17,226 results match your criteria Bratislava Medical Journal-bratislavske Lekarske Listy[Journal]


Toxicological testing of a photoactive phthalocyanine-based antimicrobial substance.

Regul Toxicol Pharmacol 2020 May 23:104685. Epub 2020 May 23.

Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

The aim of the study was toxicological testing of an innovative and efficient antimicrobial agent based on photoactive phthalocyanine (Pc) derivative. A promising Aluminium phthalocyanine (AlPc) with efficient and stable antimicrobial effects was subjected to a battery of toxicological tests to avoid local and systemic toxicity hazard. In compliance with the current European legislation restricting the use of experimental animals, the methods comprised exclusively in vitro procedures based on cellular and tissue models of human origin or mimicking human tissues. Read More

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http://dx.doi.org/10.1016/j.yrtph.2020.104685DOI Listing

Urinary proteomics links keratan sulfate degradation and lysosomal enzymes to early type 1 diabetes.

PLoS One 2020 26;15(5):e0233639. Epub 2020 May 26.

Institute of Medical Science, University of Toronto, Toronto, Canada.

Diabetes is the leading cause of end-stage renal disease worldwide. Our understanding of the early kidney response to chronic hyperglycemia remains incomplete. To address this, we first investigated the urinary proteomes of otherwise healthy youths with and without type 1 diabetes and subsequently examined the enriched pathways that might be dysregulated in early disease using systems biology approaches. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233639PLOS

The Absence of PDR16 Gene Restricts the Overexpression of CaSNQ2 Gene in the Presence of Fluconazole in Candida albicans.

Mycopathologia 2020 May 25. Epub 2020 May 25.

Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15, Bratislava 4, Slovak Republic.

In yeast, the PDR16 gene encodes one of the PITP proteins involved in lipid metabolism and is regarded as a factor involved in clinical azole resistance of fungal pathogens. In this study, we prepared Candida albicans CaPDR16/pdr16Δ and Capdr16Δ/Δ heterozygous and homozygous mutant strains and assessed their responses to different stresses. The CaPDR16 deletion strains exhibited increased susceptibility to antifungal azoles and acetic acid. Read More

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http://dx.doi.org/10.1007/s11046-020-00459-4DOI Listing

Atomic-Resolution EDX, HAADF, and EELS Study of GaAsBi Alloys.

Nanoscale Res Lett 2020 May 25;15(1):121. Epub 2020 May 25.

Center for Physical Sciences and Technology, Saulėtekio al. 3, Vilnius, Lithuania.

The distribution of alloyed atoms in semiconductors often deviates from a random distribution which can have significant effects on the properties of the materials. In this study, scanning transmission electron microscopy techniques are employed to analyze the distribution of Bi in several distinctly MBE grown GaAsBi alloys. Statistical quantification of atomic-resolution HAADF images, as well as numerical simulations, are employed to interpret the contrast from Bi-containing columns at atomically abrupt (001) GaAs-GaAsBi interface and the onset of CuPt-type ordering. Read More

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http://dx.doi.org/10.1186/s11671-020-03349-2DOI Listing

Wastewater-based epidemiology to assess the occurrence of new psychoactive substances and alcohol consumption in Slovakia.

Ecotoxicol Environ Saf 2020 May 22;200:110762. Epub 2020 May 22.

Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia. Electronic address:

Consumption of alcohol and new psychoactive substances (NPS) in a population or during special events (music festivals) is usually monitored through individual questionnaires, forensic and toxicological data, and drug seizures. However, consumption estimates have some biases due mostly to the unknown composition of drug pills for NPS and stockpiling for alcohol. The aim of this study was to evaluate for the first time the real use of alcohol and the occurrence of NPS in Slovakia by wastewater-based epidemiology (WBE). Read More

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http://dx.doi.org/10.1016/j.ecoenv.2020.110762DOI Listing

Channel and cut-bluff failure connectivity in a river system: Case study of the braided-wandering Belá River, Western Carpathians, Slovakia.

Sci Total Environ 2020 May 14;733:139409. Epub 2020 May 14.

Institute of Geography, Faculty of Science, UPJŠ in Košice, Jesenná 5, 040 01 Košice, Slovakia.

Straightforward sediment transport is not common in nature and material is storage during transport and reworked by the same processed that lead to initial mass erosion. Despite the development of quantitative assessment by application high accuracy topography measurement, lack of conceptualisation and combination with precise elevation model changes is still missing. This paper presents a field-based channel-bluff connectivity study based on a sediment cascade approach. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139409DOI Listing

Synthesis and mannosidase inhibitory profile of a small library of aminocyclitols from shikimic acid-derived scaffolds.

Carbohydr Res 2020 May 11;493:108027. Epub 2020 May 11.

Department of Glycobiology, Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38, Bratislava, Slovak Republic.

A short synthetic route to a small library of aminocyclitols 14·HCl-19·HCl has been elaborated from the common shikimic acid-derived scaffolds 20 and 21. The developed strategy features three oxidative processes ‒ ozonolysis, dihydroxylation and epoxidation ‒ as the key transformations. The stereochemistry of the newly created stereocentres was confirmed either via crystallographic analysis or by means of NOESY experiments conducted on advanced intermediates. Read More

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http://dx.doi.org/10.1016/j.carres.2020.108027DOI Listing

Shedding Light on the Dark Side of the Microglia.

ASN Neuro 2020 Jan-Dec;12:1759091420925335

Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval.

Microglia, the resident immune cells of the central nervous system, are not a homogeneous population; their morphology, molecular profile, and even their ultrastructure greatly vary from one cell to another. Recent advances in the field of neuroimmunology have helped to demystify the enigma that currently surrounds microglial heterogeneity. Indeed, numerous microglial subtypes have been discovered such as the disease-associated microglia, neurodegenerative phenotype, and Cd11c-positive developmental population. Read More

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http://dx.doi.org/10.1177/1759091420925335DOI Listing

Rho GTPases in Gynecologic Cancers: In-Depth Analysis toward the Paradigm Change from Reactive to Predictive, Preventive, and Personalized Medical Approach Benefiting the Patient and Healthcare.

Cancers (Basel) 2020 May 20;12(5). Epub 2020 May 20.

Predictive, Preventive and Personalised (3P) Medicine, Department of Radiation Oncology, University Hospital Bonn, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany.

Rho guanosine triphospatases (GTPases) resemble a conserved family of GTP-binding proteins regulating actin cytoskeleton dynamics and several signaling pathways central for the cell. Rho GTPases create a so-called Ras-superfamily of GTPases subdivided into subgroups comprising at least 20 members. Rho GTPases play a key regulatory role in gene expression, cell cycle control and proliferation, epithelial cell polarity, cell migration, survival, and apoptosis, among others. Read More

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http://dx.doi.org/10.3390/cancers12051292DOI Listing

Germline Mutation in Resulting in Impaired Protein Stability is Associated with Familial Breast and Thyroid Cancer.

Cancers (Basel) 2020 May 20;12(5). Epub 2020 May 20.

Department of Clinical Genetics, Vejle University Hospital, Institute of Regional Health Research, University of Southern Denmark, 5000 Odense, Denmark.

Multiple primary thyroid cancer (TC) and breast cancer (BC) are commonly diagnosed, and the lifetime risk for these cancers is increased in patients with a positive family history of both TC and BC. Although this phenotype is partially explained by or mutations, a significant number of patients are negative for these alterations. We judiciously recruited patients diagnosed with BC and/or TC having a family history of these tumors and assessed their whole-exome sequencing. Read More

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http://dx.doi.org/10.3390/cancers12051289DOI Listing

Experimental Laboratory Testing on Behavior of Dowels in Concrete Pavements.

Materials (Basel) 2020 May 20;13(10). Epub 2020 May 20.

Transport Infrastructure Department, Transport Research Centre - CDV, 636 00 Brno, Czech Republic.

This paper describes the testing of effectiveness and behavior of dowels placed in transversal joints of concrete pavements, while focusing on dimensions and quality of commonly used materials. The analysis uses experimental tests in laboratory conditions which were performed independently in the Czech Republic and Slovak Republic. The comparison of quality as well as potential use of alternative materials of dowels is made with the use of developed tests focusing on main requirements, such as longitudinal displacement in cement concrete, resistance of coating to damage, and reduced potential to concrete damage. Read More

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http://dx.doi.org/10.3390/ma13102343DOI Listing

Laser-Based Ablation of Titanium-Graphite Composite for Dental Application.

Materials (Basel) 2020 May 18;13(10). Epub 2020 May 18.

Institute of Production Technologies, Faculty of Materials Science and Technology, Slovak University of Technology, J. Bottu 25, 917 24 Trnava, Slovakia.

Biocompatible materials with excellent mechanical properties as well as sophisticated surface morphology and chemistry are required to satisfy the requirements of modern dental implantology. In the study described in this article, an industrial-grade fibre nanosecond laser working at 1064 nm wavelength was used to micromachine a new type of a biocompatible material, Ti-graphite composite prepared by vacuum low-temperature extrusion of hydrogenated-dehydrogenated (HDH) titanium powder mixed with graphite flakes. The effect of the total laser energy delivered to the material per area on the machined surface morphology, roughness, surface element composition and phases transformations was investigated and evaluated by means of scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), confocal laser-scanning microscopy (CLSM) and X-ray diffraction analysis (XRD). Read More

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http://dx.doi.org/10.3390/ma13102312DOI Listing

Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp.

Int J Biol Macromol 2020 May 19. Epub 2020 May 19.

Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovakia. Electronic address:

Complex structure of cyanobacterium Nostoc sp. exopolysaccharide (EPS), with apparent molecular weight 214 × 10 g/mol, can be deduced from its composition. Chemical and NMR analyses found four dominant sugar monomers, namely (1 → 4)-linked α-l-arabinopyranose, β-d-glucopyranose, β-d-xylopyranose and (1 → 3)-linked β-d-mannopyranose, two different uronic acids and a lactyl group, with (1 → 4,6)-linked β-d-glucopyranose as the only branch point suggest a complex structure of this polymer. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.135DOI Listing

Orientation of FtsH protease homologs in Trypanosoma brucei inner mitochondrial membrane and its evolutionary implications.

Mol Biochem Parasitol 2020 May 11;238:111282. Epub 2020 May 11.

Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. Electronic address:

Trypanosoma brucei is an important human pathogen. In this study, we have focused on the characterization of FtsH protease, ATP-dependent membrane-bound mitochondrial enzyme important for regulation of protein abundance. We have determined localization and orientation of all six putative T. Read More

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http://dx.doi.org/10.1016/j.molbiopara.2020.111282DOI Listing

The age-dependent effect of pre-pubertal castration on anxiety-like behaviour in male rats.

Andrologia 2020 May 20:e13649. Epub 2020 May 20.

Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia.

Adolescence is considered to be a critical period of sex hormone action (re)organising the brain and determining the behavioural phenotype. Such organisational effects in the brain might be the cause of sex differences in some behavioural features. In this experiment, we aimed to examine the role of pubertal sex hormones in development of anxiety in male rats. Read More

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http://dx.doi.org/10.1111/and.13649DOI Listing

Is jugular bulb oximetry monitoring associated with outcome in out of hospital cardiac arrest patients?

J Clin Monit Comput 2020 May 20. Epub 2020 May 20.

Department of Anaesthetics, Yeovil Hospital, Greater Kingston, Somerset, BA21 4AT, UK.

Cerebral protection against secondary hypoxic-ischemic brain injury is a key priority area in post-resuscitation intensive care management in survivors of cardiac arrest. Nevertheless, the current understanding of the incidence, diagnosis and its' impact on neurological outcome remains undetermined. The aim of this study was to evaluate jugular bulb oximetry as a potential monitoring modality to detect the incidences of desaturation episodes during post-cardiac arrest intensive care management and to evaluate their subsequent impact on neurological outcome. Read More

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http://dx.doi.org/10.1007/s10877-020-00530-xDOI Listing

Phenolic Compounds from Regulate Viability and Apoptosis of Pancreatic β-Cells Possibly via SERCA Activity.

ACS Med Chem Lett 2020 May 26;11(5):1006-1013. Epub 2020 Mar 26.

Institute of Experimental Pharmacology and Toxicology, Centre of Experimental Medicine SAS, Dubravska cesta 9, 841 01 Bratislava, Slovakia.

The ability of phenolic compounds from to modulate sarco-endoplasmic Ca-ATPase (SERCA1) activity was analyzed. Enzyme activity decrease correlated with the binding energy of agents to SERCA1. Results from theoretical and experimental approaches were coherent in identifying binding sites to SERCA1. Read More

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http://dx.doi.org/10.1021/acsmedchemlett.0c00047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236539PMC

Respiratory Cilia as a Therapeutic Target of Phosphodiesterase Inhibitors.

Front Pharmacol 2020 6;11:609. Epub 2020 May 6.

Department of Pharmacology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.

Mucociliary clearance is an essential airway defense mechanism dependent predominantly on the proper ciliary function and mucus rheology. The crucial role of cilia is evident in `a variety of respiratory diseases, as the ciliary dysfunction is associated with a progressive decline in lung function over time. The activity of cilia is under supervision of multiple physiological regulators, including second messengers. Read More

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http://dx.doi.org/10.3389/fphar.2020.00609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218135PMC

Polysubstituted 5-Phenylazopyrimidines Extremely Fast Non-Ionic Photochromic Oscillators.

Angew Chem Int Ed Engl 2020 May 20. Epub 2020 May 20.

Univerzita Komenskeho v Bratislave, Institute of Chemistry, 84215, Bratislava, SLOVAKIA.

Photochromic systems with ultrahigh rate of thermal relaxation are highly desirable for development of new efficient photochromic oscillators. Based on DFT calculations, we designed a series of 5-phenylazopyrimidines with strong push-pull character in silico and observed very low energy barriers of thermal ( Z )-to-( E ) isomerization. The structure of the ( Z )-isomer of the slowest derivative in the series was confirmed by NMR with in situ irradiation at low temperature. Read More

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http://dx.doi.org/10.1002/anie.202007065DOI Listing

Determination of 5-fluorocytosine, 5-fluorouracil and 5-fluorouridine in hospital wastewater by LC-MS.

J Sep Sci 2020 May 20. Epub 2020 May 20.

Comenius University in Bratislava, Faculty of Natural Sciences, Department of Analytical Chemistry, Ilkovicova 6, Bratislava, SK-842 15, Slovak Republic.

Chemotherapeutics are pharmaceutical compounds whose occurrence in the environment is of growing concern because of the increase in treatments against cancer diseases. They can reach the aquatic ecosystems after passing through wastewater treatment plants without complete removal. One of the most frequently used chemotherapeutics is 5-fluorouracil which exhibits a strong cytostatic effect. Read More

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http://dx.doi.org/10.1002/jssc.202000144DOI Listing

Vegetation affinity of species in the western part of the Carpathians, with as a new association to Slovakia.

Biodivers Data J 2020 4;8:e52151. Epub 2020 May 4.

Department of Vegetation Ecology, Institute of Botany of the Czech Academy of Sciences, Brno, Czech Republic Department of Vegetation Ecology, Institute of Botany of the Czech Academy of Sciences Brno Czech Republic.

(Shuttleworth's bulrush) is a rare species throughout its distribution range including Carpathians. However, a substantial increase in its finds has been noticed in the last twenty years. This study summarises the present knowledge and brings new data on vegetation with occurrence from the western part of the Carpathians (Czech Republic, Slovakia, Poland and Ukraine) with the aims of evaluating the phytosociological affinity of this species and providing new information about the ecology of the relevant plant communities. Read More

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http://dx.doi.org/10.3897/BDJ.8.e52151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217979PMC

Bromelain-based enzymatic debridement as a treatment of choice in high-risk patient with deep facial burns, a case report.

Int J Surg Case Rep 2020 May 11;71:6-10. Epub 2020 May 11.

Teaching Department of Burns and Reconstructive Surgery, Medical Faculty of Comenius University & University Hospital Bratislava, Ruzinovska 6 (Ružinovská), 82101, Bratislava, Slovakia. Electronic address:

Introduction: Deep facial burns are often combined with inhalation injury that could lead to patient destabilization. Accurate timing of surgical debridement of deep burns in a critical patient is the real medical art. Especially in patients with deep burned face and hands, in whom early debridement promises better functional and aesthetic results. Read More

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http://dx.doi.org/10.1016/j.ijscr.2020.04.052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231992PMC

Targeted next generation sequencing of MLH1-deficient, MLH1 promoter hypermethylated and BRAF/RAS-wild-type colorectal adenocarcinomas is effective in detecting tumors with actionable oncogenic gene fusions.

Genes Chromosomes Cancer 2020 May 19. Epub 2020 May 19.

Šikl-s Department of Pathology, Charles University, The Faculty of Medicine and Faculty Hospital in Pilsen, Pilsen, Czech Republic, Pilsen, Czech Republic.

Oncogenic gene fusions represent attractive targets for therapy of cancer. However, the frequency of actionable genomic rearrangements in colorectal cancer (CRC) is very low, and universal screening for these alterations seems to be impractical and costly. To address this problem, several large scale studies retrospectivelly showed that CRC with gene fusions are highly enriched in groups of tumors defined by MLH1 DNA mismatch repair protein deficiency (MLH1d), and hypermethylation of MLH1 promoter (MLH1ph), and/or the presence of microsatellite instability, and BRAF/KRAS wild-type status (BRAFwt/KRASwt). Read More

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http://dx.doi.org/10.1002/gcc.22861DOI Listing

Analytical techniques for multiplex analysis of protein biomarkers.

Expert Rev Proteomics 2020 May 19:1-17. Epub 2020 May 19.

Ephraim Katzir Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel.

: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Read More

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http://dx.doi.org/10.1080/14789450.2020.1763174DOI Listing

Reasons for Facebook Usage: Data From 46 Countries.

Authors:
Marta Kowal Piotr Sorokowski Agnieszka Sorokowska Małgorzata Dobrowolska Katarzyna Pisanski Anna Oleszkiewicz Toivo Aavik Grace Akello Charlotte Alm Naumana Amjad Afifa Anjum Kelly Asao Chiemezie S Atama Derya Atamtürk Duyar Richard Ayebare Mons Bendixen Aicha Bensafia Boris Bizumic Mahmoud Boussena David M Buss Marina Butovskaya Seda Can Katarzyna Cantarero Antonin Carrier Hakan Cetinkaya Daniel Conroy-Beam Marco A C Varella Rosa M Cueto Marcin Czub Daria Dronova Seda Dural Izzet Duyar Berna Ertugrul Agustín Espinosa Ignacio Estevan Carla S Esteves Tomasz Frackowiak Jorge Contreras-Graduño Farida Guemaz Ivana Hromatko Chin-Ming Hui Iskra Herak Jas L Jaafar Feng Jiang Konstantinos Kafetsios Tina Kavcic Leif Edward Ottesen Kennair Nicolas Kervyn Nils C Köbis András Láng Georgina R Lennard Ernesto León Torun Lindholm Giulia Lopez Mohammad Madallh Alhabahba Alvaro Mailhos Zoi Manesi Rocío Martínez Sarah L McKerchar Norbert Meskó Girishwar Misra Hoang Moc Lan Conal Monaghan Emanuel C Mora Alba Moya Garófano Bojan Musil Jean C Natividade George Nizharadze Elisabeth Oberzaucher Mohd S Omar Fauzee Ike E Onyishi Baris Özener Ariela F Pagani Vilmante Pakalniskiene Miriam Parise Farid Pazhoohi Mariia Perun Annette Pisanski Nejc Plohl Camelia Popa Pavol Prokop Muhammad Rizwan Mario Sainz Svjetlana Salkičević Ruta Sargautyte Susanne Schmehl Oksana Senyk Rizwana Shaikh Shivantika Sharad Franco Simonetti Meri Tadinac Truong Thi Khanh Ha Trinh Thi Linh Karina Ugalde González Nguyen Van Luot Christin-Melanie Vauclair Luis D Vega Gyesook Yoo Stanislava Yordanova Stoyanova Zainab F Zadeh Maja Zupančič

Front Psychol 2020 30;11:711. Epub 2020 Apr 30.

University of Ljubljana, Ljubljana, Slovenia.

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http://dx.doi.org/10.3389/fpsyg.2020.00711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204544PMC

Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos.

Med Microbiol Immunol 2020 May 18. Epub 2020 May 18.

Laboratory of Reproductive Physiology, Center of Biosciences, Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Bratislava, Slovak Republic.

It is known that tetraspanin proteins are involved in many physiological somatic cell mechanisms. Additionally, research has indicated they also have a role in various infectious diseases and cancers. This review focuses on the molecular interactions underlying the tetraspanin web formation in gametes. Read More

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http://dx.doi.org/10.1007/s00430-020-00676-0DOI Listing

Mechanical Behavior of Fine Recycled Concrete Aggregate Concrete with the Mineral Admixtures.

Materials (Basel) 2020 May 14;13(10). Epub 2020 May 14.

Department of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Korea.

The paper describes the mechanical behavior of fine recycled concrete aggregate (FRCA) concrete according to the mineral admixtures. Three types of the mineral admixtures, i.e. Read More

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http://dx.doi.org/10.3390/ma13102264DOI Listing

Survey in Wild Boar () Hunted in Tuscany, Central Italy.

Pathogens 2020 May 14;9(5). Epub 2020 May 14.

Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124, Pisa, Italy.

Leptospirosis is a re-emerging, worldwide zoonosis, and wild boar () are involved in its epidemiology as the reservoir. The aim of this study was to investigate the prevalence of with serological, bacteriological, and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. In total, 287 specimens of sera, kidneys, and liver were collected to perform microscopic agglutination tests (MATs), isolation, and RealTime PCR to detect pathogenic ( gene), intermediate ( gene), and saprophytic ( gene) . Read More

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http://dx.doi.org/10.3390/pathogens9050377DOI Listing

Radiocarbon analysis of carbonaceous aerosols in Bratislava, Slovakia.

J Environ Radioact 2020 Jul 16;218:106221. Epub 2020 Mar 16.

Centre for Nuclear and Accelerator Technologies (CENTA), Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia.

Aerosols dispersed in the atmosphere represent important factors influencing not only the environment, but also human health. Carbonaceous aerosols are one of the main components of total atmospheric aerosols, and their sources are of great interest. Radiocarbon analysis provides an excellent way to determine the fraction of fossil and non-fossil aerosols in the atmosphere. Read More

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http://dx.doi.org/10.1016/j.jenvrad.2020.106221DOI Listing

Temporal changes in tritium and radiocarbon concentrations in the western North Pacific Ocean (1993-2012).

J Environ Radioact 2020 Jul 27;218:106238. Epub 2020 Mar 27.

Faculty of Mathematics, Physics and Informatics, Comenius University, 84248, Bratislava, Slovakia.

The western North Pacific is one of the most studied oceanic basins due to its diverse structure and important role in connection with the adjacent reservoirs. Tritium (H) and radiocarbon (C) have been frequently exploited as oceanographic tracers due to their suitable properties; several extensive observation projects, such as GEOSECS, WOCE and WOMARS, used these two radionuclides to investigate different oceanographic processes, pathways, ocean currents and time scales of deep and bottom water formation. Here we evaluate temporal changes in H and C levels in seawater of the western North Pacific Ocean from 1993 to 2012. Read More

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http://dx.doi.org/10.1016/j.jenvrad.2020.106238DOI Listing

Radiocarbon in tree rings from a clean air region in Slovakia.

J Environ Radioact 2020 Jul 17;218:106237. Epub 2020 Apr 17.

Isotope Climatology and Environmental Research Centre, Institute for Nuclear Research, Hungarian Academy of Sciences, 4026 Debrecen, Hungary.

In order to study radiocarbon levels at a clean air location in Slovakia with no significant local anthropogenic effects, we took tree ring samples from a tree in the vicinity of Jasná recreational area in the Low Tatras region in the central part of Slovakia. There are no significant local fossil fuel emission sources, so these samples represent a regional clean air background important for C studies in Slovakia and Central Europe. The growth rings from the sampled tree (European spruce, Picea abies) cover the period from 1911 to 2016. Read More

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http://dx.doi.org/10.1016/j.jenvrad.2020.106237DOI Listing

Efficacy of Pleuran (-Glucan from ) in the Management of Herpes Simplex Virus Type 1 Infection.

Evid Based Complement Alternat Med 2020 13;2020:8562309. Epub 2020 Apr 13.

Department of Paediatrics, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2, 036 59 Martin, Slovakia.

One of the highly prevalent viral pathogens among children and adults causing infection, clinically presenting as herpes labialis, is herpes simplex virus type 1 (HSV-1). The long-term administration of acyclovir, a standard regimen for therapy against HSV-1 infections, can cause viral resistance against this drug. Therefore, the development of natural drugs with low toxicity that are able to enhance host antiviral defense against HSV infection is needed. Read More

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http://dx.doi.org/10.1155/2020/8562309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201855PMC

Night shifts as a learning experience among nursing students across Europe: Findings from a cross-sectional survey.

Nurse Educ Today 2020 Apr 19;90:104441. Epub 2020 Apr 19.

Department of Medical Sciences, University of Udine, Viale Ungheria, 20, 33100 Udine, Italy. Electronic address:

Background: Organising clinical placements during nights has been reported as an additional opportunity to introduce students to the whole picture of their future responsibilities. However, studies in this field are still lacking.

Objectives: To describe and compare (a) the night shift experience of nursing students across Europe, (b) the patient-related problems that they face during night shifts, (c) the night care activities nursing students are called to perform, and (d) the perceived effects of night shift work on learning outcomes. Read More

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http://dx.doi.org/10.1016/j.nedt.2020.104441DOI Listing

Fungal α-amylases from three GH13 subfamilies - Their sequence-structural features and evolutionary relationships.

Int J Biol Macromol 2020 May 13. Epub 2020 May 13.

Department of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, SK-91701 Trnava, Slovakia; Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, SK-84551 Bratislava, Slovakia. Electronic address:

This work delivers in silico analysis of α-amylases of fungal origin from three subfamilies of the α-amylase family GH13. Originally, typical fungal extracellular α-amylases were classified in the subfamily GH13_1, whereas later some intracellular α-amylases from fungi have been placed in GH13_5, which initially covered bacterial liquefying α-amylases. Recently, fungal α-amylases were identified as members of rather actinobacterial subfamily GH13_32. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.069DOI Listing

The problem of accuracy in single-channel open probability measurements.

Prog Biophys Mol Biol 2020 May 13. Epub 2020 May 13.

Department of Cellular Cardiology, Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, Slovakia.

The function of ion channels to mediate the flux of ions through membranes of living cells depends on their number, conductance, and open probability. The open probability, P, characterizes gating of channels that is sensitive to experimental conditions and that can be determined in single-channel experiments. Individual experimental records and even whole series of single-channel activity measurements represent random samples of the stochastic gating continuous in time. Read More

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http://dx.doi.org/10.1016/j.pbiomolbio.2020.05.002DOI Listing

Structural variability of dyads relates to calcium release in rat ventricular myocytes.

Sci Rep 2020 May 15;10(1):8076. Epub 2020 May 15.

Department of Cellular Cardiology, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.

Cardiac excitation-contraction coupling relies on dyads, the intracellular calcium synapses of cardiac myocytes, where the plasma membrane contacts sarcoplasmic reticulum and where electrical excitation triggers calcium release. The morphology of dyads and dynamics of local calcium release vary substantially. To better understand the correspondence between the structure and the functionality of dyads, we estimated incidences of structurally different dyads and of kinetically different calcium release sites and tested their responsiveness to experimental myocardial injury in left ventricular myocytes of rats. Read More

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http://dx.doi.org/10.1038/s41598-020-64840-5DOI Listing

Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition.

Nat Commun 2020 May 15;11(1):2440. Epub 2020 May 15.

Donnelly Centre, University of Toronto, Toronto, ON, Canada.

Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively fused to an intein N-terminal fragment (IN) and C-terminal fragment (IC) derived from a re-engineered split intein GP41-1. The bait/prey binding reconstitutes the intein, which splices the bait and prey peptides into a single intact protein that can be detected by regular protein detection methods such as Western blot analysis and ELISA, serving as readouts of PPIs. Read More

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http://dx.doi.org/10.1038/s41467-020-16299-1DOI Listing

Patients experiences after hospitalization at Gynecologic Obstetrics Department: Identifying parts for improvement.

Ceska Gynekol 2020 ;85(1):59-66

Objective: Evaluation of the quality of the hospital care at individual departments of the clinic from the patient's perspective using a standard questionnaire.

Design: Retrospective observational studies.

Setting: 2nd Department of Obstetrics and Gynecology, University hospital Bratislava, Faculty of Medicine, Comenius University Bratislava, Slovakia. Read More

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January 2020

ReSpect: Relativistic spectroscopy DFT program package.

J Chem Phys 2020 May;152(18):184101

Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, SK-84536 Bratislava, Slovakia.

With the increasing interest in compounds containing heavier elements, the experimental and theoretical community requires computationally efficient approaches capable of simultaneous non-perturbative treatment of relativistic, spin-polarization, and electron correlation effects. The ReSpect program has been designed with this goal in mind and developed to perform relativistic density functional theory (DFT) calculations on molecules and solids at the quasirelativistic two-component (X2C Hamiltonian) and fully relativistic four-component (Dirac-Coulomb Hamiltonian) level of theory, including the effects of spin polarization in open-shell systems at the Kramers-unrestricted self-consistent field level. Through efficient algorithms exploiting time-reversal symmetry, biquaternion algebra, and the locality of atom-centered Gaussian-type orbitals, a significant reduction of the methodological complexity and computational cost has been achieved. Read More

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http://dx.doi.org/10.1063/5.0005094DOI Listing

Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars.

Int J Mol Sci 2020 May 13;21(10). Epub 2020 May 13.

Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dubravska 9, 84505 Bratislava, Slovak Republic.

Bread wheat ( L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Read More

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http://dx.doi.org/10.3390/ijms21103445DOI Listing

Pulmonary Hypertension in Adults with Congenital Heart Disease: Real-World Data from the International COMPERA-CHD Registry.

J Clin Med 2020 05 13;9(5). Epub 2020 May 13.

Universitätsklinik Köln- Herzzentrum, Klinik III für Innere Medizin, 50937 Köln, Germany.

Introduction: Pulmonary hypertension (PH) is a common complication in patients with congenital heart disease (CHD), aggravating the natural, post-operative, or post-interventional course of the underlying anomaly. The various CHDs differ substantially in characteristics, functionality, and clinical outcomes among each other and compared with other diseases with pulmonary hypertension.

Objective: To describe current management strategies and outcomes for adults with PH in relation to different types of CHD based on real-world data. Read More

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http://dx.doi.org/10.3390/jcm9051456DOI Listing

The effects of testosterone on the physiological response to social and somatic stressors.

Psychoneuroendocrinology 2020 Apr 25;117:104693. Epub 2020 Apr 25.

Social, Cognitive and Affective Neuroscience Unit, Department of Cognition, Emotion, and Methods in Psychology, Faculty of Psychology, University of Vienna, Liebiggasse 5, 1010 Vienna, Austria; Department of Behavioural Neuroscience, Institute of Normal and Pathological Physiology, Centre of Experimental Medicine, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia. Electronic address:

Higher testosterone levels in males have previously been linked to decreased stress reactivity, but in other cases, testosterone has been reported to increase the stress response. We addressed these inconsistencies in a placebo-controlled single-dose testosterone administration study, in which 120 male participants were randomly assigned to undergo a cold-pressor test (CPT, a non-social somatic stressor), a socially evaluated cold-pressor test (SECPT, a social-somatic stressor), or a lukewarm water test (LWT, a non-stressful control condition). Throughout the experiment, blood pressure and interbeat intervals were measured continuously, and saliva samples for hormonal analyses were taken repeatedly at predefined time points. Read More

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http://dx.doi.org/10.1016/j.psyneuen.2020.104693DOI Listing

Pulsatile Drug Delivery System Triggered by Acoustic Radiation Force.

Front Bioeng Biotechnol 2020 17;8:317. Epub 2020 Apr 17.

The BioRobotics Institute, Sant'Anna School of Advanced Studies, Pisa, Italy.

Since biological systems exhibit a circadian rhythm (24-hour cycle), they are susceptible to the timing of drug administration. Indeed, several disorders require a therapy that synchronizes with the onset of symptoms. A targeted therapy with spatially and temporally precise controlled drug release can guarantee a considerable gain in terms of efficacy and safety of the treatment compared to traditional pharmacological methods, especially for chronotherapeutic disorders. Read More

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http://dx.doi.org/10.3389/fbioe.2020.00317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202567PMC

Brain Barriers as Targets in Pathologies and Therapy.

Curr Pharm Des 2020 ;26(13):1403-1404

Institute of Neuroimmunology, Slovak Academy of Sciences, Dubravska cesta 9, 84510 Bratislava, Slovakia.

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http://dx.doi.org/10.2174/138161282613200506134601DOI Listing
January 2020

Caffeinated Beverage Intake, Dyspnea With Ticagrelor, and Cardiovascular Outcomes: Insights From the PEGASUS-TIMI 54 Trial.

J Am Heart Assoc 2020 May 15;9(10):e015785. Epub 2020 May 15.

TIMI Study Group Brigham and Women's Hospital Harvard Medical School Boston MA.

Background A proposed cause of dyspnea induced by ticagrelor is an increase in adenosine blood levels. Because caffeine is an adenosine antagonist, it can potentially improve drug tolerability with regard to dyspnea. Furthermore, association between caffeine and cardiovascular events is of clinical interest. Read More

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http://dx.doi.org/10.1161/JAHA.119.015785DOI Listing

Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells.

Antonie Van Leeuwenhoek 2020 May 14. Epub 2020 May 14.

Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, Bratislava, Slovak Republic.

Adhesion of probiotic bacteria to the mucus layer lining the gastrointestinal tract is necessary for its effective colonisation and specific therapeutic effects. Enrichment of growth medium with mucin might stimulate bacterial adhesion, probably by increasing the expression of surface structures responsible for bacteria-gut epithelia and/or mucus interactions. The aim of this study was to determine if pre-cultivation of potentially probiotic strain Lactobacillus reuteri E (LRE) with mucin stimulates its adherence to colon cell line HT-29 and if the increased adhesion modulates mucin expression in these cells. Read More

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http://dx.doi.org/10.1007/s10482-020-01426-1DOI Listing

Ring-Substituted 1-Hydroxynaphthalene-2-Carboxanilides Inhibit Proliferation and Trigger Mitochondria-Mediated Apoptosis.

Int J Mol Sci 2020 May 12;21(10). Epub 2020 May 12.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic.

Ring-substituted 1-hydroxynaphthalene-2-carboxanilides were previously investigated for their antimycobacterial properties. In our study, we have shown their antiproliferative and cell death-inducing effects in cancer cell lines. Cell proliferation and viability were assessed by WST-1 assay and a dye exclusion test, respectively. Read More

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http://dx.doi.org/10.3390/ijms21103416DOI Listing

Psychotic experiences in student population during the COVID-19 pandemic.

Schizophr Res 2020 May 13. Epub 2020 May 13.

The Centre for Psychiatric Disorders Research, Science Park, Comenius University in Bratislava, Bratislava, Slovak Republic.

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http://dx.doi.org/10.1016/j.schres.2020.05.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218396PMC