1,892 results match your criteria Brazilian Journal Of Microbiology[Journal]


Rapid detection of Salmonella contamination in seafoods using multiplex PCR.

Braz J Microbiol 2019 Apr 21. Epub 2019 Apr 21.

ICAR-CIFRI, Barrackpore, Kolkata, 700120, India.

Effective monitoring of Salmonella contamination in seafood processing to conform the requirements of HACCP is a great challenge today. Such challenges can be effectively addressed, if the conventional detection methods are replaced with DNA-based molecular methods. Accordingly, it was aimed to develop a robust PCR protocol for specific detection of Salmonella spp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00072-8DOI Listing

Genome sequencing of Burkholderia contaminans LTEB11 reveals a lipolytic arsenal of biotechnological interest.

Braz J Microbiol 2019 Apr 18. Epub 2019 Apr 18.

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx. P. 19046 Centro Politécnico, Curitiba, Paraná, 81531-980, Brazil.

Burkholderia contaminans LTEB11 is a Gram-negative betaproteobacterium isolated as a contaminant of a culture in mineral medium supplemented with vegetable oil. Here, we report the genome sequence of B. contaminans LTEB11, identifying and analyzing the genes involved in its lipolytic machinery and in the production of other biotechnological products. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00076-4DOI Listing

Absence of influence of polymorphisms of the MBL2 gene in oral infections by HSV-1 in individuals with HIV.

Braz J Microbiol 2019 Apr 18. Epub 2019 Apr 18.

University Federal of Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.

Polymorphisms in the structural gene MBL-2 (mannose-binding lectin-2) may result in low MBL serum concentration, associated with greater susceptibility to infection. The study evaluated the effects of MBL-2 polymorphisms with the oral manifestations of the HSV in human immunodeficiency virus (HIV)-infected patients. An observational case-control study was carried out, with the sample comprising 64 HIV+ and 65 healthy individuals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00074-6DOI Listing

Spread of the serotypes and antimicrobial resistance in strains of Salmonella spp. isolated from broiler.

Braz J Microbiol 2019 Apr 18. Epub 2019 Apr 18.

Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Rua Ceará, s/n, Bloco 2D, Sala 43, Campus Umuarama, Uberlândia, MG, Brazil.

Serotypes and antimicrobial susceptibility of Salmonella's isolates from broiler production chain were determined. A total of 239 isolated strains from chicken, carcasses, breeding environments, and slaughter was analyzed by disk diffusion test, in the period of 2009 to 2010. For antibiotics with a high number of resistant strains, the minimal inhibitory concentration (MIC) was performed. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s42770-019-00054-w
Publisher Site
http://dx.doi.org/10.1007/s42770-019-00054-wDOI Listing
April 2019
2 Reads

Disseminated melanized fungal infection due to Cladosporium halotolerans in a dog coinfected with canine adenovirus-1 and canine parvovirus-2.

Braz J Microbiol 2019 Apr 17. Epub 2019 Apr 17.

Small Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

This report presents the pathologic findings associated with disseminated infection due to Cladosporium halotolerans in a dog that was simultaneously infected with canine adenovirus-1 (CAdV-1) and canine parvovirus-2 (CPV-2). A 12-year-old, mixed breed dog, with a clinical history of neurological manifestations was submitted for routine autopsy due to poor prognosis. The principal pathologic findings were mycotic necrotizing nephritis, hepatitis, and splenitis with embolic dissemination to the brain resulting in mycotic necrotizing meningoencephalitis, ventriculitis, choroid plexitis, and obstructive hydrocephalus associated with intralesional and intravascular septate pigmented fungi. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00082-6DOI Listing

Enhanced rhizoremediation of crude oil-contaminated mangrove swamp soil using two wetland plants (Phragmites australis and Eichhornia crassipes).

Braz J Microbiol 2019 Apr 16. Epub 2019 Apr 16.

Department of Microbiology, Delta State University, Abraka, Nigeria.

Comparative studies of enhanced rhizoremediation with biostimulation and bioaugmentation techniques in remediation of oil-contaminated mangrove environment were investigated. Contaminated soils at 7190 mg/kg of oil were subjected to the following treatments: soil (S), soil + oil (SO), soil + oil + fertilizer (NPK) (SOF), soil + oil + fertilizer + microorganisms (SOFM), soil + oil + fertilizer + microorganisms + solarization (SOFMS) (triplicates): two sets planted with P. australis, E. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00077-3DOI Listing

Hydrophobin HFBII-4 from Trichoderma asperellum induces antifungal resistance in poplar.

Braz J Microbiol 2019 Apr 13. Epub 2019 Apr 13.

School of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Herein, the class II hydrophobin gene HFBII-4 was cloned from the biocontrol agent Trichoderma asperellum ACCC30536 and recombinant rHFBII-4 was expressed in Pichia pastoris GS115. Treatment of Populus davidiana × P. alba var. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s42770-019-00083-5
Publisher Site
http://dx.doi.org/10.1007/s42770-019-00083-5DOI Listing
April 2019
2 Reads

Prevalence and characterization of Listeria monocytogenes isolated from pork meat and on inert surfaces.

Braz J Microbiol 2019 Apr 11. Epub 2019 Apr 11.

Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 sur, Col. Centro, 85000, Ciudad Obregón, Sonora, Mexico.

This study focuses on the prevalence of Listeria monocytogenes (Lm) in pork meat and on inert surfaces from slaughterhouses in Sonora, Mexico. A total of 21 Lm were obtained from 103 samples, giving a prevalence of 20.3%. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00073-7DOI Listing

Epidemiology and antimicrobial susceptibility profiles of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae and Escherichiacoli in China.

Braz J Microbiol 2019 Apr 10. Epub 2019 Apr 10.

Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun, Jilin Province, China.

Objects: The retrospective study aimed to determine the prevalence rate and antimicrobial susceptibility of extended-spectrum beta-lactamases (ESBLs)-producing Klebsiella pneumoniae and Escherichia coli in 2013-2017 at a single center in China.

Methods: Klebsiella pneumoniae and Escherichia coli data were collected from the microbiological laboratory. VITEK 2 compact system was used for the identification and antimicrobial susceptibility testing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00081-7DOI Listing

Molecular detection and phylogeny of bovine viral diarrhea virus 1 among cattle herds from Northeast, Southeast, and Midwest regions, Brazil.

Braz J Microbiol 2019 Mar 16. Epub 2019 Mar 16.

Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

We examined the circulating BVDV species and genotypes among cattle herds from Northeast, Southeast, and Midwest regions in Brazil. A total of 77 animals tested positive through standard PCR. Phylogenetic analyses revealed the presence of BVDV-1a, highlighting the need for better surveillance strategies to prevent BVDV spread in the country. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00064-8DOI Listing

Ability of endophytic fungi associated with Withania somnifera L. to control Fusarium Crown and Root Rot and to promote growth in tomato.

Braz J Microbiol 2019 Mar 15. Epub 2019 Mar 15.

UR13AGR09-Integrated Horticultural Production in the Tunisian Centre-East, Regional Center of Research on Horticulture and Organic Agriculture, University of Sousse, 4042, Chott-Mariem, Tunisia.

Fusarium crown and root rot (FCRR), caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is a soilborne tomato disease of increased importance worldwide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00062-wDOI Listing
March 2019
1 Read

Genetic identification of pestiviruses from beef cattle in Southern Brazil.

Braz J Microbiol 2019 Mar 15. Epub 2019 Mar 15.

Departamento de Medicina Veterinária Preventiva (DMVP), Setor de Virologia (SV), Universidade Federal de Santa Maria (UFSM), Avenida Roraima, 1000, prédio 63A, Centro de Eventos, Santa Maria, RS, 97105-900, Brazil.

Bovine pestiviruses, e.g., bovine viral diarrhea virus types 1 (BVDV-1 or Pestivirus A), BVDV-2 (Pestivirus B), and HoBi-like pestiviruses (HoBiPeV or Pestivirus H), have been shown to circulate in Brazilian cattle in varied proportions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00058-6DOI Listing

Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo.

Braz J Microbiol 2019 Mar 15. Epub 2019 Mar 15.

Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil.

Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00065-7DOI Listing
March 2019
4 Reads

The Perennial Use of the Green Fluorescent Protein Marker in a Live Vaccinia Virus Ankara Recombinant Platform Shows No Acute Adverse Effects in Mice.

Braz J Microbiol 2019 Mar 15. Epub 2019 Mar 15.

Laboratory of Basic and Applied Virology, Departmento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Recombinant virus vectors represent a promising strategy for vaccine research. Among available viral vectors, members of the Poxviridae family-especially the modified Vaccinia virus Ankara (MVA)-stand out as immunogenic and safe vaccine platforms. Because MVA usually does not produce plaques in cell culture, visible selection markers such as the green fluorescent protein (GFP) are frequently incorporated into the constructions in order to facilitate the recognition of recombinants. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00067-5DOI Listing
March 2019
1 Read

Correction to: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test.

Braz J Microbiol 2019 Mar 14. Epub 2019 Mar 14.

Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700 - Centro, Alfenas, MG, CEP 37130-001, Brazil.

In the article mentioned above an author's name was misspelled. The correct author name reads as follows: Leila Maria Lopes-Bezerra. We apologize for the inconvenience. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00070-wDOI Listing
March 2019
1 Read

Potential of oxygen and nitrogen reactive intermediates to disperse Listeria monocytogenes from biofilms.

Braz J Microbiol 2019 Mar 13. Epub 2019 Mar 13.

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Av. do Café s/n, Ribeirão Preto, São Paulo, 14040-903, Brazil.

Studying biofilm dispersal is important to prevent Listeria monocytogenes persistence in food processing plants and to avoid finished product contamination. Reactive oxygen and nitrogen intermediates (ROI and RNI, respectively) may trigger cell detachment from many bacterial species biofilms, but their roles in L. monocytogenes biofilms have not been fully investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00069-3DOI Listing

Detection of Enterobacterales resistant to polymyxins using Rapid Polymyxins NP test.

Braz J Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.

Two hundred isolates of Enterobacterales were tested by Rapid Polymyxins NP for the detection of polymyxin resistance and compared to the reference test broth microdilution (BMD). The sensitivity and specificity of the NP test were 98% and the results are faster than the BMD, decreasing from approximately 24 to 2 h. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00053-xDOI Listing

Physiological and molecular insights of bacteriocin production by Enterococcus hirae ST57ACC from Brazilian artisanal cheese.

Braz J Microbiol 2019 Mar 9. Epub 2019 Mar 9.

Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Viçosa, MG, 36570-900, Brazil.

The bacteriocinogenic Enterococcus hirae ST57ACC recently isolated from a Brazilian artisanal cheese was subjected here to additional analyses in order to evaluate its bacteriocin production and the potential influence of ABC transporter system in its expression. Besides these physiological and molecular aspects, the bacteriocin was evaluated for its cytotoxicity against HT-29. Differences in the inoculum size had no impact on the growth of E. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00068-4DOI Listing

Cryptococcus gattii VGII isolated from native forest and river in Northern Brazil.

Braz J Microbiol 2019 Mar 9. Epub 2019 Mar 9.

Laboratório de Micologia Médica, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Aleixo, Manaus, AM, 69060-001, Brazil.

Background: Cryptococcosis is a global invasive mycosis associated with significant morbidity and mortality. In the northern region of Brazil, this disease is caused by Cryptococcus neoformans genotype VNI and Cryptococcus gattii genotype VGII. However, few environmental studies have been conducted in this large tropical area. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00066-6DOI Listing
March 2019
1 Read

Genome sequence of Xanthomonas fuscans subsp. fuscans strain Xff49: a new isolate obtained from common beans in Southern Brazil.

Braz J Microbiol 2019 Mar 9. Epub 2019 Mar 9.

Laboratório de Bioinformática e Proteômica, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Capão do Leão, Brazil.

The genus Xanthomonas comprises Gram-negative bacteria, many of which are phytopathogens. Xanthomonas fuscans subsp. fuscans is one of the most devastating pathogens affecting the bean plant, resulting in the common bacterial blight of bean (CBB). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00050-0DOI Listing

Semisolid culture medium improves mycelial recovery of Agaricus subrufescens cryopreserved in cereal grains.

Braz J Microbiol 2019 Mar 8. Epub 2019 Mar 8.

Graduate Program of Biotechnology Applied to Agriculture, Molecular Biology Laboratory, Paranaense University, CP 224, Umuarama, PR, 87.502-210, Brazil.

This study aimed to evaluate the effects of the solid and semisolid culture medium on the mycelial viability of A. subrufescens after 5-year cryopreservation at - 70 °C. Mycelia were grown in three types of whole or ground grains, with or without 5% glycerol addition in the substrate and/or in a cryotube. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00063-9DOI Listing

Interaction between the production of ethanol and glycerol in fed-batch bioreactors.

Braz J Microbiol 2019 Mar 8. Epub 2019 Mar 8.

Biocontal - Tecnologia em Bioprocessos, Campinas, SP, Brazil.

During alcoholic fermentation, most of the substrates supplied to the yeasts are converted into ethanol and carbon dioxide generating energy for cell maintenance. However, some of these substrates end up being diverted to other metabolic pathways generating by-products reducing the yield in ethanol production. Glycerol is the most important by-product quantitatively, and its production during fermentation is associated to the production of ethanol. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s42770-019-00051-z
Publisher Site
http://dx.doi.org/10.1007/s42770-019-00051-zDOI Listing
March 2019
8 Reads

Heterologous expression and functional characterization of the ligand-binding domain of oxysterol-binding protein from Aspergillus oryzae.

Braz J Microbiol 2019 Mar 8. Epub 2019 Mar 8.

Jiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang, 330013, China.

Oxysterol-binding proteins (OSBPs) comprise a family of sterol-binding proteins. In this study, we focused on AoOSBP1, one of the five OSBP proteins identified from the industrial fungus Aspergillus oryzae. The temporal expression pattern analysis showed that the expression of AoOSBP1, in both gene and protein levels, was stably expressed throughout the developmental stages, while was upregulated during the accelerated growth stage. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s42770-019-00060-y
Publisher Site
http://dx.doi.org/10.1007/s42770-019-00060-yDOI Listing
March 2019
5 Reads

Outbreak of contagious ecthyma caused by Orf virus (Parapoxvirus ovis) in a vaccinated sheep flock in Uruguay.

Braz J Microbiol 2019 Mar 5. Epub 2019 Mar 5.

Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50 Km 11, La Estanzuela, Colonia, Uruguay.

Orf virus (ORFV) causes contagious ecthyma (CE), a highly transmissible, zoonotic disease of small ruminants. CE most commonly affects lambs and unvaccinated sheep. This work reports epidemiologic, clinicopathologic, and virologic findings in a CE outbreak in a vaccinated sheep flock in Uruguay and failure to detect ORFV in a commercial vaccine. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00057-7DOI Listing
March 2019
1 Read

Enterobacter sp. strain Fs-11 adapted to diverse ecological conditions and promoted sunflower achene yield, nutrient uptake, and oil contents.

Braz J Microbiol 2019 Mar 4. Epub 2019 Mar 4.

Microbial Physiology Laboratory, National Institute for Biotechnology & Genetic Engineering (NIBGE), Faisalabad, Pakistan.

Plant growth-promoting rhizobacteria are under extensive investigation to supplement the chemical fertilizers due to cost-effective and eco-friendly nature. However, their consistency in heterogeneous soil and diverse ecological settings is unclear. The current study presents in vitro and field evaluation of pre-characterized PGPR strain Enterobacter sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00061-xDOI Listing
March 2019
1 Read

Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies.

Braz J Microbiol 2019 Mar 2. Epub 2019 Mar 2.

Institute of Chemistry, Department of Biochemistry, Laboratory of Microbial Biotechnology, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149 - CT - Bloco A - Sala 549-1 - Cidade Universitária - Ilha do Fundão, Rio de Janeiro, RJ, 21941-909, Brazil.

The effect of gene dosage on the production of Candida antarctica lipase B (CalB) in the methylotrophic yeast Komagataella phaffii, at high densities in a simple medium containing crude glycerin as the sole carbon source, is described. The use of crude glycerin, the main by-product of biodiesel production from vegetable oils, will reduce the production cost of the bioprocess. Two K. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00056-8DOI Listing

Fermentative capabilities of native yeast strains grown on juices from different Agave species used for tequila and mezcal production.

Braz J Microbiol 2019 Mar 2. Epub 2019 Mar 2.

Biotecnología Industrial, CIATEJ, Camino Arenero 1227, El Bajío, 45019, Zapopan, Jalisco, Mexico.

The Asparagaceae family is endemic from America, being the Agave genus the most important. The Agave species possess economic relevance and are use as raw material to produce several distilled alcoholic beverages, as bacanora, tequila, and mezcal. The fermentation process has been carry out either spontaneously or by adding a selected yeast strain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00049-7DOI Listing
March 2019
3 Reads

Fermentation of yam (Dioscorea spp. L.) by indigenous phytase-producing lactic acid bacteria strains.

Braz J Microbiol 2019 Feb 26. Epub 2019 Feb 26.

Department of Biology, Federal University of Lavras, Campus Universitário, 3037, Lavras, MG, 37200-000, Brazil.

The use of lactic bacteria in the development of functional foods has increased in recent years. In addition to their probiotic characteristics, they can ferment a variety of substrates, such as cereals, roots, and tubers. Phytase producer lactic acid bacteria strains and their behavior during the fermentation process of yam-based food were studied. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00059-5DOI Listing
February 2019

Genetic polymorphism in association with susceptibility to tuberculosis: a study in a Pakistani population.

Braz J Microbiol 2019 Feb 25. Epub 2019 Feb 25.

Government College University Faisalabad, Faisalabad, Pakistan.

Tuberculosis is becoming a global issue with raising occurrences; particularly in developing countries, the situation is alarming. Besides environmental factors, host genetic factors are vital in disease development. A demographical and genotypic analysis in relation to tuberculosis commencement is conducted in a Pakistani population, and genotypic frequency of EBI3 (rs4740) was analyzed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00048-8DOI Listing
February 2019
3 Reads

Salmonella isolates from urine cultures: serotypes and antimicrobial resistance in hospital settings.

Braz J Microbiol 2019 Feb 22. Epub 2019 Feb 22.

Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada à Saúde, Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, Brazil.

This study investigated the serotypes and antimicrobial resistance of Salmonella isolates in urine cultures from 38 hospitalized patients. Nine serotypes were detected, and a large proportion was Typhimurium and Enteritidis. The strains presented resistance to 11 different antibiotics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00052-yDOI Listing
February 2019

Multiplex-PCR for diagnosis of bacterial meningitis.

Braz J Microbiol 2019 Feb 22. Epub 2019 Feb 22.

Faculdade de Ciências Farmacêuticas, Department of Clinical Chemistry and Toxicology, Universidade de São Paulo (USP), São Paulo, Brazil.

Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00055-9DOI Listing
February 2019
1 Read

Classification of the inoculant strain of cowpea UFLA03-84 and of other strains from soils of the Amazon region as Bradyrhizobium viridifuturi (symbiovar tropici).

Braz J Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, 37200-000, Brazil.

Cowpea (Vigna unguiculata L.) is a legume species that considerably benefits from inoculation with nitrogen fixing bacteria of the genus Bradyrhizobium. One of the strains recommended for inoculation in cowpea in Brazil is UFLA03-84 (Bradyrhizobium sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00045-xDOI Listing
February 2019

Phenotypic characterization of swine peripheral blood monocyte-derived macrophages and ex vivo infection with Salmonella enterica serovar Typhimurium.

Braz J Microbiol 2019 Jan 24. Epub 2019 Jan 24.

Departmento de Clínica e Cirúrgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG, 31270-901, Brazil.

Macrophages are critical mediators of the inflammatory process, playing a relevant role in the pathogenesis of Salmonella Typhimurium. The protocols for isolation, culture, and differentiation of monocytes into macrophages and their interaction with Salmonella are well established in humans and murine models, but little information is available in swine. The aims of this study were to establish an efficient protocol for macrophage culture and to evaluate the interaction of the invA mutant strain and the wild type (WT) Salmonella Typhimurium with porcine macrophages. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00042-0DOI Listing
January 2019
1 Read

Combined application of biochar and PGPR consortia for sustainable production of wheat under semiarid conditions with a reduced dose of synthetic fertilizer.

Braz J Microbiol 2019 Jan 23. Epub 2019 Jan 23.

Department of Agronomy, FAS&T, Bahauddin Zakariya University, Multan, Pakistan.

This study investigates the combined effect of locally adopted plant growth promoting rhizobacteria (PGPR), biochar, and synthetic fertilizer on the wheat crop for the production and economic returns. A total of 20 PGPR strains were isolated from three different ecological zones of Pakistan and were evaluated. Of them, three isolates were selected for further studies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00043-zDOI Listing
January 2019
8 Reads

Evaluation of the presence of Paenibacillus larvae in commercial bee pollen using PCR amplification of the gene for tRNA.

Braz J Microbiol 2019 Jan 21. Epub 2019 Jan 21.

Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, 76010, Santiago de Querétaro, QRO, México.

American foulbrood (AFB) caused by Paenibacillus larvae is the most destructive honeybee bacterial disease and its dissemination via commercial bee pollen is an important mechanism for the spread of this bacterium. Because Mexico imports bee pollen from several countries, we developed a tRNA-PCR strategy and complemented that strategy with MALDI-TOF MS and amplicon-16S rRNA gene analysis to evaluate the presence of P. larvae in pollen samples. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00039-9DOI Listing
January 2019
5 Reads
0.592 Impact Factor

Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol.

Braz J Microbiol 2019 Jan 15;50(1):79-84. Epub 2019 Jan 15.

College of Pharmacy, Binzhou Medical University, Yantai, 264003, People's Republic of China.

Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-019-00040-2DOI Listing
January 2019

Characterization of multidrug-resistant Gram-negative bacilli isolated from hospitals effluents: first report of a bla-like in Klebsiella oxytoca, Algeria.

Braz J Microbiol 2019 Jan 19;50(1):175-183. Epub 2018 Dec 19.

Laboratoire de santé publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, QC, H9X 3R5, Canada.

The antibiotic susceptibility profile and antimicrobial resistance determinants were characterized on Gram-negative bacilli (GNB) isolated from Algerian hospital effluents. Among the 94 isolates, Enterobacteriaceae was the predominant family, with Escherichia coli and Klebsiella pneumoniae being the most isolated species. In non-Enterobacteriaceae, Acinetobacter and Aeromonas were the predominant species followed by Pseudomonas, Comamonas, Pasteurella, and Shewanella spp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0010-9DOI Listing
January 2019
2 Reads

Analysis of culturable microbiota present in the stomach of children with gastric symptoms.

Braz J Microbiol 2019 Jan 19;50(1):107-115. Epub 2018 Dec 19.

School of Basic Medical Science, Key Laboratory of Medical microbiology and parasitology of Education Department of Guizhou, Guizhou Medical University, No.1 South Dongqing Road, Guiyang, 550025, China.

Despite extensive studies on the gastric microbiota, including Helicobacter pylori and non-H. pylori, the bacterial composition in children remains unknown. In this study, we analyzed the culturable gastric bacteria in stomach biopsies from 346 children aged 1-15 years affected by gastric diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0030-5DOI Listing
January 2019
2 Reads
0.592 Impact Factor

Evaluation of growth and sporulation of a non-toxigenic strain of Clostridioides difficile (Z31) and its shelf viability.

Braz J Microbiol 2019 Jan 18;50(1):263-269. Epub 2018 Dec 18.

Veterinary School, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, Brazil.

The oral administration of non-toxigenic strains of Clostridioides difficile (NTCD) is currently showing promising results for the prevention of Clostridioides difficile infection (CDI) in humans and animals, and is being considered as a possible commercial product to be used in the near future. The aim of this work was to evaluate five culture media for the growth and sporulation of one NTCD (Z31) and evaluate the viability of a lyophilized spore solution of NTCD Z31 stored at 4 °C or at 25 °C for 2 years. Reinforced clostridial medium (RCM) and brain heart infusion broth (BHI) provided the highest production of NTCD Z31 spores. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0023-4DOI Listing
January 2019

Gonadal pathogenicity of an infectious bronchitis virus strain from the Massachusetts genotype.

Braz J Microbiol 2019 Jan 17;50(1):313-320. Epub 2018 Dec 17.

Departamento de Medicina Veterinária Preventiva, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, 05508-270, Brazil.

An outbreak of infectious bronchitis caused by the IBVPR03 strain of the Massachusetts genotype affected H-120 vaccinated laying hens in South Brazil. We investigated the cross protection of the vaccine by assessing the traqueal ciliostasis, virus recovery, and histopathological changes typically observed in the respiratory tract. Although the IBVPR03 strain is S1-genotyped as Massachusetts with a high genomic similarity to the H-120 vaccine strains, surprisingly, we found no tropism or pathogenicity to the trachea in birds infected with this strain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0007-4DOI Listing
January 2019
1 Read

Identification of key genes and signaling pathways during Sendai virus infection in vitro.

Braz J Microbiol 2019 Jan 17;50(1):13-22. Epub 2018 Dec 17.

Department of Physiology and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117456, Singapore.

Sendai virus (SeV) has been used as a model strain to reveal molecular features of paramyxovirus biology. In this study, we comprehensively analyzed the gene profiling of murine macrophages and airway epithelial cells in response to SeV using gene expression data. The significantly differentially expressed genes (DEGs) were screened by GEO2R. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0021-6DOI Listing
January 2019

Irradiation of reduced-sodium uncooked lamb sausage: antimicrobial efficacy and physicochemical impact.

Braz J Microbiol 2019 Jan 17;50(1):231-235. Epub 2018 Dec 17.

Universidade Federal do Rio de Janeiro- UFRJ, Av. Horácio Macedo, 2030, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, 21941-914, Brazil.

Excess sodium in food is associated with an increased risk of noncommunicable diseases. Faced with this reality, consumers are becoming aware of the importance of eating foods with reduced sodium content. This work assesses the effects of partial replacement of NaCl by KCl on the microbiological and physicochemical characteristics of uncooked lamb sausage treated with different doses of gamma radiation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0016-3DOI Listing
January 2019
8 Reads

Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test.

Braz J Microbiol 2019 Jan 13;50(1):139-145. Epub 2018 Dec 13.

Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700 - Centro, Alfenas, MG, CEP 37130-001, Brazil.

Sporotrichosis is an infection of the skin caused by traumatic inoculation of the fungus Sporothrix schenckii. Definitive diagnosis relies on direct visualization of the fungus or its isolation on culture medium, although both have low sensitivity. Alternatively, the detection of the antibody response offers a more rapid alternative for diagnosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0005-6DOI Listing
January 2019
2 Reads

Molecular characterization of Ehrlichia canis from naturally infected dogs from the state of Rio de Janeiro.

Braz J Microbiol 2019 Jan 11;50(1):1-12. Epub 2018 Dec 11.

Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR 465, km 7, Seropédica, Rio de Janeiro, 23890-000, Brazil.

The aim of the present study was to evaluate the genetic diversity of Ehrlichia canis in naturally infected dogs from six mesoregions of Rio de Janeiro state. E. canis was diagnosed with a real-time polymerase chain reaction (qPCR) targeting a 93 base pair (bp) fragment of the 16S rDNA gene. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0020-7DOI Listing
January 2019
14 Reads

Quantitative RT-PCR assays for identification and typing of the Equine encephalosis virus.

Braz J Microbiol 2019 Jan 10;50(1):287-296. Epub 2018 Dec 10.

The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.

Equine encephalosis (EE) is an acute, arthropod-borne, noncontagious, febrile disease of equids. The clinical signs of EE are similar to milder forms of African horse sickness (AHS) and the two diseases can be easily confused. The Equine encephalosis virus (EEV) is a distinct virus species within the genus Orbivirus, family Reoviridae, with ten linear segments of dsRNA genome. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0034-1DOI Listing
January 2019
2 Reads

Detection of Brucella spp. in dogs at Pantanal wetlands.

Braz J Microbiol 2019 Jan 10;50(1):307-312. Epub 2018 Dec 10.

Universidade Católica Dom Bosco (UCDB), Av. Tamandaré, 6000, Jardim Seminário, Campo Grande, Mato Grosso do Sul, CEP: 79117-900, Brazil.

Canine brucellosis is an infectious disease that produces reproductive disease in both males and females. Although Brucella canis is more common, the infection by Brucella abortus is more frequent in dogs sharing habitats with livestock and wild animals. We decided to investigate the role of dogs in the maintenance of Brucella spp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0006-5DOI Listing
January 2019
2 Reads

A protocol for selection of Trichoderma spp. to protect grapevine pruning wounds against Lasiodiplodia theobromae.

Braz J Microbiol 2019 Jan 7;50(1):213-221. Epub 2018 Dec 7.

Instituto Biológico, Alameda dos Vidoeiros, Campinas, Brazil.

There is no standardized protocol to select Trichoderma against trunk disease pathogens in grapevine. This study describes a protocol to select Trichoderma isolates for protecting the pruning wounds of grapevine against Lasiodiplodia theobromae. Thus, four assays were performed: (1) in vitro antagonism of Trichoderma isolates, molecularly identified, to control L. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0029-yDOI Listing
January 2019

Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids expressing S gene from porcine epidemic diarrhea virus and VP7 gene from porcine rotavirus.

Braz J Microbiol 2019 Jan 7;50(1):279-286. Epub 2018 Dec 7.

College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211#, Wenjiang District, Chengdu, 611130, Sichuan Province, China.

Porcine rotavirus (PoRV) and porcine epidemic diarrhea virus (PEDV) usually co-infect pigs in modern large-scale piggery, which both can cause severe diarrhea in newborn piglets and lead to significant economic losses to the pig industry. The VP7 protein is the main coat protein of PoRV, and the S protein is the main structural protein of PEDV, which are capable of inducing neutralizing antibodies in vivo. In this study, a DNA vaccine pPI-2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0022-5DOI Listing
January 2019
9 Reads

Improved blood culture workflow for faster identification of KPC-producing Enterobacterales.

Braz J Microbiol 2019 Jan 6;50(1):127-132. Epub 2018 Dec 6.

School of Pharmacy, Clinical Microbiology and Antimicrobial Resistance Laboratory, University of São Paulo, Av. Professor Lineu Prestes, 580, São Paulo, SP, Zip code 05508-900, Brazil.

Carba-NP original report for blood cultures described the need of subculture and mechanical lysis before testing, reaching the turnaround time of approximately 4 hours for sample preparation. We tested 100 consecutive blood cultures positive for Gram-negative bacilli on the Gram stain from a large clinical laboratory. Bacterial pellets were prepared by centrifugation and submitted to Carba-NP and Blue-Carba tests and used further to prepare smears for Vitek MS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0037-yDOI Listing
January 2019
6 Reads

Nano-multilamellar lipid vesicles (NMVs) enhance protective antibody responses against Shiga toxin (Stx2a) produced by enterohemorrhagic Escherichia coli strains (EHEC).

Braz J Microbiol 2019 Jan 6;50(1):67-77. Epub 2018 Dec 6.

Vaccine Development Laboratory, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, Cidade Universitária, São Paulo, SP, 05508-900, Brazil.

Microlipid vesicles (MLV) have a broad spectrum of applications for the delivery of molecules, ranging from chemical compounds to proteins, in both in vitro and in vivo conditions. In the present study, we developed a new set of nanosize multilayer lipid vesicles (NMVs) containing a unique combination of lipids. The NMVs enable the adsorption of histidine-tagged proteins at the vesicle surface and were demonstrated to be suitable for the in vivo delivery of antigens. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s42770-018-0035-0DOI Listing
January 2019