15,863 results match your criteria Bratislava Medical Journal-bratislavske Lekarske Listy[Journal]


On Histologic Variability of HPV-associated Endocervical Adenocarcinomas.

Am J Surg Pathol 2019 Apr 15. Epub 2019 Apr 15.

Department of Pathology, University of British Columbia, Royal Columbian Hospital, Vancouver, BC, Canada.

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http://dx.doi.org/10.1097/PAS.0000000000001259DOI Listing

Self-Assembly Properties of Cationic Gemini Surfactants with Biodegradable Groups in the Spacer.

Molecules 2019 Apr 15;24(8). Epub 2019 Apr 15.

Faculty of Pharmacy, Comenius University, Odbojárov 10, 83232 Bratislava, Slovakia.

Self-assembly properties of cationic gemini surfactants with biodegradable amide or ester groups in the spacer were investigated utilising time-resolved fluorescence quenching, dynamic light scattering and zeta potential measurements. A correlation between aggregation parameters such as micelle aggregation number, micelle size and zeta potential with the structure of gemini molecules was made. For gemini molecules with medium spacer lengths, micelle aggregation number does not change much with the surfactant concentration. Read More

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http://dx.doi.org/10.3390/molecules24081481DOI Listing

Grina/TMBIM3 modulates voltage-gated Ca2.2 Ca channels in a G-protein-like manner.

Cell Calcium 2019 Apr 8;80:71-78. Epub 2019 Apr 8.

Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Fakultät für Medizin, Albert-Ludwigs-Universität Freiburg, Albertstr. 25, 79104 Freiburg, Germany. Electronic address:

Grina/TMBIM3 is a poorly characterized transmembrane protein with a broad expression pattern in mammals and with a very ancient origin within eukaryotes. Although initially characterized as an NMDA-receptor associated subunit, there is increasing evidence that Grina/TMBIM3 is involved in the unfolded protein response and controls apoptosis via regulation of Ca homeostasis. Here, we investigate a putative direct interaction of Grina/TMBIM3 with voltage gated Ca channels, in particular with the Ca2. Read More

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http://dx.doi.org/10.1016/j.ceca.2019.04.002DOI Listing

Linking past uses of legacy SVOCs with today's indoor levels and human exposure.

Environ Int 2019 Apr 13;127:653-663. Epub 2019 Apr 13.

Department of Environmental Medicine, Faculty of Public Health, Slovak Medical University, Limbová 12, 83303 Bratislava, Slovakia.

Semivolatile organic compounds (SVOCs) emitted from consumer products, building materials, and indoor and outdoor activities can be highly persistent in indoor environments. Human exposure to and environmental contamination with polychlorinated biphenyls (PCBs) was previously reported in a region near a former PCB production facility in Slovakia. However, we found that the indoor residential PCB levels did not correlate with the distance from the facility. Read More

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http://dx.doi.org/10.1016/j.envint.2019.04.001DOI Listing
April 2019
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Forensic classification of black inkjet prints using Fourier transform near-infrared spectroscopy and Linear Discriminant Analysis.

Forensic Sci Int 2019 Mar 30;299:128-134. Epub 2019 Mar 30.

Leopold-Franzens University, Institute of Analytical Chemistry and Radiochemistry, CCB - Center for Chemistry and Biomedicine, Innrain 80/82, 6020 Innsbruck, Austria. Electronic address:

This work presents a study regarding the forensic discrimination of black inkjet-printed documents in question. Nondestructive Fourier transform near-infrared (FT-NIR) spectroscopy in combination with supervised classification method Discriminant Analysis (DA); Linear Discriminant Analysis (LDA) and Quadratic Discriminant Analysis (QDA) were utilized to investigate 22 different prints of the three most sold office printer brands. The spectra were acquired using the FT-NIR spectrometer NIRFlex N-500 (Büchi Labortechnik AG, Flawil, Switzerland) with the Fiber Optic Solids measuring cell in the spectral region of 10,000-4000 cm. Read More

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http://dx.doi.org/10.1016/j.forsciint.2019.03.041DOI Listing

Quantum non-demolition measurement of an electron spin qubit.

Nat Nanotechnol 2019 Apr 15. Epub 2019 Apr 15.

Center for Emergent Matter Science, RIKEN, Wako-shi, Saitama, Japan.

Measurements of quantum systems inevitably involve disturbance in various forms. Within the limits imposed by quantum mechanics, there exists an ideal projective measurement that does not introduce a back action on the measured observable, known as a quantum non-demolition (QND) measurement. Here we demonstrate an all-electrical QND measurement of a single electron spin in a gate-defined quantum dot. Read More

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http://dx.doi.org/10.1038/s41565-019-0426-xDOI Listing

On the Formation of Nanocrystalline Grains in Metallic Glasses by Means of In-Situ Nuclear Forward Scattering of Synchrotron Radiation.

Nanomaterials (Basel) 2019 Apr 4;9(4). Epub 2019 Apr 4.

Institute of Nuclear and Physical Engineering, Faculty of Electrical Engineering and Information Technology, Slovak University of Technology in Bratislava, Ilkovičova 3, 812 19 Bratislava, Slovakia.

Application of the so-called nuclear forward scattering (NFS) of synchrotron radiation is presented for the study of crystallization of metallic glasses. In this process, nanocrystalline alloys are formed. Using NFS, the transformation process can be directly observed during in-situ temperature experiments not only from the structural point of view, i. Read More

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http://dx.doi.org/10.3390/nano9040544DOI Listing

Dandelion is more tolerant to cadmium than to nickel excess.

Chemosphere 2019 Jun 27;224:884-891. Epub 2019 Feb 27.

Department of Pharmacognosy, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria.

Comparative accumulation of cadmium (Cd) and nickel (Ni) and the consequences for the metabolism of common weed dandelion (triploid ones of Taraxacum sect. Taraxacum) were studied here for the first time. Cd accumulated more in both shoots and roots (489 and 2486 μg/g DW) than Ni (165 and 858 μg/g DW) after 14 days of exposure and only root Ni content did not increase between 7 and 14 days of exposure. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00456535193040
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http://dx.doi.org/10.1016/j.chemosphere.2019.02.181DOI Listing
June 2019
1 Read

Neuroimaging biomarkers for clinical trials in atypical parkinsonian disorders: Proposal for a Neuroimaging Biomarker Utility System.

Alzheimers Dement (Amst) 2019 Dec 2;11:301-309. Epub 2019 Apr 2.

Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom.

Introduction: Therapeutic strategies targeting protein aggregations are ready for clinical trials in atypical parkinsonian disorders. Therefore, there is an urgent need for neuroimaging biomarkers to help with the early detection of neurodegenerative processes, the early differentiation of the underlying pathology, and the objective assessment of disease progression. However, there currently is not yet a consensus in the field on how to describe utility of biomarkers for clinical trials in atypical parkinsonian disorders. Read More

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http://dx.doi.org/10.1016/j.dadm.2019.01.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446052PMC
December 2019

Vaginal dryness: individualised patient profiles, risks and mitigating measures.

EPMA J 2019 Mar 2;10(1):73-79. Epub 2019 Mar 2.

9Radiological clinic, UKB, Rheinische Friedrich-Wilhelms-University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.

Vaginal dryness (VD) affects both pre- and postmenopausal women at any age. Since the hormonal regulation changes during the climacteric period are considered as being the main course of the VD, affected women prefer not to talk about the problem. However, the problem does exist, and unfortunately if any, relatively minor group in the population possesses the health literacy at sufficient level to understand that VD is a suboptimal health condition which carries a multi-factorial character. Read More

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http://link.springer.com/10.1007/s13167-019-00164-3
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http://dx.doi.org/10.1007/s13167-019-00164-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459457PMC
March 2019
5 Reads

A decline in molluscan carbonate production driven by the loss of vegetated habitats encoded in the Holocene sedimentary record of the Gulf of Trieste.

Sedimentology 2019 Apr 25;66(3):781-807. Epub 2018 Aug 25.

Department of Palaeontology University of Vienna Althanstrasse 14 Vienna 1090 Austria.

Carbonate sediments in non-vegetated habitats on the north-east Adriatic shelf are dominated by shells of molluscs. However, the rate of carbonate molluscan production prior to the 20th century eutrophication and overfishing on this and other shelves remains unknown because: (i) monitoring of ecosystems prior to the 20th century was scarce; and (ii) ecosystem history inferred from cores is masked by condensation and mixing. Here, based on geochronological dating of four bivalve species, carbonate production during the Holocene is assessed in the Gulf of Trieste, where algal and seagrass habitats underwent a major decline during the 20th century. Read More

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http://dx.doi.org/10.1111/sed.12516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446828PMC

Promotion of whole blood rheology after vitamin C supplementation: Focus on red blood cells.

Can J Physiol Pharmacol 2019 Apr 13. Epub 2019 Apr 13.

Center of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia ;

Hemorheological properties represent significant contributors in the pathogenesis of cardiovascular diseases. As plasma vitamin C is inversely associated with blood viscosity in humans, we aimed to characterize the effect of vitamin C supplementation on hemorheology with an emphasis on erythrocyte functions. Twenty young healthy volunteers were ask to take vitamin C (1000 mg/day) for three weeks. Read More

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http://www.nrcresearchpress.com/doi/10.1139/cjpp-2018-0735
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http://dx.doi.org/10.1139/cjpp-2018-0735DOI Listing
April 2019
1 Read

Fluorescence measurements of peroxynitrite/peroxynitrous acid in cold air plasma treated aqueous solutions.

Phys Chem Chem Phys 2019 Apr 15. Epub 2019 Apr 15.

Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina, 84248 Bratislava, Slovakia.

Qualitative detection of peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) as one of the key bactericidal agents produced in cold air plasma activated aqueous solutions is presented. We examined the use of the 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescent dye to detect ONOO-/ONOOH in plasma activated non-buffered water (PAW) or buffered solution (PAPB) generated by DC-driven self-pulsed transient spark discharge at atmospheric pressure in ambient air. The diagnostic selectivity of H2DCFDA to reactive oxygen and nitrogen species (RONS) typical of plasma activated aqueous solutions was examined by using various scavengers of RONS. Read More

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http://dx.doi.org/10.1039/c9cp00871cDOI Listing

Hydrogen-rich water reduces liver fat accumulation and improves liver enzyme profiles in patients with non-alcoholic fatty liver disease: a randomized controlled pilot trial.

Clin Res Hepatol Gastroenterol 2019 Apr 11. Epub 2019 Apr 11.

Applied Bioenergetics Lab, Faculty of Sport and PE, University of Novi Sad, Novi Sad, Serbia; Faculty of Health Sciences, University of Pécs, Pécs, Hungary. Electronic address:

Background And Aims: While non-alcoholic fatty liver disease (NAFLD) is rapidly becoming the most common liver disease worldwide, its treatment remains elusive. Since metabolic impairment plays a major role in NAFLD pathogenesis, any pharmaceuticals, such as molecular hydrogen (H), that advance lipid and glucose metabolism could be appropriate to tackle this complex condition. The aim of this study was to analyze the effects of 28-day hydrogen-rich water intake on liver fat deposition, body composition and lab chemistry profiles in overweight patients suffering from mild-to-moderate NAFLD. Read More

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http://dx.doi.org/10.1016/j.clinre.2019.03.008DOI Listing

The role of the protein-RNA recognition code in neurodegeneration.

Authors:
Jozef Nahalka

Cell Mol Life Sci 2019 Apr 12. Epub 2019 Apr 12.

Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska cesta 9, 84538, Bratislava, Slovak Republic.

MicroRNAs are small endogenous RNAs that pair and bind to sites on mRNAs to direct post-transcriptional repression. However, there is a possibility that microRNAs directly influence protein structure and activity, and this influence can be termed post-translational riboregulation. This conceptual review explores the literature on neurodegenerative disorders. Read More

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http://dx.doi.org/10.1007/s00018-019-03096-3DOI Listing
April 2019
2 Reads

Developmental Dysplasia of Hip: Perspectives in Genetic Screening.

Med Sci (Basel) 2019 Apr 11;7(4). Epub 2019 Apr 11.

Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia.

Development dysplasia of the hip (DDH) is a complex developmental disorder despite being a relatively common condition mainly caused by incompatibility of the femoral head and the abnormal joint socket. Development dysplasia of the hip describes a wide spectrum of disorders ranging from minor acetabular dysplasia to irreducible dislocation of the hip. Modern medicine still suffers from lack of information about screening and precise genetic examination. Read More

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http://dx.doi.org/10.3390/medsci7040059DOI Listing
April 2019
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Eye hazard classification according to UN GHS / EU CLP and the severity of eye symptoms caused by accidental exposures to detergents and cleaning products.

Regul Toxicol Pharmacol 2019 Apr 9. Epub 2019 Apr 9.

Toxicological Information Centre, Department of Occupational Medicine, First Faculty of Medicine, Charles University and General University Hospital Prague, Czech Republic.

The use of lower cut-off values/concentration limits for the calculation of mixture classification in UN GHS/EU CLP versus the previous regulatory scheme (EU Dangerous Preparations Directive, DPD), has resulted in an increased number of classifications in the highest eye hazard category. Herein, a semi-quantitative categorisation of severity of eye effects, following accidental human exposures to detergents, was compared to the classification category of the products. Three schemes were evaluated: EU DPD; EU CLP (based on all available data and information, including weight of evidence); and EU CLP (based entirely on the calculation method). Read More

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http://dx.doi.org/10.1016/j.yrtph.2019.04.004DOI Listing

Flooding and hydrologic connectivity modulate community assembly in a dynamic river-floodplain ecosystem.

PLoS One 2019 12;14(4):e0213227. Epub 2019 Apr 12.

Austrian Science Fund FWF, Vienna, Austria.

Braided river floodplains are highly dynamic ecosystems, where aquatic communities are strongly regulated by the hydrologic regime. So far, however, understanding of how flow variation influences assembly mechanisms remains limited. We collected benthic chironomids and oligochaetes over a year across a lateral connectivity gradient in the semi-natural Tagliamento River (Italy). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0213227PLOS

Genome Sequence of an Arthroconidial Yeast, Saprochaete fungicola CBS 625.85.

Microbiol Resour Announc 2019 Apr 11;8(15). Epub 2019 Apr 11.

Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova, Bratislava, Slovak Republic

is an arthroconidial yeast classified in the / clade of the subphylum Saccharomycotina. Here, we report the genome sequence of holotype strain CBS 625.85, assembled to five putative chromosomes. Read More

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http://genomea.asm.org/lookup/doi/10.1128/MRA.00092-19
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http://dx.doi.org/10.1128/MRA.00092-19DOI Listing
April 2019
1 Read

Profiling continuous sleep representations for better understanding of the dynamic character of normal sleep.

Artif Intell Med 2018 Dec 29. Epub 2018 Dec 29.

Institute of Measurement Science, Slovak Academy of Sciences, Bratislava, Slovakia. Electronic address:

The amount and quality of sleep substantially influences health, daily behaviour and overall quality of life. The main goal of this study was to investigate to what extent sleep structure, as derived from the polysomnographic (PSG) recordings of nocturnal human sleep, can provide information about sleep quality in terms of correlating with a set of variables representing the daytime subjective, neurophysiological and cognitive states of a healthy population without serious sleep problems. We focused on a continuous sleep representation derived from the probabilistic sleep model (PSM), which describes the microstructure of sleep by a set of sleep probabilistic curves representing a finite number of sleep microstates. Read More

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http://dx.doi.org/10.1016/j.artmed.2018.12.009DOI Listing
December 2018

Lung Eosinophils - a Novel 'Virus Sink' that is Defective in Asthma?

Allergy 2019 Apr 10. Epub 2019 Apr 10.

Child Life and Health and Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom.

Eosinophils represent one of the most studied cells in the development and pathophysiology of various immune mediated disorders and conditions. Moreover, they serve as important biomarkers (diagnostic, therapy-driving, prognostic) in daily allergy praxis and can be affected by several therapeutic interventions. Eosinophils differentiate from a pluripotent progenitor under the influence of several cytokines and growth factors. Read More

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http://dx.doi.org/10.1111/all.13811DOI Listing
April 2019
5 Reads

Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases.

Nat Commun 2019 04 10;10(1):1669. Epub 2019 Apr 10.

School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.

Fungal cell wall synthesis is achieved by a balance of glycosyltransferase, hydrolase and transglycosylase activities. Transglycosylases strengthen the cell wall by forming a rigid network of crosslinks through mechanisms that remain to be explored. Here we study the function of the Aspergillus fumigatus family of five Crh transglycosylases. Read More

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http://dx.doi.org/10.1038/s41467-019-09674-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458159PMC

Quality evaluation of sweet potato powder-enriched cereal products.

Food Sci Technol Int 2019 Apr 10:1082013219842711. Epub 2019 Apr 10.

Department of Food Technology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.

The effects of wheat flour substitution by sweet potato powder on rheological parameters of wheat dough and physical parameters of cookies and crackers were analyzed. Wheat flour was substituted with sweet potato powder at level 2.5%, 5%, 7. Read More

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http://dx.doi.org/10.1177/1082013219842711DOI Listing

Anticancer Activities of L. in Experimental Breast Carcinoma in Vivo and in Vitro.

Int J Mol Sci 2019 Apr 9;20(7). Epub 2019 Apr 9.

Weill Cornell Medicine in Qatar, Qatar Foundation-Education City, Doha 24144, Qatar.

Naturally-occurring mixtures of phytochemicals present in plant foods are proposed to possess tumor-suppressive activities. In this work, we aimed to evaluate the antitumor effects of L. in in vivo and in vitro mammary carcinoma models. Read More

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http://dx.doi.org/10.3390/ijms20071749DOI Listing
April 2019
2.862 Impact Factor

Two-generational effects of Benzophenone-3 on the aquatic midge Chironomus riparius.

Sci Total Environ 2019 Jun 3;669:983-990. Epub 2019 Mar 3.

Departamento de Biologia & CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

Organic UV-filters are emergent contaminants continuously released into the aquatic ecosystems. These compounds are persistent showing potential for bioaccumulation. Partial life-cycle tests may underestimate the toxicity of UV-filters especially since these compounds have shown to act as endocrine disruptors. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.03.023DOI Listing

Hydrogen gas: from clinical medicine to an emerging ergogenic molecule for sports athletes.

Can J Physiol Pharmacol 2019 Apr 10. Epub 2019 Apr 10.

Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia, , Dubravska 9 , Bratislava, Slovakia , 841 0 ;

H2 has been clinically demonstrated to provide anti-oxidant and anti-inflammatory effects, which makes it an attractive agent in exercise medicine. Although exercise provides a multiplicity of benefits including decreased risk of disease, it can also have detrimental effects. For example, chronic high-intensity exercise in elite athletes, or sporadic bouts of exercise (i. Read More

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http://dx.doi.org/10.1139/cjpp-2019-0067DOI Listing
April 2019
1 Read

From Allostatic Load to Allostatic State-An Endogenous Sympathetic Strategy to Deal With Chronic Anxiety and Stress?

Front Behav Neurosci 2019 21;13:47. Epub 2019 Mar 21.

School of Medical Biology, South Ural State University, Chelyabinsk, Russia.

The concepts of allostatic load and overload, i. e., a dramatic increase in the allostatic load that predisposes to disease, have been extensively described in the literature. Read More

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http://dx.doi.org/10.3389/fnbeh.2019.00047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442703PMC
March 2019
4.160 Impact Factor

Use of Irradiated Polymers after Their Lifetime Period.

Polymers (Basel) 2018 Jun 9;10(6). Epub 2018 Jun 9.

Faculty of Applied Informatics, Tomas Bata University in Zlin, CEBIA-Tech, Nad Stranemi 4511, 760 05 Zlin, Czech Republic.

This article deals with the study of the utilisation of irradiated HDPE products after their end-of-life cycle. Today, polymer waste processing is a matter of evermore intensive discussion. Common thermoplastic waste recycling-especially in the case of wastes with a defined composition-is generally well-known-and frequently used. Read More

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http://dx.doi.org/10.3390/polym10060641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404028PMC

Functionalization with a VEGFR2-binding antibody fragment leads to enhanced endothelialization of a cardiovascular stent in vitro and in vivo.

J Biomed Mater Res B Appl Biomater 2019 Apr 9. Epub 2019 Apr 9.

Centre for Research in Medical Devices (CÚRAM), NUI Galway, Galway, Ireland.

Rapid endothelialization of cardiovascular stents is critical to prevent major clinical complications such as restenosis. Reconstruction of the native endothelium on the stent surface can be achieved by the capture of endothelial progenitor cells (EPCs) or neighboring endothelial cells (ECs) in vivo. In this study, stainless steel cardiovascular stents were functionalized with recombinant scFv antibody fragments specific for vascular endothelial growth factor receptor-2 (VEGFR2) that is expressed on EPCs and ECs. Read More

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http://dx.doi.org/10.1002/jbm.b.34380DOI Listing
April 2019
1 Read

Is neurogenesis in two songbird species related to their song sequence variability?

Proc Biol Sci 2019 Jan;286(1895):20182872

Centre of Biosciences, Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences , Bratislava , Slovakia.

Neurogenesis takes part in the adult songbird brain and new neurons are integrated into the forebrain including defined areas involved in the control of song learning and production. It has been suggested that the new neurons in the song system might enable vocal variability. Here, we examined the basal levels of neurogenesis in two songbird species, zebra finch ( Taeniopygia guttata) and Bengalese finch ( Lonchura striata var. Read More

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http://dx.doi.org/10.1098/rspb.2018.2872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364589PMC
January 2019

Clinical study of effectiveness and safety of CELcomplex containing Cucurbita Pepo Seed extract and Flax and Casuarina on stress urinary incontinence in women.

J Tradit Complement Med 2019 Apr 3;9(2):138-142. Epub 2018 May 3.

Department of Pharmacology and Toxicology, Faculty of Pharmacy in Bratislava, Comenius University, Slovakia.

Aim: The safety and effectiveness of a preparation containing a mix of Cucurbita Pepo Seed extract, Equisetum arvense and Linum usitatissimum - Flax A (CELcomplex) on stress urinary incontinence (SUI) was evaluated in female patients recruited from 20 urological and gynaecological outpatient clinics in Slovakia.

Methods: A total of 86 women aged from 32 to 88 with SUI (grade 1 = 44, grade 2 = 42) were enrolled in the study and followed-up for six weeks (point 1) and twelve weeks (point 2). The primary outcome of the study was evaluated by changes in day-time and nocturnal urinary frequency (bathroom visits) and urinary incontinence episodes (leaks). Read More

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http://dx.doi.org/10.1016/j.jtcme.2017.10.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435946PMC

Identification of a molecular gating determinant within the carboxy terminal region of Ca3.3 T-type channels.

Mol Brain 2019 04 8;12(1):34. Epub 2019 Apr 8.

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague, Czech Republic.

The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Read More

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http://dx.doi.org/10.1186/s13041-019-0457-0DOI Listing

Neurocognitive Predictors of Understanding of Intentions in Parkinson Disease.

J Geriatr Psychiatry Neurol 2019 Apr 8:891988719841727. Epub 2019 Apr 8.

2 Department of Psychiatry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.

Objectives: Theory of Mind (ToM), the ability to understand other people's mental states, is essential in everyday social interactions. The relationship between cognitive domains and ToM impairment in Parkinson disease (PD) has been receiving growing attention with ambiguous findings. The objective of the current study was to ascertain which cognitive domain predicts understanding of intentions and the impact of PD-specific clinical measures on ToM performance. Read More

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http://dx.doi.org/10.1177/0891988719841727DOI Listing
April 2019
2 Reads

Block Copolymer of Flexible and Semi-Flexible Block Confined in Nanopost Array.

Polymers (Basel) 2018 Nov 23;10(12). Epub 2018 Nov 23.

Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.

Coarse-grained molecular dynamics simulations of a diblock copolymer consisting of a flexible and semi-flexible block in a dense array of parallel nanoposts with a square lattice packing were performed. The mutual interactions between the two blocks of the confined diblock chain were investigated through a comparison of their size, structure, and penetration among nanoposts with the corresponding separate chains. The geometry of a nanopost array was varied at constant post separation or at constant width of the passage between nanoposts. Read More

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http://dx.doi.org/10.3390/polym10121301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401765PMC
November 2018

Chromatin Loop Extrusion and Chromatin Unknotting.

Polymers (Basel) 2018 Oct 11;10(10). Epub 2018 Oct 11.

Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland.

It has been a puzzle how decondensed interphase chromosomes remain essentially unknotted. The natural expectation is that in the presence of type II DNA topoisomerases that permit passages of double-stranded DNA regions through each other, all chromosomes should reach the state of topological equilibrium. The topological equilibrium in highly crowded interphase chromosomes forming chromosome territories would result in formation of highly knotted chromatin fibres. Read More

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http://dx.doi.org/10.3390/polym10101126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403842PMC
October 2018

Light-Induced Actuation of Poly(dimethylsiloxane) Filled with Graphene Oxide Grafted with Poly(2-(trimethylsilyloxy)ethyl Methacrylate).

Polymers (Basel) 2018 Sep 24;10(10). Epub 2018 Sep 24.

Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, 845 41 Bratislava, Slovakia.

This study serves to combine two approaches into one single step, to achieve a significant improvement of the light-induced actuation capabilities. Graphene oxide (GO) is an inert material, from the electrical and thermal conductivity point of view, and is incompatible with the usually-used poly(dimethylsiloxane) (PDMS) matrix. During surface-modification by surface-initiated atom transfer radical polymerization, the GO was transformed into a conducting and compatible material with the PDMS showing enormous light-induced actuation capability. Read More

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http://dx.doi.org/10.3390/polym10101059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403827PMC
September 2018

PDDA-Montmorillonite Composites Loaded with Ru Nanoparticles: Synthesis, Characterization, and Catalytic Properties in Hydrogenation of 2-Butanone.

Polymers (Basel) 2018 Aug 4;10(8). Epub 2018 Aug 4.

Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 36 Bratislava, Slovakia.

The effect of synthesis parameters on the physicochemical properties of clay/ polydiallyldimethylammonium (PDDA)/Ru composites and their applicability in hydrogenation of 2-butanone under very mild conditions (room temperature, atmospheric pressure, and aqueous solution) was studied. Three synthetic procedures were employed, differing in the order of addition of components and the stage at which metallic Ru species were generated. The materials were characterized with XRD (X-ray diffraction), XRF (X-ray fluorescence), EDS (energy-dispersive spectroscopy), AFM (atomic force microscopy), TEM/HRTEM (transmission electron microscopy/high resolution transmission electron microscopy), and TG/DSC (thermal gravimetry/differential scanning microscopy techniques. Read More

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http://dx.doi.org/10.3390/polym10080865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403927PMC

Reversible Actuation Ability upon Light Stimulation of the Smart Systems with Controllably Grafted Graphene Oxide with Poly (Glycidyl Methacrylate) and PDMS Elastomer: Effect of Compatibility and Graphene Oxide Reduction on the Photo-Actuation Performance.

Polymers (Basel) 2018 Jul 28;10(8). Epub 2018 Jul 28.

Polymer Institute, Slovak Academy of Sciences, Dúbravska cesta 9, 845 41 Bratislava, Slovakia.

This study is focused on the controllable reduction of the graphene oxide (GO) during the surface-initiated atom transfer radical polymerization technique of glycidyl methacrylate (GMA). The successful modification was confirmed using TGA-FTIR analysis and TEM microscopy observation of the polymer shell. The simultaneous reduction of the GO particles was confirmed indirectly via TGA and directly via Raman spectroscopy and electrical conductivity investigations. Read More

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http://dx.doi.org/10.3390/polym10080832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403919PMC

Electrospinning of Ethylene Vinyl Acetate/Carbon Nanotube Nanocomposite Fibers.

Polymers (Basel) 2019 Mar 22;11(3). Epub 2019 Mar 22.

Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.

Nanocomposites, based on an ethylene vinyl acetate (EVA) copolymer with a vinyl acetate content of 34 wt % and varying amounts of multiwall carbon nanotubes (MWCNTs), were prepared by an electrospinning method. The dispersibility of the MWCNTs in the solution was improved by using cholesteryl 1-pyrenecarboxylate (PyChol) as a compatibilizer. The transmission electron microscopy images showed that the MWCNTs were aligned inside of the elastomeric matrix by the electrospinning process. Read More

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http://dx.doi.org/10.3390/polym11030550DOI Listing

Inorganic Polysulfides and Related Reactive Sulfur–Selenium Species from the Perspective of Chemistry.

Molecules 2019 04 6;24(7). Epub 2019 Apr 6.

Division of Bioorganic Chemistry, School of Pharmacy, University of Saarland, D-66123 Saarbruecken, Germany.

Polysulfides (H₂S) represent a class of reactive sulfur species (RSS) which includes molecules such as H₂S₂, H₂S₃, H₂S₄, and H₂S and whose presence and impact in biological systems, when compared to other sulfur compounds, has only recently attracted the wider attention of researchers. Studies in this field have revealed a facet-rich chemistry and biological activity associated with such chemically simple, still unusual inorganic molecules. Despite their chemical simplicity, these inorganic species, as reductants and oxidants, metal binders, surfactant-like "cork screws" for membranes, components of perthiol signalling and reservoirs for inorganic hydrogen sulfide (H₂S), are at the centre of complicated formation and transformation pathways which affect numerous cellular processes. Read More

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http://dx.doi.org/10.3390/molecules24071359DOI Listing
April 2019
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Xylan from bambara and cowpea biomass and their structural elucidation.

Int J Biol Macromol 2019 Apr 5;132:987-993. Epub 2019 Apr 5.

Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, PO BOX 1334, Durban 4000, South Africa. Electronic address:

This work is the first report on the isolation and structural elucidation of xylan from bambara and cowpea biomass. The xylans, isolated using acidic delignification followed by NaOH extraction method gave 12.3% and 13. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.030DOI Listing

Total mercury, chromium, nickel and other trace chemical element contents in soils at an old cinnabar mine site (Merník, Slovakia): anthropogenic versus natural sources of soil contamination.

Environ Monit Assess 2019 Apr 5;191(5):263. Epub 2019 Apr 5.

GEOtest, a.s., Šmahova 1244/112, 627 00, Brno, Czech Republic.

The aims of this study were to investigate the occurrence and distribution of total mercury (Hg) and other trace elements of environmental concern, such as arsenic (As), copper (Cu), chromium (Cr), manganese (Mn), nickel (Ni), lead (Pb), zinc (Zn) and vanadium (V), in soils from the abandoned Merník cinnabar mine in eastern Slovakia. For this purpose, thirty soil samples from two depth intervals within the mine area (n = 60 soil samples) and additional sixteen soil samples from adjacent areas (n = 25 soil samples) were collected. Total Hg was measured by atomic absorption spectrometry, while As and other metals were analyzed using inductively coupled plasma atomic emission spectrometry. Read More

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http://dx.doi.org/10.1007/s10661-019-7391-6DOI Listing

Circulating Tumor Cells With Epithelial-to-mesenchymal Transition Phenotypes Associated With Inferior Outcomes in Primary Breast Cancer.

Anticancer Res 2019 Apr;39(4):1829-1837

2nd Department of Oncology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic.

Background/aim: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients.

Patients And Methods: This study included 427 primary breast cancer patients. Read More

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http://dx.doi.org/10.21873/anticanres.13290DOI Listing
April 2019
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Toxicity of food contact paper evaluated by combined biological and chemical methods.

Toxicol In Vitro 2019 Apr 2;59:26-34. Epub 2019 Apr 2.

National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic.

The study was focused on assessment of potential health risks of paper-based food contact materials (FCMs) in a step-wise approach using three toxicological bioassays in vitro and chemical analyses of migrating contaminants. 3T3 NRU cytotoxicity test showed high sensitivity to detect basal toxicity of FCMs extracts and served as a first-line test for selection of samples for further testing. The reconstructed human intestine model EpiIntestinal showed more realistic tissue response than cell culture monolayer and higher resistance despite prolonged exposure to the selected 6 samples, i. Read More

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http://dx.doi.org/10.1016/j.tiv.2019.04.001DOI Listing

The membrane structure and function affected by water.

Chem Phys Lipids 2019 Apr 2;221:140-144. Epub 2019 Apr 2.

Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Slovakia.

Various experimental data reveal intriguing peculiarities in structural properties of biomimetic membranes. Interestingly, one of the common alterations that is observed at the membrane-water interface underlines the important role of membrane hydration properties. A plausible mechanism of action in the case of many membrane additives seems to be in shifting the water encroachment the way that bilayers absorb more or less water molecules - one of the smallest and often neglected biomolecule. Read More

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http://dx.doi.org/10.1016/j.chemphyslip.2019.04.002DOI Listing

Magnetic Resonance Imaging of the Musculoskeletal System at 7T: Morphological Imaging and Beyond.

Top Magn Reson Imaging 2019 Apr 2. Epub 2019 Apr 2.

High-field MR Center, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria.

In 2017, a whole-body 7T magnetic resonance imaging (MRI) device was given regulatory approval for clinical use in both the EU and United States for neuro and musculoskeletal applications. As 7 Tesla allows for higher signal-to-noise , which results in higher resolution images than those obtained on lower-field-strength scanners, it has attracted considerable attention from the musculoskeletal field, as evidenced by the increasing number of publications in the last decade. Besides morphological imaging, the quantitative MR methods, such as T2, T2*, T1ρ mapping, sodium imaging, chemical-exchange saturation transfer, and spectroscopy, substantially benefit from ultrahigh-field scanning. Read More

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http://dx.doi.org/10.1097/RMR.0000000000000205DOI Listing
April 2019
3 Reads

Overview and recent advances in electrochemical sensing of glutathione - A review.

Anal Chim Acta 2019 Jul 7;1062:1-27. Epub 2019 Mar 7.

Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Odbojárov 10, SK-832 32, Bratislava, Slovak Republic; Comenius University in Bratislava, Faculty of Pharmacy, Toxicological and Antidoping Center, Odbojárov 10, SK-832 32, Bratislava, Slovak Republic. Electronic address:

The present paper is aimed at providing an overview of the recent advances in the electrochemical sensing of glutathione (GSH), an important electrochemically and biologically active molecule, for the period 2012-2018. Herein, the analytical performances of newly developed electrochemical methods, procedures and protocols for GSH sensing are comprehensively and critically discussed with respect to the type of method, electrodes used (new electrode modifications, advanced materials and formats), sample matrices, and basic validation parameters obtained (limit of detection, linear dynamic range, precision, selectivity/evaluation of interferences). This paper considers electrochemical methods used alone as well as the hyphenated methods with electrochemical detection (ECD), such as HPLC-ECD or CE-ECD. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.052DOI Listing
July 2019
2 Reads

Dietary bioflavonoid quercetin modulates porcine ovarian granulosa cell functions in vitro.

J Environ Sci Health B 2019 Apr 4:1-5. Epub 2019 Apr 4.

f Department of Nutrition, Faculty of Agrobiology and Food Resources , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.

Quercetin is a dietary bioflavonoid used widely as a food supplement and is generally recognized as safe. The aim of this in vitro study was to examine the steroid hormone (progesterone and 17- β estradiol) release, proliferation (PCNA and cyclin B1) and apoptosis (caspase 3 and p53) of porcine ovarian granulosa cells after the addition of quercetin at concentrations 0.01, 0. Read More

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http://dx.doi.org/10.1080/03601234.2019.1586034DOI Listing

Effects of ginger constituent 6-shogaol on gastroesophageal vagal afferent C-fibers.

Neurogastroenterol Motil 2019 Apr 4:e13585. Epub 2019 Apr 4.

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Background: Ginger has been used as an herbal medicine worldwide to relieve nausea/vomiting and gastrointestinal discomfort, but the cellular and molecular mechanisms of its neuronal action remain unclear. The present study aimed to determine the effects of ginger constituent 6-shogaol on gastroesophageal vagal nodose C-fibers.

Methods: Extracellular single-unit recording and two-photon nodose neuron imaging were performed, respectively, in ex vivo gastroesophageal-vagal preparations from wild type and Pirt-GCaMP6 transgenic mice. Read More

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http://dx.doi.org/10.1111/nmo.13585DOI Listing
April 2019
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Fibro-osseous Pseudotumor of digits and myositis Ossificans show consistent COL1A1-USP6 rearrangement: a Clinicopathological and genetic study of 27 cases.

Hum Pathol 2019 Apr 1. Epub 2019 Apr 1.

Šikl's Department of Pathology, Charles University in Prague, The Faculty of Medicine and Faculty Hospital in Pilsen, Alej Svobody 80, 304 60 Pilsen, Czech Republic; Bioptická laboratoř s.r.o., Mikulášske nám. 4, 326 00, Pilsen.

Myositis ossificans (MO) and fibro-osseous pseudotumor of digits (FOPD) are localized, self-limiting bone-producing pseudosarcomatous lesions characterized by nodular fasciitis-like proliferation and osteoid and immature woven bone production, which may eventually develop into more mature lamelar bone. Traditionally, MO/FOPD were thought to be of reactive, non-neoplastic nature. USP6 gene rearrangement was recently reported as a consistent finding in MO and FOPD, thus expanding the spectrum of transient, USP6-rearranged neoplasms. Read More

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http://dx.doi.org/10.1016/j.humpath.2019.02.009DOI Listing
April 2019
1 Read