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    6676 results match your criteria Brazilian Journal of Medical and Biological Research [Journal]

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    Association between polymorphisms in the APOB gene and hyperlipidemia in the Chinese Yugur population.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6613. Epub 2017 Sep 12.
    The Institute of Minority Physique and Health, Medical College of Northwest University for Nationalities, Lanzhou, China.
    We investigated the influence of apolipoprotein B gene (APOB) variants on the risk of hyperlipidemia (HL) in 631 middle-aged and elderly members of the Chinese Yugur population (HL, n=336; normolipidemia, n=295). APOB polymorphisms were identified using mass spectrometry, and five single nucleotide polymorphisms (rs1042034, rs2163204, rs512535, rs676210, and rs679899) and serum lipids were further analyzed. rs1042034 and rs676210 were significantly associated with HL (P<0. Read More

    Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6527. Epub 2017 Sep 12.
    Department of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
    Immunological mechanisms have been proposed to underlie the pathogenesis of recurrent spontaneous abortion (RSA). Vitamin D has a potent immunomodulatory effect, which may affect pregnancy outcome. The objective of this study was to investigate 25-hydroxyvitamin D [25(OH) D] concentration and vitamin D receptor (VDR) expression in the decidual tissues of RSA patients. Read More

    Synthesis and evaluation of coumarin derivatives against human lung cancer cell lines.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6455. Epub 2017 Sep 12.
    Department of Clinical Laboratory, Chongqing the Seventh People's Hospital, Chongqing, China.
    Series of novel coumarin derivatives [I (a-d) and II (a-d)] were successfully synthesized and their structures were determined based on infrared 1H-nuclear magnetic resonance (NMR), HRMS, and single crystal X-ray crystallography. Additionally, the new synthesized compounds were evaluated to identify the molecular characteristics that contribute to their cytotoxicity, which was tested against SK-LU-1, SPC-A-1 and 95D human lung cancer cell lines, using the MTT assay. The results of this study showed that compounds Ic, Id, IIc, and IId exhibited an efficient percentage of inhibition of cell proliferation. Read More

    Bradykinin, insulin, and glycemia responses to exercise performed above and below lactate threshold in individuals with type 2 diabetes.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6400. Epub 2017 Sep 12.
    Programa de Pós-graduação Stricto Sensu em Educação Física, Universidade Católica de Brasília, Brasília, DF, Brasil.
    The aim of this study was to analyze the acute responses of bradykinin, insulin, and glycemia to exercise performed above and below lactate threshold (LT) in individuals with type 2 diabetes mellitus (T2D). Eleven participants with a diagnosis of T2D randomly underwent three experimental sessions 72 h apart: 1) 20 min of exercise performed at 120% of LT (120%LT), 2) 20 min of exercise performed at 80% of LT (80%LT), and 3) 20 min of control session. Blood glucose was analyzed before, during, and at 45 min post-exercise. Read More

    Down-regulation of single-stranded DNA-binding protein 1 expression induced by HCMV infection promotes lipid accumulation in cells.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6389. Epub 2017 Sep 12.
    Department of Cardiology, Tianjin Chest Hospital, Tianjin Medical University, Tianjin, China.
    The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybridization and the expression levels of SSBP1 mRNA and protein after HCMV infection by real-time PCR and western blot. After verification of successful infection by indirect immunofluorescent staining and RT-PCR, we found a differential expression of lipid metabolism-related genes including LDLR, SCARB, CETP, HMGCR, ApoB and LPL induced by HCMV infection. Read More

    Tyrosine phosphorylation of Kv1.5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6237. Epub 2017 Sep 12.
    Department of Neonatology, the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.
    Intrauterine growth retardation (IUGR) is associated with the development of adult-onset diseases, including pulmonary hypertension. However, the underlying mechanism of the early nutritional insult that results in pulmonary vascular dysfunction later in life is not fully understood. Here, we investigated the role of tyrosine phosphorylation of voltage-gated potassium channel 1. Read More

    Molecular analysis of CIB4 gene and protein in Kermani sheep.
    Braz J Med Biol Res 2017 Sep 12;50(11):e6177. Epub 2017 Sep 12.
    Animal Science Department, Shahid Bahonar University of Kerman, Kerman, Iran.
    The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Read More

    Xuezhikang reduced arterial stiffness in patients with essential hypertension: a preliminary study.
    Braz J Med Biol Res 2017 Aug 31;50(10):e6363. Epub 2017 Aug 31.
    Department of Geriatric Cardiology, Chinese People's Liberation Army General Hospital, Beijing, China.
    This study aimed to test the effects of xuezhikang, a cholestin extract that contains statin-like components, on arterial stiffness in patients with essential hypertension. One hundred hypertensive patients from the Chinese PLA General Hospital were randomly allocated to receive xuezhikang (1200 mg/day, orally) or placebo (same capsules containing only pharmaceutical excipients). Physical examination outcomes, lipid profile, high sensitivity C-reactive protein (hs-CRP) levels, matrix metalloproteinases-9 (MMP-9) levels, and arterial outcomes, including stiffness parameter (β), pressure-strain elasticity modulus (Ep), arterial compliance (AC), augmentation index (AI), and one-point pulse wave velocity (PWVβ) were obtained at baseline and after 6 months of the intervention. Read More

    Evaluation of the toxicity and hypoglycemic effect of the aqueous extracts of Cnidoscolus quercifolius Pohl.
    Braz J Med Biol Res 2017 Aug 31;50(10):e6361. Epub 2017 Aug 31.
    Laboratório de Biotecnologia e Biologia Molecular, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
    Diabetes mellitus is one of the most common chronic degenerative diseases, and it is estimated to increase worldwide to around 415 million and to impact 642 million in 2040. Research shows that some plants are sources of bioactive compounds against diabetes. Thus, the objective of this work was to evaluate the oral toxicity and the hypoglycemic effect of the aqueous extract of the leaves of Cnidoscolus quercifolius Pohl. Read More

    Association between ADAM metallopeptidase domain 33 gene polymorphism and risk of childhood asthma: a meta-analysis.
    Braz J Med Biol Res 2017 Aug 31;50(10):e6148. Epub 2017 Aug 31.
    Department of Pediatrics, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
    This study aimed to investigate the association between ADAM metallopeptidase domain 33 (ADAM33) gene polymorphisms and the risk of childhood asthma. The relevant studies about the relationship between ADAM33 gene polymorphisms and childhood asthma were searched from electronic databases and the deadline of retrieval was May 2016. The single nucleotide polymorphisms (SNPs) of ADAM33 (rs511898, rs2280092, rs3918396, rs528557, rs2853209, rs44707, rs2280091 and rs2280089) were analyzed based on several models including the allele, codominant, recessive and dominant models. Read More

    Silencing of augmenter of liver regeneration inhibited cell proliferation and triggered apoptosis in U266 human multiple myeloma cells.
    Braz J Med Biol Res 2017 Aug 31;50(10):e6139. Epub 2017 Aug 31.
    Department of Hematology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
    Augmenter of liver regeneration (ALR) is a thermostable cytokine that was originally identified to promote the growth of hepatocytes. This study was conducted to explore the expression and function of ALR in multiple myeloma (MM), a common hematologic malignancy. Real-time PCR and western blot analysis were performed to detect the expression of ALR in U266 human MM cells and healthy peripheral blood mononuclear cells (PBMCs). Read More

    Dipyrone in association with atropine inhibits the effect on gastric emptying induced by hypoglycemia in rats.
    Braz J Med Biol Res 2017 Aug 31;50(10):e5948. Epub 2017 Aug 31.
    Departamento de Bioquímica e Biologia Tecidual, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
    Atropine (AT) and dipyrone (Dp) induce a delay of gastric emptying (GE) of liquids in rats by inhibiting muscarinic receptors and activating β2-adrenergic receptors, respectively. The objective of the present study was to determine the effects of pretreatment with AT and Dp, given alone or in combination, on the effect of hypoglycemia in the liquid GE in rats. Male Wistar adult rats (280-310 g) were pretreated intravenously with AT, Dp, AT plus Dp or their vehicle and then treated 30 min later with iv insulin or its vehicle (n=8-10 animals/group). Read More

    Application of data mining techniques to explore predictors of upper urinary tract damage in patients with neurogenic bladder.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6638. Epub 2017 Aug 17.
    Shenzhen Hospital, Southern Medical University, Shenzhen, China.
    This study proposed a decision tree model to screen upper urinary tract damage (UUTD) for patients with neurogenic bladder (NGB). Thirty-four NGB patients with UUTD were recruited in the case group, while 78 without UUTD were included in the control group. A decision tree method, classification and regression tree (CART), was then applied to develop the model in which UUTD was used as a dependent variable and history of urinary tract infections, bladder management, conservative treatment, and urodynamic findings were used as independent variables. Read More

    Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6586. Epub 2017 Aug 17.
    State Key Laboratory of Pharmaceutical Biotechnology, NJU-NJFU Joint Institute of Plant Molecular Biology, Nanjing University, Nanjing, China.
    Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), particularly in infants and children below 4 years of age. Shikonin is a bioactive compound with anti-inflammatory, antiviral, and antibacterial activities derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. This study aimed to examine the antiviral activity of PMM-034, a shikonin ester derivative, against EV71 in rhabdomyosarcoma (RD) cells. Read More

    Potential benefit of (-)-epigallocatechin-3-gallate for macrovascular complications in diabetes.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6511. Epub 2017 Aug 17.
    Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China.
    Vascular problems are the most common complications in diabetes. Substantial evidence from epidemiological and pathophysiological studies show that hyperglycemia is a major risk factor for macrovascular complications in patients with diabetes. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, is known to exert a variety of cardiovascular beneficial effects. Read More

    Is endobronchial ultrasound-guided transbronchial needle aspiration with a stylet necessary for lymph node screening in lung cancer patients?
    Braz J Med Biol Res 2017 Aug 17;50(10):e6372. Epub 2017 Aug 17.
    Department of Respiratory Medicine, Tai Zhou Hospital of Zhejiang Province, LinHai, Zhejiang Province, China.
    During endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), a needle is commonly used with a stylet, although recently the stylet has been omitted. This prospective study aimed to compare the quality of specimens obtained by EBUS-TBNA performed with and without a stylet. Between November 2013 and November 2014, 131 patients with lung cancer underwent EBUS-TBNA, with a total of 148 mediastinal or hilar lymph nodes sampled both with and without an inner-stylet, yielding 296 cytological specimens. Read More

    Proliferation and osteogenic activity of fibroblasts induced with fibronectin.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6272. Epub 2017 Aug 17.
    Tianjin Hospital, Tianjin, China.
    The aim of this study was to determine the proliferation and osteogenic activity of fibroblasts induced with fibronectin and their possible dose-dependent relationship. The fibroblasts obtained by tissue explants adherent method were induced with fibronectin at different concentrations of 0, 10, 20, 40, 60, and 80 μg/mL for 14 days. The 3H-thymidine and 3H-proline incorporation test was used to evaluate the synthesis of DNA and collagen by fibroblasts, respectively. Read More

    Methotrexate associated to lipid core nanoparticles improves cardiac allograft vasculopathy and the inflammatory profile in a rabbit heart graft model.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6225. Epub 2017 Aug 17.
    Instituto do Coração, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
    Coronary allograft vasculopathy is an inflammatory-proliferative process that compromises the long-term success of heart transplantation and has no effective treatment. A lipid nanoemulsion (LDE) can carry chemotherapeutic agents in the circulation and concentrates them in the heart graft. The aim of the study was to investigate the effects of methotrexate (MTX) associated to LDE. Read More

    Antidepressant effects of Kai-Xin-San in fluoxetine-resistant depression rats.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6161. Epub 2017 Aug 17.
    Department of Clinical Pharmacology, General Hospital of Chinese People's Liberation Army, Beijing, China.
    This study aimed to investigate the antidepressant effect and the mechanism of action of Kai-Xin-San (KXS) in fluoxetine-resistant depressive (FRD) rats. Two hundred male Wistar rats weighing 200±10 g were exposed to chronic and unpredictable mild stresses (CUMS) for 4 weeks and given fluoxetine treatment simultaneously. The rats that did not show significant improvement in behavioral indexes were chosen as the FRD model rats. Read More

    Salinomycin enhances doxorubicin sensitivity through reversing the epithelial-mesenchymal transition of cholangiocarcinoma cells by regulating ARK5.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6147. Epub 2017 Aug 17.
    Department of General Surgery, Qingdao Clinic Medical College, Nanjing Medical University, Qingdao, China.
    Chemotherapy response rates in patients with cholangiocarcinoma remain low, primarily due to the development of drug resistance. Epithelial-mesenchymal transition (EMT) of cancer cells is widely accepted to be important for metastasis and progression, but it has also been linked to the development of chemoresistance. Salinomycin (an antibiotic) has shown some potential as a chemotherapeutic agent as it selectively kills cancer stem cells, and has been hypothesized to block the EMT process. Read More

    Meta-analysis of the association between PADI4 -92C/G polymorphism and rheumatoid arthritis in the Chinese population.
    Braz J Med Biol Res 2017 Aug 17;50(10):e6115. Epub 2017 Aug 17.
    Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
    Many studies have evaluated the correlation between peptidylarginine deiminase 4 (PADI4) -92C/G polymorphism and rheumatoid arthritis (RA), but the results remain inconclusive. Therefore, we performed a meta-analysis in the Chinese population to provide comprehensive data on the association between PADI4 -92C/G polymorphism and RA. Eligible studies published before May 2016 were identified in PubMed and Chinese databases. Read More

    Comparative efficacy and safety of contact force-sensing catheter and second-generation cryoballoon ablation for atrial fibrillation: a meta-analysis.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6409. Epub 2017 Aug 7.
    Pacing and Electrophysiological Department, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
    This meta-analysis compared the efficacy and safety of the contact force (CF)-sensing catheter and second-generation cryoballoon (CB) ablation for treating atrial fibrillation (AF). Six controlled clinical trials comparing ablation for AF using a CF-sensing catheter or second-generation CB were identified from PubMed, EMBASE, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure. The procedure duration was significantly lower in the CB group compared with that in the CF group [mean difference (MD)=29. Read More

    Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6393. Epub 2017 Aug 7.
    Departamento de Fisioterapia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
    Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Read More

    Noninvasive intracranial pressure monitoring for HIV-associated cryptococcal meningitis.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6392. Epub 2017 Aug 7.
    Braincare Health Technology, São Carlos, SP, Brasil.
    Mortality and adverse neurologic sequelae from HIV-associated cryptococcal meningitis (HIV-CM) remains high due to raised intracranial pressure (ICP) complications. Cerebrospinal fluid (CSF) high opening pressure occurs in more than 50% of HIV-CM patients. Repeated lumbar puncture with CSF drainage and external lumbar drainage might be required in the management of these patients. Read More

    Effect of strontium ranelate on pain behavior in an experimental model of osteoarthritis.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6314. Epub 2017 Aug 7.
    Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, São Luís, MA, Brasil.
    Strontium ranelate (SrRan) is a drug usually prescribed to treat osteoporosis, with proven effects of decreasing the risk of fractures and an indication of reducing the progression of osteoarthritis (OA). This study aimed to investigate the effects of SrRan as either a prophylactic or a treatment drug, using an OA rat model to assess pain behavior. A monoiodoacetate (MIA)-induced knee joint OA model in Wistar rats was used. Read More

    Association between Toll-like receptor 4 Asp299Gly polymorphism and coronary heart disease susceptibility.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6306. Epub 2017 Aug 7.
    Department of Cardiology, Huashan Hospital, Fudan University, Shanghai, China.
    Published data on the association between Toll-like receptor 4 (TLR4) Asp299Gly polymorphism and coronary heart disease (CHD) susceptibility are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. English-language studies were identified by searching PubMed and Embase databases (up to November 2016). Read More

    Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA network.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6297. Epub 2017 Aug 7.
    Department of Pulmonary Disease, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Jilin Province, China.
    In our study, we aimed to reveal potential long non-coding RNAs (lncRNA) biomarkers in lung adenocarcinoma (LAD) using lncRNA-mediated competing endogenous RNAs (ceRNAs) network (LMCN). Competing lncRNA-mRNA interactions were identified using the hypergeometric test. Co-expression analysis for the competing lncRNA-mRNA interactions was implemented, and relying on the weight value >0. Read More

    Cerebrospinal fluid-contacting nucleus mediates nociception via release of fractalkine.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6275. Epub 2017 Aug 7.
    Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
    Increasing evidence suggests that the cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) mediates the transduction and regulation of pain signals. However, the precise molecular mechanisms remain unclear. Studies show that release of fractalkine (FKN) from neurons plays a critical role in nerve injury-related pain. Read More

    Cordyceps sinensis promotes immune regulation and enhances bacteriostatic activity of PA-824 via IL-10 in Mycobacterium tuberculosis disease.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6188. Epub 2017 Aug 7.
    Department of Respiratory Medicine, East Medical District of Linyi People's Hospital, Linyi, Shandong, China.
    PA-824 is a novel bicyclic nitroimidazole anti-tuberculosis (TB) drug. Cordyceps sinensis (Berk.) Sacc. Read More

    Resistance training attenuates salt overload-induced cardiac remodeling and diastolic dysfunction in normotensive rats.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6146. Epub 2017 Aug 7.
    Laboratório de Fisiologia Molecular, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
    Elevated salt intake induces changes in the extracellular matrix collagen, leading to myocardial stiffness and impaired relaxation. Resistance training (RT) has been used as a remarkably successful strategy in the treatment of heart disease. Therefore, the aim of this study was to investigate the effects of RT on preventing pathological adaptation of the left ventricle (LV) induced by salt overload. Read More

    Relationship between uric acid levels and risk of chronic kidney disease in a retrospective cohort of Brazilian workers.
    Braz J Med Biol Res 2017 Aug 7;50(9):e6048. Epub 2017 Aug 7.
    Divisão de Nefrologia, Departamento de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brasil.
    Uric acid (UA) levels are increased in patients with kidney dysfunction. We analyzed the association between asymptomatic hyperuricemia and new-onset chronic kidney disease (CKD). A retrospective cohort study was designed to collect data from employees of an energy generation and distribution company in the city of Rio de Janeiro, Brazil, who had undergone the company's annual medical checkup from 2008 to 2014. Read More

    Possible mechanisms involved in the vasorelaxant effect produced by clobenzorex in aortic segments of rats.
    Braz J Med Biol Res 2017 Aug 7;50(9):e5765. Epub 2017 Aug 7.
    Department of Physiology and Cellular Development, National Institute of Perinatology, Mexico City, Mexico.
    Clobenzorex is a metabolic precursor of amphetamine indicated for the treatment of obesity. Amphetamines have been involved with cardiovascular side effects such as hypertension and pulmonary arterial hypertension. The aim of the present study was to investigate whether the direct application of 10-9-10-5 M clobenzorex on isolated phenylephrine-precontracted rat aortic rings produces vascular effects, and if so, what mechanisms may be involved. Read More

    Development of VEGF-loaded PLGA matrices in association with mesenchymal stem cells for tissue engineering.
    Braz J Med Biol Res 2017 Aug 7;50(9):e5648. Epub 2017 Aug 7.
    Laboratório de Hematologia e Células Tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
    The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured. Read More

    Impact of extracellular alkalinization on the survival of human CD24-/CD44+ breast cancer stem cells associated with cellular metabolic shifts.
    Braz J Med Biol Res 2017 Jul 20;50(8):e6538. Epub 2017 Jul 20.
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
    Cancer stem cells reside in a distinct region within the tumor microenvironment that it is believed to play a fundamental role in regulating stemness, proliferation, survival, and metabolism of cancer cells. This study aimed to analyze the effect of extracellular alkalinization on metabolism and survival of human CD24-/CD44+ breast cancer stem cells (BCSCs). BCSCs were cultured in alkalinized DMEM-F12 and incubated at 37°C, 5% CO2, and 20% O2 for 30 min, 6, 24, and 48 h. Read More

    Relationship between ventilation heterogeneity and exercise intolerance in adults with sickle cell anemia.
    Braz J Med Biol Res 2017 Jul 20;50(8):e6512. Epub 2017 Jul 20.
    Programa de Pós-Graduação em Ciências Médicas, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
    Sickle cell anemia (SCA) causes dysfunction of multiple organs, with pulmonary involvement as a major cause of mortality. Recently, there has been growing interest in the nitrogen single-breath washout (N2SBW) test, which is able to detect ventilation heterogeneity and small airway disease when the results of other pulmonary function tests (PFTs) are still normal. Thus, the objectives of the present study were to assess the heterogeneity in the ventilation distribution in adults with SCA and to determine the association between the ventilation distribution and the clinical, cardiovascular, and radiological findings. Read More

    Interleukin-27 augments the inhibitory effects of sorafenib on bladder cancer cells.
    Braz J Med Biol Res 2017 Jul 20;50(8):e6207. Epub 2017 Jul 20.
    Department of Gastrointestinal Surgery, Binzhou Medical University Hospital, Binzhou, China.
    Both sorafenib and interleukin-27 (IL-27) are antineoplastic drugs. This study aimed to investigate the synergistic effect of these two drugs on bladder cancer cells. HTB-9 and T24 cells were stimulated with IL-27 (50 ng/mL), sorafenib (2 μM) or the synergistic action of these two drugs. Read More

    Ischemic preconditioning vs adenosine vs prostaglandin E1 for protection against liver ischemia/reperfusion injury.
    Braz J Med Biol Res 2017 Jul 20;50(8):e6185. Epub 2017 Jul 20.
    School of Medicine, University of Nis, Nis, Serbia.
    Ischemia/reperfusion injury is still a major cause of morbidity and mortality during liver surgery and transplantation. A variety of surgical and pharmacological therapeutic strategies have been investigated to minimize the effects of ischemia/reperfusion. The aim of our study was to analyze and compare preventive influences of ischemic preconditioning, adenosine and prostaglandin E1 in the experimental model of hepatic ischemia/reperfusion injury. Read More

    Tangeretin has anti-asthmatic effects via regulating PI3K and Notch signaling and modulating Th1/Th2/Th17 cytokine balance in neonatal asthmatic mice.
    Braz J Med Biol Res 2017 Jul 20;50(8):e5991. Epub 2017 Jul 20.
    Children's Medical Center, Qilu Hospital of Shandong University, Jinan, Shandong, China.
    Asthma is a chronic allergic disease characterized by airway inflammation, airway hyper-responsiveness (AHR), and mucus hypersecretion. T-lymphocytes are involved in the pathogenesis of asthma, mediating airway inflammatory reactions by secreting cytokines. The phosphoinositide 3-kinase (PI3K) and Notch signaling pathways are associated with T cell signaling, proliferation, and differentiation, and are important in the progression of asthma. Read More

    MicroRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer.
    Braz J Med Biol Res 2017 Jul 20;50(8):e5891. Epub 2017 Jul 20.
    Department of Pathology, Huaihe Hospital of Henan University, Kaifeng, China.
    This study aimed to investigate the function and mechanism of microRNA-143 (miR-143) in the occurrence and development of breast cancer (BC). A total of 30 BC tissues, 30 corresponding noncancerous tissues, and 10 normal control (NC) breast tissues were obtained to detect the levels of miR-143, extracellular signal-regulated kinase 5 (ERK5) and mitogen-activated protein 3 kinase 7 (MAP3K7) using RT-qPCR, western blotting or immunohistochemistry. The correlation of miR-143 with ERK5 or MAP3K7 was evaluated using Pearson correlation analysis. Read More

    Patients with Duchenne and Becker muscular dystrophies are not more asymmetrical than healthy controls on timed performance of upper limb tasks.
    Braz J Med Biol Res 2017 Jul 24;50(8):e6031. Epub 2017 Jul 24.
    Departamento de Fonoaudiologia, Fisioterapia e Terapia Ocupacional, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
    This study aimed to investigate possible asymmetries and relationships between performance of dominant and non-dominant upper limbs (UL) in patients with Duchenne and Becker muscular dystrophies (DMD/BMD), to compare UL performance of patients and healthy subjects and to investigate the relationship between timed performance of UL and age, motor function and muscle strength in DMD/BMD patients. Sixteen patients with DMD and 3 with BMD were evaluated with Jebsen-Taylor Test (timed performance), Vignos scale and Dimension 3 of Motor Function Measure (motor function), and Medical Research Council scale (muscle strength) on a single session. ANOVA showed no asymmetry between dominant and non-dominant UL, except in the writing subtest, in patients and in healthy controls. Read More

    Excavation of attractor modules for nasopharyngeal carcinoma via integrating systemic module inference with attract method.
    Braz J Med Biol Res 2017 Jul 10;50(8):e6416. Epub 2017 Jul 10.
    Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China.
    The molecular mechanism of nasopharyngeal carcinoma (NPC) is poorly understood and effective therapeutic approaches are needed. This research aimed to excavate the attractor modules involved in the progression of NPC and provide further understanding of the underlying mechanism of NPC. Based on the gene expression data of NPC, two specific protein-protein interaction networks for NPC and control conditions were re-weighted using Pearson correlation coefficient. Read More

    Application of endoscopy to treat mandibular keratocystic odontogenic tumors.
    Braz J Med Biol Res 2017 Jul 10;50(8):e6209. Epub 2017 Jul 10.
    Department of Oral and Maxillofacial Surgery, General Hospital of Xinjiang Military Region, Urumqi, China.
    The aim of this study was to evaluate the feasibility of endoscopy to remove keratocystic odontogenic tumors (KCOTs) with virtual 3D mandibular images. Fifteen patients (mean age, 40.27±14. Read More

    Astragalus polysaccharides improve cardiomyopathy in STZ-induced diabetic mice and heterozygous (SOD2+/-) knockout mice.
    Braz J Med Biol Res 2017 Jul 10;50(8):e6204. Epub 2017 Jul 10.
    Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai, China.
    Oxidative stress plays an important role in the development of diabetic cardiomyopathy. In the present study, we determined whether the effect of astragalus polysaccharides (APS) on diabetic cardiomyopathy was associated with its impact on oxidative stress. Streptozotocin (STZ)-induced diabetic mice and heterozygous superoxide dismutase (SOD2+/-) knockout mice were administered APS. Read More

    Lipoprotein(a) as a predictor of poor collateral circulation in patients with chronic stable coronary heart disease.
    Braz J Med Biol Res 2017 Jul 10;50(8):e5979. Epub 2017 Jul 10.
    Department of Cardiology, Renmin Hospital, Wuhan University Hubei General Hospital, Wuhan, Hubei Province, China.
    As a mechanism compensating for obstructive coronary artery disease, coronary collateral circulation (CCC) has attracted cardiologists for a long time to explore its potential impact. In the present study, Chinese patients suffering from ≥95% coronary stenosis, as diagnosed by angiography, have been investigated for the correlation between CCC and lipoprotein(a) [Lp(a)] levels. A cohort of 654 patients was divided into four categories according to Rentrop grades 0, 1, 2, and 3. Read More

    Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro.
    Braz J Med Biol Res 2017 Jul 10;50(8):e5163. Epub 2017 Jul 10.
    Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brasil.
    Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. Read More

    Two novel Co(II) complexes with two different Schiff bases: inhibiting growth of human skin cancer cells.
    Braz J Med Biol Res 2017 Jul 3;50(7):e6390. Epub 2017 Jul 3.
    Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
    Using two flexible Schiff bases, H2L1 and H2L2, two new cobalt II (Co(II))-coordination compounds, namely, Py3CoL1 (1) and Py3CoL2 (2) (Py=pyridine, L1=3,5-ClC6H2(O)C=NC6H3(O)-4-NO2, L2=3,5-BrC6H2(O)C=NC6H3(O)-4-NO2) have been synthesized under solvothermal conditions. Single crystal X-ray structural analysis revealed that compounds 1 and 2 are both six-coordinate in a distorted octahedral geometry, and the 1D chain structure was formed by the π…π and C-H…O interactions or C-H…Cl interaction. The in vitro antitumor activities of 1, 2 and their corresponding organic ligands Py, L1, and L2 were studied and evaluated, in which three human skin cancer cell lines (A-431, HT-144 and SK-MEL-30) were used in the screening tests. Read More

    Identification of IgE and IgG1 specific antigens in Echinococcus granulosus cyst fluid.
    Braz J Med Biol Res 2017 Jul 3;50(7):e6071. Epub 2017 Jul 3.
    Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
    Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and identify the antigens of specific IgE and IgG1 (sIgE and sIgG1) in E. Read More

    Pain and distress outcomes in infants and children: a systematic review.
    Braz J Med Biol Res 2017 Jul 3;50(7):e5984. Epub 2017 Jul 3.
    Departamento de Neurociências e Ciências do Comportamento, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
    The aim of the present study was to systematically review the recent literature about pain and distress outcomes in children and critically analyze the methodological quality of the reports. The systematic review was based on the PRISMA statement and performed by selecting articles that are indexed in scientific databases. The methodological quality of reports was examined using STROBE statement, for observational studies, and CONSORT statement, for randomized controlled trials. Read More

    microRNA-142-3p inhibits apoptosis and inflammation induced by bleomycin through down-regulation of Cox-2 in MLE-12 cells.
    Braz J Med Biol Res 2017 Jul 3;50(7):e5974. Epub 2017 Jul 3.
    Department of Respiratory Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China.
    microRNA (miR)-142-3p is implicated in malignancy and has been identified as a biomarker for aggressive and recurrent lung adenocarcinomas. This study aimed to evaluate the inhibitory effect of miR-142-3p on apoptosis and inflammation induced by bleomycin in MLE-12 cells. MLE-12 cells were first transfected either with miR-142-3p mimic or miR-142-3p inhibitor and then the cells were exposed to 50 μg/mL of bleomycin. Read More

    Cantharidin suppressed breast cancer MDA-MB-231 cell growth and migration by inhibiting MAPK signaling pathway.
    Braz J Med Biol Res 2017 Jul 3;50(7):e5920. Epub 2017 Jul 3.
    Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
    As an active constituent of the beetle Mylabris used in traditional Chinese medicine, cantharidin is a potent and selective inhibitor of protein phosphatase 2A (PP2A) that plays a crucial role in cell cycle progression, apoptosis, and cell fate. The role and possible mechanisms exerted by cantharidin in cell growth and metastasis of breast cancer were investigated in this study. Cantharidin was found to inhibit cell viability and clonogenic potential in a time- and dose-dependent manner. Read More

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