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    8688 results match your criteria Bratislavske lekarske listy[Journal]

    1 OF 174

    Improving the potency of DNA vaccine encoding HIV-1 Nef antigen using two endogenous adjuvants in mouse model.
    Bratisl Lek Listy 2017 ;118(9):564-569
    Background: DNA immunization can induce long-term immune responses, which are required to design an effective HIV vaccine. It was shown that antigen-expressing plasmids can increase the protective immunity against infectious diseases such as: influenza and malaria. However, DNA-based immunizations have poor immunogenicity, thus the use of potent immunoadjuvants can enhance their potency. Read More

    Protective effect of ischemic preconditioning on testis injury following transient focal cerebral ischemia in diabetic rats.
    Bratisl Lek Listy 2017 ;118(9):557-563
    Objectives: We aim to evaluate the effect of ischemic preconditioning (IPreC) on testicular tissue after transient middle cerebral artery occlusion (MCAo) in Streptozotocin-induced diabetic (STZ) and non-diabetic rats.

    Methods: Testis injury and alterations of testosterone levels were evaluated histologically.. Read More

    Effects of N-acetyl cysteine to improve acute lung injury in rats.
    Bratisl Lek Listy 2017 ;118(9):552-556
    Aim: To study the mechanism of NAC to improve LPS induced acutelung lung injury.

    Methods: The 40 rats were divided into 4 groups included NC group, Model group, NAC group and DXM group. The rats of Model, NAC and DXM groups were injected LPS, NAC group and DXM group were resepectively injected NAC (200 mg/kg) or DXM (70 mg/kg). Read More

    Clinical and histopathological relationship of sildenafil and bosentan treatments in rats with monocrotaline induced pulmonary hypertension.
    Bratisl Lek Listy 2017 ;118(9):544-551
    Background: Pulmonary arterial hypertension (PAH) is a challenging disorder characterized by increasing pulmonary artery pressure, which is hard to treat.

    Objective: This study was aimed to investigate the effects of bosentan, sildenafil and their combination.

    Methods: Saline or MCT were applied to Wistar rats. Read More

    Metabolic disturbances induce malignant heart arrhythmias in rats.
    Bratisl Lek Listy 2017 ;118(9):539-543
    Objectives: Metabolic disturbances are considered to condition the occurrence of malignant heart arrhythmias and negatively influence the chances of a patient to survive. To test this assumption, a model of metabolic syndrome was selected in which rats were receiving a diet resembling that of the westernized population.

    Background: Metabolic syndrome is a comorbidity of major cardiovascular risk factors (dyslipidemia, hypertension, impaired glucose tolerance or insulin resistance, diabetes mellitus, and obesity), all facilitating cardiovascular complications leading to morbidity and mortality of patients. Read More

    Relationship between ncRNAs and gastric cancer: meta-analysis.
    Bratisl Lek Listy 2017 ;118(9):535-538
    Objective: This study was designed to investigate the action and mechanism of cordyceps polysaccharide on rat acute liver failure (ALF).

    Methods: Sixty rats were randomly divided into five groups: normal group, model group, and cordyceps polysaccharide groups with high, middle and low doses (20, 10 and 5 mg/ml). Apoptosis was detected through TUNEL method. Read More

    Bone metastases in neuroendocrine tumors.
    Bratisl Lek Listy 2017 ;118(9):529-534
    Neuroendocrine tumors arise from various cells that form a part of the endocrine system and account for a small number of cases encountered by oncologists in clinical practice. The clinical incidence of these tumors used to be low, and newer imaging modalities have now begun to be used for detecting bone metastases at an earlier stage. Bone metastases arising from neuroendocrine tumors are a well-recognized complication. Read More

    The significance of miR-145 in the prediction of preeclampsia.
    Bratisl Lek Listy 2017 ;118(9):523-528
    Aim: The aim of this study was to explain the effects of miRNA-145 in the pathogenesis of preeclampsia.

    Methods: Collecting the placental tissue of 40 severe preeclampsia patients and 20 normal pregnant women, and observation of the pathological findings by HE staining. Measuring the miR-145 by RT-PCR. Read More

    Analysis of HLA-G gene polymorphisms in Slovak women with pre-eclampsia.
    Bratisl Lek Listy 2017 ;118(9):517-522
    Objectives: To identify possible association between the selected HLA-G gene polymorphisms and risk of pre-eclampsia.

    Background: Pre-eclampsia is a serious multisystem disorder that affects women during pregnancy. Despite many research studies, the pathology of pre-eclampsia is not fully understood. Read More

    Value of neutrophil to lymphocyte and platelet to lymphocyte ratios in pneumonia.
    Bratisl Lek Listy 2017 ;118(9):513-516
    Purpose: In our study, we aimed to evaluate neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) in patients with Community-acquired pneumonia (CAP).

    Methods: This is a retrospective study consisting of 114 patients with CAP and 50 control subjects. Patients with CAP were divided into 2 groups, as inpatient and outpatient. Read More

    Neutrophil-to-lymphocyte ratio as a predictor of preoperative tumor staging in testicular germ cell tumors.
    Bratisl Lek Listy 2017 ;118(9):510-512
    Objectives: The aim of our study was to evaluate associations of elevated preoperative neutrophil-to-lymphocyte ratio (NLR) with testicular germ cell tumors (GCT) characteristics other than cancer specific survival (CSS) and progression free survival (PFS).

    Background: NLR was recently presented as a widely available and inexpensive marker of poor prognosis in several types of solid tumors. Previous study showed no predictive value of NLR for CSS and PFS in testicular GCT. Read More

    Some antidiabetic medicinal plants used by traditional healers in Ado Ekiti, Nigeria.
    Bratisl Lek Listy 2017 ;118(8):504-505
    Objectives: The work described plants collection, preparation and administration for diabetes cure in Ado Ekiti in Nigeria.

    Methods: Twenty three plant samples were identified for the use in the treatment of diabetes by the herbalists. The plants that are common among the healers are Anthocleista djalonensis, Vernonia amygdalina, Ocimum gratissimum, Momordica charantia. Read More

    MiR-26a regulates vascular smooth muscle cell calcification in vitro through targeting CTGF.
    Bratisl Lek Listy 2017 ;118(8):499-503
    Vascular calcification is one of the most important factors for high morbidity and mortality from cardiovascular and cerebrovascular diseases. The aim of this study is to investigate the effect and mechanism of miR-26a on vascular smooth muscle cell calcification. First, the VSMCs were induced by β-glycerol phosphate (β-GP) for 7d and 14d, and Alizarin Red S staining was performed to examine the mineralized nodule change; then real time RT-PCR and western blotting were performed to explore the expression of miR-26a, CTGF, OPG, RANKL and ALP in un-induced and β-GP-induced VSMCs; next, the VSMCs were transfected with miR-26a mimics, and Alizarin Red S staining was performed to examine the mineralized nodule change; finally, real time RT-PCR and western blotting were performed to explore the expression of miR-26a, CTGF, OPG, RANKL and ALP in un-transfected and miR-26a mimics transfected VSMCs. Read More

    Association between neutrophil/lymphocyte ratio with ductus arteriosus patency in preterm newborns.
    Bratisl Lek Listy 2017 ;118(8):491-494
    Objective: The aim of this study was to assess the influence of neutrophil to lymphocyte (N/L) ratio and platelet indices on patent ductus arteriosus (PDA) in preterm infants.

    Background: PDA is a common problem with potentially serious associated morbidities in preterm neonates.

    Methods: Premature infants with hemodynamically significant PDA (n = 47) and a control group without PDA (n = 50) who were hospitalized in the neonatal intensive care unit were retrospectively evaluated. Read More

    Use of graph algorithms in the processing and analysis of images with focus on the biomedical data.
    Bratisl Lek Listy 2017 ;118(8):485-490
    Introduction: Image segmentation is a known problem in the field of image processing. A great number of methods based on different approaches to this issue was created. One of these approaches utilizes the findings of the graph theory. Read More

    Long-term outcomes following minimal invasive versus conventional aortic valve replacement: a propensity match analysis.
    Bratisl Lek Listy 2017 ;118(8):479-484
    Introduction: Minimal invasive aortic valve replacement has become a routine procedure. In this study, we compared the outcomes between conventional and minimal invasive aortic valve replacement via the partial upper sternotomy that were performed in our Institution.

    Methods: The 5 year survival and postoperative outcomes of 34 patients that underwent isolated MIAVR between the years 2010-2013 were compared with the outcomes of 34 randomly selected patients that underwent conventional AVR, after propensity match analysis. Read More

    Giant inguinoscrotal hernia repair.
    Bratisl Lek Listy 2017 ;118(8):472-478
    Giant inguinoscrotal hernia is defined as an inguinal hernia extending below the midpoint of inner thigh in standing position. The authors describe giant inguinoscrotal hernia and small umbilical hernia with 12 years history of this uncommon disease. After preoperative evaluation, US and CT examination he was operated on. Read More

    eNOS and XRCC4 VNTR variants contribute to formation of nicotine dependence and/or schizophrenia.
    Bratisl Lek Listy 2017 ;118(8):467-471
    Background: This study aimed to evaluate whether VNTR variants in the Endothelial Nitric Oxide Synthase (eNOS) and the XRCC4 gene play any role in nicotine dependence (ND) and/or Schizophrenia+ND (Sch+ND) ethiopathogenesis.

    Methods: Present study included 100 individuals with ND, 60 patients with Sch+ND, and 70 healthy controls. These variants were analyzed using PCR. Read More

    Phthalate induced toxicity in prostate cancer cell lines and effects of alpha lipoic acid.
    Bratisl Lek Listy 2017 ;118(8):460-466
    Objective: The effects of dimethyl phthalate, diethyl phthalate, diisobutyl phthalate, di-n-butyl phthalate, benzylbutyl phthalate, di-2-ethylhexyl phthalate were investigated on human prostate cancer cell lines DU145 and PC3 in vitro.

    Materials And Methods: Standards of dimethyl phthalate, diethyl phthalate, di-isobutyl phthalate, dibutyl phthalate, benzyl butyl phthalate, and di-ethyl hexyl phthalate were used. Alpha lipoic acid was used as antioxidant compound. Read More

    The effect of sulforaphane on oxidative stress and inflammation in rats with toxic hepatitis induced by acetaminophene.
    Bratisl Lek Listy 2017 ;118(8):453-459
    Objective: The aim of the present study was to reveal the possible effect of sulforaphane on oxidative stress and inflammation in rats liver with toxic hepatitis induced by acetaminophene.

    Background: Sulforaphane is a compound with high antioxidant properties. Acetaminophen, which is a para-aminophenol derivative, can lead to fatal hepatic necrosis with direct hepatotoxic effects at high doses. Read More

    MicroRNA-497 suppress osteosarcoma by targeting MAPK/Erk pathway.
    Bratisl Lek Listy 2017 ;118(8):449-452
    Background: The aim of this study was to study the mechanism of miRNA-497 in the apoptosis of osteosarcoma cells.

    Methods: MG-63 cells were divided into the three groups: NC, BL and miRNA groups, NC group were treated with nothing; BL group were transfected with blank vector; miRNA group were transfected with miRNA-497. Cell proliferation rate was detected by MTT method; Apoptosis rate was detected by flow cytometry and measuring the gene and protein expression of MAPK, Erk and P 21 by RT-PCR and Western blot. Read More

    The effect of leptin and resveratrol on JAK/STAT pathways and Sirt-1 gene expression in the renal tissue of ischemia/reperfusion induced rats.
    Bratisl Lek Listy 2017 ;118(8):443-448
    Objective: Our study aimed to investigate the possible modifying effects of leptin and combined use of resveratrol on rat renal I/R injury and their relationship on signal pathways and apoptosis-related mechanisms.

    Background: Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure.

    Methods: Male Sprague Dawley rats were divided into 5 groups: Control, I/R, I/R+leptin, I/R+resveratrol and I/R+leptin+resveratrol. Read More

    Quercetin induces apoptosis and necroptosis in MCF-7 breast cancer cells.
    Bratisl Lek Listy 2017;118(2):123-128
    Objective: This study investigated the quercetin (Que) effects on growth of MCF-7 human cancer breast cell line and its cellular death mechanism.

    Background: Quercetin has been found to be very efficacious against many different types of cancer cells. However, the study is not sufficiently powered to demonastrate anticancer mechanisms. Read More

    The role of pegaptanib sodium in the suppression of epidural fibrosis in a postlaminectomy rat model.
    Bratisl Lek Listy 2017;118(2):118-122
    Objective: Spinal epidural fibrosis is a clinical condition that develops after laminectomy and can compress the spine. Many agents have been tried for the treatment, but none has entered clinical use at present. Pegaptanib sodium is an antiangiogenetic drug that prevents the development of new vessels and thus adhesion by inhibiting the effect of VEGF. Read More

    Role of ischemic modified albumin in the early diagnosis of increased intracranial pressure and brain death.
    Bratisl Lek Listy 2017;118(2):112-117
    Aim: Increased intracranial pressure following trauma and subsequent possible development of brain death are important factors for morbidity and mortality due to ischemic changes. We aimed to establish the role of ischemic modified albumin (IMA) in the early diagnosis of the process, starting with increased intracranial pressure and ending with brain death.

    Materials And Methods: Eighteen Wistar-Albino rats were divided into three groups; control (CG, n = 6), increased intracranial pressure (ICPG, n = 6), and brain death (BDG, n = 6). Read More

    Flow cytometric examination of apoptotic effect on brain tissue in postnatal period created by intrauterine oxcarbazepine and gabapentin exposure.
    Bratisl Lek Listy 2017;118(2):107-111
    Objective: For epileptics, pregnancy contains the balance between no seizure period and antiepileptic use having the least teratogenicity risk. The purpose is to analyse with flow cytometry the apoptotic effects on postnatal brain tissue caused by prenatal use of second generation antiepileptics oxcarbazepine (OXC) and gabapentin (GBP) having different effect mechanisms.

    Method: 30 (n = 5 each group) Wistar albino male rats (45-days-old) are used. Read More

    The effect of vitamin D on MCF-7 breast cancer cell metabolism.
    Bratisl Lek Listy 2017;118(2):101-106
    The role of vitamin D in calcium absorption and bone health is known. The studies revealed that vitamin D modulates breast cancer cell growth and it is also associated with a reduced breast cancer risk. The primary objective of this study was to highlight the metabolic effect of Vitamin D on MCF-7 breast cancer cell line. Read More

    Melatonin: a hepatoprotective agent against radioiodine toxicity in rats.
    Bratisl Lek Listy 2017;118(2):95-100
    Objectives: The aim of the current study was to investigate the possible radioprotective effects of melatonin against hepatic radioiodine (RAI) toxicity.

    Methods: Thirty-six rats were randomly divided into three groups: untreated control (Group 1); oral radioiodine (RAI, 111 MBq) administrated rats (Group 2), and melatonin group (oral RAI and daily intraperitoneal injection of 12 mg/kg melatonin-Group 3). In the third group, melatonin administration was started two days before and continued for five days after RAI administration. Read More

    Antioxidant therapy impresses in oxidative stress-induced kidney cells.
    Bratisl Lek Listy 2017;118(2):89-94
    Introduction:   Renal cell cancer (RCC) is not a single entity, but consists of different types of tumors derived from various parts of the nephron (epithelium or renal tubules). It is known that cancer initiation and progression is related to the balance between oxidants and antioxidants directly. Interestingly, advanced stages of cancer such as metastasis, angiogenesis are associated with cell oxidative capacity. Read More

    Cisplatin plus norcantharidin alter the expression of TGF-β1/Smads signaling pathway in hepatocellular carcinoma.
    Bratisl Lek Listy 2017;118(2):85-88
    Purpose: To investigate the effects of cisplatin plus norcantharidin on transforming growth factor (TGF)-β1/Smads signaling pathway in hepatocellular carcinoma cells.

    Methods: Hepatocellular carcinoma cells (Hep3B) were divided into four groups: control group, cisplatin 2.0 μg/ml group, norcantharidin 10 μg/ml group, and cisplatin 2. Read More

    Effects of sevoflurane on apoptosis, BDNF and cognitive functions in neonatal rats.
    Bratisl Lek Listy 2017;118(2):80-84
    Objective: To evaluate the early and late effects of sevoflurane on the neonatal brain.

    Background: Sevoflurane is the most used anaesthetics in neonatal subjects.

    Methods: The study included 7-day-old male Wistar-Albino rats (n = 30), which were divided into the two groups according to the anaesthetic received: sevoflurane (S) and control group (C). Read More

    Plasma nesfatin 1 level in patients with first attack psychosis.
    Bratisl Lek Listy 2017;118(2):77-79
    Objectives: This study aimed to investigate the possible relationship between plasma concentrations of nesfatin 1 and first attack psychosis.

    Methods: Totally, 32 patients with the first episode psychosis and 33 randomly selected weight- and body mass index-matched healthy volunteers admitted to Mustafa Kemal University, Faculty of Medicine, Training and Research Hospital, Psychiatry outpatient clinic were included. Healthy control subjects were matched in terms of weight and body mass index (BMI). Read More

    Differentially regulated ADAMTS1, 8, and 18 in gastric adenocarcinoma.
    Bratisl Lek Listy 2017;118(2):71-76
    Objective: The aim is to investigate the expression status of ADAMTS1,8, and 18 proteases in gastric cancer (GC) and lymphatic metastasis.

    Background: A disintegrin and metalloprotease with thrombospondin motifs (ADAMTS) is a new protease family, and has important biological functions such as hemostasis, extracellular matrix remodeling and regulation of angiogenesis.

    Methods: The immunostaining status of ADAMTS1,8, and 18 were investigated in formalin-fixed paraffin-embedded samples of 25 patients who underwent curative resection for GC. Read More

    Human race as indicator of 3D planning of soft tissue of face and multidisciplinary approach.
    Bratisl Lek Listy 2017 ;118(7):431-436
    Objective: The aim of this study was to determine the optimal parameters for 3D soft tissue planning for ortognatic treatment by gender and increases the effectiveness of multidisciplinary cooperation.

    Methods: Craniofacial parameters which were analysed: nose breadth (al-al), bi-entocanthion breadth (en-en), bi-zygomatic breadth (zy-zy), bi-gonial breadth (go-go), total facial height (n-gn), mouth breadth (ch-ch), morphologic face height (sn-gn), upper-lip height (Ls-Stm), lower-lip height (Stm-Li) and pupils - mid-face (right). The statistically significant level was determined at p values < 0. Read More

    Risk perception of NSAIDs in hospitalized patients in Greece.
    Bratisl Lek Listy 2017 ;118(7):427-430
    Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used analgesics worldwide in different syndromes. There is a relevant evidence about NSAIDs various adverse effects (AEs) on gastrointestinal, cardiovascular, renal, pulmonary, nervous systems. Many of these problems are preventable with respects to appropriate patient´s risk perception. Read More

    The significance of Akt/NF-κb signaling pathway in the posterior cataract animal model.
    Bratisl Lek Listy 2017 ;118(7):423-426
    Objective: To establish SD rat posterior capsular opacification (posterior capsular opacification- PCO) animal model, and to detect the expression of Akt/NF-kb signaling pathway in the PCO model.

    Methods: 30 healthy SD rats were randomly divided into control group (0d) and the experimental groups (7d and 14 d), there were 10 rats at all time points. All rats (right eye) were treated with the lens capsule, and the inflammatory reaction of the anterior segment of the eye and the occurrence of PCO at different time points were observed under the microscope. Read More

    Effect of different doses of pregabalin on skeletal muscle ischaemia-reperfusion injury in rats.
    Bratisl Lek Listy 2017 ;118(7):417-422
    Aim/introduction: Analgesic, anti-inflammatory and anti-apoptotic effects of pregabalin have been shown previously. In this study, we investigated the protective effect of different doses of pregabalin on skeletal muscle IR injury in rats.

    Materials And Methods: 24 rats were randomly divided into 4 groups (Control, Ischaemia-Reperfusion (IR), IR-Pregabalin 50 mg, IR-Pregabalin 200 mg). Read More

    The impact of sleep deprivation on hippocampal-mediated learning and memory in rats.
    Bratisl Lek Listy 2017 ;118(7):408-416
    Background: To examine the impact of different types of sleep deprivation on hippocampal-mediated learning and memory in rats.

    Methods: Forty-eight Sprague-Dawley male rats were randomly assigned to 1 of 4 equal-size groups: (1) 12 hours of sleep per day (control). (2) total sleep deprivation (TSD), (3) rapid eye movement (REM) deprivation (RD), and (4) sleep restricted to 4 hours per day (SR). Read More

    The impact of using different doses of progesterone on memory performance.
    Bratisl Lek Listy 2017 ;118(7):405-407
    Objective: Progesterone is a sex hormone and its receptors are expressed throughout the hippocampus. This study was aimed at evaluating the effects of different doses of progesterone on memory.

    Methods: Male rats were arbitrarily assigned to nine groups, namely Group I: control, Group II: control-cannula, Group III received 0. Read More

    Effects of granulocyte colony-stimulating factor administration time on pain.
    Bratisl Lek Listy 2017 ;118(7):399-404
    Objective: This study was aimed at evaluating the effect of administration time of granulocyte colony-stimulating factor (G-CSF) on the level of pain related to G-CSF.

    Methods: This study was carried out with 48 cancer patients divided into A and B groups. In the first stage of the study, the groups A and B were planned to be administered with G-CSF at 10: 00 and 14: 00, respectively. Read More

    Roles of adiponectin and leptin as diagnostic markers in pancreatic cancer.
    Bratisl Lek Listy 2017 ;118(7):394-398
    Ntroduction: Obesity is one of the most serious public health problem worldwide. Adipose tissue synthetize and secrete many growth factors and several cytokines known as adipokines. Studies demonstrated changes in the levels of these adipokines in many types of cancer associated with obesity. Read More

    Effects of ceranib-2 on cell survival and TNF-alpha in colon cancer cell line.
    Bratisl Lek Listy 2017 ;118(7):391-393
    Objective: The aim of this study was to investigate the effects of a novel anti-cancer drug, ceranib-2, which targets the acid ceramidase, in human colon cancer cell line.

    Materials And Methods: The cell lines were treated with 50 µM of ceranib-2. Relative mRNA expression of TNF-alpha, TNF-R1 and ASAH were assessed by quantitative RT-PCR. Read More

    Dose-dependent effects of adiponectin on ADAMTS-9 gene expression in human chondrocytes.
    Bratisl Lek Listy 2017 ;118(7):386-390
    Objective: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), comprising of 19 members is a family of peptidases. They have several vital functions in physiological and pathological processes in organisms. ADAMTS-9 has aggrecanolytic activity and is responsible for degradation of aggrecan mainly in articular cartilage. Read More

    The age at onset in Multiple Sclerosis is associated with patient's prognosis.
    Bratisl Lek Listy 2017 ;118(6):374-377
    Background: The causes of the individual differences in the rate of disability progression in multiple sclerosis (MS) are still not completely clear. According to the long-term prognosis of MS patients, the search for new valuable prognostic markers of "benign" or "malign" MS is necessary.

    Objectives: Our aim was to assess the possible association of MS onset age with the disease disability progression rate in Slovak patients with MS. Read More

    Impact of cleft lip and/or palate in children on family quality of life before and after reconstructive surgery.
    Bratisl Lek Listy 2017 ;118(6):370-373
    Background: The aim of the study was to evaluate the impact of cleft lip/palate children together with consequent treatment on quality of family life using standardized questionnaire. Different to previous studies the evaluation of quality of family life by questionnaire was realized twice in the same group of families (before the reconstructive surgery and several months after palatoplasty).

    Methods: The study was conducted in 40 families divided in two groups: 20 families with children with cleft lip (CL), 20 families with children with cleft lip and palate (CLP). Read More

    Association between the heart rate early after heart transplantation and the long term clinical outcomes.
    Bratisl Lek Listy 2017 ;118(6):366-369
    Background: A strong correlation between a lower heart rate and survival has been demonstrated in various patient populations. The optimal heart rate for heart transplant patients is still unknown. The aim of our study was to evaluate the association between an early heart rate and survival after heart transplantation. Read More

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