Search our Database of Scientific Publications and Authors

I’m looking for a

    8664 results match your criteria Bratislavske lekarske listy[Journal]

    1 OF 174

    Quercetin induces apoptosis and necroptosis in MCF-7 breast cancer cells.
    Bratisl Lek Listy 2017 ;118(2):123-128
    Objective: This study investigated the quercetin (Que) effects on growth of MCF-7 human cancer breast cell line and its cellular death mechanism.

    Background: Quercetin has been found to be very efficacious against many different types of cancer cells. However, the study is not sufficiently powered to demonastrate anticancer mechanisms. Read More

    The role of pegaptanib sodium in the suppression of epidural fibrosis in a postlaminectomy rat model.
    Bratisl Lek Listy 2017 ;118(2):118-122
    Objective: Spinal epidural fibrosis is a clinical condition that develops after laminectomy and can compress the spine. Many agents have been tried for the treatment, but none has entered clinical use at present. Pegaptanib sodium is an antiangiogenetic drug that prevents the development of new vessels and thus adhesion by inhibiting the effect of VEGF. Read More

    Role of ischemic modified albumin in the early diagnosis of increased intracranial pressure and brain death.
    Bratisl Lek Listy 2017 ;118(2):112-117
    Aim: Increased intracranial pressure following trauma and subsequent possible development of brain death are important factors for morbidity and mortality due to ischemic changes. We aimed to establish the role of ischemic modified albumin (IMA) in the early diagnosis of the process, starting with increased intracranial pressure and ending with brain death.

    Materials And Methods: Eighteen Wistar-Albino rats were divided into three groups; control (CG, n = 6), increased intracranial pressure (ICPG, n = 6), and brain death (BDG, n = 6). Read More

    Flow cytometric examination of apoptotic effect on brain tissue in postnatal period created by intrauterine oxcarbazepine and gabapentin exposure.
    Bratisl Lek Listy 2017 ;118(2):107-111
    Objective: For epileptics, pregnancy contains the balance between no seizure period and antiepileptic use having the least teratogenicity risk. The purpose is to analyse with flow cytometry the apoptotic effects on postnatal brain tissue caused by prenatal use of second generation antiepileptics oxcarbazepine (OXC) and gabapentin (GBP) having different effect mechanisms.

    Method: 30 (n = 5 each group) Wistar albino male rats (45-days-old) are used. Read More

    The effect of vitamin D on MCF-7 breast cancer cell metabolism.
    Bratisl Lek Listy 2017 ;118(2):101-106
    The role of vitamin D in calcium absorption and bone health is known. The studies revealed that vitamin D modulates breast cancer cell growth and it is also associated with a reduced breast cancer risk. The primary objective of this study was to highlight the metabolic effect of Vitamin D on MCF-7 breast cancer cell line. Read More

    Melatonin: a hepatoprotective agent against radioiodine toxicity in rats.
    Bratisl Lek Listy 2017 ;118(2):95-100
    Objectives: The aim of the current study was to investigate the possible radioprotective effects of melatonin against hepatic radioiodine (RAI) toxicity.

    Methods: Thirty-six rats were randomly divided into three groups: untreated control (Group 1); oral radioiodine (RAI, 111 MBq) administrated rats (Group 2), and melatonin group (oral RAI and daily intraperitoneal injection of 12 mg/kg melatonin-Group 3). In the third group, melatonin administration was started two days before and continued for five days after RAI administration. Read More

    Antioxidant therapy impresses in oxidative stress-induced kidney cells.
    Bratisl Lek Listy 2017 ;118(2):89-94
    Introduction:   Renal cell cancer (RCC) is not a single entity, but consists of different types of tumors derived from various parts of the nephron (epithelium or renal tubules). It is known that cancer initiation and progression is related to the balance between oxidants and antioxidants directly. Interestingly, advanced stages of cancer such as metastasis, angiogenesis are associated with cell oxidative capacity. Read More

    Cisplatin plus norcantharidin alter the expression of TGF-β1/Smads signaling pathway in hepatocellular carcinoma.
    Bratisl Lek Listy 2017 ;118(2):85-88
    Purpose: To investigate the effects of cisplatin plus norcantharidin on transforming growth factor (TGF)-β1/Smads signaling pathway in hepatocellular carcinoma cells.

    Methods: Hepatocellular carcinoma cells (Hep3B) were divided into four groups: control group, cisplatin 2.0 μg/ml group, norcantharidin 10 μg/ml group, and cisplatin 2. Read More

    Effects of sevoflurane on apoptosis, BDNF and cognitive functions in neonatal rats.
    Bratisl Lek Listy 2017 ;118(2):80-84
    Objective: To evaluate the early and late effects of sevoflurane on the neonatal brain.

    Background: Sevoflurane is the most used anaesthetics in neonatal subjects.

    Methods: The study included 7-day-old male Wistar-Albino rats (n = 30), which were divided into the two groups according to the anaesthetic received: sevoflurane (S) and control group (C). Read More

    Plasma nesfatin 1 level in patients with first attack psychosis.
    Bratisl Lek Listy 2017 ;118(2):77-79
    Objectives: This study aimed to investigate the possible relationship between plasma concentrations of nesfatin 1 and first attack psychosis.

    Methods: Totally, 32 patients with the first episode psychosis and 33 randomly selected weight- and body mass index-matched healthy volunteers admitted to Mustafa Kemal University, Faculty of Medicine, Training and Research Hospital, Psychiatry outpatient clinic were included. Healthy control subjects were matched in terms of weight and body mass index (BMI). Read More

    Differentially regulated ADAMTS1, 8, and 18 in gastric adenocarcinoma.
    Bratisl Lek Listy 2017 ;118(2):71-76
    Objective: The aim is to investigate the expression status of ADAMTS1,8, and 18 proteases in gastric cancer (GC) and lymphatic metastasis.

    Background: A disintegrin and metalloprotease with thrombospondin motifs (ADAMTS) is a new protease family, and has important biological functions such as hemostasis, extracellular matrix remodeling and regulation of angiogenesis.

    Methods: The immunostaining status of ADAMTS1,8, and 18 were investigated in formalin-fixed paraffin-embedded samples of 25 patients who underwent curative resection for GC. Read More

    Human race as indicator of 3D planning of soft tissue of face and multidisciplinary approach.
    Bratisl Lek Listy 2017 ;118(7):431-436
    Objective: The aim of this study was to determine the optimal parameters for 3D soft tissue planning for ortognatic treatment by gender and increases the effectiveness of multidisciplinary cooperation.

    Methods: Craniofacial parameters which were analysed: nose breadth (al-al), bi-entocanthion breadth (en-en), bi-zygomatic breadth (zy-zy), bi-gonial breadth (go-go), total facial height (n-gn), mouth breadth (ch-ch), morphologic face height (sn-gn), upper-lip height (Ls-Stm), lower-lip height (Stm-Li) and pupils - mid-face (right). The statistically significant level was determined at p values < 0. Read More

    Risk perception of NSAIDs in hospitalized patients in Greece.
    Bratisl Lek Listy 2017 ;118(7):427-430
    Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used analgesics worldwide in different syndromes. There is a relevant evidence about NSAIDs various adverse effects (AEs) on gastrointestinal, cardiovascular, renal, pulmonary, nervous systems. Many of these problems are preventable with respects to appropriate patient´s risk perception. Read More

    The significance of Akt/NF-κb signaling pathway in the posterior cataract animal model.
    Bratisl Lek Listy 2017 ;118(7):423-426
    Objective: To establish SD rat posterior capsular opacification (posterior capsular opacification- PCO) animal model, and to detect the expression of Akt/NF-kb signaling pathway in the PCO model.

    Methods: 30 healthy SD rats were randomly divided into control group (0d) and the experimental groups (7d and 14 d), there were 10 rats at all time points. All rats (right eye) were treated with the lens capsule, and the inflammatory reaction of the anterior segment of the eye and the occurrence of PCO at different time points were observed under the microscope. Read More

    Effect of different doses of pregabalin on skeletal muscle ischaemia-reperfusion injury in rats.
    Bratisl Lek Listy 2017 ;118(7):417-422
    Aim/introduction: Analgesic, anti-inflammatory and anti-apoptotic effects of pregabalin have been shown previously. In this study, we investigated the protective effect of different doses of pregabalin on skeletal muscle IR injury in rats.

    Materials And Methods: 24 rats were randomly divided into 4 groups (Control, Ischaemia-Reperfusion (IR), IR-Pregabalin 50 mg, IR-Pregabalin 200 mg). Read More

    The impact of sleep deprivation on hippocampal-mediated learning and memory in rats.
    Bratisl Lek Listy 2017 ;118(7):408-416
    Background: To examine the impact of different types of sleep deprivation on hippocampal-mediated learning and memory in rats.

    Methods: Forty-eight Sprague-Dawley male rats were randomly assigned to 1 of 4 equal-size groups: (1) 12 hours of sleep per day (control). (2) total sleep deprivation (TSD), (3) rapid eye movement (REM) deprivation (RD), and (4) sleep restricted to 4 hours per day (SR). Read More

    The impact of using different doses of progesterone on memory performance.
    Bratisl Lek Listy 2017 ;118(7):405-407
    Objective: Progesterone is a sex hormone and its receptors are expressed throughout the hippocampus. This study was aimed at evaluating the effects of different doses of progesterone on memory.

    Methods: Male rats were arbitrarily assigned to nine groups, namely Group I: control, Group II: control-cannula, Group III received 0. Read More

    Effects of granulocyte colony-stimulating factor administration time on pain.
    Bratisl Lek Listy 2017 ;118(7):399-404
    Objective: This study was aimed at evaluating the effect of administration time of granulocyte colony-stimulating factor (G-CSF) on the level of pain related to G-CSF.

    Methods: This study was carried out with 48 cancer patients divided into A and B groups. In the first stage of the study, the groups A and B were planned to be administered with G-CSF at 10: 00 and 14: 00, respectively. Read More

    Roles of adiponectin and leptin as diagnostic markers in pancreatic cancer.
    Bratisl Lek Listy 2017 ;118(7):394-398
    Ntroduction:   Obesity is one of the most serious public health problem worldwide. Adipose tissue synthetize and secrete many growth factors and several cytokines known as adipokines. Studies demonstrated changes in the levels of these adipokines in many types of cancer associated with obesity. Read More

    Effects of ceranib-2 on cell survival and TNF-alpha in colon cancer cell line.
    Bratisl Lek Listy 2017 ;118(7):391-393
    Objective: The aim of this study was to investigate the effects of a novel anti-cancer drug, ceranib-2, which targets the acid ceramidase, in human colon cancer cell line.

    Materials And Methods: The cell lines were treated with 50 µM of ceranib-2. Relative mRNA expression of TNF-alpha, TNF-R1 and ASAH were assessed by quantitative RT-PCR. Read More

    Dose-dependent effects of adiponectin on ADAMTS-9 gene expression in human chondrocytes.
    Bratisl Lek Listy 2017 ;118(7):386-390
    Objective: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), comprising of 19 members is a family of peptidases. They have several vital functions in physiological and pathological processes in organisms. ADAMTS-9 has aggrecanolytic activity and is responsible for degradation of aggrecan mainly in articular cartilage. Read More

    The age at onset in Multiple Sclerosis is associated with patient's prognosis.
    Bratisl Lek Listy 2017 ;118(6):374-377
    Background: The causes of the individual differences in the rate of disability progression in multiple sclerosis (MS) are still not completely clear. According to the long-term prognosis of MS patients, the search for new valuable prognostic markers of "benign" or "malign" MS is necessary.

    Objectives: Our aim was to assess the possible association of MS onset age with the disease disability progression rate in Slovak patients with MS. Read More

    Impact of cleft lip and/or palate in children on family quality of life before and after reconstructive surgery.
    Bratisl Lek Listy 2017 ;118(6):370-373
    Background: The aim of the study was to evaluate the impact of cleft lip/palate children together with consequent treatment on quality of family life using standardized questionnaire. Different to previous studies the evaluation of quality of family life by questionnaire was realized twice in the same group of families (before the reconstructive surgery and several months after palatoplasty).

    Methods: The study was conducted in 40 families divided in two groups: 20 families with children with cleft lip (CL), 20 families with children with cleft lip and palate (CLP). Read More

    Association between the heart rate early after heart transplantation and the long term clinical outcomes.
    Bratisl Lek Listy 2017 ;118(6):366-369
    Background: A strong correlation between a lower heart rate and survival has been demonstrated in various patient populations. The optimal heart rate for heart transplant patients is still unknown. The aim of our study was to evaluate the association between an early heart rate and survival after heart transplantation. Read More

    The influence of food restriction on the small bowel: Does intensive short‑term food restriction lead to weight loss?
    Bratisl Lek Listy 2017 ;118(6):361-365
    The objective of this study was to show morphological changes in the small intestine of Hyplus broiler rabbits following an eight‑day food restriction programme. The control group (C) received food ad libitum (ADL) for the duration of the experiment. Group R1 received 50g of food per day, and group R2 received 65g of food per day. Read More

    Association between pentraxin 3 and growth differentiation factor-15 in adolescent male swimmers.
    Bratisl Lek Listy 2017 ;118(6):355-360
    Background: The purpose of this study was to evaluate plasma pentraxin 3 (PTX3) and growth differentiation factor-15 (GDF-15) concentrations in adolescent male swimmers and compare any possible interactions with canonical biochemical parameters.

    Methods: Twenty-six adolescent male swimmers and 29 gender- and age-matched sedentary controls participated in this study. Fasting blood samples were taken from the participants. Read More

    Therapeutic effects of melatonin and quercetin in improvement of hepatic steatosis in rats through supression of oxidative damage.
    Bratisl Lek Listy 2017 ;118(6):347-354
    Background: Non-alcoholic steatohepatitis, a cause of cirrhosis, is characterized by fatty infiltration of the liver, inflammation, hepatocellular damage and fibrosis. The aim of the present study was to investigate the effects of melatonin and quercetin on CCl4-induced steatosis characterized by fatty infiltration of the liver, inflammation, hepatocellular damage and fibrosis.

    Methods: Rats were divided into 5 groups: Ethanol, Olive oil, CCl4, CCl4+Melatonin (CCl4+Mel), CCl4+Quercetin. Read More

    MAPK pathway regulated the cardiomyocyte apoptosis in mice with post-infarction heart failure.
    Bratisl Lek Listy 2017 ;118(6):339-346
    Background: To explore the role of the MAPK signaling pathway in the cardiomyocyte apoptosis of mice with post-infarction heart failure (HF).

    Methods: Mice were divided into sham and myocardial infarction (MI) groups. Before surgery, the MI group was divided into SB203580 and PBS subgroups. Read More

    Delivery of HIV-1 Nef linked to heat shock protein 27 using a cationic polymer is more effective than cationic lipid in mammalian cells.
    Bratisl Lek Listy 2017 ;118(6):334-338
    Background: Different adjuvants and delivery systems have been used to enhance the potency of DNA vaccines against viral diseases. Among them, heat shock proteins (HSPs) are stress proteins that have multiple roles such as chaperon activity and anti-apoptotic and adjuvant properties. The goal of this study was to compare the expression of HIV-1 Nef, Hsp27 and Hsp27-Nef genes transfected in HEK-293T mammalian cells by TurboFect and Lipofectamine as a cationic polymer and lipid, respectively. Read More

    Risk of anal incontinence in women with inflammatory bowel diseases after delivery.
    Bratisl Lek Listy 2017 ;118(6):328-333
    Aim: The aim of our prospective study was to evaluate the development of postpartum anal incontinence in patients with inflammatory bowel disease (IBD) compared to healthy women.

    Material And Methods: Patients with IBD and healthy controls enrolled in the study from January 1st 2013 to November 30th 2016 and filled in the anal incontinence questionnaire in the beginning of pregnancy and after vaginal delivery. The results were statistically processed using suitable tests. Read More

    The impact of physiological peripartal stress on the lifelong health of newborn.
    Bratisl Lek Listy 2017 ;118(6):324-327
    In the last decades the exponential increase in frequency of Caesarean sections is being observed. At the same time, there is growing amount of epidemiologic data showing higher lifetime risk of incidence of several diseases in offspring who do not experience natural labour. These findings lead to investigation of mechanisms which are responsible for maladaptation of several organ systems. Read More

    Legionella spp. in dental unit waterlines.
    Bratisl Lek Listy 2017 ;118(5):310-314
    Objective: To determine the current presence of Legionella spp. in the output water of dental unit waterlines (DUWLs) and examine its mitigation by disinfection at the Institute of Dentistry and Oral Sciences, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc.

    Material And Methods: The first stage of our survey involved collecting samples of DUWL output water from 50 dental chair units (DCUs), and 2 samples of the incoming potable water. Read More

    A comprehensive guide to telocytes and their great potential in cardiovascular system.
    Bratisl Lek Listy 2017 ;118(5):302-309
    Telocytes, a recently discovered type of interstitial cells, have a very distinctive morphology - the small cell body with long extensions, named telopodes. In our review, apart from introducing general aspects of telocytes, we focus on properties, functions and future potential of those cells in cardiovascular system. However, physiological functions of telocytes in cardiovascular system are still regarded as quite enigmatic. Read More

    Association of the postoperative white blood cells (WBC) count in peripheral blood after radical surgical treatment of left upper lobe non-small cell lung cancer (NSCLC) with overall survival - single center results.
    Bratisl Lek Listy 2017 ;118(5):299-301
    Introduction: The individual patient prognosis after radical surgery for Non-small cell lung cancer (NSCLC) of left upper lobe remains still unclear. The purpose of this study is to evaluate the predictive value of elevated white blood cell count in peripheral blood as early indicator of postoperative NSCLC prognosis.

    Methods: A retrospective statistical analysis was performed studying patients subject to radical treatment of left upper lobe NSCLC in period of five years with subsequent one to three-year monitoring of morbidity and mortality of the patient population. Read More

    Red or white wine consumption effect on atherosclerosis in healthy individuals (In Vino Veritas study).
    Bratisl Lek Listy 2017 ;118(5):292-298
    Aims: Consumption of wine has a protective effect on cardiovascular diseases. Data from prospective, long-term, head-to-head comparisons of effects of different drinks on markers of atherosclerosis have been insufficient.

    Methods And Results: In Vino Veritas (IVV) study is long-term, prospective, multi-centre, randomized trial comparing effects of red and white wine on atherosclerosis. Read More

    Apoptosis of colon cancer cells under the effect of geldanamycin derivate.
    Bratisl Lek Listy 2017 ;118(5):288-291
    Aim: The apoptotic effect of geldanamycin derivative may be important for the colorectal cancer therapy. The mechanisms of apoptosis require understanding of the behavior of colon cancer cell line Colo-205 which mimics colon adenocarcinoma. Therefore, the effect of IC50 dose of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on the colon cancer cells in vitro was studied for its anti-apoptotic activity. Read More

    Which one is more effective for the treatment of rat sepsis model: thalidomide or etanercept?
    Bratisl Lek Listy 2017 ;118(5):283-287
    Objective: We aimed to investigate the protective effect of selected treatment agents on liver injury in lipopolysaccharide (LPS)-induced rat sepsis model.

    Background: The sepsis includes complex inflammatory responses between a microbial pathogen and the host immune system, and leads to organ failure and also death.

    Methods: This study was performed with 29 male Wistar Albino rats. Read More

    Oxidative effects of extremely low frequency magnetic field and radio frequency radiation on testes tissues of diabetic and healthy rats.
    Bratisl Lek Listy 2017 ;118(5):278-282
    With the development of technology, people are increasingly under the exposure of electromagnetic fields. Individuals with chronic diseases such as diabetes are now long-term exposed to Radio Frequency-RF radiation and extremely low frequency (ELF) magnetic fields (MFs). The purpose of this present study is to investigate oxidative effects and antioxidant parameters of ELF MFs and RF radiation on testis tissue in diabetic and healthy rats. Read More

    Protective effect of pravastatin on doxorubicin-induced hepatotoxicity.
    Bratisl Lek Listy 2017 ;118(5):273-277
    Objective: The present study was designed to investigate the possible protection of pravastatin against hepatic oxidative stress and dysfunctions induced by doxorubicin in rats.

    Background: Statins have beneficial effects on oxidative stress and inflammation.

    Methods: Male Sprague-Dawley rats were divided into four groups. Read More

    Anti-oxidative effect of resveratrol on aluminum induced toxicity in rat cerebral tissue.
    Bratisl Lek Listy 2017 ;118(5):269-272
    Introduction: The direct protective effects of resveratrol against oxidative stress have been demonstrated in neuroglial cells, the mechanisms of these effects are not fully understood. The aim of this research was to study the effect of resveratrol on AL induced cerebral injury in rat.

    Methods: We divided the groups as follows with 10 animals each: a) Group I - served as control receiving normal drinking water and diet ad libitum. Read More

    Antiproliferative effect of a food coloring on colon cancer cell line.
    Bratisl Lek Listy 2017 ;118(5):265-268
    4-MEI (4-Methylimidazole) is used as a chemical intermediate, crude material or component in the manufacture of pharmaceuticals, photographic and photothermographic chemicals, dyes and pigments and agricultural chemicals. 4-MEI is unintentionally found in our food. Caramel colour (which is the most used beverage colouring and food), dark beers and common brands of cola drinks may comprise more than 100 μg of this compound per 12-ounce serving. Read More

    Association of angiogenic cytokines (VEGF-A and VEGF-C) and clinical characteristic in women with unexplained recurrent miscarriage.
    Bratisl Lek Listy 2017 ;118(5):258-264
    Introduction: Recurrent miscarriage (RM) defined as 2 or more spontaneous miscarriage before 20 weeks of gestation, affects at least 1 % of couples trying to conceive. In over 50 % of cases, the cause of the loss of pregnancy remains unexplained. Reduced expression of Angiogenic factors such as: vascular endothelial growth factor (VEGF-A) and VEGF-C has been linked with spontaneous miscarriage, likely due to defective foetal and placental angiogenesis. Read More

    Measurement of intact parathormone during operation for primary hyperparathyroidism.
    Bratisl Lek Listy 2017 ;118(5):255-257
    In this prospective study, the role of the intact parathormone (iPHT) levels for the verification of pathologic parathyroid tissue removal during parathyroidectomy, was analyzed in 441 patients diagnosed with primary hyperparathyroidism. The level of intact parathormone was obtained before the initial incision (baseline level) and 10 minutes after the pathologic parathyroid gland removal (control value). In 80 % of cases, the decrease of intact parathormone was more than 50 % of the baseline level. Read More

    A study of clinical, histopathological and direct immunofluorescence diagnosis in pemphigus group Utility of direct immunofluorescence.
    Bratisl Lek Listy 2017 ;118(4):243-249
    Aims: To determine the diagnostic accordance between histopathological and direct immunofluorescence diagnosis of patients with autoimmune vesiculobullous skin diseases.

    Background: The term pemphigus refers to a group of autoimmune blistering diseases mediated by auto-antibodies directed against desmoglein proteins. The differentiation between the various bullous diseases is important for treatment and prognosis. Read More

    Mechanism of grape seeds extract protection against paracetamol renal cortical damage in male Albino rats.
    Bratisl Lek Listy 2017 ;118(4):233-242
    Objective: The aim of the study was to assess the possible protective role of grape seeds extract (GSE) in ameliorating the toxic effects of paracetamol overdose on the rat renal cortical tissue.

    Background: Paracetamol is one of the widely used non-steroidal anti-inflammatory drugs (NSAIDs). Unfortunately, it was reported as the most common cause of toxic ingestion in the world. Read More

    1 OF 174