Search our Database of Scientific Publications and Authors

I’m looking for a

    8723 results match your criteria Bratislavske lekarske listy[Journal]

    1 OF 175

    Establishment of reverse transcription polymerase chain reaction assay for simultaneous detection of human parainfluenza virus types 1 to 4 in children with influenza like illnesses.
    Bratisl Lek Listy 2017 ;118(12):772-776
    Objectives: The aim of this study was to develop an in-house multiplex reverse transcription polymerase chain reaction (mRT-PCR), which can recognize HPIV1-4 in clinical samples.

    Background: Human parainfluenza virus (HPIV) is one of the major causes of viral respiratory infections and can affect people at any age, especially infants and young children.

    Methods: Four sets of specific primers targeting conserved areas of hemagglutinin-neuraminidase (HN) genes of HPIV1-4, were designed and tested with type-related plasmid controls. Read More

    Therapeutic hypothermia and myocardium in perinatal asphyxia: a microvolt T-wave alternans and Doppler echocardiography study.
    Bratisl Lek Listy 2017 ;118(12):765-771
    Objectives: This is the first study evaluating the predictive value of myocardial performance on arrhythmia and mortality via tissue-Doppler and microvolt T-wave alternans in infants with hypoxic-ischemic encephalopathy treated with therapeutic hypothermia-rewarming.

    Methods: The study included 23 term newborns having criteria for hypoxic-ischemic encephalopathy, and 12 controls. Tissue-Doppler imaging and T-wave alternans were performed in the first six hours after birth in patients from both groups and after hypothermia-rewarming treatment on the fifth day. Read More

    Effects of 2100 MHz radio frequency radiation on ductus epididymis tissue in rats.
    Bratisl Lek Listy 2017 ;118(12):759-764
    Purpose: The use of mobile phones is widespread since the beginning of 1990s. A great debate exists about the possible damage that the Radio Frequency - RF radiation from mobile phones exerts on different organs. The objective of this study was to investigate the possible histopathological effects of 2100 MHz RF radiation on rat ductus epididymis tissue using a light microscopy and immunohistochemical method after one or two month exposure. Read More

    Effects of epidermal growth factor on reduction of the formation of thrombus and vessel wall healing in an experimental rat model.
    Bratisl Lek Listy 2017 ;118(12):752-758
    Objectives: The aim of the current study was to investigate the synergistic effects of epidermal growth factor (EGF) and Enoxaparin in thrombus resolution.

    Method: Forty rats were divided into five groups (n = 8/group). Thrombosis was induced in all groups except the Sham group. Read More

    Periodontitis aggravates kidney damage in obese mice by MMP2 regulation.
    Bratisl Lek Listy 2017 ;118(12):740-745
    Objective:   To investigate the effect of periodontitis on the development of kidney damage in obese mice and its possible mechanism.

    Methods:   C57 BL/6J mice were fed high‑fat (HF) or low‑fat (LF) diet and then divided into four groups:  obesity with periodontitis (HFP), obesity without periodontitis (HFC), normal mice with periodontitis (LFP) and normal mice without periodontitis (LFC). Serum indicators of renal function, namely serum total protein (TP), albumin (ALB), creatinine (Cr) and blood urea nitrogen (UREA) were measured. Read More

    Wound infections after median sternotomy treated by VAC therapy, summary of results, and risk factor analysis.
    Bratisl Lek Listy 2017 ;118(12):736-739
    Introduction: The aim of this study is to summarize results and analyze risk factors for the development of wound infection in heart surgery patients after median sternotomy.

    Method: In this retrospective analysis with assessment of multiple risk factors, we examined 143 patients with infection after median sternotomy treated with VAC therapy from total of 4,650 patients operated in our department from 2012 to 2015.

    Results: Total of 143 patients developed significant SSI treated by VAC therapy following cardiac surgery. Read More

    Conservative management of biopsy confirmed high-grade squamous intraepithelial lesions.
    Bratisl Lek Listy 2017 ;118(12):732-735
    Objectives: Follow-up of women with biopsy-confirmed CIN2+ who were either treated immediately with LLETZ or managed conservatively to determine the rates of patients back on routine screening programme after a median of three years in two groups.

    Methods: In this retrospective study, 310 patients were involved who had undergone biopsy with result of CIN2+ between January 2011 and December 2014. Depending on the management, i. Read More

    Is the prevalence of the medication-related osteonecrosis of the jaws underestimated, evaluation in oncological and non-oncological disease.
    Bratisl Lek Listy 2017 ;118(12):724-731
    Objectives: The purpose of this study was to evaluate the prevalence of medication-related osteonecrosis of the jaw in Slovak population and compare the literature findings, whether the prevalence of MRONJ is underestimated.

    Background: Antiresorptive drugs significantly increase quality of life, although during therapy, or in post-treatment period, osteonecrosis of the jaws might occur as a severe adverse effect. Medication-related osteonecrosis of the jaws (MRONJ) is a severe problem that has been observed in the past few years. Read More

    Cardiac telocytes as principal interstitial cells for myocardial reparation and regeneration after infarction - our hope.
    Bratisl Lek Listy 2017 ;118(12):721-723
    According to our knowledge, this is the first research experiment that focuses on the study of the distribution of c-kit positive cells at the sites of myocardial infarction in human hearts (Fig. 3, Ref. 16). Read More

    Paraoxonase activity in sera of patients with non-alcoholic fatty liver disease.
    Bratisl Lek Listy 2017 ;118(12):719-720
    The results of our study showed significantly decreased levels of PON1 in patients with chronic liver diseases (controls 185 ± 14 U/l, NAFLD 160 ± 15 U/l, chronic hepatitis 99 ± 18 U/l, cirrhosis 52 ± 11 U/l). There were significant correlations of PON activities with standard liver function tests (Tab. 1, Ref. Read More

    The influence of cranio-cervical rehabilitation in patients with myofascial temporomandibular pain disorders.
    Bratisl Lek Listy 2017 ;118(11):710-713
    Objective: The aim of the study was to prove the causality between the craniocervical dysfunction and myofascial pain in the head and neck and to demonstrate the clinical value and usefulness of physiotherapy as one of the therapeutic options for myofascial pain.

    Methods: The group of patients diagnosed with myofascial dysfunctional pain syndrome contained 98 patients out of which 79 patients (81 %) were females and 19 patients (19 %) were males. The majority of the patients were aged between 26 and 35 years; the total age range was 14-77 years with the average of 38 years. Read More

    Periostin normalizes levels of cardiac markers in rats with experimental isoproterenol cardiotoxicity.
    Bratisl Lek Listy 2017 ;118(11):705-709
    Background: Although the molecular mechanism of cardiac healing is not fully understood, myocardial infarction is one of the most usual diagnoses in hospitalized patients in industrialized nations while periostin has been recently suggested to have a potential in tissue repairing following myocardial ischemia.

    Objectives: To investigate the effects of periostin on the levels of selected cardiac parameters (cardiac troponin I and T, creatine kinase and creatine kinase isoenzyme-MB), antioxidant/lipid peroxidation parameters (superoxide dismutase, catalase, and malondialdehyde), hepatic parameters (alkaline phosphatase, lactate dehydrogenase, aspartate and alanine transaminases) as well as lipids (total cholesterol, triglyceride, high, low and very‑low‑density lipoproteins) in a rat model of isoproterenol---induced myocardial injury.

    Methods: A total of 84 male rats were grouped into saline (Group I), periostin (Group II), isoproterenol (Group III) and isoproterenol+periostin (Group IV) groups (n = 21). Read More

    Optimization of Eva Green real-time mPCR for differentiating C. jejuni/coli directly from feces.
    Bratisl Lek Listy 2017 ;118(11):702-704
    Objective: To develop and optimize a rapid molecular method for diagnosing campylobacteriosis directly from a clinical fecal sample and at the same time for determining the most common causing agents - C. jejuni/coli.

    Materials And Methods: 38 clinical fecal samples from hospitalized patients with diarrheal syndrome were tested using a rapid immunochromatographic test. Read More

    Effect of EGF on Bax, Bcl-2 and Fas expression in ulcerous disease and N87 cell line.
    Bratisl Lek Listy 2017 ;118(11):695-701
    Background: To evaluate the effect of epithelial growth factor (EGF) in primary culture of ulcer patients and N87 cell line on expressions of apoptotic genes.

    Methods: Ulcer patients who attended Gastroenterology Clinic of Mersin University Medical Faculty were included in this study. Three different doses of EGF were applied to the primary culture of biopsy samples from ulcer patients and gastric cancer cell-line (ATCC-NCI-N87) . Read More

    Decreased circulatory microRNA-4478 as a specific biomarker for diagnosing non-ST-segment elevation myocardial infarction (NSTEMI) and its association with soluble leptin receptor.
    Bratisl Lek Listy 2017 ;118(11):684-690
    Objective: This study aimed to assess the usefulness of circulatory microRNA-4478 (miR-4478) and soluble leptin receptor (sLEPR) in prognosis and diagnosis of non-ST-segment elevation myocardial infarction (NSTEMI) and to introduce miR-4478 as a new biomarker for NSTEMI disease. This study aimed also to examine correlation between miR-4478 and soluble leptin receptor and effects of miR-4478 on leptin receptor concentration.

    Background: MicroRNAs could be used as predictive biomarkers for diseases. Read More

    Inhibition by Egb761 of the effect of cellphone radiation on the male reproductive system.
    Bratisl Lek Listy 2017 ;118(11):676-683
    Objectives: To determine the effects of Egb761 on testicular tissues and semen parameters in rats exposed to cellphone waves.

    Background: EGb761 has antioxidant properties as a free-radical scavenger. Cellphone electromagnetic radiation (EMR) induces oxidative stress in cells. Read More

    Effect of aquaporin 1 and 4 on masticatory muscles degeneration as a result of aging.
    Bratisl Lek Listy 2017 ;118(11):669-675
    Objective: The changes in the mouth structures due to aging cause some structural and functional changes by affecting masticatory muscles over time. The aim of this study was to evaluate the aging-related histopathologic changes and immunohistochemically assessed aquaporin 1 and 4 expressions on masseter and temporal muscles.

    Material And Methods: 14 Balb/c white mice (50-80 g) were used in this study. Read More

    The influence of orthodontic non-extraction treatment on the change in the inclination and position of incisors in the Europoid race.
    Bratisl Lek Listy 2017 ;118(11):662-668
    Aim: To detect post-treatment change in the inclination and position of incisors in cases treated with orthodontic non- extraction therapy.

    Materials And Methods: The group consisted of 102 patients without extractions in lower and upper dental arch during orthodontic treatment. Cephalogram examination evaluated the position of the lower incisor to point A by Downs-pogonion line (-1 to APo) and inclination of the lower incisor to mandibular line (-1 to ML), position of the upper incisor to nasion-pogonion line (+1 to NPo), inclination of the upper incisor to nasion-sella line (+1 to NS) and the size of the inter-incisival angle between upper and central lower incisor (-1 to +1). Read More

    Alpha-2-macroglobulin and hyaluronic acid as fibromarkers in patients with chronic hepatitis C.
    Bratisl Lek Listy 2017 ;118(11):658-661
    Background: Liver fibrosis is the final common pathway of chronic liver diseases of various etiology. From the practical standpoint, it would be ideal to have a noninvasive fibromarker. The aim of our study was to investigate the levels of alpha-2-macroglobulin, potential fibromarker, in correlation to histological staging and another potential fibromarker, hyaluronic acid, in patients with chronic hepatitis C. Read More

    Testosterone replacement therapy (TRT) and its effect on bone marrow. How serious is it and is there a true polyglobulia?
    Bratisl Lek Listy 2017 ;118(11):654-657
    Introduction: TRT in men with testosterone deficiency syndrome (TDS) had multiple positive effects and restore a quality of life of affected men. Polyglobulia is the most common dose-limiting adverse effect of TRT, but the mechanisms of TRT-mediated erythropoesis remain unclear. In this study, we evaluated long term haematological side effects of TRT: polyglobulia, elevated hemoglobin (Hb) and haematocrit (Ht). Read More

    Prevalent placement error of deep brain stimulation electrode in movement disorders (technical considerations).
    Bratisl Lek Listy 2017 ;118(11):647-653
    Background: Deep brain stimulation is an effective and safe technique. Displacement of the electrode relative to the optimal stimulation site can lead to insufficient effect and sometimes to the need of operative electrode re-position.

    Objective: This study was aimed to analyse targeting accuracy of deep brain stimulation electrode implantation to subthalamic nucleus (STN) and globus pallidus internus (Gpi). Read More

    FOXP3, RORγt and IL-10 cytokine profile in chronic heart failure.
    Bratisl Lek Listy 2017 ;118(10):637-641
    Background: Distinct subsets of T cells play crucial regulatory roles in inflammatory processes of chronic heart failure (CHF). Retinoic acid receptor-related orphan receptor-γt (Ror-γt) and Forkhead box P3 (Foxp3) have been defined as the "master regulators" of Th17 cells and Treg cells, respectively. At the same time, anti-inflammatory cytokines such as IL-10 may neutralize inflammation in CHF. Read More

    MEG3 long non-coding RNA prevents cell growth and metastasis of osteosarcoma.
    Bratisl Lek Listy 2017 ;118(10):632-636
    Objective: This study aimed to investigate the role of long non-coding RNA MEG3 (lncRNA MEG3) in osteosarcoma (OS) and further explore the underlying molecular mechanism.

    Materials And Methods: The expression profiles of MEG3 in OS cell lines and normal osteoblast cell line were detected by qRT-PCR. MEG3 was over-expressed in OS cell line by using LV-MEG3. Read More

    The protective effect of thymoquinone over olanzapine-induced side effects in liver, and metabolic side effects.
    Bratisl Lek Listy 2017 ;118(10):618-625
    Objectives: The aim of the study was to investigate the possible protective qualities of thymoquinone (TQ) against the side-effects of olanzapine (OLZ) in an experimental model in rat liver with histologic and biochemical assessments.

    Methods: Experimental procedures were performed on 35 female Sprague Dawley rats. Rats were randomly divided into five groups as: group 1: control; group 2: OLZ; group 3: OLZ+TQ-1; group 4: OLZ+TQ-2; and group 5: OLZ+TQ-3. Read More

    Hemodynamic effects of epinephrine in rats: evaluation by impedance cardiography.
    Bratisl Lek Listy 2017 ;118(10):613-617
    Background And Objectives: This study was aimed to examine how inotropic effects of intravenously injected epinephrine change thoracic impedance measurements and to reveal the possible effects of this change on other hemodynamic parameters by using the technique of impedance cardiography.

    Methods: 10 male Wistar Albino rats were divided into two equal groups: control and epinephrine. 0. Read More

    Proprotein convertase 1 mediated proneuropeptide proteolytic processing in ischemic neuron injury.
    Bratisl Lek Listy 2017 ;118(10):609-612
    Background: Pro-protein processing mechanism plays an important role in neuron injury.

    Objective: To study the protein convertase 1 (PC1) mediated processing mechanism, the ischemic cellular or tissue proPC1/PC1 or proCgA/CgA (pro-chromogranin A) was analyzed.

    Methods: NS20Y differentiated cells were stressed by 0-6 h of oxygen and glucose deprivation (OGD) in glucose-free DMEM and an anaerobic jar environment. Read More

    Immunohistochemical investigation of P16, P53 and Ki-67's prognostic values in diffuse large B-Cell lymphomas.
    Bratisl Lek Listy 2017 ;118(10):602-608
    Aim: The aim of this study is to determine the immunohistochemical properties of Ki-67, P53 expression and loss of P16, and to assess their relationship with both clinical parameters and patient survival in DLBCL.

    Method: Forty patients, diagnosed at the Pathology Department of our institute with nodal DLBCL were selected as the study group. The relationship between P16, P53, Ki-67 expressions and clinical and laboratory parameters like age, gender, performance status, Eastern Cooperative Oncology Group (ECOG), clinical stage, presence of B-symptoms, bone marrow involvement, International Prognostic Index (IPI) score, lactate dehydrogenase (LDH) level, extranodal extension, relapse, C-reactive protein (CRP), sedimentation, number of leukocytes in patients and patient survival were then statistically evaluated. Read More

    Effect of lipopolysaccharide on toll-like receptor-4 signals in mouse cancer cells.
    Bratisl Lek Listy 2017 ;118(10):598-601
    Background: Recent findings showed that activated TLR signals on cancer cells might promote cancer progression. This study was designed to explore the influence of Toll-like receptor 4 (TLR4) agonist lipopolysaccharides (LPS) on mouse melanoma and breast cancer cell proliferation and their TLR4 signalling.

    Methods: Mouse melanoma cell line (B16F10) and breast cancer cell line (4T1) were taken as models. Read More

    Comparison between the effect of kartogenin and TGFβ3 on chondrogenesis of human adipose- derived stem cells in fibrin scaffold.
    Bratisl Lek Listy 2017 ;118(10):591-597
    Background: Due to very sluggish turnover at the molecular and cellular level, the healing of chondral damages has been considered difficult. In the current study, the effects of the Kartogenin, a small heterocyclic molecule on chondrogenic differentiation of stem cells was compared to TGF-β3.

    Methods: Human Adipose-Derived Stem Cells were extracted during an elective surgery. Read More

    Investigation of the effectiveness of ghrelin treatment in lung tissue of rats with sepsis.
    Bratisl Lek Listy 2017 ;118(10):585-590
    Objective: We aimed to investigate the effects of exogenous ghrelin on cytokine and ghrelin levels, oxidant parameters, and apoptotic genes in lung tissue during sepsis.

    Background: There was evidence that changes of apoptosis are linked with morbidity and mortality in sepsis. There were scarce studies on the effect of ghrelin on apoptotic genes and endogenous ghrelin levels during sepsis. Read More

    Comparative analysis of human omental milky spots between the patients with colon cancer and the control group.
    Bratisl Lek Listy 2017 ;118(10):580-584
    Aim: Morphological description of milky spots (MSs) in the human greater omentum.

    Method: Samples of the greater omentum collected during surgical procedures were subjected to further histological analysis. Two groups of patients were studied. Read More

    Iron deposition in rabbit cerebellum after exposure to generated and mobile GSM electromagnetic fields.
    Bratisl Lek Listy 2017 ;118(10):575-579
    Background: Mobile phone application may cause structural, functional changes and accumulation of toxic elements in brain.

    Objectives: The aim of this study was to investigate iron accumulation in rabbit cerebellum after exposure to RF EMF with light and scanning electron microscopy.

    Materials And Methods: Histochemical analysis of iron distribution by light and electron microscopy with energy-dispersive microanalysis was used. Read More

    Improving the potency of DNA vaccine encoding HIV-1 Nef antigen using two endogenous adjuvants in mouse model.
    Bratisl Lek Listy 2017 ;118(9):564-569
    Background: DNA immunization can induce long-term immune responses, which are required to design an effective HIV vaccine. It was shown that antigen-expressing plasmids can increase the protective immunity against infectious diseases such as: influenza and malaria. However, DNA-based immunizations have poor immunogenicity, thus the use of potent immunoadjuvants can enhance their potency. Read More

    Protective effect of ischemic preconditioning on testis injury following transient focal cerebral ischemia in diabetic rats.
    Bratisl Lek Listy 2017 ;118(9):557-563
    Objectives: We aim to evaluate the effect of ischemic preconditioning (IPreC) on testicular tissue after transient middle cerebral artery occlusion (MCAo) in Streptozotocin-induced diabetic (STZ) and non-diabetic rats.

    Methods: Testis injury and alterations of testosterone levels were evaluated histologically.. Read More

    Effects of N-acetyl cysteine to improve acute lung injury in rats.
    Bratisl Lek Listy 2017 ;118(9):552-556
    Aim: To study the mechanism of NAC to improve LPS induced acutelung lung injury.

    Methods: The 40 rats were divided into 4 groups included NC group, Model group, NAC group and DXM group. The rats of Model, NAC and DXM groups were injected LPS, NAC group and DXM group were resepectively injected NAC (200 mg/kg) or DXM (70 mg/kg). Read More

    Clinical and histopathological relationship of sildenafil and bosentan treatments in rats with monocrotaline induced pulmonary hypertension.
    Bratisl Lek Listy 2017 ;118(9):544-551
    Background: Pulmonary arterial hypertension (PAH) is a challenging disorder characterized by increasing pulmonary artery pressure, which is hard to treat.

    Objective: This study was aimed to investigate the effects of bosentan, sildenafil and their combination.

    Methods: Saline or MCT were applied to Wistar rats. Read More

    Metabolic disturbances induce malignant heart arrhythmias in rats.
    Bratisl Lek Listy 2017 ;118(9):539-543
    Objectives: Metabolic disturbances are considered to condition the occurrence of malignant heart arrhythmias and negatively influence the chances of a patient to survive. To test this assumption, a model of metabolic syndrome was selected in which rats were receiving a diet resembling that of the westernized population.

    Background: Metabolic syndrome is a comorbidity of major cardiovascular risk factors (dyslipidemia, hypertension, impaired glucose tolerance or insulin resistance, diabetes mellitus, and obesity), all facilitating cardiovascular complications leading to morbidity and mortality of patients. Read More

    Relationship between ncRNAs and gastric cancer: meta-analysis.
    Bratisl Lek Listy 2017 ;118(9):535-538
    Objective: This study was designed to investigate the action and mechanism of cordyceps polysaccharide on rat acute liver failure (ALF).

    Methods: Sixty rats were randomly divided into five groups: normal group, model group, and cordyceps polysaccharide groups with high, middle and low doses (20, 10 and 5 mg/ml). Apoptosis was detected through TUNEL method. Read More

    Bone metastases in neuroendocrine tumors.
    Bratisl Lek Listy 2017 ;118(9):529-534
    Neuroendocrine tumors arise from various cells that form a part of the endocrine system and account for a small number of cases encountered by oncologists in clinical practice. The clinical incidence of these tumors used to be low, and newer imaging modalities have now begun to be used for detecting bone metastases at an earlier stage. Bone metastases arising from neuroendocrine tumors are a well-recognized complication. Read More

    The significance of miR-145 in the prediction of preeclampsia.
    Bratisl Lek Listy 2017 ;118(9):523-528
    Aim: The aim of this study was to explain the effects of miRNA-145 in the pathogenesis of preeclampsia.

    Methods: Collecting the placental tissue of 40 severe preeclampsia patients and 20 normal pregnant women, and observation of the pathological findings by HE staining. Measuring the miR-145 by RT-PCR. Read More

    Analysis of HLA-G gene polymorphisms in Slovak women with pre-eclampsia.
    Bratisl Lek Listy 2017 ;118(9):517-522
    Objectives: To identify possible association between the selected HLA-G gene polymorphisms and risk of pre-eclampsia.

    Background: Pre-eclampsia is a serious multisystem disorder that affects women during pregnancy. Despite many research studies, the pathology of pre-eclampsia is not fully understood. Read More

    Value of neutrophil to lymphocyte and platelet to lymphocyte ratios in pneumonia.
    Bratisl Lek Listy 2017 ;118(9):513-516
    Purpose: In our study, we aimed to evaluate neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) in patients with Community-acquired pneumonia (CAP).

    Methods: This is a retrospective study consisting of 114 patients with CAP and 50 control subjects. Patients with CAP were divided into 2 groups, as inpatient and outpatient. Read More

    Neutrophil-to-lymphocyte ratio as a predictor of preoperative tumor staging in testicular germ cell tumors.
    Bratisl Lek Listy 2017 ;118(9):510-512
    Objectives: The aim of our study was to evaluate associations of elevated preoperative neutrophil-to-lymphocyte ratio (NLR) with testicular germ cell tumors (GCT) characteristics other than cancer specific survival (CSS) and progression free survival (PFS).

    Background: NLR was recently presented as a widely available and inexpensive marker of poor prognosis in several types of solid tumors. Previous study showed no predictive value of NLR for CSS and PFS in testicular GCT. Read More

    Some antidiabetic medicinal plants used by traditional healers in Ado Ekiti, Nigeria.
    Bratisl Lek Listy 2017 ;118(8):504-505
    Objectives: The work described plants collection, preparation and administration for diabetes cure in Ado Ekiti in Nigeria.

    Methods: Twenty three plant samples were identified for the use in the treatment of diabetes by the herbalists. The plants that are common among the healers are Anthocleista djalonensis, Vernonia amygdalina, Ocimum gratissimum, Momordica charantia. Read More

    MiR-26a regulates vascular smooth muscle cell calcification in vitro through targeting CTGF.
    Bratisl Lek Listy 2017 ;118(8):499-503
    Vascular calcification is one of the most important factors for high morbidity and mortality from cardiovascular and cerebrovascular diseases. The aim of this study is to investigate the effect and mechanism of miR-26a on vascular smooth muscle cell calcification. First, the VSMCs were induced by β-glycerol phosphate (β-GP) for 7d and 14d, and Alizarin Red S staining was performed to examine the mineralized nodule change; then real time RT-PCR and western blotting were performed to explore the expression of miR-26a, CTGF, OPG, RANKL and ALP in un-induced and β-GP-induced VSMCs; next, the VSMCs were transfected with miR-26a mimics, and Alizarin Red S staining was performed to examine the mineralized nodule change; finally, real time RT-PCR and western blotting were performed to explore the expression of miR-26a, CTGF, OPG, RANKL and ALP in un-transfected and miR-26a mimics transfected VSMCs. Read More

    1 OF 175