e6962. Epub 2018 May 21.
    Departamento de Fisioterapia, Pós Graduação em Fisioterapia, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
    Despite the appeal of ultra-short-term heart rate variability (HRV) methods of analysis applied in the clinical and research settings, the number of studies that have investigated HRV by analyzing R-R interval (RRi) recordings shorter than 5 min is still limited. Moreover, ultra-short-term HRV analysis has not been extensively validated during exercise and, currently, no indications exist for its applicability during resistance exercise. The aim of the present study was to compare ultra-short-term HRV analysis with standard short-term HRV analysis during low-intensity, dynamic, lower limb resistance exercise in healthy elderly subjects. Read More

    Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees.
    Braz J Med Biol Res 2018 21;51(6):e7118. Epub 2018 May 21.
    Programa de Pós-graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
    In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. Read More

    Treatment with Ilex paraguariensis (yerba mate) aqueous solution prevents hepatic redox imbalance, elevated triglycerides, and microsteatosis in overweight adult rats that were precociously weaned.
    Braz J Med Biol Res 2018 21;51(6):e7342. Epub 2018 May 21.
    Laboratório de Fisiologia Endócrina, Departamento de Ciências Fisiológicas Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
    Early weaning (EW) leads to overweight, visceral obesity, hyperleptinemia, and insulin resistance in adulthood. Treatment with Ilex paraguariensis (yerba mate) improves obesity and insulin resistance in these animals. Here, we evaluated the effects of chronic treatment with yerba mate on the redox balance and liver morphology of overweight early-weaned rats. Read More

    microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb.
    Braz J Med Biol Res 2018 21;51(6):e6452. Epub 2018 May 21.
    Department of Gastroenterology, Jining No. 1 People's Hospital, Jining, China.
    Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Read More

    Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats.
    Braz J Med Biol Res 2018 21;51(6):e6602. Epub 2018 May 21.
    Laboratório de Nutrição, Atividade Física e Plasticidade Fenotípica Núcleo de Educação Física e Ciências do Esporte, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil.
    The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. Read More

    Efficacy of cognitive behavioral therapy in children and adolescents with insomnia: a systematic review and meta-analysis.
    Braz J Med Biol Res 2018 21;51(6):e7070. Epub 2018 May 21.
    Department of Neurology, Chengdu Fifth People's Hospital, Chengdu, China.
    Insomnia is highly prevalent in children and adolescents. However, the efficacy of cognitive behavioral therapy for insomnia (CBT-i) in children and adolescents remains controversial. Therefore, this systematic review and meta-analysis aimed to assess the efficacy of CBT-i in children and adolescents. Read More

    Pathogenesis of gallbladder adenomyomatosis and its relationship with early-stage gallbladder carcinoma: an overview.
    Braz J Med Biol Res 2018 21;51(6):e7411. Epub 2018 May 21.
    Department of Biliary and Minimally Invasive Surgery, China Medical University Shengjing Hospital Shenyang, Liaoning, China.
    The exact pathogenesis of gallbladder adenomyomatosis is still lacking and some controversies over its diagnosis and treatment exist. Originally recognized as a precancerous lesion, adenomyomatosis is currently recognized by recent studies as a benign alteration of the gallbladder that is often associated with cholecystitis and cholecystolithiasis. Gallbladder carcinoma is an extremely malignant disease with a 5-year survival rate of less than 5%. Read More

    Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols.
    Braz J Med Biol Res 2018 21;51(6):e7221. Epub 2018 May 21.
    Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
    Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. Read More

    lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line.
    Braz J Med Biol Res 2018 21;51(6):e7046. Epub 2018 May 21.
    Department of Radioimmunoassay, Liaocheng People's Hospital, Liaocheng, Shandong, China.
    Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. Read More

    Non-invasive messenger RNA transcriptional evaluation in human kidney allograft dysfunction.
    Braz J Med Biol Res 2018 17;51(7):e6904. Epub 2018 May 17.
    Faculdade de Medicina, Programa de Pós-Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
    The aim of the present study was to evaluate messenger RNA expression in kidney allograft recipients. Forty-four kidney transplant recipients were evaluated up to three months after grafting. After transplantation, peripheral blood samples were drawn sequentially for real-time polymerase chain reaction analyses of perforin and TIM-3 genes. Read More

    MicroRNA-451a, microRNA-34a-5p, and microRNA-221-3p as predictors of response to antidepressant treatment.
    Braz J Med Biol Res 2018 17;51(7):e7212. Epub 2018 May 17.
    Department of Psychiatry and Mental Health Center, West China Hospital, Sichuan University, Chengdu, China.
    Aberrant expression of microRNAs (miRNAs) has been shown to be involved in early observations of depression. The aim of this study was to determine if serum levels of miRNA-451a, miRNA-34a-5p, and miRNA-221-3p can serve as indicators of disease progression or therapeutic efficacy in depression. We collected data from 84 depressed patients and 78 control volunteers recruited from the medical staff at the West China Hospital. Read More

    Association study of AFF1 rs340630 polymorphism with genetic susceptibility to rheumatoid arthritis in Chinese population.
    Braz J Med Biol Res 2018 17;51(7):e7126. Epub 2018 May 17.
    Department of Orthopaedics, Liu'an People's Hospital, Liu'an, Anhui, China.
    This study was performed to examine whether the AF4/FMR2 family, member 1 (AFF1) rs340630 polymorphism is involved in the genetic background of rheumatoid arthritis (RA) in a Chinese population. Two different study groups of RA patients and controls (328 RA patients and 449 healthy controls in the first study group; 232 RA patients and 313 controls in the second study group) were included in our study. Overall, there was no significant difference in either genotype (P=0. Read More

    Hypoxia-induced hyperpermeability of rat glomerular endothelial cells involves HIF-2α mediated changes in the expression of occludin and ZO-1.
    Braz J Med Biol Res 2018 17;51(7):e6201. Epub 2018 May 17.
    Department of Nephrology, Hospital of Qinghai University, Xining, China.
    This study aimed to investigate the role of hypoxia-inducible factor-2α (HIF-2α) in the expression of tight junction proteins and permeability alterations in rat glomerular endothelial cells (rGENCs) under hypoxia conditions. The expression level of HIF-2α and tight junction proteins (occludin and ZO-1) in rGENCs were examined following 5% oxygen density exposure at different treatment times. HIF-2α lentivirus transfection was used to knockdown HIF-2α expression. Read More

    Protective effect and mechanism of Lactobacillus on cerebral ischemia reperfusion injury in rats.
    Braz J Med Biol Res 2018 17;51(7):e7172. Epub 2018 May 17.
    Cerebrovascular Disease Treatment Center, No.5 Tianjin Center Hospital, Tianjin, China.
    The present study was designed to investigate the protective effects and mechanism of inactivated lactobacillus (ILA) on cerebral ischemia reperfusion injury (CIRI) in rats. In this experiment, 30 male Sprague Dawley rats were randomly divided into control group, IRI groups, and ILA group. A middle cerebral artery occlusion and reperfusion model was prepared. Read More

    Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients.
    Braz J Med Biol Res 2018 17;51(7):e6830. Epub 2018 May 17.
    Neurosurgery Intensive Care Unit, Weifang People's Hospital, Weifang, China.
    This study aimed to investigate the risk factors related to ventilator-acquired pneumonia (VAP) in aneurysmal subarachnoid hemorrhage (SAH) patients. From January 2011 to December 2015, a single-center retrospective study including 200 SAH patients requiring mechanical ventilation (MV) ≥48 h was performed. The clinical data of these patients were collected and analyzed. Read More

    What we know and do not know about women and kidney diseases; Questions unanswered and answers unquestioned: Reflection on World Kidney Day and International Woman's Day.
    Braz J Med Biol Res 2018 17;51(7):e7315. Epub 2018 May 17.
    Department of Medicine, Division of Nephrology, University of British Columbia, Vancouver, British Columbia, Canada.
    Chronic kidney disease affects approximately 10% of the world's adult population: it is within the top 20 causes of death worldwide, and its impact on patients and their families can be devastating. World Kidney Day and International Women's Day in 2018 coincide, thus offering an opportunity to reflect on the importance of women's health and specifically women's kidney health on the community and the next generations, as well as to strive to be more curious about the unique aspects of kidney disease in women so that we may apply those learnings more broadly. Girls and women, who make up approximately 50% of the world's population, are important contributors to society and their families. Read More

    Epoetin alfa resistance in hemodialysis patients with chronic kidney disease: a longitudinal study.
    Braz J Med Biol Res 2018 7;51(7):e7288. Epub 2018 May 7.
    Departamento de Saúde Pública, Universidade Federal do Maranhão, São Luís, MA, Brasil.
    Anemia is an inevitable complication of hemodialysis, and the primary cause is erythropoietin deficiency. After diagnosis, treatment begins with an erythropoiesis-stimulating agent (ESA). However, some patients remain anemic even after receiving this medication. Read More

    Efficacy, acceptability, and tolerability of antidepressant treatments for patients with post-stroke depression: a network meta-analysis.
    Braz J Med Biol Res 2018 7;51(7):e7218. Epub 2018 May 7.
    Affiliated Liuzhou People's Hospital, Department of Neurology, Guangxi University of Science and Technology, Liuzhou, Guangxi, China.
    The aim of this study was to investigate the efficacy, acceptability, and tolerability of antidepressants in treating post-stroke depression (PSD) by performing a network meta-analysis of randomized controlled trials of the current literature. Eligible studies were retrieved from online databases, and relevant data were extracted. The primary outcome was efficacy as measured by the mean change in overall depressive symptoms. Read More

    Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis.
    Braz J Med Biol Res 2018 7;51(7):e7220. Epub 2018 May 7.
    Key Laboratory of Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.
    An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2. Read More

    miR-27a protects human mitral valve interstitial cell from TNF-α-induced inflammatory injury via up-regulation of NELL-1.
    Braz J Med Biol Res 2018 19;51(6):e6997. Epub 2018 Apr 19.
    Department of Cardiology, Chengyang People's Hospital, Qingdao, Shandong, China.
    MicroRNAs (miRNAs) have been reported to be associated with heart valve disease, which can be caused by inflammation. This study aimed to investigate the functional impacts of miR-27a on TNF-α-induced inflammatory injury in human mitral valve interstitial cells (hMVICs). hMVICs were subjected to 40 ng/mL TNF-α for 48 h, before which the expressions of miR-27a and NELL-1 in hMVICs were altered by stable transfection. Read More

    Quality of life on hemodialysis and inflammation: a descriptive analysis.
    Braz J Med Biol Res 2018 19;51(6):e7355. Epub 2018 Apr 19.
    Departamento de Clínica Médica, Faculdade de Medicina ABC, Santo André, SP, Brasil.
    Chronic kidney disease (CKD) is highly prevalent worldwide. Patients with CKD on hemodialysis are more likely to present behavioral changes and worse quality of life as a result of their routine and complications. They also have higher levels of cytokines. Read More

    Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury.
    Braz J Med Biol Res 2018 23;51(6):e6555. Epub 2018 Apr 23.
    Department of Cardiology, Dezhou People's Hospital, Dezhou, China.
    Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of cardiovascular diseases, especially in myocardial infarction and ischemia/reperfusion (I/R). However, the underlying molecular mechanism remains unclear. In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. Read More

    Exploration of gene functions for esophageal squamous cell carcinoma using network-based guilt by association principle.
    Braz J Med Biol Res 2018 19;51(6):e6801. Epub 2018 Apr 19.
    Department of Gastroenterology (40th Ward), Daqing Oilfield General Hospital, Daqing, China.
    Gene networks have been broadly used to predict gene functions based on guilt by association (GBA) principle. Thus, in order to better understand the molecular mechanisms of esophageal squamous cell carcinoma (ESCC), our study was designed to use a network-based GBA method to identify the optimal gene functions for ESCC. To identify genomic bio-signatures for ESCC, microarray data of GSE20347 were first downloaded from a public functional genomics data repository of Gene Expression Omnibus database. Read More

    Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression.
    Braz J Med Biol Res 2018 19;51(6):e7238. Epub 2018 Apr 19.
    Laboratory of Pharmacology, Department of Health Sciences, Division of Health and Biological Sciences, Metropolitan Autonomous University of Iztapalapa, Mexico City, Mexico.
    Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Read More

    Andrographolide protects mouse astrocytes against hypoxia injury by promoting autophagy and S100B expression.
    Braz J Med Biol Res 2018 19;51(6):e7061. Epub 2018 Apr 19.
    Department of Anesthesiology, Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
    Andrographolide (ANDRO) has been studied for its immunomodulation, anti-inflammatory, and neuroprotection effects. Because brain hypoxia is the most common factor of secondary brain injury after traumatic brain injury, we studied the role and possible mechanism of ANDRO in this process using hypoxia-injured astrocytes. Mouse cortical astrocytes C8-D1A (astrocyte type I clone from C57/BL6 strains) were subjected to 3 and 21% of O2 for various times (0-12 h) to establish an astrocyte hypoxia injury model in vitro. Read More

    Protective effect of yerba mate intake on the cardiovascular system: a post hoc analysis study in postmenopausal women.
    Braz J Med Biol Res 2018 19;51(6):e7253. Epub 2018 Apr 19.
    Departamento de Clínica Médica, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
    The prevalence of cardiovascular and metabolic diseases is increased in postmenopausal women, which contributes to the burden of illnesses in this period of life. Yerba mate (Ilex paraguariensis) is a native bush from Southern South America. Its leaves are rich in phenolic components, which may have antioxidant, vasodilating, hypocholesterolemic, and hypoglycemic proprieties. Read More

    Dietary intake and eating behavior in depot medroxyprogesterone acetate users: a systematic review.
    Braz J Med Biol Res 2018 23;51(6):e7575. Epub 2018 Apr 23.
    Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, IN, USA.
    Because of weight gain, women often discontinue hormonal contraception, especially depot medroxyprogesterone acetate (DMPA). Our objective was to conduct a systematic review of studies describing dietary intake or eating behavior in DMPA users to understand whether the use of DMPA is associated with changes in dietary habits and behaviors leading to weight gain. We searched the PubMed, POPLINE, CENTRAL Cochrane, Web of Science, and EMBASE databases for reports published in English between 1980 and 2017 examining dietary intake or eating behavior in healthy women in reproductive age and adolescents using DMPA (150 mg/mL). Read More

    TNF-α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal.
    Braz J Med Biol Res 2018 23;51(6):e7065. Epub 2018 Apr 23.
    Department of Pathology, School of Basic Medicine, Medical College of Qingdao University, Qingdao, Shandong, China.
    Ulcerative colitis is a chronic inflammatory disease of the colon where intestinal motility is disturbed. Interstitial cells of Cajal (ICC) are required to maintain normal intestinal motility. In the present study, we assessed the effect of tumor necrosis factor-alpha (TNF-α) on viability and apoptosis of ICC, as well as on the expression of stem cell factor (SCF), ghrelin, and substance P. Read More

    Influence of expiratory positive airway pressure on cardiac autonomic modulation at rest and in submaximal exercise in COPD patients.
    Braz J Med Biol Res 2018 19;51(6):e7180. Epub 2018 Apr 19.
    Curso de Fisioterapia, Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul, Santa Cruz do Sul, RS, Brasil.
    The aim of this study was to evaluate the effect of expiratory positive airway pressure (EPAP) on heart rate variability (HRV) indices at rest and during 6-min walk test (6MWT) in chronic obstructive pulmonary disease (COPD) patients. Fifteen moderate to severe COPD patients were randomized and evaluated with and without (Non-EPAP) a 5 cmH2O EPAP device. Respiratory rate (RR) was collected at rest (5 min), during the 6MWT (5 min), and at recovery (5 min). Read More

    Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats.
    Braz J Med Biol Res 2018 19;51(6):e6982. Epub 2018 Apr 19.
    Laboratório de Fisiologia Endócrina, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
    Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Read More

    LncRNA HULC mediates radioresistance via autophagy in prostate cancer cells.
    Braz J Med Biol Res 2018 23;51(6):e7080. Epub 2018 Apr 23.
    Tengzhou Central People's Hospital, Department of Urology, Jining Medical College, Tengzhou, China.
    Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10-45% of PCa are resistant to irradiation. We aimed to explore the role of long non-coding RNA highly upregulated in liver cancer (HULC) in the sensitivity of PCa cells to irradiation. Read More

    PCR-RFLP assay as an option for primary HPV test.
    Braz J Med Biol Res 2018 Mar 26;51(5):e7098. Epub 2018 Mar 26.
    Laboratório de Biologia Molecular, Microbiologia e Sorologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
    Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. Read More

    Performance during a strenuous swimming session is associated with high blood lactate: pyruvate ratio and hypoglycemia in fasted rats.
    Braz J Med Biol Res 2018 Mar 26;51(5):e7057. Epub 2018 Mar 26.
    Departamento de Farmacologia e Terapêutica, Universidade Estadual de Maringá, Maringá, PR, Brasil.
    The aim of this study was to investigate the effect of lactatemia elevation and glycemia reduction on strenuous swimming performance in fasted rats. Three rats were placed in a swimming tank at the same time. The first rat was removed immediately (control group) and the remaining ones were submitted to a strenuous swimming session. Read More

    Effect of pre-exercise carbohydrate availability on fat oxidation and energy expenditure after a high-intensity exercise.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6964. Epub 2018 Mar 26.
    Grupo de Pesquisa em Ciências do Esporte, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil.
    The aim of this study was to test the hypothesis that reduced pre-exercise carbohydrate (CHO) availability potentiates fat oxidation after an exhaustive high-intensity exercise bout. Eight physically active men underwent a high-intensity exercise (∼95% V̇O2max) until exhaustion under low or high pre-exercise CHO availability. The protocol to manipulate pre-exercise CHO availability consisted of a 90-min cycling bout at ∼70% V̇O2max + 6 × 1-min at 125% V̇O2max with 1-min rest, followed by 48 h under a low- (10% CHO, low-CHO availability) or high-CHO diet (80% CHO, high-CHO availability). Read More

    Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6773. Epub 2018 Mar 26.
    Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista, BA, Brasil.
    Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. Read More

    Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6754. Epub 2018 Mar 26.
    Laboratório de Separação por Membranas, Departamento de Engenharia Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
    Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on human dental pulp stem cell detachment. Read More

    Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6690. Epub 2018 Mar 26.
    Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.
    Trypanosoma cruzi triggers a progressive inflammatory response affecting cardiovascular functions in humans and experimental models. Angiotensin II, a key effector of the renin-angiotensin system, plays roles in mediating hypertension, heart failure, and inflammatory responses. T. Read More

    Unsteady walking as a symptom in type 2 diabetes mellitus: independent association with depression and sedentary lifestyle and no association with diabetic neuropathy.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6605. Epub 2018 Mar 26.
    Programa de Pós-Graduação em Ciências da Saúde, Ginecologia e Obstetrícia, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
    The purpose of this study was to look at the determinants of the unsteady walking (UW) symptom in patients with type 2 diabetes mellitus (T2DM) by defining if UW and/or the Diabetic Neuropathy Symptoms Score (DNSS) are associated with positive scores in Beck's Depression Inventory (BDI) and with a positive Michigan Neuropathy Screening Instrument score (MNSI). We evaluated 203 T2DM patients without visible gait disturbances. They were divided into UW (+) and UW (-) or DNSS (+) and DNSS (-) according to symptoms. Read More

    Use of cardiopulmonary exercise testing to assess early ventilatory changes related to occupational particulate matter.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6486. Epub 2018 Mar 26.
    Laboratório de Epidemiologia e Movimento Humano, Departamento de Ciências do Movimento Humano, Universidade Federal de São Paulo, Santos, SP, Brasil.
    Spirometry has been used as the main strategy for assessing ventilatory changes related to occupational exposure to particulate matter (OEPM). However, in some cases, as one of its limitations, it may not be sensitive enough to show abnormalities before extensive damage, as seen in restrictive lung diseases. Therefore, we hypothesized that cardiopulmonary exercise testing (CPET) may be better than spirometry to detect early ventilatory impairment caused by OEPM. Read More

    Xanthine oxidase activity in patients with age-related cataract associated with hypertension.
    Braz J Med Biol Res 2018 Mar 26;51(5):e6129. Epub 2018 Mar 26.
    Department for Laboratory Investigation, Railway Health Care Institute, Novi Sad, Serbia.
    Reactive oxygen species and lipid peroxidation are important factors that contribute to the development of age-related cataract. The study included 130 patients with age-related cataract, 69 of whom were diagnosed with hypertension (HT), 20 with hypertension and type 2 diabetes mellitus (DM), and 41 had no accompanying condition. The following parameters were measured in the serum of the examinees: products of lipid peroxidation malondialdehyde (MDA) and lipofuscin-like fluorophores (LLF), activity of prooxidative enzymes xanthine oxidase (XO) and myeloperoxidase (MPO), antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), the concentration of thiol groups, and the ferric reducing activity of plasma. Read More

    Down-regulated miR-448 relieves spinal cord ischemia/reperfusion injury by up-regulating SIRT1.
    Braz J Med Biol Res 2018 Mar 15;51(5):e7319. Epub 2018 Mar 15.
    Department of Human Morphology, Ningbo College of Health Sciences, Ningbo, Zhejiang, China.
    MicroRNAs play a crucial role in the progression of spinal cord ischemia/reperfusion injury (SCII). The role of miR-448 and SIRT1 in SCII was investigated in this study, to provide further insights into prevention and improvement of this disorder. In this study, expressions of miR-448 and SIRT1 protein were determined by qRT-PCR and western blot, respectively. Read More

    Thyrotropin and free thyroxine levels and coronary artery disease: cross-sectional analysis of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).
    Braz J Med Biol Res 2018 Mar 15;51(5):e7196. Epub 2018 Mar 15.
    Centro de Pesquisa Clínica, Hospital Universitário, Universidade de São Paulo, São Paulo, SP, Brasil.
    Data on the association between subclinical thyroid dysfunction and coronary artery disease (CAD) is scarce. We aimed to analyze the association between thyroid function and CAD using baseline data from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). We included subjects with normal thyroid function (0. Read More

    Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system.
    Braz J Med Biol Res 2018 Mar 15;51(5):e7183. Epub 2018 Mar 15.
    School of Medicine, Zhejiang University, Hangzhou, China.
    Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. Read More

    Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model.
    Braz J Med Biol Res 2018 Mar 15;51(5):e7132. Epub 2018 Mar 15.
    Laboratório de Cirurgia Experimental Fetal e Neonatal "Michael Harrison" Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
    Gastroschisis (GS) is an abdominal wall defect that results in histological and morphological changes leading to intestinal motility perturbation and impaired absorption of nutrients. Due to its anti-inflammatory, antioxidant, and neuroprotective effects, cannabidiol (CBD) has been used as a therapeutic agent in many diseases. Our aim was to test the effect of maternal CBD in the intestine of an experimental model of GS. Read More

    Threats to bioethical principles in medical practice in Brazil: new medical ethics code period.
    Braz J Med Biol Res 2018 Mar 15;51(5):e6988. Epub 2018 Mar 15.
    Faculdade de Medicina, Universidade do Porto, Porto, Portugal.
    We aimed to outline the profile of medical professionals in Brazil who have violated the deontological norms set forth in the ethics code of the profession, and whose cases were judged by the higher tribunal for medical ethics between 2010 and 2016. This survey was conducted using a database formed from professional ethics cases extracted from the plenary of the medical ethics tribunal of the Federal Council of Medicine. These were disciplinary ethics cases that were judged at appeal level between 2010 and 2016. Read More

    2-Methyl 2-butanol suppresses human retinoblastoma cells through cell cycle arrest and autophagy.
    Braz J Med Biol Res 2018 Mar 15;51(5):e6889. Epub 2018 Mar 15.
    Department of Fundus, Wuhan Aier Eye Hospital, Wuhan, Hubei Province, China.
    2-Methyl-2-butanol (MBT) is a chemical compound from the group of alcohols more specifically pentanols, which has shown an excellent anti-cancer activity in our previous study. However, its mechanism of action remains unclear. The present study was designed to investigate the anti-cancer effect of MBT on human retinoblastoma cells. Read More

    Salvinorin A preserves cerebral pial artery autoregulation after forebrain ischemia via the PI3K/AKT/cGMP pathway.
    Braz J Med Biol Res 2018 Mar 15;51(5):e6714. Epub 2018 Mar 15.
    Department of Anesthesiology, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
    This study aimed to investigate the protective effect of salvinorin A on the cerebral pial artery after forebrain ischemia and explore related mechanisms. Thirty Sprague-Dawley rats received forebrain ischemia for 10 min. The dilation responses of the cerebral pial artery to hypercapnia and hypotension were assessed in rats before and 1 h after ischemia. Read More

    Nitric oxide modulates ATP-evoked currents in mouse Leydig cells.
    Braz J Med Biol Res 2018 Mar 15;51(5):e6693. Epub 2018 Mar 15.
    Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
    Testosterone synthesis within Leydig cells is a calcium-dependent process. Intracellular calcium levels are regulated by different processes including ATP-activated P2X purinergic receptors, T-type Ca2+ channels modulated by the luteinizing hormone, and intracellular calcium storages recruited by a calcium-induced calcium release mechanism. On the other hand, nitric oxide (NO) is reported to have an inhibitory role in testosterone production. Read More

    Heterozygous HTRA1 missense mutation in CADASIL-like family disease.
    Braz J Med Biol Res 2018 Mar 15;51(5):e6632. Epub 2018 Mar 15.
    Department of Neurology, the First Hospital of Shanxi Medical University, Taiyuan, China.
    The aim of this study was to find related pathogenic genes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy in (CADASIL)-like patients. The direct sequencing and high-throughput multiplex polymerase chain reaction (PCR) was performed to screen for related genes. The clinical and imaging data of a CADASIL-like patient (the pro-band) and his family members were collected. Read More

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